Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007051

Physical Location & Seq

Genomic Coordinates Forward (+)

1936401 .. 1940798

Gene Structure 4398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1026500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGTCCGGACCTCTCGATCGTTTCGCAAGGCCTTGTTTTGAGGGGTTCTCTAGCCACGACGAAAGGAGGGAAAGGAAATCGGATTTTGAAAACTCGGAGGAGGAGAGGAGGACTGGAATTGGTGGGAATTTGAAGAAGAAAGCAATCAATGCGTCCACTAAATTCAGACGTTCTCTCAAGAAGAAGAGGAGGAAGAGTGGTGGTGGTGGTGGTAGTAGGCTGTCCACTTCAGTTTCGATTGAGGATGTAAGGGACGCCAAGGAGCTACGGGCTGTTGACGCTTTTCGGCAGGTGCTTATTTCGGAAGACTTGCTGCCTTCAAGACATGATGACTATCATATGTTATTGAGATTTTTGATAGCAAGGAAGTTCGATATTGAGAAAGCAAAGCACATGTGGGCCAATATGATTCAATGGAGGAAAGATTTTGGTACTGATACAATTTTGGAGGATTTTGAGTTCAGTGAGTTGAATGAAGTTCGAAAGCACTACCCTCAGTCTTATCATGGTGTTGACAAGGAGGGAAGACCAGTTTACATTGAAAGGTTGGGGAAAGTTGATCCCACCAAGCTTATGCAAGTGACTTCCTTGGAACGCTATGTGAGATACCATGTGCAGGAGTATGAAAGGTGTTTTGCCATTAAGTTTCCTGCCTGCTCTATTGCTGCAAAGAGACATATAGATTCAAGCACAACAATTATAGATGTTCAAGGCGTGGGTCTTAAAAACTTTACCAAGTCTGCACGAGATCTCACAGAGCAGCTGCAGAAGATTGATAGTGACAACTATCCTGAGACGCTTTGCCGAATGTTTATAATCAATGCTAGTTATGCTTTTAGGAAGGTGCTCTGGCCTACAGTTAGAGCGTTCCTCGATGCCAGAACAACCAGCAAAATTCATGTTTTGAGTAACAAGTACCAAAGCAACTTACTTGAAATAATCGATGCAAGTGAGTTGCCAGAGTTTTTGGGTGGTAGCTGTACCTGTTCCGATCAGGGAGGTTGTATGTCGTCTGATAAAGGGCCATGGAAGGACCCAAACATACTGAAGATGGTAATTAGTGGCGAAGCACAATGTTTCAGACAAATAGTTACAGTTTCAAACAGTGAAGGGAGGATAAGTGTTTCTGATAAGCCACGCAATCCATTGATAAGAAGTAGTGATACATCTACTGCAGAGTCTGGATCTGAAGTGGAAGAAATGGGTTCACCTAAACCAACGAGGAACTACTTACAACCTAAGTTGACTCCTGTTTGTGAAGAAGCTAGGGTAGTCGGTAAAGCGAGCATTGCTACTGGCTTGCCGGAGTACGATGAACTTGTTCCCATGGTTGACAAGGCTGTGGATATGGGATGGAAGAAACCAGAATTACAACCACCATTTGCTTCTAGAGGGGCATCTTCTCCAAGATCTGTGGGGAAAACAACAGATGCCATGCATGCTAATATCTGGACTATGCTTGTGGCATTCTTTGTAACCCTCATTTCACTAATCCGATCTTTTTCACTGGGGGCGACTAAGAAACTTAAAAACTCTGTTTCTGATTCTGCCCACAACATTCCTGACCTAACCGATGAGTCAACTGCCGTGGAGGAATTCAGGCCCCCATCACCCGTACCGGGGTTATCCAGGGAAAACCTTCTCTCATCTGCTTTGAAGAAGCTTGGTGAACTTGAGGAGAAGGTAGATATGCTGCAAGCAAAGCCTCTTGAGATGCCTTACGAGAAAGTGGAGCTACTGAATGCTGCTGTTTATCGTGTAGATGCGTTAGAATCAGAGTTAATTGCAACAAAAAAGGCTCTGCATGAGGCTTTAATGAGGCAGGAAGAACTTCTTGCCTATATAGACAGGCAAGAACAGGCCAAGCTTCGGAAAAAAAGATGTTGCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008150 GO:0008525 GO:0008526 GO:0009653 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044424 GO:0044464 GO:0044703 GO:0048193 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0071702 GO:0071840 GO:0071944 GO:0120009 GO:0120010 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

631

Amino Acids

71.24

Weight (kDa)

8.72

Isoelectric Point (pI)

52.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	109 - 134	1e-05	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	162 - 325	1.8e-29	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19650 AT1G19650 AT1G19650 AT1G75370 AT1G75370
fragaria_vesca	FvH4_2g39420 FvH4_2g39420
malus_domestica	MD08G1026500.v1.1 MD15G1023300.v1.1
prunus_persica	Prupe.1G375200_v2.0.a1 Prupe.1G375200_v2.0.a1
pyrus_communis	pycom08g02050 pycom15g01930
rosa_chinensis	RchiOBHm_Chr6g0301561
rosa_laevigata	RLG00000011244
rosa_multiflora	Rmu_ssc0000452.1_g000004
rosa_roxburghii	Rroxscaffold_7G00166650
rosa_rugosa	Rorug06G0306100 Rorug06G0306200 Rorug06G0306300
rosa_samantha	Rh6AG419800 Rh6BG425800 Rh6CG432800 Rh6DG419000
rosa_wichuraiana	Rw6G036290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	815
AarI	CACCTGC	1 cut(s)	280
Acc36I	ACCTGC	1 cut(s)	280
AccIII	TCCGGA	1 cut(s)	5
AclWI	GGATC	2 cut(s)	554, 1192
AcsI	RAATTY	4 cut(s)	127, 161, 894, 1595
AcuI	CTGAAG	3 cut(s)	213, 1067, 1209
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	5 cut(s)	433, 917, 982, 1310, 1617
AfiI	CCNNNNNNNGG	4 cut(s)	616, 1619, 1620, 1869
AflIII	ACRYGT	1 cut(s)	393
AjnI	CCWGG	1 cut(s)	1628
AloI	GAACNNNNNNTCC	2 cut(s)	443, 475
AluBI	AGCT	8 cut(s)	265, 571, 763, 978, 1265, 1663, 1735, 1867
AluI	AGCT	8 cut(s)	265, 571, 763, 978, 1265, 1663, 1735, 1867
Alw21I	GWGCWC	1 cut(s)	849
Alw26I	GTCTC	2 cut(s)	667, 788
AlwI	GGATC	2 cut(s)	554, 1192
AlwNI	CAGNNNCTG	1 cut(s)	1181
Aor13HI	TCCGGA	1 cut(s)	5
AoxI	GGCC	6 cut(s)	29, 399, 851, 1022, 1601, 1860
ApeKI	GCWGC	6 cut(s)	313, 665, 760, 763, 1693, 1745
ApoI	RAATTY	4 cut(s)	127, 161, 894, 1595
Asp700I	GAANNNNTTC	3 cut(s)	1320, 1638, 1830
AspS9I	GGNCC	5 cut(s)	8, 399, 1022, 1033, 1602
AsuC2I	CCSGG	1 cut(s)	1620
AsuHPI	GGTGA	3 cut(s)	1200, 1602, 1679
AsuII	TTCGAA	1 cut(s)	481
AvaII	GGWCC	2 cut(s)	8, 1033
BarI	GAAGNNNNNNTAC	2 cut(s)	1147, 1179
BauI	CACGAG	1 cut(s)	744
BbsI	GAAGAC	2 cut(s)	312, 532
Bbv12I	GWGCWC	1 cut(s)	849
BbvI	GCAGC	6 cut(s)	300, 652, 750, 772, 1680, 1732
BccI	CCATC	3 cut(s)	1045, 1347, 1615
BceAI	ACGGC	1 cut(s)	1571
BciT130I	CCWGG	1 cut(s)	1630
BcnI	CCSGG	1 cut(s)	1620
BcoDI	GTCTC	2 cut(s)	667, 788
BfaI	CTAG	4 cut(s)	51, 825, 1266, 1389
BfmI	CTRYAG	3 cut(s)	764, 855, 1173
BfuAI	ACCTGC	1 cut(s)	280
BglII	AGATCT	2 cut(s)	748, 1409
BisI	GCNGC	6 cut(s)	314, 666, 761, 764, 1694, 1746
BlsI	GCNGC	6 cut(s)	315, 667, 762, 765, 1695, 1747
Bme1390I	CCNGG	2 cut(s)	1620, 1630
Bme18I	GGWCC	2 cut(s)	8, 1033
BmgT120I	GGNCC	5 cut(s)	8, 399, 1022, 1033, 1602
BmiI	GGNNCC	2 cut(s)	1035, 1604
BmrFI	CCNGG	2 cut(s)	1620, 1630
BmrI	ACTGGG	1 cut(s)	1517
BmsI	GCATC	6 cut(s)	865, 934, 1406, 1420, 1704, 1753
BmuI	ACTGGG	1 cut(s)	1517
BpiI	GAAGAC	2 cut(s)	312, 532
BplI	GAGNNNNNCTC	4 cut(s)	32, 64, 855, 887
Bpu14I	TTCGAA	1 cut(s)	481
BpuEI	CTTGAG	3 cut(s)	161, 1694, 1730
BpuMI	CCSGG	1 cut(s)	1620
Bsa29I	ATCGAT	1 cut(s)	942
BsaBI	GATNNNNATC	1 cut(s)	333
BsaHI	GRCGYC	1 cut(s)	255
BsaJI	CCNNGG	7 cut(s)	258, 588, 1025, 1326, 1587, 1619, 1629
BsaWI	WCCGGW	1 cut(s)	5
BsaXI	ACNNNNNCTCC	4 cut(s)	181, 211, 1105, 1135
Bsc4I	CCNNNNNNNGG	4 cut(s)	616, 1619, 1620, 1869
Bse1I	ACTGG	4 cut(s)	118, 530, 1300, 1512
Bse3DI	GCAATG	1 cut(s)	1287
Bse8I	GATNNNNATC	1 cut(s)	333
BseAI	TCCGGA	1 cut(s)	5
BseBI	CCWGG	1 cut(s)	1630
BseCI	ATCGAT	1 cut(s)	942
BseDI	CCNNGG	7 cut(s)	258, 588, 1025, 1326, 1587, 1619, 1629
BseGI	GGATG	2 cut(s)	250, 1358
BseJI	GATNNNNATC	1 cut(s)	333
BseLI	CCNNNNNNNGG	4 cut(s)	616, 1619, 1620, 1869
BseMI	GCAATG	1 cut(s)	1287
BseMII	CTCAG	2 cut(s)	509, 783
BseNI	ACTGG	4 cut(s)	118, 530, 1300, 1512
BseRI	GAGGAG	5 cut(s)	113, 116, 121, 202, 1691
BseXI	GCAGC	6 cut(s)	300, 652, 750, 772, 1680, 1732
BsgI	GTGCAG	2 cut(s)	635, 726
Bsh1285I	CGRYCG	1 cut(s)	19
BshFI	GGCC	6 cut(s)	31, 401, 853, 1024, 1603, 1862
BshVI	ATCGAT	1 cut(s)	942
BsiEI	CGRYCG	1 cut(s)	19
BsiHKAI	GWGCWC	1 cut(s)	849
BsiSI	CCGG	3 cut(s)	6, 1304, 1619
BslFI	GGGAC	1 cut(s)	266
BslI	CCNNNNNNNGG	4 cut(s)	616, 1619, 1620, 1869
BsmAI	GTCTC	2 cut(s)	667, 788
BsmBI	CGTCTC	1 cut(s)	788
BsmFI	GGGAC	1 cut(s)	266
BsmI	GAATGC	2 cut(s)	1466, 1747
BsnI	GGCC	6 cut(s)	31, 401, 853, 1024, 1603, 1862
Bsp119I	TTCGAA	1 cut(s)	481
Bsp1286I	GDGCHC	1 cut(s)	849
Bsp13I	TCCGGA	1 cut(s)	5
Bsp143I	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
Bsp19I	CCATGG	2 cut(s)	1025, 1326
BspANI	GGCC	6 cut(s)	31, 401, 853, 1024, 1603, 1862
BspCNI	CTCAG	2 cut(s)	508, 784
BspDI	ATCGAT	1 cut(s)	942
BspEI	TCCGGA	1 cut(s)	5
BspLI	GGNNCC	2 cut(s)	1035, 1604
BspMAI	CTGCAG	2 cut(s)	768, 1177
BspMI	ACCTGC	1 cut(s)	280
BspPI	GGATC	2 cut(s)	554, 1192
BspT104I	TTCGAA	1 cut(s)	481
BsrDI	GCAATG	1 cut(s)	1287
BsrI	ACTGG	4 cut(s)	118, 530, 1300, 1512
BssECI	CCNNGG	7 cut(s)	258, 588, 1025, 1326, 1587, 1619, 1629
BssMI	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	4 cut(s)	258, 588, 1025, 1326
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	1 cut(s)	1630
Bst4CI	ACNGT	3 cut(s)	859, 1096, 1106
Bst6I	CTCTTC	2 cut(s)	179, 188
BstACI	GRCGYC	1 cut(s)	255
BstAPI	GCANNNNNTGC	1 cut(s)	149
BstBI	TTCGAA	1 cut(s)	481
BstC8I	GCNNGC	5 cut(s)	655, 1285, 1301, 1440, 1698
BstDEI	CTNAG	4 cut(s)	495, 792, 1239, 1518
BstDSI	CCRYGG	3 cut(s)	1025, 1326, 1587
BstF5I	GGATG	2 cut(s)	250, 1358
BstKTI	GATC	7 cut(s)	19, 562, 751, 994, 1187, 1412, 1499
BstMAI	GTCTC	2 cut(s)	667, 788
BstMBI	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
BstMCI	CGRYCG	1 cut(s)	19
BstMWI	GCNNNNNNNGC	6 cut(s)	149, 278, 830, 1289, 1439, 1702
BstNI	CCWGG	1 cut(s)	1630
BstNSI	RCATGY	2 cut(s)	397, 1442
BstSCI	CCNGG	2 cut(s)	1618, 1628
BstSFI	CTRYAG	3 cut(s)	764, 855, 1173
BstV1I	GCAGC	6 cut(s)	300, 652, 750, 772, 1680, 1732
BstV2I	GAAGAC	2 cut(s)	312, 532
BstX2I	RGATCY	3 cut(s)	748, 1184, 1409
BstYI	RGATCY	3 cut(s)	748, 1184, 1409
Bsu15I	ATCGAT	1 cut(s)	942
BsuRI	GGCC	6 cut(s)	31, 401, 853, 1024, 1603, 1862
BsuTUI	ATCGAT	1 cut(s)	942
BtgI	CCRYGG	3 cut(s)	1025, 1326, 1587
BtsCI	GGATG	2 cut(s)	250, 1358
BtsIMutI	CAGTG	3 cut(s)	469, 1111, 1505
BveI	ACCTGC	1 cut(s)	280
Cac8I	GCNNGC	5 cut(s)	655, 1285, 1301, 1440, 1698
CaiI	CAGNNNCTG	1 cut(s)	1181
Cfr13I	GGNCC	5 cut(s)	8, 399, 1022, 1033, 1602
ClaI	ATCGAT	1 cut(s)	942
CseI	GACGC	4 cut(s)	141, 263, 287, 805
Csp6I	GTAC	5 cut(s)	432, 916, 981, 1309, 1616
CspCI	CAANNNNNGTGG	4 cut(s)	1395, 1430, 1569, 1604
CviQI	GTAC	5 cut(s)	432, 916, 981, 1309, 1616
DdeI	CTNAG	4 cut(s)	495, 792, 1239, 1518
DpnI	GATC	7 cut(s)	18, 561, 750, 993, 1186, 1411, 1498
DpnII	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
Eam1104I	CTCTTC	2 cut(s)	179, 188
EarI	CTCTTC	2 cut(s)	179, 188
Eco130I	CCWWGG	4 cut(s)	258, 588, 1025, 1326
Eco147I	AGGCCT	1 cut(s)	31
Eco47I	GGWCC	2 cut(s)	8, 1033
Eco57I	CTGAAG	3 cut(s)	213, 1067, 1209
EcoO109I	RGGNCCY	2 cut(s)	1033, 1602
EcoRI	GAATTC	1 cut(s)	1595
EcoRII	CCWGG	1 cut(s)	1628
EcoT14I	CCWWGG	4 cut(s)	258, 588, 1025, 1326
EcoT22I	ATGCAT	1 cut(s)	1440
ErhI	CCWWGG	4 cut(s)	258, 588, 1025, 1326
Esp3I	CGTCTC	1 cut(s)	788
FalI	AAGNNNNNCTT	2 cut(s)	1818, 1850
FaqI	GGGAC	1 cut(s)	266
FauNDI	CATATG	1 cut(s)	339
Fnu4HI	GCNGC	6 cut(s)	314, 666, 761, 764, 1694, 1746
FokI	GGATG	2 cut(s)	257, 1365
Fsp4HI	GCNGC	6 cut(s)	314, 666, 761, 764, 1694, 1746
FspBI	CTAG	4 cut(s)	51, 825, 1266, 1389
GluI	GCNGC	6 cut(s)	314, 666, 761, 764, 1694, 1746
HaeIII	GGCC	6 cut(s)	31, 401, 853, 1024, 1603, 1862
HapII	CCGG	3 cut(s)	6, 1304, 1619
HgaI	GACGC	4 cut(s)	141, 263, 287, 805
Hin1I	GRCGYC	1 cut(s)	255
HincII	GTYRAC	5 cut(s)	277, 514, 1245, 1333, 1581
HindII	GTYRAC	5 cut(s)	277, 514, 1245, 1333, 1581
HindIII	AAGCTT	3 cut(s)	569, 1661, 1865
HinfI	GANTC	7 cut(s)	409, 683, 1178, 1246, 1544, 1577, 1772
HpaII	CCGG	3 cut(s)	6, 1304, 1619
HphI	GGTGA	3 cut(s)	1200, 1602, 1679
Hpy99I	CGWCG	1 cut(s)	62
HpyAV	CCTTC	6 cut(s)	327, 835, 1024, 1103, 1649, 1675
HpyCH4III	ACNGT	3 cut(s)	859, 1096, 1106
HpyCH4IV	ACGT	1 cut(s)	169
HpyF10VI	GCNNNNNNNGC	6 cut(s)	149, 278, 830, 1289, 1439, 1702
HpyF3I	CTNAG	4 cut(s)	495, 792, 1239, 1518
HpySE526I	ACGT	1 cut(s)	169
Hsp92I	GRCGYC	1 cut(s)	255
Kpn2I	TCCGGA	1 cut(s)	5
Kzo9I	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
LmnI	GCTCC	2 cut(s)	262, 1732
Lsp1109I	GCAGC	6 cut(s)	300, 652, 750, 772, 1680, 1732
LweI	GCATC	6 cut(s)	865, 934, 1406, 1420, 1704, 1753
MaeI	CTAG	4 cut(s)	51, 825, 1266, 1389
MaeII	ACGT	1 cut(s)	169
MaeIII	GTNAC	5 cut(s)	580, 779, 908, 1090, 1474
MalI	GATC	7 cut(s)	18, 561, 750, 993, 1186, 1411, 1498
MboI	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
MflI	RGATCY	3 cut(s)	748, 1184, 1409
MhlI	GDGCHC	1 cut(s)	849
MlyI	GAGTC	3 cut(s)	1187, 1240, 1586
Mph1103I	ATGCAT	1 cut(s)	1440
MroI	TCCGGA	1 cut(s)	5
MroXI	GAANNNNTTC	3 cut(s)	1320, 1638, 1830
MseI	TTAA	5 cut(s)	642, 723, 1527, 1781, 1814
MspA1I	CMGCKG	1 cut(s)	763
MspI	CCGG	3 cut(s)	6, 1304, 1619
MspR9I	CCNGG	2 cut(s)	1620, 1630
Mva1269I	GAATGC	2 cut(s)	1466, 1747
MvaI	CCWGG	1 cut(s)	1630
MwoI	GCNNNNNNNGC	6 cut(s)	149, 278, 830, 1289, 1439, 1702
NciI	CCSGG	1 cut(s)	1620
NcoI	CCATGG	2 cut(s)	1025, 1326
NdeI	CATATG	1 cut(s)	339
NdeII	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
NlaIV	GGNNCC	2 cut(s)	1035, 1604
NmuCI	GTSAC	2 cut(s)	580, 779
NsiI	ATGCAT	1 cut(s)	1440
NspI	RCATGY	2 cut(s)	397, 1442
NspV	TTCGAA	1 cut(s)	481
PaeI	GCATGC	1 cut(s)	1442
PaqCI	CACCTGC	1 cut(s)	280
PceI	AGGCCT	1 cut(s)	31
PciI	ACATGT	1 cut(s)	393
PctI	GAATGC	2 cut(s)	1466, 1747
PdmI	GAANNNNTTC	3 cut(s)	1320, 1638, 1830
PfeI	GAWTC	4 cut(s)	409, 683, 1544, 1772
PkrI	GCNGC	6 cut(s)	315, 667, 762, 765, 1695, 1747
Ple19I	CGATCG	1 cut(s)	19
PleI	GAGTC	3 cut(s)	1186, 1240, 1585
PpsI	GAGTC	3 cut(s)	1186, 1240, 1585
PpuMI	RGGWCCY	1 cut(s)	1033
PscI	ACATGT	1 cut(s)	393
PsiI	TTATAA	1 cut(s)	815
Psp5II	RGGWCCY	1 cut(s)	1033
Psp6I	CCWGG	1 cut(s)	1628
PspGI	CCWGG	1 cut(s)	1628
PspN4I	GGNNCC	2 cut(s)	1035, 1604
PspPI	GGNCC	5 cut(s)	8, 399, 1022, 1033, 1602
PspPPI	RGGWCCY	1 cut(s)	1033
PstI	CTGCAG	2 cut(s)	768, 1177
PstNI	CAGNNNCTG	1 cut(s)	1181
PsuI	RGATCY	3 cut(s)	748, 1184, 1409
PvuI	CGATCG	1 cut(s)	19
PvuII	CAGCTG	1 cut(s)	763
RsaI	GTAC	5 cut(s)	433, 917, 982, 1310, 1617
RsaNI	GTAC	5 cut(s)	432, 916, 981, 1309, 1616
SaqAI	TTAA	5 cut(s)	642, 723, 1527, 1781, 1814
SatI	GCNGC	6 cut(s)	314, 666, 761, 764, 1694, 1746
Sau3AI	GATC	7 cut(s)	16, 559, 748, 991, 1184, 1409, 1496
Sau96I	GGNCC	5 cut(s)	8, 399, 1022, 1033, 1602
SchI	GAGTC	3 cut(s)	1187, 1240, 1586
ScrFI	CCNGG	2 cut(s)	1620, 1630
SduI	GDGCHC	1 cut(s)	849
SfaNI	GCATC	6 cut(s)	865, 934, 1406, 1420, 1704, 1753
SfcI	CTRYAG	3 cut(s)	764, 855, 1173
SfuI	TTCGAA	1 cut(s)	481
SinI	GGWCC	2 cut(s)	8, 1033
SmlI	CTYRAG	3 cut(s)	176, 1673, 1709
SmoI	CTYRAG	3 cut(s)	176, 1673, 1709
SphI	GCATGC	1 cut(s)	1442
SseBI	AGGCCT	1 cut(s)	31
SspMI	CTAG	4 cut(s)	51, 825, 1266, 1389
StuI	AGGCCT	1 cut(s)	31
StyD4I	CCNGG	2 cut(s)	1618, 1628
StyI	CCWWGG	4 cut(s)	258, 588, 1025, 1326
TaaI	ACNGT	3 cut(s)	859, 1096, 1106
TaiI	ACGT	1 cut(s)	172
TaqI	TCGA	6 cut(s)	15, 236, 372, 481, 873, 942
TfiI	GAWTC	4 cut(s)	409, 683, 1544, 1772
Tru1I	TTAA	5 cut(s)	642, 723, 1527, 1781, 1814
Tru9I	TTAA	5 cut(s)	642, 723, 1527, 1781, 1814
TscAI	CASTG	3 cut(s)	469, 1111, 1512
TseFI	GTSAC	2 cut(s)	580, 779
TseI	GCWGC	6 cut(s)	313, 665, 760, 763, 1693, 1745
Tsp45I	GTSAC	2 cut(s)	580, 779
TspDTI	ATGAA	4 cut(s)	489, 639, 887, 1329
TspRI	CASTG	3 cut(s)	469, 1111, 1512
VpaK11BI	GGWCC	2 cut(s)	8, 1033
XapI	RAATTY	4 cut(s)	127, 161, 894, 1595
XbaI	TCTAGA	1 cut(s)	1388
XceI	RCATGY	2 cut(s)	397, 1442
XmnI	GAANNNNTTC	3 cut(s)	1320, 1638, 1830
XspI	CTAG	4 cut(s)	51, 825, 1266, 1389
Zsp2I	ATGCAT	1 cut(s)	1440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.