Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013719

Physical Location & Seq

Genomic Coordinates Forward (+)

32184709 .. 32188974

Gene Structure 4266 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1254500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGACACCACCAACAACACTTCCTACACTCCCTTGCCGGGGGAAGACCCCTCCGCCGCCGTCCCTCCGCCGACCCATCGGAGACCCTTTAAGGGTTTTGCCGTGATATTCTCCTCCGTCATATTTCTCTTGTCATTTGTCACATTAGTTATCCACCAAGGACCGGGGGCGCCGCCAAAAACAGTGCCAGAACAACCGGATCACCACCACCAATACAGGCCAGCTTCAACCAGTTCAGAGACGAGGTCGTTTTCAGTGCCTCGAGGAAAATTAGAAGGAGTATCGGCCAAATCGTACCCTCATTTTTCAGAGGAAGCTTCATATAATTGGACAAATGCCATGTTTTCCTGGCAAAGAACTGCATTCCATTTCCAGCCAGAAAAAAATTGGATAAATGATCCGAATGGGCCGTTGTTTCACAAGGGATGGTATCACCTGTTCTACCAGTACAACCCGGACTCAGCTGTGTGGGGCAACATCACATGGGGCCATGCGGTATCAACGGACATGATTCACTGGCTCTACCTCCCGCTCGCCATGGTCCCCGATCGATGGTTCGATGCGAACGGAGTGTGGACCGGGTCCGCCACCCTCCTCCCTGACGGCCAAATCATAATGTTGTACACTGGCAGCACCAATGATTCCGTGCAAGTACAAAACCTCGCCTACCCTGCCAACCTATCGGATCCCCTTTTACTTGATTGGGTCAAGTACGAAGGTAATCCGATTTTAACTCCGCCATCCGGGATCGGATCCACTGATTTCCGTGACCCCACAACCGCGTGGATTGGTCCCGATGGCAAATGGCGAACTACCATTGGATCAAAATTCAACAAAACTGGAATTTCAATGGTGTACACCACCACTGATTTTATAAACTATGAGCTCCATGATGGGGTCTTGCATGAGGTCCCGGGTACGGGTATGTGGGAGTGCGTGGACTTTTACCCGGTTGCTATAAACGGGTCCAAGGGTTTGGACACGTCGGTAAACAACGGCGGAGTTAAGCACGTGCTGAAGGCTAGTTTGGATGACACAAAATTGGATCATTACGCAATTGGGACTTATTTTATAGAGAATGAGACATGGGTACCCGATGACCCGACTATCGACGTTGGTATCGGGTTGAAGTATGACTATGGAAGGTACTATGCATCAAAGACATTTTATGATCAAAATAAGGAGAGAAGGATCTTGTTGGGTTGGATTAATGAAACTGACACTGAGACTGATGATTTGAAGAAACGTTGGGCTTCCCTTCACACCATTCCGAGGACGGTGTTGTTTGATAGCAAAACTGGAACCAATTTGCTTCAATGGCCGGTGGAGGAGATTGAAGACTTGAGATTAAACAGTACCGAATTTACTGATGTTTTGGTTGAAGCAGGAACAGTTGTGCCACTTGATATTGGAACTGCCACACAGTTGGACATATTCGTCGACTTTGAAATCGAGTTATTGGACACAGAGGAGAGCGTGAATGGGAGTTCAGGGTGTGGTGATGGTGCTGTAGATAGGAGCACATTCGGACCCTTTGGCATTTTGGTCATTGCAGATGAATCTCTTTCCGAGTTAACCCCGGTTTATTTTCGAGTTACCAACTCGACCGATGGGGACGTCACGACTTACTTCTGCGCCGACGAATCGAGGTCCTCCAAGGCTCCTGAAGTCTTCAAACAAGTTTATGGAGGCGAAGTTCCTGTGTTGGATGGTGAAACTTATTCTGCCAGAGTGTTGGTTGATCATTCGATCGTGGAAAGCTACGCGCAAGGGGGCAGGACGGTAATTTCATCGCGGGTTTACCCGACAGAAGCAATCTATGGAGCAGCAAGGCTGTTCTTGTTCAACAATGCAACAGGAGTGAATGTCAAGGCTACCCTCAAGATTTGGCAGCTCAACTCAGCCTTCATTCATCCTTTCCCATTAGACCAGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

646

Amino Acids

71.88

Weight (kDa)

4.83

Isoelectric Point (pI)

32.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	8 - 114	3.4e-16	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	123 - 441	3.7e-100	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	444 - 631	1.8e-21	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013719)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g27630 FvH4_7g27630 FvH4_7g27630
malus_domestica	MD07G1254500.v1.1 MD11G1195800.v1.1
prunus_persica	Prupe.2G277900_v2.0.a1 Prupe.2G277900_v2.0.a1 Prupe.2G277900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0374511
rosa_laevigata	RLG00000026733
rosa_multiflora	Rmu_ssc0000007.1_g000003
rosa_roxburghii	Rroxscaffold_4G00283440
rosa_rugosa	Rorug01G0383900
rosa_samantha	Rh1AG394400 Rh1BG359000 Rh1CG372100 Rh1DG389600
rosa_wichuraiana	Rw1G034170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	875
AasI	GACNNNNNNGTC	1 cut(s)	1436
AatII	GACGTC	1 cut(s)	1620
AbsI	CCTCGAGG	1 cut(s)	261
Acc65I	GGTACC	1 cut(s)	1090
AccB1I	GGYRCC	2 cut(s)	169, 1090
AccBSI	CCGCTC	1 cut(s)	532
AccI	GTMKAC	1 cut(s)	1440
AccII	CGCG	3 cut(s)	782, 1766, 1795
AclI	AACGTT	1 cut(s)	1246
AcoI	YGGCCR	3 cut(s)	285, 604, 1319
AcsI	RAATTY	3 cut(s)	827, 843, 1361
AcuI	CTGAAG	2 cut(s)	1037, 1686
AcvI	CACGTG	1 cut(s)	1012
AcyI	GRCGYC	2 cut(s)	170, 1617
AflIII	ACRYGT	1 cut(s)	981
AjiI	CACGTC	1 cut(s)	984
AjnI	CCWGG	1 cut(s)	347
AjuI	GAANNNNNNNTTGG	2 cut(s)	1010, 1042
AloI	GAACNNNNNNTCC	2 cut(s)	1680, 1712
AluBI	AGCT	6 cut(s)	224, 317, 464, 886, 1761, 1894
AluI	AGCT	6 cut(s)	224, 317, 464, 886, 1761, 1894
Alw21I	GWGCWC	2 cut(s)	888, 1523
Alw26I	GTCTC	4 cut(s)	76, 233, 1076, 1220
Ama87I	CYCGRG	2 cut(s)	261, 913
AoxI	GGCC	6 cut(s)	218, 285, 407, 487, 604, 1319
ApeKI	GCWGC	3 cut(s)	630, 1826, 1891
ApoI	RAATTY	3 cut(s)	827, 843, 1361
ArsI	GACNNNNNNTTYG	2 cut(s)	1219, 1251
AseI	ATTAAT	1 cut(s)	1209
Asp718I	GGTACC	1 cut(s)	1090
AspLEI	GCGC	3 cut(s)	172, 1637, 1768
AsuC2I	CCSGG	9 cut(s)	39, 165, 455, 580, 745, 914, 915, 950, 1580
AsuHPI	GGTGA	4 cut(s)	194, 425, 1511, 1724
AvaI	CYCGRG	2 cut(s)	261, 913
AvaII	GGWCC	9 cut(s)	161, 541, 576, 582, 791, 910, 966, 1529, 1650
BaeI	ACNNNNGTAYC	4 cut(s)	1074, 1102, 1107, 1135
BamHI	GGATCC	2 cut(s)	685, 752
BanI	GGYRCC	2 cut(s)	169, 1090
BanII	GRGCYC	1 cut(s)	888
BbrPI	CACGTG	1 cut(s)	1012
BbsI	GAAGAC	3 cut(s)	51, 1344, 1663
Bbv12I	GWGCWC	2 cut(s)	888, 1523
BbvI	GCAGC	3 cut(s)	642, 1838, 1903
BccI	CCATC	9 cut(s)	84, 420, 546, 748, 791, 887, 1496, 1604, 1703
BceAI	ACGGC	5 cut(s)	44, 86, 394, 619, 1012
BciT130I	CCWGG	1 cut(s)	349
BclI	TGATCA	2 cut(s)	1171, 1741
BcnI	CCSGG	9 cut(s)	39, 165, 455, 580, 745, 914, 915, 950, 1580
BcoDI	GTCTC	4 cut(s)	76, 233, 1076, 1220
BfaI	CTAG	1 cut(s)	1023
BfmI	CTRYAG	1 cut(s)	1509
BfoI	RGCGCY	1 cut(s)	173
BisI	GCNGC	5 cut(s)	57, 173, 631, 1827, 1892
BlsI	GCNGC	5 cut(s)	58, 174, 632, 1828, 1893
Bme18I	GGWCC	9 cut(s)	161, 541, 576, 582, 791, 910, 966, 1529, 1650
BmeT110I	CYCGRG	2 cut(s)	261, 913
BmgBI	CACGTC	1 cut(s)	984
BmsI	GCATC	2 cut(s)	550, 1163
BpiI	GAAGAC	3 cut(s)	51, 1344, 1663
BpuEI	CTTGAG	2 cut(s)	1363, 1865
BpuMI	CCSGG	9 cut(s)	39, 165, 455, 580, 745, 914, 915, 950, 1580
Bsa29I	ATCGAT	1 cut(s)	550
BsaAI	YACGTR	1 cut(s)	1012
BsaHI	GRCGYC	2 cut(s)	170, 1617
BsaI	GGTCTC	1 cut(s)	76
BsaJI	CCNNGG	9 cut(s)	38, 157, 164, 537, 913, 969, 1271, 1578, 1656
BsaWI	WCCGGW	1 cut(s)	196
BsaXI	ACNNNNNCTCC	4 cut(s)	1680, 1710, 1851, 1881
Bse118I	RCCGGY	1 cut(s)	1321
Bse1I	ACTGG	6 cut(s)	231, 445, 521, 631, 844, 1303
Bse3DI	GCAATG	1 cut(s)	1548
BseBI	CCWGG	1 cut(s)	349
BseCI	ATCGAT	1 cut(s)	550
BseDI	CCNNGG	9 cut(s)	38, 157, 164, 537, 913, 969, 1271, 1578, 1656
BseGI	GGATG	5 cut(s)	431, 740, 1036, 1714, 1912
BseMI	GCAATG	1 cut(s)	1548
BseMII	CTCAG	3 cut(s)	474, 1215, 1914
BseNI	ACTGG	6 cut(s)	231, 445, 521, 631, 844, 1303
BseRI	GAGGAG	4 cut(s)	103, 584, 1343, 1484
BseXI	GCAGC	3 cut(s)	642, 1838, 1903
Bsh1236I	CGCG	3 cut(s)	782, 1766, 1795
Bsh1285I	CGRYCG	3 cut(s)	550, 1608, 1752
BshFI	GGCC	6 cut(s)	220, 287, 409, 489, 606, 1321
BshNI	GGYRCC	2 cut(s)	169, 1090
BshVI	ATCGAT	1 cut(s)	550
BsiEI	CGRYCG	3 cut(s)	550, 1608, 1752
BsiHKAI	GWGCWC	2 cut(s)	888, 1523
BsiHKCI	CYCGRG	2 cut(s)	261, 913
BslFI	GGGAC	6 cut(s)	47, 527, 777, 896, 1075, 1628
BsmAI	GTCTC	4 cut(s)	76, 233, 1076, 1220
BsmBI	CGTCTC	1 cut(s)	233
BsmFI	GGGAC	6 cut(s)	47, 527, 777, 896, 1075, 1628
BsmI	GAATGC	1 cut(s)	362
BsnI	GGCC	6 cut(s)	220, 287, 409, 489, 606, 1321
Bso31I	GGTCTC	1 cut(s)	76
BsoBI	CYCGRG	2 cut(s)	261, 913
Bsp1286I	GDGCHC	2 cut(s)	888, 1523
Bsp1407I	TGTACA	2 cut(s)	621, 855
Bsp19I	CCATGG	1 cut(s)	537
BspANI	GGCC	6 cut(s)	220, 287, 409, 489, 606, 1321
BspCNI	CTCAG	3 cut(s)	473, 1216, 1913
BspDI	ATCGAT	1 cut(s)	550
BspFNI	CGCG	3 cut(s)	782, 1766, 1795
BspT107I	GGYRCC	2 cut(s)	169, 1090
BspTNI	GGTCTC	1 cut(s)	76
BsrBI	CCGCTC	1 cut(s)	532
BsrDI	GCAATG	1 cut(s)	1548
BsrFI	RCCGGY	1 cut(s)	1321
BsrGI	TGTACA	2 cut(s)	621, 855
BsrI	ACTGG	6 cut(s)	231, 445, 521, 631, 844, 1303
BssAI	RCCGGY	1 cut(s)	1321
BssECI	CCNNGG	9 cut(s)	38, 157, 164, 537, 913, 969, 1271, 1578, 1656
BssNI	GRCGYC	2 cut(s)	170, 1617
BssT1I	CCWWGG	4 cut(s)	157, 537, 969, 1656
Bst2UI	CCWGG	1 cut(s)	349
Bst4CI	ACNGT	6 cut(s)	184, 1279, 1355, 1393, 1425, 1783
BstACI	GRCGYC	2 cut(s)	170, 1617
BstAUI	TGTACA	2 cut(s)	621, 855
BstBAI	YACGTR	1 cut(s)	1012
BstC8I	GCNNGC	2 cut(s)	222, 534
BstDEI	CTNAG	3 cut(s)	460, 1224, 1900
BstDSI	CCRYGG	1 cut(s)	537
BstF5I	GGATG	5 cut(s)	431, 740, 1036, 1714, 1912
BstFNI	CGCG	3 cut(s)	782, 1766, 1795
BstH2I	RGCGCY	1 cut(s)	173
BstHHI	GCGC	3 cut(s)	172, 1637, 1768
BstMAI	GTCTC	4 cut(s)	76, 233, 1076, 1220
BstMCI	CGRYCG	3 cut(s)	550, 1608, 1752
BstMWI	GCNNNNNNNGC	2 cut(s)	671, 1318
BstNI	CCWGG	1 cut(s)	349
BstSFI	CTRYAG	1 cut(s)	1509
BstUI	CGCG	3 cut(s)	782, 1766, 1795
BstV1I	GCAGC	3 cut(s)	642, 1838, 1903
BstV2I	GAAGAC	3 cut(s)	51, 1344, 1663
BstX2I	RGATCY	3 cut(s)	685, 752, 1191
BstXI	CCANNNNNNTGG	3 cut(s)	976, 1426, 1735
BstYI	RGATCY	3 cut(s)	685, 752, 1191
Bsu15I	ATCGAT	1 cut(s)	550
BsuRI	GGCC	6 cut(s)	220, 287, 409, 489, 606, 1321
BsuTUI	ATCGAT	1 cut(s)	550
BtgI	CCRYGG	1 cut(s)	537
BtgZI	GCGATG	1 cut(s)	1776
BtrI	CACGTC	1 cut(s)	984
BtsCI	GGATG	5 cut(s)	431, 740, 1036, 1714, 1912
BtsIMutI	CAGTG	7 cut(s)	189, 261, 514, 624, 756, 864, 1221
Cac8I	GCNNGC	2 cut(s)	222, 534
CfoI	GCGC	3 cut(s)	172, 1637, 1768
Cfr10I	RCCGGY	1 cut(s)	1321
Cfr9I	CCCGGG	1 cut(s)	913
ClaI	ATCGAT	1 cut(s)	550
CviAII	CATG	8 cut(s)	340, 483, 491, 508, 538, 890, 905, 1086
DdeI	CTNAG	3 cut(s)	460, 1224, 1900
DinI	GGCGCC	1 cut(s)	171
DrdI	GACNNNNNNGTC	1 cut(s)	1436
DseDI	GACNNNNNNGTC	1 cut(s)	1436
EaeI	YGGCCR	3 cut(s)	285, 604, 1319
EciI	GGCGGA	5 cut(s)	43, 57, 574, 726, 1014
Ecl136II	GAGCTC	1 cut(s)	886
Eco130I	CCWWGG	4 cut(s)	157, 537, 969, 1656
Eco24I	GRGCYC	1 cut(s)	888
Eco31I	GGTCTC	1 cut(s)	76
Eco47I	GGWCC	9 cut(s)	161, 541, 576, 582, 791, 910, 966, 1529, 1650
Eco53kI	GAGCTC	1 cut(s)	886
Eco57I	CTGAAG	2 cut(s)	1037, 1686
Eco72I	CACGTG	1 cut(s)	1012
Eco88I	CYCGRG	2 cut(s)	261, 913
EcoICRI	GAGCTC	1 cut(s)	886
EcoO109I	RGGNCCY	2 cut(s)	910, 1650
EcoRII	CCWGG	1 cut(s)	347
EcoT14I	CCWWGG	4 cut(s)	157, 537, 969, 1656
EcoT22I	ATGCAT	1 cut(s)	1156
EcoT38I	GRGCYC	1 cut(s)	888
EgeI	GGCGCC	1 cut(s)	171
EheI	GGCGCC	1 cut(s)	171
ErhI	CCWWGG	4 cut(s)	157, 537, 969, 1656
Esp3I	CGTCTC	1 cut(s)	233
FaeI	CATG	8 cut(s)	343, 486, 494, 511, 541, 893, 908, 1089
FaqI	GGGAC	6 cut(s)	47, 527, 777, 896, 1075, 1628
FatI	CATG	8 cut(s)	339, 482, 490, 507, 537, 889, 904, 1085
FauI	CCCGC	2 cut(s)	537, 1788
FbaI	TGATCA	2 cut(s)	1171, 1741
FblI	GTMKAC	1 cut(s)	1440
Fnu4HI	GCNGC	5 cut(s)	57, 173, 631, 1827, 1892
FokI	GGATG	5 cut(s)	438, 727, 1043, 1721, 1899
FriOI	GRGCYC	1 cut(s)	888
Fsp4HI	GCNGC	5 cut(s)	57, 173, 631, 1827, 1892
FspBI	CTAG	1 cut(s)	1023
GlaI	GCGC	3 cut(s)	171, 1636, 1767
GluI	GCNGC	5 cut(s)	57, 173, 631, 1827, 1892
HaeII	RGCGCY	1 cut(s)	173
HaeIII	GGCC	6 cut(s)	220, 287, 409, 489, 606, 1321
HhaI	GCGC	3 cut(s)	172, 1637, 1768
Hin1I	GRCGYC	2 cut(s)	170, 1617
Hin1II	CATG	8 cut(s)	343, 486, 494, 511, 541, 893, 908, 1089
Hin6I	GCGC	3 cut(s)	170, 1635, 1766
HinP1I	GCGC	3 cut(s)	170, 1635, 1766
HincII	GTYRAC	2 cut(s)	1441, 1575
HindII	GTYRAC	2 cut(s)	1441, 1575
HindIII	AAGCTT	1 cut(s)	315
HinfI	GANTC	5 cut(s)	458, 511, 641, 1559, 1643
HpaI	GTTAAC	1 cut(s)	1575
HphI	GGTGA	4 cut(s)	194, 425, 1511, 1724
Hpy166II	GTNNAC	9 cut(s)	576, 624, 856, 858, 940, 991, 1441, 1575, 1801
Hpy188III	TCNNGA	4 cut(s)	794, 1621, 1664, 1882
Hpy8I	GTNNAC	9 cut(s)	576, 624, 856, 858, 940, 991, 1441, 1575, 1801
Hpy99I	CGWCG	4 cut(s)	988, 1115, 1442, 1643
HpyAV	CCTTC	7 cut(s)	269, 710, 1012, 1137, 1182, 1268, 1915
HpyCH4III	ACNGT	6 cut(s)	184, 1279, 1355, 1393, 1425, 1783
HpyCH4IV	ACGT	5 cut(s)	983, 1011, 1113, 1246, 1617
HpyCH4V	TGCA	6 cut(s)	362, 649, 904, 1154, 1553, 1853
HpyF10VI	GCNNNNNNNGC	2 cut(s)	671, 1318
HpyF3I	CTNAG	3 cut(s)	460, 1224, 1900
HpySE526I	ACGT	5 cut(s)	983, 1011, 1113, 1246, 1617
Hsp92I	GRCGYC	2 cut(s)	170, 1617
Hsp92II	CATG	8 cut(s)	343, 486, 494, 511, 541, 893, 908, 1089
HspAI	GCGC	3 cut(s)	170, 1635, 1766
KasI	GGCGCC	1 cut(s)	169
KpnI	GGTACC	1 cut(s)	1094
Ksp22I	TGATCA	2 cut(s)	1171, 1741
KspAI	GTTAAC	1 cut(s)	1575
LmnI	GCTCC	4 cut(s)	891, 1518, 1666, 1823
Lsp1109I	GCAGC	3 cut(s)	642, 1838, 1903
LweI	GCATC	2 cut(s)	550, 1163
MaeI	CTAG	1 cut(s)	1023
MaeII	ACGT	5 cut(s)	983, 1011, 1113, 1246, 1617
MaeIII	GTNAC	4 cut(s)	139, 767, 1594, 1618
MbiI	CCGCTC	1 cut(s)	532
MboII	GAAGA	4 cut(s)	56, 1252, 1349, 1663
MfeI	CAATTG	1 cut(s)	1056
MflI	RGATCY	3 cut(s)	685, 752, 1191
MhlI	GDGCHC	2 cut(s)	888, 1523
Mly113I	GGCGCC	1 cut(s)	170
MlyI	GAGTC	1 cut(s)	452
MmeI	TCCRAC	3 cut(s)	1184, 1407, 1686
Mph1103I	ATGCAT	1 cut(s)	1156
MseI	TTAA	7 cut(s)	90, 731, 1005, 1209, 1349, 1574, 1936
MspA1I	CMGCKG	1 cut(s)	464
MunI	CAATTG	1 cut(s)	1056
Mva1269I	GAATGC	1 cut(s)	362
MvaI	CCWGG	1 cut(s)	349
MvnI	CGCG	3 cut(s)	782, 1766, 1795
MwoI	GCNNNNNNNGC	2 cut(s)	671, 1318
NarI	GGCGCC	1 cut(s)	170
NciI	CCSGG	9 cut(s)	39, 165, 455, 580, 745, 914, 915, 950, 1580
NcoI	CCATGG	1 cut(s)	537
NlaIII	CATG	8 cut(s)	343, 486, 494, 511, 541, 893, 908, 1089
NmuCI	GTSAC	3 cut(s)	139, 767, 1618
NsiI	ATGCAT	1 cut(s)	1156
PaeR7I	CTCGAG	1 cut(s)	261
PcsI	WCGNNNNNNNCGW	1 cut(s)	290
PctI	GAATGC	1 cut(s)	362
PfeI	GAWTC	4 cut(s)	511, 641, 1559, 1643
PflFI	GACNNNGTC	2 cut(s)	244, 580
PfoI	TCCNGGA	1 cut(s)	743
PkrI	GCNGC	5 cut(s)	58, 174, 632, 1828, 1893
Ple19I	CGATCG	2 cut(s)	550, 1752
PleI	GAGTC	1 cut(s)	452
PluTI	GGCGCC	1 cut(s)	173
PmaCI	CACGTG	1 cut(s)	1012
PmlI	CACGTG	1 cut(s)	1012
PpsI	GAGTC	1 cut(s)	452
Ppu21I	YACGTR	1 cut(s)	1012
PpuMI	RGGWCCY	2 cut(s)	910, 1650
PshBI	ATTAAT	1 cut(s)	1209
PsiI	TTATAA	1 cut(s)	875
Psp124BI	GAGCTC	1 cut(s)	888
Psp1406I	AACGTT	1 cut(s)	1246
Psp5II	RGGWCCY	2 cut(s)	910, 1650
Psp6I	CCWGG	1 cut(s)	347
PspCI	CACGTG	1 cut(s)	1012
PspGI	CCWGG	1 cut(s)	347
PspPPI	RGGWCCY	2 cut(s)	910, 1650
PspXI	VCTCGAGB	1 cut(s)	261
PsrI	GAACNNNNNNTAC	2 cut(s)	422, 454
PsuI	RGATCY	3 cut(s)	685, 752, 1191
PsyI	GACNNNGTC	2 cut(s)	244, 580
PvuI	CGATCG	2 cut(s)	550, 1752
PvuII	CAGCTG	1 cut(s)	464
SacI	GAGCTC	1 cut(s)	888
SalI	GTCGAC	1 cut(s)	1439
SaqAI	TTAA	7 cut(s)	90, 731, 1005, 1209, 1349, 1574, 1936
SatI	GCNGC	5 cut(s)	57, 173, 631, 1827, 1892
SchI	GAGTC	1 cut(s)	452
SduI	GDGCHC	2 cut(s)	888, 1523
SfaNI	GCATC	2 cut(s)	550, 1163
SfcI	CTRYAG	1 cut(s)	1509
SfoI	GGCGCC	1 cut(s)	171
Sfr274I	CTCGAG	1 cut(s)	261
SinI	GGWCC	9 cut(s)	161, 541, 576, 582, 791, 910, 966, 1529, 1650
SlaI	CTCGAG	1 cut(s)	261
SmaI	CCCGGG	1 cut(s)	915
SmlI	CTYRAG	3 cut(s)	261, 1342, 1880
SmoI	CTYRAG	3 cut(s)	261, 1342, 1880
SspDI	GGCGCC	1 cut(s)	169
SspMI	CTAG	1 cut(s)	1023
SstI	GAGCTC	1 cut(s)	888
StyI	CCWWGG	4 cut(s)	157, 537, 969, 1656
TaaI	ACNGT	6 cut(s)	184, 1279, 1355, 1393, 1425, 1783
TaiI	ACGT	5 cut(s)	986, 1014, 1116, 1249, 1620
TaqII	GACCGA	1 cut(s)	1622
TatI	WGTACW	4 cut(s)	447, 621, 652, 855
TauI	GCSGC	2 cut(s)	59, 175
TfiI	GAWTC	4 cut(s)	511, 641, 1559, 1643
Tru1I	TTAA	7 cut(s)	90, 731, 1005, 1209, 1349, 1574, 1936
Tru9I	TTAA	7 cut(s)	90, 731, 1005, 1209, 1349, 1574, 1936
TscAI	CASTG	7 cut(s)	189, 261, 521, 631, 763, 871, 1228
TseFI	GTSAC	3 cut(s)	139, 767, 1618
TseI	GCWGC	3 cut(s)	630, 1826, 1891
Tsp45I	GTSAC	3 cut(s)	139, 767, 1618
TspDTI	ATGAA	6 cut(s)	309, 1227, 1572, 1779, 1897, 1901
TspGWI	ACGGA	5 cut(s)	106, 518, 582, 634, 755
TspMI	CCCGGG	1 cut(s)	913
TspRI	CASTG	7 cut(s)	189, 261, 521, 631, 763, 871, 1228
Tth111I	GACNNNGTC	2 cut(s)	244, 580
VpaK11BI	GGWCC	9 cut(s)	161, 541, 576, 582, 791, 910, 966, 1529, 1650
VspI	ATTAAT	1 cut(s)	1209
XapI	RAATTY	3 cut(s)	827, 843, 1361
XcmI	CCANNNNNNNNNTGG	1 cut(s)	384
XhoI	CTCGAG	1 cut(s)	261
XmaI	CCCGGG	1 cut(s)	913
XmiI	GTMKAC	1 cut(s)	1440
XspI	CTAG	1 cut(s)	1023
ZraI	GACGTC	1 cut(s)	1618
Zsp2I	ATGCAT	1 cut(s)	1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.