pycom01g17950

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002101
Physical Location & Seq
Forward (+)
17107476 .. 17109576
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17950.1

Sequence Viewer

Length: 1023 bp
ATGGATCTTGAGAGAATTAAGTGTTGGGACACTTCAAAGAAGGATTCTTGGAAGAATGTTATGCTGTTGGCCTACCAGAGCCTTGGAGTAGTGTATGGTGACCTGAGCATTTCCCCTCTCTATGTTTACAAGAGTGCATTTGCGGATGACATTCAACATTCGGATACGAATGAAGAGATTTATGGTGTACTTTCTTTTGTCTTCTGGACTCTTACTTTAGTCCCGCTATTCAAGTATGTCTTTATAGTCCTTCGAGCTGATGATAATGGAGAGGGCGGTACATTTGCACTGTACTCGTTGATATGTAGGCATGCGAAAGTAAGCCTTCTGCCCAACCGGCAGCTTGCTGATGAGGCACTTTCTACTTATAAATTGGAGCACCCACCGGAGAAGGAAAAAAGTTCGAGGGTGAAATTCGTACTTGAGAAGTGCAAGACCTTGCACACTGCTTTGCTAATTCTTGTTCTTCTTGGAACTTGTATGGTAATTGGCGATGGAGTGCTCACTCCAGCCATTTCAGTCTTTTCTGCGGTGTCTGGCCTTGAATTATCCATGTCCAAGGAACATCACCAGTATGCTGTGGTTCCAATTACTTGTTTCATATTGTTATGTCTATTTGCACTTCAACACTATGGCACCCATCGAATCGGATTCTTTTTTGCACCAGTGGTGTTGGCATGGCTTCTGTGCATCAGTGCTCTTGGCTTGTATAATATAATTCACTGGAACCAGCATGTCTATCAAGCTCTGTCTCCGTATTACATGTTCAAGTTCTTGAGGAAGACAAGGATAAGTGGGTGGATGTCTCTAGGTGGAATATTGCTGTGCATAACAGGCTCAGAGGCAATGTTTGCTAAGGGAGGGATCTTGGCCACTTCTCATATAATGCCATTCAGGTTGCTTTTACCTTTTTGGTTTATCCAGCGCTTATATTGGCATATATGGGGCAGGCTGCTTACTTATCGCAACATCACCACACCAGTCACCGGATCAGTTTCTATGTCTCGGTTCCAGAAAGTGTGA

Protein Analysis

341

Amino Acids

38.69

Weight (kDa)

8.99

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 23 - 295 6.5e-86 K+ potassium transporter integral membrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 635
AciI CCGC 4 cut(s) 143, 224, 276, 530
AclWI GGATC 3 cut(s) 12, 872, 997
AcoI YGGCCR 1 cut(s) 870
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 4 cut(s) 189, 280, 293, 420
AfeI AGCGCT 1 cut(s) 926
AfiI CCNNNNNNNGG 1 cut(s) 986
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 6 cut(s) 36, 155, 232, 545, 626, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 7, 39
AluBI AGCT 3 cut(s) 257, 343, 746
AluI AGCT 3 cut(s) 257, 343, 746
Alw21I GWGCWC 3 cut(s) 381, 504, 700
Alw26I GTCTC 3 cut(s) 756, 810, 1008
AlwI GGATC 3 cut(s) 12, 872, 997
Aor51HI AGCGCT 1 cut(s) 926
AoxI GGCC 3 cut(s) 69, 538, 870
ApeKI GCWGC 2 cut(s) 340, 952
ApoI RAATTY 1 cut(s) 413
AspLEI GCGC 1 cut(s) 927
AsuHPI GGTGA 5 cut(s) 110, 421, 560, 964, 976
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 1 cut(s) 635
BbsI GAAGAC 2 cut(s) 193, 788
Bbv12I GWGCWC 3 cut(s) 381, 504, 700
BbvI GCAGC 2 cut(s) 352, 939
BccI CCATC 2 cut(s) 488, 648
BcgI CGANNNNNNTGC 2 cut(s) 276, 310
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 3 cut(s) 756, 810, 1008
BfaI CTAG 1 cut(s) 809
BfoI RGCGCY 1 cut(s) 928
BfuI GTATCC 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 337
BisI GCNGC 2 cut(s) 341, 953
BlsI GCNGC 2 cut(s) 342, 954
BmiI GGNNCC 4 cut(s) 585, 637, 728, 1010
BmsI GCATC 1 cut(s) 699
BpiI GAAGAC 2 cut(s) 193, 788
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 2 cut(s) 104, 855
BpuEI CTTGAG 3 cut(s) 29, 443, 796
BsaJI CCNNGG 2 cut(s) 82, 558
BsaWI WCCGGW 2 cut(s) 385, 986
Bsc4I CCNNNNNNNGG 1 cut(s) 986
Bse118I RCCGGY 1 cut(s) 336
Bse1I ACTGG 4 cut(s) 571, 665, 728, 980
Bse3DI GCAATG 1 cut(s) 852
BseDI CCNNGG 2 cut(s) 82, 558
BseGI GGATG 2 cut(s) 151, 807
BseLI CCNNNNNNNGG 1 cut(s) 986
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 95, 852
BseNI ACTGG 4 cut(s) 571, 665, 728, 980
BseXI GCAGC 2 cut(s) 352, 939
BshFI GGCC 3 cut(s) 71, 540, 872
BshNI GGYRCC 1 cut(s) 635
BsiHKAI GWGCWC 3 cut(s) 381, 504, 700
BsiSI CCGG 3 cut(s) 337, 386, 987
BslFI GGGAC 2 cut(s) 41, 206
BslI CCNNNNNNNGG 1 cut(s) 986
BsmAI GTCTC 3 cut(s) 756, 810, 1008
BsmFI GGGAC 2 cut(s) 41, 206
BsnI GGCC 3 cut(s) 71, 540, 872
Bsp1286I GDGCHC 3 cut(s) 381, 504, 700
Bsp143I GATC 3 cut(s) 4, 864, 989
BspACI CCGC 4 cut(s) 143, 224, 276, 530
BspANI GGCC 3 cut(s) 71, 540, 872
BspCNI CTCAG 2 cut(s) 96, 851
BspLI GGNNCC 4 cut(s) 585, 637, 728, 1010
BspPI GGATC 3 cut(s) 12, 872, 997
BspT107I GGYRCC 1 cut(s) 635
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 1 cut(s) 336
BsrI ACTGG 4 cut(s) 571, 665, 728, 980
BssAI RCCGGY 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 82, 558
BssMI GATC 3 cut(s) 4, 864, 989
BssT1I CCWWGG 2 cut(s) 82, 558
Bst4CI ACNGT 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 3 cut(s) 312, 345, 950
BstDEI CTNAG 3 cut(s) 104, 838, 855
BstEII GGTNACC 1 cut(s) 98
BstF5I GGATG 2 cut(s) 151, 807
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 1 cut(s) 927
BstKTI GATC 3 cut(s) 7, 867, 992
BstMAI GTCTC 3 cut(s) 756, 810, 1008
BstMBI GATC 3 cut(s) 4, 864, 989
BstMWI GCNNNNNNNGC 4 cut(s) 337, 353, 834, 851
BstNSI RCATGY 3 cut(s) 314, 737, 766
BstPI GGTNACC 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 352, 939
BstV2I GAAGAC 2 cut(s) 193, 788
BstX2I RGATCY 2 cut(s) 4, 864
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 2 cut(s) 4, 864
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 3 cut(s) 71, 540, 872
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 2 cut(s) 151, 807
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 5 cut(s) 287, 444, 672, 700, 721
Cac8I GCNNGC 3 cut(s) 312, 345, 950
CfoI GCGC 1 cut(s) 927
Cfr10I RCCGGY 1 cut(s) 336
Csp6I GTAC 4 cut(s) 188, 279, 292, 419
CviAII CATG 5 cut(s) 311, 553, 678, 734, 763
CviQI GTAC 4 cut(s) 188, 279, 292, 419
DdeI CTNAG 3 cut(s) 104, 838, 855
DpnI GATC 3 cut(s) 6, 866, 991
DpnII GATC 3 cut(s) 4, 864, 989
EaeI YGGCCR 1 cut(s) 870
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 82, 558
Eco47III AGCGCT 1 cut(s) 926
Eco91I GGTNACC 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 98
EcoT14I CCWWGG 2 cut(s) 82, 558
ErhI CCWWGG 2 cut(s) 82, 558
FaeI CATG 5 cut(s) 314, 556, 681, 737, 766
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 309, 341
FaqI GGGAC 2 cut(s) 41, 206
FatI CATG 5 cut(s) 310, 552, 677, 733, 762
FauI CCCGC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 341, 953
FokI GGATG 2 cut(s) 158, 814
Fsp4HI GCNGC 2 cut(s) 341, 953
FspBI CTAG 1 cut(s) 809
GlaI GCGC 1 cut(s) 926
GluI GCNGC 2 cut(s) 341, 953
GsuI CTGGAG 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 928
HaeIII GGCC 3 cut(s) 71, 540, 872
HapII CCGG 3 cut(s) 337, 386, 987
HhaI GCGC 1 cut(s) 927
Hin1II CATG 5 cut(s) 314, 556, 681, 737, 766
Hin6I GCGC 1 cut(s) 925
HinP1I GCGC 1 cut(s) 925
HinfI GANTC 4 cut(s) 44, 208, 645, 651
HpaII CCGG 3 cut(s) 337, 386, 987
HphI GGTGA 5 cut(s) 110, 421, 560, 964, 976
Hpy166II GTNNAC 2 cut(s) 127, 188
Hpy188I TCNGA 3 cut(s) 163, 650, 841
Hpy188III TCNNGA 4 cut(s) 8, 205, 775, 1012
Hpy8I GTNNAC 2 cut(s) 127, 188
HpyAV CCTTC 4 cut(s) 34, 260, 335, 385
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4V TGCA 8 cut(s) 137, 287, 432, 442, 620, 662, 690, 828
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 353, 834, 851
HpyF3I CTNAG 3 cut(s) 104, 838, 855
Hsp92II CATG 5 cut(s) 314, 556, 681, 737, 766
HspAI GCGC 1 cut(s) 925
Kzo9I GATC 3 cut(s) 4, 864, 989
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 2 cut(s) 352, 939
LweI GCATC 1 cut(s) 699
MaeI CTAG 1 cut(s) 809
MaeIII GTNAC 2 cut(s) 98, 982
MalI GATC 3 cut(s) 6, 866, 991
MboI GATC 3 cut(s) 4, 864, 989
MboII GAAGA 5 cut(s) 64, 185, 193, 458, 793
MflI RGATCY 2 cut(s) 4, 864
MhlI GDGCHC 3 cut(s) 381, 504, 700
MlsI TGGCCA 1 cut(s) 872
MluCI AATT 8 cut(s) 15, 371, 413, 456, 486, 545, 588, 717
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 7 cut(s) 126, 265, 346, 399, 771, 835, 854
Mox20I TGGCCA 1 cut(s) 872
MscI TGGCCA 1 cut(s) 872
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 573
Msp20I TGGCCA 1 cut(s) 872
MspI CCGG 3 cut(s) 337, 386, 987
MwoI GCNNNNNNNGC 4 cut(s) 337, 353, 834, 851
NdeII GATC 3 cut(s) 4, 864, 989
NlaIII CATG 5 cut(s) 314, 556, 681, 737, 766
NlaIV GGNNCC 4 cut(s) 585, 637, 728, 1010
NmuCI GTSAC 2 cut(s) 98, 982
NspI RCATGY 3 cut(s) 314, 737, 766
PaeI GCATGC 1 cut(s) 314
PciI ACATGT 1 cut(s) 762
PfeI GAWTC 3 cut(s) 44, 645, 651
PkrI GCNGC 2 cut(s) 342, 954
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PscI ACATGT 1 cut(s) 762
PsiI TTATAA 1 cut(s) 369
PspEI GGTNACC 1 cut(s) 98
PspN4I GGNNCC 4 cut(s) 585, 637, 728, 1010
PsrI GAACNNNNNNTAC 2 cut(s) 749, 781
PsuI RGATCY 2 cut(s) 4, 864
RsaI GTAC 4 cut(s) 189, 280, 293, 420
RsaNI GTAC 4 cut(s) 188, 279, 292, 419
RseI CAYNNNNRTG 1 cut(s) 573
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 2 cut(s) 341, 953
Sau3AI GATC 3 cut(s) 4, 864, 989
SchI GAGTC 1 cut(s) 202
SduI GDGCHC 3 cut(s) 381, 504, 700
SetI ASST 8 cut(s) 105, 259, 345, 440, 748, 814, 899, 910
SfaNI GCATC 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 3 cut(s) 8, 422, 775
SmoI CTYRAG 3 cut(s) 8, 422, 775
SphI GCATGC 1 cut(s) 314
Sse9I AATT 8 cut(s) 15, 371, 413, 456, 486, 545, 588, 717
SsiI CCGC 4 cut(s) 143, 224, 276, 530
SspI AATATT 1 cut(s) 819
SspMI CTAG 1 cut(s) 809
StyI CCWWGG 2 cut(s) 82, 558
TaaI ACNGT 1 cut(s) 291
TaqI TCGA 3 cut(s) 253, 404, 643
TasI AATT 8 cut(s) 15, 371, 413, 456, 486, 545, 588, 717
TatI WGTACW 2 cut(s) 187, 291
TfiI GAWTC 3 cut(s) 44, 645, 651
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 5 cut(s) 294, 451, 672, 700, 728
TseFI GTSAC 2 cut(s) 98, 982
TseI GCWGC 2 cut(s) 340, 952
Tsp45I GTSAC 2 cut(s) 98, 982
TspDTI ATGAA 2 cut(s) 186, 589
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 5 cut(s) 294, 451, 672, 700, 728
XapI RAATTY 1 cut(s) 413
XceI RCATGY 3 cut(s) 314, 737, 766
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.