Rorug01G0369300

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002101
Physical Location & Seq
Forward (+)
48379575 .. 48383399
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0369300.1

Sequence Viewer

Length: 933 bp
ATGAAGGTCACCGTAGTTACTAGGAGTGGTAGAGAGCTTTTCAAGGGTGGCCTCGAGCTCAGTGACTCTGCCACTGTTGCTGATCTGCAGGAGGAGATCCATAAGCGATCCAAAAAGTTCTATCCTTCAAGGCAGCGGCTTACTCTTCCTGTCCAACCAGGATCAAAAGAGAAGGCAGTTGTCCTTGCCTCCAAGAAGAGTCTCAAAGATTATTACAGTGGAAACTCAGACAACTTAACTGTGGTGTTCAAGGACTTAGGCCCACAAGTTGAATACCGAACGCTCTTCTTCTTTGAGTATATTGGTCCTTTGCTCAGTTATCCCATATTTTACTTCTTCCCTGTTTACCAGTACTTGGGCTTCAAAGGAGACCGTGTCATCCACCCAGTCCAGACATATGCTCTATATTATTATTGCTTCCACTACTTTAAACGTATTATGGAAACATTTTTCATACATAGGTTCAGCCATGCAACCTCACCTCTCTCCTATGTATCCCGGAATTGTGCTTATTATTGGAGCTTTGCAGCACTCATGTCTTACTACGTGAATCACCCACTTTACACCCCTGTTAGCGACCTCCAAATGAAGATTGGCTTTGGTTTTGGGATAATATGTCAAATCGCGAACTTGTATTGTCATATTCTATTGAGGAATCTACGAAGCCCTGATGGAAGTGGAGGCTATCAAATACCACGTGGTTTTCTATTCAATATTGTGACATGCGCAAATTATACAACTGAGATTTATCAGTGGTTGGGATTTAATATTGCAACGCAGACGGTTGCTGGTTATCTTTTTGCTGTAGTTGCTGCTTCTATCATGACCAATTGGGCCCTCGGAAAGCACCGCCGTTTAAAGAAGCTATTTGATGGGAAGGACGGAAGACCTAAGTATCCACGTCGATGGGTGATTCTACCGCCATTCTTGTAG

Protein Analysis

310

Amino Acids

35.93

Weight (kDa)

9.61

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 159 - 309 5.4e-26 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 727
AccB7I CCANNNNNTGG 1 cut(s) 355
AccII CGCG 1 cut(s) 626
AciI CCGC 3 cut(s) 136, 850, 920
AclWI GGATC 3 cut(s) 91, 102, 169
AcvI CACGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 6 cut(s) 43, 129, 250, 272, 364, 712
AjiI CACGTC 1 cut(s) 902
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 4 cut(s) 37, 58, 522, 865
AluI AGCT 4 cut(s) 37, 58, 522, 865
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 206, 363
AlwI GGATC 3 cut(s) 91, 102, 169
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 3 cut(s) 49, 259, 834
ApaI GGGCCC 1 cut(s) 838
ApeKI GCWGC 3 cut(s) 133, 527, 812
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 4 cut(s) 260, 305, 834, 835
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 3 cut(s) 471, 545, 922
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 838
BaeI ACNNNNGTAYC 2 cut(s) 878, 911
BanII GRGCYC 2 cut(s) 60, 838
BarI GAAGNNNNNNTAC 2 cut(s) 344, 376
BbrPI CACGTG 1 cut(s) 698
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 145, 539, 799
BccI CCATC 3 cut(s) 665, 866, 900
BceAI ACGGC 1 cut(s) 837
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 2 cut(s) 505, 906
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 206, 363
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 2 cut(s) 86, 804
BfuI GTATCC 2 cut(s) 505, 906
BisI GCNGC 4 cut(s) 134, 137, 528, 813
BlsI GCNGC 4 cut(s) 135, 138, 529, 814
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 159, 499
Bme18I GGWCC 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 902
BmgT120I GGNCC 4 cut(s) 260, 305, 834, 835
BmiI GGNNCC 1 cut(s) 836
BmrFI CCNGG 2 cut(s) 159, 499
BmrI ACTGGG 1 cut(s) 380
BmuI ACTGGG 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 892
BpuMI CCSGG 1 cut(s) 499
BsaAI YACGTR 2 cut(s) 547, 698
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 838
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 349, 386
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 838
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 4 cut(s) 73, 240, 328, 732
BseNI ACTGG 2 cut(s) 349, 386
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 3 cut(s) 145, 539, 799
Bsh1236I CGCG 1 cut(s) 626
BshFI GGCC 3 cut(s) 51, 261, 836
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 499
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 2 cut(s) 206, 363
BsnI GGCC 3 cut(s) 51, 261, 836
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 53
Bsp120I GGGCCC 1 cut(s) 834
Bsp1286I GDGCHC 2 cut(s) 60, 838
Bsp143I GATC 4 cut(s) 82, 96, 107, 161
Bsp68I TCGCGA 1 cut(s) 626
BspACI CCGC 3 cut(s) 136, 850, 920
BspANI GGCC 3 cut(s) 51, 261, 836
BspCNI CTCAG 4 cut(s) 72, 239, 327, 733
BspFNI CGCG 1 cut(s) 626
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 1 cut(s) 836
BspMAI CTGCAG 1 cut(s) 90
BspPI GGATC 3 cut(s) 91, 102, 169
BspQI GCTCTTC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 363
BsrI ACTGG 2 cut(s) 349, 386
BssECI CCNNGG 1 cut(s) 838
BssMI GATC 4 cut(s) 82, 96, 107, 161
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 6 cut(s) 13, 76, 218, 241, 374, 784
Bst6I CTCTTC 3 cut(s) 150, 191, 290
BstBAI YACGTR 2 cut(s) 547, 698
BstDEI CTNAG 6 cut(s) 59, 226, 256, 314, 741, 891
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 378
BstFNI CGCG 1 cut(s) 626
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 4 cut(s) 85, 99, 110, 164
BstMAI GTCTC 2 cut(s) 206, 363
BstMBI GATC 4 cut(s) 82, 96, 107, 161
BstMWI GCNNNNNNNGC 2 cut(s) 77, 809
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 726
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 157, 497
BstSFI CTRYAG 2 cut(s) 86, 804
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 3 cut(s) 145, 539, 799
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 906
BstYI RGATCY 1 cut(s) 96
BsuI GTATCC 2 cut(s) 505, 906
BsuRI GGCC 3 cut(s) 51, 261, 836
BtrI CACGTC 1 cut(s) 902
BtsCI GGATG 1 cut(s) 378
BtsIMutI CAGTG 4 cut(s) 67, 72, 223, 758
BtuMI TCGCGA 1 cut(s) 626
CciI TCATGA 1 cut(s) 822
CfoI GCGC 1 cut(s) 728
Cfr13I GGNCC 4 cut(s) 260, 305, 834, 835
Csp6I GTAC 1 cut(s) 352
CviAII CATG 4 cut(s) 470, 535, 723, 823
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 6 cut(s) 59, 226, 256, 314, 741, 891
DpnI GATC 4 cut(s) 84, 98, 109, 163
DpnII GATC 4 cut(s) 82, 96, 107, 161
DraI TTTAAA 2 cut(s) 430, 858
Eam1104I CTCTTC 3 cut(s) 150, 191, 290
EarI CTCTTC 3 cut(s) 150, 191, 290
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 60, 838
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 58
Eco72I CACGTG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 53
Eco91I GGTNACC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 835
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 157
EcoT38I GRGCYC 2 cut(s) 60, 838
FaeI CATG 4 cut(s) 473, 538, 726, 826
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FatI CATG 4 cut(s) 469, 534, 722, 822
FauNDI CATATG 1 cut(s) 397
Fnu4HI GCNGC 4 cut(s) 134, 137, 528, 813
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 2 cut(s) 60, 838
Fsp4HI GCNGC 4 cut(s) 134, 137, 528, 813
FspBI CTAG 1 cut(s) 21
FspI TGCGCA 1 cut(s) 727
GlaI GCGC 1 cut(s) 727
GluI GCNGC 4 cut(s) 134, 137, 528, 813
HaeIII GGCC 3 cut(s) 51, 261, 836
HapII CCGG 1 cut(s) 499
HhaI GCGC 1 cut(s) 728
Hin1II CATG 4 cut(s) 473, 538, 726, 826
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HinfI GANTC 5 cut(s) 65, 199, 550, 655, 913
HpaII CCGG 1 cut(s) 499
HphI GGTGA 3 cut(s) 471, 545, 922
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 229, 842
Hpy188III TCNNGA 3 cut(s) 391, 625, 823
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 906
HpyAV CCTTC 3 cut(s) 135, 166, 871
HpyCH4III ACNGT 6 cut(s) 13, 76, 218, 241, 374, 784
HpyCH4IV ACGT 4 cut(s) 433, 546, 697, 901
HpyCH4V TGCA 4 cut(s) 88, 473, 527, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 809
HpyF3I CTNAG 6 cut(s) 59, 226, 256, 314, 741, 891
HpySE526I ACGT 4 cut(s) 433, 546, 697, 901
Hsp92II CATG 4 cut(s) 473, 538, 726, 826
HspAI GCGC 1 cut(s) 726
Kzo9I GATC 4 cut(s) 82, 96, 107, 161
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 3 cut(s) 145, 539, 799
MaeI CTAG 1 cut(s) 21
MaeII ACGT 4 cut(s) 433, 546, 697, 901
MaeIII GTNAC 4 cut(s) 7, 16, 62, 718
MalI GATC 4 cut(s) 84, 98, 109, 163
MboI GATC 4 cut(s) 82, 96, 107, 161
MboII GAAGA 7 cut(s) 137, 208, 277, 280, 328, 601, 897
MfeI CAATTG 1 cut(s) 829
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 2 cut(s) 60, 838
MluCI AATT 3 cut(s) 502, 730, 829
MlyI GAGTC 2 cut(s) 59, 208
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 9 cut(s) 62, 85, 199, 487, 492, 590, 645, 674, 848
MseI TTAA 4 cut(s) 236, 429, 765, 857
MslI CAYNNNNRTG 1 cut(s) 904
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 2 cut(s) 159, 499
MunI CAATTG 1 cut(s) 829
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 2 cut(s) 77, 809
NciI CCSGG 1 cut(s) 499
NdeI CATATG 1 cut(s) 397
NdeII GATC 4 cut(s) 82, 96, 107, 161
NlaIII CATG 4 cut(s) 473, 538, 726, 826
NlaIV GGNNCC 1 cut(s) 836
NmuCI GTSAC 3 cut(s) 7, 62, 718
NruI TCGCGA 1 cut(s) 626
NsbI TGCGCA 1 cut(s) 727
NspI RCATGY 1 cut(s) 726
PaeR7I CTCGAG 1 cut(s) 53
PagI TCATGA 1 cut(s) 822
PciSI GCTCTTC 1 cut(s) 290
PfeI GAWTC 3 cut(s) 550, 655, 913
PflFI GACNNNGTC 1 cut(s) 374
PflMI CCANNNNNTGG 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 497
PkrI GCNGC 4 cut(s) 135, 138, 529, 814
PleI GAGTC 2 cut(s) 59, 207
PmaCI CACGTG 1 cut(s) 698
PmlI CACGTG 1 cut(s) 698
PpsI GAGTC 2 cut(s) 59, 207
Ppu21I YACGTR 2 cut(s) 547, 698
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 157
PspCI CACGTG 1 cut(s) 698
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 836
PspOMI GGGCCC 1 cut(s) 834
PspPI GGNCC 4 cut(s) 260, 305, 834, 835
PspXI VCTCGAGB 1 cut(s) 53
PstI CTGCAG 1 cut(s) 90
PsuI RGATCY 1 cut(s) 96
PsyI GACNNNGTC 1 cut(s) 374
RruI TCGCGA 1 cut(s) 626
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 904
SacI GAGCTC 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 4 cut(s) 236, 429, 765, 857
SatI GCNGC 4 cut(s) 134, 137, 528, 813
Sau3AI GATC 4 cut(s) 82, 96, 107, 161
Sau96I GGNCC 4 cut(s) 260, 305, 834, 835
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 59, 208
ScrFI CCNGG 2 cut(s) 159, 499
SduI GDGCHC 2 cut(s) 60, 838
SfcI CTRYAG 2 cut(s) 86, 804
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 305
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 904
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 3 cut(s) 502, 730, 829
SsiI CCGC 3 cut(s) 136, 850, 920
SspI AATATT 2 cut(s) 715, 769
SspMI CTAG 1 cut(s) 21
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 157, 497
TaaI ACNGT 6 cut(s) 13, 76, 218, 241, 374, 784
TaiI ACGT 4 cut(s) 436, 549, 700, 904
TaqI TCGA 2 cut(s) 54, 904
TasI AATT 3 cut(s) 502, 730, 829
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 3 cut(s) 550, 655, 913
Tru1I TTAA 4 cut(s) 236, 429, 765, 857
Tru9I TTAA 4 cut(s) 236, 429, 765, 857
TscAI CASTG 4 cut(s) 67, 79, 223, 758
TseFI GTSAC 3 cut(s) 7, 62, 718
TseI GCWGC 3 cut(s) 133, 527, 812
Tsp45I GTSAC 3 cut(s) 7, 62, 718
TspDTI ATGAA 3 cut(s) 17, 442, 602
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 4 cut(s) 67, 79, 223, 758
Tth111I GACNNNGTC 1 cut(s) 374
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 305
XceI RCATGY 1 cut(s) 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 1 cut(s) 21
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.