MD08G1026600.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016775
Physical Location & Seq
Reverse (-)
1940866 .. 1942239
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1026600.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGTGGTGCTTGTCGCCGAAAACCATACTATCGGCGGCCGCCTCACTCGCCGCCTCCGCAATCCTGTTCAAAACGATTGCAAACGACCTCATCCCGGAAGCCTTCCAAAAGTACTTCTACTCCGGCCTCCAAAAGCTCTCCTCCCAACTCACCGTCGTTGTGGAGGAGCTCGACGGCCTGACCCCGAACCAGATGTTCGAGGCGGCCAACCTCTACCTCGGCGCCAAGGTCTCCGCTGCTACGCGGAGGATCAAAGTCAACAAGCCCGAAAAAGAGGAGCAGTTTCTGATCACCATTGATAAGAATCAAGGCATCGTTGATTCTTTCAACGGTGTAGATTTCAAGTGGGTTTTGATAAGCACCGAAAAACCGAACAGTAAACGTGGCGGCGAAAATTCGCAGTCGGAGGTCCGACAGTTAGAGCTCAGCTTCCATAAGAAGCACAGGGACATGGTGCTGAGATCTTACTTGCCCTACATTCTACAGAAATCCAAAGAAATTAAAGAGGAGAGAAAGGTGGTGAAGCTTCACACGGTAGATTACAATGGCACGGATTACTGGAGCTCCATCAACCTTGATCATCCGGCGAGTTTCGAAACAATGGCGATGGAGCCGGAGATGAAGGCGGCGTTGGTGGAGGACCTCGAGAGGTTTAGAAAGAGGAAGGAGTATTATAGGAGGGTAGGAAAGGCTTGGAAAAGAGGGTACTTGTTGTACGGACCGCCCGGCACGGGAAAGTCGAGCTTGGTTGCGGCCATGGCTAATTATCTCAAGTTTGATATTTTTGACTTGGATTTGAGGGAGGTTCAGTGCAATTCTGATCTGCGAAGGTTACTGATCGGAACGAAGAGCCGGTCGATATTGGTGATCGAGGACATTGATTGTTCAGTTGAGTTGCAGAACCGAGATTCGGAGAACGAGCCCAACGCCGTGGAAGATGACAAGATCAGCCTCTCTGCTGTATTGAACTTCATCGACGGACTCTGGTCGACCTGTGGAGAGGAGAGAATTGTCGTGTTTACGACGAATCACAAGGACCGGCTTGATCCAGCGTTGCTGCGACCTGGCCGGATGGACCTGCACATACACATGTCCTACTGCACCTTCGGTGGTTTCAAGACATTGGCATATAATTACCTGCTGATTCACGAGCACACACTTTTCGGAGAAATTGAGATGTTGCTCGACAGGGCACAAGCAACGCCTGCTGAAGTTGCCGGAGAGCTGATGACGAGCGATGATGCTCATGTTTCCCTTCAAGGTCTTATCGAATTCCTACGAGGAAAGACTGATAGAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

434

Amino Acids

49.25

Weight (kDa)

6.12

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 31 - 119 1.2e-21 Domain associated at C-terminal with AAA
AAA PF00004 236 - 365 1.2e-19 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 367 - 428 7e-20 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39430
malus_domestica MD08G1026600.v1.1 MD08G1026700.v1.1
prunus_persica Prupe.1G375300_v2.0.a1 Prupe.1G375300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0301551
rosa_laevigata RLG00000011245
rosa_multiflora Rmu_ssc0000452.1_g000001
rosa_roxburghii Rroxscaffold_7G00166660
rosa_rugosa Rorug06G0306000
rosa_samantha Rh6AG419700 Rh6BG425700 Rh6DG419100
rosa_wichuraiana Rw6G036280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1086, 1146
AccB1I GGYRCC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 989
AccII CGCG 1 cut(s) 244
AclWI GGATC 2 cut(s) 257, 1040
AcoI YGGCCR 4 cut(s) 36, 204, 753, 1066
AcsI RAATTY 2 cut(s) 394, 1271
AcuI CTGAAG 1 cut(s) 1230
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 113, 707, 716
AfiI CCNNNNNNNGG 3 cut(s) 94, 731, 910
AflIII ACRYGT 1 cut(s) 1089
AgsI TTSAA 6 cut(s) 70, 328, 343, 967, 1117, 1259
AjnI CCWGG 1 cut(s) 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 8 cut(s) 136, 169, 424, 429, 526, 564, 744, 1225
AluI AGCT 8 cut(s) 136, 169, 424, 429, 526, 564, 744, 1225
Alw21I GWGCWC 4 cut(s) 171, 426, 566, 1155
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 2 cut(s) 257, 1040
AlwNI CAGNNNCTG 1 cut(s) 286
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 6 cut(s) 36, 124, 175, 204, 753, 1066
ApeKI GCWGC 2 cut(s) 236, 1057
ApoI RAATTY 2 cut(s) 394, 1271
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 5 cut(s) 409, 640, 719, 1036, 1075
AsuC2I CCSGG 2 cut(s) 95, 726
AsuHPI GGTGA 4 cut(s) 142, 283, 532, 877
AsuII TTCGAA 1 cut(s) 594
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 5 cut(s) 409, 640, 719, 1036, 1075
BaeGI GKGCMC 1 cut(s) 1195
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 4 cut(s) 171, 426, 566, 924
BauI CACGAG 1 cut(s) 1148
Bbv12I GWGCWC 4 cut(s) 171, 426, 566, 1155
BbvI GCAGC 2 cut(s) 223, 1044
BccI CCATC 3 cut(s) 575, 601, 1066
BceAI ACGGC 2 cut(s) 190, 914
BciT130I CCWGG 1 cut(s) 1065
BclI TGATCA 2 cut(s) 288, 577
BcnI CCSGG 2 cut(s) 95, 726
BcoDI GTCTC 1 cut(s) 235
BfmI CTRYAG 1 cut(s) 482
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 2 cut(s) 1086, 1146
BglII AGATCT 1 cut(s) 461
BisI GCNGC 9 cut(s) 36, 39, 51, 204, 237, 388, 627, 753, 1058
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 9 cut(s) 37, 40, 52, 205, 238, 389, 628, 754, 1059
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 95, 726, 1065
Bme18I GGWCC 5 cut(s) 409, 640, 719, 1036, 1075
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 5 cut(s) 409, 640, 719, 1036, 1075
BmiI GGNNCC 2 cut(s) 223, 612
BmrFI CCNGG 3 cut(s) 95, 726, 1065
BmsI GCATC 2 cut(s) 321, 1231
BoxI GACNNNNGTC 1 cut(s) 985
BpmI CTGGAG 1 cut(s) 580
Bpu1102I GCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 2 cut(s) 95, 726
BsaBI GATNNNNATC 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 4 cut(s) 217, 225, 756, 930
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 548, 578, 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 731, 910
Bse118I RCCGGY 2 cut(s) 852, 1038
Bse1I ACTGG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 303
BseBI CCWGG 1 cut(s) 1065
BseDI CCNNGG 4 cut(s) 217, 225, 756, 930
BseGI GGATG 3 cut(s) 90, 580, 1077
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 3 cut(s) 94, 731, 910
BseMII CTCAG 2 cut(s) 439, 449
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 5 cut(s) 130, 179, 290, 521, 1016
BseSI GKGCMC 1 cut(s) 1195
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 223, 1044
BsgI GTGCAG 2 cut(s) 1064, 1084
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 2 cut(s) 39, 857
BshFI GGCC 6 cut(s) 38, 126, 177, 206, 755, 1068
BshNI GGYRCC 1 cut(s) 221
BsiEI CGRYCG 2 cut(s) 39, 857
BsiHKAI GWGCWC 4 cut(s) 171, 426, 566, 1155
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 9 cut(s) 95, 123, 584, 614, 726, 853, 1039, 1069, 1218
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 3 cut(s) 94, 731, 910
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 461
BsnI GGCC 6 cut(s) 38, 126, 177, 206, 755, 1068
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 594
Bsp1286I GDGCHC 6 cut(s) 171, 426, 566, 924, 1155, 1195
Bsp143I GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
Bsp1720I GCTNAGC 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 756
BspANI GGCC 6 cut(s) 38, 126, 177, 206, 755, 1068
BspCNI CTCAG 2 cut(s) 438, 450
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 223, 612
BspMI ACCTGC 2 cut(s) 1086, 1146
BspPI GGATC 2 cut(s) 257, 1040
BspQI GCTCTTC 1 cut(s) 842
BspT104I TTCGAA 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 235
BsrFI RCCGGY 2 cut(s) 852, 1038
BsrI ACTGG 1 cut(s) 563
BssAI RCCGGY 2 cut(s) 852, 1038
BssECI CCNNGG 4 cut(s) 217, 225, 756, 930
BssMI GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 1148
BssT1I CCWWGG 2 cut(s) 225, 756
Bst2BI CACGAG 1 cut(s) 1148
Bst2UI CCWGG 1 cut(s) 1065
Bst4CI ACNGT 5 cut(s) 154, 332, 377, 417, 535
Bst6I CTCTTC 1 cut(s) 842
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 1206
BstDEI CTNAG 2 cut(s) 425, 458
BstDSI CCRYGG 2 cut(s) 756, 930
BstF5I GGATG 3 cut(s) 90, 580, 1077
BstFNI CGCG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 9 cut(s) 252, 291, 464, 580, 823, 840, 870, 948, 1048
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
BstMCI CGRYCG 2 cut(s) 39, 857
BstMWI GCNNNNNNNGC 5 cut(s) 47, 56, 758, 1205, 1214
BstNI CCWGG 1 cut(s) 1065
BstNSI RCATGY 1 cut(s) 1093
BstPAI GACNNNNGTC 1 cut(s) 985
BstSCI CCNGG 3 cut(s) 93, 724, 1063
BstSFI CTRYAG 1 cut(s) 482
BstSLI GKGCMC 1 cut(s) 1195
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 223, 1044
BstX2I RGATCY 1 cut(s) 461
BstXI CCANNNNNNTGG 1 cut(s) 931
BstYI RGATCY 1 cut(s) 461
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 6 cut(s) 38, 126, 177, 206, 755, 1068
BtgI CCRYGG 2 cut(s) 756, 930
BtgZI GCGATG 2 cut(s) 620, 1251
BtsCI GGATG 3 cut(s) 90, 580, 1077
BtsIMutI CAGTG 1 cut(s) 815
BveI ACCTGC 2 cut(s) 1086, 1146
Cac8I GCNNGC 1 cut(s) 1206
CaiI CAGNNNCTG 1 cut(s) 286
CciNI GCGGCCGC 1 cut(s) 36
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 2 cut(s) 852, 1038
Cfr13I GGNCC 5 cut(s) 409, 640, 719, 1036, 1075
CpoI CGGWCCG 1 cut(s) 719
Csp6I GTAC 3 cut(s) 112, 706, 715
CspI CGGWCCG 1 cut(s) 719
CviAII CATG 4 cut(s) 451, 757, 1090, 1247
CviQI GTAC 3 cut(s) 112, 706, 715
DdeI CTNAG 2 cut(s) 425, 458
DinI GGCGCC 1 cut(s) 223
DpnI GATC 9 cut(s) 251, 290, 463, 579, 822, 839, 869, 947, 1047
DpnII GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
EaeI YGGCCR 4 cut(s) 36, 204, 753, 1066
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 842
EarI CTCTTC 1 cut(s) 842
Ecl136II GAGCTC 3 cut(s) 169, 424, 564
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 225, 756
Eco24I GRGCYC 4 cut(s) 171, 426, 566, 924
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 5 cut(s) 409, 640, 719, 1036, 1075
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 3 cut(s) 169, 424, 564
Eco57I CTGAAG 1 cut(s) 1230
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 3 cut(s) 169, 424, 564
EcoO109I RGGNCCY 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 1271
EcoRII CCWGG 1 cut(s) 1063
EcoT14I CCWWGG 2 cut(s) 225, 756
EcoT38I GRGCYC 4 cut(s) 171, 426, 566, 924
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 225, 756
FaeI CATG 4 cut(s) 454, 760, 1093, 1250
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 1 cut(s) 461
FatI CATG 4 cut(s) 450, 756, 1089, 1246
FbaI TGATCA 2 cut(s) 288, 577
FblI GTMKAC 1 cut(s) 989
Fnu4HI GCNGC 9 cut(s) 36, 39, 51, 204, 237, 388, 627, 753, 1058
FokI GGATG 3 cut(s) 77, 567, 1084
FriOI GRGCYC 4 cut(s) 171, 426, 566, 924
Fsp4HI GCNGC 9 cut(s) 36, 39, 51, 204, 237, 388, 627, 753, 1058
GlaI GCGC 1 cut(s) 223
GluI GCNGC 9 cut(s) 36, 39, 51, 204, 237, 388, 627, 753, 1058
GsuI CTGGAG 1 cut(s) 580
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 6 cut(s) 38, 126, 177, 206, 755, 1068
HapII CCGG 9 cut(s) 95, 123, 584, 614, 726, 853, 1039, 1069, 1218
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 454, 760, 1093, 1250
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 2 cut(s) 259, 990
HindII GTYRAC 2 cut(s) 259, 990
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 6 cut(s) 304, 320, 908, 981, 1027, 1144
HpaII CCGG 9 cut(s) 95, 123, 584, 614, 726, 853, 1039, 1069, 1218
HphI GGTGA 4 cut(s) 142, 283, 532, 877
Hpy166II GTNNAC 4 cut(s) 259, 380, 990, 1020
Hpy188I TCNGA 7 cut(s) 288, 406, 413, 820, 842, 913, 1166
Hpy188III TCNNGA 3 cut(s) 646, 1117, 1148
Hpy8I GTNNAC 4 cut(s) 259, 380, 990, 1020
Hpy99I CGWCG 4 cut(s) 158, 176, 980, 1027
HpyAV CCTTC 6 cut(s) 112, 616, 658, 822, 1114, 1265
HpyCH4III ACNGT 5 cut(s) 154, 332, 377, 417, 535
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 5 cut(s) 80, 813, 898, 1081, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 56, 758, 1205, 1214
HpyF3I CTNAG 2 cut(s) 425, 458
HpySE526I ACGT 1 cut(s) 382
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 454, 760, 1093, 1250
HspAI GCGC 1 cut(s) 222
KasI GGCGCC 1 cut(s) 221
Ksp22I TGATCA 2 cut(s) 288, 577
Kzo9I GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
LguI GCTCTTC 1 cut(s) 842
LmnI GCTCC 5 cut(s) 166, 277, 561, 569, 610
Lsp1109I GCAGC 2 cut(s) 223, 1044
LweI GCATC 2 cut(s) 321, 1231
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 831
MalI GATC 9 cut(s) 251, 290, 463, 579, 822, 839, 869, 947, 1047
MboI GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
MboII GAAGA 2 cut(s) 859, 947
MflI RGATCY 1 cut(s) 461
MhlI GDGCHC 6 cut(s) 171, 426, 566, 924, 1155, 1195
MluCI AATT 8 cut(s) 394, 498, 763, 814, 1008, 1132, 1170, 1271
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 975
MmeI TCCRAC 2 cut(s) 384, 436
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 1088
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 9 cut(s) 95, 123, 584, 614, 726, 853, 1039, 1069, 1218
MspR9I CCNGG 3 cut(s) 95, 726, 1065
MvaI CCWGG 1 cut(s) 1065
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 5 cut(s) 47, 56, 758, 1205, 1214
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 95, 726
NcoI CCATGG 1 cut(s) 756
NdeII GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
NlaIII CATG 4 cut(s) 454, 760, 1093, 1250
NlaIV GGNNCC 2 cut(s) 223, 612
NmeAIII GCCGAG 1 cut(s) 198
NotI GCGGCCGC 1 cut(s) 36
NspI RCATGY 1 cut(s) 1093
NspV TTCGAA 1 cut(s) 594
PaeR7I CTCGAG 1 cut(s) 644
PciI ACATGT 1 cut(s) 1089
PciSI GCTCTTC 1 cut(s) 842
PcsI WCGNNNNNNNCGW 1 cut(s) 737
PfeI GAWTC 5 cut(s) 304, 320, 908, 1027, 1144
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 9 cut(s) 37, 40, 52, 205, 238, 389, 628, 754, 1059
PleI GAGTC 1 cut(s) 975
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 975
PpuMI RGGWCCY 1 cut(s) 640
PscI ACATGT 1 cut(s) 1089
PshAI GACNNNNGTC 1 cut(s) 985
Psp124BI GAGCTC 3 cut(s) 171, 426, 566
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 1 cut(s) 1063
PspGI CCWGG 1 cut(s) 1063
PspN4I GGNNCC 2 cut(s) 223, 612
PspPI GGNCC 5 cut(s) 409, 640, 719, 1036, 1075
PspPPI RGGWCCY 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 286
PsuI RGATCY 1 cut(s) 461
RsaI GTAC 3 cut(s) 113, 707, 716
RsaNI GTAC 3 cut(s) 112, 706, 715
RseI CAYNNNNRTG 1 cut(s) 1088
Rsr2I CGGWCCG 1 cut(s) 719
RsrII CGGWCCG 1 cut(s) 719
SacI GAGCTC 3 cut(s) 171, 426, 566
SalI GTCGAC 1 cut(s) 988
SapI GCTCTTC 1 cut(s) 842
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 9 cut(s) 36, 39, 51, 204, 237, 388, 627, 753, 1058
Sau3AI GATC 9 cut(s) 249, 288, 461, 577, 820, 837, 867, 945, 1045
Sau96I GGNCC 5 cut(s) 409, 640, 719, 1036, 1075
ScaI AGTACT 1 cut(s) 113
SchI GAGTC 1 cut(s) 975
ScrFI CCNGG 3 cut(s) 95, 726, 1065
SduI GDGCHC 6 cut(s) 171, 426, 566, 924, 1155, 1195
SfaNI GCATC 2 cut(s) 321, 1231
SfcI CTRYAG 1 cut(s) 482
SfoI GGCGCC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 644
SfuI TTCGAA 1 cut(s) 594
SinI GGWCC 5 cut(s) 409, 640, 719, 1036, 1075
SlaI CTCGAG 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 1088
SmlI CTYRAG 2 cut(s) 644, 770
SmoI CTYRAG 2 cut(s) 644, 770
Sse9I AATT 8 cut(s) 394, 498, 763, 814, 1008, 1132, 1170, 1271
SspDI GGCGCC 1 cut(s) 221
SstI GAGCTC 3 cut(s) 171, 426, 566
StyD4I CCNGG 3 cut(s) 93, 724, 1063
StyI CCWWGG 2 cut(s) 225, 756
TaaI ACNGT 5 cut(s) 154, 332, 377, 417, 535
TaiI ACGT 1 cut(s) 385
TasI AATT 8 cut(s) 394, 498, 763, 814, 1008, 1132, 1170, 1271
TatI WGTACW 1 cut(s) 111
TauI GCSGC 7 cut(s) 38, 41, 53, 206, 390, 629, 755
TfiI GAWTC 5 cut(s) 304, 320, 908, 1027, 1144
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 815
TseI GCWGC 2 cut(s) 236, 1057
TspDTI ATGAA 2 cut(s) 635, 961
TspGWI ACGGA 3 cut(s) 566, 732, 993
TspRI CASTG 1 cut(s) 815
VpaK11BI GGWCC 5 cut(s) 409, 640, 719, 1036, 1075
XapI RAATTY 2 cut(s) 394, 1271
XceI RCATGY 1 cut(s) 1093
XhoI CTCGAG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 989
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.