Rmu_sc0003805.1_g000016

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003805.1
OG0017388
Physical Location & Seq
Forward (+)
70758 .. 74259
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003805.1_g000016.1.cds

Sequence Viewer

Length: 2190 bp
atgaagatcaaaagcaaatttcctcacacaaggacacccttatcacttactgttgttgctatatgtgttataaaatcagtgagttgtagtactaaaattgatcataatggcaagtatttgatcggagttggaagctatgacatgaccggaccggcagccggagtggtcatgatgggttacgccaacatgggccaaaccactgcaggtgtacatttccggttaagggcaaggactttcatcgtggccgagagctcccaaggtccaagggttgcctttgttaatcttgatgcagggatggcttcacaactcgtcaatattaaagtgctcgagaaactcaaatcaaggtttggcgatttgtataccgaagaaaatgtggcgattagtggcattcacacccatgcaggacctggaggttatttgcagtattttctctactctatatcaacactaggattcatccaagaatcattcgacgtcattgtcaacgcaattgtgcaaagcattgttcaggctcatcataacctcaagcctggggatgtggtaaacgccggaataaacaggagtccaagcgcttatttgctaaacccagcagaggagagagcacagtatccgacgaacgttgataccctaatgactcttttgaagtttgtggatggtgccagccaacagagcattggagcattcagctggtttgccactcacggaacctccatgagcaaagagaataagctcatcagtggagacaacaaaggtgcagctgcacggttctttgaggaccagtttgctacaactcccatcagagcacccttcaactcctctagcaaagcagatattggtgagttagtaaagaaggcgcagacaatcaaagcaacaggagggcagcgctgcagtaaaacgagcagccaagactgtaaggtgaggaagaacgacgggtcactattcgtgggagctttttgccaatctaatgttggagatgtgaccccgaatgtgctcggagcattctgcactgataccggaagaccttgtgacttcaatacatcctcatgtaatgggaacgacctgctttgcgtcggccggggacctgggtatcctgatgaaagactaagcaccaaaattataggggaaagacagtttaagaaggcagctgatctatttacatcggcttcagagaaattaacgggggctattgattaccgtcacgtgtacctagactttacggatattgaggttgagctagaaggaaacaagaaggtgaaaacatgtccagcagcagttggtgctggttttgctgctgggactacagatggacctggtgtctctgggtttcaacaaggtgatacagagatccctgaaaaatatagaaaacttagggacgcactgcaaaaacctagtgaatatcaagtggactgccaagaacccaagaatgttctgctttccaccggtgaaatgtttaaaccttacgcatgggcgcctgcaattgtccctatacaaatgctcagattggggaaactcatcatactttctgtcccaggagaattcaccacaatggcggggaggcggctaagggacgcagtaaaggagacattgataagtaacagcaagggtgaatttgatgaaaacacccacatagctgcttccactctctatggtcctcacacattatcagcatacatccaagaatttgagaaactagccaaagcaatggccaagggagaaaaggtcatttctaaaggtccctcgccaccagatctttcctctgtacaagttgaagtcttacttggaccttttggagattcgcctccaccacatacaaagtttggagatatgaaacaagatatcattataccaaaaagtggttcattcagaaagggagatttgaatagaccaaatgccacattttggagtgcaaacccaaactatgacttgttgacagaaggcacatatgctgtggtagagatgcttcaaggggataactgggttccagtttacgatgatgatgatttctccgtggttttcaagtggaatagagatgacaagggattatatagtgcagcaaccatagaatgggaaataccaatttatgcaaatttgggggtgtacaggctcaggcattttggttcatcaaggaaaaagaaggactcccccatcatctacttcactggtgcgtctagtgggtttgcagtgtcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

729

Amino Acids

79.27

Weight (kDa)

8.28

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AarI CACCTGC 1 cut(s) 194
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 2 cut(s) 194, 1068
AccB1I GGYRCC 2 cut(s) 656, 1468
AccB7I CCANNNNNTGG 3 cut(s) 1853, 1899, 2064
AccI GTMKAC 1 cut(s) 359
AciI CCGC 2 cut(s) 1550, 1558
AclI AACGTT 1 cut(s) 618
AclWI GGATC 1 cut(s) 1339
AcoI YGGCCR 3 cut(s) 243, 1072, 1704
AcsI RAATTY 5 cut(s) 17, 1535, 1607, 1679, 2086
AcuI CTGAAG 1 cut(s) 1149
AcvI CACGTG 1 cut(s) 1201
AcyI GRCGYC 2 cut(s) 474, 1469
AfaI GTAC 5 cut(s) 91, 210, 1205, 1761, 2099
AfeI AGCGCT 2 cut(s) 571, 884
AfiI CCNNNNNNNGG 6 cut(s) 158, 223, 592, 1853, 1899, 2064
AflIII ACRYGT 2 cut(s) 1200, 1259
AgeI ACCGGT 1 cut(s) 1439
AgsI TTSAA 8 cut(s) 643, 811, 1033, 1328, 1769, 1879, 1964, 2017
AhdI GACNNNNNGTC 1 cut(s) 1313
AjnI CCWGG 5 cut(s) 406, 529, 1081, 1309, 1528
AjuI GAANNNNNNNTTGG 4 cut(s) 1761, 1793, 1840, 1872
AluBI AGCT 9 cut(s) 135, 252, 687, 730, 758, 950, 1145, 1234, 1631
AluI AGCT 9 cut(s) 135, 252, 687, 730, 758, 950, 1145, 1234, 1631
Alw21I GWGCWC 5 cut(s) 254, 327, 604, 805, 993
Alw26I GTCTC 3 cut(s) 735, 1321, 1574
AlwI GGATC 1 cut(s) 1339
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 326
Aor51HI AGCGCT 2 cut(s) 571, 884
AoxI GGCC 4 cut(s) 190, 243, 1072, 1704
ApoI RAATTY 5 cut(s) 17, 1535, 1607, 1679, 2086
AsiGI ACCGGT 1 cut(s) 1439
AspLEI GCGC 4 cut(s) 572, 856, 885, 1471
AsuC2I CCSGG 1 cut(s) 1076
AsuHPI GGTGA 7 cut(s) 848, 928, 1264, 1346, 1454, 1531, 1616
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 9 cut(s) 149, 260, 404, 775, 1079, 1307, 1649, 1733, 1781
BaeI ACNNNNGTAYC 2 cut(s) 1070, 1103
BalI TGGCCA 1 cut(s) 1706
BanI GGYRCC 2 cut(s) 656, 1468
BanII GRGCYC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 1944, 1976
BbrPI CACGTG 1 cut(s) 1201
BbsI GAAGAC 1 cut(s) 1024
Bbv12I GWGCWC 5 cut(s) 254, 327, 604, 805, 993
BccI CCATC 6 cut(s) 166, 289, 647, 803, 1298, 2153
BciT130I CCWGG 5 cut(s) 408, 531, 1083, 1311, 1530
BciVI GTATCC 2 cut(s) 618, 1098
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 1 cut(s) 1076
BcoDI GTCTC 3 cut(s) 735, 1321, 1574
BfaI CTAG 7 cut(s) 449, 819, 1208, 1235, 1389, 1691, 2169
BfmI CTRYAG 3 cut(s) 201, 886, 1299
BfoI RGCGCY 3 cut(s) 573, 886, 1472
BfuAI ACCTGC 2 cut(s) 194, 1068
BfuI GTATCC 2 cut(s) 618, 1098
BglII AGATCT 1 cut(s) 1747
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 6 cut(s) 408, 531, 1076, 1083, 1311, 1530
Bme18I GGWCC 9 cut(s) 149, 260, 404, 775, 1079, 1307, 1649, 1733, 1781
BmeRI GACNNNNNGTC 1 cut(s) 1313
BmeT110I CYCGRG 1 cut(s) 326
BmiI GGNNCC 6 cut(s) 658, 706, 1080, 1470, 1735, 1980
BmrFI CCNGG 6 cut(s) 408, 531, 1076, 1083, 1311, 1530
BmrI ACTGGG 1 cut(s) 1984
BmsI GCATC 2 cut(s) 277, 1947
BmuI ACTGGG 1 cut(s) 1984
BpiI GAAGAC 1 cut(s) 1024
BpmI CTGGAG 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 1562, 2105
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 1076
BsaAI YACGTR 1 cut(s) 1201
BsaHI GRCGYC 2 cut(s) 474, 1469
BsaJI CCNNGG 8 cut(s) 256, 263, 530, 1075, 1082, 1528, 1707, 2007
BsaWI WCCGGW 4 cut(s) 146, 216, 1013, 1439
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 1704, 1734
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 223, 592, 1853, 1899, 2064
Bse118I RCCGGY 2 cut(s) 151, 1439
Bse1I ACTGG 4 cut(s) 778, 1979, 1982, 2164
Bse3DI GCAATG 1 cut(s) 1707
BseBI CCWGG 5 cut(s) 408, 531, 1083, 1311, 1530
BseDI CCNNGG 8 cut(s) 256, 263, 530, 1075, 1082, 1528, 1707, 2007
BseGI GGATG 6 cut(s) 300, 456, 541, 658, 1037, 1671
BseLI CCNNNNNNNGG 6 cut(s) 158, 223, 592, 1853, 1899, 2064
BseMI GCAATG 1 cut(s) 1707
BseMII CTCAG 2 cut(s) 1510, 2119
BseNI ACTGG 4 cut(s) 778, 1979, 1982, 2164
BseRI GAGGAG 2 cut(s) 608, 805
BseX3I CGGCCG 1 cut(s) 1072
BseYI CCCAGC 2 cut(s) 586, 1292
BsgI GTGCAG 4 cut(s) 744, 774, 988, 2070
Bsh1285I CGRYCG 1 cut(s) 1075
BshFI GGCC 4 cut(s) 192, 245, 1074, 1706
BshNI GGYRCC 2 cut(s) 656, 1468
BshTI ACCGGT 1 cut(s) 1439
BsiEI CGRYCG 1 cut(s) 1075
BsiHKAI GWGCWC 5 cut(s) 254, 327, 604, 805, 993
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 8 cut(s) 147, 152, 159, 217, 549, 1014, 1075, 1440
BslFI GGGAC 7 cut(s) 1092, 1309, 1385, 1466, 1511, 1580, 1719
BslI CCNNNNNNNGG 6 cut(s) 158, 223, 592, 1853, 1899, 2064
BsmAI GTCTC 3 cut(s) 735, 1321, 1574
BsmFI GGGAC 7 cut(s) 1092, 1309, 1385, 1466, 1511, 1580, 1719
BsmI GAATGC 3 cut(s) 387, 680, 998
BsnI GGCC 4 cut(s) 192, 245, 1074, 1706
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 5 cut(s) 254, 327, 604, 805, 993
Bsp1407I TGTACA 3 cut(s) 208, 1759, 2097
Bsp143I GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
BspACI CCGC 2 cut(s) 1550, 1558
BspANI GGCC 4 cut(s) 192, 245, 1074, 1706
BspCNI CTCAG 2 cut(s) 1509, 2118
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 6 cut(s) 658, 706, 1080, 1470, 1735, 1980
BspMAI CTGCAG 2 cut(s) 205, 890
BspMI ACCTGC 2 cut(s) 194, 1068
BspPI GGATC 1 cut(s) 1339
BspT107I GGYRCC 2 cut(s) 656, 1468
BsrDI GCAATG 1 cut(s) 1707
BsrFI RCCGGY 2 cut(s) 151, 1439
BsrGI TGTACA 3 cut(s) 208, 1759, 2097
BsrI ACTGG 4 cut(s) 778, 1979, 1982, 2164
BssAI RCCGGY 2 cut(s) 151, 1439
BssECI CCNNGG 8 cut(s) 256, 263, 530, 1075, 1082, 1528, 1707, 2007
BssMI GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
BssNAI GTATAC 1 cut(s) 360
BssNI GRCGYC 2 cut(s) 474, 1469
BssT1I CCWWGG 3 cut(s) 256, 263, 1707
Bst1107I GTATAC 1 cut(s) 360
Bst2UI CCWGG 5 cut(s) 408, 531, 1083, 1311, 1530
Bst4CI ACNGT 6 cut(s) 52, 606, 765, 911, 1131, 1196
BstACI GRCGYC 2 cut(s) 474, 1469
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstAUI TGTACA 3 cut(s) 208, 1759, 2097
BstBAI YACGTR 1 cut(s) 1201
BstC8I GCNNGC 2 cut(s) 661, 1473
BstDEI CTNAG 5 cut(s) 1103, 1367, 1496, 1562, 2105
BstDSI CCRYGG 1 cut(s) 2007
BstF5I GGATG 6 cut(s) 300, 456, 541, 658, 1037, 1671
BstH2I RGCGCY 3 cut(s) 573, 886, 1472
BstHHI GCGC 4 cut(s) 572, 856, 885, 1471
BstKTI GATC 6 cut(s) 9, 103, 123, 1150, 1347, 1750
BstMAI GTCTC 3 cut(s) 735, 1321, 1574
BstMBI GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
BstMCI CGRYCG 1 cut(s) 1075
BstMWI GCNNNNNNNGC 4 cut(s) 296, 669, 1277, 1286
BstNI CCWGG 5 cut(s) 408, 531, 1083, 1311, 1530
BstNSI RCATGY 1 cut(s) 1263
BstSCI CCNGG 6 cut(s) 406, 529, 1074, 1081, 1309, 1528
BstSFI CTRYAG 3 cut(s) 201, 886, 1299
BstV2I GAAGAC 1 cut(s) 1024
BstX2I RGATCY 2 cut(s) 1344, 1747
BstXI CCANNNNNNTGG 1 cut(s) 1702
BstYI RGATCY 2 cut(s) 1344, 1747
BstZ17I GTATAC 1 cut(s) 360
BstZI CGGCCG 1 cut(s) 1072
BsuI GTATCC 2 cut(s) 618, 1098
BsuRI GGCC 4 cut(s) 192, 245, 1074, 1706
BtgI CCRYGG 1 cut(s) 2007
BtsCI GGATG 6 cut(s) 300, 456, 541, 658, 1037, 1671
BtsI GCAGTG 3 cut(s) 198, 1376, 2187
BtsIMutI CAGTG 7 cut(s) 84, 198, 742, 1005, 1376, 2157, 2187
BveI ACCTGC 2 cut(s) 194, 1068
Cac8I GCNNGC 2 cut(s) 661, 1473
CaiI CAGNNNCTG 1 cut(s) 407
CciI TCATGA 1 cut(s) 168
CfoI GCGC 4 cut(s) 572, 856, 885, 1471
Cfr10I RCCGGY 2 cut(s) 151, 1439
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 4 cut(s) 1057, 1382, 1577, 2154
CsiI ACCWGGT 1 cut(s) 1309
Csp6I GTAC 5 cut(s) 90, 209, 1204, 1760, 2098
CspAI ACCGGT 1 cut(s) 1439
CspI CGGWCCG 1 cut(s) 149
CviAII CATG 8 cut(s) 142, 169, 187, 398, 712, 1044, 1260, 1464
CviQI GTAC 5 cut(s) 90, 209, 1204, 1760, 2098
DdeI CTNAG 5 cut(s) 1103, 1367, 1496, 1562, 2105
DinI GGCGCC 1 cut(s) 1470
DpnI GATC 6 cut(s) 8, 102, 122, 1149, 1346, 1749
DpnII GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
DraI TTTAAA 1 cut(s) 1453
DrdI GACNNNNNNGTC 1 cut(s) 479
DriI GACNNNNNGTC 1 cut(s) 1313
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 3 cut(s) 243, 1072, 1704
EagI CGGCCG 1 cut(s) 1072
Eam1105I GACNNNNNGTC 1 cut(s) 1313
Ecl136II GAGCTC 1 cut(s) 252
EclXI CGGCCG 1 cut(s) 1072
Eco130I CCWWGG 3 cut(s) 256, 263, 1707
Eco24I GRGCYC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 1837
Eco47I GGWCC 9 cut(s) 149, 260, 404, 775, 1079, 1307, 1649, 1733, 1781
Eco47III AGCGCT 2 cut(s) 571, 884
Eco52I CGGCCG 1 cut(s) 1072
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 1149
Eco72I CACGTG 1 cut(s) 1201
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 3 cut(s) 404, 1079, 1733
EcoRI GAATTC 1 cut(s) 1535
EcoRII CCWGG 5 cut(s) 406, 529, 1081, 1309, 1528
EcoRV GATATC 1 cut(s) 1837
EcoT14I CCWWGG 3 cut(s) 256, 263, 1707
EcoT38I GRGCYC 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 1470
EheI GGCGCC 1 cut(s) 1470
ErhI CCWWGG 3 cut(s) 256, 263, 1707
FaeI CATG 8 cut(s) 145, 172, 190, 401, 715, 1047, 1263, 1467
FalI AAGNNNNNCTT 2 cut(s) 1761, 1793
FaqI GGGAC 7 cut(s) 1092, 1309, 1385, 1466, 1511, 1580, 1719
FatI CATG 8 cut(s) 141, 168, 186, 397, 711, 1043, 1259, 1463
FauI CCCGC 1 cut(s) 1543
FauNDI CATATG 1 cut(s) 1942
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 1 cut(s) 359
FokI GGATG 6 cut(s) 307, 443, 548, 665, 1024, 1658
FriOI GRGCYC 1 cut(s) 254
FspBI CTAG 7 cut(s) 449, 819, 1208, 1235, 1389, 1691, 2169
GlaI GCGC 4 cut(s) 571, 855, 884, 1470
GsaI CCCAGC 2 cut(s) 590, 1296
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 3 cut(s) 573, 886, 1472
HaeIII GGCC 4 cut(s) 192, 245, 1074, 1706
HapII CCGG 8 cut(s) 147, 152, 159, 217, 549, 1014, 1075, 1440
HgaI GACGC 4 cut(s) 1057, 1382, 1577, 2154
HhaI GCGC 4 cut(s) 572, 856, 885, 1471
Hin1I GRCGYC 2 cut(s) 474, 1469
Hin1II CATG 8 cut(s) 145, 172, 190, 401, 715, 1047, 1263, 1467
Hin6I GCGC 4 cut(s) 570, 854, 883, 1469
HinP1I GCGC 4 cut(s) 570, 854, 883, 1469
HincII GTYRAC 2 cut(s) 484, 1929
HindII GTYRAC 2 cut(s) 484, 1929
HinfI GANTC 6 cut(s) 453, 464, 562, 634, 1793, 2138
HpaII CCGG 8 cut(s) 147, 152, 159, 217, 549, 1014, 1075, 1440
HphI GGTGA 7 cut(s) 848, 928, 1264, 1346, 1454, 1531, 1616
Hpy166II GTNNAC 9 cut(s) 209, 360, 484, 544, 1204, 1405, 1929, 1987, 2098
Hpy188I TCNGA 7 cut(s) 125, 612, 800, 995, 1168, 1499, 1865
Hpy188III TCNNGA 4 cut(s) 169, 284, 328, 1091
Hpy8I GTNNAC 9 cut(s) 209, 360, 484, 544, 1204, 1405, 1929, 1987, 2098
Hpy99I CGWCG 4 cut(s) 476, 616, 932, 1073
HpyAV CCTTC 7 cut(s) 817, 844, 1132, 1232, 1243, 1928, 2128
HpyCH4III ACNGT 6 cut(s) 52, 606, 765, 911, 1131, 1196
HpyCH4IV ACGT 3 cut(s) 474, 618, 1200
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 669, 1277, 1286
HpyF3I CTNAG 5 cut(s) 1103, 1367, 1496, 1562, 2105
HpySE526I ACGT 3 cut(s) 474, 618, 1200
Hsp92I GRCGYC 2 cut(s) 474, 1469
Hsp92II CATG 8 cut(s) 145, 172, 190, 401, 715, 1047, 1263, 1467
HspAI GCGC 4 cut(s) 570, 854, 883, 1469
KasI GGCGCC 1 cut(s) 1468
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
LmnI GCTCC 4 cut(s) 257, 677, 947, 995
LweI GCATC 2 cut(s) 277, 1947
MabI ACCWGGT 1 cut(s) 1309
MaeI CTAG 7 cut(s) 449, 819, 1208, 1235, 1389, 1691, 2169
MaeII ACGT 3 cut(s) 474, 618, 1200
MaeIII GTNAC 6 cut(s) 176, 933, 976, 1025, 1196, 1592
MalI GATC 6 cut(s) 8, 102, 122, 1149, 1346, 1749
MboI GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
MboII GAAGA 4 cut(s) 16, 377, 934, 1029
MfeI CAATTG 2 cut(s) 489, 1476
MflI RGATCY 2 cut(s) 1344, 1747
MhlI GDGCHC 5 cut(s) 254, 327, 604, 805, 993
MlsI TGGCCA 1 cut(s) 1706
MluNI TGGCCA 1 cut(s) 1706
Mly113I GGCGCC 1 cut(s) 1469
MlyI GAGTC 3 cut(s) 571, 628, 2132
MmeI TCCRAC 3 cut(s) 109, 635, 949
Mox20I TGGCCA 1 cut(s) 1706
MscI TGGCCA 1 cut(s) 1706
MseI TTAA 6 cut(s) 221, 279, 318, 1134, 1175, 1452
MslI CAYNNNNRTG 3 cut(s) 396, 1042, 1544
Msp20I TGGCCA 1 cut(s) 1706
MspA1I CMGCKG 3 cut(s) 687, 758, 1145
MspI CCGG 8 cut(s) 147, 152, 159, 217, 549, 1014, 1075, 1440
MspR9I CCNGG 6 cut(s) 408, 531, 1076, 1083, 1311, 1530
MssI GTTTAAAC 1 cut(s) 1453
MunI CAATTG 2 cut(s) 489, 1476
Mva1269I GAATGC 3 cut(s) 387, 680, 998
MvaI CCWGG 5 cut(s) 408, 531, 1083, 1311, 1530
MwoI GCNNNNNNNGC 4 cut(s) 296, 669, 1277, 1286
NarI GGCGCC 1 cut(s) 1469
NciI CCSGG 1 cut(s) 1076
NdeI CATATG 1 cut(s) 1942
NdeII GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
NlaIII CATG 8 cut(s) 145, 172, 190, 401, 715, 1047, 1263, 1467
NlaIV GGNNCC 6 cut(s) 658, 706, 1080, 1470, 1735, 1980
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 4 cut(s) 933, 976, 1025, 1196
NspI RCATGY 1 cut(s) 1263
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 168
PaqCI CACCTGC 1 cut(s) 194
PciI ACATGT 1 cut(s) 1259
PctI GAATGC 3 cut(s) 387, 680, 998
PfeI GAWTC 3 cut(s) 453, 464, 1793
PflMI CCANNNNNTGG 3 cut(s) 1853, 1899, 2064
PinAI ACCGGT 1 cut(s) 1439
PleI GAGTC 3 cut(s) 570, 628, 2132
PluTI GGCGCC 1 cut(s) 1472
PmaCI CACGTG 1 cut(s) 1201
PmeI GTTTAAAC 1 cut(s) 1453
PmlI CACGTG 1 cut(s) 1201
PpsI GAGTC 3 cut(s) 570, 628, 2132
Ppu21I YACGTR 1 cut(s) 1201
PpuMI RGGWCCY 3 cut(s) 404, 1079, 1733
PscI ACATGT 1 cut(s) 1259
PsiI TTATAA 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 618
Psp5II RGGWCCY 3 cut(s) 404, 1079, 1733
Psp6I CCWGG 5 cut(s) 406, 529, 1081, 1309, 1528
PspCI CACGTG 1 cut(s) 1201
PspFI CCCAGC 2 cut(s) 586, 1292
PspGI CCWGG 5 cut(s) 406, 529, 1081, 1309, 1528
PspN4I GGNNCC 6 cut(s) 658, 706, 1080, 1470, 1735, 1980
PspPPI RGGWCCY 3 cut(s) 404, 1079, 1733
PstI CTGCAG 2 cut(s) 205, 890
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 2 cut(s) 1344, 1747
PvuII CAGCTG 3 cut(s) 687, 758, 1145
RsaI GTAC 5 cut(s) 91, 210, 1205, 1761, 2099
RsaNI GTAC 5 cut(s) 90, 209, 1204, 1760, 2098
RseI CAYNNNNRTG 3 cut(s) 396, 1042, 1544
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 6 cut(s) 221, 279, 318, 1134, 1175, 1452
Sau3AI GATC 6 cut(s) 6, 100, 120, 1147, 1344, 1747
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 3 cut(s) 571, 628, 2132
ScrFI CCNGG 6 cut(s) 408, 531, 1076, 1083, 1311, 1530
SduI GDGCHC 5 cut(s) 254, 327, 604, 805, 993
SexAI ACCWGGT 1 cut(s) 1309
SfaNI GCATC 2 cut(s) 277, 1947
SfcI CTRYAG 3 cut(s) 201, 886, 1299
SfoI GGCGCC 1 cut(s) 1470
Sfr274I CTCGAG 1 cut(s) 326
SgrAI CRCCGGYG 1 cut(s) 1439
SinI GGWCC 9 cut(s) 149, 260, 404, 775, 1079, 1307, 1649, 1733, 1781
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 396, 1042, 1544
SmlI CTYRAG 2 cut(s) 326, 524
SmoI CTYRAG 2 cut(s) 326, 524
SsiI CCGC 2 cut(s) 1550, 1558
SspDI GGCGCC 1 cut(s) 1468
SspI AATATT 1 cut(s) 316
SspMI CTAG 7 cut(s) 449, 819, 1208, 1235, 1389, 1691, 2169
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 6 cut(s) 406, 529, 1074, 1081, 1309, 1528
StyI CCWWGG 3 cut(s) 256, 263, 1707
TaaI ACNGT 6 cut(s) 52, 606, 765, 911, 1131, 1196
TaiI ACGT 3 cut(s) 477, 621, 1203
TaqI TCGA 2 cut(s) 327, 471
TatI WGTACW 4 cut(s) 89, 208, 1759, 2097
TauI GCSGC 1 cut(s) 1561
TfiI GAWTC 3 cut(s) 453, 464, 1793
Tru1I TTAA 6 cut(s) 221, 279, 318, 1134, 1175, 1452
Tru9I TTAA 6 cut(s) 221, 279, 318, 1134, 1175, 1452
TscAI CASTG 7 cut(s) 84, 205, 742, 1012, 1383, 2164, 2187
TseFI GTSAC 4 cut(s) 933, 976, 1025, 1196
Tsp45I GTSAC 4 cut(s) 933, 976, 1025, 1196
TspDTI ATGAA 8 cut(s) 17, 226, 445, 1110, 1629, 1841, 1848, 2109
TspGWI ACGGA 3 cut(s) 717, 1232, 1996
TspRI CASTG 7 cut(s) 84, 205, 742, 1012, 1383, 2164, 2187
Van91I CCANNNNNTGG 3 cut(s) 1853, 1899, 2064
VpaK11BI GGWCC 9 cut(s) 149, 260, 404, 775, 1079, 1307, 1649, 1733, 1781
XapI RAATTY 5 cut(s) 17, 1535, 1607, 1679, 2086
XceI RCATGY 1 cut(s) 1263
XcmI CCANNNNNNNNNTGG 3 cut(s) 404, 671, 965
XhoI CTCGAG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 7 cut(s) 449, 819, 1208, 1235, 1389, 1691, 2169
ZraI GACGTC 1 cut(s) 475
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.