Rw6G038200

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017388
Physical Location & Seq
Reverse (-)
62409142 .. 62412649
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038200.1

Sequence Viewer

Length: 2325 bp
ATGAAGATCAAAAGCAAATTTCCTCACACAAGGACACTCTTATCACTTACTGTTGTTGCTATATGTGTTATAAAATCAGTGAGTTGTAGTTCTACTACTGATGATAATGGCAAGTATTTGATCGGAGTTGGAAGCTATGACATGACCGGACCGGCAGCCGGAGTGGTCATGATGGGTTACGCCAACATGGGCCAAACCACTGCGGGTGTACATTTCCGGTTAAGGGCAAGGACTTTCATCGTGGCCGAGAGCTCCCAAGGTCCAAGGTTTGCCTTTGTTAATCTTGATGCAGGGATGGCTTCACAACTCGTCAATATTAAAGTGCTCGAGAAACTGAAATCAAGGTTTGGCGATTTGTATACCGAAGAAAATGTGGCGATTAGTGGCATTCACACCCATGCAGGACCTGGAGGTTATTTGCAGTATTTTCTTTACTCTATATCAACACTAGGATTCATCCAAGAATCATTCGACGTCATTGTCAACGCAATTGTGCAAAGCATTGTTCAGGCTCATCATAACCTCAAGCCTGGTTCTATTTATATCAACCAAGGGGATGTGGTAAACGCCGGAATAAACAGGAGTCCAAGCGCTTATTTGCTAAACCCAGCAGAGGAGAGAGCACAGTATCCGACCAACGTTGATACCCTAATGACTCTTTTGAAGTTTGTGGATGGTGCCAGCCAACAGAGCATTGGAGCATTCAGCTGGTTTGCCACTCACGGAACCTCCATGAGCAAAGAGAATAAGCTCATCAGTGGAGACAACAAAGGTGCAGCTGCACGGTTCTTCGAGGACCAGTTTGCTACAACTCCCATCAGAGCACCCTTCAACTCCTCTAGCAAAGCAGGTAAGGAGTTAAAGCTCGTCAGAATACTGGGCAACATTAATATTGGTGAGTTAGTAAAGAAGGCGCAGACAATCAAAGCAACAGGAGGGCAGCGCTGCAGTAAAACGAGCAGCCAAGACTGTAAGGTGAGGAAGAACGACGGGTCACTATTCGTGGGAGCTTTTTGCCAATCTAATGTTGGAGATGTGACCCCGAATGTGCTCGGAGCATTCTGCACTGATACCGGAAGACCTTGTGACTTCAATACATCCTCATGTAATGGGAACGACCTGCTTTGCGTCGGCCGGGGACCCGGGTATCCTGATGAAAGACTAAGCACCAAAATTATAGGGGAAAGACAGTTTAAGAAGGCAGCTGATCTATTTACATCGGCTTCAGAGAAATTAACGGGGGCTATTGATTACCGTCACGTGTACCTAGACTTTACGGATATTGAGGTTGAGCTAGAAGGAAACAAGAAGGTGAAAACATGTCCAGCAGCAGTTGGTGCTGGTTTTGCTGCTGGGACTACAGATGGACCTGGTGTCTCTGGGTTTCAACAAGGTGATACAGAGATCCCTGAAAAATATAGAAAACTTAGGGACGCACTGCAAAAACCTAGTGAATATCAAGTGGACTGCCAAGAACCCAAGAATGTTCTGCTTTCCACCGGTGAAATGTTTAAACCTTACGCATGGGCGCCTGCAATTGTCCCTATACAAATGCTCAGATTGGGGAAACTCATCATACTTTCTGTCCCAGGAGAATTCACCACAATGGCGGGGAGGCGGCTAAGGGACGCAGTAAAGGAGACATTGATAAGTAACAGCAAGGGTGAATTTGATGAAAACACCCACATAGTAATAGCAGGCCTTACAAATACTTATTCCCAATATATAGCAACTTTCGAAGAGTACACACAACAGAGATATGAGGCTGCTTCCACTCTCTATGGTCCTCACACATTATCAGCATACATCCAAGAATTTGAGAAACTAGCCAAAGCAATGGCCAAGGGAGAAAAGGTCATTTCTAAAGGTCCCTCGCCACCAGATCTTTCCTCTGTACAAGTTGAAGTCTTACTTGGACCTTTTGGAGATTCGCCTCCACCACATACAAAGTTTGGAGATATGAAACAAGATATCATTATACCAAAAAGTGGTTCATTCAGAAAGGGAGATTTGAATAGACCAAATGCCACATTTTGGAGTGCAAACCCAAACTATGACTTGTTGACAGAAGGCACATATGCTGTGGTAGAGATGCTTCAAGGGGATAACTGGGTTCCAGTTTACGATGATGATGATTTCTCCGTGGTTTTCAAGTGGAATAGAGATGACAAGGGATTATATAGTGCAGCAACCATAGAATGGGAAATACCAATTTATGCAAATTTGGGGGTGTACAGGCTCAGGCATTTTGGTTCATCAAGGAAAAAGAAGGACTCCCCCATCATCTACTTCACTGGTGCATCTAGTGGGTTTGCAGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

774

Amino Acids

84.44

Weight (kDa)

8.09

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 39 - 604 9.4e-214 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 606 - 773 2.4e-41 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 2 cut(s) 839, 1128
AccB1I GGYRCC 2 cut(s) 677, 1528
AccB7I CCANNNNNTGG 3 cut(s) 1988, 2034, 2199
AccI GTMKAC 1 cut(s) 359
AciI CCGC 3 cut(s) 203, 1610, 1618
AclI AACGTT 1 cut(s) 639
AclWI GGATC 1 cut(s) 1399
AcoI YGGCCR 3 cut(s) 243, 1132, 1839
AcsI RAATTY 5 cut(s) 17, 1595, 1667, 1814, 2221
AcuI CTGAAG 1 cut(s) 1209
AcvI CACGTG 1 cut(s) 1261
AcyI GRCGYC 2 cut(s) 474, 1529
AfaI GTAC 5 cut(s) 210, 1265, 1745, 1896, 2234
AfeI AGCGCT 2 cut(s) 592, 944
AfiI CCNNNNNNNGG 6 cut(s) 158, 223, 613, 1988, 2034, 2199
AflIII ACRYGT 2 cut(s) 1260, 1319
AgeI ACCGGT 1 cut(s) 1499
AgsI TTSAA 8 cut(s) 664, 832, 1093, 1388, 1904, 2014, 2099, 2152
AhdI GACNNNNNGTC 1 cut(s) 1373
AjnI CCWGG 4 cut(s) 406, 529, 1369, 1588
AjuI GAANNNNNNNTTGG 4 cut(s) 1896, 1928, 1975, 2007
AluBI AGCT 9 cut(s) 135, 252, 708, 751, 779, 865, 1010, 1205, 1294
AluI AGCT 9 cut(s) 135, 252, 708, 751, 779, 865, 1010, 1205, 1294
Alw21I GWGCWC 5 cut(s) 254, 327, 625, 826, 1053
Alw26I GTCTC 3 cut(s) 756, 1381, 1634
AlwI GGATC 1 cut(s) 1399
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 2 cut(s) 326, 1142
Aor51HI AGCGCT 2 cut(s) 592, 944
AoxI GGCC 5 cut(s) 190, 243, 1132, 1699, 1839
ApoI RAATTY 5 cut(s) 17, 1595, 1667, 1814, 2221
AseI ATTAAT 1 cut(s) 888
AsiGI ACCGGT 1 cut(s) 1499
AspLEI GCGC 4 cut(s) 593, 916, 945, 1531
AsuC2I CCSGG 3 cut(s) 1136, 1143, 1144
AsuHPI GGTGA 7 cut(s) 908, 988, 1324, 1406, 1514, 1591, 1676
AsuII TTCGAA 1 cut(s) 1737
AvaI CYCGRG 2 cut(s) 326, 1142
AvaII GGWCC 9 cut(s) 149, 260, 404, 796, 1139, 1367, 1784, 1868, 1916
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BalI TGGCCA 1 cut(s) 1841
BanI GGYRCC 2 cut(s) 677, 1528
BanII GRGCYC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 2079, 2111
BbrPI CACGTG 1 cut(s) 1261
BbsI GAAGAC 1 cut(s) 1084
Bbv12I GWGCWC 5 cut(s) 254, 327, 625, 826, 1053
BccI CCATC 6 cut(s) 166, 289, 668, 824, 1358, 2288
BciT130I CCWGG 4 cut(s) 408, 531, 1371, 1590
BciVI GTATCC 2 cut(s) 639, 1158
BcnI CCSGG 3 cut(s) 1136, 1143, 1144
BcoDI GTCTC 3 cut(s) 756, 1381, 1634
BfaI CTAG 7 cut(s) 449, 840, 1268, 1295, 1449, 1826, 2304
BfmI CTRYAG 2 cut(s) 946, 1359
BfoI RGCGCY 3 cut(s) 594, 946, 1532
BfuAI ACCTGC 2 cut(s) 839, 1128
BfuI GTATCC 2 cut(s) 639, 1158
BglII AGATCT 1 cut(s) 1882
Bme1390I CCNGG 7 cut(s) 408, 531, 1136, 1143, 1144, 1371, 1590
Bme18I GGWCC 9 cut(s) 149, 260, 404, 796, 1139, 1367, 1784, 1868, 1916
BmeRI GACNNNNNGTC 1 cut(s) 1373
BmeT110I CYCGRG 2 cut(s) 326, 1142
BmiI GGNNCC 7 cut(s) 679, 727, 1140, 1141, 1530, 1870, 2115
BmrFI CCNGG 7 cut(s) 408, 531, 1136, 1143, 1144, 1371, 1590
BmrI ACTGGG 2 cut(s) 887, 2119
BmsI GCATC 3 cut(s) 277, 2082, 2309
BmuI ACTGGG 2 cut(s) 887, 2119
BpiI GAAGAC 1 cut(s) 1084
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 1622, 2240
Bpu14I TTCGAA 1 cut(s) 1737
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 3 cut(s) 1136, 1143, 1144
BsaAI YACGTR 1 cut(s) 1261
BsaHI GRCGYC 2 cut(s) 474, 1529
BsaJI CCNNGG 8 cut(s) 256, 263, 550, 1135, 1142, 1588, 1842, 2142
BsaWI WCCGGW 4 cut(s) 146, 216, 1073, 1499
BsaXI ACNNNNNCTCC 4 cut(s) 713, 743, 1839, 1869
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 223, 613, 1988, 2034, 2199
Bse118I RCCGGY 2 cut(s) 151, 1499
Bse1I ACTGG 5 cut(s) 799, 882, 2114, 2117, 2299
Bse3DI GCAATG 1 cut(s) 1842
BseBI CCWGG 4 cut(s) 408, 531, 1371, 1590
BseDI CCNNGG 8 cut(s) 256, 263, 550, 1135, 1142, 1588, 1842, 2142
BseGI GGATG 6 cut(s) 300, 456, 562, 679, 1097, 1806
BseLI CCNNNNNNNGG 6 cut(s) 158, 223, 613, 1988, 2034, 2199
BseMI GCAATG 1 cut(s) 1842
BseMII CTCAG 2 cut(s) 1570, 2254
BseNI ACTGG 5 cut(s) 799, 882, 2114, 2117, 2299
BseRI GAGGAG 2 cut(s) 629, 826
BseX3I CGGCCG 1 cut(s) 1132
BseYI CCCAGC 2 cut(s) 607, 1352
BsgI GTGCAG 4 cut(s) 765, 795, 1048, 2205
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 5 cut(s) 192, 245, 1134, 1701, 1841
BshNI GGYRCC 2 cut(s) 677, 1528
BshTI ACCGGT 1 cut(s) 1499
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 5 cut(s) 254, 327, 625, 826, 1053
BsiHKCI CYCGRG 2 cut(s) 326, 1142
BsiSI CCGG 9 cut(s) 147, 152, 159, 217, 570, 1074, 1135, 1143, 1500
BslFI GGGAC 7 cut(s) 1152, 1369, 1445, 1526, 1571, 1640, 1854
BslI CCNNNNNNNGG 6 cut(s) 158, 223, 613, 1988, 2034, 2199
BsmAI GTCTC 3 cut(s) 756, 1381, 1634
BsmFI GGGAC 7 cut(s) 1152, 1369, 1445, 1526, 1571, 1640, 1854
BsmI GAATGC 3 cut(s) 387, 701, 1058
BsnI GGCC 5 cut(s) 192, 245, 1134, 1701, 1841
BsoBI CYCGRG 2 cut(s) 326, 1142
Bsp119I TTCGAA 1 cut(s) 1737
Bsp1286I GDGCHC 5 cut(s) 254, 327, 625, 826, 1053
Bsp1407I TGTACA 3 cut(s) 208, 1894, 2232
Bsp143I GATC 5 cut(s) 6, 120, 1207, 1404, 1882
BspACI CCGC 3 cut(s) 203, 1610, 1618
BspANI GGCC 5 cut(s) 192, 245, 1134, 1701, 1841
BspCNI CTCAG 2 cut(s) 1569, 2253
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 7 cut(s) 679, 727, 1140, 1141, 1530, 1870, 2115
BspMAI CTGCAG 1 cut(s) 950
BspMI ACCTGC 2 cut(s) 839, 1128
BspPI GGATC 1 cut(s) 1399
BspT104I TTCGAA 1 cut(s) 1737
BspT107I GGYRCC 2 cut(s) 677, 1528
BsrDI GCAATG 1 cut(s) 1842
BsrFI RCCGGY 2 cut(s) 151, 1499
BsrGI TGTACA 3 cut(s) 208, 1894, 2232
BsrI ACTGG 5 cut(s) 799, 882, 2114, 2117, 2299
BssAI RCCGGY 2 cut(s) 151, 1499
BssECI CCNNGG 8 cut(s) 256, 263, 550, 1135, 1142, 1588, 1842, 2142
BssMI GATC 5 cut(s) 6, 120, 1207, 1404, 1882
BssNAI GTATAC 1 cut(s) 360
BssNI GRCGYC 2 cut(s) 474, 1529
BssT1I CCWWGG 4 cut(s) 256, 263, 550, 1842
Bst1107I GTATAC 1 cut(s) 360
Bst2UI CCWGG 4 cut(s) 408, 531, 1371, 1590
Bst4CI ACNGT 6 cut(s) 52, 627, 786, 971, 1191, 1256
Bst6I CTCTTC 1 cut(s) 1734
BstACI GRCGYC 2 cut(s) 474, 1529
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstAUI TGTACA 3 cut(s) 208, 1894, 2232
BstBAI YACGTR 1 cut(s) 1261
BstBI TTCGAA 1 cut(s) 1737
BstC8I GCNNGC 3 cut(s) 682, 1533, 1699
BstDEI CTNAG 5 cut(s) 1163, 1427, 1556, 1622, 2240
BstDSI CCRYGG 1 cut(s) 2142
BstF5I GGATG 6 cut(s) 300, 456, 562, 679, 1097, 1806
BstH2I RGCGCY 3 cut(s) 594, 946, 1532
BstHHI GCGC 4 cut(s) 593, 916, 945, 1531
BstKTI GATC 5 cut(s) 9, 123, 1210, 1407, 1885
BstMAI GTCTC 3 cut(s) 756, 1381, 1634
BstMBI GATC 5 cut(s) 6, 120, 1207, 1404, 1882
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 4 cut(s) 296, 690, 1337, 1346
BstNI CCWGG 4 cut(s) 408, 531, 1371, 1590
BstNSI RCATGY 1 cut(s) 1323
BstSCI CCNGG 7 cut(s) 406, 529, 1134, 1141, 1142, 1369, 1588
BstSFI CTRYAG 2 cut(s) 946, 1359
BstV2I GAAGAC 1 cut(s) 1084
BstX2I RGATCY 2 cut(s) 1404, 1882
BstXI CCANNNNNNTGG 1 cut(s) 1837
BstYI RGATCY 2 cut(s) 1404, 1882
BstZ17I GTATAC 1 cut(s) 360
BstZI CGGCCG 1 cut(s) 1132
BsuI GTATCC 2 cut(s) 639, 1158
BsuRI GGCC 5 cut(s) 192, 245, 1134, 1701, 1841
BtgI CCRYGG 1 cut(s) 2142
BtsCI GGATG 6 cut(s) 300, 456, 562, 679, 1097, 1806
BtsI GCAGTG 3 cut(s) 198, 1436, 2322
BtsIMutI CAGTG 7 cut(s) 84, 198, 763, 1065, 1436, 2292, 2322
BveI ACCTGC 2 cut(s) 839, 1128
Cac8I GCNNGC 3 cut(s) 682, 1533, 1699
CaiI CAGNNNCTG 1 cut(s) 407
CciI TCATGA 1 cut(s) 168
CfoI GCGC 4 cut(s) 593, 916, 945, 1531
Cfr10I RCCGGY 2 cut(s) 151, 1499
Cfr9I CCCGGG 1 cut(s) 1142
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 3 cut(s) 1117, 1442, 1637
CsiI ACCWGGT 1 cut(s) 1369
Csp6I GTAC 5 cut(s) 209, 1264, 1744, 1895, 2233
CspAI ACCGGT 1 cut(s) 1499
CspI CGGWCCG 1 cut(s) 149
CviAII CATG 8 cut(s) 142, 169, 187, 398, 733, 1104, 1320, 1524
CviQI GTAC 5 cut(s) 209, 1264, 1744, 1895, 2233
DdeI CTNAG 5 cut(s) 1163, 1427, 1556, 1622, 2240
DinI GGCGCC 1 cut(s) 1530
DpnI GATC 5 cut(s) 8, 122, 1209, 1406, 1884
DpnII GATC 5 cut(s) 6, 120, 1207, 1404, 1882
DraI TTTAAA 1 cut(s) 1513
DrdI GACNNNNNNGTC 1 cut(s) 479
DriI GACNNNNNGTC 1 cut(s) 1373
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 3 cut(s) 243, 1132, 1839
EagI CGGCCG 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 1734
Eam1105I GACNNNNNGTC 1 cut(s) 1373
EarI CTCTTC 1 cut(s) 1734
Ecl136II GAGCTC 1 cut(s) 252
EclXI CGGCCG 1 cut(s) 1132
Eco130I CCWWGG 4 cut(s) 256, 263, 550, 1842
Eco147I AGGCCT 1 cut(s) 1701
Eco24I GRGCYC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 1972
Eco47I GGWCC 9 cut(s) 149, 260, 404, 796, 1139, 1367, 1784, 1868, 1916
Eco47III AGCGCT 2 cut(s) 592, 944
Eco52I CGGCCG 1 cut(s) 1132
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 1209
Eco72I CACGTG 1 cut(s) 1261
Eco88I CYCGRG 2 cut(s) 326, 1142
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 3 cut(s) 404, 1139, 1868
EcoRI GAATTC 1 cut(s) 1595
EcoRII CCWGG 4 cut(s) 406, 529, 1369, 1588
EcoRV GATATC 1 cut(s) 1972
EcoT14I CCWWGG 4 cut(s) 256, 263, 550, 1842
EcoT38I GRGCYC 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 1530
EheI GGCGCC 1 cut(s) 1530
ErhI CCWWGG 4 cut(s) 256, 263, 550, 1842
FaeI CATG 8 cut(s) 145, 172, 190, 401, 736, 1107, 1323, 1527
FalI AAGNNNNNCTT 2 cut(s) 1896, 1928
FaqI GGGAC 7 cut(s) 1152, 1369, 1445, 1526, 1571, 1640, 1854
FatI CATG 8 cut(s) 141, 168, 186, 397, 732, 1103, 1319, 1523
FauI CCCGC 2 cut(s) 196, 1603
FauNDI CATATG 1 cut(s) 2077
FblI GTMKAC 1 cut(s) 359
FokI GGATG 6 cut(s) 307, 443, 569, 686, 1084, 1793
FriOI GRGCYC 1 cut(s) 254
FspBI CTAG 7 cut(s) 449, 840, 1268, 1295, 1449, 1826, 2304
GlaI GCGC 4 cut(s) 592, 915, 944, 1530
GsaI CCCAGC 2 cut(s) 611, 1356
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 3 cut(s) 594, 946, 1532
HaeIII GGCC 5 cut(s) 192, 245, 1134, 1701, 1841
HapII CCGG 9 cut(s) 147, 152, 159, 217, 570, 1074, 1135, 1143, 1500
HgaI GACGC 3 cut(s) 1117, 1442, 1637
HhaI GCGC 4 cut(s) 593, 916, 945, 1531
Hin1I GRCGYC 2 cut(s) 474, 1529
Hin1II CATG 8 cut(s) 145, 172, 190, 401, 736, 1107, 1323, 1527
Hin6I GCGC 4 cut(s) 591, 914, 943, 1529
HinP1I GCGC 4 cut(s) 591, 914, 943, 1529
HincII GTYRAC 2 cut(s) 484, 2064
HindII GTYRAC 2 cut(s) 484, 2064
HinfI GANTC 6 cut(s) 453, 464, 583, 655, 1928, 2273
HpaII CCGG 9 cut(s) 147, 152, 159, 217, 570, 1074, 1135, 1143, 1500
HphI GGTGA 7 cut(s) 908, 988, 1324, 1406, 1514, 1591, 1676
Hpy188I TCNGA 8 cut(s) 125, 633, 821, 872, 1055, 1228, 1559, 2000
Hpy188III TCNNGA 4 cut(s) 169, 284, 328, 1151
Hpy99I CGWCG 3 cut(s) 476, 992, 1133
HpyAV CCTTC 7 cut(s) 838, 904, 1192, 1292, 1303, 2063, 2263
HpyCH4III ACNGT 6 cut(s) 52, 627, 786, 971, 1191, 1256
HpyCH4IV ACGT 3 cut(s) 474, 639, 1260
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 690, 1337, 1346
HpyF3I CTNAG 5 cut(s) 1163, 1427, 1556, 1622, 2240
HpySE526I ACGT 3 cut(s) 474, 639, 1260
Hsp92I GRCGYC 2 cut(s) 474, 1529
Hsp92II CATG 8 cut(s) 145, 172, 190, 401, 736, 1107, 1323, 1527
HspAI GCGC 4 cut(s) 591, 914, 943, 1529
KasI GGCGCC 1 cut(s) 1528
KflI GGGWCCC 1 cut(s) 1139
Kzo9I GATC 5 cut(s) 6, 120, 1207, 1404, 1882
LmnI GCTCC 4 cut(s) 257, 698, 1007, 1055
LweI GCATC 3 cut(s) 277, 2082, 2309
MabI ACCWGGT 1 cut(s) 1369
MaeI CTAG 7 cut(s) 449, 840, 1268, 1295, 1449, 1826, 2304
MaeII ACGT 3 cut(s) 474, 639, 1260
MaeIII GTNAC 6 cut(s) 176, 993, 1036, 1085, 1256, 1652
MalI GATC 5 cut(s) 8, 122, 1209, 1406, 1884
MboI GATC 5 cut(s) 6, 120, 1207, 1404, 1882
MboII GAAGA 6 cut(s) 16, 377, 781, 994, 1089, 1751
MfeI CAATTG 2 cut(s) 489, 1536
MflI RGATCY 2 cut(s) 1404, 1882
MhlI GDGCHC 5 cut(s) 254, 327, 625, 826, 1053
MlsI TGGCCA 1 cut(s) 1841
MluNI TGGCCA 1 cut(s) 1841
Mly113I GGCGCC 1 cut(s) 1529
MlyI GAGTC 3 cut(s) 592, 649, 2267
MmeI TCCRAC 3 cut(s) 109, 656, 1009
Mox20I TGGCCA 1 cut(s) 1841
MscI TGGCCA 1 cut(s) 1841
MseI TTAA 8 cut(s) 221, 279, 318, 860, 888, 1194, 1235, 1512
MslI CAYNNNNRTG 3 cut(s) 396, 1102, 1604
Msp20I TGGCCA 1 cut(s) 1841
MspA1I CMGCKG 3 cut(s) 708, 779, 1205
MspI CCGG 9 cut(s) 147, 152, 159, 217, 570, 1074, 1135, 1143, 1500
MspR9I CCNGG 7 cut(s) 408, 531, 1136, 1143, 1144, 1371, 1590
MssI GTTTAAAC 1 cut(s) 1513
MunI CAATTG 2 cut(s) 489, 1536
Mva1269I GAATGC 3 cut(s) 387, 701, 1058
MvaI CCWGG 4 cut(s) 408, 531, 1371, 1590
MwoI GCNNNNNNNGC 4 cut(s) 296, 690, 1337, 1346
NarI GGCGCC 1 cut(s) 1529
NciI CCSGG 3 cut(s) 1136, 1143, 1144
NdeI CATATG 1 cut(s) 2077
NdeII GATC 5 cut(s) 6, 120, 1207, 1404, 1882
NlaIII CATG 8 cut(s) 145, 172, 190, 401, 736, 1107, 1323, 1527
NlaIV GGNNCC 7 cut(s) 679, 727, 1140, 1141, 1530, 1870, 2115
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 4 cut(s) 993, 1036, 1085, 1256
NspI RCATGY 1 cut(s) 1323
NspV TTCGAA 1 cut(s) 1737
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 168
PceI AGGCCT 1 cut(s) 1701
PciI ACATGT 1 cut(s) 1319
PctI GAATGC 3 cut(s) 387, 701, 1058
PfeI GAWTC 3 cut(s) 453, 464, 1928
PflMI CCANNNNNTGG 3 cut(s) 1988, 2034, 2199
PinAI ACCGGT 1 cut(s) 1499
PleI GAGTC 3 cut(s) 591, 649, 2267
PluTI GGCGCC 1 cut(s) 1532
PmaCI CACGTG 1 cut(s) 1261
PmeI GTTTAAAC 1 cut(s) 1513
PmlI CACGTG 1 cut(s) 1261
PpsI GAGTC 3 cut(s) 591, 649, 2267
Ppu21I YACGTR 1 cut(s) 1261
PpuMI RGGWCCY 3 cut(s) 404, 1139, 1868
PscI ACATGT 1 cut(s) 1319
PshBI ATTAAT 1 cut(s) 888
PsiI TTATAA 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 639
Psp5II RGGWCCY 3 cut(s) 404, 1139, 1868
Psp6I CCWGG 4 cut(s) 406, 529, 1369, 1588
PspCI CACGTG 1 cut(s) 1261
PspFI CCCAGC 2 cut(s) 607, 1352
PspGI CCWGG 4 cut(s) 406, 529, 1369, 1588
PspN4I GGNNCC 7 cut(s) 679, 727, 1140, 1141, 1530, 1870, 2115
PspPPI RGGWCCY 3 cut(s) 404, 1139, 1868
PstI CTGCAG 1 cut(s) 950
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 2 cut(s) 1404, 1882
PvuII CAGCTG 3 cut(s) 708, 779, 1205
RsaI GTAC 5 cut(s) 210, 1265, 1745, 1896, 2234
RsaNI GTAC 5 cut(s) 209, 1264, 1744, 1895, 2233
RseI CAYNNNNRTG 3 cut(s) 396, 1102, 1604
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 8 cut(s) 221, 279, 318, 860, 888, 1194, 1235, 1512
Sau3AI GATC 5 cut(s) 6, 120, 1207, 1404, 1882
SchI GAGTC 3 cut(s) 592, 649, 2267
ScrFI CCNGG 7 cut(s) 408, 531, 1136, 1143, 1144, 1371, 1590
SduI GDGCHC 5 cut(s) 254, 327, 625, 826, 1053
SexAI ACCWGGT 1 cut(s) 1369
SfaNI GCATC 3 cut(s) 277, 2082, 2309
SfcI CTRYAG 2 cut(s) 946, 1359
SfoI GGCGCC 1 cut(s) 1530
Sfr274I CTCGAG 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 1737
SgrAI CRCCGGYG 1 cut(s) 1499
SinI GGWCC 9 cut(s) 149, 260, 404, 796, 1139, 1367, 1784, 1868, 1916
SlaI CTCGAG 1 cut(s) 326
SmaI CCCGGG 1 cut(s) 1144
SmiMI CAYNNNNRTG 3 cut(s) 396, 1102, 1604
SmlI CTYRAG 2 cut(s) 326, 524
SmoI CTYRAG 2 cut(s) 326, 524
SseBI AGGCCT 1 cut(s) 1701
SsiI CCGC 3 cut(s) 203, 1610, 1618
SspDI GGCGCC 1 cut(s) 1528
SspI AATATT 2 cut(s) 316, 892
SspMI CTAG 7 cut(s) 449, 840, 1268, 1295, 1449, 1826, 2304
SstI GAGCTC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 1701
StyD4I CCNGG 7 cut(s) 406, 529, 1134, 1141, 1142, 1369, 1588
StyI CCWWGG 4 cut(s) 256, 263, 550, 1842
TaaI ACNGT 6 cut(s) 52, 627, 786, 971, 1191, 1256
TaiI ACGT 3 cut(s) 477, 642, 1263
TaqI TCGA 4 cut(s) 327, 471, 792, 1737
TatI WGTACW 4 cut(s) 208, 1743, 1894, 2232
TauI GCSGC 1 cut(s) 1621
TfiI GAWTC 3 cut(s) 453, 464, 1928
Tru1I TTAA 8 cut(s) 221, 279, 318, 860, 888, 1194, 1235, 1512
Tru9I TTAA 8 cut(s) 221, 279, 318, 860, 888, 1194, 1235, 1512
TscAI CASTG 7 cut(s) 84, 205, 763, 1072, 1443, 2299, 2322
TseFI GTSAC 4 cut(s) 993, 1036, 1085, 1256
Tsp45I GTSAC 4 cut(s) 993, 1036, 1085, 1256
TspDTI ATGAA 8 cut(s) 17, 226, 445, 1170, 1689, 1976, 1983, 2244
TspGWI ACGGA 3 cut(s) 738, 1292, 2131
TspMI CCCGGG 1 cut(s) 1142
TspRI CASTG 7 cut(s) 84, 205, 763, 1072, 1443, 2299, 2322
Van91I CCANNNNNTGG 3 cut(s) 1988, 2034, 2199
VpaK11BI GGWCC 9 cut(s) 149, 260, 404, 796, 1139, 1367, 1784, 1868, 1916
VspI ATTAAT 1 cut(s) 888
XapI RAATTY 5 cut(s) 17, 1595, 1667, 1814, 2221
XceI RCATGY 1 cut(s) 1323
XcmI CCANNNNNNNNNTGG 3 cut(s) 404, 692, 1025
XhoI CTCGAG 1 cut(s) 326
XmaI CCCGGG 1 cut(s) 1142
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 7 cut(s) 449, 840, 1268, 1295, 1449, 1826, 2304
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.