Rh6BG461900

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017388
Physical Location & Seq
Forward (+)
67202348 .. 67205863
3516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG461900.1

Sequence Viewer

Length: 2286 bp
ATGAAGATCAAAAGCAAATTTCCTCACACAAGGACACTCTTATCACTTACTGTTGTTGCTATATGTGTTATAAAATCAGTGAGTTGTACTTCTACTACTGATGATATTGGCGAGTTTTTGATCGGAGTTGGAAGCTATGACATGACCGGACCGGCAGCCGGAGTGGTCATGATGGGTTACGCCAACATGGGCCAAACCACTGCGGGTGTACATTTCCGGCTAAGGGCAAGGACTTTCATCGTGGCCGAGAGCTCCCAAGGTCCAAGGTTTGCCTTTGTTAATCTTGATGCAGGGATGGCTTCACAACTCGTTAATATTAAAGTGCTCGAGAAACTCAAATCAAGGTTTGGCGATTTGTATACCGAAGAAAATGTGGCGATTAGTGGCATTCACACCCATGCAGGACCTGGAGGTTATTTGCAGTATTTTCTTTACTCTATATCAACATTAGGATTCATCCAAGAATCATTCGATGTCATTGTCAACGCAATTGTGCAAAGCATTGTTCAGGCTCATCATAACCTCAAGCCTGGTTCTATTTTCATCAACCAAGGGGATGTGGTAAACGCCGGAATAAACAGGAGTCCAAGCGCTTATTTGCTAAACCCAGCAGAGGAGAGAGCACAGTATCCGACCAACGTTGATACCCTAATGACTCTTTTGAAGTTTGTGGATGGTGCCAGCCAACAGAGCATTGGAGCATTCAGCTGGTTTGCCACTCACGGAACCTCCATGAGCAAAGAGAACAAGCTCATCAGTGGAGACAACAAAGGTGCAGCTGCACGGTTCTTCGAGGACCAGTTTGCTACAACTCCCATCAGAGCACCCTTCAACTCCTCTAGCAAACCAGATATTGGTGAGTTAGTAAAGAAGGCGCAGACAATCAAAGCAACAGGAGGGCAGCGCTGCAGTAAAACGAGCAGCCAAGACTGCAAGGTGAGGAAGAACGACGGGTCACTATTCGTGGGAGCTTTTTGCCAATCTAATGTTGGAGATGTGACCCCGAATGTGCTCGGAGCATTCTGCACTGATACCGGAAGACCTTGTGACTTCAATACATCCTCATGTAATGGGAACGACCTGCTTTGCCTCGGCCAGGGACCTGGGTATCCTGATGAAATACTAAGCACCAAAATTATAGGGGAAAGACAGTTTAAGAAGGCAGCTGATCTATTTACATCGGCTTCAGAGAAATTAACCGGGGCTATTGATTACCGTCACGTGTACCTAGACTTTACGGATATTGAGGTTGAGCTAGAAGGAAACAAGAAGGTGAAAACATGTCCAGCAGCAGTTGGTGCTGGTTTTGCTGCTGGGACTACAGATGGACCTGGTGTCTCTGGGTTTCAACAAGGTGATACAGAGATCCCTGAAAAATATAGAAAACTTAGGGACGCACTGCAAAAACCTAGCGAATATCAAGTGGACTGCCAAGAACCCAAGAATGTTCTGCTTTCCACCGGTGAAATGTTTAAACCTTACGCATGGGCGCCTGCAATTGTCCCTATACAAATGCTCAGATTGGGAAAACTCATCATACTTTCTGTCCCAGGAGAATTCACCACAATGGCGGGGAGGCGGTTAAGGGACGCAGTAAAGGAGACATTGATAAGTAACAGCAAGGGTGAATTTGATGAAAACACCCACATAGTAATAGCAGGCCTTACAAACACTTATTCCCAATATATAGCAACTTTCGAAGAGTACACACAACAGAGATATGAGGCTGCTTCCACTCTCTATGGTCCTCACACATTATCAGCATACATCCAAGAATTTGAGAAATTAGCCAAAGCAATGGCCAAGGGAGAAAAGGTCATTTCTAAAGGTCCCTCGCCACCAGATCTTTCCTCTGTACAAGTCGAAGTCTTACTTGGACCTTTTGGAGATTCGCCTCCACCACATACAAAGTTTGGAGATATGAAACAAGATATCATTATACCAAAAAGTGGATCATTCAGAAAGGGAGATTTGAATAGACCAAATGCCACATTTTGGAGTGCAAACCCAAACTATGACTTGTTGACAGAAGGCACATATGCTGTGGTAGAGATGCTTCAAGGGGATAACTGGGTTCCAGTTTACGATGATGATGATTTCTCCGTGGTTTTCAAGTGGAATAGAGATGACAAGGGATTATATAGTGCAGCAACCATAGAATGGGAAATACCAATTTATGCAAATTTGGGGGTGTACAGGCTCAGGCATTTTGGTTCATCAAGGAAAAAGAAGGACTCCCCCATCACTTACTTCACTGGTGCATCTAGTGGGTTTGCAGTGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

761

Amino Acids

82.94

Weight (kDa)

6.36

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 39 - 591 1.3e-213 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 593 - 760 5.2e-42 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 1089
AccB1I GGYRCC 2 cut(s) 677, 1489
AccB7I CCANNNNNTGG 4 cut(s) 854, 1949, 1995, 2160
AccI GTMKAC 1 cut(s) 359
AciI CCGC 3 cut(s) 203, 1571, 1579
AclI AACGTT 1 cut(s) 639
AclWI GGATC 2 cut(s) 1360, 1960
AcoI YGGCCR 3 cut(s) 243, 1093, 1800
AcsI RAATTY 5 cut(s) 17, 1556, 1628, 1775, 2182
AcuI CTGAAG 1 cut(s) 1170
AcvI CACGTG 1 cut(s) 1222
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 6 cut(s) 88, 210, 1226, 1706, 1857, 2195
AfeI AGCGCT 2 cut(s) 592, 905
AfiI CCNNNNNNNGG 8 cut(s) 158, 223, 613, 854, 1096, 1949, 1995, 2160
AflIII ACRYGT 2 cut(s) 1221, 1280
AgeI ACCGGT 1 cut(s) 1460
AgsI TTSAA 7 cut(s) 664, 832, 1054, 1349, 1975, 2060, 2113
AhdI GACNNNNNGTC 1 cut(s) 1334
AjnI CCWGG 6 cut(s) 406, 529, 1095, 1102, 1330, 1549
AjuI GAANNNNNNNTTGG 2 cut(s) 1857, 1889
AluBI AGCT 8 cut(s) 135, 252, 708, 751, 779, 971, 1166, 1255
AluI AGCT 8 cut(s) 135, 252, 708, 751, 779, 971, 1166, 1255
Alw21I GWGCWC 5 cut(s) 254, 327, 625, 826, 1014
Alw26I GTCTC 3 cut(s) 756, 1342, 1595
AlwI GGATC 2 cut(s) 1360, 1960
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 326
Aor51HI AGCGCT 2 cut(s) 592, 905
AoxI GGCC 5 cut(s) 190, 243, 1093, 1660, 1800
ApoI RAATTY 5 cut(s) 17, 1556, 1628, 1775, 2182
AsiGI ACCGGT 1 cut(s) 1460
AspLEI GCGC 4 cut(s) 593, 877, 906, 1492
AsuC2I CCSGG 1 cut(s) 1201
AsuHPI GGTGA 7 cut(s) 869, 949, 1285, 1367, 1475, 1552, 1637
AsuII TTCGAA 1 cut(s) 1698
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 9 cut(s) 149, 260, 404, 796, 1100, 1328, 1745, 1829, 1877
BaeI ACNNNNGTAYC 2 cut(s) 1091, 1124
BalI TGGCCA 1 cut(s) 1802
BanI GGYRCC 2 cut(s) 677, 1489
BanII GRGCYC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 2040, 2072
BbrPI CACGTG 1 cut(s) 1222
BbsI GAAGAC 1 cut(s) 1045
Bbv12I GWGCWC 5 cut(s) 254, 327, 625, 826, 1014
BccI CCATC 6 cut(s) 166, 289, 668, 824, 1319, 2249
BciT130I CCWGG 6 cut(s) 408, 531, 1097, 1104, 1332, 1551
BciVI GTATCC 2 cut(s) 639, 1119
BcnI CCSGG 1 cut(s) 1201
BcoDI GTCTC 3 cut(s) 756, 1342, 1595
BfaI CTAG 5 cut(s) 840, 1229, 1256, 1410, 2265
BfmI CTRYAG 2 cut(s) 907, 1320
BfoI RGCGCY 3 cut(s) 594, 907, 1493
BfuAI ACCTGC 1 cut(s) 1089
BfuI GTATCC 2 cut(s) 639, 1119
BglII AGATCT 1 cut(s) 1843
Bme1390I CCNGG 7 cut(s) 408, 531, 1097, 1104, 1201, 1332, 1551
Bme18I GGWCC 9 cut(s) 149, 260, 404, 796, 1100, 1328, 1745, 1829, 1877
BmeRI GACNNNNNGTC 1 cut(s) 1334
BmeT110I CYCGRG 1 cut(s) 326
BmiI GGNNCC 6 cut(s) 679, 727, 1101, 1491, 1831, 2076
BmrFI CCNGG 7 cut(s) 408, 531, 1097, 1104, 1201, 1332, 1551
BmrI ACTGGG 1 cut(s) 2080
BmsI GCATC 3 cut(s) 277, 2043, 2270
BmuI ACTGGG 1 cut(s) 2080
BpiI GAAGAC 1 cut(s) 1045
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 221, 2201
Bpu14I TTCGAA 1 cut(s) 1698
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 1201
BsaAI YACGTR 1 cut(s) 1222
BsaHI GRCGYC 1 cut(s) 1490
BsaWI WCCGGW 3 cut(s) 146, 1034, 1460
BsaXI ACNNNNNCTCC 4 cut(s) 713, 743, 1800, 1830
Bsc4I CCNNNNNNNGG 8 cut(s) 158, 223, 613, 854, 1096, 1949, 1995, 2160
Bse118I RCCGGY 2 cut(s) 151, 1460
Bse1I ACTGG 4 cut(s) 799, 2075, 2078, 2260
Bse3DI GCAATG 1 cut(s) 1803
BseBI CCWGG 6 cut(s) 408, 531, 1097, 1104, 1332, 1551
BseGI GGATG 6 cut(s) 300, 456, 562, 679, 1058, 1767
BseLI CCNNNNNNNGG 8 cut(s) 158, 223, 613, 854, 1096, 1949, 1995, 2160
BseMI GCAATG 1 cut(s) 1803
BseMII CTCAG 2 cut(s) 1531, 2215
BseNI ACTGG 4 cut(s) 799, 2075, 2078, 2260
BseRI GAGGAG 2 cut(s) 629, 826
BseYI CCCAGC 2 cut(s) 607, 1313
BsgI GTGCAG 4 cut(s) 765, 795, 1009, 2166
BshFI GGCC 5 cut(s) 192, 245, 1095, 1662, 1802
BshNI GGYRCC 2 cut(s) 677, 1489
BshTI ACCGGT 1 cut(s) 1460
BsiHKAI GWGCWC 5 cut(s) 254, 327, 625, 826, 1014
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 8 cut(s) 147, 152, 159, 217, 570, 1035, 1200, 1461
BslFI GGGAC 7 cut(s) 1113, 1330, 1406, 1487, 1532, 1601, 1815
BslI CCNNNNNNNGG 8 cut(s) 158, 223, 613, 854, 1096, 1949, 1995, 2160
BsmAI GTCTC 3 cut(s) 756, 1342, 1595
BsmFI GGGAC 7 cut(s) 1113, 1330, 1406, 1487, 1532, 1601, 1815
BsmI GAATGC 3 cut(s) 387, 701, 1019
BsnI GGCC 5 cut(s) 192, 245, 1095, 1662, 1802
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 1698
Bsp1286I GDGCHC 5 cut(s) 254, 327, 625, 826, 1014
Bsp1407I TGTACA 3 cut(s) 208, 1855, 2193
Bsp143I GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
BspACI CCGC 3 cut(s) 203, 1571, 1579
BspANI GGCC 5 cut(s) 192, 245, 1095, 1662, 1802
BspCNI CTCAG 2 cut(s) 1530, 2214
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 6 cut(s) 679, 727, 1101, 1491, 1831, 2076
BspMAI CTGCAG 1 cut(s) 911
BspMI ACCTGC 1 cut(s) 1089
BspPI GGATC 2 cut(s) 1360, 1960
BspT104I TTCGAA 1 cut(s) 1698
BspT107I GGYRCC 2 cut(s) 677, 1489
BsrDI GCAATG 1 cut(s) 1803
BsrFI RCCGGY 2 cut(s) 151, 1460
BsrGI TGTACA 3 cut(s) 208, 1855, 2193
BsrI ACTGG 4 cut(s) 799, 2075, 2078, 2260
BssAI RCCGGY 2 cut(s) 151, 1460
BssMI GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
BssNAI GTATAC 1 cut(s) 360
BssNI GRCGYC 1 cut(s) 1490
BssT1I CCWWGG 4 cut(s) 256, 263, 550, 1803
Bst1107I GTATAC 1 cut(s) 360
Bst2UI CCWGG 6 cut(s) 408, 531, 1097, 1104, 1332, 1551
Bst4CI ACNGT 5 cut(s) 52, 627, 786, 1152, 1217
Bst6I CTCTTC 1 cut(s) 1695
BstACI GRCGYC 1 cut(s) 1490
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstAUI TGTACA 3 cut(s) 208, 1855, 2193
BstBAI YACGTR 1 cut(s) 1222
BstBI TTCGAA 1 cut(s) 1698
BstC8I GCNNGC 3 cut(s) 682, 1494, 1660
BstDEI CTNAG 5 cut(s) 221, 1124, 1388, 1517, 2201
BstDSI CCRYGG 1 cut(s) 2103
BstENI CCTNNNNNAGG 1 cut(s) 1094
BstF5I GGATG 6 cut(s) 300, 456, 562, 679, 1058, 1767
BstH2I RGCGCY 3 cut(s) 594, 907, 1493
BstHHI GCGC 4 cut(s) 593, 877, 906, 1492
BstKTI GATC 6 cut(s) 9, 123, 1171, 1368, 1846, 1955
BstMAI GTCTC 3 cut(s) 756, 1342, 1595
BstMBI GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
BstMWI GCNNNNNNNGC 5 cut(s) 296, 690, 930, 1298, 1307
BstNI CCWGG 6 cut(s) 408, 531, 1097, 1104, 1332, 1551
BstNSI RCATGY 1 cut(s) 1284
BstSCI CCNGG 7 cut(s) 406, 529, 1095, 1102, 1199, 1330, 1549
BstSFI CTRYAG 2 cut(s) 907, 1320
BstV2I GAAGAC 1 cut(s) 1045
BstX2I RGATCY 2 cut(s) 1365, 1843
BstXI CCANNNNNNTGG 2 cut(s) 1103, 1798
BstYI RGATCY 2 cut(s) 1365, 1843
BstZ17I GTATAC 1 cut(s) 360
BsuI GTATCC 2 cut(s) 639, 1119
BsuRI GGCC 5 cut(s) 192, 245, 1095, 1662, 1802
BtgI CCRYGG 1 cut(s) 2103
BtsCI GGATG 6 cut(s) 300, 456, 562, 679, 1058, 1767
BtsI GCAGTG 3 cut(s) 198, 1397, 2283
BtsIMutI CAGTG 7 cut(s) 84, 198, 763, 1026, 1397, 2253, 2283
BveI ACCTGC 1 cut(s) 1089
Cac8I GCNNGC 3 cut(s) 682, 1494, 1660
CaiI CAGNNNCTG 1 cut(s) 407
CciI TCATGA 1 cut(s) 168
CfoI GCGC 4 cut(s) 593, 877, 906, 1492
Cfr10I RCCGGY 2 cut(s) 151, 1460
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 2 cut(s) 1403, 1598
CsiI ACCWGGT 1 cut(s) 1330
Csp6I GTAC 6 cut(s) 87, 209, 1225, 1705, 1856, 2194
CspAI ACCGGT 1 cut(s) 1460
CspI CGGWCCG 1 cut(s) 149
CviAII CATG 8 cut(s) 142, 169, 187, 398, 733, 1065, 1281, 1485
CviQI GTAC 6 cut(s) 87, 209, 1225, 1705, 1856, 2194
DdeI CTNAG 5 cut(s) 221, 1124, 1388, 1517, 2201
DinI GGCGCC 1 cut(s) 1491
DpnI GATC 6 cut(s) 8, 122, 1170, 1367, 1845, 1954
DpnII GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
DraI TTTAAA 1 cut(s) 1474
DriI GACNNNNNGTC 1 cut(s) 1334
EaeI YGGCCR 3 cut(s) 243, 1093, 1800
Eam1104I CTCTTC 1 cut(s) 1695
Eam1105I GACNNNNNGTC 1 cut(s) 1334
EarI CTCTTC 1 cut(s) 1695
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 4 cut(s) 256, 263, 550, 1803
Eco147I AGGCCT 1 cut(s) 1662
Eco24I GRGCYC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 1933
Eco47I GGWCC 9 cut(s) 149, 260, 404, 796, 1100, 1328, 1745, 1829, 1877
Eco47III AGCGCT 2 cut(s) 592, 905
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 1170
Eco72I CACGTG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 252
EcoNI CCTNNNNNAGG 1 cut(s) 1094
EcoO109I RGGNCCY 3 cut(s) 404, 1100, 1829
EcoRI GAATTC 1 cut(s) 1556
EcoRII CCWGG 6 cut(s) 406, 529, 1095, 1102, 1330, 1549
EcoRV GATATC 1 cut(s) 1933
EcoT14I CCWWGG 4 cut(s) 256, 263, 550, 1803
EcoT38I GRGCYC 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 1491
EheI GGCGCC 1 cut(s) 1491
ErhI CCWWGG 4 cut(s) 256, 263, 550, 1803
FaeI CATG 8 cut(s) 145, 172, 190, 401, 736, 1068, 1284, 1488
FalI AAGNNNNNCTT 2 cut(s) 1857, 1889
FaqI GGGAC 7 cut(s) 1113, 1330, 1406, 1487, 1532, 1601, 1815
FatI CATG 8 cut(s) 141, 168, 186, 397, 732, 1064, 1280, 1484
FauI CCCGC 2 cut(s) 196, 1564
FauNDI CATATG 1 cut(s) 2038
FblI GTMKAC 1 cut(s) 359
FokI GGATG 6 cut(s) 307, 443, 569, 686, 1045, 1754
FriOI GRGCYC 1 cut(s) 254
FspBI CTAG 5 cut(s) 840, 1229, 1256, 1410, 2265
GlaI GCGC 4 cut(s) 592, 876, 905, 1491
GsaI CCCAGC 2 cut(s) 611, 1317
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 3 cut(s) 594, 907, 1493
HaeIII GGCC 5 cut(s) 192, 245, 1095, 1662, 1802
HapII CCGG 8 cut(s) 147, 152, 159, 217, 570, 1035, 1200, 1461
HgaI GACGC 2 cut(s) 1403, 1598
HhaI GCGC 4 cut(s) 593, 877, 906, 1492
Hin1I GRCGYC 1 cut(s) 1490
Hin1II CATG 8 cut(s) 145, 172, 190, 401, 736, 1068, 1284, 1488
Hin6I GCGC 4 cut(s) 591, 875, 904, 1490
HinP1I GCGC 4 cut(s) 591, 875, 904, 1490
HincII GTYRAC 2 cut(s) 484, 2025
HindII GTYRAC 2 cut(s) 484, 2025
HinfI GANTC 6 cut(s) 453, 464, 583, 655, 1889, 2234
HpaII CCGG 8 cut(s) 147, 152, 159, 217, 570, 1035, 1200, 1461
HphI GGTGA 7 cut(s) 869, 949, 1285, 1367, 1475, 1552, 1637
Hpy188I TCNGA 7 cut(s) 125, 633, 821, 1016, 1189, 1520, 1961
Hpy188III TCNNGA 4 cut(s) 169, 284, 328, 1112
Hpy99I CGWCG 1 cut(s) 953
HpyAV CCTTC 7 cut(s) 838, 865, 1153, 1253, 1264, 2024, 2224
HpyCH4III ACNGT 5 cut(s) 52, 627, 786, 1152, 1217
HpyCH4IV ACGT 2 cut(s) 639, 1221
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 690, 930, 1298, 1307
HpyF3I CTNAG 5 cut(s) 221, 1124, 1388, 1517, 2201
HpySE526I ACGT 2 cut(s) 639, 1221
Hsp92I GRCGYC 1 cut(s) 1490
Hsp92II CATG 8 cut(s) 145, 172, 190, 401, 736, 1068, 1284, 1488
HspAI GCGC 4 cut(s) 591, 875, 904, 1490
KasI GGCGCC 1 cut(s) 1489
Kzo9I GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
LmnI GCTCC 4 cut(s) 257, 698, 968, 1016
LweI GCATC 3 cut(s) 277, 2043, 2270
MabI ACCWGGT 1 cut(s) 1330
MaeI CTAG 5 cut(s) 840, 1229, 1256, 1410, 2265
MaeII ACGT 2 cut(s) 639, 1221
MaeIII GTNAC 6 cut(s) 176, 954, 997, 1046, 1217, 1613
MalI GATC 6 cut(s) 8, 122, 1170, 1367, 1845, 1954
MboI GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
MboII GAAGA 6 cut(s) 16, 377, 781, 955, 1050, 1712
MfeI CAATTG 2 cut(s) 489, 1497
MflI RGATCY 2 cut(s) 1365, 1843
MhlI GDGCHC 5 cut(s) 254, 327, 625, 826, 1014
MlsI TGGCCA 1 cut(s) 1802
MluNI TGGCCA 1 cut(s) 1802
Mly113I GGCGCC 1 cut(s) 1490
MlyI GAGTC 3 cut(s) 592, 649, 2228
MmeI TCCRAC 3 cut(s) 109, 656, 970
Mox20I TGGCCA 1 cut(s) 1802
MscI TGGCCA 1 cut(s) 1802
MseI TTAA 7 cut(s) 279, 312, 318, 1155, 1196, 1473, 1583
MslI CAYNNNNRTG 3 cut(s) 396, 1063, 1565
Msp20I TGGCCA 1 cut(s) 1802
MspA1I CMGCKG 3 cut(s) 708, 779, 1166
MspI CCGG 8 cut(s) 147, 152, 159, 217, 570, 1035, 1200, 1461
MspR9I CCNGG 7 cut(s) 408, 531, 1097, 1104, 1201, 1332, 1551
MssI GTTTAAAC 1 cut(s) 1474
MunI CAATTG 2 cut(s) 489, 1497
Mva1269I GAATGC 3 cut(s) 387, 701, 1019
MvaI CCWGG 6 cut(s) 408, 531, 1097, 1104, 1332, 1551
MwoI GCNNNNNNNGC 5 cut(s) 296, 690, 930, 1298, 1307
NarI GGCGCC 1 cut(s) 1490
NciI CCSGG 1 cut(s) 1201
NdeI CATATG 1 cut(s) 2038
NdeII GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
NlaIII CATG 8 cut(s) 145, 172, 190, 401, 736, 1068, 1284, 1488
NlaIV GGNNCC 6 cut(s) 679, 727, 1101, 1491, 1831, 2076
NmeAIII GCCGAG 2 cut(s) 271, 1071
NmuCI GTSAC 4 cut(s) 954, 997, 1046, 1217
NspI RCATGY 1 cut(s) 1284
NspV TTCGAA 1 cut(s) 1698
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 168
PceI AGGCCT 1 cut(s) 1662
PciI ACATGT 1 cut(s) 1280
PctI GAATGC 3 cut(s) 387, 701, 1019
PfeI GAWTC 3 cut(s) 453, 464, 1889
PflMI CCANNNNNTGG 4 cut(s) 854, 1949, 1995, 2160
PinAI ACCGGT 1 cut(s) 1460
PleI GAGTC 3 cut(s) 591, 649, 2228
PluTI GGCGCC 1 cut(s) 1493
PmaCI CACGTG 1 cut(s) 1222
PmeI GTTTAAAC 1 cut(s) 1474
PmlI CACGTG 1 cut(s) 1222
PpsI GAGTC 3 cut(s) 591, 649, 2228
Ppu21I YACGTR 1 cut(s) 1222
PpuMI RGGWCCY 3 cut(s) 404, 1100, 1829
PscI ACATGT 1 cut(s) 1280
PsiI TTATAA 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 639
Psp5II RGGWCCY 3 cut(s) 404, 1100, 1829
Psp6I CCWGG 6 cut(s) 406, 529, 1095, 1102, 1330, 1549
PspCI CACGTG 1 cut(s) 1222
PspFI CCCAGC 2 cut(s) 607, 1313
PspGI CCWGG 6 cut(s) 406, 529, 1095, 1102, 1330, 1549
PspN4I GGNNCC 6 cut(s) 679, 727, 1101, 1491, 1831, 2076
PspPPI RGGWCCY 3 cut(s) 404, 1100, 1829
PstI CTGCAG 1 cut(s) 911
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 2 cut(s) 1365, 1843
PvuII CAGCTG 3 cut(s) 708, 779, 1166
RsaI GTAC 6 cut(s) 88, 210, 1226, 1706, 1857, 2195
RsaNI GTAC 6 cut(s) 87, 209, 1225, 1705, 1856, 2194
RseI CAYNNNNRTG 3 cut(s) 396, 1063, 1565
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 7 cut(s) 279, 312, 318, 1155, 1196, 1473, 1583
Sau3AI GATC 6 cut(s) 6, 120, 1168, 1365, 1843, 1952
SchI GAGTC 3 cut(s) 592, 649, 2228
ScrFI CCNGG 7 cut(s) 408, 531, 1097, 1104, 1201, 1332, 1551
SduI GDGCHC 5 cut(s) 254, 327, 625, 826, 1014
SexAI ACCWGGT 1 cut(s) 1330
SfaNI GCATC 3 cut(s) 277, 2043, 2270
SfcI CTRYAG 2 cut(s) 907, 1320
SfoI GGCGCC 1 cut(s) 1491
Sfr274I CTCGAG 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 1698
SgrAI CRCCGGYG 1 cut(s) 1460
SinI GGWCC 9 cut(s) 149, 260, 404, 796, 1100, 1328, 1745, 1829, 1877
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 396, 1063, 1565
SmlI CTYRAG 2 cut(s) 326, 524
SmoI CTYRAG 2 cut(s) 326, 524
SseBI AGGCCT 1 cut(s) 1662
SsiI CCGC 3 cut(s) 203, 1571, 1579
SspDI GGCGCC 1 cut(s) 1489
SspI AATATT 1 cut(s) 316
SspMI CTAG 5 cut(s) 840, 1229, 1256, 1410, 2265
SstI GAGCTC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 1662
StyD4I CCNGG 7 cut(s) 406, 529, 1095, 1102, 1199, 1330, 1549
StyI CCWWGG 4 cut(s) 256, 263, 550, 1803
TaaI ACNGT 5 cut(s) 52, 627, 786, 1152, 1217
TaiI ACGT 2 cut(s) 642, 1224
TaqI TCGA 5 cut(s) 327, 471, 792, 1698, 1863
TatI WGTACW 5 cut(s) 86, 208, 1704, 1855, 2193
TfiI GAWTC 3 cut(s) 453, 464, 1889
Tru1I TTAA 7 cut(s) 279, 312, 318, 1155, 1196, 1473, 1583
Tru9I TTAA 7 cut(s) 279, 312, 318, 1155, 1196, 1473, 1583
TscAI CASTG 7 cut(s) 84, 205, 763, 1033, 1404, 2260, 2283
TseFI GTSAC 4 cut(s) 954, 997, 1046, 1217
Tsp45I GTSAC 4 cut(s) 954, 997, 1046, 1217
TspDTI ATGAA 8 cut(s) 17, 226, 445, 532, 1131, 1650, 1937, 2205
TspGWI ACGGA 3 cut(s) 738, 1253, 2092
TspRI CASTG 7 cut(s) 84, 205, 763, 1033, 1404, 2260, 2283
Van91I CCANNNNNTGG 4 cut(s) 854, 1949, 1995, 2160
VpaK11BI GGWCC 9 cut(s) 149, 260, 404, 796, 1100, 1328, 1745, 1829, 1877
XagI CCTNNNNNAGG 1 cut(s) 1094
XapI RAATTY 5 cut(s) 17, 1556, 1628, 1775, 2182
XceI RCATGY 1 cut(s) 1284
XcmI CCANNNNNNNNNTGG 3 cut(s) 404, 692, 986
XhoI CTCGAG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 5 cut(s) 840, 1229, 1256, 1410, 2265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.