Rh6DG440400

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0017388
Physical Location & Seq
Reverse (-)
61661726 .. 61665093
3368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG440400.1

Sequence Viewer

Length: 2145 bp
ATGACCGGACCGGCAGCCGGAGTGGTCATGATGGGTTACGCCAACATGGGCCAAACCACTGCGGGTGTACATTTCCGGCTAAGGGCAAGGACTTTCATCGTGGCCGAGAGCTCCCAAGGTCCAAGGTTTGCCTTTGTTAATCTTGATGCAGGGATGGCTTCACAACTCGTTAATATTAAAGTGCTCGAGAAACTCAAATCAAGGTTTGGCGATTTGTATACCGAAGAAAATGTGGCGATTAGTGGCATTCACACCCATGCAGGACCTGGAGGTTATTTGCAGTATTTTCTTTACTCTATATCAACATTAGGATTCATCCAAGAATCATTCGATGTCATTGTCAACGCAATTGTGCAAAGCATTGTTCAGGCTCATCATAACCTCAAGCCTGGTTCTATTTTCATCAACCAAGGGGATGTGGTAAACGCCGGAATAAACAGGAGTCCAAGCGCTTATTTGCTAAACCCAGCAGAGGAGAGAGCACAGTATCCGACCAACGTTGATACCCTAATGACTCTTTTGAAGTTTGTGGATGGTGCCAGCCAACAGAGCATTGGAGCATTCAGCTGGTTTGCCACTCACGGAACCTCCATGAGCAAAGAGAATAAGCTCATCAGTGGAGACAACAAAGGTGCAGCTGCACGGTTCTTCGAGGACCAGTTTGCTACAACTCCCATCAGAGCACCCTTCAACTCCTCTAGCAAACCAGATATTGGTGAGTTAGTAAAGAAGGCGCAGACAATCAAAGCAACAGGAGGGCAGCGCTGCAGTAAAACGAGCAGCCAAGACTGCAAGGTGAGGAAGAACGACGGGTCACTATTCGTGGGAGCTTTTTGCCAATCTAATGTTGGAGATGTGACCCCGAATGTGCTCGGAGCATTCTGCACTGATACCGGAAGACCTTGTGACTTCAATACATCCTCATGTAATGGGAACGACCTGCTTTGCCTCGGCCAGGGACCTGGGTATCCTGATGAAATACTAAGCACCAAAATTATAGGGGAAAGACAGTTTAAGAAGGCAGCTGATCTATTTACATCGGCTTCAGAGAAATTAACCGGGGCTATTGATTACCGTCACGTGTACCTAGACTTTACGGATATTGAGGTTGAGCTAGAAGGAAACAAGAAGGTGAAAACATGTCCAGCGGCAGTTGGTGCTGGTTTTGCTGCTGGGACTACAGATGGACCTGGTGTCTCTGGGTTTCAACAAGGTGATACAGAGATCCCTGAAAAATATAGAAAACTTAGGGACGCACTGCAAAAACCTAGCGAATATCAAGTGGACTGCCAAGAACCCAAGAATGTTCTGCTTTCCACCGGTGAAATGTTTAAACCTTACGCATGGGCGCCTGCAATTGTCCCTATACAAATGCTCAGATTGGGAAAACTCATCATACTTTCTGTCCCAGGAGAATTCACCACAATGGCGGGGAGGCGGCTAAGGGCCGCAGTAAAGGAGACATTGATAAGTAACAGCAAGGGTGAATTTGATGAAAACACCCACATAGTAATAGCAGGCCTTACAAATACTTATTCCCAATATATAGCAACTTTCGAAGAGTACACACAACAGAGATATGAGGCTGCTTCCACTCTCTATGGTCCTCACACATTATCAGCATACATCCAAGAATTTGAGAAATTAGCCAAAGCAATGGCCAAGGGAGAAAAGGTCATTTCTAAAGGTCCCTCGCCACCAGATCTTTCCTCTGTACAAGTCGAAGTCTTACTTGGACCTTTTGGAGATTCGCCTCCACCACATACAAAGTTTGGAGATATGAAACAAGATATCATTATACCAAAAAGTGGATCATTCAGAAAGGGAGATTTGAATAGACCAAATGCCACATTTTGGAGTGCAAACCCAAACTATGACTTGTTGACAGAAGGCACATATGCTGTGGTAGAGATGCTTCAAGGGGATAACTGGGTTCCAGTTTACGATGATGATGATTTCTCCGTGGTTTTCAAGTGGAATAGAGATGACAAGGGATTATATAGTGCAGCAACCATAGAATGGGAAATACCAATTTATGCAAATTTGGGGGTGTACAGGCTCAGGCATTTTGGTTCATCAAGGAAAAAGAAGGACTCCCCCATCACTTACTTCACTGGTGCATCTAGTGGGTTTGCAGTGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

714

Amino Acids

77.84

Weight (kDa)

6.31

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 1 - 544 3.1e-208 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 546 - 713 4.7e-42 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017388)

Species Orthologous Gene IDs
malus_domestica MD08G1004600.v1.1
prunus_persica Prupe.1G356500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304021
rosa_laevigata RLG00000011024
rosa_multiflora Rmu_sc0003805.1_g000016
rosa_roxburghii Rroxscaffold_7G00164170
rosa_rugosa Rorug06G0327200 Rorug06G0327200
rosa_samantha Rh6AG440900 Rh6BG461900 Rh6CG453100 Rh6DG440400
rosa_wichuraiana Rw6G038200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 948
AccB1I GGYRCC 2 cut(s) 536, 1348
AccB7I CCANNNNNTGG 4 cut(s) 713, 1808, 1854, 2019
AccI GTMKAC 1 cut(s) 218
AciI CCGC 5 cut(s) 62, 1148, 1430, 1438, 1449
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 1219, 1819
AcoI YGGCCR 3 cut(s) 102, 952, 1659
AcsI RAATTY 4 cut(s) 1415, 1487, 1634, 2041
AcuI CTGAAG 1 cut(s) 1029
AcvI CACGTG 1 cut(s) 1081
AcyI GRCGYC 1 cut(s) 1349
AfaI GTAC 5 cut(s) 69, 1085, 1565, 1716, 2054
AfeI AGCGCT 2 cut(s) 451, 764
AfiI CCNNNNNNNGG 8 cut(s) 17, 82, 472, 713, 955, 1808, 1854, 2019
AflIII ACRYGT 2 cut(s) 1080, 1139
AgeI ACCGGT 1 cut(s) 1319
AgsI TTSAA 7 cut(s) 523, 691, 913, 1208, 1834, 1919, 1972
AhdI GACNNNNNGTC 1 cut(s) 1193
AjnI CCWGG 6 cut(s) 265, 388, 954, 961, 1189, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 1716, 1748
AluBI AGCT 7 cut(s) 111, 567, 610, 638, 830, 1025, 1114
AluI AGCT 7 cut(s) 111, 567, 610, 638, 830, 1025, 1114
Alw21I GWGCWC 5 cut(s) 113, 186, 484, 685, 873
Alw26I GTCTC 3 cut(s) 615, 1201, 1454
AlwI GGATC 2 cut(s) 1219, 1819
AlwNI CAGNNNCTG 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 185
Aor51HI AGCGCT 2 cut(s) 451, 764
AoxI GGCC 6 cut(s) 49, 102, 952, 1446, 1519, 1659
ApoI RAATTY 4 cut(s) 1415, 1487, 1634, 2041
AsiGI ACCGGT 1 cut(s) 1319
AspLEI GCGC 4 cut(s) 452, 736, 765, 1351
AsuC2I CCSGG 1 cut(s) 1060
AsuHPI GGTGA 7 cut(s) 728, 808, 1144, 1226, 1334, 1411, 1496
AsuII TTCGAA 1 cut(s) 1557
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 9 cut(s) 8, 119, 263, 655, 959, 1187, 1604, 1688, 1736
BaeI ACNNNNGTAYC 2 cut(s) 950, 983
BalI TGGCCA 1 cut(s) 1661
BanI GGYRCC 2 cut(s) 536, 1348
BanII GRGCYC 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 1899, 1931
BbrPI CACGTG 1 cut(s) 1081
BbsI GAAGAC 1 cut(s) 904
Bbv12I GWGCWC 5 cut(s) 113, 186, 484, 685, 873
BccI CCATC 6 cut(s) 25, 148, 527, 683, 1178, 2108
BciT130I CCWGG 6 cut(s) 267, 390, 956, 963, 1191, 1410
BciVI GTATCC 2 cut(s) 498, 978
BcnI CCSGG 1 cut(s) 1060
BcoDI GTCTC 3 cut(s) 615, 1201, 1454
BfaI CTAG 5 cut(s) 699, 1088, 1115, 1269, 2124
BfmI CTRYAG 2 cut(s) 766, 1179
BfoI RGCGCY 3 cut(s) 453, 766, 1352
BfuAI ACCTGC 1 cut(s) 948
BfuI GTATCC 2 cut(s) 498, 978
BglII AGATCT 1 cut(s) 1702
Bme1390I CCNGG 7 cut(s) 267, 390, 956, 963, 1060, 1191, 1410
Bme18I GGWCC 9 cut(s) 8, 119, 263, 655, 959, 1187, 1604, 1688, 1736
BmeRI GACNNNNNGTC 1 cut(s) 1193
BmeT110I CYCGRG 1 cut(s) 185
BmiI GGNNCC 6 cut(s) 538, 586, 960, 1350, 1690, 1935
BmrFI CCNGG 7 cut(s) 267, 390, 956, 963, 1060, 1191, 1410
BmrI ACTGGG 1 cut(s) 1939
BmsI GCATC 3 cut(s) 136, 1902, 2129
BmuI ACTGGG 1 cut(s) 1939
BpiI GAAGAC 1 cut(s) 904
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 3 cut(s) 80, 1442, 2060
Bpu14I TTCGAA 1 cut(s) 1557
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 1060
BsaAI YACGTR 1 cut(s) 1081
BsaHI GRCGYC 1 cut(s) 1349
BsaWI WCCGGW 3 cut(s) 5, 893, 1319
BsaXI ACNNNNNCTCC 4 cut(s) 572, 602, 1659, 1689
Bsc4I CCNNNNNNNGG 8 cut(s) 17, 82, 472, 713, 955, 1808, 1854, 2019
Bse118I RCCGGY 2 cut(s) 10, 1319
Bse1I ACTGG 4 cut(s) 658, 1934, 1937, 2119
Bse3DI GCAATG 1 cut(s) 1662
BseBI CCWGG 6 cut(s) 267, 390, 956, 963, 1191, 1410
BseGI GGATG 6 cut(s) 159, 315, 421, 538, 917, 1626
BseLI CCNNNNNNNGG 8 cut(s) 17, 82, 472, 713, 955, 1808, 1854, 2019
BseMI GCAATG 1 cut(s) 1662
BseMII CTCAG 2 cut(s) 1390, 2074
BseNI ACTGG 4 cut(s) 658, 1934, 1937, 2119
BseRI GAGGAG 2 cut(s) 488, 685
BseYI CCCAGC 2 cut(s) 466, 1172
BsgI GTGCAG 4 cut(s) 624, 654, 868, 2025
BshFI GGCC 6 cut(s) 51, 104, 954, 1448, 1521, 1661
BshNI GGYRCC 2 cut(s) 536, 1348
BshTI ACCGGT 1 cut(s) 1319
BsiHKAI GWGCWC 5 cut(s) 113, 186, 484, 685, 873
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 8 cut(s) 6, 11, 18, 76, 429, 894, 1059, 1320
BslFI GGGAC 6 cut(s) 972, 1189, 1265, 1346, 1391, 1674
BslI CCNNNNNNNGG 8 cut(s) 17, 82, 472, 713, 955, 1808, 1854, 2019
BsmAI GTCTC 3 cut(s) 615, 1201, 1454
BsmFI GGGAC 6 cut(s) 972, 1189, 1265, 1346, 1391, 1674
BsmI GAATGC 3 cut(s) 246, 560, 878
BsnI GGCC 6 cut(s) 51, 104, 954, 1448, 1521, 1661
BsoBI CYCGRG 1 cut(s) 185
Bsp119I TTCGAA 1 cut(s) 1557
Bsp1286I GDGCHC 5 cut(s) 113, 186, 484, 685, 873
Bsp1407I TGTACA 3 cut(s) 67, 1714, 2052
Bsp143I GATC 4 cut(s) 1027, 1224, 1702, 1811
BspACI CCGC 5 cut(s) 62, 1148, 1430, 1438, 1449
BspANI GGCC 6 cut(s) 51, 104, 954, 1448, 1521, 1661
BspCNI CTCAG 2 cut(s) 1389, 2073
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 6 cut(s) 538, 586, 960, 1350, 1690, 1935
BspMAI CTGCAG 1 cut(s) 770
BspMI ACCTGC 1 cut(s) 948
BspPI GGATC 2 cut(s) 1219, 1819
BspT104I TTCGAA 1 cut(s) 1557
BspT107I GGYRCC 2 cut(s) 536, 1348
BsrDI GCAATG 1 cut(s) 1662
BsrFI RCCGGY 2 cut(s) 10, 1319
BsrGI TGTACA 3 cut(s) 67, 1714, 2052
BsrI ACTGG 4 cut(s) 658, 1934, 1937, 2119
BssAI RCCGGY 2 cut(s) 10, 1319
BssMI GATC 4 cut(s) 1027, 1224, 1702, 1811
BssNAI GTATAC 1 cut(s) 219
BssNI GRCGYC 1 cut(s) 1349
BssT1I CCWWGG 4 cut(s) 115, 122, 409, 1662
Bst1107I GTATAC 1 cut(s) 219
Bst2UI CCWGG 6 cut(s) 267, 390, 956, 963, 1191, 1410
Bst4CI ACNGT 4 cut(s) 486, 645, 1011, 1076
Bst6I CTCTTC 1 cut(s) 1554
BstACI GRCGYC 1 cut(s) 1349
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstAUI TGTACA 3 cut(s) 67, 1714, 2052
BstBAI YACGTR 1 cut(s) 1081
BstBI TTCGAA 1 cut(s) 1557
BstC8I GCNNGC 3 cut(s) 541, 1353, 1519
BstDEI CTNAG 6 cut(s) 80, 983, 1247, 1376, 1442, 2060
BstDSI CCRYGG 1 cut(s) 1962
BstENI CCTNNNNNAGG 1 cut(s) 953
BstF5I GGATG 6 cut(s) 159, 315, 421, 538, 917, 1626
BstH2I RGCGCY 3 cut(s) 453, 766, 1352
BstHHI GCGC 4 cut(s) 452, 736, 765, 1351
BstKTI GATC 4 cut(s) 1030, 1227, 1705, 1814
BstMAI GTCTC 3 cut(s) 615, 1201, 1454
BstMBI GATC 4 cut(s) 1027, 1224, 1702, 1811
BstMWI GCNNNNNNNGC 5 cut(s) 155, 549, 789, 1157, 1166
BstNI CCWGG 6 cut(s) 267, 390, 956, 963, 1191, 1410
BstNSI RCATGY 1 cut(s) 1143
BstSCI CCNGG 7 cut(s) 265, 388, 954, 961, 1058, 1189, 1408
BstSFI CTRYAG 2 cut(s) 766, 1179
BstV2I GAAGAC 1 cut(s) 904
BstX2I RGATCY 2 cut(s) 1224, 1702
BstXI CCANNNNNNTGG 2 cut(s) 962, 1657
BstYI RGATCY 2 cut(s) 1224, 1702
BstZ17I GTATAC 1 cut(s) 219
BsuI GTATCC 2 cut(s) 498, 978
BsuRI GGCC 6 cut(s) 51, 104, 954, 1448, 1521, 1661
BtgI CCRYGG 1 cut(s) 1962
BtsCI GGATG 6 cut(s) 159, 315, 421, 538, 917, 1626
BtsI GCAGTG 3 cut(s) 57, 1256, 2142
BtsIMutI CAGTG 6 cut(s) 57, 622, 885, 1256, 2112, 2142
BveI ACCTGC 1 cut(s) 948
Cac8I GCNNGC 3 cut(s) 541, 1353, 1519
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 27
CfoI GCGC 4 cut(s) 452, 736, 765, 1351
Cfr10I RCCGGY 2 cut(s) 10, 1319
CpoI CGGWCCG 1 cut(s) 8
CseI GACGC 1 cut(s) 1262
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 5 cut(s) 68, 1084, 1564, 1715, 2053
CspAI ACCGGT 1 cut(s) 1319
CspI CGGWCCG 1 cut(s) 8
CviAII CATG 7 cut(s) 28, 46, 257, 592, 924, 1140, 1344
CviQI GTAC 5 cut(s) 68, 1084, 1564, 1715, 2053
DdeI CTNAG 6 cut(s) 80, 983, 1247, 1376, 1442, 2060
DinI GGCGCC 1 cut(s) 1350
DpnI GATC 4 cut(s) 1029, 1226, 1704, 1813
DpnII GATC 4 cut(s) 1027, 1224, 1702, 1811
DraI TTTAAA 1 cut(s) 1333
DriI GACNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 3 cut(s) 102, 952, 1659
Eam1104I CTCTTC 1 cut(s) 1554
Eam1105I GACNNNNNGTC 1 cut(s) 1193
EarI CTCTTC 1 cut(s) 1554
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 4 cut(s) 115, 122, 409, 1662
Eco147I AGGCCT 1 cut(s) 1521
Eco24I GRGCYC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 1792
Eco47I GGWCC 9 cut(s) 8, 119, 263, 655, 959, 1187, 1604, 1688, 1736
Eco47III AGCGCT 2 cut(s) 451, 764
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 1029
Eco72I CACGTG 1 cut(s) 1081
Eco88I CYCGRG 1 cut(s) 185
EcoICRI GAGCTC 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 953
EcoO109I RGGNCCY 3 cut(s) 263, 959, 1688
EcoRI GAATTC 1 cut(s) 1415
EcoRII CCWGG 6 cut(s) 265, 388, 954, 961, 1189, 1408
EcoRV GATATC 1 cut(s) 1792
EcoT14I CCWWGG 4 cut(s) 115, 122, 409, 1662
EcoT38I GRGCYC 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 1350
EheI GGCGCC 1 cut(s) 1350
ErhI CCWWGG 4 cut(s) 115, 122, 409, 1662
FaeI CATG 7 cut(s) 31, 49, 260, 595, 927, 1143, 1347
FalI AAGNNNNNCTT 2 cut(s) 1716, 1748
FaqI GGGAC 6 cut(s) 972, 1189, 1265, 1346, 1391, 1674
FatI CATG 7 cut(s) 27, 45, 256, 591, 923, 1139, 1343
FauI CCCGC 2 cut(s) 55, 1423
FauNDI CATATG 1 cut(s) 1897
FblI GTMKAC 1 cut(s) 218
FokI GGATG 6 cut(s) 166, 302, 428, 545, 904, 1613
FriOI GRGCYC 1 cut(s) 113
FspBI CTAG 5 cut(s) 699, 1088, 1115, 1269, 2124
GlaI GCGC 4 cut(s) 451, 735, 764, 1350
GsaI CCCAGC 2 cut(s) 470, 1176
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 3 cut(s) 453, 766, 1352
HaeIII GGCC 6 cut(s) 51, 104, 954, 1448, 1521, 1661
HapII CCGG 8 cut(s) 6, 11, 18, 76, 429, 894, 1059, 1320
HgaI GACGC 1 cut(s) 1262
HhaI GCGC 4 cut(s) 452, 736, 765, 1351
Hin1I GRCGYC 1 cut(s) 1349
Hin1II CATG 7 cut(s) 31, 49, 260, 595, 927, 1143, 1347
Hin6I GCGC 4 cut(s) 450, 734, 763, 1349
HinP1I GCGC 4 cut(s) 450, 734, 763, 1349
HincII GTYRAC 2 cut(s) 343, 1884
HindII GTYRAC 2 cut(s) 343, 1884
HinfI GANTC 6 cut(s) 312, 323, 442, 514, 1748, 2093
HpaII CCGG 8 cut(s) 6, 11, 18, 76, 429, 894, 1059, 1320
HphI GGTGA 7 cut(s) 728, 808, 1144, 1226, 1334, 1411, 1496
Hpy188I TCNGA 6 cut(s) 492, 680, 875, 1048, 1379, 1820
Hpy188III TCNNGA 4 cut(s) 28, 143, 187, 971
Hpy99I CGWCG 1 cut(s) 812
HpyAV CCTTC 7 cut(s) 697, 724, 1012, 1112, 1123, 1883, 2083
HpyCH4III ACNGT 4 cut(s) 486, 645, 1011, 1076
HpyCH4IV ACGT 2 cut(s) 498, 1080
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 549, 789, 1157, 1166
HpyF3I CTNAG 6 cut(s) 80, 983, 1247, 1376, 1442, 2060
HpySE526I ACGT 2 cut(s) 498, 1080
Hsp92I GRCGYC 1 cut(s) 1349
Hsp92II CATG 7 cut(s) 31, 49, 260, 595, 927, 1143, 1347
HspAI GCGC 4 cut(s) 450, 734, 763, 1349
KasI GGCGCC 1 cut(s) 1348
Kzo9I GATC 4 cut(s) 1027, 1224, 1702, 1811
LmnI GCTCC 4 cut(s) 116, 557, 827, 875
LweI GCATC 3 cut(s) 136, 1902, 2129
MabI ACCWGGT 1 cut(s) 1189
MaeI CTAG 5 cut(s) 699, 1088, 1115, 1269, 2124
MaeII ACGT 2 cut(s) 498, 1080
MaeIII GTNAC 6 cut(s) 35, 813, 856, 905, 1076, 1472
MalI GATC 4 cut(s) 1029, 1226, 1704, 1813
MboI GATC 4 cut(s) 1027, 1224, 1702, 1811
MboII GAAGA 5 cut(s) 236, 640, 814, 909, 1571
MfeI CAATTG 2 cut(s) 348, 1356
MflI RGATCY 2 cut(s) 1224, 1702
MhlI GDGCHC 5 cut(s) 113, 186, 484, 685, 873
MlsI TGGCCA 1 cut(s) 1661
MluNI TGGCCA 1 cut(s) 1661
Mly113I GGCGCC 1 cut(s) 1349
MlyI GAGTC 3 cut(s) 451, 508, 2087
MmeI TCCRAC 2 cut(s) 515, 829
Mox20I TGGCCA 1 cut(s) 1661
MscI TGGCCA 1 cut(s) 1661
MseI TTAA 6 cut(s) 138, 171, 177, 1014, 1055, 1332
MslI CAYNNNNRTG 3 cut(s) 255, 922, 1424
Msp20I TGGCCA 1 cut(s) 1661
MspA1I CMGCKG 4 cut(s) 567, 638, 1025, 1148
MspI CCGG 8 cut(s) 6, 11, 18, 76, 429, 894, 1059, 1320
MspR9I CCNGG 7 cut(s) 267, 390, 956, 963, 1060, 1191, 1410
MssI GTTTAAAC 1 cut(s) 1333
MunI CAATTG 2 cut(s) 348, 1356
Mva1269I GAATGC 3 cut(s) 246, 560, 878
MvaI CCWGG 6 cut(s) 267, 390, 956, 963, 1191, 1410
MwoI GCNNNNNNNGC 5 cut(s) 155, 549, 789, 1157, 1166
NarI GGCGCC 1 cut(s) 1349
NciI CCSGG 1 cut(s) 1060
NdeI CATATG 1 cut(s) 1897
NdeII GATC 4 cut(s) 1027, 1224, 1702, 1811
NlaIII CATG 7 cut(s) 31, 49, 260, 595, 927, 1143, 1347
NlaIV GGNNCC 6 cut(s) 538, 586, 960, 1350, 1690, 1935
NmeAIII GCCGAG 2 cut(s) 130, 930
NmuCI GTSAC 4 cut(s) 813, 856, 905, 1076
NspI RCATGY 1 cut(s) 1143
NspV TTCGAA 1 cut(s) 1557
PaeR7I CTCGAG 1 cut(s) 185
PagI TCATGA 1 cut(s) 27
PceI AGGCCT 1 cut(s) 1521
PciI ACATGT 1 cut(s) 1139
PctI GAATGC 3 cut(s) 246, 560, 878
PfeI GAWTC 3 cut(s) 312, 323, 1748
PflMI CCANNNNNTGG 4 cut(s) 713, 1808, 1854, 2019
PinAI ACCGGT 1 cut(s) 1319
PleI GAGTC 3 cut(s) 450, 508, 2087
PluTI GGCGCC 1 cut(s) 1352
PmaCI CACGTG 1 cut(s) 1081
PmeI GTTTAAAC 1 cut(s) 1333
PmlI CACGTG 1 cut(s) 1081
PpsI GAGTC 3 cut(s) 450, 508, 2087
Ppu21I YACGTR 1 cut(s) 1081
PpuMI RGGWCCY 3 cut(s) 263, 959, 1688
PscI ACATGT 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 113
Psp1406I AACGTT 1 cut(s) 498
Psp5II RGGWCCY 3 cut(s) 263, 959, 1688
Psp6I CCWGG 6 cut(s) 265, 388, 954, 961, 1189, 1408
PspCI CACGTG 1 cut(s) 1081
PspFI CCCAGC 2 cut(s) 466, 1172
PspGI CCWGG 6 cut(s) 265, 388, 954, 961, 1189, 1408
PspN4I GGNNCC 6 cut(s) 538, 586, 960, 1350, 1690, 1935
PspPPI RGGWCCY 3 cut(s) 263, 959, 1688
PstI CTGCAG 1 cut(s) 770
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 2 cut(s) 1224, 1702
PvuII CAGCTG 3 cut(s) 567, 638, 1025
RsaI GTAC 5 cut(s) 69, 1085, 1565, 1716, 2054
RsaNI GTAC 5 cut(s) 68, 1084, 1564, 1715, 2053
RseI CAYNNNNRTG 3 cut(s) 255, 922, 1424
Rsr2I CGGWCCG 1 cut(s) 8
RsrII CGGWCCG 1 cut(s) 8
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 6 cut(s) 138, 171, 177, 1014, 1055, 1332
Sau3AI GATC 4 cut(s) 1027, 1224, 1702, 1811
SchI GAGTC 3 cut(s) 451, 508, 2087
ScrFI CCNGG 7 cut(s) 267, 390, 956, 963, 1060, 1191, 1410
SduI GDGCHC 5 cut(s) 113, 186, 484, 685, 873
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 3 cut(s) 136, 1902, 2129
SfcI CTRYAG 2 cut(s) 766, 1179
SfoI GGCGCC 1 cut(s) 1350
Sfr274I CTCGAG 1 cut(s) 185
SfuI TTCGAA 1 cut(s) 1557
SgrAI CRCCGGYG 1 cut(s) 1319
SinI GGWCC 9 cut(s) 8, 119, 263, 655, 959, 1187, 1604, 1688, 1736
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 3 cut(s) 255, 922, 1424
SmlI CTYRAG 2 cut(s) 185, 383
SmoI CTYRAG 2 cut(s) 185, 383
SseBI AGGCCT 1 cut(s) 1521
SsiI CCGC 5 cut(s) 62, 1148, 1430, 1438, 1449
SspDI GGCGCC 1 cut(s) 1348
SspI AATATT 1 cut(s) 175
SspMI CTAG 5 cut(s) 699, 1088, 1115, 1269, 2124
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 1 cut(s) 1521
StyD4I CCNGG 7 cut(s) 265, 388, 954, 961, 1058, 1189, 1408
StyI CCWWGG 4 cut(s) 115, 122, 409, 1662
TaaI ACNGT 4 cut(s) 486, 645, 1011, 1076
TaiI ACGT 2 cut(s) 501, 1083
TaqI TCGA 5 cut(s) 186, 330, 651, 1557, 1722
TatI WGTACW 4 cut(s) 67, 1563, 1714, 2052
TauI GCSGC 3 cut(s) 1151, 1441, 1451
TfiI GAWTC 3 cut(s) 312, 323, 1748
Tru1I TTAA 6 cut(s) 138, 171, 177, 1014, 1055, 1332
Tru9I TTAA 6 cut(s) 138, 171, 177, 1014, 1055, 1332
TscAI CASTG 6 cut(s) 64, 622, 892, 1263, 2119, 2142
TseFI GTSAC 4 cut(s) 813, 856, 905, 1076
Tsp45I GTSAC 4 cut(s) 813, 856, 905, 1076
TspDTI ATGAA 7 cut(s) 85, 304, 391, 990, 1509, 1796, 2064
TspGWI ACGGA 3 cut(s) 597, 1112, 1951
TspRI CASTG 6 cut(s) 64, 622, 892, 1263, 2119, 2142
Van91I CCANNNNNTGG 4 cut(s) 713, 1808, 1854, 2019
VpaK11BI GGWCC 9 cut(s) 8, 119, 263, 655, 959, 1187, 1604, 1688, 1736
XagI CCTNNNNNAGG 1 cut(s) 953
XapI RAATTY 4 cut(s) 1415, 1487, 1634, 2041
XceI RCATGY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 3 cut(s) 263, 551, 845
XhoI CTCGAG 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 218
XspI CTAG 5 cut(s) 699, 1088, 1115, 1269, 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.