RchiOBHm_Chr6g0304021

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017388
Physical Location & Seq
Reverse (-)
63500014 .. 63502931
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27313

Sequence Viewer

Length: 1830 bp
ATGGTATTGATGAAATTTAAATCAAACATGAAGATCAAAAGCAAATTTCCTCACACAAGGACACTCTTATCACTTACTGTTGTTGCTATATGTGTTATAAAATCAGTGAGTTGTAGTTCTACTACTGATGATAATGGCAAGTATTTGATCGGAGTTGGAAGCTATGACATGACCGGACCGGCAGCCGGAGTGGTCATGATGGGTTACGCCAACATGGGCCAAACCACTGCGGGTGTACATTTCCGGCTAAGGGCAAGGACTTTCATCGTGGCCGAGAGCTCCCAAGGTCCAAGGTTTGCCTTTGTTAATCTTGATGCAGGGATGGCTTCACAACTCGTCAATATTAAAGTGCTCGAGAAACTCAAATCAAGGTTTGGCGATTTGTATACCGAAGAAAATGTGGCGATTAGTGGCATTCACACCCATGCAGGACCTGGAGGTTATTTGCAGTATTTTCTTTACTCTATATCAACACTAGGATTCATCCAAGAATCATTCGACGTCATTGTCAACGCAATTGTGCAAAGCATTGTTCAGGCTCATCATAACCTCAAGCCTGGTTCTATTTATATCAACCAAGGGGATGTGGAAAACGCCGGAATAAACAGGAGTCCAAGCGCTTATTTGCTAAACCCAGCAGAGGAGAGAGCACAGTATCCGACCAACGTTGATACCCTAATGACTCTTTTGAAGTTTGTGGATGGTGCCAGCCAACAGAGCATTGGAGCATTCAGCTGGTTTGCCACTCACGGAACCTCCATGAGCAAAGAGAATAAGCTCATCAGTGGAGACAACAAAGGTGCAGCTGCACGGTTCTTCGAGGACCAGTTTGCTACAACTCCCATCAGAGCACCCTTCAACTCCTCTAGCGAACCAGATATTGGTGAGTTAGTAAAGAAGGCGCAGACAATCAAAGCAACAGGAGGGCAGCGCTGCAGTAAAACGAGCAGCCAAGACTGTAAGGTGAGGAAGAACGACGGGTCACTATTCGTGGGAGCTTTTTGCCAATCTAATGTTGGAGATGTGACCCCGAATGTGCTCGGAGCATTCTGCACTGATACCGGAAGACCTTGTGACTTCAGTACATCCTCATGTAATGGGAACGACCTGCTTTGCCTCGGCCGGGGACCTGGTTATCCTGATGAAATACTAAGCACCAAAATTATAGGGGAAAGACAGTTTAAGAAGGCAGCTGATCTATTTACATCGGCTTCAGAGAAATTAACCGGGGCTATTGATTACCGTCACGTGTACCTAGACTTTACGGATATTGAGGTTGAGCTAGAAGGAAACAAGAAGGTGAAAACATGTCCAGCAGCAGTTGGTGCTGGTTTTGCTGCTGGGACTACAGACGGACCTGGTGTCTCTGGGTTTCAACAAGGTGACACAGAGATCCCTGAAAAATATAGAAAACTTAGGGACGCACTGCAAAAACCTAGCGAATATCAAGTGGACTGCCAAGAACCCAAGAATGTTCTGCTTTCCACCGGTGAAATGTTTAAACCTTACGCATGGGCGCCTGCAATTGTCCCTATACAAATGCTCAGATTGGGGAAACTCATCATACTTTCTGTCCCAGGAGAATTCACCACAATGGCGGGGAGGCGGCTAAGGGACGCAGTAAAGGAGACATTGATAAGTAACAGCAAGGGTGAATTTGATGAAAACACCCACATAGTAATAGCAGGCCTTACAAATACTTATTCCCAATATATAGCAACTTTTGAAGAGTACACACAACAGAGATATGAGGTACATACTCAACCTAAAAAGAAGAACTCATCTCTTCAATATGTTTGGCATGAGATTCACCTAAATGGTCCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

609

Amino Acids

66.41

Weight (kDa)

7.9

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 48 - 584 3.8e-204 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AasI GACNNNNNNGTC 1 cut(s) 506
AatII GACGTC 1 cut(s) 504
Acc36I ACCTGC 1 cut(s) 1116
AccB1I GGYRCC 2 cut(s) 704, 1516
AccB7I CCANNNNNTGG 1 cut(s) 881
AccI GTMKAC 1 cut(s) 386
AciI CCGC 3 cut(s) 230, 1598, 1606
AclI AACGTT 1 cut(s) 666
AclWI GGATC 1 cut(s) 1387
AcoI YGGCCR 2 cut(s) 270, 1120
AcsI RAATTY 4 cut(s) 14, 44, 1583, 1655
AcuI CTGAAG 2 cut(s) 1063, 1197
AcvI CACGTG 1 cut(s) 1249
AcyI GRCGYC 2 cut(s) 501, 1517
AfaI GTAC 5 cut(s) 237, 1084, 1253, 1733, 1755
AfeI AGCGCT 2 cut(s) 619, 932
AfiI CCNNNNNNNGG 5 cut(s) 185, 250, 640, 881, 1123
AflIII ACRYGT 2 cut(s) 1248, 1307
AgeI ACCGGT 1 cut(s) 1487
AgsI TTSAA 5 cut(s) 691, 859, 1376, 1727, 1790
AhdI GACNNNNNGTC 1 cut(s) 1361
AjnI CCWGG 6 cut(s) 433, 556, 1129, 1357, 1576, 1822
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AluBI AGCT 8 cut(s) 162, 279, 735, 778, 806, 998, 1193, 1282
AluI AGCT 8 cut(s) 162, 279, 735, 778, 806, 998, 1193, 1282
Alw21I GWGCWC 5 cut(s) 281, 354, 652, 853, 1041
Alw26I GTCTC 3 cut(s) 783, 1369, 1622
AlwI GGATC 1 cut(s) 1387
AlwNI CAGNNNCTG 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 353
Aor51HI AGCGCT 2 cut(s) 619, 932
AoxI GGCC 4 cut(s) 217, 270, 1120, 1687
ApeKI GCWGC 9 cut(s) 182, 803, 806, 928, 933, 948, 1190, 1316, 1337
ApoI RAATTY 4 cut(s) 14, 44, 1583, 1655
AsiGI ACCGGT 1 cut(s) 1487
AspLEI GCGC 4 cut(s) 620, 904, 933, 1519
AspS9I GGNCC 8 cut(s) 176, 217, 287, 431, 823, 1127, 1355, 1820
AsuC2I CCSGG 2 cut(s) 1124, 1228
AsuHPI GGTGA 8 cut(s) 896, 976, 1312, 1394, 1502, 1579, 1664, 1802
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 7 cut(s) 176, 287, 431, 823, 1127, 1355, 1820
BanI GGYRCC 2 cut(s) 704, 1516
BanII GRGCYC 1 cut(s) 281
BbrPI CACGTG 1 cut(s) 1249
BbsI GAAGAC 1 cut(s) 1072
Bbv12I GWGCWC 5 cut(s) 281, 354, 652, 853, 1041
BbvI GCAGC 9 cut(s) 194, 793, 815, 920, 940, 960, 1202, 1324, 1328
BccI CCATC 4 cut(s) 193, 316, 695, 851
BciT130I CCWGG 6 cut(s) 435, 558, 1131, 1359, 1578, 1824
BciVI GTATCC 1 cut(s) 666
BcnI CCSGG 2 cut(s) 1124, 1228
BcoDI GTCTC 3 cut(s) 783, 1369, 1622
BfaI CTAG 5 cut(s) 476, 867, 1256, 1283, 1437
BfmI CTRYAG 2 cut(s) 934, 1347
BfoI RGCGCY 3 cut(s) 621, 934, 1520
BfuAI ACCTGC 1 cut(s) 1116
BfuI GTATCC 1 cut(s) 666
Bme1390I CCNGG 8 cut(s) 435, 558, 1124, 1131, 1228, 1359, 1578, 1824
Bme18I GGWCC 7 cut(s) 176, 287, 431, 823, 1127, 1355, 1820
BmeRI GACNNNNNGTC 1 cut(s) 1361
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 8 cut(s) 176, 217, 287, 431, 823, 1127, 1355, 1820
BmiI GGNNCC 4 cut(s) 706, 754, 1128, 1518
BmrFI CCNGG 8 cut(s) 435, 558, 1124, 1131, 1228, 1359, 1578, 1824
BmsI GCATC 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 1072
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 2 cut(s) 248, 1610
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 2 cut(s) 1124, 1228
BsaAI YACGTR 1 cut(s) 1249
BsaHI GRCGYC 2 cut(s) 501, 1517
BsaJI CCNNGG 8 cut(s) 283, 290, 577, 1117, 1123, 1227, 1576, 1823
BsaWI WCCGGW 3 cut(s) 173, 1061, 1487
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 250, 640, 881, 1123
Bse118I RCCGGY 2 cut(s) 178, 1487
Bse1I ACTGG 1 cut(s) 826
BseBI CCWGG 6 cut(s) 435, 558, 1131, 1359, 1578, 1824
BseDI CCNNGG 8 cut(s) 283, 290, 577, 1117, 1123, 1227, 1576, 1823
BseGI GGATG 5 cut(s) 327, 483, 589, 706, 1085
BseLI CCNNNNNNNGG 5 cut(s) 185, 250, 640, 881, 1123
BseMII CTCAG 1 cut(s) 1558
BseNI ACTGG 1 cut(s) 826
BseRI GAGGAG 2 cut(s) 656, 853
BseX3I CGGCCG 1 cut(s) 1120
BseXI GCAGC 9 cut(s) 194, 793, 815, 920, 940, 960, 1202, 1324, 1328
BseYI CCCAGC 2 cut(s) 634, 1340
BsgI GTGCAG 3 cut(s) 792, 822, 1036
Bsh1285I CGRYCG 1 cut(s) 1123
BshFI GGCC 4 cut(s) 219, 272, 1122, 1689
BshNI GGYRCC 2 cut(s) 704, 1516
BshTI ACCGGT 1 cut(s) 1487
BsiEI CGRYCG 1 cut(s) 1123
BsiHKAI GWGCWC 5 cut(s) 281, 354, 652, 853, 1041
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 9 cut(s) 174, 179, 186, 244, 597, 1062, 1123, 1227, 1488
BslFI GGGAC 6 cut(s) 1140, 1357, 1433, 1514, 1559, 1628
BslI CCNNNNNNNGG 5 cut(s) 185, 250, 640, 881, 1123
BsmAI GTCTC 3 cut(s) 783, 1369, 1622
BsmFI GGGAC 6 cut(s) 1140, 1357, 1433, 1514, 1559, 1628
BsmI GAATGC 3 cut(s) 414, 728, 1046
BsnI GGCC 4 cut(s) 219, 272, 1122, 1689
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 5 cut(s) 281, 354, 652, 853, 1041
Bsp1407I TGTACA 1 cut(s) 235
Bsp143I GATC 4 cut(s) 33, 147, 1195, 1392
BspACI CCGC 3 cut(s) 230, 1598, 1606
BspANI GGCC 4 cut(s) 219, 272, 1122, 1689
BspCNI CTCAG 1 cut(s) 1557
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 4 cut(s) 706, 754, 1128, 1518
BspMAI CTGCAG 1 cut(s) 938
BspMI ACCTGC 1 cut(s) 1116
BspPI GGATC 1 cut(s) 1387
BspT107I GGYRCC 2 cut(s) 704, 1516
BsrFI RCCGGY 2 cut(s) 178, 1487
BsrGI TGTACA 1 cut(s) 235
BsrI ACTGG 1 cut(s) 826
BssAI RCCGGY 2 cut(s) 178, 1487
BssECI CCNNGG 8 cut(s) 283, 290, 577, 1117, 1123, 1227, 1576, 1823
BssMI GATC 4 cut(s) 33, 147, 1195, 1392
BssNAI GTATAC 1 cut(s) 387
BssNI GRCGYC 2 cut(s) 501, 1517
BssT1I CCWWGG 3 cut(s) 283, 290, 577
Bst1107I GTATAC 1 cut(s) 387
Bst2UI CCWGG 6 cut(s) 435, 558, 1131, 1359, 1578, 1824
Bst4CI ACNGT 6 cut(s) 79, 654, 813, 959, 1179, 1244
Bst6I CTCTTC 2 cut(s) 1722, 1791
BstACI GRCGYC 2 cut(s) 501, 1517
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstAUI TGTACA 1 cut(s) 235
BstBAI YACGTR 1 cut(s) 1249
BstC8I GCNNGC 3 cut(s) 709, 1521, 1687
BstDEI CTNAG 5 cut(s) 248, 1151, 1415, 1544, 1610
BstF5I GGATG 5 cut(s) 327, 483, 589, 706, 1085
BstH2I RGCGCY 3 cut(s) 621, 934, 1520
BstHHI GCGC 4 cut(s) 620, 904, 933, 1519
BstKTI GATC 4 cut(s) 36, 150, 1198, 1395
BstMAI GTCTC 3 cut(s) 783, 1369, 1622
BstMBI GATC 4 cut(s) 33, 147, 1195, 1392
BstMCI CGRYCG 1 cut(s) 1123
BstMWI GCNNNNNNNGC 4 cut(s) 323, 717, 1325, 1334
BstNI CCWGG 6 cut(s) 435, 558, 1131, 1359, 1578, 1824
BstNSI RCATGY 1 cut(s) 1311
BstSCI CCNGG 8 cut(s) 433, 556, 1122, 1129, 1226, 1357, 1576, 1822
BstSFI CTRYAG 2 cut(s) 934, 1347
BstV1I GCAGC 9 cut(s) 194, 793, 815, 920, 940, 960, 1202, 1324, 1328
BstV2I GAAGAC 1 cut(s) 1072
BstX2I RGATCY 1 cut(s) 1392
BstYI RGATCY 1 cut(s) 1392
BstZ17I GTATAC 1 cut(s) 387
BstZI CGGCCG 1 cut(s) 1120
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 4 cut(s) 219, 272, 1122, 1689
BtsCI GGATG 5 cut(s) 327, 483, 589, 706, 1085
BtsI GCAGTG 2 cut(s) 225, 1424
BtsIMutI CAGTG 5 cut(s) 111, 225, 790, 1053, 1424
BveI ACCTGC 1 cut(s) 1116
Cac8I GCNNGC 3 cut(s) 709, 1521, 1687
CaiI CAGNNNCTG 1 cut(s) 434
CciI TCATGA 1 cut(s) 195
CfoI GCGC 4 cut(s) 620, 904, 933, 1519
Cfr10I RCCGGY 2 cut(s) 178, 1487
Cfr13I GGNCC 8 cut(s) 176, 217, 287, 431, 823, 1127, 1355, 1820
CpoI CGGWCCG 1 cut(s) 176
CseI GACGC 2 cut(s) 1430, 1625
CsiI ACCWGGT 2 cut(s) 1129, 1357
Csp6I GTAC 5 cut(s) 236, 1083, 1252, 1732, 1754
CspAI ACCGGT 1 cut(s) 1487
CspI CGGWCCG 1 cut(s) 176
CviQI GTAC 5 cut(s) 236, 1083, 1252, 1732, 1754
DdeI CTNAG 5 cut(s) 248, 1151, 1415, 1544, 1610
DinI GGCGCC 1 cut(s) 1518
DpnI GATC 4 cut(s) 35, 149, 1197, 1394
DpnII GATC 4 cut(s) 33, 147, 1195, 1392
DraI TTTAAA 2 cut(s) 19, 1501
DrdI GACNNNNNNGTC 1 cut(s) 506
DriI GACNNNNNGTC 1 cut(s) 1361
DseDI GACNNNNNNGTC 1 cut(s) 506
EaeI YGGCCR 2 cut(s) 270, 1120
EagI CGGCCG 1 cut(s) 1120
Eam1104I CTCTTC 2 cut(s) 1722, 1791
Eam1105I GACNNNNNGTC 1 cut(s) 1361
EarI CTCTTC 2 cut(s) 1722, 1791
Ecl136II GAGCTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 1120
Eco130I CCWWGG 3 cut(s) 283, 290, 577
Eco147I AGGCCT 1 cut(s) 1689
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 7 cut(s) 176, 287, 431, 823, 1127, 1355, 1820
Eco47III AGCGCT 2 cut(s) 619, 932
Eco52I CGGCCG 1 cut(s) 1120
Eco53kI GAGCTC 1 cut(s) 279
Eco57I CTGAAG 2 cut(s) 1063, 1197
Eco72I CACGTG 1 cut(s) 1249
Eco88I CYCGRG 1 cut(s) 353
EcoICRI GAGCTC 1 cut(s) 279
EcoO109I RGGNCCY 2 cut(s) 431, 1127
EcoRI GAATTC 1 cut(s) 1583
EcoRII CCWGG 6 cut(s) 433, 556, 1129, 1357, 1576, 1822
EcoT14I CCWWGG 3 cut(s) 283, 290, 577
EcoT38I GRGCYC 1 cut(s) 281
EgeI GGCGCC 1 cut(s) 1518
EheI GGCGCC 1 cut(s) 1518
ErhI CCWWGG 3 cut(s) 283, 290, 577
FaqI GGGAC 6 cut(s) 1140, 1357, 1433, 1514, 1559, 1628
FauI CCCGC 2 cut(s) 223, 1591
FblI GTMKAC 1 cut(s) 386
FokI GGATG 5 cut(s) 334, 470, 596, 713, 1072
FriOI GRGCYC 1 cut(s) 281
FspBI CTAG 5 cut(s) 476, 867, 1256, 1283, 1437
GlaI GCGC 4 cut(s) 619, 903, 932, 1518
GsaI CCCAGC 2 cut(s) 638, 1344
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 3 cut(s) 621, 934, 1520
HaeIII GGCC 4 cut(s) 219, 272, 1122, 1689
HapII CCGG 9 cut(s) 174, 179, 186, 244, 597, 1062, 1123, 1227, 1488
HgaI GACGC 2 cut(s) 1430, 1625
HhaI GCGC 4 cut(s) 620, 904, 933, 1519
Hin1I GRCGYC 2 cut(s) 501, 1517
Hin6I GCGC 4 cut(s) 618, 902, 931, 1517
HinP1I GCGC 4 cut(s) 618, 902, 931, 1517
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 5 cut(s) 480, 491, 610, 682, 1807
HpaII CCGG 9 cut(s) 174, 179, 186, 244, 597, 1062, 1123, 1227, 1488
HphI GGTGA 8 cut(s) 896, 976, 1312, 1394, 1502, 1579, 1664, 1802
Hpy166II GTNNAC 6 cut(s) 236, 387, 511, 1252, 1453, 1734
Hpy188I TCNGA 6 cut(s) 152, 660, 848, 1043, 1216, 1547
Hpy188III TCNNGA 4 cut(s) 196, 311, 355, 1139
Hpy8I GTNNAC 6 cut(s) 236, 387, 511, 1252, 1453, 1734
Hpy99I CGWCG 2 cut(s) 503, 980
HpyAV CCTTC 5 cut(s) 865, 892, 1180, 1280, 1291
HpyCH4III ACNGT 6 cut(s) 79, 654, 813, 959, 1179, 1244
HpyCH4IV ACGT 3 cut(s) 501, 666, 1248
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 717, 1325, 1334
HpyF3I CTNAG 5 cut(s) 248, 1151, 1415, 1544, 1610
HpySE526I ACGT 3 cut(s) 501, 666, 1248
Hsp92I GRCGYC 2 cut(s) 501, 1517
HspAI GCGC 4 cut(s) 618, 902, 931, 1517
KasI GGCGCC 1 cut(s) 1516
Kzo9I GATC 4 cut(s) 33, 147, 1195, 1392
LmnI GCTCC 4 cut(s) 284, 725, 995, 1043
Lsp1109I GCAGC 9 cut(s) 194, 793, 815, 920, 940, 960, 1202, 1324, 1328
LweI GCATC 1 cut(s) 304
MabI ACCWGGT 2 cut(s) 1129, 1357
MaeI CTAG 5 cut(s) 476, 867, 1256, 1283, 1437
MaeII ACGT 3 cut(s) 501, 666, 1248
MaeIII GTNAC 7 cut(s) 203, 981, 1024, 1073, 1244, 1382, 1640
MalI GATC 4 cut(s) 35, 149, 1197, 1394
MboI GATC 4 cut(s) 33, 147, 1195, 1392
MboII GAAGA 8 cut(s) 43, 404, 808, 982, 1077, 1739, 1778, 1786
MfeI CAATTG 2 cut(s) 516, 1524
MflI RGATCY 1 cut(s) 1392
MhlI GDGCHC 5 cut(s) 281, 354, 652, 853, 1041
MluCI AATT 8 cut(s) 14, 44, 516, 1161, 1220, 1524, 1583, 1655
Mly113I GGCGCC 1 cut(s) 1517
MlyI GAGTC 2 cut(s) 619, 676
MmeI TCCRAC 3 cut(s) 136, 683, 997
MseI TTAA 6 cut(s) 18, 306, 345, 1182, 1223, 1500
MslI CAYNNNNRTG 4 cut(s) 423, 1090, 1592, 1815
MspA1I CMGCKG 3 cut(s) 735, 806, 1193
MspI CCGG 9 cut(s) 174, 179, 186, 244, 597, 1062, 1123, 1227, 1488
MspR9I CCNGG 8 cut(s) 435, 558, 1124, 1131, 1228, 1359, 1578, 1824
MssI GTTTAAAC 1 cut(s) 1501
MunI CAATTG 2 cut(s) 516, 1524
Mva1269I GAATGC 3 cut(s) 414, 728, 1046
MvaI CCWGG 6 cut(s) 435, 558, 1131, 1359, 1578, 1824
MwoI GCNNNNNNNGC 4 cut(s) 323, 717, 1325, 1334
NarI GGCGCC 1 cut(s) 1517
NciI CCSGG 2 cut(s) 1124, 1228
NdeII GATC 4 cut(s) 33, 147, 1195, 1392
NlaIV GGNNCC 4 cut(s) 706, 754, 1128, 1518
NmeAIII GCCGAG 2 cut(s) 298, 1098
NmuCI GTSAC 5 cut(s) 981, 1024, 1073, 1244, 1382
NspI RCATGY 1 cut(s) 1311
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 1 cut(s) 195
PceI AGGCCT 1 cut(s) 1689
PciI ACATGT 1 cut(s) 1307
PctI GAATGC 3 cut(s) 414, 728, 1046
PfeI GAWTC 3 cut(s) 480, 491, 1807
PflMI CCANNNNNTGG 1 cut(s) 881
PinAI ACCGGT 1 cut(s) 1487
PleI GAGTC 2 cut(s) 618, 676
PluTI GGCGCC 1 cut(s) 1520
PmaCI CACGTG 1 cut(s) 1249
PmeI GTTTAAAC 1 cut(s) 1501
PmlI CACGTG 1 cut(s) 1249
PpsI GAGTC 2 cut(s) 618, 676
Ppu21I YACGTR 1 cut(s) 1249
PpuMI RGGWCCY 2 cut(s) 431, 1127
PscI ACATGT 1 cut(s) 1307
PsiI TTATAA 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 281
Psp1406I AACGTT 1 cut(s) 666
Psp5II RGGWCCY 2 cut(s) 431, 1127
Psp6I CCWGG 6 cut(s) 433, 556, 1129, 1357, 1576, 1822
PspCI CACGTG 1 cut(s) 1249
PspFI CCCAGC 2 cut(s) 634, 1340
PspGI CCWGG 6 cut(s) 433, 556, 1129, 1357, 1576, 1822
PspN4I GGNNCC 4 cut(s) 706, 754, 1128, 1518
PspPI GGNCC 8 cut(s) 176, 217, 287, 431, 823, 1127, 1355, 1820
PspPPI RGGWCCY 2 cut(s) 431, 1127
PstI CTGCAG 1 cut(s) 938
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 1392
PvuII CAGCTG 3 cut(s) 735, 806, 1193
RsaI GTAC 5 cut(s) 237, 1084, 1253, 1733, 1755
RsaNI GTAC 5 cut(s) 236, 1083, 1252, 1732, 1754
RseI CAYNNNNRTG 4 cut(s) 423, 1090, 1592, 1815
Rsr2I CGGWCCG 1 cut(s) 176
RsrII CGGWCCG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 281
SaqAI TTAA 6 cut(s) 18, 306, 345, 1182, 1223, 1500
Sau3AI GATC 4 cut(s) 33, 147, 1195, 1392
Sau96I GGNCC 8 cut(s) 176, 217, 287, 431, 823, 1127, 1355, 1820
SchI GAGTC 2 cut(s) 619, 676
ScrFI CCNGG 8 cut(s) 435, 558, 1124, 1131, 1228, 1359, 1578, 1824
SduI GDGCHC 5 cut(s) 281, 354, 652, 853, 1041
SexAI ACCWGGT 2 cut(s) 1129, 1357
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 2 cut(s) 934, 1347
SfoI GGCGCC 1 cut(s) 1518
Sfr274I CTCGAG 1 cut(s) 353
SgrAI CRCCGGYG 1 cut(s) 1487
SinI GGWCC 7 cut(s) 176, 287, 431, 823, 1127, 1355, 1820
SlaI CTCGAG 1 cut(s) 353
SmiI ATTTAAAT 1 cut(s) 19
SmiMI CAYNNNNRTG 4 cut(s) 423, 1090, 1592, 1815
SmlI CTYRAG 2 cut(s) 353, 551
SmoI CTYRAG 2 cut(s) 353, 551
Sse9I AATT 8 cut(s) 14, 44, 516, 1161, 1220, 1524, 1583, 1655
SseBI AGGCCT 1 cut(s) 1689
SsiI CCGC 3 cut(s) 230, 1598, 1606
SspDI GGCGCC 1 cut(s) 1516
SspI AATATT 1 cut(s) 343
SspMI CTAG 5 cut(s) 476, 867, 1256, 1283, 1437
SstI GAGCTC 1 cut(s) 281
StuI AGGCCT 1 cut(s) 1689
StyD4I CCNGG 8 cut(s) 433, 556, 1122, 1129, 1226, 1357, 1576, 1822
StyI CCWWGG 3 cut(s) 283, 290, 577
SwaI ATTTAAAT 1 cut(s) 19
TaaI ACNGT 6 cut(s) 79, 654, 813, 959, 1179, 1244
TaiI ACGT 3 cut(s) 504, 669, 1251
TaqI TCGA 3 cut(s) 354, 498, 819
TasI AATT 8 cut(s) 14, 44, 516, 1161, 1220, 1524, 1583, 1655
TatI WGTACW 3 cut(s) 235, 1082, 1731
TauI GCSGC 1 cut(s) 1609
TfiI GAWTC 3 cut(s) 480, 491, 1807
Tru1I TTAA 6 cut(s) 18, 306, 345, 1182, 1223, 1500
Tru9I TTAA 6 cut(s) 18, 306, 345, 1182, 1223, 1500
TscAI CASTG 5 cut(s) 111, 232, 790, 1060, 1431
TseFI GTSAC 5 cut(s) 981, 1024, 1073, 1244, 1382
TseI GCWGC 9 cut(s) 182, 803, 806, 928, 933, 948, 1190, 1316, 1337
Tsp45I GTSAC 5 cut(s) 981, 1024, 1073, 1244, 1382
TspDTI ATGAA 6 cut(s) 26, 44, 253, 472, 1158, 1677
TspGWI ACGGA 3 cut(s) 765, 1280, 1368
TspRI CASTG 5 cut(s) 111, 232, 790, 1060, 1431
Van91I CCANNNNNTGG 1 cut(s) 881
VpaK11BI GGWCC 7 cut(s) 176, 287, 431, 823, 1127, 1355, 1820
XapI RAATTY 4 cut(s) 14, 44, 1583, 1655
XceI RCATGY 1 cut(s) 1311
XcmI CCANNNNNNNNNTGG 3 cut(s) 431, 719, 1013
XhoI CTCGAG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 386
XspI CTAG 5 cut(s) 476, 867, 1256, 1283, 1437
ZraI GACGTC 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.