MD07G1183300.v1.1

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017364
Physical Location & Seq
Forward (+)
26336656 .. 26337180
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1183300.v1.1.491

Sequence Viewer

Length: 525 bp
ATGAAGAGACTACGCATCTTAACCGTCGTTGCAATAGCCTTCATCACTTCTCTGCCAGTACAAGGATCCAGCACAAACTGCTCCTACTCCGTCGAGATTGAGACAACGTGTGCACAATCAGCCGCAACCTCGGACCATGTCAGCGTCAGGTTCGGGGACTCCGAGGGCAATTTGATCATAGTGAAGCATCTGAACAACCCTAAGCCATTGTATGCTGCAAAAGGTGGTGTGAGACGTGGGGGGTATGGCGGGTTTGGACGATGTGCCATAGACATGTTTGAGGCCAGTGGACAATGCATGAGCCGGAGGGTGTGCTATCTGTACCTGAAGAAGGTTGGGTCGGACGACTGGCGGCCTGGCTGGGTGAGGGTGCTTCATCGACAGGATGGCGGCCGGGTGGTAGTGCCGGTTTCTTACATGTTCTATTTTAGAACGTTTGTTCCAGAAAACGTTTGGTATGGGTTTGATTATTGTCATTCCAAAGGTGGTGGTTTTGTGCCCCATGTTGTTAGTTCTAATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

175

Amino Acids

19.3

Weight (kDa)

9.22

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 21 - 158 1.1e-38 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017364)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20661
malus_domestica MD07G1183300.v1.1
prunus_persica Prupe.2G222000_v2.0.a1
pyrus_communis pycom07g17590
rosa_chinensis RchiOBHm_Chr1g0364691
rosa_laevigata RLG00000027466
rosa_multiflora Rmu_ssc0000112.1_g000040
rosa_roxburghii Rroxscaffold_4G00291740
rosa_rugosa Rorug01G0318200
rosa_samantha Rh1AG326900 Rh1BG288000 Rh1DG319800
rosa_wichuraiana Rw1G028960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 123, 249, 352, 390
AclI AACGTT 2 cut(s) 434, 450
AclWI GGATC 2 cut(s) 60, 73
AcoI YGGCCR 1 cut(s) 391
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 2 cut(s) 60, 323
AfiI CCNNNNNNNGG 2 cut(s) 62, 331
AflIII ACRYGT 3 cut(s) 107, 273, 417
AjiI CACGTC 1 cut(s) 236
AjnI CCWGG 1 cut(s) 355
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 95, 226
Alw44I GTGCAC 1 cut(s) 111
AlwI GGATC 2 cut(s) 60, 73
AoxI GGCC 3 cut(s) 282, 353, 391
ApaLI GTGCAC 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 2 cut(s) 115, 501
BamHI GGATCC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 2 cut(s) 95, 226
BisI GCNGC 4 cut(s) 123, 216, 353, 391
BlsI GCNGC 4 cut(s) 124, 217, 354, 392
Bme1390I CCNGG 2 cut(s) 357, 395
Bme18I GGWCC 1 cut(s) 133
BmgBI CACGTC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 2 cut(s) 357, 395
BmsI GCATC 2 cut(s) 24, 196
Bpu10I CCTNAGC 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 129, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 331
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 3 cut(s) 56, 285, 353
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 129, 162
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 2 cut(s) 62, 331
BseNI ACTGG 3 cut(s) 56, 285, 353
BseSI GKGCMC 2 cut(s) 115, 501
BseX3I CGGCCG 1 cut(s) 391
BseXI GCAGC 1 cut(s) 202
BseYI CCCAGC 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 3 cut(s) 284, 355, 393
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 3 cut(s) 304, 394, 407
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 62, 331
BsmAI GTCTC 2 cut(s) 95, 226
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 3 cut(s) 284, 355, 393
Bsp1286I GDGCHC 2 cut(s) 115, 501
Bsp143I GATC 2 cut(s) 65, 174
BspACI CCGC 4 cut(s) 123, 249, 352, 390
BspANI GGCC 3 cut(s) 284, 355, 393
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 2 cut(s) 60, 73
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 3 cut(s) 56, 285, 353
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 129, 162
BssMI GATC 2 cut(s) 65, 174
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 25
BstAPI GCANNNNNTGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 2 cut(s) 68, 177
BstMAI GTCTC 2 cut(s) 95, 226
BstMBI GATC 2 cut(s) 65, 174
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 2 cut(s) 78, 119
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 2 cut(s) 277, 421
BstSCI CCNGG 2 cut(s) 355, 393
BstSLI GKGCMC 2 cut(s) 115, 501
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BstZI CGGCCG 1 cut(s) 391
BsuRI GGCC 3 cut(s) 284, 355, 393
BtrI CACGTC 1 cut(s) 236
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 1 cut(s) 133
Csp6I GTAC 2 cut(s) 59, 322
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 5 cut(s) 137, 274, 298, 418, 503
CviJI RGCY 8 cut(s) 38, 122, 205, 284, 303, 355, 360, 393
CviKI_1 RGCY 8 cut(s) 38, 122, 205, 284, 303, 355, 360, 393
CviQI GTAC 2 cut(s) 59, 322
DdeI CTNAG 1 cut(s) 201
DpnI GATC 2 cut(s) 67, 176
DpnII GATC 2 cut(s) 65, 174
EaeI YGGCCR 1 cut(s) 391
EagI CGGCCG 1 cut(s) 391
EclXI CGGCCG 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 133
Eco52I CGGCCG 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 355
EcoT22I ATGCAT 1 cut(s) 299
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 5 cut(s) 140, 277, 301, 421, 506
FaqI GGGAC 1 cut(s) 170
FatI CATG 5 cut(s) 136, 273, 297, 417, 502
FauI CCCGC 1 cut(s) 242
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 4 cut(s) 123, 216, 353, 391
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 4 cut(s) 123, 216, 353, 391
GluI GCNGC 4 cut(s) 123, 216, 353, 391
GsaI CCCAGC 1 cut(s) 364
HaeIII GGCC 3 cut(s) 284, 355, 393
HapII CCGG 3 cut(s) 304, 394, 407
HgaI GACGC 1 cut(s) 133
Hin1II CATG 5 cut(s) 140, 277, 301, 421, 506
HinfI GANTC 1 cut(s) 158
HpaII CCGG 3 cut(s) 304, 394, 407
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 2 cut(s) 113, 290
Hpy188I TCNGA 4 cut(s) 133, 163, 192, 343
Hpy188III TCNNGA 2 cut(s) 94, 443
Hpy8I GTNNAC 2 cut(s) 113, 290
Hpy99I CGWCG 2 cut(s) 29, 95
HpyAV CCTTC 2 cut(s) 49, 325
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 4 cut(s) 107, 235, 434, 450
HpyCH4V TGCA 4 cut(s) 32, 113, 218, 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 119
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 4 cut(s) 107, 235, 434, 450
Hsp92II CATG 5 cut(s) 140, 277, 301, 421, 506
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 2 cut(s) 65, 174
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 24, 196
MaeII ACGT 4 cut(s) 107, 235, 434, 450
MalI GATC 2 cut(s) 67, 176
MboI GATC 2 cut(s) 65, 174
MboII GAAGA 2 cut(s) 16, 340
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 2 cut(s) 115, 501
MluCI AATT 1 cut(s) 169
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 5 cut(s) 139, 157, 274, 300, 360
Mph1103I ATGCAT 1 cut(s) 299
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 272
MspI CCGG 3 cut(s) 304, 394, 407
MspR9I CCNGG 2 cut(s) 357, 395
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 78, 119
NciI CCSGG 1 cut(s) 395
NdeII GATC 2 cut(s) 65, 174
NlaIII CATG 5 cut(s) 140, 277, 301, 421, 506
NlaIV GGNNCC 1 cut(s) 67
NsiI ATGCAT 1 cut(s) 299
NspI RCATGY 2 cut(s) 277, 421
PciI ACATGT 2 cut(s) 273, 417
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 4 cut(s) 124, 217, 354, 392
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 2 cut(s) 273, 417
Psp1406I AACGTT 2 cut(s) 434, 450
Psp6I CCWGG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 360
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 2 cut(s) 60, 323
RsaNI GTAC 2 cut(s) 59, 322
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 4 cut(s) 123, 216, 353, 391
Sau3AI GATC 2 cut(s) 65, 174
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 2 cut(s) 357, 395
SduI GDGCHC 2 cut(s) 115, 501
SfaNI GCATC 2 cut(s) 24, 196
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 272
Sse9I AATT 1 cut(s) 169
SsiI CCGC 4 cut(s) 123, 249, 352, 390
StyD4I CCNGG 2 cut(s) 355, 393
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 4 cut(s) 110, 238, 437, 453
TaqI TCGA 2 cut(s) 93, 379
TasI AATT 1 cut(s) 169
TatI WGTACW 1 cut(s) 58
TauI GCSGC 3 cut(s) 125, 355, 393
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 292
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 3 cut(s) 17, 31, 365
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 137
VneI GTGCAC 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 329
XceI RCATGY 2 cut(s) 277, 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 450
Zsp2I ATGCAT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.