Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0013719

Physical Location & Seq

Genomic Coordinates Reverse (-)

5395853 .. 5399289

Gene Structure 3437 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026733

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGACACTAGTACTTCTGCCTACGCTCCCTTGCCGGGAGAAGACCCTTTATTTTCCGGTCCTCCCCCGGCAAGTCTCCGACGATCCTGGAAGGGCTTCGCCGTGATCTTCGCCTCCGTCTTGTTTCTCTTGTCATTGGTCGGACTAATCATCCATCAAGGCCCGCAACAACCGCCGAATGTAATGCCGGATAAACAAGATGAACATCATCATCCTCAGTCAACTACTCCAGCTTCAGAAACTACGTCGTCCTGGGAGCCGAGAGGGAAGGCACTAGGAGTCTCGGCCAAGTCGAACCCGCCGGTCTCCGACGAGCTCTCCTATAACTGGACCAATGCTATGTTCTCTTGGCAGAGAACAGCTTTTCACTTTCAACCAGAACGGAATTGGATGAACGGTCCATTGTTTTACAAGGGTTGGTATCACCTGTTCTACCAGTACAATCCGGACTCGGCCATATGGGGCAACATCACGTGGGGCCACGCCGTATCGACGGACCTGATCCACTGGCTATACCTCCCCCTCGCAATGGTCGCCGATCAATGGTACGATGCGAATGGCGTGTGGAGCGGGTCCGCCACGCTACTCCCCGACGGTCAGATCGTAATGTTGTACACGGGGGACACCGTCGATGCGGTTCAAGTGGTGTGCCTTGCGTACCCGGCCAACCTATCTGATCCCCTGCTCCTAGATTGGGTCAAGTACTCGGGTAACCCGGTGTTGACTCCTCCGCCCGGCATTTTATCCACGGATTTCCGTGACCCCACCACAGCGTGGACCGGACCTGACGGCAAGTGGCGCATTACTATTGGCTCAAAGGTCAATACAACCGGTATTTCCTTTGTGTACCACACAGAGGATTTCAAAACCTACAATATGTCGAAGGGGGTCTTGCATGCGGTTCCGGGTACGGGTATGTGGGAGTGCATTGACTTTTACCCGGTTGCTATAAACGGGTCGAAGGGTGTGGAAACGTCCGTGAATAACCCGAGTGTCAAGCATGTCCTGAAGGCTAGCTTGGACGACACAAAAGTGGATTACTATGCACTTGGGACTTATTTTGAGGAGAATGAAACATGGGTACCCGATAACCCGGCTCTTGATGTGGGTATTGGGTTGAAGTATGACTATGGAAGATACTATGCATCAAAGACATTTTATGACCAGAACAAGGAGAGAAGGATCCTGTGGGGTTGGATCAATGAGACCGATACCGAATCCGATGACTTGGCAAAAGGTTGGGCATCTGTTCAGACTATTCCGAGGAGTGTGTTGTTTGACAATAAAACCGGCACCAATTTGATCCAATGGCCGGTGGAAGAGATCGAGGAGTTGAGATTGAACAGTACCGACTTCAGTGATGTTCTAGTTGAAGCAGGAACAGTTGTTGAACTAGATATTGGAACAGCTACGCAGTTGGACATTTTAGTGGAGTTTGAATTAGAACCGTTGGAATCATCAGAGACTGTGAATAGCAGTGTAGGTTGTGGCGGTGGTGCTGTAGATAGAGGCACATTTGGACCATTTGGTATTCTGGTCATTGCAGATGAGACTCTTACTGAGCTAACCCCTATCTATTTCAACCTCGCCAATTCAACTGAAGGGGACGTCACAACTTATTTCTGCGCAGACGAAAGAAGGTCATCTAAGGCTCCTGATGTCTTCAAACAAGTTTATGGAAGTGAAGTTCCTGTGTTGGATGGTGAAAAACACTTCGCTAGAGTGTTGGTTGATCATTCAATTGTAGAGAGCTTTGCGCAAGGAGGTAGAACGGTGATTACATCACGAGTTTATCCAACAGAAGCAATCTACGGAGCAGCAAGGCTATTTTTGTTCAACAATGCAACAGGTCTAAATGTTAAGGCCACCCTCAAGATTTGGCAGCTAAACTCAGCTTTCATTCACCCTTTCCCATTAGACCAGATTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

DUF3357 Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

644

Amino Acids

71.39

Weight (kDa)

4.71

Isoelectric Point (pI)

33.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INV_N	PF11837	8 - 114	2.7e-15	Beta-fructofuranosidase, N-terminal domain
Glyco_hydro_32N	PF00251	123 - 438	1.2e-94	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	441 - 628	6e-20	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013719)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g27630 FvH4_7g27630 FvH4_7g27630
malus_domestica	MD07G1254500.v1.1 MD11G1195800.v1.1
prunus_persica	Prupe.2G277900_v2.0.a1 Prupe.2G277900_v2.0.a1 Prupe.2G277900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0374511
rosa_laevigata	RLG00000026733
rosa_multiflora	Rmu_ssc0000007.1_g000003
rosa_roxburghii	Rroxscaffold_4G00283440
rosa_rugosa	Rorug01G0383900
rosa_samantha	Rh1AG394400 Rh1BG359000 Rh1CG372100 Rh1DG389600
rosa_wichuraiana	Rw1G034170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1611
Acc16I	TGCGCA	2 cut(s)	1627, 1758
Acc65I	GGTACC	1 cut(s)	1081
AccB1I	GGYRCC	2 cut(s)	1081, 1292
AccB7I	CCANNNNNTGG	1 cut(s)	774
AccBSI	CCGCTC	1 cut(s)	570
AccIII	TCCGGA	1 cut(s)	445
AciI	CCGC	9 cut(s)	164, 173, 299, 570, 576, 635, 731, 899, 1491
AclWI	GGATC	7 cut(s)	78, 496, 671, 1177, 1190, 1205, 1297
AcoI	YGGCCR	4 cut(s)	285, 453, 663, 1310
AcuI	CTGAAG	4 cut(s)	219, 1028, 1339, 1620
AcvI	CACGTG	1 cut(s)	474
AcyI	GRCGYC	1 cut(s)	1608
AdeI	CACNNNGTG	1 cut(s)	774
AgeI	ACCGGT	1 cut(s)	830
AhlI	ACTAGT	1 cut(s)	8
AjnI	CCWGG	2 cut(s)	86, 251
AjuI	GAANNNNNNNTTGG	6 cut(s)	326, 358, 1001, 1033, 1383, 1415
AleI	CACNNNNGTG	1 cut(s)	1031
AloI	GAACNNNNNNTCC	2 cut(s)	1671, 1703
AluBI	AGCT	9 cut(s)	233, 316, 362, 1017, 1409, 1564, 1752, 1885, 1895
AluI	AGCT	9 cut(s)	233, 316, 362, 1017, 1409, 1564, 1752, 1885, 1895
Alw21I	GWGCWC	1 cut(s)	318
Alw26I	GTCTC	6 cut(s)	80, 286, 310, 1199, 1457, 1544
AlwI	GGATC	7 cut(s)	78, 496, 671, 1177, 1190, 1205, 1297
AlwNI	CAGNNNCTG	1 cut(s)	1466
Ama87I	CYCGRG	2 cut(s)	706, 988
Aor13HI	TCCGGA	1 cut(s)	445
AoxI	GGCC	7 cut(s)	160, 285, 453, 478, 663, 1310, 1863
ApeKI	GCWGC	2 cut(s)	1817, 1882
AsiGI	ACCGGT	1 cut(s)	830
Asp700I	GAANNNNTTC	1 cut(s)	95
Asp718I	GGTACC	1 cut(s)	1081
AspLEI	GCGC	3 cut(s)	801, 1628, 1759
AsuC2I	CCSGG	8 cut(s)	36, 68, 662, 716, 735, 906, 941, 1094
AsuHPI	GGTGA	4 cut(s)	416, 1715, 1786, 1895
AsuNHI	GCTAGC	1 cut(s)	1013
AvaI	CYCGRG	2 cut(s)	706, 988
AvaII	GGWCC	8 cut(s)	59, 330, 398, 496, 573, 777, 782, 1520
BaeI	ACNNNNGTAYC	2 cut(s)	1065, 1098
BamHI	GGATCC	1 cut(s)	1182
BanI	GGYRCC	2 cut(s)	1081, 1292
BanII	GRGCYC	1 cut(s)	318
BauI	CACGAG	1 cut(s)	1785
BbrPI	CACGTG	1 cut(s)	474
BbsI	GAAGAC	2 cut(s)	48, 1654
Bbv12I	GWGCWC	1 cut(s)	318
BbvI	GCAGC	2 cut(s)	1829, 1894
BccI	CCATC	2 cut(s)	162, 1694
BceAI	ACGGC	3 cut(s)	86, 470, 805
BcgI	CGANNNNNNTGC	2 cut(s)	166, 200
BciT130I	CCWGG	2 cut(s)	88, 253
BclI	TGATCA	1 cut(s)	1732
BcnI	CCSGG	8 cut(s)	36, 68, 662, 716, 735, 906, 941, 1094
BcoDI	GTCTC	6 cut(s)	80, 286, 310, 1199, 1457, 1544
BcuI	ACTAGT	1 cut(s)	8
BfaI	CTAG	7 cut(s)	9, 275, 689, 1014, 1367, 1394, 1719
BfmI	CTRYAG	1 cut(s)	1500
BisI	GCNGC	2 cut(s)	1818, 1883
BlsI	GCNGC	2 cut(s)	1819, 1884
BmcAI	AGTACT	2 cut(s)	13, 704
Bme18I	GGWCC	8 cut(s)	59, 330, 398, 496, 573, 777, 782, 1520
BmeT110I	CYCGRG	2 cut(s)	706, 988
BmiI	GGNNCC	8 cut(s)	258, 479, 574, 903, 1083, 1184, 1294, 1653
BmsI	GCATC	4 cut(s)	541, 622, 1154, 1253
BmtI	GCTAGC	1 cut(s)	1017
BpiI	GAAGAC	2 cut(s)	48, 1654
BpmI	CTGGAG	1 cut(s)	213
BpuEI	CTTGAG	1 cut(s)	1856
BpuMI	CCSGG	8 cut(s)	36, 68, 662, 716, 735, 906, 941, 1094
BsaAI	YACGTR	1 cut(s)	474
BsaBI	GATNNNNATC	1 cut(s)	204
BsaHI	GRCGYC	1 cut(s)	1608
BsaI	GGTCTC	2 cut(s)	310, 1199
BsaJI	CCNNGG	4 cut(s)	66, 252, 747, 1262
BsaWI	WCCGGW	4 cut(s)	56, 445, 779, 830
BsaXI	ACNNNNNCTCC	2 cut(s)	302, 332
Bse118I	RCCGGY	4 cut(s)	301, 830, 1289, 1312
Bse1I	ACTGG	3 cut(s)	332, 436, 512
Bse3DI	GCAATG	2 cut(s)	534, 1539
Bse8I	GATNNNNATC	1 cut(s)	204
BseAI	TCCGGA	1 cut(s)	445
BseBI	CCWGG	2 cut(s)	88, 253
BseDI	CCNNGG	4 cut(s)	66, 252, 747, 1262
BseGI	GGATG	4 cut(s)	150, 211, 396, 1705
BseJI	GATNNNNATC	1 cut(s)	204
BseMI	GCAATG	2 cut(s)	534, 1539
BseMII	CTCAG	3 cut(s)	230, 1551, 1905
BseNI	ACTGG	3 cut(s)	332, 436, 512
BseRI	GAGGAG	4 cut(s)	717, 1079, 1279, 1343
BseXI	GCAGC	2 cut(s)	1829, 1894
BshFI	GGCC	7 cut(s)	162, 287, 455, 480, 665, 1312, 1865
BshNI	GGYRCC	2 cut(s)	1081, 1292
BshTI	ACCGGT	1 cut(s)	830
BsiHKAI	GWGCWC	1 cut(s)	318
BsiHKCI	CYCGRG	2 cut(s)	706, 988
BslFI	GGGAC	3 cut(s)	635, 1066, 1619
BsmAI	GTCTC	6 cut(s)	80, 286, 310, 1199, 1457, 1544
BsmFI	GGGAC	3 cut(s)	635, 1066, 1619
BsnI	GGCC	7 cut(s)	162, 287, 455, 480, 665, 1312, 1865
Bso31I	GGTCTC	2 cut(s)	310, 1199
BsoBI	CYCGRG	2 cut(s)	706, 988
Bsp1286I	GDGCHC	1 cut(s)	318
Bsp13I	TCCGGA	1 cut(s)	445
Bsp1407I	TGTACA	1 cut(s)	612
BspACI	CCGC	9 cut(s)	164, 173, 299, 570, 576, 635, 731, 899, 1491
BspANI	GGCC	7 cut(s)	162, 287, 455, 480, 665, 1312, 1865
BspCNI	CTCAG	3 cut(s)	229, 1552, 1904
BspEI	TCCGGA	1 cut(s)	445
BspLI	GGNNCC	8 cut(s)	258, 479, 574, 903, 1083, 1184, 1294, 1653
BspOI	GCTAGC	1 cut(s)	1017
BspPI	GGATC	7 cut(s)	78, 496, 671, 1177, 1190, 1205, 1297
BspT107I	GGYRCC	2 cut(s)	1081, 1292
BspTNI	GGTCTC	2 cut(s)	310, 1199
BsrBI	CCGCTC	1 cut(s)	570
BsrDI	GCAATG	2 cut(s)	534, 1539
BsrFI	RCCGGY	4 cut(s)	301, 830, 1289, 1312
BsrGI	TGTACA	1 cut(s)	612
BsrI	ACTGG	3 cut(s)	332, 436, 512
BssAI	RCCGGY	4 cut(s)	301, 830, 1289, 1312
BssECI	CCNNGG	4 cut(s)	66, 252, 747, 1262
BssNI	GRCGYC	1 cut(s)	1608
BssSI	CACGAG	1 cut(s)	1785
Bst2BI	CACGAG	1 cut(s)	1785
Bst2UI	CCWGG	2 cut(s)	88, 253
Bst4CI	ACNGT	8 cut(s)	398, 596, 628, 1346, 1384, 1449, 1468, 1774
Bst6I	CTCTTC	1 cut(s)	1314
BstACI	GRCGYC	1 cut(s)	1608
BstAUI	TGTACA	1 cut(s)	612
BstBAI	YACGTR	1 cut(s)	474
BstC8I	GCNNGC	3 cut(s)	164, 897, 1015
BstDEI	CTNAG	4 cut(s)	216, 1560, 1647, 1891
BstDSI	CCRYGG	1 cut(s)	747
BstEII	GGTNACC	1 cut(s)	710
BstF5I	GGATG	4 cut(s)	150, 211, 396, 1705
BstHHI	GCGC	3 cut(s)	801, 1628, 1759
BstMAI	GTCTC	6 cut(s)	80, 286, 310, 1199, 1457, 1544
BstMWI	GCNNNNNNNGC	5 cut(s)	172, 533, 567, 662, 798
BstNI	CCWGG	2 cut(s)	88, 253
BstNSI	RCATGY	2 cut(s)	899, 1004
BstPI	GGTNACC	1 cut(s)	710
BstSFI	CTRYAG	1 cut(s)	1500
BstV1I	GCAGC	2 cut(s)	1829, 1894
BstV2I	GAAGAC	2 cut(s)	48, 1654
BstX2I	RGATCY	1 cut(s)	1182
BstYI	RGATCY	1 cut(s)	1182
BsuRI	GGCC	7 cut(s)	162, 287, 455, 480, 665, 1312, 1865
BtgI	CCRYGG	1 cut(s)	747
BtsCI	GGATG	4 cut(s)	150, 211, 396, 1705
BtsI	GCAGTG	1 cut(s)	1483
BtsIMutI	CAGTG	3 cut(s)	505, 1363, 1483
Cac8I	GCNNGC	3 cut(s)	164, 897, 1015
CaiI	CAGNNNCTG	1 cut(s)	1466
CfoI	GCGC	3 cut(s)	801, 1628, 1759
Cfr10I	RCCGGY	4 cut(s)	301, 830, 1289, 1312
CspAI	ACCGGT	1 cut(s)	830
CviAII	CATG	3 cut(s)	896, 1001, 1077
DdeI	CTNAG	4 cut(s)	216, 1560, 1647, 1891
DraIII	CACNNNGTG	1 cut(s)	774
EaeI	YGGCCR	4 cut(s)	285, 453, 663, 1310
Eam1104I	CTCTTC	1 cut(s)	1314
EarI	CTCTTC	1 cut(s)	1314
EciI	GGCGGA	2 cut(s)	565, 720
Ecl136II	GAGCTC	1 cut(s)	316
Eco24I	GRGCYC	1 cut(s)	318
Eco31I	GGTCTC	2 cut(s)	310, 1199
Eco47I	GGWCC	8 cut(s)	59, 330, 398, 496, 573, 777, 782, 1520
Eco53kI	GAGCTC	1 cut(s)	316
Eco57I	CTGAAG	4 cut(s)	219, 1028, 1339, 1620
Eco72I	CACGTG	1 cut(s)	474
Eco88I	CYCGRG	2 cut(s)	706, 988
Eco91I	GGTNACC	1 cut(s)	710
EcoICRI	GAGCTC	1 cut(s)	316
EcoO65I	GGTNACC	1 cut(s)	710
EcoRII	CCWGG	2 cut(s)	86, 251
EcoT22I	ATGCAT	1 cut(s)	1147
EcoT38I	GRGCYC	1 cut(s)	318
FaeI	CATG	3 cut(s)	899, 1004, 1080
FalI	AAGNNNNNCTT	4 cut(s)	875, 907, 1001, 1033
FaqI	GGGAC	3 cut(s)	635, 1066, 1619
FatI	CATG	3 cut(s)	895, 1000, 1076
FauI	CCCGC	3 cut(s)	171, 306, 563
FauNDI	CATATG	1 cut(s)	458
FbaI	TGATCA	1 cut(s)	1732
Fnu4HI	GCNGC	2 cut(s)	1818, 1883
FokI	GGATG	4 cut(s)	137, 198, 403, 1712
FriOI	GRGCYC	1 cut(s)	318
Fsp4HI	GCNGC	2 cut(s)	1818, 1883
FspBI	CTAG	7 cut(s)	9, 275, 689, 1014, 1367, 1394, 1719
FspI	TGCGCA	2 cut(s)	1627, 1758
GlaI	GCGC	3 cut(s)	800, 1627, 1758
GluI	GCNGC	2 cut(s)	1818, 1883
GsuI	CTGGAG	1 cut(s)	213
HaeIII	GGCC	7 cut(s)	162, 287, 455, 480, 665, 1312, 1865
HhaI	GCGC	3 cut(s)	801, 1628, 1759
Hin1I	GRCGYC	1 cut(s)	1608
Hin1II	CATG	3 cut(s)	899, 1004, 1080
Hin6I	GCGC	3 cut(s)	799, 1626, 1757
HinP1I	GCGC	3 cut(s)	799, 1626, 1757
HincII	GTYRAC	2 cut(s)	222, 723
HindII	GTYRAC	2 cut(s)	222, 723
HinfI	GANTC	7 cut(s)	279, 449, 724, 1217, 1454, 1552, 1924
HphI	GGTGA	4 cut(s)	416, 1715, 1786, 1895
Hpy166II	GTNNAC	5 cut(s)	222, 615, 723, 777, 847
Hpy188III	TCNNGA	6 cut(s)	446, 1006, 1100, 1655, 1785, 1873
Hpy8I	GTNNAC	5 cut(s)	222, 615, 723, 777, 847
Hpy99I	CGWCG	6 cut(s)	84, 250, 314, 496, 596, 632
HpyAV	CCTTC	8 cut(s)	85, 262, 877, 955, 1003, 1173, 1595, 1632
HpyCH4III	ACNGT	8 cut(s)	398, 596, 628, 1346, 1384, 1449, 1468, 1774
HpyCH4IV	ACGT	4 cut(s)	245, 473, 974, 1608
HpyCH4V	TGCA	6 cut(s)	895, 927, 1046, 1145, 1544, 1844
HpyF10VI	GCNNNNNNNGC	5 cut(s)	172, 533, 567, 662, 798
HpyF3I	CTNAG	4 cut(s)	216, 1560, 1647, 1891
HpySE526I	ACGT	4 cut(s)	245, 473, 974, 1608
Hsp92I	GRCGYC	1 cut(s)	1608
Hsp92II	CATG	3 cut(s)	899, 1004, 1080
HspAI	GCGC	3 cut(s)	799, 1626, 1757
Kpn2I	TCCGGA	1 cut(s)	445
KpnI	GGTACC	1 cut(s)	1085
Ksp22I	TGATCA	1 cut(s)	1732
LmnI	GCTCC	6 cut(s)	31, 256, 567, 690, 1657, 1814
Lsp1109I	GCAGC	2 cut(s)	1829, 1894
LweI	GCATC	4 cut(s)	541, 622, 1154, 1253
MaeI	CTAG	7 cut(s)	9, 275, 689, 1014, 1367, 1394, 1719
MaeII	ACGT	4 cut(s)	245, 473, 974, 1608
MaeIII	GTNAC	3 cut(s)	710, 758, 1609
MbiI	CCGCTC	1 cut(s)	570
MboII	GAAGA	5 cut(s)	53, 100, 1146, 1331, 1654
MfeI	CAATTG	1 cut(s)	1740
MflI	RGATCY	1 cut(s)	1182
MhlI	GDGCHC	1 cut(s)	318
MluCI	AATT	5 cut(s)	385, 1297, 1439, 1591, 1740
MlyI	GAGTC	4 cut(s)	288, 443, 718, 1546
MmeI	TCCRAC	8 cut(s)	103, 121, 333, 1175, 1398, 1431, 1677, 1820
Mph1103I	ATGCAT	1 cut(s)	1147
MroI	TCCGGA	1 cut(s)	445
MroXI	GAANNNNTTC	1 cut(s)	95
MseI	TTAA	1 cut(s)	1860
MslI	CAYNNNNRTG	2 cut(s)	1031, 1427
MunI	CAATTG	1 cut(s)	1740
MvaI	CCWGG	2 cut(s)	88, 253
MwoI	GCNNNNNNNGC	5 cut(s)	172, 533, 567, 662, 798
NciI	CCSGG	8 cut(s)	36, 68, 662, 716, 735, 906, 941, 1094
NdeI	CATATG	1 cut(s)	458
NheI	GCTAGC	1 cut(s)	1013
NlaIII	CATG	3 cut(s)	899, 1004, 1080
NlaIV	GGNNCC	8 cut(s)	258, 479, 574, 903, 1083, 1184, 1294, 1653
NmeAIII	GCCGAG	3 cut(s)	263, 285, 431
NmuCI	GTSAC	2 cut(s)	758, 1609
NsbI	TGCGCA	2 cut(s)	1627, 1758
NsiI	ATGCAT	1 cut(s)	1147
NspI	RCATGY	2 cut(s)	899, 1004
OliI	CACNNNNGTG	1 cut(s)	1031
PaeI	GCATGC	1 cut(s)	899
PcsI	WCGNNNNNNNCGW	2 cut(s)	290, 600
PdmI	GAANNNNTTC	1 cut(s)	95
PfeI	GAWTC	3 cut(s)	1217, 1454, 1924
PflFI	GACNNNGTC	1 cut(s)	626
PflMI	CCANNNNNTGG	1 cut(s)	774
PfoI	TCCNGGA	1 cut(s)	86
PinAI	ACCGGT	1 cut(s)	830
PkrI	GCNGC	2 cut(s)	1819, 1884
PleI	GAGTC	4 cut(s)	287, 443, 718, 1546
PmaCI	CACGTG	1 cut(s)	474
PmlI	CACGTG	1 cut(s)	474
PpsI	GAGTC	4 cut(s)	287, 443, 718, 1546
Ppu21I	YACGTR	1 cut(s)	474
Psp124BI	GAGCTC	1 cut(s)	318
Psp6I	CCWGG	2 cut(s)	86, 251
PspCI	CACGTG	1 cut(s)	474
PspEI	GGTNACC	1 cut(s)	710
PspGI	CCWGG	2 cut(s)	86, 251
PspN4I	GGNNCC	8 cut(s)	258, 479, 574, 903, 1083, 1184, 1294, 1653
PsrI	GAACNNNNNNTAC	4 cut(s)	413, 445, 1762, 1794
PstNI	CAGNNNCTG	1 cut(s)	1466
PsuI	RGATCY	1 cut(s)	1182
PsyI	GACNNNGTC	1 cut(s)	626
RseI	CAYNNNNRTG	2 cut(s)	1031, 1427
SacI	GAGCTC	1 cut(s)	318
SaqAI	TTAA	1 cut(s)	1860
SatI	GCNGC	2 cut(s)	1818, 1883
ScaI	AGTACT	2 cut(s)	13, 704
SchI	GAGTC	4 cut(s)	288, 443, 718, 1546
SduI	GDGCHC	1 cut(s)	318
SfaNI	GCATC	4 cut(s)	541, 622, 1154, 1253
SfcI	CTRYAG	1 cut(s)	1500
SinI	GGWCC	8 cut(s)	59, 330, 398, 496, 573, 777, 782, 1520
SmiMI	CAYNNNNRTG	2 cut(s)	1031, 1427
SmlI	CTYRAG	1 cut(s)	1871
SmoI	CTYRAG	1 cut(s)	1871
SpeI	ACTAGT	1 cut(s)	8
SphI	GCATGC	1 cut(s)	899
Sse9I	AATT	5 cut(s)	385, 1297, 1439, 1591, 1740
SsiI	CCGC	9 cut(s)	164, 173, 299, 570, 576, 635, 731, 899, 1491
SspMI	CTAG	7 cut(s)	9, 275, 689, 1014, 1367, 1394, 1719
SstI	GAGCTC	1 cut(s)	318
TaaI	ACNGT	8 cut(s)	398, 596, 628, 1346, 1384, 1449, 1468, 1774
TaiI	ACGT	4 cut(s)	248, 476, 977, 1611
TaqI	TCGA	6 cut(s)	293, 491, 630, 881, 959, 1326
TaqII	GACCGA	1 cut(s)	1223
TasI	AATT	5 cut(s)	385, 1297, 1439, 1591, 1740
TatI	WGTACW	4 cut(s)	11, 438, 612, 702
TfiI	GAWTC	3 cut(s)	1217, 1454, 1924
Tru1I	TTAA	1 cut(s)	1860
Tru9I	TTAA	1 cut(s)	1860
TscAI	CASTG	3 cut(s)	512, 1363, 1483
TseFI	GTSAC	2 cut(s)	758, 1609
TseI	GCWGC	2 cut(s)	1817, 1882
Tsp45I	GTSAC	2 cut(s)	758, 1609
TspDTI	ATGAA	4 cut(s)	216, 407, 1086, 1888
TspGWI	ACGGA	7 cut(s)	106, 397, 509, 746, 764, 967, 1827
TspRI	CASTG	3 cut(s)	512, 1363, 1483
Tth111I	GACNNNGTC	1 cut(s)	626
Van91I	CCANNNNNTGG	1 cut(s)	774
VpaK11BI	GGWCC	8 cut(s)	59, 330, 398, 496, 573, 777, 782, 1520
XceI	RCATGY	2 cut(s)	899, 1004
XcmI	CCANNNNNNNNNTGG	1 cut(s)	384
XmnI	GAANNNNTTC	1 cut(s)	95
XspI	CTAG	7 cut(s)	9, 275, 689, 1014, 1367, 1394, 1719
ZraI	GACGTC	1 cut(s)	1609
ZrmI	AGTACT	2 cut(s)	13, 704
Zsp2I	ATGCAT	1 cut(s)	1147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.