Rh1CG372100

Belongs to the glycosyl hydrolase 32 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013719
Physical Location & Seq
Forward (+)
64179985 .. 64182236
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG372100.1

Sequence Viewer

Length: 1467 bp
ATGAAACTTAATCAATGTTTTTTTTATTTTGTAGGTCCATTGTTTTACAAGGGTTGGTATCACCTGTTCTACCAGTACAATCCGGACTCCGCCATATGGGGCAACATCACGTGGGGCCACGCCGTATCGACGGACCTGATCCACTGGCTATACCTCCCCATCGCAATGGTCGCTGATCAATGGTACGATGCGAATGGCGTGTGGAGCGGGTCCGCCACGCTACTCCCGGACGGTCAGATCGTAATGTTGTACACGGGGGACACCGTCGATGCTGTTCAAGTGGTGTGCCTTGCGCACCCGGCCAACCTGTCTGATCCCTTACTCCTAGATTGGGTCAAGTACTCGGGTAACCCGGTGTTGACTCCTCCGCCCGGCATTTTAACCACCGATTTCCGTGACCCCACCACAGCGTGGACCGGACCGGACGGCAAGTGGCGCATTACTATTGGCTCAAAGGTCAATACAACCGGTATTTCCTTTGTGTACCACACAGAGGATTTCAAAACCTACAATATGTCGAAGGGGGTCTTGCATGCTGTTCCGGGTACGGGTATGTGGGAGTGCATTGACTTTTACCCGGTTGCCATAAACGGGTCGAAGGGTGTGGAAACGTCCGTGAATAACCCGAGCGTCAAGCATGTCCTGAAGGCGAGCTTGGACAACACAAAAGTGGATCACTATGCACTTGGGACTTATTTTGAGGAGAATGAAACATGGGTACCCGATAACCCGGCTCTTGATGTGGGTATTGGGTTGAGGTATGACTATGGAAGATACTATGCATCAAAGACATTTTATGACCAGAACAAGGAGAGAAGGATCCTGTGGGGTTGGATCAATGAGACTGATACCGAATCCGATGACTTGGCAAAAGGTTGGGCATCTGTTCAGACTATTCCGAGGACTGTGTTGTTTGACAATAAAACCGGCACCAATTTGATCCAATGGCCAGTGGAAGAGATAGAGGAGTTGAGATTGAACAATACCGACTTCAGTGATGTTCTAGTTGAAGCAGGAACAGTTGTTGAGCTAGATATTGGAACAGCTACACAGTTGGACATTTTAGTGGAGTTTGAATTAGAACCTTTGGAATCATCAGAGACTGTGAATAGCAGTGTGGGTTGTGGTGGTGGTGCTGTAGATAGAGGCACGTTTGGACCATTTGGTATTCTGGTCATTGCAGATGAGACTCTTACTGAGCTAACCCCTATCTATTTCAACCTCGCCAATTCAACTGAAGGGGACGTCATAACTTATTTCTGCGCAGACGAAAGAAGGTCATCTAAGGCTCCTGATGTCTTCAAACAAGTTTATGGAAGTGAAGTTCCTGTGTTGGATGGTGAAAAACACTTCGCAAGAGTGTTGGTGCGTATCTTGATCTCCCTAACAAGTTTATATGTTCTTTTTAGTATATACGAGTGCCAAATAGAAATAGTCAAAACATTTCAGGTTGTCGACTTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

488

Amino Acids

54.61

Weight (kDa)

4.51

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_32N PF00251 12 - 317 4.4e-91 Glycosyl hydrolases family 32 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1248
Acc16I TGCGCA 2 cut(s) 294, 1264
Acc65I GGTACC 1 cut(s) 718
AccB1I GGYRCC 2 cut(s) 718, 929
AccB7I CCANNNNNTGG 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 1455
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 4 cut(s) 90, 207, 213, 368
AclWI GGATC 7 cut(s) 133, 308, 681, 814, 827, 842, 934
AcoI YGGCCR 2 cut(s) 300, 947
AcuI CTGAAG 3 cut(s) 665, 976, 1257
AcvI CACGTG 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 1245
AdeI CACNNNGTG 1 cut(s) 411
AfaI GTAC 7 cut(s) 77, 185, 251, 341, 485, 547, 720
AfiI CCNNNNNNNGG 8 cut(s) 96, 331, 371, 411, 493, 548, 591, 808
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 8 cut(s) 278, 502, 979, 1010, 1076, 1219, 1233, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 638, 670
AleI CACNNNNGTG 1 cut(s) 668
AloI GAACNNNNNNTCC 2 cut(s) 1308, 1340
AluBI AGCT 4 cut(s) 654, 1030, 1046, 1201
AluI AGCT 4 cut(s) 654, 1030, 1046, 1201
Alw26I GTCTC 3 cut(s) 836, 1094, 1181
AlwI GGATC 7 cut(s) 133, 308, 681, 814, 827, 842, 934
AlwNI CAGNNNCTG 1 cut(s) 1103
Ama87I CYCGRG 2 cut(s) 343, 625
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 3 cut(s) 115, 300, 947
AsiGI ACCGGT 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 718
AspLEI GCGC 3 cut(s) 295, 438, 1265
AspS9I GGNCC 7 cut(s) 35, 115, 133, 210, 414, 419, 1157
AsuC2I CCSGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
AsuHPI GGTGA 2 cut(s) 53, 1352
AvaI CYCGRG 2 cut(s) 343, 625
AvaII GGWCC 6 cut(s) 35, 133, 210, 414, 419, 1157
BaeI ACNNNNGTAYC 2 cut(s) 702, 735
BalI TGGCCA 1 cut(s) 949
BamHI GGATCC 1 cut(s) 819
BanI GGYRCC 2 cut(s) 718, 929
BbrPI CACGTG 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 1291
BccI CCATC 2 cut(s) 167, 1331
BceAI ACGGC 2 cut(s) 107, 442
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
BcoDI GTCTC 3 cut(s) 836, 1094, 1181
BfaI CTAG 3 cut(s) 326, 1004, 1031
BfmI CTRYAG 1 cut(s) 1137
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
Bme18I GGWCC 6 cut(s) 35, 133, 210, 414, 419, 1157
BmeT110I CYCGRG 2 cut(s) 343, 625
BmgT120I GGNCC 7 cut(s) 35, 115, 133, 210, 414, 419, 1157
BmiI GGNNCC 6 cut(s) 116, 211, 720, 821, 931, 1290
BmrFI CCNGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
BmsI GCATC 4 cut(s) 178, 259, 791, 890
BpiI GAAGAC 1 cut(s) 1291
BpuMI CCSGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
BsaAI YACGTR 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 1245
BsaJI CCNNGG 1 cut(s) 899
BsaWI WCCGGW 4 cut(s) 82, 416, 421, 467
Bsc4I CCNNNNNNNGG 8 cut(s) 96, 331, 371, 411, 493, 548, 591, 808
Bse118I RCCGGY 2 cut(s) 467, 926
Bse1I ACTGG 3 cut(s) 73, 149, 950
Bse3DI GCAATG 2 cut(s) 171, 1176
BseAI TCCGGA 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 899
BseGI GGATG 1 cut(s) 1342
BseLI CCNNNNNNNGG 8 cut(s) 96, 331, 371, 411, 493, 548, 591, 808
BseMI GCAATG 2 cut(s) 171, 1176
BseMII CTCAG 1 cut(s) 1188
BseNI ACTGG 3 cut(s) 73, 149, 950
BseRI GAGGAG 3 cut(s) 354, 716, 980
BshFI GGCC 3 cut(s) 117, 302, 949
BshNI GGYRCC 2 cut(s) 718, 929
BshTI ACCGGT 1 cut(s) 467
BsiHKCI CYCGRG 2 cut(s) 343, 625
BslFI GGGAC 3 cut(s) 272, 703, 1256
BslI CCNNNNNNNGG 8 cut(s) 96, 331, 371, 411, 493, 548, 591, 808
BsmAI GTCTC 3 cut(s) 836, 1094, 1181
BsmFI GGGAC 3 cut(s) 272, 703, 1256
BsnI GGCC 3 cut(s) 117, 302, 949
BsoBI CYCGRG 2 cut(s) 343, 625
Bsp13I TCCGGA 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
BspACI CCGC 4 cut(s) 90, 207, 213, 368
BspANI GGCC 3 cut(s) 117, 302, 949
BspCNI CTCAG 1 cut(s) 1189
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 6 cut(s) 116, 211, 720, 821, 931, 1290
BspPI GGATC 7 cut(s) 133, 308, 681, 814, 827, 842, 934
BspT107I GGYRCC 2 cut(s) 718, 929
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 171, 1176
BsrFI RCCGGY 2 cut(s) 467, 926
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 3 cut(s) 73, 149, 950
BssAI RCCGGY 2 cut(s) 467, 926
BssECI CCNNGG 1 cut(s) 899
BssMI GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
BssNI GRCGYC 1 cut(s) 1245
Bst4CI ACNGT 6 cut(s) 233, 265, 907, 1021, 1053, 1105
Bst6I CTCTTC 1 cut(s) 951
BstACI GRCGYC 1 cut(s) 1245
BstAUI TGTACA 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 534, 652
BstDEI CTNAG 2 cut(s) 1197, 1284
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 1342
BstHHI GCGC 3 cut(s) 295, 438, 1265
BstKTI GATC 9 cut(s) 141, 178, 240, 316, 676, 822, 837, 942, 1380
BstMAI GTCTC 3 cut(s) 836, 1094, 1181
BstMBI GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
BstMWI GCNNNNNNNGC 4 cut(s) 170, 204, 299, 435
BstNSI RCATGY 2 cut(s) 536, 641
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 7 cut(s) 225, 297, 351, 370, 541, 576, 729
BstSFI CTRYAG 1 cut(s) 1137
BstV2I GAAGAC 1 cut(s) 1291
BstX2I RGATCY 1 cut(s) 819
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 1 cut(s) 819
BsuRI GGCC 3 cut(s) 117, 302, 949
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 1342
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 4 cut(s) 142, 957, 1000, 1120
Cac8I GCNNGC 2 cut(s) 534, 652
CaiI CAGNNNCTG 1 cut(s) 1103
CfoI GCGC 3 cut(s) 295, 438, 1265
Cfr10I RCCGGY 2 cut(s) 467, 926
Cfr13I GGNCC 7 cut(s) 35, 115, 133, 210, 414, 419, 1157
CpoI CGGWCCG 1 cut(s) 419
CseI GACGC 1 cut(s) 619
Csp6I GTAC 7 cut(s) 76, 184, 250, 340, 484, 546, 719
CspAI ACCGGT 1 cut(s) 467
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 3 cut(s) 533, 638, 714
CviQI GTAC 7 cut(s) 76, 184, 250, 340, 484, 546, 719
DdeI CTNAG 2 cut(s) 1197, 1284
DpnI GATC 9 cut(s) 140, 177, 239, 315, 675, 821, 836, 941, 1379
DpnII GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
DraIII CACNNNGTG 1 cut(s) 411
EaeI YGGCCR 2 cut(s) 300, 947
Eam1104I CTCTTC 1 cut(s) 951
EarI CTCTTC 1 cut(s) 951
EciI GGCGGA 3 cut(s) 79, 202, 357
Eco47I GGWCC 6 cut(s) 35, 133, 210, 414, 419, 1157
Eco57I CTGAAG 3 cut(s) 665, 976, 1257
Eco72I CACGTG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 343, 625
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoT22I ATGCAT 1 cut(s) 784
FaeI CATG 3 cut(s) 536, 641, 717
FalI AAGNNNNNCTT 4 cut(s) 512, 544, 638, 670
FaqI GGGAC 3 cut(s) 272, 703, 1256
FatI CATG 3 cut(s) 532, 637, 713
FauI CCCGC 1 cut(s) 200
FauNDI CATATG 1 cut(s) 95
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 1455
FokI GGATG 1 cut(s) 1349
FspBI CTAG 3 cut(s) 326, 1004, 1031
FspI TGCGCA 2 cut(s) 294, 1264
GlaI GCGC 3 cut(s) 294, 437, 1264
HaeIII GGCC 3 cut(s) 117, 302, 949
HgaI GACGC 1 cut(s) 619
HhaI GCGC 3 cut(s) 295, 438, 1265
Hin1I GRCGYC 1 cut(s) 1245
Hin1II CATG 3 cut(s) 536, 641, 717
Hin6I GCGC 3 cut(s) 293, 436, 1263
HinP1I GCGC 3 cut(s) 293, 436, 1263
HincII GTYRAC 2 cut(s) 360, 1456
HindII GTYRAC 2 cut(s) 360, 1456
HinfI GANTC 5 cut(s) 86, 361, 854, 1091, 1189
HphI GGTGA 2 cut(s) 53, 1352
Hpy166II GTNNAC 5 cut(s) 252, 360, 414, 484, 1456
Hpy188I TCNGA 6 cut(s) 237, 313, 859, 891, 900, 1099
Hpy188III TCNNGA 5 cut(s) 83, 643, 737, 1292, 1375
Hpy8I GTNNAC 5 cut(s) 252, 360, 414, 484, 1456
Hpy99I CGWCG 2 cut(s) 133, 269
HpyAV CCTTC 6 cut(s) 514, 592, 640, 810, 1232, 1269
HpyCH4III ACNGT 6 cut(s) 233, 265, 907, 1021, 1053, 1105
HpyCH4IV ACGT 4 cut(s) 110, 611, 1151, 1245
HpyCH4V TGCA 5 cut(s) 532, 564, 683, 782, 1181
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 204, 299, 435
HpyF3I CTNAG 2 cut(s) 1197, 1284
HpySE526I ACGT 4 cut(s) 110, 611, 1151, 1245
Hsp92I GRCGYC 1 cut(s) 1245
Hsp92II CATG 3 cut(s) 536, 641, 717
HspAI GCGC 3 cut(s) 293, 436, 1263
Kpn2I TCCGGA 1 cut(s) 82
KpnI GGTACC 1 cut(s) 722
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
LmnI GCTCC 2 cut(s) 204, 1294
LweI GCATC 4 cut(s) 178, 259, 791, 890
MaeI CTAG 3 cut(s) 326, 1004, 1031
MaeII ACGT 4 cut(s) 110, 611, 1151, 1245
MaeIII GTNAC 2 cut(s) 347, 395
MalI GATC 9 cut(s) 140, 177, 239, 315, 675, 821, 836, 941, 1379
MbiI CCGCTC 1 cut(s) 207
MboI GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
MboII GAAGA 3 cut(s) 783, 968, 1291
MflI RGATCY 1 cut(s) 819
MlsI TGGCCA 1 cut(s) 949
MluCI AATT 3 cut(s) 934, 1076, 1228
MluNI TGGCCA 1 cut(s) 949
MlyI GAGTC 3 cut(s) 80, 355, 1183
MmeI TCCRAC 3 cut(s) 812, 1035, 1314
MnlI CCTC 9 cut(s) 164, 375, 487, 694, 750, 894, 958, 1139, 1232
Mox20I TGGCCA 1 cut(s) 949
Mph1103I ATGCAT 1 cut(s) 784
MroI TCCGGA 1 cut(s) 82
MscI TGGCCA 1 cut(s) 949
MseI TTAA 3 cut(s) 9, 380, 1460
MslI CAYNNNNRTG 3 cut(s) 164, 668, 1064
Msp20I TGGCCA 1 cut(s) 949
MspR9I CCNGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
MwoI GCNNNNNNNGC 4 cut(s) 170, 204, 299, 435
NciI CCSGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
NdeI CATATG 1 cut(s) 95
NdeII GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
NlaIII CATG 3 cut(s) 536, 641, 717
NlaIV GGNNCC 6 cut(s) 116, 211, 720, 821, 931, 1290
NmuCI GTSAC 1 cut(s) 395
NsbI TGCGCA 2 cut(s) 294, 1264
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 2 cut(s) 536, 641
OliI CACNNNNGTG 1 cut(s) 668
PaeI GCATGC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 2 cut(s) 854, 1091
PflFI GACNNNGTC 1 cut(s) 263
PflMI CCANNNNNTGG 1 cut(s) 411
PfoI TCCNGGA 1 cut(s) 225
PinAI ACCGGT 1 cut(s) 467
PleI GAGTC 3 cut(s) 80, 355, 1183
PmaCI CACGTG 1 cut(s) 111
PmlI CACGTG 1 cut(s) 111
PpsI GAGTC 3 cut(s) 80, 355, 1183
Ppu21I YACGTR 1 cut(s) 111
PspCI CACGTG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 6 cut(s) 116, 211, 720, 821, 931, 1290
PspPI GGNCC 7 cut(s) 35, 115, 133, 210, 414, 419, 1157
PsrI GAACNNNNNNTAC 2 cut(s) 50, 82
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 1 cut(s) 819
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 7 cut(s) 77, 185, 251, 341, 485, 547, 720
RsaNI GTAC 7 cut(s) 76, 184, 250, 340, 484, 546, 719
RseI CAYNNNNRTG 3 cut(s) 164, 668, 1064
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SalI GTCGAC 1 cut(s) 1454
SaqAI TTAA 3 cut(s) 9, 380, 1460
Sau3AI GATC 9 cut(s) 138, 175, 237, 313, 673, 819, 834, 939, 1377
Sau96I GGNCC 7 cut(s) 35, 115, 133, 210, 414, 419, 1157
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 3 cut(s) 80, 355, 1183
ScrFI CCNGG 7 cut(s) 227, 299, 353, 372, 543, 578, 731
SfaNI GCATC 4 cut(s) 178, 259, 791, 890
SfcI CTRYAG 1 cut(s) 1137
SinI GGWCC 6 cut(s) 35, 133, 210, 414, 419, 1157
SmiMI CAYNNNNRTG 3 cut(s) 164, 668, 1064
SphI GCATGC 1 cut(s) 536
Sse9I AATT 3 cut(s) 934, 1076, 1228
SsiI CCGC 4 cut(s) 90, 207, 213, 368
SspMI CTAG 3 cut(s) 326, 1004, 1031
StyD4I CCNGG 7 cut(s) 225, 297, 351, 370, 541, 576, 729
TaaI ACNGT 6 cut(s) 233, 265, 907, 1021, 1053, 1105
TaiI ACGT 4 cut(s) 113, 614, 1154, 1248
TaqI TCGA 5 cut(s) 128, 267, 518, 596, 1455
TasI AATT 3 cut(s) 934, 1076, 1228
TatI WGTACW 3 cut(s) 75, 249, 339
TfiI GAWTC 2 cut(s) 854, 1091
Tru1I TTAA 3 cut(s) 9, 380, 1460
Tru9I TTAA 3 cut(s) 9, 380, 1460
TscAI CASTG 4 cut(s) 149, 957, 1000, 1120
TseFI GTSAC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 17, 723
TspGWI ACGGA 3 cut(s) 146, 383, 604
TspRI CASTG 4 cut(s) 149, 957, 1000, 1120
Tth111I GACNNNGTC 1 cut(s) 263
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 6 cut(s) 35, 133, 210, 414, 419, 1157
XceI RCATGY 2 cut(s) 536, 641
XmiI GTMKAC 1 cut(s) 1455
XspI CTAG 3 cut(s) 326, 1004, 1031
ZraI GACGTC 1 cut(s) 1246
ZrmI AGTACT 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.