MD08G1004600.v1.1

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017388
Physical Location & Seq
Forward (+)
423160 .. 426815
3656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1004600.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGCTAATCAACTTGGTTAATTTGCTATCAAACATGAAGACCAGAAGCAAAATTCCACAGCAGAACTCAACTCTTCTGTCACTTATTATTGTTAGCGTTTTAGTTAGCTTCCCTGTTACAAGATCAGACGACGGTAGTAATGGGGAGTATTTGATTGGAGTGGGCAGCTTTGACATGACTGGCCCGGCTGCCGGGGTCAACATGATGGGTTATGCCAGCATGGACCAGAATACCGCCGGTGTACACTTCCGGCTAAGGGCAAGAACCTTCATCGTGGCCGAGAGTTCCCAAGGTCCAAGGTTCGCCTTTGTTAATCTTGATGCTGGAATGGCTTCACAACTGGTTAACATTAAAGTGCTCGACAAACTCAAATCAAGGTTTGGAAACTTGTACACTGAAGACAATGTGGCGATTAGCGGGATTCACACGCACGCAGGACCAGGGGGTTATTTGCAGTATCTAGTTTACACTGTAACATCTTTGGGCTTTGTCCAACAATCCTTCGATGCCATCGTCAATGCAATTGTGCAGAGCATTGTTCTGGCTCATCACAACCTCAAGCCTGGTTCCATTTTCATCAACCAAGGGGATGTGGTAAACGCGGGAATAAACAGGAGTCCGAGTGCTTATCTGCTAAACCCGGCAGAGGAGAGAGCACGGTACCCGAAAGATGTGGATACTCTGATGACTCTATTGAAGTTTGTGGATGGCGGAAGCGGGAAGAGCATCGGAGCATTCAGCTGGTTTGCCACCCACGGAACCTCCATGAGCAAAGACAACAAGCTCATCAGTGGCGACAACAAAGGTGCCGCTGCCAGATTCTTCGAGGACTGGTTCACTACAACTAATACTAGTACTGGTGCCACGATATCATCTCCACCAGATAGTAGCGTGGCAGCAGCGCTAATAAAGAAGGCGGAAAATATCAAAGCAACGGGAGGGAAGCCCTGCGGCAAAACAACCAGTCGCAAGTTTAAGGTGAGGAAGAACGATGGCTCACTATTTGTGGGAGCCTTTTGCCAATCCAACGTGGGAGATGTGACTCCAAATGTCCTTGGAGCGTTTTGCACCGATTCTGGAAAACCTTGCGACTTCACTCACTCCTCCTGTCATGGGAACAACCAACTCTGCCTCGGACGTGGACCTGGGTACCCGGATGAAATACAAAGCACGAAAATTATAGGGGAGAGGCAATTTCTAAGGGCGGCTGATCTATTCACGTCGGCTACAGAGCAGCTGACCGGGAAAATAGACTTTCGCCACGTGTACCTAGATTTCACGAATATTGAGGTTGATTTGGAAGGAAACAAGAAAGTGAAAACATGCCCCGCGGGGCTTGGTCCGGGATTTGCCGCTGGGACTACAGATGGACCGGGTGTCTTTGGCTTCCAACAGGGTGATCAAAAGATCAATTCGACATGGAGAAGACTGAGGGATTCGCTAAAAAAACCCAGCCAATATCAAGTGAACTGCCAACAGCCCAAGGCAGTTCTGCTCAGCACTGGGGAAATGTTTGTACCTTATGCATGGGCGCCTGCAATCGTTCCTATTCAAATGCTCAGGTTGGGGAAGCTGATCATACTTTCTGTCCCAGGAGAATTCACTACAATGGCCGGTCGGCGGCTAAGGGAAGCGGTCAAGGAGACCCTTATAAGTAACAGCAACGGTGAATTTGATGAGGATACTCGCATTGTAATAGCAGGGCTTACTAATACTTACTCGCAATATATTGCAACTTTCGAGGAATACGCACAACAACGATATGAGGCTGCTTCCACTCTCTATGGTCCTCACACGTTATCAGCGTACATTCAAGAATTCAATAAATTAGCCAAGGCGATGGCAAAGGGAGACAAAGACCTTCCTAAAGGTCCCTTACCACCAGATCTTTCCTCTGTACAAATCAGAGTAGGTGATCCTACTGGAGACTCACCTCCCCCAGGTACAAAATTTGGAGATATTAAGGAGGATGTCAGCTTACCAAAAAGTGGATCATTCCAAAAGGGAGATAGACCAACTGCCACATTTTGGAGCGCTAACCCAAGATATGACCTATTGACGGAAGGGACATATGCAGTGGTAGAGATGCTTCAAGGAAAACACTGGGTTCCTGTTTACGACGACGATGATTTCTCCCTGTTTTTCAAGTGGAATGTAGATACCAGTAGCTTATATGGCACAGCAACCATAGAATGGGAAATACCGACGGATACAAATTCAGGGGTGTACAGGCTAAGGCATTTTGGTTCAGCAAAGGAAACAAAGGACTCACCCAACACCTACTTCACCGGCGCATCTAGTGGATTTGCAGTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

773

Amino Acids

83.78

Weight (kDa)

7.94

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 50 - 605 2.2e-217 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 607 - 771 2.4e-43 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1652
Acc65I GGTACC 2 cut(s) 660, 1149
AccB1I GGYRCC 5 cut(s) 660, 806, 860, 1149, 1531
AccB7I CCANNNNNTGG 3 cut(s) 1988, 2028, 2193
AccII CGCG 2 cut(s) 602, 1331
AclWI GGATC 2 cut(s) 1910, 1999
AcoI YGGCCR 2 cut(s) 276, 1611
AcsI RAATTY 6 cut(s) 51, 1598, 1670, 1817, 1949, 2215
AcuI CTGAAG 1 cut(s) 417
AcvI CACGTG 1 cut(s) 1264
AcyI GRCGYC 1 cut(s) 1532
AfeI AGCGCT 2 cut(s) 903, 2035
AflIII ACRYGT 2 cut(s) 1263, 1794
AgsI TTSAA 6 cut(s) 697, 1553, 1814, 1822, 2093, 2146
AhdI GACNNNNNGTC 1 cut(s) 1376
AhlI ACTAGT 1 cut(s) 851
AjiI CACGTC 2 cut(s) 1139, 1221
AjnI CCWGG 5 cut(s) 439, 562, 1144, 1591, 1939
AjuI GAANNNNNNNTTGG 2 cut(s) 2267, 2299
AluBI AGCT 8 cut(s) 108, 168, 741, 784, 1237, 1573, 1977, 2169
AluI AGCT 8 cut(s) 108, 168, 741, 784, 1237, 1573, 1977, 2169
Alw21I GWGCWC 2 cut(s) 360, 658
Alw26I GTCTC 3 cut(s) 1637, 1845, 1920
AlwI GGATC 2 cut(s) 1910, 1999
Aor51HI AGCGCT 2 cut(s) 903, 2035
AoxI GGCC 3 cut(s) 181, 276, 1611
ApeKI GCWGC 7 cut(s) 165, 188, 812, 896, 899, 1234, 1769
ApoI RAATTY 6 cut(s) 51, 1598, 1670, 1817, 1949, 2215
Asp700I GAANNNNTTC 1 cut(s) 331
Asp718I GGTACC 2 cut(s) 660, 1149
AspLEI GCGC 4 cut(s) 904, 1534, 2036, 2294
AspS9I GGNCC 9 cut(s) 182, 223, 293, 437, 1142, 1340, 1370, 1787, 1871
AsuC2I CCSGG 7 cut(s) 185, 193, 641, 1154, 1243, 1344, 1374
AsuHPI GGTGA 7 cut(s) 991, 1409, 1679, 1923, 1925, 2262, 2278
AvaII GGWCC 8 cut(s) 223, 293, 437, 1142, 1340, 1370, 1787, 1871
BaeI ACNNNNGTAYC 2 cut(s) 1133, 1166
BanI GGYRCC 5 cut(s) 660, 806, 860, 1149, 1531
BarI GAAGNNNNNNTAC 2 cut(s) 2073, 2105
BbrPI CACGTG 1 cut(s) 1264
BbsI GAAGAC 3 cut(s) 44, 405, 1432
Bbv12I GWGCWC 2 cut(s) 360, 658
BbvI GCAGC 7 cut(s) 175, 177, 799, 908, 911, 1246, 1756
BccI CCATC 6 cut(s) 199, 518, 701, 986, 1361, 1834
BciT130I CCWGG 5 cut(s) 441, 564, 1146, 1593, 1941
BciVI GTATCC 3 cut(s) 670, 1675, 2203
BclI TGATCA 2 cut(s) 1399, 1575
BcnI CCSGG 7 cut(s) 185, 193, 641, 1154, 1243, 1344, 1374
BcoDI GTCTC 3 cut(s) 1637, 1845, 1920
BcuI ACTAGT 1 cut(s) 851
BfaI CTAG 4 cut(s) 461, 852, 1271, 2298
BfmI CTRYAG 2 cut(s) 1227, 1362
BfoI RGCGCY 3 cut(s) 905, 1535, 2037
BfuI GTATCC 3 cut(s) 670, 1675, 2203
BglII AGATCT 1 cut(s) 1885
BlpI GCTNAGC 1 cut(s) 1496
BmcAI AGTACT 1 cut(s) 856
Bme18I GGWCC 8 cut(s) 223, 293, 437, 1142, 1340, 1370, 1787, 1871
BmeRI GACNNNNNGTC 1 cut(s) 1376
BmgBI CACGTC 2 cut(s) 1139, 1221
BmgT120I GGNCC 9 cut(s) 182, 223, 293, 437, 1142, 1340, 1370, 1787, 1871
BmrI ACTGGG 2 cut(s) 1512, 2113
BmsI GCATC 5 cut(s) 310, 496, 735, 2076, 2303
BmuI ACTGGG 2 cut(s) 1512, 2113
BpiI GAAGAC 3 cut(s) 44, 405, 1432
BpmI CTGGAG 1 cut(s) 1944
Bpu10I CCTNAGC 4 cut(s) 254, 1559, 1625, 2234
Bpu1102I GCTNAGC 1 cut(s) 1496
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 7 cut(s) 185, 193, 641, 1154, 1243, 1344, 1374
BsaAI YACGTR 1 cut(s) 1264
BsaHI GRCGYC 1 cut(s) 1532
BsaI GGTCTC 1 cut(s) 1637
BsaXI ACNNNNNCTCC 4 cut(s) 607, 637, 746, 776
Bse118I RCCGGY 3 cut(s) 236, 1613, 2288
Bse1I ACTGG 9 cut(s) 184, 345, 836, 862, 963, 1507, 1927, 2108, 2163
BseBI CCWGG 5 cut(s) 441, 564, 1146, 1593, 1941
BseGI GGATG 4 cut(s) 595, 712, 1162, 1975
BseMII CTCAG 3 cut(s) 1421, 1510, 1573
BseNI ACTGG 9 cut(s) 184, 345, 836, 862, 963, 1507, 1927, 2108, 2163
BseRI GAGGAG 2 cut(s) 662, 1093
BseXI GCAGC 7 cut(s) 175, 177, 799, 908, 911, 1246, 1756
BseYI CCCAGC 2 cut(s) 1355, 1451
BsgI GTGCAG 1 cut(s) 548
Bsh1236I CGCG 2 cut(s) 602, 1331
Bsh1285I CGRYCG 1 cut(s) 1618
BshFI GGCC 3 cut(s) 183, 278, 1613
BshNI GGYRCC 5 cut(s) 660, 806, 860, 1149, 1531
BsiEI CGRYCG 1 cut(s) 1618
BsiHKAI GWGCWC 2 cut(s) 360, 658
BslFI GGGAC 4 cut(s) 1372, 1574, 1857, 2080
BsmAI GTCTC 3 cut(s) 1637, 1845, 1920
BsmFI GGGAC 4 cut(s) 1372, 1574, 1857, 2080
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 3 cut(s) 183, 278, 1613
Bso31I GGTCTC 1 cut(s) 1637
Bsp1286I GDGCHC 2 cut(s) 360, 658
Bsp1407I TGTACA 4 cut(s) 241, 390, 1897, 2226
Bsp143I GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
Bsp1720I GCTNAGC 1 cut(s) 1496
BspANI GGCC 3 cut(s) 183, 278, 1613
BspCNI CTCAG 3 cut(s) 1422, 1509, 1572
BspFNI CGCG 2 cut(s) 602, 1331
BspPI GGATC 2 cut(s) 1910, 1999
BspQI GCTCTTC 1 cut(s) 716
BspT107I GGYRCC 5 cut(s) 660, 806, 860, 1149, 1531
BspTNI GGTCTC 1 cut(s) 1637
BsrFI RCCGGY 3 cut(s) 236, 1613, 2288
BsrGI TGTACA 4 cut(s) 241, 390, 1897, 2226
BsrI ACTGG 9 cut(s) 184, 345, 836, 862, 963, 1507, 1927, 2108, 2163
BssAI RCCGGY 3 cut(s) 236, 1613, 2288
BssMI GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
BssNI GRCGYC 1 cut(s) 1532
BssT1I CCWWGG 6 cut(s) 289, 296, 583, 1054, 1482, 1833
Bst2UI CCWGG 5 cut(s) 441, 564, 1146, 1593, 1941
Bst4CI ACNGT 4 cut(s) 134, 472, 660, 1667
Bst6I CTCTTC 2 cut(s) 78, 716
BstACI GRCGYC 1 cut(s) 1532
BstAUI TGTACA 4 cut(s) 241, 390, 1897, 2226
BstBAI YACGTR 1 cut(s) 1264
BstC8I GCNNGC 3 cut(s) 217, 432, 1536
BstDEI CTNAG 7 cut(s) 254, 1199, 1430, 1496, 1559, 1625, 2234
BstDSI CCRYGG 2 cut(s) 754, 1329
BstENI CCTNNNNNAGG 1 cut(s) 1938
BstF5I GGATG 4 cut(s) 595, 712, 1162, 1975
BstFNI CGCG 2 cut(s) 602, 1331
BstH2I RGCGCY 3 cut(s) 905, 1535, 2037
BstHHI GCGC 4 cut(s) 904, 1534, 2036, 2294
BstKTI GATC 8 cut(s) 125, 1213, 1402, 1410, 1578, 1888, 1918, 1994
BstMAI GTCTC 3 cut(s) 1637, 1845, 1920
BstMBI GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
BstMCI CGRYCG 1 cut(s) 1618
BstMWI GCNNNNNNNGC 3 cut(s) 329, 723, 2175
BstNI CCWGG 5 cut(s) 441, 564, 1146, 1593, 1941
BstNSI RCATGY 1 cut(s) 1326
BstSFI CTRYAG 2 cut(s) 1227, 1362
BstUI CGCG 2 cut(s) 602, 1331
BstV1I GCAGC 7 cut(s) 175, 177, 799, 908, 911, 1246, 1756
BstV2I GAAGAC 3 cut(s) 44, 405, 1432
BstX2I RGATCY 1 cut(s) 1885
BstXI CCANNNNNNTGG 1 cut(s) 1840
BstYI RGATCY 1 cut(s) 1885
BsuI GTATCC 3 cut(s) 670, 1675, 2203
BsuRI GGCC 3 cut(s) 183, 278, 1613
BtgI CCRYGG 2 cut(s) 754, 1329
BtgZI GCGATG 1 cut(s) 1853
BtrI CACGTC 2 cut(s) 1139, 1221
BtsCI GGATG 4 cut(s) 595, 712, 1162, 1975
BtsI GCAGTG 2 cut(s) 2082, 2316
BtsIMutI CAGTG 7 cut(s) 393, 468, 796, 1500, 2082, 2101, 2316
Cac8I GCNNGC 3 cut(s) 217, 432, 1536
CfoI GCGC 4 cut(s) 904, 1534, 2036, 2294
Cfr10I RCCGGY 3 cut(s) 236, 1613, 2288
Cfr13I GGNCC 9 cut(s) 182, 223, 293, 437, 1142, 1340, 1370, 1787, 1871
Cfr42I CCGCGG 1 cut(s) 1332
CviAII CATG 9 cut(s) 34, 175, 202, 220, 766, 1112, 1323, 1419, 1527
DdeI CTNAG 7 cut(s) 254, 1199, 1430, 1496, 1559, 1625, 2234
DinI GGCGCC 1 cut(s) 1533
DpnI GATC 8 cut(s) 124, 1212, 1401, 1409, 1577, 1887, 1917, 1993
DpnII GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
DriI GACNNNNNGTC 1 cut(s) 1376
EaeI YGGCCR 2 cut(s) 276, 1611
Eam1104I CTCTTC 2 cut(s) 78, 716
Eam1105I GACNNNNNGTC 1 cut(s) 1376
EarI CTCTTC 2 cut(s) 78, 716
EciI GGCGGA 2 cut(s) 726, 932
Eco130I CCWWGG 6 cut(s) 289, 296, 583, 1054, 1482, 1833
Eco31I GGTCTC 1 cut(s) 1637
Eco32I GATATC 1 cut(s) 870
Eco47I GGWCC 8 cut(s) 223, 293, 437, 1142, 1340, 1370, 1787, 1871
Eco47III AGCGCT 2 cut(s) 903, 2035
Eco57I CTGAAG 1 cut(s) 417
Eco72I CACGTG 1 cut(s) 1264
EcoNI CCTNNNNNAGG 1 cut(s) 1938
EcoO109I RGGNCCY 1 cut(s) 1871
EcoRI GAATTC 2 cut(s) 1598, 1817
EcoRII CCWGG 5 cut(s) 439, 562, 1144, 1591, 1939
EcoRV GATATC 1 cut(s) 870
EcoT14I CCWWGG 6 cut(s) 289, 296, 583, 1054, 1482, 1833
EcoT22I ATGCAT 1 cut(s) 1528
EgeI GGCGCC 1 cut(s) 1533
EheI GGCGCC 1 cut(s) 1533
ErhI CCWWGG 6 cut(s) 289, 296, 583, 1054, 1482, 1833
FaeI CATG 9 cut(s) 37, 178, 205, 223, 769, 1115, 1326, 1422, 1530
FaqI GGGAC 4 cut(s) 1372, 1574, 1857, 2080
FatI CATG 9 cut(s) 33, 174, 201, 219, 765, 1111, 1322, 1418, 1526
FauI CCCGC 5 cut(s) 410, 595, 710, 1324, 1336
FauNDI CATATG 1 cut(s) 2071
FbaI TGATCA 2 cut(s) 1399, 1575
FokI GGATG 4 cut(s) 602, 719, 1169, 1982
FspBI CTAG 4 cut(s) 461, 852, 1271, 2298
GlaI GCGC 4 cut(s) 903, 1533, 2035, 2293
GsaI CCCAGC 2 cut(s) 1359, 1455
GsuI CTGGAG 1 cut(s) 1944
HaeII RGCGCY 3 cut(s) 905, 1535, 2037
HaeIII GGCC 3 cut(s) 183, 278, 1613
HhaI GCGC 4 cut(s) 904, 1534, 2036, 2294
Hin1I GRCGYC 1 cut(s) 1532
Hin1II CATG 9 cut(s) 37, 178, 205, 223, 769, 1115, 1326, 1422, 1530
Hin6I GCGC 4 cut(s) 902, 1532, 2034, 2292
HinP1I GCGC 4 cut(s) 902, 1532, 2034, 2292
HincII GTYRAC 2 cut(s) 199, 346
HindII GTYRAC 2 cut(s) 199, 346
HinfI GANTC 9 cut(s) 421, 616, 688, 819, 1042, 1073, 1436, 1928, 2267
HpaI GTTAAC 1 cut(s) 346
HphI GGTGA 7 cut(s) 991, 1409, 1679, 1923, 1925, 2262, 2278
Hpy188I TCNGA 6 cut(s) 127, 621, 684, 731, 1136, 1907
Hpy188III TCNNGA 4 cut(s) 317, 1077, 1279, 1814
Hpy99I CGWCG 5 cut(s) 134, 1225, 2123, 2126, 2209
HpyAV CCTTC 6 cut(s) 277, 511, 907, 1295, 1871, 2057
HpyCH4III ACNGT 4 cut(s) 134, 472, 660, 1667
HpyCH4IV ACGT 5 cut(s) 1029, 1138, 1220, 1263, 1796
HpyCH4V TGCA 9 cut(s) 454, 521, 529, 1068, 1526, 1538, 1733, 2075, 2309
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 723, 2175
HpyF3I CTNAG 7 cut(s) 254, 1199, 1430, 1496, 1559, 1625, 2234
HpySE526I ACGT 5 cut(s) 1029, 1138, 1220, 1263, 1796
Hsp92I GRCGYC 1 cut(s) 1532
Hsp92II CATG 9 cut(s) 37, 178, 205, 223, 769, 1115, 1326, 1422, 1530
HspAI GCGC 4 cut(s) 902, 1532, 2034, 2292
KasI GGCGCC 1 cut(s) 1531
KpnI GGTACC 2 cut(s) 664, 1153
Ksp22I TGATCA 2 cut(s) 1399, 1575
KspAI GTTAAC 1 cut(s) 346
KspI CCGCGG 1 cut(s) 1332
Kzo9I GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
LguI GCTCTTC 1 cut(s) 716
LmnI GCTCC 4 cut(s) 731, 1010, 1058, 2031
Lsp1109I GCAGC 7 cut(s) 175, 177, 799, 908, 911, 1246, 1756
LweI GCATC 5 cut(s) 310, 496, 735, 2076, 2303
MaeI CTAG 4 cut(s) 461, 852, 1271, 2298
MaeII ACGT 5 cut(s) 1029, 1138, 1220, 1263, 1796
MaeIII GTNAC 5 cut(s) 78, 115, 472, 1039, 1655
MalI GATC 8 cut(s) 124, 1212, 1401, 1409, 1577, 1887, 1917, 1993
MboI GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
MboII GAAGA 7 cut(s) 49, 65, 410, 733, 814, 997, 1437
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 1885
MhlI GDGCHC 2 cut(s) 360, 658
Mly113I GGCGCC 1 cut(s) 1532
MlyI GAGTC 5 cut(s) 625, 682, 1036, 1922, 2261
MmeI TCCRAC 3 cut(s) 517, 1050, 1414
Mph1103I ATGCAT 1 cut(s) 1528
MroXI GAANNNNTTC 1 cut(s) 331
MseI TTAA 7 cut(s) 18, 312, 345, 351, 975, 1962, 2317
MslI CAYNNNNRTG 2 cut(s) 353, 1607
MspA1I CMGCKG 5 cut(s) 741, 812, 1237, 1331, 1355
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 5 cut(s) 441, 564, 1146, 1593, 1941
MvnI CGCG 2 cut(s) 602, 1331
MwoI GCNNNNNNNGC 3 cut(s) 329, 723, 2175
NarI GGCGCC 1 cut(s) 1532
NciI CCSGG 7 cut(s) 185, 193, 641, 1154, 1243, 1344, 1374
NdeI CATATG 1 cut(s) 2071
NdeII GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
NlaIII CATG 9 cut(s) 37, 178, 205, 223, 769, 1115, 1326, 1422, 1530
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 78, 1039
NsiI ATGCAT 1 cut(s) 1528
NspI RCATGY 1 cut(s) 1326
PciSI GCTCTTC 1 cut(s) 716
PcsI WCGNNNNNNNCGW 2 cut(s) 510, 1802
PctI GAATGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 421, 819, 1073, 1436
PflMI CCANNNNNTGG 3 cut(s) 1988, 2028, 2193
PfoI TCCNGGA 1 cut(s) 1342
PleI GAGTC 5 cut(s) 624, 682, 1036, 1922, 2261
PluTI GGCGCC 1 cut(s) 1535
PmaCI CACGTG 1 cut(s) 1264
PmlI CACGTG 1 cut(s) 1264
PpsI GAGTC 5 cut(s) 624, 682, 1036, 1922, 2261
Ppu21I YACGTR 1 cut(s) 1264
PpuMI RGGWCCY 1 cut(s) 1871
PsiI TTATAA 1 cut(s) 1652
Psp5II RGGWCCY 1 cut(s) 1871
Psp6I CCWGG 5 cut(s) 439, 562, 1144, 1591, 1939
PspCI CACGTG 1 cut(s) 1264
PspFI CCCAGC 2 cut(s) 1355, 1451
PspGI CCWGG 5 cut(s) 439, 562, 1144, 1591, 1939
PspPI GGNCC 9 cut(s) 182, 223, 293, 437, 1142, 1340, 1370, 1787, 1871
PspPPI RGGWCCY 1 cut(s) 1871
PsuI RGATCY 1 cut(s) 1885
PvuII CAGCTG 2 cut(s) 741, 1237
RseI CAYNNNNRTG 2 cut(s) 353, 1607
SacII CCGCGG 1 cut(s) 1332
SapI GCTCTTC 1 cut(s) 716
SaqAI TTAA 7 cut(s) 18, 312, 345, 351, 975, 1962, 2317
Sau3AI GATC 8 cut(s) 122, 1210, 1399, 1407, 1575, 1885, 1915, 1991
Sau96I GGNCC 9 cut(s) 182, 223, 293, 437, 1142, 1340, 1370, 1787, 1871
ScaI AGTACT 1 cut(s) 856
SchI GAGTC 5 cut(s) 625, 682, 1036, 1922, 2261
SduI GDGCHC 2 cut(s) 360, 658
SfaNI GCATC 5 cut(s) 310, 496, 735, 2076, 2303
SfcI CTRYAG 2 cut(s) 1227, 1362
SfoI GGCGCC 1 cut(s) 1533
Sfr303I CCGCGG 1 cut(s) 1332
SgrAI CRCCGGYG 2 cut(s) 236, 2288
SgrBI CCGCGG 1 cut(s) 1332
SinI GGWCC 8 cut(s) 223, 293, 437, 1142, 1340, 1370, 1787, 1871
SmiMI CAYNNNNRTG 2 cut(s) 353, 1607
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SpeI ACTAGT 1 cut(s) 851
SspDI GGCGCC 1 cut(s) 1531
SspI AATATT 1 cut(s) 1285
SspMI CTAG 4 cut(s) 461, 852, 1271, 2298
StyI CCWWGG 6 cut(s) 289, 296, 583, 1054, 1482, 1833
TaaI ACNGT 4 cut(s) 134, 472, 660, 1667
TaiI ACGT 5 cut(s) 1032, 1141, 1223, 1266, 1799
TaqI TCGA 5 cut(s) 360, 504, 825, 1415, 1740
TatI WGTACW 5 cut(s) 241, 390, 854, 1897, 2226
TauI GCSGC 5 cut(s) 812, 954, 1208, 1355, 1624
TfiI GAWTC 4 cut(s) 421, 819, 1073, 1436
Tru1I TTAA 7 cut(s) 18, 312, 345, 351, 975, 1962, 2317
Tru9I TTAA 7 cut(s) 18, 312, 345, 351, 975, 1962, 2317
TscAI CASTG 7 cut(s) 400, 475, 796, 1507, 2082, 2108, 2316
TseFI GTSAC 2 cut(s) 78, 1039
TseI GCWGC 7 cut(s) 165, 188, 812, 896, 899, 1234, 1769
Tsp45I GTSAC 2 cut(s) 78, 1039
TspDTI ATGAA 4 cut(s) 50, 259, 565, 1173
TspGWI ACGGA 3 cut(s) 771, 2075, 2222
TspRI CASTG 7 cut(s) 400, 475, 796, 1507, 2082, 2108, 2316
Van91I CCANNNNNTGG 3 cut(s) 1988, 2028, 2193
VpaK11BI GGWCC 8 cut(s) 223, 293, 437, 1142, 1340, 1370, 1787, 1871
XagI CCTNNNNNAGG 1 cut(s) 1938
XapI RAATTY 6 cut(s) 51, 1598, 1670, 1817, 1949, 2215
XceI RCATGY 1 cut(s) 1326
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 4 cut(s) 461, 852, 1271, 2298
ZrmI AGTACT 1 cut(s) 856
Zsp2I ATGCAT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.