MD07G1269300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0019783
Physical Location & Seq
Forward (+)
33684905 .. 33685549
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1269300.v1.1.491

Sequence Viewer

Length: 645 bp
ATGTTTTCAATTACCAAATTAAAATTCCCTCACAAGCTCAGTGAGCTTAGAGTTTTATCGCTGCCTTTCAATGAATTTGGTGGCGAAATTCCGAAGGAAATTTGGGGGTTGGGGAAGCTTGAAGTGCTTGATCTCGAGGGGAATTTGTTGAACGGAAATTTGCCCACCCGGTTTTTGGGTTTGAGGACGTTTAGGGTTTTGAATCTTGGGTTTAATAGAATCGGTAGGGAGATTCCTTTTTCGCTTTCAAAATTTGTGGATTTGGAGGTGCTGAATTTGGTAGTTAATGAAGTGAGTGGAACCATTGTCGGTAGAATTGGTGGGGAGACTCCCTTATGTCTCTTATTGTCGATCTGCTATATTATGTGGTTGGATTTTAATTGTTTTTTTTATACGAGCACATGTTTTATAAGGACCGACTCTTGGTGCCCACGTTTTTTGCGAAATTTAGAAGGGATTGAAGGTGAACAATCAACTAATGTTGTCCTCTCCTTGCTCCATTACATCACTCACGCTCTCTTGACATTGTTTGTGAGTGGCTCAACGTGGCCAAAACATTTTCTGAGACATGTGGACGCAGAGAAGACGTTGGCGATTCGATCTGATTCAGTCCTCACTTCCCAAACCAAAAATGAAGTCGAATGA

Protein Analysis

215

Amino Acids

24.33

Weight (kDa)

6.53

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 7 - 103 3.1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019783)

Species Orthologous Gene IDs
malus_domestica MD07G1269300.v1.1
rosa_multiflora Rmu_sc0003342.1_g000051
rosa_roxburghii Rroxscaffold_2G00104420 Rroxscaffold_2G00104430
rosa_rugosa Rorug02G0365600
rosa_samantha Rh2BG426700 Rh6BG210700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 548
AcsI RAATTY 9 cut(s) 23, 74, 87, 99, 142, 157, 251, 274, 445
AfiI CCNNNNNNNGG 2 cut(s) 175, 423
AflIII ACRYGT 2 cut(s) 401, 568
AgsI TTSAA 7 cut(s) 9, 70, 122, 151, 202, 249, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 3 cut(s) 37, 46, 118
AluI AGCT 3 cut(s) 37, 46, 118
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 3 cut(s) 320, 344, 559
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 1 cut(s) 548
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 9 cut(s) 23, 74, 87, 99, 142, 157, 251, 274, 445
ArsI GACNNNNNNTTYG 1 cut(s) 621
AspS9I GGNCC 1 cut(s) 414
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 414
BaeGI GKGCMC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 550
BanI GGYRCC 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 48
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 3 cut(s) 320, 344, 559
BisI GCNGC 1 cut(s) 62
BlsI GCNGC 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 2 cut(s) 301, 428
BmrFI CCNGG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 423
BseLI CCNNNNNNNGG 2 cut(s) 175, 423
BseMII CTCAG 2 cut(s) 52, 554
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 1 cut(s) 48
BshFI GGCC 1 cut(s) 550
BshNI GGYRCC 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 169
BslI CCNNNNNNNGG 2 cut(s) 175, 423
BsmAI GTCTC 3 cut(s) 320, 344, 559
BsnI GGCC 1 cut(s) 550
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 401, 431
Bsp143I GATC 3 cut(s) 130, 351, 599
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 2 cut(s) 51, 555
BspLI GGNNCC 2 cut(s) 301, 428
BspT107I GGYRCC 1 cut(s) 426
BssMI GATC 3 cut(s) 130, 351, 599
BstDEI CTNAG 3 cut(s) 38, 47, 563
BstKTI GATC 3 cut(s) 133, 354, 602
BstMAI GTCTC 3 cut(s) 320, 344, 559
BstMBI GATC 3 cut(s) 130, 351, 599
BstMWI GCNNNNNNNGC 2 cut(s) 43, 124
BstNSI RCATGY 2 cut(s) 405, 572
BstSCI CCNGG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 590
BsuRI GGCC 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 414
CseI GACGC 1 cut(s) 584
CspCI CAANNNNNGTGG 4 cut(s) 237, 272, 420, 455
CviAII CATG 2 cut(s) 402, 569
CviJI RGCY 5 cut(s) 37, 46, 118, 540, 550
CviKI_1 RGCY 5 cut(s) 37, 46, 118, 540, 550
DdeI CTNAG 3 cut(s) 38, 47, 563
DpnI GATC 3 cut(s) 132, 353, 601
DpnII GATC 3 cut(s) 130, 351, 599
EaeI YGGCCR 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 134
FaeI CATG 2 cut(s) 405, 572
FaiI YATR 7 cut(s) 337, 360, 365, 393, 403, 410, 570
FatI CATG 2 cut(s) 401, 568
Fnu4HI GCNGC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 62
GluI GCNGC 1 cut(s) 62
HaeIII GGCC 1 cut(s) 550
HapII CCGG 1 cut(s) 169
HgaI GACGC 1 cut(s) 584
Hin1II CATG 2 cut(s) 405, 572
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 7 cut(s) 202, 219, 232, 328, 419, 595, 605
HpaII CCGG 1 cut(s) 169
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 2 cut(s) 467, 574
Hpy188I TCNGA 3 cut(s) 93, 564, 604
Hpy188III TCNNGA 2 cut(s) 134, 520
Hpy8I GTNNAC 2 cut(s) 467, 574
HpyAV CCTTC 3 cut(s) 88, 446, 455
HpyCH4IV ACGT 4 cut(s) 188, 433, 545, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 124
HpyF3I CTNAG 3 cut(s) 38, 47, 563
HpySE526I ACGT 4 cut(s) 188, 433, 545, 587
Hsp92II CATG 2 cut(s) 405, 572
Kzo9I GATC 3 cut(s) 130, 351, 599
LmnI GCTCC 1 cut(s) 501
LpnPI CCDG 1 cut(s) 182
Lsp1109I GCAGC 1 cut(s) 48
MaeII ACGT 4 cut(s) 188, 433, 545, 587
MalI GATC 3 cut(s) 132, 353, 601
MboI GATC 3 cut(s) 130, 351, 599
MboII GAAGA 1 cut(s) 595
MhlI GDGCHC 2 cut(s) 401, 431
MlsI TGGCCA 1 cut(s) 550
MluNI TGGCCA 1 cut(s) 550
MlyI GAGTC 2 cut(s) 322, 413
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 6 cut(s) 39, 130, 177, 259, 497, 623
Mox20I TGGCCA 1 cut(s) 550
MscI TGGCCA 1 cut(s) 550
MseI TTAA 4 cut(s) 20, 213, 285, 378
Msp20I TGGCCA 1 cut(s) 550
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 169
MwoI GCNNNNNNNGC 2 cut(s) 43, 124
NciI CCSGG 1 cut(s) 169
NdeII GATC 3 cut(s) 130, 351, 599
NlaIII CATG 2 cut(s) 405, 572
NlaIV GGNNCC 2 cut(s) 301, 428
NspI RCATGY 2 cut(s) 405, 572
PaeR7I CTCGAG 1 cut(s) 134
PciI ACATGT 2 cut(s) 401, 568
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PfeI GAWTC 5 cut(s) 202, 219, 232, 595, 605
PkrI GCNGC 1 cut(s) 63
PleI GAGTC 2 cut(s) 322, 413
PpsI GAGTC 2 cut(s) 322, 413
PscI ACATGT 2 cut(s) 401, 568
PsiI TTATAA 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 301, 428
PspPI GGNCC 1 cut(s) 414
SaqAI TTAA 4 cut(s) 20, 213, 285, 378
SatI GCNGC 1 cut(s) 62
Sau3AI GATC 3 cut(s) 130, 351, 599
Sau96I GGNCC 1 cut(s) 414
SchI GAGTC 2 cut(s) 322, 413
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 2 cut(s) 401, 431
SetI ASST 9 cut(s) 39, 48, 120, 191, 270, 436, 466, 548, 590
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 414
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 167
TaiI ACGT 4 cut(s) 191, 436, 548, 590
TaqI TCGA 4 cut(s) 135, 350, 598, 639
TaqII GACCGA 1 cut(s) 431
TfiI GAWTC 5 cut(s) 202, 219, 232, 595, 605
Tru1I TTAA 4 cut(s) 20, 213, 285, 378
Tru9I TTAA 4 cut(s) 20, 213, 285, 378
TscAI CASTG 1 cut(s) 46
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 2 cut(s) 87, 303
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 414
XapI RAATTY 9 cut(s) 23, 74, 87, 99, 142, 157, 251, 274, 445
XceI RCATGY 2 cut(s) 405, 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.