Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002101

Physical Location & Seq

Genomic Coordinates Forward (+)

30668540 .. 30672696

Gene Structure 4157 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1232700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGGATCTTGAGAGAATTAAGTGTTGGGACACTTCAAAGAAGGATTCTTGGAAGAATGTTATGCTCTTGGCCTACCAGAGCCTTGGAGTAGTGTATGGTGACCTGAGCATTTCCCCTCTCTATGTTTACAAGAGCGCATTTGCAGAAGACATTCAACATTCGGATACGAATGAAGAGATTTATGGTGTACTTTCTTTTGTCTTCTGGACTCTTACTTTAGTTCCGCTGTTCAAGTATGTCTTTATAGTCCTTCGAGCTGATGATAATGGAGAGGGTGGTACATTTGCACTGTATTCGCTGATATGTAGGCACGCCAAAGTAAGCCTTCTCCCCAACCGCCAGCTTGCTGATGAGGCACTTTCTACTTATAAACTGGAGCACCCACCAGAGAAGGAAAAAAGTTCCAGGGTGAAAGTTGTACTCGAGAAGTGCAAGACCTTGCACACTGCCTTGCTAATTCTTGTTCTTCTTGGAACTTGTATGGTAATTGGTGATGGAGTGCTCACTCCAGCAATTTCAGTCTTTTCTGCGGTGTCTGGCCTGGAATTATCCATGTCCAAGGAGCATCACCAATATGCTGTGGTTCCAATTACTTGTTTCATATTGTTGTGTTTATTTGCACTTCAACACTATGGCACCCATCGAATCGGATTCCTTTTTGCACCAGTGGTGTTGGCATGGCTTCTGTGCATCAGTGCTCTTGGCTTGTATAATATAATCCACTGGAACCGTTATGTCTATCATGCTCTGTCTCCGTATTACATGTTCAAGTTCTTGAGGAAGACAAGGATAAGTGGCTGGATGTCTTTGGGTGGAATATTGCTGTGCATAACAGGCTCAGAGGCAATGTTTGCTGATCTTGGCCACTTCTCATATAATGCCATTCAGGTTGCTTTTACCTTTTTGGTTTATCCAGCTCTTATATTGGCATATATGGGGCAGGCTGCTTACTTATCGCAGCATCACCACAGCAGTCACCAGATCAGCTTTTATGTCTCGGTTCCAGAAACTGTGAGATGGCCCGTGCTTGTACTAGCCATTCTTGCTTCTGTTGTGGGAAGCCAAGCAATCATCAGCGGAACATTTTCTATCATAAACCAGAGCCAATCATTAAGTTGTTTTCCGAGAGTCAAGGTCGTCCACACTTCTGACAAGGTACATGGCCAGATATACATCCCCGAGATCAATTGGATGCTCATGATCCTCTGCATTGCAGTGACCATTGGATTTAGAGACACAAAACACCTGGGAAATGCTTCTGGGCTAGCAGTGATGACGGTTATGTTGGTTACCACATGCCTCACTTCCTTGGTGATTGTCATTTGTTGGCACAAGCCACCTATTGTAGCCCTTTGCTTCCTAATCTTCTTTGGCTCCATTGAATTGCTCTACTTCTCAGCTTCGCTCACCAAGTTCCGAGAGGGTGCCTGGCTTCCCATCCTTCTTGCGCTGTTCCTCATGACTATCATGTTTGTATGGCATTATGCAACCATCAAGAAGTATGAATATGACCTCCACAACAAGGTTTCATTAGACTGGCTTCTAGCTTTGGGTCCAAGCTTGGGAATCGCTCGGGTCCCTGGCATTGGCCTAGTGTTCACAGATCTCACCTCCGGCATTCCTGCTAACTTCTCCCGCTTTGTAACCAACCTCCCTGCTTTCCACCGCGTCTTAGTTTTCGTGTGTGTAAAATCAGTCCCTGTCCCTTATGTGCCGCCTGCTGAGCGATACTTGGTGGGTCGCGTTGGTCCTGCAGCTCATCGGTCCTACAGGTGCATTGTTCGTTATGGATACCGTGATGTCCATCAAGATGTTGACTCCTTTGAGACAGAACTAGTTGAAAGGCTGGTTGATTTCATCCAATATGACTGGTGTCGAACACAGAGAACTAGCTCCTGCACTGAGGATAACGCATCGAGGTCTACTGACACGAGCGAGTGTAGACTAGCTGTCATCGGAACAGTATCATTTTCTGGTGCACCAGCTTATGAGATTGAGGAGACTCAACCGGCAAGTGTATCTGTTGGTTTCCCAACTGTAGAAAGCATAACAGACGTTGTTGAGATGGAACCGGTTGAAAGAAGAGTGAGGTTTGCCATCGACGAGGAGTCTCAGCCTGACACCCAGCCTGAGAATACAACGCTGATACGCGAAGAGCTTGAGGATCTCTACGCAGCTCAAGAGGCTGGGACTGCATTTATACTTGGGCACTCACACGTTAAAGCGAAGCAAGGCTCGTCGTTAATGAAGAGATTCGCCATTAACTTCGGTTATAACTGCCTGAGGAGGAATTGCCGGGGGCCAGACGTGGCGCTGAAGGTCCCGCCGGAGTCTCTTCTCGAGGTTGGCATGGTTTATGTTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

789

Amino Acids

88.3

Weight (kDa)

6.7

Isoelectric Point (pI)

41.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	23 - 515	6.7e-171	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	527 - 786	1.2e-29	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002101)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40540 AT2G40540 AT2G40540 AT2G40540 AT2G40540
fragaria_vesca	FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830 FvH4_7g25830
malus_domestica	MD01G1165900.v1.1 MD07G1232700.v1.1
prunus_persica	Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1 Prupe.2G263200_v2.0.a1
pyrus_communis	pycom01g17950 pycom07g21040
rosa_chinensis	RchiOBHm_Chr1g0372091
rosa_laevigata	RLG00000026924
rosa_multiflora	Rmu_sc0001755.1_g000029
rosa_roxburghii	Rroxscaffold_4G00285270
rosa_rugosa	Rorug01G0369200 Rorug01G0369300 Rorug01G0369400 Rorug01G0369500 Rorug01G0369600 Rorug01G0369600
rosa_samantha	Rh1AG377900 Rh1BG341300 Rh1CG354600 Rh1DG372400
rosa_wichuraiana	Rw1G033250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	369, 2274
AccB1I	GGYRCC	2 cut(s)	635, 1424
AccI	GTMKAC	2 cut(s)	1922, 1942
AccII	CGCG	3 cut(s)	1668, 1743, 2151
AciI	CCGC	8 cut(s)	224, 337, 530, 1077, 1636, 1666, 1715, 2324
AclWI	GGATC	3 cut(s)	12, 1195, 2172
AcoI	YGGCCR	2 cut(s)	862, 1162
AcuI	CTGAAG	1 cut(s)	2336
AfaI	GTAC	5 cut(s)	189, 280, 420, 1032, 1158
AfiI	CCNNNNNNNGG	3 cut(s)	1522, 1562, 1586
AflIII	ACRYGT	2 cut(s)	762, 2215
AgeI	ACCGGT	1 cut(s)	2071
AgsI	TTSAA	8 cut(s)	36, 155, 232, 626, 769, 1382, 1841, 2078
AhdI	GACNNNNNGTC	2 cut(s)	1949, 2107
AhlI	ACTAGT	1 cut(s)	1834
AjiI	CACGTC	1 cut(s)	2308
AjnI	CCWGG	5 cut(s)	404, 540, 1245, 1427, 1579
AjuI	GAANNNNNNNTTGG	2 cut(s)	7, 39
Alw21I	GWGCWC	4 cut(s)	381, 504, 700, 1981
Alw26I	GTCTC	7 cut(s)	756, 1000, 1227, 1820, 1994, 2115, 2337
Alw44I	GTGCAC	1 cut(s)	1977
AlwI	GGATC	3 cut(s)	12, 1195, 2172
AlwNI	CAGNNNCTG	2 cut(s)	1010, 1700
Ama87I	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
AoxI	GGCC	7 cut(s)	69, 538, 862, 1019, 1162, 1588, 2300
ApaLI	GTGCAC	1 cut(s)	1977
ApeKI	GCWGC	4 cut(s)	944, 958, 1754, 2174
AsiGI	ACCGGT	1 cut(s)	2071
Asp700I	GAANNNNTTC	4 cut(s)	150, 1084, 1255, 2252
AspLEI	GCGC	3 cut(s)	137, 1450, 2314
AspS9I	GGNCC	7 cut(s)	1020, 1553, 1576, 1748, 1764, 2300, 2320
AsuC2I	CCSGG	1 cut(s)	2297
AsuHPI	GGTGA	9 cut(s)	110, 421, 503, 560, 956, 968, 1324, 1399, 1600
AsuNHI	GCTAGC	1 cut(s)	1264
AvaI	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
AvaII	GGWCC	5 cut(s)	1553, 1576, 1748, 1764, 2320
AxyI	CCTNAGG	1 cut(s)	2282
BaeGI	GKGCMC	2 cut(s)	1981, 2211
BalI	TGGCCA	2 cut(s)	864, 1164
BanI	GGYRCC	2 cut(s)	635, 1424
BauI	CACGAG	1 cut(s)	1930
BbsI	GAAGAC	3 cut(s)	153, 193, 788
Bbv12I	GWGCWC	4 cut(s)	381, 504, 700, 1981
BbvI	GCAGC	4 cut(s)	931, 970, 1766, 2186
BccI	CCATC	8 cut(s)	488, 648, 1011, 1445, 1499, 1812, 2059, 2105
BcgI	CGANNNNNNTGC	2 cut(s)	276, 310
BciT130I	CCWGG	5 cut(s)	406, 542, 1247, 1429, 1581
BciVI	GTATCC	2 cut(s)	157, 1784
BcnI	CCSGG	1 cut(s)	2297
BcoDI	GTCTC	7 cut(s)	756, 1000, 1227, 1820, 1994, 2115, 2337
BcuI	ACTAGT	1 cut(s)	1834
BfaI	CTAG	7 cut(s)	1034, 1265, 1544, 1592, 1835, 1890, 1946
BfmI	CTRYAG	3 cut(s)	1752, 1768, 2037
BfoI	RGCGCY	1 cut(s)	2315
BfuI	GTATCC	2 cut(s)	157, 1784
BglII	AGATCT	1 cut(s)	1603
BisI	GCNGC	5 cut(s)	945, 959, 1715, 1755, 2175
BlpI	GCTNAGC	1 cut(s)	1722
BlsI	GCNGC	5 cut(s)	946, 960, 1716, 1756, 2176
Bme1390I	CCNGG	6 cut(s)	406, 542, 1247, 1429, 1581, 2297
Bme18I	GGWCC	5 cut(s)	1553, 1576, 1748, 1764, 2320
BmeRI	GACNNNNNGTC	2 cut(s)	1949, 2107
BmeT110I	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
BmgBI	CACGTC	1 cut(s)	2308
BmgT120I	GGNCC	7 cut(s)	1020, 1553, 1576, 1748, 1764, 2300, 2320
BmrFI	CCNGG	6 cut(s)	406, 542, 1247, 1429, 1581, 2297
BmsI	GCATC	5 cut(s)	574, 699, 970, 1182, 1922
BmtI	GCTAGC	1 cut(s)	1268
BoxI	GACNNNNGTC	1 cut(s)	1872
BpiI	GAAGAC	3 cut(s)	153, 193, 788
BpmI	CTGGAG	2 cut(s)	395, 492
Bpu10I	CCTNAGC	1 cut(s)	104
Bpu1102I	GCTNAGC	1 cut(s)	1722
BpuEI	CTTGAG	4 cut(s)	29, 796, 2163, 2180
BpuMI	CCSGG	1 cut(s)	2297
BsaBI	GATNNNNATC	2 cut(s)	1172, 1803
BsaJI	CCNNGG	7 cut(s)	82, 405, 558, 1246, 1308, 1579, 2296
BsaWI	WCCGGW	1 cut(s)	2071
Bsc4I	CCNNNNNNNGG	3 cut(s)	1522, 1562, 1586
Bse118I	RCCGGY	2 cut(s)	2008, 2071
Bse1I	ACTGG	5 cut(s)	378, 665, 728, 1541, 1874
Bse21I	CCTNAGG	1 cut(s)	2282
Bse3DI	GCAATG	2 cut(s)	852, 1209
Bse8I	GATNNNNATC	2 cut(s)	1172, 1803
BseBI	CCWGG	5 cut(s)	406, 542, 1247, 1429, 1581
BseDI	CCNNGG	7 cut(s)	82, 405, 558, 1246, 1308, 1579, 2296
BseGI	GGATG	5 cut(s)	807, 1173, 1197, 1437, 1857
BseJI	GATNNNNATC	2 cut(s)	1172, 1803
BseLI	CCNNNNNNNGG	3 cut(s)	1522, 1562, 1586
BseMI	GCAATG	2 cut(s)	852, 1209
BseMII	CTCAG	8 cut(s)	95, 852, 1410, 1713, 1893, 2121, 2126, 2273
BseNI	ACTGG	5 cut(s)	378, 665, 728, 1541, 1874
BseRI	GAGGAG	3 cut(s)	2012, 2120, 2299
BseSI	GKGCMC	2 cut(s)	1981, 2211
BseXI	GCAGC	4 cut(s)	931, 970, 1766, 2186
BseYI	CCCAGC	2 cut(s)	2124, 2186
BsgI	GTGCAG	1 cut(s)	1882
Bsh1236I	CGCG	3 cut(s)	1668, 1743, 2151
BshFI	GGCC	7 cut(s)	71, 540, 864, 1021, 1164, 1590, 2302
BshNI	GGYRCC	2 cut(s)	635, 1424
BshTI	ACCGGT	1 cut(s)	2071
BsiHKAI	GWGCWC	4 cut(s)	381, 504, 700, 1981
BsiHKCI	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
BsiSI	CCGG	5 cut(s)	1614, 2009, 2072, 2296, 2327
BslFI	GGGAC	6 cut(s)	41, 1562, 1682, 1688, 2203, 2306
BslI	CCNNNNNNNGG	3 cut(s)	1522, 1562, 1586
BsmAI	GTCTC	7 cut(s)	756, 1000, 1227, 1820, 1994, 2115, 2337
BsmFI	GGGAC	6 cut(s)	41, 1562, 1682, 1688, 2203, 2306
BsmI	GAATGC	1 cut(s)	1617
BsnI	GGCC	7 cut(s)	71, 540, 864, 1021, 1164, 1590, 2302
BsoBI	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
Bsp1286I	GDGCHC	5 cut(s)	381, 504, 700, 1981, 2211
Bsp143I	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
Bsp1720I	GCTNAGC	1 cut(s)	1722
BspACI	CCGC	8 cut(s)	224, 337, 530, 1077, 1636, 1666, 1715, 2324
BspANI	GGCC	7 cut(s)	71, 540, 864, 1021, 1164, 1590, 2302
BspCNI	CTCAG	8 cut(s)	96, 851, 1409, 1714, 1894, 2122, 2125, 2274
BspFNI	CGCG	3 cut(s)	1668, 1743, 2151
BspHI	TCATGA	2 cut(s)	1197, 1458
BspMAI	CTGCAG	1 cut(s)	1756
BspOI	GCTAGC	1 cut(s)	1268
BspPI	GGATC	3 cut(s)	12, 1195, 2172
BspQI	GCTCTTC	1 cut(s)	2148
BspT107I	GGYRCC	2 cut(s)	635, 1424
BsrDI	GCAATG	2 cut(s)	852, 1209
BsrFI	RCCGGY	2 cut(s)	2008, 2071
BsrI	ACTGG	5 cut(s)	378, 665, 728, 1541, 1874
BssAI	RCCGGY	2 cut(s)	2008, 2071
BssECI	CCNNGG	7 cut(s)	82, 405, 558, 1246, 1308, 1579, 2296
BssMI	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
BssSI	CACGAG	1 cut(s)	1930
BssT1I	CCWWGG	3 cut(s)	82, 558, 1308
Bst2BI	CACGAG	1 cut(s)	1930
Bst2UI	CCWGG	5 cut(s)	406, 542, 1247, 1429, 1581
Bst4CI	ACNGT	7 cut(s)	291, 731, 1012, 1279, 1796, 1963, 2038
Bst6I	CTCTTC	5 cut(s)	168, 2077, 2148, 2243, 2340
BstAPI	GCANNNNNTGC	1 cut(s)	851
BstC8I	GCNNGC	6 cut(s)	312, 341, 345, 942, 1266, 1719
BstDEI	CTNAG	9 cut(s)	104, 838, 1396, 1672, 1722, 1902, 2112, 2130, 2282
BstEII	GGTNACC	2 cut(s)	98, 1288
BstF5I	GGATG	5 cut(s)	807, 1173, 1197, 1437, 1857
BstFNI	CGCG	3 cut(s)	1668, 1743, 2151
BstH2I	RGCGCY	1 cut(s)	2315
BstHHI	GCGC	3 cut(s)	137, 1450, 2314
BstKTI	GATC	7 cut(s)	7, 859, 984, 1185, 1203, 1606, 2167
BstMAI	GTCTC	7 cut(s)	756, 1000, 1227, 1820, 1994, 2115, 2337
BstMBI	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
BstMWI	GCNNNNNNNGC	7 cut(s)	353, 834, 851, 1043, 1723, 2183, 2192
BstNI	CCWGG	5 cut(s)	406, 542, 1247, 1429, 1581
BstNSI	RCATGY	2 cut(s)	766, 1299
BstPAI	GACNNNNGTC	1 cut(s)	1872
BstPI	GGTNACC	2 cut(s)	98, 1288
BstSCI	CCNGG	6 cut(s)	404, 540, 1245, 1427, 1579, 2295
BstSFI	CTRYAG	3 cut(s)	1752, 1768, 2037
BstSLI	GKGCMC	2 cut(s)	1981, 2211
BstUI	CGCG	3 cut(s)	1668, 1743, 2151
BstV1I	GCAGC	4 cut(s)	931, 970, 1766, 2186
BstV2I	GAAGAC	3 cut(s)	153, 193, 788
BstX2I	RGATCY	3 cut(s)	4, 1603, 2164
BstXI	CCANNNNNNTGG	1 cut(s)	83
BstYI	RGATCY	3 cut(s)	4, 1603, 2164
Bsu36I	CCTNAGG	1 cut(s)	2282
BsuI	GTATCC	2 cut(s)	157, 1784
BsuRI	GGCC	7 cut(s)	71, 540, 864, 1021, 1164, 1590, 2302
BtrI	CACGTC	1 cut(s)	2308
BtsCI	GGATG	5 cut(s)	807, 1173, 1197, 1437, 1857
BtsI	GCAGTG	3 cut(s)	444, 1221, 1275
BtsIMutI	CAGTG	8 cut(s)	287, 444, 672, 700, 721, 1221, 1275, 1899
Cac8I	GCNNGC	6 cut(s)	312, 341, 345, 942, 1266, 1719
CaiI	CAGNNNCTG	2 cut(s)	1010, 1700
CciI	TCATGA	2 cut(s)	1197, 1458
CfoI	GCGC	3 cut(s)	137, 1450, 2314
Cfr10I	RCCGGY	2 cut(s)	2008, 2071
Cfr13I	GGNCC	7 cut(s)	1020, 1553, 1576, 1748, 1764, 2300, 2320
CseI	GACGC	1 cut(s)	1657
Csp6I	GTAC	5 cut(s)	188, 279, 419, 1031, 1157
CspAI	ACCGGT	1 cut(s)	2071
CviQI	GTAC	5 cut(s)	188, 279, 419, 1031, 1157
DdeI	CTNAG	9 cut(s)	104, 838, 1396, 1672, 1722, 1902, 2112, 2130, 2282
DpnI	GATC	7 cut(s)	6, 858, 983, 1184, 1202, 1605, 2166
DpnII	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
DriI	GACNNNNNGTC	2 cut(s)	1949, 2107
EaeI	YGGCCR	2 cut(s)	862, 1162
Eam1104I	CTCTTC	5 cut(s)	168, 2077, 2148, 2243, 2340
Eam1105I	GACNNNNNGTC	2 cut(s)	1949, 2107
EarI	CTCTTC	5 cut(s)	168, 2077, 2148, 2243, 2340
Eco130I	CCWWGG	3 cut(s)	82, 558, 1308
Eco47I	GGWCC	5 cut(s)	1553, 1576, 1748, 1764, 2320
Eco57I	CTGAAG	1 cut(s)	2336
Eco81I	CCTNAGG	1 cut(s)	2282
Eco88I	CYCGRG	4 cut(s)	422, 1178, 1572, 2339
Eco91I	GGTNACC	2 cut(s)	98, 1288
EcoO109I	RGGNCCY	2 cut(s)	1576, 2320
EcoO65I	GGTNACC	2 cut(s)	98, 1288
EcoRII	CCWGG	5 cut(s)	404, 540, 1245, 1427, 1579
EcoT14I	CCWWGG	3 cut(s)	82, 558, 1308
ErhI	CCWWGG	3 cut(s)	82, 558, 1308
FalI	AAGNNNNNCTT	4 cut(s)	224, 256, 309, 341
FaqI	GGGAC	6 cut(s)	41, 1562, 1682, 1688, 2203, 2306
FauI	CCCGC	2 cut(s)	1643, 2331
FblI	GTMKAC	2 cut(s)	1922, 1942
Fnu4HI	GCNGC	5 cut(s)	945, 959, 1715, 1755, 2175
FokI	GGATG	5 cut(s)	814, 1160, 1204, 1424, 1844
Fsp4HI	GCNGC	5 cut(s)	945, 959, 1715, 1755, 2175
FspBI	CTAG	7 cut(s)	1034, 1265, 1544, 1592, 1835, 1890, 1946
GlaI	GCGC	3 cut(s)	136, 1449, 2313
GluI	GCNGC	5 cut(s)	945, 959, 1715, 1755, 2175
GsaI	CCCAGC	2 cut(s)	2128, 2190
GsuI	CTGGAG	2 cut(s)	395, 492
HaeII	RGCGCY	1 cut(s)	2315
HaeIII	GGCC	7 cut(s)	71, 540, 864, 1021, 1164, 1590, 2302
HapII	CCGG	5 cut(s)	1614, 2009, 2072, 2296, 2327
HgaI	GACGC	1 cut(s)	1657
HhaI	GCGC	3 cut(s)	137, 1450, 2314
Hin6I	GCGC	3 cut(s)	135, 1448, 2312
HinP1I	GCGC	3 cut(s)	135, 1448, 2312
HincII	GTYRAC	1 cut(s)	1816
HindII	GTYRAC	1 cut(s)	1816
HindIII	AAGCTT	1 cut(s)	1558
HpaII	CCGG	5 cut(s)	1614, 2009, 2072, 2296, 2327
HphI	GGTGA	9 cut(s)	110, 421, 503, 560, 956, 968, 1324, 1399, 1600
Hpy166II	GTNNAC	8 cut(s)	127, 188, 1141, 1599, 1816, 1923, 1943, 1979
Hpy188I	TCNGA	7 cut(s)	163, 650, 841, 1125, 1150, 1418, 1958
Hpy8I	GTNNAC	8 cut(s)	127, 188, 1141, 1599, 1816, 1923, 1943, 1979
Hpy99I	CGWCG	2 cut(s)	2105, 2242
HpyAV	CCTTC	6 cut(s)	34, 260, 335, 385, 1451, 2311
HpyCH4III	ACNGT	7 cut(s)	291, 731, 1012, 1279, 1796, 1963, 2038
HpyCH4IV	ACGT	3 cut(s)	2055, 2217, 2307
HpyF10VI	GCNNNNNNNGC	7 cut(s)	353, 834, 851, 1043, 1723, 2183, 2192
HpyF3I	CTNAG	9 cut(s)	104, 838, 1396, 1672, 1722, 1902, 2112, 2130, 2282
HpySE526I	ACGT	3 cut(s)	2055, 2217, 2307
HspAI	GCGC	3 cut(s)	135, 1448, 2312
KflI	GGGWCCC	1 cut(s)	1576
Kzo9I	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
LguI	GCTCTTC	1 cut(s)	2148
LmnI	GCTCC	4 cut(s)	376, 562, 1379, 1898
Lsp1109I	GCAGC	4 cut(s)	931, 970, 1766, 2186
LweI	GCATC	5 cut(s)	574, 699, 970, 1182, 1922
MaeI	CTAG	7 cut(s)	1034, 1265, 1544, 1592, 1835, 1890, 1946
MaeII	ACGT	3 cut(s)	2055, 2217, 2307
MaeIII	GTNAC	5 cut(s)	98, 974, 1216, 1288, 1642
MalI	GATC	7 cut(s)	6, 858, 983, 1184, 1202, 1605, 2166
MboI	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
MfeI	CAATTG	1 cut(s)	1186
MflI	RGATCY	3 cut(s)	4, 1603, 2164
MhlI	GDGCHC	5 cut(s)	381, 504, 700, 1981, 2211
MlsI	TGGCCA	2 cut(s)	864, 1164
MluCI	AATT	9 cut(s)	15, 456, 486, 513, 545, 588, 1186, 1382, 2290
MluNI	TGGCCA	2 cut(s)	864, 1164
MlyI	GAGTC	6 cut(s)	202, 1137, 1811, 1996, 2117, 2339
Mox20I	TGGCCA	2 cut(s)	864, 1164
MroXI	GAANNNNTTC	4 cut(s)	150, 1084, 1255, 2252
MscI	TGGCCA	2 cut(s)	864, 1164
MseI	TTAA	5 cut(s)	18, 1112, 2220, 2243, 2262
MslI	CAYNNNNRTG	3 cut(s)	573, 1214, 1809
Msp20I	TGGCCA	2 cut(s)	864, 1164
MspA1I	CMGCKG	2 cut(s)	226, 1077
MspI	CCGG	5 cut(s)	1614, 2009, 2072, 2296, 2327
MspR9I	CCNGG	6 cut(s)	406, 542, 1247, 1429, 1581, 2297
MunI	CAATTG	1 cut(s)	1186
Mva1269I	GAATGC	1 cut(s)	1617
MvaI	CCWGG	5 cut(s)	406, 542, 1247, 1429, 1581
MvnI	CGCG	3 cut(s)	1668, 1743, 2151
MwoI	GCNNNNNNNGC	7 cut(s)	353, 834, 851, 1043, 1723, 2183, 2192
NciI	CCSGG	1 cut(s)	2297
NdeII	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
NheI	GCTAGC	1 cut(s)	1264
NmuCI	GTSAC	3 cut(s)	98, 974, 1216
NspI	RCATGY	2 cut(s)	766, 1299
PaeR7I	CTCGAG	2 cut(s)	422, 2339
PagI	TCATGA	2 cut(s)	1197, 1458
PciI	ACATGT	1 cut(s)	762
PciSI	GCTCTTC	1 cut(s)	2148
PctI	GAATGC	1 cut(s)	1617
PdmI	GAANNNNTTC	4 cut(s)	150, 1084, 1255, 2252
PfeI	GAWTC	5 cut(s)	44, 645, 651, 1566, 2253
PinAI	ACCGGT	1 cut(s)	2071
PkrI	GCNGC	5 cut(s)	946, 960, 1716, 1756, 2176
PleI	GAGTC	6 cut(s)	202, 1136, 1811, 1996, 2116, 2338
PpsI	GAGTC	6 cut(s)	202, 1136, 1811, 1996, 2116, 2338
PpuMI	RGGWCCY	2 cut(s)	1576, 2320
PscI	ACATGT	1 cut(s)	762
PshAI	GACNNNNGTC	1 cut(s)	1872
PsiI	TTATAA	2 cut(s)	369, 2274
Psp5II	RGGWCCY	2 cut(s)	1576, 2320
Psp6I	CCWGG	5 cut(s)	404, 540, 1245, 1427, 1579
PspEI	GGTNACC	2 cut(s)	98, 1288
PspFI	CCCAGC	2 cut(s)	2124, 2186
PspGI	CCWGG	5 cut(s)	404, 540, 1245, 1427, 1579
PspPI	GGNCC	7 cut(s)	1020, 1553, 1576, 1748, 1764, 2300, 2320
PspPPI	RGGWCCY	2 cut(s)	1576, 2320
PsrI	GAACNNNNNNTAC	2 cut(s)	749, 781
PstI	CTGCAG	1 cut(s)	1756
PstNI	CAGNNNCTG	2 cut(s)	1010, 1700
PsuI	RGATCY	3 cut(s)	4, 1603, 2164
RsaI	GTAC	5 cut(s)	189, 280, 420, 1032, 1158
RsaNI	GTAC	5 cut(s)	188, 279, 419, 1031, 1157
RseI	CAYNNNNRTG	3 cut(s)	573, 1214, 1809
SapI	GCTCTTC	1 cut(s)	2148
SaqAI	TTAA	5 cut(s)	18, 1112, 2220, 2243, 2262
SatI	GCNGC	5 cut(s)	945, 959, 1715, 1755, 2175
Sau3AI	GATC	7 cut(s)	4, 856, 981, 1182, 1200, 1603, 2164
Sau96I	GGNCC	7 cut(s)	1020, 1553, 1576, 1748, 1764, 2300, 2320
SchI	GAGTC	6 cut(s)	202, 1137, 1811, 1996, 2117, 2339
ScrFI	CCNGG	6 cut(s)	406, 542, 1247, 1429, 1581, 2297
SduI	GDGCHC	5 cut(s)	381, 504, 700, 1981, 2211
SfaNI	GCATC	5 cut(s)	574, 699, 970, 1182, 1922
SfcI	CTRYAG	3 cut(s)	1752, 1768, 2037
Sfr274I	CTCGAG	2 cut(s)	422, 2339
SinI	GGWCC	5 cut(s)	1553, 1576, 1748, 1764, 2320
SlaI	CTCGAG	2 cut(s)	422, 2339
SmiMI	CAYNNNNRTG	3 cut(s)	573, 1214, 1809
SmlI	CTYRAG	6 cut(s)	8, 422, 775, 2159, 2178, 2339
SmoI	CTYRAG	6 cut(s)	8, 422, 775, 2159, 2178, 2339
SpeI	ACTAGT	1 cut(s)	1834
Sse9I	AATT	9 cut(s)	15, 456, 486, 513, 545, 588, 1186, 1382, 2290
SsiI	CCGC	8 cut(s)	224, 337, 530, 1077, 1636, 1666, 1715, 2324
SspI	AATATT	1 cut(s)	819
SspMI	CTAG	7 cut(s)	1034, 1265, 1544, 1592, 1835, 1890, 1946
StyD4I	CCNGG	6 cut(s)	404, 540, 1245, 1427, 1579, 2295
StyI	CCWWGG	3 cut(s)	82, 558, 1308
TaaI	ACNGT	7 cut(s)	291, 731, 1012, 1279, 1796, 1963, 2038
TaiI	ACGT	3 cut(s)	2058, 2220, 2310
TaqI	TCGA	7 cut(s)	253, 423, 643, 1876, 1916, 2100, 2340
TaqII	GACCGA	1 cut(s)	1752
TasI	AATT	9 cut(s)	15, 456, 486, 513, 545, 588, 1186, 1382, 2290
TatI	WGTACW	3 cut(s)	187, 418, 1030
TauI	GCSGC	1 cut(s)	1717
TfiI	GAWTC	5 cut(s)	44, 645, 651, 1566, 2253
Tru1I	TTAA	5 cut(s)	18, 1112, 2220, 2243, 2262
Tru9I	TTAA	5 cut(s)	18, 1112, 2220, 2243, 2262
TscAI	CASTG	8 cut(s)	294, 451, 672, 700, 728, 1221, 1275, 1906
TseFI	GTSAC	3 cut(s)	98, 974, 1216
TseI	GCWGC	4 cut(s)	944, 958, 1754, 2174
Tsp45I	GTSAC	3 cut(s)	98, 974, 1216
TspDTI	ATGAA	6 cut(s)	186, 589, 1518, 1518, 1846, 2261
TspGWI	ACGGA	1 cut(s)	744
TspRI	CASTG	8 cut(s)	294, 451, 672, 700, 728, 1221, 1275, 1906
VneI	GTGCAC	1 cut(s)	1977
VpaK11BI	GGWCC	5 cut(s)	1553, 1576, 1748, 1764, 2320
XceI	RCATGY	2 cut(s)	766, 1299
XhoI	CTCGAG	2 cut(s)	422, 2339
XmiI	GTMKAC	2 cut(s)	1922, 1942
XmnI	GAANNNNTTC	4 cut(s)	150, 1084, 1255, 2252
XspI	CTAG	7 cut(s)	1034, 1265, 1544, 1592, 1835, 1890, 1946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.