Rw1G039620

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009257
Physical Location & Seq
Reverse (-)
67130392 .. 67134794
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G039620.1

Sequence Viewer

Length: 2118 bp
ATGGGGAGCTTTAGCGAAGAGGAATTCGCTTTTTTTGATGCCCGCGAGGATATTGCATCAGTGTCTGATGCAAGTCGTGAGAGCATTGAGGTGTTGGAAGCGAGTTCTTCTTCCATGAGTTGGGTGCCGGATAGTTTTCCCTATGATGTGTGGGTTAGAAGCCCGGGGAGTGTGAAGGAGCGTCGGGTTAAGTTTCTGGATTGGATGGAAGTTGGTGCAGAGGAGATTGTGGGAGAGAATATGGTGGAAGTGTCTAGTGATGTGGTGGGGGAAATAGGTAGAGTTAGAGAGAGTAGTGGGGCTGTGTTGAGATCCTTATCTTTTGAGGGGGAGTTTTCTTCAAGTCGGTCTTCAATGTCGTTCTGGACTAATGAATTTAGAGATGGGAATTTGAGTGGGAGAATGGAGTGTAATGGAGATGAAGTAGGCCCGGTTGGGAAGGTCAGTGAAGGTCGAGAAGTTATTGAAGAGTCTGAGCGCACTTTGTTCTCATTATCTCGTCAATTGAGTGAAGTCAAAGCTGAAGAGATTAGGAGTAAGGGGGCCTTACTGAAGAGAGTTAAGAAGAAGTGGTTGCATAGATTTCGGTCCATGACATGCCTTGATGATAGGCAAGGGATTGCTGACAGGTCGGCAGAGAATGATAATGATAGAATTATGGGAATAAGGTCTTATAGAGTGAAGGTTCGTCATTCCGGGAAGCGGTTGAAGGAACTCTCAGCTCTTTATATGGGACAGGATATCCAGGCCCATGAGGGTTCAATTTTGGCCATGAAATTTAGTCCTGATGGACAGTACCTAGCAAGTGCTGGTGAAGATGGGATTGTGCGAGTCTGGCAAGTGGTGGAGAATCAGAGGTGTAATGAACTTGACATTCCAGAGATAGATCCATCCTGCATATACTTCACCGTGAATCATCTCTCTGAACTAAATCCACTATTTGCGGATAAAGAGAAAGTGAGTAAAGCACGTAGCATGACGAAAACATCAGATTCAGCATGTGTTATTTTTCCCCCAAAAGTCTTTCGGATTGTGGACAAACCATTGCATGAGTTTCATGGGCATAGTGGTGAGATTTTGGATCTCTCCTGGTCAAGGAACAATTATCTACTGTCGTCTTCTGTTGACAAAACTGTTCGTCTATGGCAAGTGGGATGCACTGATTGCCTCAAAGTATTTGCACATAGTAATTATGTGACCTGTGTTCAATTCAATCCTATGGATGATAACTACTTTATCAGTGGTTCAATAGATGGCAAAGTTCGCATTTGGGGAATTCCTTGCTGTCAAGTTGTTGATTATAGCGATGTACGAGATATAGTTTCTGCGGTGTGCTATCGCCCTGATGGGCAGGGAGCTATTGTAGGCTCGATGACAGGCATATGCCGCTTTTATAATACATCAGATAATCATTTGCAAATGGACGCTGAAATCTGTTTGTACAGTAAAAAGAAGGCTCCCTGCAAAAAGATAACAGGCTTCGAGTTTTTCCCACAAGATCCTAGCAAAGTAATGGTCACTTGCGCCGATTCGCAAGTCAGAATTCTTCATGGTCTGAATGTGGTTGGAAAATACAAGGGATTACGGAATGCAGCAAACCTGAGATCTGCGACTTTTACTTCAGATGGGAAACATATAATATCAGCAAGTGAGGATTCTAATGTATACATATGGAACTGCAGTGATCAGGAGAAAAACTTTTTCTCACAAGTAAAAAAAACTAAATCATGTGAGCGGTTCACCACTGATGCTTCCGTAGCAATACCATGGTCTGGTTTGAAATGTGAGGCCTTAGAAAATGCTAGGAACGTTGGTGTCGTGGAAAATAATCTACCTGAAACTCTACCTTTTTCTTCACCCGCATGCTTTTCTCTGAGCCAAGAATCTCTCCTTCAGTCTATTCCGAAAGGATCTGCGACTTGGCCCGAGGAGAAGCTTCCAACATCAAGCGCCTTGACAAAGTCATCTACTATGCACAGATCCGAGTACAAGTTTTTTAAAACTTCATGCCAGAGCACATCGAGTTCCCATGCATGGGGTCTGGTTATTGTGACCGCAGGTTGGGATGGAAGGATCAAATCATTCCACAATTATGGGTTACCAGTACCTGTTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

705

Amino Acids

78.83

Weight (kDa)

6.05

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 226 - 288 1.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 237 - 470 1.6e-15 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 246 - 284 1.1e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 247 - 291 2.5e-07 WDHD1 first WD40 domain
WD40 PF00400 248 - 279 9.5e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 248 - 287 6.9e-07 WDR5 beta-propeller domain
WD40_Gbeta PF25391 331 - 559 5.8e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 341 - 564 2e-35 WDR5 beta-propeller domain
WD40_Prp19 PF24814 345 - 471 9.3e-13 Prp19 WD40 domain
WD40 PF00400 347 - 382 1.2e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 348 - 426 5.8e-12 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 349 - 409 6.4e-08 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 349 - 451 3.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 352 - 408 2.6e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 355 - 563 1.2e-21 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 367 - 471 1.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 370 - 473 2.5e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 388 - 424 1.2e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 398 - 568 5e-10 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1395
Acc36I ACCTGC 1 cut(s) 2048
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 2 cut(s) 120, 1772
AccBSI CCGCTC 1 cut(s) 1735
AccI GTMKAC 1 cut(s) 1665
AccII CGCG 1 cut(s) 45
AciI CCGC 8 cut(s) 43, 703, 944, 1328, 1387, 1735, 1860, 2055
AclI AACGTT 1 cut(s) 1809
AclWI GGATC 7 cut(s) 306, 881, 1089, 1493, 1918, 1974, 2081
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 6 cut(s) 23, 374, 388, 776, 1275, 1542
AcuI CTGAAG 4 cut(s) 543, 572, 1605, 1877
AfaI GTAC 5 cut(s) 797, 1311, 1442, 1988, 2106
AfiI CCNNNNNNNGG 7 cut(s) 120, 702, 1095, 1772, 2034, 2035, 2061
AgsI TTSAA 9 cut(s) 342, 354, 467, 709, 762, 1208, 1213, 1248, 1780
AjnI CCWGG 2 cut(s) 744, 1088
AluBI AGCT 5 cut(s) 9, 521, 722, 1358, 1936
AluI AGCT 5 cut(s) 9, 521, 722, 1358, 1936
Alw21I GWGCWC 1 cut(s) 2018
AlwI GGATC 7 cut(s) 306, 881, 1089, 1493, 1918, 1974, 2081
AlwNI CAGNNNCTG 2 cut(s) 65, 2108
Ama87I CYCGRG 2 cut(s) 163, 1925
AoxI GGCC 6 cut(s) 427, 543, 747, 768, 1788, 1922
ApeKI GCWGC 1 cut(s) 1592
ApoI RAATTY 6 cut(s) 23, 374, 388, 776, 1275, 1542
ArsI GACNNNNNNTTYG 2 cut(s) 1500, 1532
AspLEI GCGC 3 cut(s) 480, 1526, 1952
AspS9I GGNCC 5 cut(s) 428, 543, 588, 748, 1923
AsuC2I CCSGG 4 cut(s) 164, 165, 431, 697
AsuHPI GGTGA 5 cut(s) 824, 898, 1082, 1732, 1848
AvaI CYCGRG 2 cut(s) 163, 1925
AvaII GGWCC 1 cut(s) 588
BalI TGGCCA 1 cut(s) 770
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 2 cut(s) 342, 1110
Bbv12I GWGCWC 1 cut(s) 2018
BbvI GCAGC 1 cut(s) 1604
BccI CCATC 9 cut(s) 199, 377, 782, 812, 898, 1247, 1340, 1619, 2060
BcgI CGANNNNNNTGC 4 cut(s) 35, 69, 566, 600
BciT130I CCWGG 2 cut(s) 746, 1090
BclI TGATCA 1 cut(s) 1684
BcnI CCSGG 4 cut(s) 164, 165, 431, 697
BfaI CTAG 4 cut(s) 255, 800, 1503, 1803
BfmI CTRYAG 1 cut(s) 1678
BfoI RGCGCY 1 cut(s) 1953
BfuAI ACCTGC 1 cut(s) 2048
BglII AGATCT 1 cut(s) 1604
BisI GCNGC 2 cut(s) 1387, 1593
BlsI GCNGC 2 cut(s) 1388, 1594
Bme1390I CCNGG 6 cut(s) 164, 165, 431, 697, 746, 1090
Bme18I GGWCC 1 cut(s) 588
BmeT110I CYCGRG 2 cut(s) 163, 1925
BmgT120I GGNCC 5 cut(s) 428, 543, 588, 748, 1923
BmiI GGNNCC 3 cut(s) 126, 544, 1458
BmrFI CCNGG 6 cut(s) 164, 165, 431, 697, 746, 1090
BmsI GCATC 5 cut(s) 28, 58, 65, 1145, 1738
BpiI GAAGAC 2 cut(s) 342, 1110
BpuMI CCSGG 4 cut(s) 164, 165, 431, 697
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 1 cut(s) 316
BsaJI CCNNGG 4 cut(s) 163, 164, 1766, 1926
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 1347, 1377
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 702, 1095, 1772, 2034, 2035, 2061
Bse1I ACTGG 1 cut(s) 2102
Bse3DI GCAATG 1 cut(s) 1043
Bse8I GATNNNNATC 1 cut(s) 316
BseBI CCWGG 2 cut(s) 746, 1090
BseDI CCNNGG 4 cut(s) 163, 164, 1766, 1926
BseGI GGATG 5 cut(s) 210, 890, 1160, 1228, 2071
BseJI GATNNNNATC 1 cut(s) 316
BseLI CCNNNNNNNGG 7 cut(s) 120, 702, 1095, 1772, 2034, 2035, 2061
BseMI GCAATG 1 cut(s) 1043
BseMII CTCAG 4 cut(s) 465, 732, 1592, 1865
BseNI ACTGG 1 cut(s) 2102
BseRI GAGGAG 2 cut(s) 236, 1943
BseXI GCAGC 1 cut(s) 1604
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 6 cut(s) 429, 545, 749, 770, 1790, 1924
BshNI GGYRCC 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 2018
BsiHKCI CYCGRG 2 cut(s) 163, 1925
BsiSI CCGG 4 cut(s) 128, 164, 431, 696
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 7 cut(s) 120, 702, 1095, 1772, 2034, 2035, 2061
BsmFI GGGAC 1 cut(s) 747
BsmI GAATGC 1 cut(s) 1594
BsnI GGCC 6 cut(s) 429, 545, 749, 770, 1790, 1924
BsoBI CYCGRG 2 cut(s) 163, 1925
Bsp1286I GDGCHC 1 cut(s) 2018
Bsp1407I TGTACA 1 cut(s) 1440
Bsp143I GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
Bsp19I CCATGG 1 cut(s) 1766
BspACI CCGC 8 cut(s) 43, 703, 944, 1328, 1387, 1735, 1860, 2055
BspANI GGCC 6 cut(s) 429, 545, 749, 770, 1790, 1924
BspCNI CTCAG 4 cut(s) 466, 731, 1593, 1866
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 126, 544, 1458
BspMAI CTGCAG 1 cut(s) 1682
BspMI ACCTGC 1 cut(s) 2048
BspPI GGATC 7 cut(s) 306, 881, 1089, 1493, 1918, 1974, 2081
BspT107I GGYRCC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 1735
BsrDI GCAATG 1 cut(s) 1043
BsrGI TGTACA 1 cut(s) 1440
BsrI ACTGG 1 cut(s) 2102
BssECI CCNNGG 4 cut(s) 163, 164, 1766, 1926
BssMI GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
BssNAI GTATAC 1 cut(s) 1666
BssT1I CCWWGG 1 cut(s) 1766
Bst1107I GTATAC 1 cut(s) 1666
Bst2UI CCWGG 2 cut(s) 746, 1090
Bst4CI ACNGT 5 cut(s) 795, 910, 1113, 1135, 1445
Bst6I CTCTTC 4 cut(s) 12, 462, 519, 548
BstAPI GCANNNNNTGC 1 cut(s) 1164
BstAUI TGTACA 1 cut(s) 1440
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 2 cut(s) 43, 1864
BstDEI CTNAG 5 cut(s) 474, 718, 1601, 1792, 1874
BstDSI CCRYGG 1 cut(s) 1766
BstEII GGTNACC 1 cut(s) 2097
BstENI CCTNNNNNAGG 1 cut(s) 1093
BstF5I GGATG 5 cut(s) 210, 890, 1160, 1228, 2071
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 1953
BstHHI GCGC 3 cut(s) 480, 1526, 1952
BstKTI GATC 9 cut(s) 314, 889, 1084, 1501, 1607, 1687, 1913, 1982, 2076
BstMBI GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
BstMWI GCNNNNNNNGC 5 cut(s) 835, 1164, 1263, 1386, 1757
BstNI CCWGG 2 cut(s) 746, 1090
BstNSI RCATGY 3 cut(s) 600, 1002, 1866
BstPI GGTNACC 1 cut(s) 2097
BstSCI CCNGG 6 cut(s) 162, 163, 429, 695, 744, 1088
BstSFI CTRYAG 1 cut(s) 1678
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 1604
BstV2I GAAGAC 2 cut(s) 342, 1110
BstX2I RGATCY 7 cut(s) 311, 886, 1081, 1498, 1604, 1910, 1979
BstXI CCANNNNNNTGG 1 cut(s) 2093
BstYI RGATCY 7 cut(s) 311, 886, 1081, 1498, 1604, 1910, 1979
BstZ17I GTATAC 1 cut(s) 1666
BsuRI GGCC 6 cut(s) 429, 545, 749, 770, 1790, 1924
BtgI CCRYGG 1 cut(s) 1766
BtgZI GCGATG 1 cut(s) 1320
BtsCI GGATG 5 cut(s) 210, 890, 1160, 1228, 2071
BtsI GCAGTG 1 cut(s) 1687
BtsIMutI CAGTG 6 cut(s) 66, 451, 1158, 1246, 1687, 1743
BveI ACCTGC 1 cut(s) 2048
Cac8I GCNNGC 2 cut(s) 43, 1864
CaiI CAGNNNCTG 2 cut(s) 65, 2108
CfoI GCGC 3 cut(s) 480, 1526, 1952
Cfr13I GGNCC 5 cut(s) 428, 543, 588, 748, 1923
Cfr9I CCCGGG 1 cut(s) 163
CseI GACGC 2 cut(s) 170, 1433
Csp6I GTAC 5 cut(s) 796, 1310, 1441, 1987, 2105
CviQI GTAC 5 cut(s) 796, 1310, 1441, 1987, 2105
DdeI CTNAG 5 cut(s) 474, 718, 1601, 1792, 1874
DpnI GATC 9 cut(s) 313, 888, 1083, 1500, 1606, 1686, 1912, 1981, 2075
DpnII GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
DraI TTTAAA 1 cut(s) 1999
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 4 cut(s) 12, 462, 519, 548
EarI CTCTTC 4 cut(s) 12, 462, 519, 548
Eco130I CCWWGG 1 cut(s) 1766
Eco147I AGGCCT 1 cut(s) 1790
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 1 cut(s) 588
Eco57I CTGAAG 4 cut(s) 543, 572, 1605, 1877
Eco88I CYCGRG 2 cut(s) 163, 1925
Eco91I GGTNACC 1 cut(s) 2097
EcoNI CCTNNNNNAGG 1 cut(s) 1093
EcoO109I RGGNCCY 1 cut(s) 543
EcoO65I GGTNACC 1 cut(s) 2097
EcoRI GAATTC 3 cut(s) 23, 1275, 1542
EcoRII CCWGG 2 cut(s) 744, 1088
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 1766
EcoT22I ATGCAT 1 cut(s) 2035
ErhI CCWWGG 1 cut(s) 1766
FalI AAGNNNNNCTT 4 cut(s) 334, 366, 530, 562
FaqI GGGAC 1 cut(s) 747
FauI CCCGC 2 cut(s) 50, 1867
FauNDI CATATG 2 cut(s) 1382, 1670
FbaI TGATCA 1 cut(s) 1684
FblI GTMKAC 1 cut(s) 1665
Fnu4HI GCNGC 2 cut(s) 1387, 1593
FokI GGATG 5 cut(s) 217, 877, 1167, 1235, 2078
Fsp4HI GCNGC 2 cut(s) 1387, 1593
FspBI CTAG 4 cut(s) 255, 800, 1503, 1803
GlaI GCGC 3 cut(s) 479, 1525, 1951
GluI GCNGC 2 cut(s) 1387, 1593
HaeII RGCGCY 1 cut(s) 1953
HaeIII GGCC 6 cut(s) 429, 545, 749, 770, 1790, 1924
HapII CCGG 4 cut(s) 128, 164, 431, 696
HgaI GACGC 2 cut(s) 170, 1433
HhaI GCGC 3 cut(s) 480, 1526, 1952
Hin6I GCGC 3 cut(s) 478, 1524, 1950
HinP1I GCGC 3 cut(s) 478, 1524, 1950
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 1934
HinfI GANTC 8 cut(s) 470, 831, 850, 913, 992, 1529, 1655, 1883
HpaII CCGG 4 cut(s) 128, 164, 431, 696
HphI GGTGA 5 cut(s) 824, 898, 1082, 1732, 1848
Hpy166II GTNNAC 4 cut(s) 1036, 1126, 1666, 1740
Hpy188III TCNNGA 7 cut(s) 77, 197, 364, 455, 785, 878, 1688
Hpy8I GTNNAC 4 cut(s) 1036, 1126, 1666, 1740
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 8 cut(s) 169, 433, 443, 676, 703, 1447, 1901, 2064
HpyCH4III ACNGT 5 cut(s) 795, 910, 1113, 1135, 1445
HpyCH4IV ACGT 2 cut(s) 970, 1809
HpyF10VI GCNNNNNNNGC 5 cut(s) 835, 1164, 1263, 1386, 1757
HpyF3I CTNAG 5 cut(s) 474, 718, 1601, 1792, 1874
HpySE526I ACGT 2 cut(s) 970, 1809
HspAI GCGC 3 cut(s) 478, 1524, 1950
Ksp22I TGATCA 1 cut(s) 1684
Kzo9I GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
LmnI GCTCC 4 cut(s) 6, 178, 1355, 1462
Lsp1109I GCAGC 1 cut(s) 1604
LweI GCATC 5 cut(s) 28, 58, 65, 1145, 1738
MaeI CTAG 4 cut(s) 255, 800, 1503, 1803
MaeII ACGT 2 cut(s) 970, 1809
MaeIII GTNAC 4 cut(s) 1195, 1516, 2050, 2097
MalI GATC 9 cut(s) 313, 888, 1083, 1500, 1606, 1686, 1912, 1981, 2075
MbiI CCGCTC 1 cut(s) 1735
MboI GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 7 cut(s) 311, 886, 1081, 1498, 1604, 1910, 1979
MhlI GDGCHC 1 cut(s) 2018
MlsI TGGCCA 1 cut(s) 770
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 2 cut(s) 479, 840
MmeI TCCRAC 3 cut(s) 75, 1546, 1964
Mox20I TGGCCA 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 2035
MscI TGGCCA 1 cut(s) 770
MseI TTAA 3 cut(s) 189, 561, 1998
MslI CAYNNNNRTG 4 cut(s) 89, 1068, 1861, 2091
Msp20I TGGCCA 1 cut(s) 770
MspI CCGG 4 cut(s) 128, 164, 431, 696
MspR9I CCNGG 6 cut(s) 164, 165, 431, 697, 746, 1090
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 1594
MvaI CCWGG 2 cut(s) 746, 1090
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 5 cut(s) 835, 1164, 1263, 1386, 1757
NciI CCSGG 4 cut(s) 164, 165, 431, 697
NcoI CCATGG 1 cut(s) 1766
NdeI CATATG 2 cut(s) 1382, 1670
NdeII GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
NlaIV GGNNCC 3 cut(s) 126, 544, 1458
NmuCI GTSAC 3 cut(s) 1195, 1516, 2050
NsiI ATGCAT 1 cut(s) 2035
NspI RCATGY 3 cut(s) 600, 1002, 1866
PaeI GCATGC 1 cut(s) 1866
PceI AGGCCT 1 cut(s) 1790
PcsI WCGNNNNNNNCGW 1 cut(s) 1815
PctI GAATGC 1 cut(s) 1594
PfeI GAWTC 6 cut(s) 850, 913, 992, 1529, 1655, 1883
PflFI GACNNNGTC 1 cut(s) 1960
PflMI CCANNNNNTGG 2 cut(s) 120, 1772
PfoI TCCNGGA 1 cut(s) 695
PkrI GCNGC 2 cut(s) 1388, 1594
PleI GAGTC 2 cut(s) 478, 839
PpsI GAGTC 2 cut(s) 478, 839
Ppu21I YACGTR 1 cut(s) 971
PsiI TTATAA 1 cut(s) 1395
Psp1406I AACGTT 1 cut(s) 1809
Psp6I CCWGG 2 cut(s) 744, 1088
PspEI GGTNACC 1 cut(s) 2097
PspGI CCWGG 2 cut(s) 744, 1088
PspN4I GGNNCC 3 cut(s) 126, 544, 1458
PspPI GGNCC 5 cut(s) 428, 543, 588, 748, 1923
PstI CTGCAG 1 cut(s) 1682
PstNI CAGNNNCTG 2 cut(s) 65, 2108
PsuI RGATCY 7 cut(s) 311, 886, 1081, 1498, 1604, 1910, 1979
PsyI GACNNNGTC 1 cut(s) 1960
RsaI GTAC 5 cut(s) 797, 1311, 1442, 1988, 2106
RsaNI GTAC 5 cut(s) 796, 1310, 1441, 1987, 2105
RseI CAYNNNNRTG 4 cut(s) 89, 1068, 1861, 2091
SaqAI TTAA 3 cut(s) 189, 561, 1998
SatI GCNGC 2 cut(s) 1387, 1593
Sau3AI GATC 9 cut(s) 311, 886, 1081, 1498, 1604, 1684, 1910, 1979, 2073
Sau96I GGNCC 5 cut(s) 428, 543, 588, 748, 1923
SchI GAGTC 2 cut(s) 479, 840
ScrFI CCNGG 6 cut(s) 164, 165, 431, 697, 746, 1090
SduI GDGCHC 1 cut(s) 2018
SfaNI GCATC 5 cut(s) 28, 58, 65, 1145, 1738
SfcI CTRYAG 1 cut(s) 1678
SinI GGWCC 1 cut(s) 588
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 4 cut(s) 89, 1068, 1861, 2091
SphI GCATGC 1 cut(s) 1866
SseBI AGGCCT 1 cut(s) 1790
SsiI CCGC 8 cut(s) 43, 703, 944, 1328, 1387, 1735, 1860, 2055
SspMI CTAG 4 cut(s) 255, 800, 1503, 1803
StuI AGGCCT 1 cut(s) 1790
StyD4I CCNGG 6 cut(s) 162, 163, 429, 695, 744, 1088
StyI CCWWGG 1 cut(s) 1766
TaaI ACNGT 5 cut(s) 795, 910, 1113, 1135, 1445
TaiI ACGT 2 cut(s) 973, 1812
TaqI TCGA 5 cut(s) 454, 1370, 1482, 2021, 2113
TaqII GACCGA 2 cut(s) 336, 576
TatI WGTACW 2 cut(s) 1440, 1986
TauI GCSGC 1 cut(s) 1389
TfiI GAWTC 6 cut(s) 850, 913, 992, 1529, 1655, 1883
Tru1I TTAA 3 cut(s) 189, 561, 1998
Tru9I TTAA 3 cut(s) 189, 561, 1998
TscAI CASTG 6 cut(s) 66, 451, 1165, 1246, 1687, 1750
TseFI GTSAC 3 cut(s) 1195, 1516, 2050
TseI GCWGC 1 cut(s) 1592
Tsp45I GTSAC 3 cut(s) 1195, 1516, 2050
TspDTI ATGAA 7 cut(s) 387, 435, 788, 879, 1046, 1538, 1995
TspGWI ACGGA 2 cut(s) 1600, 1744
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 6 cut(s) 66, 451, 1165, 1246, 1687, 1750
Tth111I GACNNNGTC 1 cut(s) 1960
Van91I CCANNNNNTGG 2 cut(s) 120, 1772
VpaK11BI GGWCC 1 cut(s) 588
XagI CCTNNNNNAGG 1 cut(s) 1093
XapI RAATTY 6 cut(s) 23, 374, 388, 776, 1275, 1542
XceI RCATGY 3 cut(s) 600, 1002, 1866
XmaI CCCGGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 1665
XspI CTAG 4 cut(s) 255, 800, 1503, 1803
Zsp2I ATGCAT 1 cut(s) 2035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.