MD07G1294900.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009257
Physical Location & Seq
Reverse (-)
35558276 .. 35563080
4805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1294900.v1.1.491

Sequence Viewer

Length: 2172 bp
ATGGGTAGCTTTAGTGAAGAGGAAGAAGATTGTGGATTTTTTGATGCACAAGAGGAAATTGCATCAACATCCGATGCAGGGTCTGAAACCGTTGAGATATTTGATTCCAATTCTTCCTTCACCAATTGGGTTCCAGCTGGTTGTCCGTATGATGTGTGGAATAGAACCCCGGGAAGTGTGAAGGAGCGGCGGGTTAAGTTTCTGGAGTGGATGGGACTGAGTACGGATCGAGAGACTTCAGTGGAAATAGCAAGTGATGTAGCAGGGGAGGTTGAGAGAGTTAGAGAGAGTAGTGGGGCTGTGTTGAGAACCACCTCATGTGTTGAGGATGAGTATTGTTTAAGTCGGTCATTTGTGTCATGTCGGCGTAATGACTACTCCATTTATTCCTCAATTGAGTTGGATTGGAAGGATGATTCTGCATGTAGAGATGGGAATTTAGGGAAGCCATTGGAGTGTAATGTAGATGAAGTGAGGCTGGATGGGAAGGTACTAGAAGGTCAAGAAGTGACCGAAGAGTCCGAGCGCAATTCTTGTTCTTCATCTTCATTGTCTCTACAATTGACGGATAATCGAATGGAGGAGGCAAGCAATAGAGCTGGGATACTGAAGAGAGCTAAGAAGGGGTGGTTGAGTAGGTTGCGGTCCATCGCATGCCTTGTTGATAGACAAGGGGAGGGTGATGAATTGGCACACGATGACAATGCTGCAATCATGGGGTTCAGGGCGCAAAGAGTTAAGGTTCGCCAGTGCGGGAAGCGGTTGAAGGAACTTTCAGCACTTTACATGGGGCAAGATATCCAGGCACATGAGGGCGCAATTTTGGCAATGAAATTCAGTCCTGATGGACAATACTTGGCAAGTGCTGGTGAAGATGGGATTGTGCGTGTCTGGCAAGTGGTGGAGGATGAGAGATCTAATGAACATGACATTCCGGAAATAGATCCATCCTGCATTTACTTCACAGTGAATCATCTCTCTGAACTAAATCCACTATTTTCGGAAAAGGATACAATAGGCAAATCAAGTAGCCTTAGGAAAACATCAGATTCAGCTTGTGTTATTTTCCCCCCAAAAGTCTTCCGGATATTGGAGAAACCGTTGCATGAATTTCACGGGCATGGTGGTGATATCTTGGATCTCTCTTGGTCAAGGAGTAATTATCTTCTGTCATCTTCGGTTGACAAAACTGTTCGTCTTTGGCAAGTGGGATGCGGTAGTTGCCTCAAAGTCTTTTCCCATAGTAATTATGTGACCAGTGTTCAATTTAATCCAGTGGATGAGAATTACTTCATCAGTGGTTCAATAGATGGCAAAGTACGTATTTGGGGAATTTCTTGCTGCCAAGTTGTTGATTGGAGTGATATAAGAGATATAGTTACTGCGGTGTGCTATCGCCCTGATGGGCAGGGAGGAATTGTTGGCTCAATGACAGGCAGTTGCCGCTTTTATAATATATCAGATAATCATTTGCAATTGGATGCTCAATTATGCTTGCAAAGTAAAAAGAAGTCCCCGTGCAGAAAGATTACAGGCTTCCAGTTTTTCCCACAAGACTCTAGCAAAGTGATGGTCACTTGTGCCGATTCAAAAGTCAGAATTCTTCATGGTCTATCTGTGGTCGGCAAGTATGCTGGACAACGAAATGCAGGAAACCAGACATCTGCTACTATCACTTCAGATGGGAAACATATAGTCTCGGTTAATGAGGATTCTAGTGTATATGTATGGAACTGCAATAATCATGAAGAACGAGTTCTCTCTCAAGCAAAAAAGATAAGATCTTGTGAGCGCTTTTCCACGAATGTTTCTGTGGCAATACCTTGGTCTGGATTTAAATGTGGGACCGTAGAAAATGAGAGGCAATTTCAGGCTGTCGATGAGAATTTACCGGAGAGACTTCCTTTTTCCTCACCTGCGTGTTTTTCTCTCAGCCAAGATTCTTTCCTTCAGTCTATTCCCAAGGGATCTGCGACTTGGCCAGAGGAGAAGCTTCCAACTTCGAGCCCACTGGCAAAGCAATCTACTATGCACAAATCCGAGTACAAGTTTCTTAAAACTTCTTGCCAGAACACATCCAGTTCACATGCATGGGGTATGGTTATTGTTACTGCAGGATGGGATGGACGGATCAGATCATTCCACAATTATGGTTTACCAGTAACCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

724

Amino Acids

80.42

Weight (kDa)

5.45

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 235 - 303 5.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 256 - 488 4.8e-15 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 264 - 301 2e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 266 - 311 2.6e-07 WDHD1 first WD40 domain
WD40_Gbeta PF25391 267 - 578 2.5e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 267 - 302 1.7e-06 WDR5 beta-propeller domain
WD40 PF00400 267 - 298 1.3e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 365 - 445 5.7e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 365 - 490 9e-13 Prp19 WD40 domain
WD40 PF00400 366 - 401 4.6e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 366 - 583 1.2e-32 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 368 - 428 9.4e-09 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 369 - 468 7.3e-09 CDC20/Fizzy WD40 domain
EIF3I PF24805 370 - 430 2.8e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 371 - 428 1.9e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 375 - 589 4.3e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 377 - 577 3.7e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 389 - 493 2.1e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 406 - 443 3.9e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 428 - 611 4.4e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 453 - 584 8.1e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1452
AarI CACCTGC 1 cut(s) 1924
AasI GACNNNNNNGTC 1 cut(s) 517
Acc36I ACCTGC 1 cut(s) 1924
AccBSI CCGCTC 1 cut(s) 187
AccIII TCCGGA 2 cut(s) 934, 1083
AciI CCGC 8 cut(s) 187, 190, 643, 753, 760, 1215, 1385, 1444
AclWI GGATC 5 cut(s) 234, 938, 1146, 1975, 2138
AcoI YGGCCR 1 cut(s) 1979
AcsI RAATTY 6 cut(s) 436, 833, 1109, 1332, 1599, 1885
AcuI CTGAAG 4 cut(s) 222, 629, 1662, 1934
AfaI GTAC 4 cut(s) 223, 492, 1320, 2045
AfeI AGCGCT 1 cut(s) 1793
AfiI CCNNNNNNNGG 3 cut(s) 78, 1090, 1829
AgsI TTSAA 4 cut(s) 766, 1265, 1305, 1590
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 1918
AluBI AGCT 6 cut(s) 9, 137, 599, 617, 1055, 1993
AluI AGCT 6 cut(s) 9, 137, 599, 617, 1055, 1993
Alw26I GTCTC 4 cut(s) 227, 558, 1702, 1891
AlwI GGATC 5 cut(s) 234, 938, 1146, 1975, 2138
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 169
Aor13HI TCCGGA 2 cut(s) 934, 1083
Aor51HI AGCGCT 1 cut(s) 1793
AoxI GGCC 1 cut(s) 1979
ApeKI GCWGC 2 cut(s) 707, 1341
ApoI RAATTY 6 cut(s) 436, 833, 1109, 1332, 1599, 1885
ArsI GACNNNNNNTTYG 2 cut(s) 1557, 1589
Asp700I GAANNNNTTC 2 cut(s) 1289, 1755
AspLEI GCGC 4 cut(s) 528, 730, 818, 1794
AspS9I GGNCC 2 cut(s) 645, 1845
AsuC2I CCSGG 2 cut(s) 170, 171
AsuHPI GGTGA 5 cut(s) 112, 692, 881, 1139, 1905
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 2 cut(s) 645, 1845
AxyI CCTNAGG 1 cut(s) 1034
BalI TGGCCA 1 cut(s) 1981
BanII GRGCYC 1 cut(s) 2009
BbsI GAAGAC 1 cut(s) 1072
BbvI GCAGC 2 cut(s) 694, 1328
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 2 cut(s) 597, 1003
BcnI CCSGG 2 cut(s) 170, 171
BcoDI GTCTC 4 cut(s) 227, 558, 1702, 1891
BfaI CTAG 3 cut(s) 494, 1560, 1716
BfmI CTRYAG 1 cut(s) 2112
BfoI RGCGCY 1 cut(s) 1795
BfuAI ACCTGC 1 cut(s) 1924
BfuI GTATCC 2 cut(s) 597, 1003
BglII AGATCT 2 cut(s) 914, 1781
BisI GCNGC 4 cut(s) 188, 708, 1342, 1444
BlsI GCNGC 4 cut(s) 189, 709, 1343, 1445
Bme1390I CCNGG 3 cut(s) 170, 171, 803
Bme18I GGWCC 2 cut(s) 645, 1845
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 645, 1845
BmiI GGNNCC 2 cut(s) 132, 1846
BmrFI CCNGG 3 cut(s) 170, 171, 803
BmsI GCATC 5 cut(s) 34, 64, 71, 1202, 1471
BpiI GAAGAC 1 cut(s) 1072
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 1749
BpuMI CCSGG 2 cut(s) 170, 171
BsaAI YACGTR 1 cut(s) 1322
BsaJI CCNNGG 4 cut(s) 168, 169, 1823, 1962
BsaWI WCCGGW 3 cut(s) 934, 1083, 1891
BsaXI ACNNNNNCTCC 4 cut(s) 1085, 1115, 1404, 1434
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1090, 1829
Bse1I ACTGG 7 cut(s) 748, 1257, 1274, 1540, 2016, 2079, 2159
Bse21I CCTNAGG 1 cut(s) 1034
Bse3DI GCAATG 1 cut(s) 834
BseAI TCCGGA 2 cut(s) 934, 1083
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 4 cut(s) 168, 169, 1823, 1962
BseLI CCNNNNNNNGG 3 cut(s) 78, 1090, 1829
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 2 cut(s) 209, 1945
BseNI ACTGG 7 cut(s) 748, 1257, 1274, 1540, 2016, 2079, 2159
BseRI GAGGAG 2 cut(s) 596, 2000
BseXI GCAGC 2 cut(s) 694, 1328
BseYI CCCAGC 1 cut(s) 599
BsgI GTGCAG 1 cut(s) 1540
BshFI GGCC 1 cut(s) 1981
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 4 cut(s) 170, 935, 1084, 1892
BslFI GGGAC 3 cut(s) 228, 1498, 1858
BslI CCNNNNNNNGG 3 cut(s) 78, 1090, 1829
BsmAI GTCTC 4 cut(s) 227, 558, 1702, 1891
BsmFI GGGAC 3 cut(s) 228, 1498, 1858
BsnI GGCC 1 cut(s) 1981
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 2009
Bsp13I TCCGGA 2 cut(s) 934, 1083
Bsp143I GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
BspACI CCGC 8 cut(s) 187, 190, 643, 753, 760, 1215, 1385, 1444
BspANI GGCC 1 cut(s) 1981
BspCNI CTCAG 2 cut(s) 210, 1944
BspEI TCCGGA 2 cut(s) 934, 1083
BspHI TCATGA 1 cut(s) 1744
BspLI GGNNCC 2 cut(s) 132, 1846
BspMAI CTGCAG 1 cut(s) 2116
BspMI ACCTGC 1 cut(s) 1924
BspPI GGATC 5 cut(s) 234, 938, 1146, 1975, 2138
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 834
BsrI ACTGG 7 cut(s) 748, 1257, 1274, 1540, 2016, 2079, 2159
BssECI CCNNGG 4 cut(s) 168, 169, 1823, 1962
BssMI GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
BssT1I CCWWGG 2 cut(s) 1823, 1962
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 6 cut(s) 91, 967, 1101, 1192, 1849, 2167
Bst6I CTCTTC 3 cut(s) 12, 510, 605
BstBAI YACGTR 1 cut(s) 1322
BstC8I GCNNGC 3 cut(s) 589, 655, 1496
BstDEI CTNAG 4 cut(s) 218, 618, 1034, 1931
BstH2I RGCGCY 1 cut(s) 1795
BstHHI GCGC 4 cut(s) 528, 730, 818, 1794
BstKTI GATC 8 cut(s) 229, 917, 946, 1141, 1784, 1970, 2133, 2138
BstMAI GTCTC 4 cut(s) 227, 558, 1702, 1891
BstMBI GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
BstMWI GCNNNNNNNGC 4 cut(s) 824, 892, 1221, 1443
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 3 cut(s) 426, 657, 2090
BstSCI CCNGG 3 cut(s) 168, 169, 801
BstSFI CTRYAG 1 cut(s) 2112
BstSNI TACGTA 1 cut(s) 1322
BstV1I GCAGC 2 cut(s) 694, 1328
BstV2I GAAGAC 1 cut(s) 1072
BstX2I RGATCY 5 cut(s) 914, 943, 1138, 1781, 1967
BstXI CCANNNNNNTGG 1 cut(s) 2150
BstYI RGATCY 5 cut(s) 914, 943, 1138, 1781, 1967
Bsu36I CCTNAGG 1 cut(s) 1034
BsuI GTATCC 2 cut(s) 597, 1003
BsuRI GGCC 1 cut(s) 1981
BtgZI GCGATG 1 cut(s) 634
BtsIMutI CAGTG 7 cut(s) 246, 755, 972, 1264, 1281, 1303, 2009
BveI ACCTGC 1 cut(s) 1924
Cac8I GCNNGC 3 cut(s) 589, 655, 1496
CaiI CAGNNNCTG 1 cut(s) 83
CciI TCATGA 1 cut(s) 1744
CfoI GCGC 4 cut(s) 528, 730, 818, 1794
Cfr13I GGNCC 2 cut(s) 645, 1845
Cfr9I CCCGGG 1 cut(s) 169
Csp6I GTAC 4 cut(s) 222, 491, 1319, 2044
CviQI GTAC 4 cut(s) 222, 491, 1319, 2044
DdeI CTNAG 4 cut(s) 218, 618, 1034, 1931
DpnI GATC 8 cut(s) 228, 916, 945, 1140, 1783, 1969, 2132, 2137
DpnII GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
DraI TTTAAA 1 cut(s) 1837
DrdI GACNNNNNNGTC 1 cut(s) 517
DseDI GACNNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 1 cut(s) 1979
Eam1104I CTCTTC 3 cut(s) 12, 510, 605
EarI CTCTTC 3 cut(s) 12, 510, 605
Eco105I TACGTA 1 cut(s) 1322
Eco130I CCWWGG 2 cut(s) 1823, 1962
Eco24I GRGCYC 1 cut(s) 2009
Eco32I GATATC 2 cut(s) 799, 1132
Eco47I GGWCC 2 cut(s) 645, 1845
Eco47III AGCGCT 1 cut(s) 1793
Eco57I CTGAAG 4 cut(s) 222, 629, 1662, 1934
Eco81I CCTNAGG 1 cut(s) 1034
Eco88I CYCGRG 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 1599
EcoRII CCWGG 1 cut(s) 801
EcoRV GATATC 2 cut(s) 799, 1132
EcoT14I CCWWGG 2 cut(s) 1823, 1962
EcoT22I ATGCAT 1 cut(s) 2092
EcoT38I GRGCYC 1 cut(s) 2009
ErhI CCWWGG 2 cut(s) 1823, 1962
FaqI GGGAC 3 cut(s) 228, 1498, 1858
FauI CCCGC 2 cut(s) 183, 746
Fnu4HI GCNGC 4 cut(s) 188, 708, 1342, 1444
FriOI GRGCYC 1 cut(s) 2009
Fsp4HI GCNGC 4 cut(s) 188, 708, 1342, 1444
FspBI CTAG 3 cut(s) 494, 1560, 1716
GlaI GCGC 4 cut(s) 527, 729, 817, 1793
GluI GCNGC 4 cut(s) 188, 708, 1342, 1444
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 1795
HaeIII GGCC 1 cut(s) 1981
HapII CCGG 4 cut(s) 170, 935, 1084, 1892
HhaI GCGC 4 cut(s) 528, 730, 818, 1794
Hin6I GCGC 4 cut(s) 526, 728, 816, 1792
HinP1I GCGC 4 cut(s) 526, 728, 816, 1792
HincII GTYRAC 1 cut(s) 1183
HindII GTYRAC 1 cut(s) 1183
HindIII AAGCTT 1 cut(s) 1991
HinfI GANTC 9 cut(s) 104, 416, 518, 970, 1049, 1556, 1586, 1712, 1940
HpaII CCGG 4 cut(s) 170, 935, 1084, 1892
HphI GGTGA 5 cut(s) 112, 692, 881, 1139, 1905
Hpy166II GTNNAC 3 cut(s) 1183, 2084, 2156
Hpy188III TCNNGA 8 cut(s) 203, 230, 503, 842, 935, 1084, 1745, 1830
Hpy8I GTNNAC 3 cut(s) 1183, 2084, 2156
HpyAV CCTTC 8 cut(s) 127, 175, 403, 481, 491, 616, 760, 1958
HpyCH4III ACNGT 6 cut(s) 91, 967, 1101, 1192, 1849, 2167
HpyCH4IV ACGT 1 cut(s) 1321
HpyF10VI GCNNNNNNNGC 4 cut(s) 824, 892, 1221, 1443
HpyF3I CTNAG 4 cut(s) 218, 618, 1034, 1931
HpySE526I ACGT 1 cut(s) 1321
HspAI GCGC 4 cut(s) 526, 728, 816, 1792
Kpn2I TCCGGA 2 cut(s) 934, 1083
Kzo9I GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 694, 1328
LweI GCATC 5 cut(s) 34, 64, 71, 1202, 1471
MaeI CTAG 3 cut(s) 494, 1560, 1716
MaeII ACGT 1 cut(s) 1321
MaeIII GTNAC 6 cut(s) 508, 1252, 1378, 1573, 2107, 2161
MalI GATC 8 cut(s) 228, 916, 945, 1140, 1783, 1969, 2132, 2137
MbiI CCGCTC 1 cut(s) 187
MboI GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
MfeI CAATTG 4 cut(s) 124, 393, 560, 1475
MflI RGATCY 5 cut(s) 914, 943, 1138, 1781, 1967
MhlI GDGCHC 1 cut(s) 2009
MlsI TGGCCA 1 cut(s) 1981
MluNI TGGCCA 1 cut(s) 1981
MlyI GAGTC 2 cut(s) 527, 1550
MmeI TCCRAC 2 cut(s) 381, 2021
Mox20I TGGCCA 1 cut(s) 1981
Mph1103I ATGCAT 1 cut(s) 2092
MroI TCCGGA 2 cut(s) 934, 1083
MroXI GAANNNNTTC 2 cut(s) 1289, 1755
MscI TGGCCA 1 cut(s) 1981
MseI TTAA 7 cut(s) 195, 341, 738, 1269, 1704, 1836, 2055
MslI CAYNNNNRTG 6 cut(s) 454, 1119, 1125, 1918, 2089, 2148
Msp20I TGGCCA 1 cut(s) 1981
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 4 cut(s) 170, 935, 1084, 1892
MspR9I CCNGG 3 cut(s) 170, 171, 803
MunI CAATTG 4 cut(s) 124, 393, 560, 1475
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 4 cut(s) 824, 892, 1221, 1443
NciI CCSGG 2 cut(s) 170, 171
NdeII GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
NlaIV GGNNCC 2 cut(s) 132, 1846
NmuCI GTSAC 3 cut(s) 508, 1252, 1573
NsiI ATGCAT 1 cut(s) 2092
NspI RCATGY 3 cut(s) 426, 657, 2090
OliI CACNNNNGTG 1 cut(s) 1918
PaeI GCATGC 1 cut(s) 657
PagI TCATGA 1 cut(s) 1744
PaqCI CACCTGC 1 cut(s) 1924
PdmI GAANNNNTTC 2 cut(s) 1289, 1755
PfeI GAWTC 7 cut(s) 104, 416, 970, 1049, 1586, 1712, 1940
PkrI GCNGC 4 cut(s) 189, 709, 1343, 1445
PleI GAGTC 2 cut(s) 526, 1550
PpsI GAGTC 2 cut(s) 526, 1550
Ppu21I YACGTR 1 cut(s) 1322
PsiI TTATAA 1 cut(s) 1452
Psp6I CCWGG 1 cut(s) 801
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 2 cut(s) 132, 1846
PspPI GGNCC 2 cut(s) 645, 1845
PstI CTGCAG 1 cut(s) 2116
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 5 cut(s) 914, 943, 1138, 1781, 1967
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 4 cut(s) 223, 492, 1320, 2045
RsaNI GTAC 4 cut(s) 222, 491, 1319, 2044
RseI CAYNNNNRTG 6 cut(s) 454, 1119, 1125, 1918, 2089, 2148
SaqAI TTAA 7 cut(s) 195, 341, 738, 1269, 1704, 1836, 2055
SatI GCNGC 4 cut(s) 188, 708, 1342, 1444
Sau3AI GATC 8 cut(s) 226, 914, 943, 1138, 1781, 1967, 2130, 2135
Sau96I GGNCC 2 cut(s) 645, 1845
SchI GAGTC 2 cut(s) 527, 1550
ScrFI CCNGG 3 cut(s) 170, 171, 803
SduI GDGCHC 1 cut(s) 2009
SfaNI GCATC 5 cut(s) 34, 64, 71, 1202, 1471
SfcI CTRYAG 1 cut(s) 2112
SinI GGWCC 2 cut(s) 645, 1845
SmaI CCCGGG 1 cut(s) 171
SmiI ATTTAAAT 1 cut(s) 1837
SmiMI CAYNNNNRTG 6 cut(s) 454, 1119, 1125, 1918, 2089, 2148
SmlI CTYRAG 1 cut(s) 1764
SmoI CTYRAG 1 cut(s) 1764
SnaBI TACGTA 1 cut(s) 1322
SphI GCATGC 1 cut(s) 657
SsiI CCGC 8 cut(s) 187, 190, 643, 753, 760, 1215, 1385, 1444
SspMI CTAG 3 cut(s) 494, 1560, 1716
StyD4I CCNGG 3 cut(s) 168, 169, 801
StyI CCWWGG 2 cut(s) 1823, 1962
SwaI ATTTAAAT 1 cut(s) 1837
TaaI ACNGT 6 cut(s) 91, 967, 1101, 1192, 1849, 2167
TaiI ACGT 1 cut(s) 1324
TaqI TCGA 4 cut(s) 229, 574, 1878, 2003
TaqII GACCGA 2 cut(s) 336, 527
TatI WGTACW 1 cut(s) 2043
TauI GCSGC 2 cut(s) 190, 1446
TfiI GAWTC 7 cut(s) 104, 416, 970, 1049, 1586, 1712, 1940
Tru1I TTAA 7 cut(s) 195, 341, 738, 1269, 1704, 1836, 2055
Tru9I TTAA 7 cut(s) 195, 341, 738, 1269, 1704, 1836, 2055
TscAI CASTG 7 cut(s) 246, 755, 972, 1264, 1281, 1303, 2016
TseFI GTSAC 3 cut(s) 508, 1252, 1573
TseI GCWGC 2 cut(s) 707, 1341
Tsp45I GTSAC 3 cut(s) 508, 1252, 1573
TspGWI ACGGA 4 cut(s) 135, 239, 581, 2143
TspMI CCCGGG 1 cut(s) 169
TspRI CASTG 7 cut(s) 246, 755, 972, 1264, 1281, 1303, 2016
VpaK11BI GGWCC 2 cut(s) 645, 1845
XapI RAATTY 6 cut(s) 436, 833, 1109, 1332, 1599, 1885
XceI RCATGY 3 cut(s) 426, 657, 2090
XmaI CCCGGG 1 cut(s) 169
XmnI GAANNNNTTC 2 cut(s) 1289, 1755
XspI CTAG 3 cut(s) 494, 1560, 1716
Zsp2I ATGCAT 1 cut(s) 2092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.