Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0019292

Physical Location & Seq

Genomic Coordinates Forward (+)

32614019 .. 32618002

Gene Structure 3984 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1260100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 771 bp

ATGTTATCAGTAATGAATTTTACTTCCAGATGTATTGTGATGATCTTCCAGTCTAGGGGTACATCCGAAAAGTTGAAGATGAGAGCTGGGAAAAAGGGACCAAAAGATATTGATATTGACGTTGAGTTCACTTATTCGGTTAACTGGAAAGCCACATCAACTCAGTTCCAGAACAGGATGGACAAGTACTCAAAGGCTTCATTAATGCCAGTCCGCCAGAAAATTCATTGGTTGTCATTCATTATTATCATCATTGTGCTTTTGATGGGATTGCTTGCTTTGCTCATAGTACGCCGCCTCAAGAATGAGATGAAAAAGTTCTCAAACAGTGACGAAGAAGAAGACAAGGAGGTTGGCTGGAAATACATTCATGGTGATGTTTTCAGATATCCCCCAAACATGTCATTGTTTTGTGCTGTCTTGGGCGTGGGTACCCAACTGCTTGCCATGCTGTTTATCTTATTTTTGCTAGCATTTTTTGGAGTCCTATATCTGAATTTGGGTAAAAGACCCAGAGTGCAGGAGGGACAGGTGGGACAGGTGGTGAAAGATGCAGGAGCCATTGGTCTGATTTTGGCATTCACAGCTGCTAATGCAGAACGAATGTCTGTTTTGACTCTTCAAACAACTGTCGCTGATGTTGGCTCTGCGGTTTCGAATTACCACACCATAGTTTCACAGTCCAGAGGTGCTTATGTGAAAGTCGAGCCTCAGACGTTTAACTTCACCAGAGCAAACGAAAAGCTTTCTCATAAGATCACCTTACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007155 GO:0007165 GO:0008104 GO:0008150 GO:0008283 GO:0009306 GO:0009605 GO:0009607 GO:0009624 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0022610 GO:0023052 GO:0030587 GO:0031224 GO:0031226 GO:0031589 GO:0032502 GO:0032940 GO:0033036 GO:0033299 GO:0034613 GO:0038023 GO:0042886 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0045184 GO:0046903 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051703 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072657 GO:0090702 GO:0098657 GO:0099120

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

257

Amino Acids

28.87

Weight (kDa)

9.74

Isoelectric Point (pI)

29.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	35 - 164	5.6e-38	Endomembrane protein 70
fn3_6	PF17766	198 - 255	7.6e-08	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019292)

Species	Orthologous Gene IDs
malus_domestica	MD07G1260100.v1.1
pyrus_communis	pycom07g23470 pycom07g23790
rosa_chinensis	RchiOBHm_Chr5g0063431 RchiOBHm_Chr6g0273231
rosa_roxburghii	Rroxscaffold_6G00415670
rosa_samantha	Rh1DG035500 Rh5AG393000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	431
AccB1I	GGYRCC	1 cut(s)	431
AciI	CCGC	3 cut(s)	214, 295, 650
AcsI	RAATTY	3 cut(s)	16, 222, 496
AfaI	GTAC	4 cut(s)	61, 188, 291, 433
AfiI	CCNNNNNNNGG	1 cut(s)	55
AflIII	ACRYGT	1 cut(s)	399
AgsI	TTSAA	2 cut(s)	76, 623
AluBI	AGCT	3 cut(s)	86, 587, 745
AluI	AGCT	3 cut(s)	86, 587, 745
ApeKI	GCWGC	1 cut(s)	587
ApoI	RAATTY	3 cut(s)	16, 222, 496
AseI	ATTAAT	1 cut(s)	203
Asp700I	GAANNNNTTC	1 cut(s)	317
Asp718I	GGTACC	1 cut(s)	431
AspS9I	GGNCC	1 cut(s)	98
AsuHPI	GGTGA	4 cut(s)	386, 556, 718, 751
AsuII	TTCGAA	1 cut(s)	656
AsuNHI	GCTAGC	1 cut(s)	469
AvaII	GGWCC	1 cut(s)	98
BanI	GGYRCC	1 cut(s)	431
BbsI	GAAGAC	1 cut(s)	348
BbvI	GCAGC	1 cut(s)	574
BccI	CCATC	2 cut(s)	172, 259
BfaI	CTAG	2 cut(s)	54, 470
BisI	GCNGC	2 cut(s)	295, 588
BlsI	GCNGC	2 cut(s)	296, 589
BmcAI	AGTACT	1 cut(s)	188
Bme18I	GGWCC	1 cut(s)	98
BmgT120I	GGNCC	1 cut(s)	98
BmiI	GGNNCC	3 cut(s)	99, 433, 559
BmsI	GCATC	1 cut(s)	541
BmtI	GCTAGC	1 cut(s)	473
BpiI	GAAGAC	1 cut(s)	348
Bpu14I	TTCGAA	1 cut(s)	656
BpuEI	CTTGAG	1 cut(s)	284
Bsc4I	CCNNNNNNNGG	1 cut(s)	55
Bse1I	ACTGG	3 cut(s)	49, 149, 209
BseGI	GGATG	2 cut(s)	62, 183
BseLI	CCNNNNNNNGG	1 cut(s)	55
BseMII	CTCAG	2 cut(s)	176, 725
BseNI	ACTGG	3 cut(s)	49, 149, 209
BseXI	GCAGC	1 cut(s)	574
BseYI	CCCAGC	1 cut(s)	86
BsgI	GTGCAG	1 cut(s)	539
BshNI	GGYRCC	1 cut(s)	431
BslFI	GGGAC	3 cut(s)	111, 540, 549
BslI	CCNNNNNNNGG	1 cut(s)	55
BsmFI	GGGAC	3 cut(s)	111, 540, 549
BsmI	GAATGC	1 cut(s)	578
Bsp119I	TTCGAA	1 cut(s)	656
Bsp143I	GATC	2 cut(s)	42, 756
BspACI	CCGC	3 cut(s)	214, 295, 650
BspCNI	CTCAG	2 cut(s)	175, 724
BspLI	GGNNCC	3 cut(s)	99, 433, 559
BspOI	GCTAGC	1 cut(s)	473
BspT104I	TTCGAA	1 cut(s)	656
BspT107I	GGYRCC	1 cut(s)	431
BsrI	ACTGG	3 cut(s)	49, 149, 209
BssMI	GATC	2 cut(s)	42, 756
Bst4CI	ACNGT	3 cut(s)	329, 631, 681
Bst6I	CTCTTC	1 cut(s)	624
BstBI	TTCGAA	1 cut(s)	656
BstC8I	GCNNGC	3 cut(s)	276, 444, 471
BstDEI	CTNAG	2 cut(s)	162, 711
BstF5I	GGATG	2 cut(s)	62, 183
BstKTI	GATC	2 cut(s)	45, 759
BstMBI	GATC	2 cut(s)	42, 756
BstMWI	GCNNNNNNNGC	4 cut(s)	280, 448, 584, 593
BstNSI	RCATGY	1 cut(s)	403
BstV1I	GCAGC	1 cut(s)	574
BstV2I	GAAGAC	1 cut(s)	348
BtsCI	GGATG	2 cut(s)	62, 183
BtsIMutI	CAGTG	1 cut(s)	334
Cac8I	GCNNGC	3 cut(s)	276, 444, 471
Cfr13I	GGNCC	1 cut(s)	98
Csp6I	GTAC	4 cut(s)	60, 187, 290, 432
CviAII	CATG	4 cut(s)	371, 400, 448, 768
CviJI	RGCY	9 cut(s)	86, 152, 197, 357, 560, 587, 645, 709, 745
CviKI_1	RGCY	9 cut(s)	86, 152, 197, 357, 560, 587, 645, 709, 745
CviQI	GTAC	4 cut(s)	60, 187, 290, 432
DdeI	CTNAG	2 cut(s)	162, 711
DpnI	GATC	2 cut(s)	44, 758
DpnII	GATC	2 cut(s)	42, 756
Eam1104I	CTCTTC	1 cut(s)	624
EarI	CTCTTC	1 cut(s)	624
EciI	GGCGGA	1 cut(s)	203
Eco32I	GATATC	1 cut(s)	389
Eco47I	GGWCC	1 cut(s)	98
EcoRV	GATATC	1 cut(s)	389
FaeI	CATG	4 cut(s)	374, 403, 451, 771
FaiI	YATR	9 cut(s)	287, 372, 401, 449, 490, 671, 696, 753, 769
FalI	AAGNNNNNCTT	1 cut(s)	746
FaqI	GGGAC	3 cut(s)	111, 540, 549
FatI	CATG	4 cut(s)	370, 399, 447, 767
Fnu4HI	GCNGC	2 cut(s)	295, 588
FokI	GGATG	2 cut(s)	49, 190
Fsp4HI	GCNGC	2 cut(s)	295, 588
FspBI	CTAG	2 cut(s)	54, 470
GluI	GCNGC	2 cut(s)	295, 588
GsaI	CCCAGC	1 cut(s)	90
Hin1II	CATG	4 cut(s)	374, 403, 451, 771
HincII	GTYRAC	1 cut(s)	142
HindII	GTYRAC	1 cut(s)	142
HindIII	AAGCTT	1 cut(s)	743
HinfI	GANTC	2 cut(s)	483, 616
HpaI	GTTAAC	1 cut(s)	142
HphI	GGTGA	4 cut(s)	386, 556, 718, 751
Hpy166II	GTNNAC	2 cut(s)	129, 142
Hpy188I	TCNGA	5 cut(s)	67, 386, 495, 570, 714
Hpy188III	TCNNGA	4 cut(s)	27, 169, 301, 684
Hpy8I	GTNNAC	2 cut(s)	129, 142
HpyCH4III	ACNGT	3 cut(s)	329, 631, 681
HpyCH4IV	ACGT	2 cut(s)	120, 716
HpyCH4V	TGCA	3 cut(s)	520, 554, 596
HpyF10VI	GCNNNNNNNGC	4 cut(s)	280, 448, 584, 593
HpyF3I	CTNAG	2 cut(s)	162, 711
HpySE526I	ACGT	2 cut(s)	120, 716
Hsp92II	CATG	4 cut(s)	374, 403, 451, 771
KpnI	GGTACC	1 cut(s)	435
KspAI	GTTAAC	1 cut(s)	142
Kzo9I	GATC	2 cut(s)	42, 756
LmnI	GCTCC	1 cut(s)	557
Lsp1109I	GCAGC	1 cut(s)	574
LweI	GCATC	1 cut(s)	541
MaeI	CTAG	2 cut(s)	54, 470
MaeII	ACGT	2 cut(s)	120, 716
MaeIII	GTNAC	1 cut(s)	329
MalI	GATC	2 cut(s)	44, 758
MboI	GATC	2 cut(s)	42, 756
MboII	GAAGA	6 cut(s)	37, 88, 347, 350, 353, 611
MluCI	AATT	4 cut(s)	16, 222, 496, 658
MlyI	GAGTC	2 cut(s)	492, 610
MnlI	CCTC	5 cut(s)	308, 343, 517, 680, 720
MroXI	GAANNNNTTC	1 cut(s)	317
MseI	TTAA	3 cut(s)	141, 203, 720
MslI	CAYNNNNRTG	2 cut(s)	254, 375
MspA1I	CMGCKG	1 cut(s)	587
Mva1269I	GAATGC	1 cut(s)	578
MwoI	GCNNNNNNNGC	4 cut(s)	280, 448, 584, 593
NdeII	GATC	2 cut(s)	42, 756
NheI	GCTAGC	1 cut(s)	469
NlaIII	CATG	4 cut(s)	374, 403, 451, 771
NlaIV	GGNNCC	3 cut(s)	99, 433, 559
NmuCI	GTSAC	1 cut(s)	329
NspI	RCATGY	1 cut(s)	403
NspV	TTCGAA	1 cut(s)	656
PciI	ACATGT	1 cut(s)	399
PctI	GAATGC	1 cut(s)	578
PdmI	GAANNNNTTC	1 cut(s)	317
PkrI	GCNGC	2 cut(s)	296, 589
PleI	GAGTC	2 cut(s)	491, 610
PpsI	GAGTC	2 cut(s)	491, 610
PscI	ACATGT	1 cut(s)	399
PshBI	ATTAAT	1 cut(s)	203
PspFI	CCCAGC	1 cut(s)	86
PspN4I	GGNNCC	3 cut(s)	99, 433, 559
PspPI	GGNCC	1 cut(s)	98
PvuII	CAGCTG	1 cut(s)	587
RsaI	GTAC	4 cut(s)	61, 188, 291, 433
RsaNI	GTAC	4 cut(s)	60, 187, 290, 432
RseI	CAYNNNNRTG	2 cut(s)	254, 375
SaqAI	TTAA	3 cut(s)	141, 203, 720
SatI	GCNGC	2 cut(s)	295, 588
Sau3AI	GATC	2 cut(s)	42, 756
Sau96I	GGNCC	1 cut(s)	98
ScaI	AGTACT	1 cut(s)	188
SchI	GAGTC	2 cut(s)	492, 610
SfaNI	GCATC	1 cut(s)	541
SfuI	TTCGAA	1 cut(s)	656
SinI	GGWCC	1 cut(s)	98
SmiMI	CAYNNNNRTG	2 cut(s)	254, 375
SmlI	CTYRAG	1 cut(s)	299
SmoI	CTYRAG	1 cut(s)	299
Sse9I	AATT	4 cut(s)	16, 222, 496, 658
SsiI	CCGC	3 cut(s)	214, 295, 650
SspMI	CTAG	2 cut(s)	54, 470
TaaI	ACNGT	3 cut(s)	329, 631, 681
TaiI	ACGT	2 cut(s)	123, 719
TaqI	TCGA	2 cut(s)	656, 705
TasI	AATT	4 cut(s)	16, 222, 496, 658
TatI	WGTACW	1 cut(s)	186
TauI	GCSGC	1 cut(s)	297
Tru1I	TTAA	3 cut(s)	141, 203, 720
Tru9I	TTAA	3 cut(s)	141, 203, 720
TscAI	CASTG	1 cut(s)	334
TseFI	GTSAC	1 cut(s)	329
TseI	GCWGC	1 cut(s)	587
Tsp45I	GTSAC	1 cut(s)	329
TspDTI	ATGAA	6 cut(s)	29, 189, 215, 229, 326, 359
TspRI	CASTG	1 cut(s)	334
VpaK11BI	GGWCC	1 cut(s)	98
VspI	ATTAAT	1 cut(s)	203
XapI	RAATTY	3 cut(s)	16, 222, 496
XceI	RCATGY	1 cut(s)	403
XmnI	GAANNNNTTC	1 cut(s)	317
XspI	CTAG	2 cut(s)	54, 470
ZrmI	AGTACT	1 cut(s)	188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.