Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0007051

Physical Location & Seq

Genomic Coordinates Forward (+)

1128583 .. 1132280

Gene Structure 3698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g01930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGTCGGGACCTCTTGATCGATTCGCCAGGCCTTGTTTTGAGGGGTTCTCGAGCCACGACGAAAGGAGGGAAAGGAAGTCGGATTTTGAGAACTCGGAGGAGGAGAGGAGGTCTGGAATTGGTAGGAATTTGAAGAAGAAAGCAATTAATGCGTCTACAAAATTCAGACGTTCCCTCAAGAAGAAGAGGAGGAAGAGTGGCGGTGGTAGTAGTTGTAGGCTGTCCACTTCTGTTTCGATTGAGGATGTAAGGGACGCCGAGGAGCTACGAGCTGTTGACGCATTTCGGCAGGCCCTTATCTCGGAAGACTTGCTGCCTCCAAGACATGATGACTATCATATGTTGTTGAGATTTTTGACAGCAAGGAAGTTCGATATCGAGAAAGCAAAGCACATGTGGGCCAATATGATTCAGTGGAGGAAAGATTTTGGTACTGATACAATTTTGGAGGATTTTGAGTTCGGTGAGTTGAGTGAAGTTCGAAAGCACTACCCTCAGTTTTATCATGGTGTTGACAAGGAGGGAAGACCTGTTTACTTTGAAAGGTTGGGGAAAGTTGATCCCAACAGGCTTATGCAAGTGACTTCTTTGGAACGGTATGTGAGATACCATGTGCAGGAGTATGAAAGGAGTTTTGCCATTAAGTTTCCTGCTTGCTCTATTGCTGCAAAGAGACATATAGATTCAAGTACAACAATTTTAGATGTTCAAGGCGTGGGTCTTAAAAACTTTACTAAGTCTGCACGAGATCTCACAGAGCGGCTGCAGAAGATTGATAGTGACAACTATCCTGAGACGCTTTGCCGAATGTTAATAATCAATGCTAGTTACGCTTTTAGGAAGGTGCTCTGGCCTACAGTTAAAGCATTCCTCGATGCCAGAACAACCAGCAAAATTCATGTTCTGGGTAACAAGTACCAGAGCAAATTGCTTGAAATAATCGATGCAAGTGAGTTGCCAGAATTTATGGGTGGTAGCTGTACCTGTTCCGATCAGGGAGGTTGTATGTCCTCTGATAAAGGGCCATGGAATGACCCAAACATACTGAAGATGGTACTTAATGGTGAAACACAATGTTTCAGACAAATAGTTACAATTTCAAACAGTGAAGGGGGGATAATTGCTTTTGATAAGCCACACAATCCTATGATAAGAAGTAGTGATACTTCTACCGCAGAGTCTGGATCTGAAGTGGAAGAAATGGGTTCACCTAAAGCAACTAGGAACTACTTACAACCTAAGTTGACTCCTGTTTGTGAAGAAGCCAGGGTAGTTGGCAAAGCAAGCGTTGCTACTGCCTTGTTGGAGTACGATGAACAGGTTCCGATGGTTGACAAGGCTGTGGATATGGGATGGAAGAAACCAGCATTACAACAAGCACTGCTTGCTTCTGGAGGGGCATCTTCTCCACAATCTGTGGGGAAAACAACTGATGGAATGCTTGCTAATATCTGGACTATGCTTGTGCCATTCTTTGTAACCCTTGTTTCGCTAATCCGATCTTTTTCACTGCGGGCAACTAAGAAACTCCAAAACTCTGTTTCTGATTCTGCCCACAACATTCCTGACCTAACCAATGAGTCAACTGCCGTGGAGGAATTCCGGCCCCCATCACCTGCACCAGGGTTATCCAGGGAAAACCTTCTCTCATCTGCTTTGAAGAAGCTTGGTGAGCTTGAGGAGAAGGTAGATATGCTGCGAGAAAAGCCTCTTGAGATGCCTTACGAGAAAGTGGAGCTACTGAATGCAGCTGTTTATCGTGTAGATGCGCTAGAATCAGAGTTAATTGCAACAAAAAAGGCTCTGCATGAGGCTTTAATGAGGCAGGAAGAACTTCTTGAATATATAGACAGGCAAGAACAGGCCAAGCTTCAGAAAAACCGGTCATGCTGCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008150 GO:0008525 GO:0008526 GO:0009653 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044424 GO:0044464 GO:0044703 GO:0048193 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0071702 GO:0071840 GO:0071944 GO:0120009 GO:0120010 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

633

Amino Acids

71.55

Weight (kDa)

8.14

Isoelectric Point (pI)

54.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	93 - 134	5.6e-06	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	162 - 325	1.1e-28	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19650 AT1G19650 AT1G19650 AT1G75370 AT1G75370
fragaria_vesca	FvH4_2g39420 FvH4_2g39420
malus_domestica	MD08G1026500.v1.1 MD15G1023300.v1.1
prunus_persica	Prupe.1G375200_v2.0.a1 Prupe.1G375200_v2.0.a1
pyrus_communis	pycom08g02050 pycom15g01930
rosa_chinensis	RchiOBHm_Chr6g0301561
rosa_laevigata	RLG00000011244
rosa_multiflora	Rmu_ssc0000452.1_g000004
rosa_roxburghii	Rroxscaffold_7G00166650
rosa_rugosa	Rorug06G0306100 Rorug06G0306200 Rorug06G0306300
rosa_samantha	Rh6AG419800 Rh6BG425800 Rh6CG432800 Rh6DG419000
rosa_wichuraiana	Rw6G036290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1624
Acc36I	ACCTGC	1 cut(s)	1624
AccBSI	CCGCTC	1 cut(s)	760
AccI	GTMKAC	1 cut(s)	155
AciI	CCGC	4 cut(s)	201, 760, 1173, 1513
AclWI	GGATC	2 cut(s)	554, 1192
AcsI	RAATTY	5 cut(s)	127, 161, 894, 962, 1598
AcuI	CTGAAG	3 cut(s)	1067, 1209, 1856
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	6 cut(s)	433, 691, 917, 982, 1056, 1310
AfiI	CCNNNNNNNGG	3 cut(s)	301, 616, 1622
AflIII	ACRYGT	1 cut(s)	393
AgeI	ACCGGT	1 cut(s)	1881
AgsI	TTSAA	8 cut(s)	133, 542, 687, 710, 935, 1101, 1660, 1841
AjnI	CCWGG	4 cut(s)	26, 1265, 1621, 1631
AloI	GAACNNNNNNTCC	2 cut(s)	443, 475
AluBI	AGCT	8 cut(s)	265, 272, 978, 1666, 1675, 1738, 1751, 1870
AluI	AGCT	8 cut(s)	265, 272, 978, 1666, 1675, 1738, 1751, 1870
Alw21I	GWGCWC	1 cut(s)	849
Alw26I	GTCTC	2 cut(s)	667, 788
AlwI	GGATC	2 cut(s)	554, 1192
AlwNI	CAGNNNCTG	1 cut(s)	1181
Ama87I	CYCGRG	1 cut(s)	49
AoxI	GGCC	7 cut(s)	29, 291, 399, 851, 1022, 1604, 1863
ApeKI	GCWGC	6 cut(s)	313, 665, 763, 1696, 1748, 1890
ApoI	RAATTY	5 cut(s)	127, 161, 894, 962, 1598
AseI	ATTAAT	1 cut(s)	147
AsiGI	ACCGGT	1 cut(s)	1881
Asp700I	GAANNNNTTC	3 cut(s)	1320, 1641, 1833
AspLEI	GCGC	1 cut(s)	1771
AspS9I	GGNCC	5 cut(s)	8, 292, 399, 1022, 1605
AsuHPI	GGTGA	5 cut(s)	476, 1076, 1200, 1605, 1682
AsuII	TTCGAA	1 cut(s)	481
AvaI	CYCGRG	1 cut(s)	49
AvaII	GGWCC	1 cut(s)	8
BarI	GAAGNNNNNNTAC	4 cut(s)	1147, 1150, 1179, 1182
BauI	CACGAG	1 cut(s)	744
BbsI	GAAGAC	2 cut(s)	312, 532
Bbv12I	GWGCWC	1 cut(s)	849
BbvI	GCAGC	6 cut(s)	300, 652, 750, 1683, 1760, 1877
BccI	CCATC	5 cut(s)	1045, 1321, 1347, 1427, 1618
BceAI	ACGGC	1 cut(s)	1574
BciT130I	CCWGG	4 cut(s)	28, 1267, 1623, 1633
BcoDI	GTCTC	2 cut(s)	667, 788
BfaI	CTAG	3 cut(s)	825, 1221, 1772
BfmI	CTRYAG	2 cut(s)	764, 855
BfuAI	ACCTGC	1 cut(s)	1624
BglII	AGATCT	1 cut(s)	748
BisI	GCNGC	7 cut(s)	314, 666, 761, 764, 1697, 1749, 1891
BlsI	GCNGC	7 cut(s)	315, 667, 762, 765, 1698, 1750, 1892
Bme1390I	CCNGG	4 cut(s)	28, 1267, 1623, 1633
Bme18I	GGWCC	1 cut(s)	8
BmeT110I	CYCGRG	1 cut(s)	49
BmgT120I	GGNCC	5 cut(s)	8, 292, 399, 1022, 1605
BmiI	GGNNCC	3 cut(s)	9, 1323, 1607
BmrFI	CCNGG	4 cut(s)	28, 1267, 1623, 1633
BmsI	GCATC	5 cut(s)	865, 934, 1409, 1707, 1756
BpiI	GAAGAC	2 cut(s)	312, 532
BplI	GAGNNNNNCTC	2 cut(s)	32, 64
BpmI	CTGGAG	1 cut(s)	1413
Bpu14I	TTCGAA	1 cut(s)	481
BpuEI	CTTGAG	3 cut(s)	161, 1697, 1733
Bsa29I	ATCGAT	2 cut(s)	19, 942
BsaBI	GATNNNNATC	1 cut(s)	333
BsaHI	GRCGYC	1 cut(s)	255
BsaJI	CCNNGG	6 cut(s)	258, 1025, 1266, 1590, 1622, 1632
BsaWI	WCCGGW	1 cut(s)	1881
BsaXI	ACNNNNNCTCC	4 cut(s)	95, 125, 181, 211
Bsc4I	CCNNNNNNNGG	3 cut(s)	301, 616, 1622
Bse118I	RCCGGY	1 cut(s)	1881
Bse8I	GATNNNNATC	1 cut(s)	333
BseBI	CCWGG	4 cut(s)	28, 1267, 1623, 1633
BseCI	ATCGAT	2 cut(s)	19, 942
BseDI	CCNNGG	6 cut(s)	258, 1025, 1266, 1590, 1622, 1632
BseGI	GGATG	2 cut(s)	250, 1358
BseJI	GATNNNNATC	1 cut(s)	333
BseLI	CCNNNNNNNGG	3 cut(s)	301, 616, 1622
BseMII	CTCAG	2 cut(s)	509, 783
BseRI	GAGGAG	6 cut(s)	113, 116, 121, 202, 275, 1694
BseXI	GCAGC	6 cut(s)	300, 652, 750, 1683, 1760, 1877
BsgI	GTGCAG	3 cut(s)	635, 726, 1602
BshFI	GGCC	7 cut(s)	31, 293, 401, 853, 1024, 1606, 1865
BshTI	ACCGGT	1 cut(s)	1881
BshVI	ATCGAT	2 cut(s)	19, 942
BsiHKAI	GWGCWC	1 cut(s)	849
BsiHKCI	CYCGRG	1 cut(s)	49
BsiSI	CCGG	2 cut(s)	1603, 1882
BslFI	GGGAC	2 cut(s)	21, 266
BslI	CCNNNNNNNGG	3 cut(s)	301, 616, 1622
BsmAI	GTCTC	2 cut(s)	667, 788
BsmBI	CGTCTC	1 cut(s)	788
BsmFI	GGGAC	2 cut(s)	21, 266
BsmI	GAATGC	3 cut(s)	866, 1443, 1750
BsnI	GGCC	7 cut(s)	31, 293, 401, 853, 1024, 1606, 1865
BsoBI	CYCGRG	1 cut(s)	49
Bsp119I	TTCGAA	1 cut(s)	481
Bsp1286I	GDGCHC	1 cut(s)	849
Bsp143I	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
Bsp19I	CCATGG	1 cut(s)	1025
BspACI	CCGC	4 cut(s)	201, 760, 1173, 1513
BspANI	GGCC	7 cut(s)	31, 293, 401, 853, 1024, 1606, 1865
BspCNI	CTCAG	2 cut(s)	508, 784
BspDI	ATCGAT	2 cut(s)	19, 942
BspLI	GGNNCC	3 cut(s)	9, 1323, 1607
BspMAI	CTGCAG	1 cut(s)	768
BspMI	ACCTGC	1 cut(s)	1624
BspPI	GGATC	2 cut(s)	554, 1192
BspT104I	TTCGAA	1 cut(s)	481
BsrBI	CCGCTC	1 cut(s)	760
BsrFI	RCCGGY	1 cut(s)	1881
BssAI	RCCGGY	1 cut(s)	1881
BssECI	CCNNGG	6 cut(s)	258, 1025, 1266, 1590, 1622, 1632
BssMI	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	1 cut(s)	1025
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	4 cut(s)	28, 1267, 1623, 1633
Bst4CI	ACNGT	3 cut(s)	597, 859, 1106
Bst6I	CTCTTC	2 cut(s)	179, 188
BstACI	GRCGYC	1 cut(s)	255
BstAPI	GCANNNNNTGC	3 cut(s)	149, 1289, 1385
BstBI	TTCGAA	1 cut(s)	481
BstC8I	GCNNGC	6 cut(s)	291, 655, 1285, 1386, 1443, 1515
BstDEI	CTNAG	5 cut(s)	495, 735, 792, 1239, 1521
BstDSI	CCRYGG	2 cut(s)	1025, 1590
BstENI	CCTNNNNNAGG	1 cut(s)	1620
BstF5I	GGATG	2 cut(s)	250, 1358
BstHHI	GCGC	1 cut(s)	1771
BstKTI	GATC	6 cut(s)	19, 562, 751, 994, 1187, 1502
BstMAI	GTCTC	2 cut(s)	667, 788
BstMBI	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
BstMWI	GCNNNNNNNGC	8 cut(s)	149, 278, 830, 1284, 1289, 1385, 1672, 1705
BstNI	CCWGG	4 cut(s)	28, 1267, 1623, 1633
BstNSI	RCATGY	1 cut(s)	397
BstSCI	CCNGG	4 cut(s)	26, 1265, 1621, 1631
BstSFI	CTRYAG	2 cut(s)	764, 855
BstV1I	GCAGC	6 cut(s)	300, 652, 750, 1683, 1760, 1877
BstV2I	GAAGAC	2 cut(s)	312, 532
BstX2I	RGATCY	2 cut(s)	748, 1184
BstYI	RGATCY	2 cut(s)	748, 1184
Bsu15I	ATCGAT	2 cut(s)	19, 942
BsuRI	GGCC	7 cut(s)	31, 293, 401, 853, 1024, 1606, 1865
BsuTUI	ATCGAT	2 cut(s)	19, 942
BtgI	CCRYGG	2 cut(s)	1025, 1590
BtsCI	GGATG	2 cut(s)	250, 1358
BtsI	GCAGTG	2 cut(s)	1379, 1508
BtsIMutI	CAGTG	4 cut(s)	419, 1111, 1379, 1508
BveI	ACCTGC	1 cut(s)	1624
Cac8I	GCNNGC	6 cut(s)	291, 655, 1285, 1386, 1443, 1515
CaiI	CAGNNNCTG	1 cut(s)	1181
CfoI	GCGC	1 cut(s)	1771
Cfr10I	RCCGGY	1 cut(s)	1881
Cfr13I	GGNCC	5 cut(s)	8, 292, 399, 1022, 1605
ClaI	ATCGAT	2 cut(s)	19, 942
CseI	GACGC	4 cut(s)	141, 263, 287, 805
Csp6I	GTAC	6 cut(s)	432, 690, 916, 981, 1055, 1309
CspAI	ACCGGT	1 cut(s)	1881
CspCI	CAANNNNNGTGG	2 cut(s)	1572, 1607
CviAII	CATG	8 cut(s)	326, 394, 506, 611, 899, 1026, 1808, 1887
CviQI	GTAC	6 cut(s)	432, 690, 916, 981, 1055, 1309
DdeI	CTNAG	5 cut(s)	495, 735, 792, 1239, 1521
DpnI	GATC	6 cut(s)	18, 561, 750, 993, 1186, 1501
DpnII	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
Eam1104I	CTCTTC	2 cut(s)	179, 188
EarI	CTCTTC	2 cut(s)	179, 188
Eco130I	CCWWGG	1 cut(s)	1025
Eco147I	AGGCCT	1 cut(s)	31
Eco32I	GATATC	1 cut(s)	376
Eco47I	GGWCC	1 cut(s)	8
Eco57I	CTGAAG	3 cut(s)	1067, 1209, 1856
Eco88I	CYCGRG	1 cut(s)	49
EcoNI	CCTNNNNNAGG	1 cut(s)	1620
EcoO109I	RGGNCCY	2 cut(s)	8, 292
EcoRI	GAATTC	1 cut(s)	1598
EcoRII	CCWGG	4 cut(s)	26, 1265, 1621, 1631
EcoRV	GATATC	1 cut(s)	376
EcoT14I	CCWWGG	1 cut(s)	1025
ErhI	CCWWGG	1 cut(s)	1025
Esp3I	CGTCTC	1 cut(s)	788
FaeI	CATG	8 cut(s)	329, 397, 509, 614, 902, 1029, 1811, 1890
FalI	AAGNNNNNCTT	4 cut(s)	1368, 1400, 1821, 1853
FaqI	GGGAC	2 cut(s)	21, 266
FatI	CATG	8 cut(s)	325, 393, 505, 610, 898, 1025, 1807, 1886
FauI	CCCGC	1 cut(s)	1506
FauNDI	CATATG	1 cut(s)	339
FblI	GTMKAC	1 cut(s)	155
Fnu4HI	GCNGC	7 cut(s)	314, 666, 761, 764, 1697, 1749, 1891
FokI	GGATG	2 cut(s)	257, 1365
Fsp4HI	GCNGC	7 cut(s)	314, 666, 761, 764, 1697, 1749, 1891
FspBI	CTAG	3 cut(s)	825, 1221, 1772
GlaI	GCGC	1 cut(s)	1770
GluI	GCNGC	7 cut(s)	314, 666, 761, 764, 1697, 1749, 1891
GsuI	CTGGAG	1 cut(s)	1413
HaeIII	GGCC	7 cut(s)	31, 293, 401, 853, 1024, 1606, 1865
HapII	CCGG	2 cut(s)	1603, 1882
HgaI	GACGC	4 cut(s)	141, 263, 287, 805
HhaI	GCGC	1 cut(s)	1771
Hin1I	GRCGYC	1 cut(s)	255
Hin1II	CATG	8 cut(s)	329, 397, 509, 614, 902, 1029, 1811, 1890
Hin6I	GCGC	1 cut(s)	1769
HinP1I	GCGC	1 cut(s)	1769
HincII	GTYRAC	5 cut(s)	277, 514, 1245, 1333, 1584
HindII	GTYRAC	5 cut(s)	277, 514, 1245, 1333, 1584
HindIII	AAGCTT	2 cut(s)	1664, 1868
HinfI	GANTC	8 cut(s)	21, 409, 683, 1178, 1246, 1547, 1580, 1775
HpaII	CCGG	2 cut(s)	1603, 1882
HphI	GGTGA	5 cut(s)	476, 1076, 1200, 1605, 1682
Hpy166II	GTNNAC	9 cut(s)	156, 225, 277, 514, 535, 1208, 1245, 1333, 1584
Hpy8I	GTNNAC	9 cut(s)	156, 225, 277, 514, 535, 1208, 1245, 1333, 1584
Hpy99I	CGWCG	1 cut(s)	62
HpyAV	CCTTC	4 cut(s)	835, 1103, 1652, 1678
HpyCH4III	ACNGT	3 cut(s)	597, 859, 1106
HpyCH4IV	ACGT	1 cut(s)	169
HpyF10VI	GCNNNNNNNGC	8 cut(s)	149, 278, 830, 1284, 1289, 1385, 1672, 1705
HpyF3I	CTNAG	5 cut(s)	495, 735, 792, 1239, 1521
HpySE526I	ACGT	1 cut(s)	169
Hsp92I	GRCGYC	1 cut(s)	255
Hsp92II	CATG	8 cut(s)	329, 397, 509, 614, 902, 1029, 1811, 1890
HspAI	GCGC	1 cut(s)	1769
Kzo9I	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
LmnI	GCTCC	2 cut(s)	262, 1735
Lsp1109I	GCAGC	6 cut(s)	300, 652, 750, 1683, 1760, 1877
LweI	GCATC	5 cut(s)	865, 934, 1409, 1707, 1756
MaeI	CTAG	3 cut(s)	825, 1221, 1772
MaeII	ACGT	1 cut(s)	169
MaeIII	GTNAC	6 cut(s)	580, 779, 827, 908, 1090, 1477
MalI	GATC	6 cut(s)	18, 561, 750, 993, 1186, 1501
MbiI	CCGCTC	1 cut(s)	760
MboI	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
MflI	RGATCY	2 cut(s)	748, 1184
MhlI	GDGCHC	1 cut(s)	849
MlyI	GAGTC	3 cut(s)	1187, 1240, 1589
MmeI	TCCRAC	2 cut(s)	60, 1284
MroXI	GAANNNNTTC	3 cut(s)	1320, 1641, 1833
MseI	TTAA	8 cut(s)	147, 642, 723, 812, 861, 1059, 1784, 1817
MspA1I	CMGCKG	1 cut(s)	1751
MspI	CCGG	2 cut(s)	1603, 1882
MspR9I	CCNGG	4 cut(s)	28, 1267, 1623, 1633
Mva1269I	GAATGC	3 cut(s)	866, 1443, 1750
MvaI	CCWGG	4 cut(s)	28, 1267, 1623, 1633
MwoI	GCNNNNNNNGC	8 cut(s)	149, 278, 830, 1284, 1289, 1385, 1672, 1705
NcoI	CCATGG	1 cut(s)	1025
NdeI	CATATG	1 cut(s)	339
NdeII	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
NlaIII	CATG	8 cut(s)	329, 397, 509, 614, 902, 1029, 1811, 1890
NlaIV	GGNNCC	3 cut(s)	9, 1323, 1607
NmeAIII	GCCGAG	1 cut(s)	283
NmuCI	GTSAC	2 cut(s)	580, 779
NspI	RCATGY	1 cut(s)	397
NspV	TTCGAA	1 cut(s)	481
PaeR7I	CTCGAG	1 cut(s)	49
PaqCI	CACCTGC	1 cut(s)	1624
PceI	AGGCCT	1 cut(s)	31
PciI	ACATGT	1 cut(s)	393
PctI	GAATGC	3 cut(s)	866, 1443, 1750
PdmI	GAANNNNTTC	3 cut(s)	1320, 1641, 1833
PfeI	GAWTC	5 cut(s)	21, 409, 683, 1547, 1775
PinAI	ACCGGT	1 cut(s)	1881
PkrI	GCNGC	7 cut(s)	315, 667, 762, 765, 1698, 1750, 1892
PleI	GAGTC	3 cut(s)	1186, 1240, 1588
PpsI	GAGTC	3 cut(s)	1186, 1240, 1588
PpuMI	RGGWCCY	1 cut(s)	8
PscI	ACATGT	1 cut(s)	393
PshBI	ATTAAT	1 cut(s)	147
Psp5II	RGGWCCY	1 cut(s)	8
Psp6I	CCWGG	4 cut(s)	26, 1265, 1621, 1631
PspGI	CCWGG	4 cut(s)	26, 1265, 1621, 1631
PspN4I	GGNNCC	3 cut(s)	9, 1323, 1607
PspPI	GGNCC	5 cut(s)	8, 292, 399, 1022, 1605
PspPPI	RGGWCCY	1 cut(s)	8
PstI	CTGCAG	1 cut(s)	768
PstNI	CAGNNNCTG	1 cut(s)	1181
PsuI	RGATCY	2 cut(s)	748, 1184
PvuII	CAGCTG	1 cut(s)	1751
RsaI	GTAC	6 cut(s)	433, 691, 917, 982, 1056, 1310
RsaNI	GTAC	6 cut(s)	432, 690, 916, 981, 1055, 1309
SaqAI	TTAA	8 cut(s)	147, 642, 723, 812, 861, 1059, 1784, 1817
SatI	GCNGC	7 cut(s)	314, 666, 761, 764, 1697, 1749, 1891
Sau3AI	GATC	6 cut(s)	16, 559, 748, 991, 1184, 1499
Sau96I	GGNCC	5 cut(s)	8, 292, 399, 1022, 1605
SchI	GAGTC	3 cut(s)	1187, 1240, 1589
ScrFI	CCNGG	4 cut(s)	28, 1267, 1623, 1633
SduI	GDGCHC	1 cut(s)	849
SfaNI	GCATC	5 cut(s)	865, 934, 1409, 1707, 1756
SfcI	CTRYAG	2 cut(s)	764, 855
Sfr274I	CTCGAG	1 cut(s)	49
SfuI	TTCGAA	1 cut(s)	481
SinI	GGWCC	1 cut(s)	8
SlaI	CTCGAG	1 cut(s)	49
SmlI	CTYRAG	4 cut(s)	49, 176, 1676, 1712
SmoI	CTYRAG	4 cut(s)	49, 176, 1676, 1712
SseBI	AGGCCT	1 cut(s)	31
SsiI	CCGC	4 cut(s)	201, 760, 1173, 1513
SspMI	CTAG	3 cut(s)	825, 1221, 1772
StuI	AGGCCT	1 cut(s)	31
StyD4I	CCNGG	4 cut(s)	26, 1265, 1621, 1631
StyI	CCWWGG	1 cut(s)	1025
TaaI	ACNGT	3 cut(s)	597, 859, 1106
TaiI	ACGT	1 cut(s)	172
TaqI	TCGA	8 cut(s)	19, 50, 236, 372, 378, 481, 873, 942
TatI	WGTACW	1 cut(s)	689
TauI	GCSGC	1 cut(s)	763
TfiI	GAWTC	5 cut(s)	21, 409, 683, 1547, 1775
Tru1I	TTAA	8 cut(s)	147, 642, 723, 812, 861, 1059, 1784, 1817
Tru9I	TTAA	8 cut(s)	147, 642, 723, 812, 861, 1059, 1784, 1817
TscAI	CASTG	4 cut(s)	419, 1111, 1386, 1515
TseFI	GTSAC	2 cut(s)	580, 779
TseI	GCWGC	6 cut(s)	313, 665, 763, 1696, 1748, 1890
Tsp45I	GTSAC	2 cut(s)	580, 779
TspDTI	ATGAA	3 cut(s)	639, 887, 1329
TspRI	CASTG	4 cut(s)	419, 1111, 1386, 1515
VpaK11BI	GGWCC	1 cut(s)	8
VspI	ATTAAT	1 cut(s)	147
XagI	CCTNNNNNAGG	1 cut(s)	1620
XapI	RAATTY	5 cut(s)	127, 161, 894, 962, 1598
XceI	RCATGY	1 cut(s)	397
XhoI	CTCGAG	1 cut(s)	49
XmiI	GTMKAC	1 cut(s)	155
XmnI	GAANNNNTTC	3 cut(s)	1320, 1641, 1833
XspI	CTAG	3 cut(s)	825, 1221, 1772

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.