Prupe.2G222000_v2.0.a1

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0017364
Physical Location & Seq
Forward (+)
24974995 .. 24975525
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G222000.1

Sequence Viewer

Length: 504 bp
ATGAAGAGAATGAGCATTTTAACTGTCTTCGCAATTTTCTTCATCATTTCTATGCCAATCAATGCAATGCAACCTAACAACTATGGCTTCCAAGGATCCAAAACAAACTGCACCTACTCCATTGAGATTGAGACAACATGTGCACAATCTGCCGGAACCACGGACCATGTCAGTGTCAGATTCGGGGACTCTGAGGGTAACTTGATCATAGTGAAGCATCTGAACAACCCTAAGCTATTGTATGCTCCAAAGGGTGGTTTGAGACGTAGGGGTTATGGTGGGTTTGGACGATGTGCCAAAGACATGTTTGAGGCCAGTGGACCATGCATGAGCCAGTGGGTGTGCTCTCTGTACCTGAAGAAGGTCGGCTCAGACGACTGGCGACCTGGCCGGGTGAAGGTGCTTCATCAACAAGATGGCAGCCGTGTAGTGCCAGTTTCTTATGTGTTCTATTTTAGGACATTTGTTCCAGAAAATGTTTGGTATGGGTTTAATTATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

168

Amino Acids

18.88

Weight (kDa)

9.3

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017364)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20661
malus_domestica MD07G1183300.v1.1
prunus_persica Prupe.2G222000_v2.0.a1
pyrus_communis pycom07g17590
rosa_chinensis RchiOBHm_Chr1g0364691
rosa_laevigata RLG00000027466
rosa_multiflora Rmu_ssc0000112.1_g000040
rosa_roxburghii Rroxscaffold_4G00291740
rosa_rugosa Rorug01G0318200
rosa_samantha Rh1AG326900 Rh1BG288000 Rh1DG319800
rosa_wichuraiana Rw1G028960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AclWI GGATC 2 cut(s) 90, 103
AcoI YGGCCR 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 254, 361, 397
AflIII ACRYGT 2 cut(s) 137, 303
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
Alw21I GWGCWC 2 cut(s) 145, 347
Alw26I GTCTC 2 cut(s) 125, 256
Alw44I GTGCAC 1 cut(s) 141
AlwI GGATC 2 cut(s) 90, 103
AoxI GGCC 2 cut(s) 312, 388
ApaLI GTGCAC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 163, 320
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 406
AvaII GGWCC 2 cut(s) 163, 320
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 145, 347
BbvI GCAGC 1 cut(s) 432
BccI CCATC 1 cut(s) 410
BceAI ACGGC 1 cut(s) 408
BciT130I CCWGG 1 cut(s) 387
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 2 cut(s) 125, 256
BisI GCNGC 1 cut(s) 421
BlsI GCNGC 1 cut(s) 422
Bme1390I CCNGG 2 cut(s) 387, 392
Bme18I GGWCC 2 cut(s) 163, 320
BmgT120I GGNCC 2 cut(s) 163, 320
BmiI GGNNCC 2 cut(s) 97, 157
BmrFI CCNGG 2 cut(s) 387, 392
BmsI GCATC 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 91, 159
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 361, 397
Bse1I ACTGG 4 cut(s) 315, 334, 383, 434
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 91, 159
BseLI CCNNNNNNNGG 3 cut(s) 254, 361, 397
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 2 cut(s) 183, 384
BseNI ACTGG 4 cut(s) 315, 334, 383, 434
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 432
BsgI GTGCAG 1 cut(s) 94
BshFI GGCC 2 cut(s) 314, 390
BsiHKAI GWGCWC 2 cut(s) 145, 347
BsiSI CCGG 2 cut(s) 153, 391
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 3 cut(s) 254, 361, 397
BsmAI GTCTC 2 cut(s) 125, 256
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 314, 390
Bsp1286I GDGCHC 2 cut(s) 145, 347
Bsp143I GATC 2 cut(s) 95, 204
BspANI GGCC 2 cut(s) 314, 390
BspCNI CTCAG 2 cut(s) 184, 383
BspLI GGNNCC 2 cut(s) 97, 157
BspPI GGATC 2 cut(s) 90, 103
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 4 cut(s) 315, 334, 383, 434
BssECI CCNNGG 2 cut(s) 91, 159
BssMI GATC 2 cut(s) 95, 204
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 25
BstAPI GCANNNNNTGC 1 cut(s) 149
BstDEI CTNAG 3 cut(s) 192, 231, 370
BstDSI CCRYGG 1 cut(s) 159
BstENI CCTNNNNNAGG 1 cut(s) 359
BstKTI GATC 2 cut(s) 98, 207
BstMAI GTCTC 2 cut(s) 125, 256
BstMBI GATC 2 cut(s) 95, 204
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 2 cut(s) 141, 307
BstSCI CCNGG 2 cut(s) 385, 390
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 432
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 314, 390
BtgI CCRYGG 1 cut(s) 159
BtsIMutI CAGTG 3 cut(s) 178, 322, 341
Cfr13I GGNCC 2 cut(s) 163, 320
Csp6I GTAC 1 cut(s) 352
CviAII CATG 5 cut(s) 138, 167, 304, 324, 328
CviJI RGCY 7 cut(s) 87, 235, 314, 333, 369, 390, 423
CviKI_1 RGCY 7 cut(s) 87, 235, 314, 333, 369, 390, 423
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 3 cut(s) 192, 231, 370
DpnI GATC 2 cut(s) 97, 206
DpnII GATC 2 cut(s) 95, 204
EaeI YGGCCR 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 163, 320
Eco57I CTGAAG 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 91
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 5 cut(s) 141, 170, 307, 327, 331
FaqI GGGAC 1 cut(s) 200
FatI CATG 5 cut(s) 137, 166, 303, 323, 327
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 421
Fsp4HI GCNGC 1 cut(s) 421
GluI GCNGC 1 cut(s) 421
HaeIII GGCC 2 cut(s) 314, 390
HapII CCGG 2 cut(s) 153, 391
Hin1II CATG 5 cut(s) 141, 170, 307, 327, 331
HinfI GANTC 2 cut(s) 180, 188
HpaII CCGG 2 cut(s) 153, 391
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 143, 320
Hpy188I TCNGA 4 cut(s) 179, 193, 222, 373
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 2 cut(s) 143, 320
HpyAV CCTTC 2 cut(s) 355, 391
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 5 cut(s) 65, 70, 111, 143, 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 3 cut(s) 192, 231, 370
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 5 cut(s) 141, 170, 307, 327, 331
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 2 cut(s) 95, 204
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 432
LweI GCATC 1 cut(s) 226
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 1 cut(s) 197
MalI GATC 2 cut(s) 97, 206
MboI GATC 2 cut(s) 95, 204
MboII GAAGA 4 cut(s) 16, 19, 31, 370
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 2 cut(s) 145, 347
MluCI AATT 2 cut(s) 33, 493
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 2 cut(s) 187, 304
Mph1103I ATGCAT 1 cut(s) 329
MseI TTAA 2 cut(s) 20, 492
MslI CAYNNNNRTG 2 cut(s) 50, 171
MspI CCGG 2 cut(s) 153, 391
MspR9I CCNGG 2 cut(s) 387, 392
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 149
NciI CCSGG 1 cut(s) 392
NdeII GATC 2 cut(s) 95, 204
NlaIII CATG 5 cut(s) 141, 170, 307, 327, 331
NlaIV GGNNCC 2 cut(s) 97, 157
NsiI ATGCAT 1 cut(s) 329
NspI RCATGY 2 cut(s) 141, 307
PciI ACATGT 2 cut(s) 137, 303
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PfeI GAWTC 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 422
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PscI ACATGT 2 cut(s) 137, 303
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 97, 157
PspPI GGNCC 2 cut(s) 163, 320
PsuI RGATCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 2 cut(s) 50, 171
SaqAI TTAA 2 cut(s) 20, 492
SatI GCNGC 1 cut(s) 421
Sau3AI GATC 2 cut(s) 95, 204
Sau96I GGNCC 2 cut(s) 163, 320
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 387, 392
SduI GDGCHC 2 cut(s) 145, 347
SetI ASST 8 cut(s) 76, 116, 237, 268, 357, 366, 388, 402
SfaNI GCATC 1 cut(s) 226
SinI GGWCC 2 cut(s) 163, 320
SmiMI CAYNNNNRTG 2 cut(s) 50, 171
Sse9I AATT 2 cut(s) 33, 493
StyD4I CCNGG 2 cut(s) 385, 390
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 268
TasI AATT 2 cut(s) 33, 493
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 2 cut(s) 20, 492
Tru9I TTAA 2 cut(s) 20, 492
TscAI CASTG 3 cut(s) 178, 322, 341
TseI GCWGC 1 cut(s) 420
TspDTI ATGAA 3 cut(s) 17, 31, 395
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 3 cut(s) 178, 322, 341
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 254
VneI GTGCAC 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 163, 320
XagI CCTNNNNNAGG 1 cut(s) 359
XceI RCATGY 2 cut(s) 141, 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.