MD08G1026700.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016775
Physical Location & Seq
Reverse (-)
1948721 .. 1951372
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1026700.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGTGGTGCTTGTCGCAGGAAAGCCTACCGTCGCCAAAAACCATGCTATCGGCGGCCGCCTCACTCGCCGCCTCCGCAATCCTGTTCAAAACGATTGCAAACGACCTCATCCCGGAAGCCCTCCAAACGTCCTTCTACTCGCACCTCCAAAAGCTCTCCTCCCAACTCACCGTTGTTGTGGAGGAGATCGACGGCCTGACCCCGAACAAGATGTTCGAGACGGCCAACCTTTACCTCGGCGCCAAGGTCTCCGCTGCTATGCGGAGGATCAAAGTCAACAAGCCCGAAAAAGATGAGCAGTTTCTGATCACCATTGATAAGAATCAAGGCATCGTTGATTCTTTCAACGGTGTAGATTTCAAGTGGGTTTTGATAAGCACCGAAAAACCAAACAGTAACCGTGGTGGCGAAAATTCACAGTCGGAGGTTCGATTGTTAGAGCTCAGCTTCCATAAGAAGCAGGTAGCAGAGCTATTACAAAGGCTTTTTGGTAAAGGAAACACCCCCAGTGGAAATTCTAGCCACGAACAGCTTCACACGGTAGATTACAATGGCACGGATTACTGGCGCTCCATCAACCTTGATCATCCGGCGAGTTTCGAAACAACGGCGATGGAGCCGGAGATGAAGGCGGCATTGGTGGAGGACCTCGAGAGGTTTAGAAAGAGGAATGAGTATTATATGAGGGTAGGAAAGGATTGGAAAAGAGGGTACTTTTTGTACGGATTGCCCGGCATGGGAAAGTCGAGCTTGGTTGCGGCCATGGCTAATTATCTCAAGTTTGATATTTTTGACTTGGATTTGAGGGAGGTTCAGTGCAATTCTGATCTGAGAAGGTTACTGATCGGAACAAAGAGCCGGTCGATATTGGTGATCGAGGACATTGATTGTTCAGTTGAGTTGCAAAACAGAGATTCGGAGAACAAGCCCAAAGCCGCGGAAGATAACAAGATCAGCATCTCTGCTATATTGAACTTCATCGACGGACTCTGGTCGACCTGTGAAGAGGAGAGAATTGTCGTGTTTACGACAAATCACAAGGACCGGCTTGATCCAGCGTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

356

Amino Acids

40.14

Weight (kDa)

5.67

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 41 - 125 6.3e-21 Domain associated at C-terminal with AAA
AAA PF00004 239 - 355 1.1e-13 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39430
malus_domestica MD08G1026600.v1.1 MD08G1026700.v1.1
prunus_persica Prupe.1G375300_v2.0.a1 Prupe.1G375300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0301551
rosa_laevigata RLG00000011245
rosa_multiflora Rmu_ssc0000452.1_g000001
rosa_roxburghii Rroxscaffold_7G00166660
rosa_rugosa Rorug06G0306000
rosa_samantha Rh6AG419700 Rh6BG425700 Rh6DG419100
rosa_wichuraiana Rw6G036280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 995
AccII CGCG 1 cut(s) 938
AclWI GGATC 2 cut(s) 275, 1046
AcoI YGGCCR 3 cut(s) 54, 222, 759
AcsI RAATTY 2 cut(s) 412, 514
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 713, 722
AfiI CCNNNNNNNGG 2 cut(s) 112, 737
AgsI TTSAA 4 cut(s) 88, 346, 361, 973
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AluBI AGCT 6 cut(s) 154, 442, 447, 472, 532, 750
AluI AGCT 6 cut(s) 154, 442, 447, 472, 532, 750
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 2 cut(s) 212, 253
AlwI GGATC 2 cut(s) 275, 1046
AlwNI CAGNNNCTG 1 cut(s) 304
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 4 cut(s) 54, 193, 222, 759
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 2 cut(s) 412, 514
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 2 cut(s) 242, 570
AspS9I GGNCC 2 cut(s) 646, 1042
AsuC2I CCSGG 2 cut(s) 113, 732
AsuHPI GGTGA 3 cut(s) 160, 301, 883
AsuII TTCGAA 1 cut(s) 600
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 2 cut(s) 646, 1042
BanI GGYRCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 581, 607
BceAI ACGGC 3 cut(s) 208, 237, 624
BclI TGATCA 2 cut(s) 306, 583
BcnI CCSGG 2 cut(s) 113, 732
BcoDI GTCTC 2 cut(s) 212, 253
BfaI CTAG 1 cut(s) 519
BfoI RGCGCY 2 cut(s) 243, 571
BfuAI ACCTGC 1 cut(s) 451
BisI GCNGC 7 cut(s) 54, 57, 69, 255, 633, 759, 936
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 7 cut(s) 55, 58, 70, 256, 634, 760, 937
Bme1390I CCNGG 2 cut(s) 113, 732
Bme18I GGWCC 2 cut(s) 646, 1042
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 646, 1042
BmiI GGNNCC 2 cut(s) 241, 618
BmrFI CCNGG 2 cut(s) 113, 732
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 2 cut(s) 339, 966
BmuI ACTGGG 1 cut(s) 501
BoxI GACNNNNGTC 1 cut(s) 991
Bpu1102I GCTNAGC 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 2 cut(s) 113, 732
BsaBI GATNNNNATC 2 cut(s) 321, 956
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 5 cut(s) 235, 243, 400, 762, 936
BsaXI ACNNNNNCTCC 4 cut(s) 554, 584, 800, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 737
Bse118I RCCGGY 2 cut(s) 858, 1044
Bse1I ACTGG 2 cut(s) 507, 569
Bse8I GATNNNNATC 2 cut(s) 321, 956
BseDI CCNNGG 5 cut(s) 235, 243, 400, 762, 936
BseGI GGATG 2 cut(s) 108, 586
BseJI GATNNNNATC 2 cut(s) 321, 956
BseLI CCNNNNNNNGG 2 cut(s) 112, 737
BseMII CTCAG 2 cut(s) 457, 821
BseNI ACTGG 2 cut(s) 507, 569
BseRI GAGGAG 3 cut(s) 148, 197, 1022
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 938
Bsh1285I CGRYCG 2 cut(s) 57, 863
BshFI GGCC 4 cut(s) 56, 195, 224, 761
BshNI GGYRCC 1 cut(s) 239
BsiEI CGRYCG 2 cut(s) 57, 863
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 6 cut(s) 113, 590, 620, 732, 859, 1045
BslI CCNNNNNNNGG 2 cut(s) 112, 737
BsmAI GTCTC 2 cut(s) 212, 253
BsmBI CGTCTC 1 cut(s) 212
BsnI GGCC 4 cut(s) 56, 195, 224, 761
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 650
Bsp119I TTCGAA 1 cut(s) 600
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
Bsp1720I GCTNAGC 1 cut(s) 443
Bsp19I CCATGG 1 cut(s) 762
BspANI GGCC 4 cut(s) 56, 195, 224, 761
BspCNI CTCAG 2 cut(s) 456, 822
BspFNI CGCG 1 cut(s) 938
BspLI GGNNCC 2 cut(s) 241, 618
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 2 cut(s) 275, 1046
BspT104I TTCGAA 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 253
BsrFI RCCGGY 2 cut(s) 858, 1044
BsrI ACTGG 2 cut(s) 507, 569
BssAI RCCGGY 2 cut(s) 858, 1044
BssECI CCNNGG 5 cut(s) 235, 243, 400, 762, 936
BssMI GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 243, 762
Bst4CI ACNGT 7 cut(s) 30, 172, 350, 395, 401, 420, 541
Bst6I CTCTTC 1 cut(s) 999
BstACI GRCGYC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 600
BstDEI CTNAG 2 cut(s) 443, 830
BstDSI CCRYGG 3 cut(s) 400, 762, 936
BstF5I GGATG 2 cut(s) 108, 586
BstFNI CGCG 1 cut(s) 938
BstH2I RGCGCY 2 cut(s) 243, 571
BstHHI GCGC 2 cut(s) 242, 570
BstKTI GATC 9 cut(s) 189, 270, 309, 586, 829, 846, 876, 954, 1054
BstMAI GTCTC 2 cut(s) 212, 253
BstMBI GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
BstMCI CGRYCG 2 cut(s) 57, 863
BstMWI GCNNNNNNNGC 3 cut(s) 65, 74, 764
BstPAI GACNNNNGTC 1 cut(s) 991
BstSCI CCNGG 2 cut(s) 111, 730
BstUI CGCG 1 cut(s) 938
BstV1I GCAGC 1 cut(s) 241
BstZI CGGCCG 1 cut(s) 54
BsuRI GGCC 4 cut(s) 56, 195, 224, 761
BtgI CCRYGG 3 cut(s) 400, 762, 936
BtgZI GCGATG 1 cut(s) 626
BtsCI GGATG 2 cut(s) 108, 586
BtsIMutI CAGTG 2 cut(s) 514, 821
BveI ACCTGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 304
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 2 cut(s) 242, 570
Cfr10I RCCGGY 2 cut(s) 858, 1044
Cfr13I GGNCC 2 cut(s) 646, 1042
Cfr42I CCGCGG 1 cut(s) 939
Csp6I GTAC 2 cut(s) 712, 721
CviAII CATG 3 cut(s) 43, 736, 763
CviQI GTAC 2 cut(s) 712, 721
DdeI CTNAG 2 cut(s) 443, 830
DinI GGCGCC 1 cut(s) 241
DpnI GATC 9 cut(s) 188, 269, 308, 585, 828, 845, 875, 953, 1053
DpnII GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
EaeI YGGCCR 3 cut(s) 54, 222, 759
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
Ecl136II GAGCTC 1 cut(s) 442
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 243, 762
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 646, 1042
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 646
EcoT14I CCWWGG 2 cut(s) 243, 762
EcoT38I GRGCYC 1 cut(s) 444
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 243, 762
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 3 cut(s) 46, 739, 766
FaiI YATR 8 cut(s) 44, 260, 453, 681, 683, 737, 764, 968
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FatI CATG 3 cut(s) 42, 735, 762
FbaI TGATCA 2 cut(s) 306, 583
FblI GTMKAC 1 cut(s) 995
Fnu4HI GCNGC 7 cut(s) 54, 57, 69, 255, 633, 759, 936
FokI GGATG 2 cut(s) 95, 573
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 7 cut(s) 54, 57, 69, 255, 633, 759, 936
FspBI CTAG 1 cut(s) 519
GlaI GCGC 2 cut(s) 241, 569
GluI GCNGC 7 cut(s) 54, 57, 69, 255, 633, 759, 936
HaeII RGCGCY 2 cut(s) 243, 571
HaeIII GGCC 4 cut(s) 56, 195, 224, 761
HapII CCGG 6 cut(s) 113, 590, 620, 732, 859, 1045
HhaI GCGC 2 cut(s) 242, 570
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 3 cut(s) 46, 739, 766
Hin6I GCGC 2 cut(s) 240, 568
HinP1I GCGC 2 cut(s) 240, 568
HincII GTYRAC 2 cut(s) 277, 996
HindII GTYRAC 2 cut(s) 277, 996
HinfI GANTC 4 cut(s) 322, 338, 914, 987
HpaII CCGG 6 cut(s) 113, 590, 620, 732, 859, 1045
HphI GGTGA 3 cut(s) 160, 301, 883
Hpy166II GTNNAC 3 cut(s) 277, 996, 1026
Hpy188I TCNGA 6 cut(s) 306, 424, 826, 831, 848, 919
Hpy188III TCNNGA 2 cut(s) 217, 652
Hpy8I GTNNAC 3 cut(s) 277, 996, 1026
Hpy99I CGWCG 3 cut(s) 34, 194, 986
HpyAV CCTTC 3 cut(s) 142, 622, 828
HpyCH4III ACNGT 7 cut(s) 30, 172, 350, 395, 401, 420, 541
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 3 cut(s) 98, 819, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 74, 764
HpyF3I CTNAG 2 cut(s) 443, 830
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 3 cut(s) 46, 739, 766
HspAI GCGC 2 cut(s) 240, 568
KasI GGCGCC 1 cut(s) 239
Ksp22I TGATCA 2 cut(s) 306, 583
KspI CCGCGG 1 cut(s) 939
Kzo9I GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
LmnI GCTCC 2 cut(s) 575, 616
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 2 cut(s) 339, 966
MaeI CTAG 1 cut(s) 519
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 395, 837
MalI GATC 9 cut(s) 188, 269, 308, 585, 828, 845, 875, 953, 1053
MboI GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
MboII GAAGA 2 cut(s) 953, 1016
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 5 cut(s) 412, 514, 769, 820, 1014
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 1 cut(s) 981
MmeI TCCRAC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 254, 938
MspI CCGG 6 cut(s) 113, 590, 620, 732, 859, 1045
MspR9I CCNGG 2 cut(s) 113, 732
MvnI CGCG 1 cut(s) 938
MwoI GCNNNNNNNGC 3 cut(s) 65, 74, 764
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 2 cut(s) 113, 732
NcoI CCATGG 1 cut(s) 762
NdeII GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
NlaIII CATG 3 cut(s) 46, 739, 766
NlaIV GGNNCC 2 cut(s) 241, 618
NmeAIII GCCGAG 1 cut(s) 216
NotI GCGGCCGC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 600
PaeR7I CTCGAG 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 322, 338, 914
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 7 cut(s) 55, 58, 70, 256, 634, 760, 937
PleI GAGTC 1 cut(s) 981
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 981
PpuMI RGGWCCY 1 cut(s) 646
PshAI GACNNNNGTC 1 cut(s) 991
Psp124BI GAGCTC 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 646
PspN4I GGNNCC 2 cut(s) 241, 618
PspPI GGNCC 2 cut(s) 646, 1042
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 304
RsaI GTAC 2 cut(s) 713, 722
RsaNI GTAC 2 cut(s) 712, 721
SacI GAGCTC 1 cut(s) 444
SacII CCGCGG 1 cut(s) 939
SalI GTCGAC 1 cut(s) 994
SatI GCNGC 7 cut(s) 54, 57, 69, 255, 633, 759, 936
Sau3AI GATC 9 cut(s) 186, 267, 306, 583, 826, 843, 873, 951, 1051
Sau96I GGNCC 2 cut(s) 646, 1042
SchI GAGTC 1 cut(s) 981
ScrFI CCNGG 2 cut(s) 113, 732
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 2 cut(s) 339, 966
SfoI GGCGCC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 650
Sfr303I CCGCGG 1 cut(s) 939
SfuI TTCGAA 1 cut(s) 600
SgrBI CCGCGG 1 cut(s) 939
SinI GGWCC 2 cut(s) 646, 1042
SlaI CTCGAG 1 cut(s) 650
SmlI CTYRAG 2 cut(s) 650, 776
SmoI CTYRAG 2 cut(s) 650, 776
Sse9I AATT 5 cut(s) 412, 514, 769, 820, 1014
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 1 cut(s) 519
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 111, 730
StyI CCWWGG 2 cut(s) 243, 762
TaaI ACNGT 7 cut(s) 30, 172, 350, 395, 401, 420, 541
TaiI ACGT 1 cut(s) 131
TasI AATT 5 cut(s) 412, 514, 769, 820, 1014
TauI GCSGC 6 cut(s) 56, 59, 71, 635, 761, 938
TfiI GAWTC 3 cut(s) 322, 338, 914
TscAI CASTG 2 cut(s) 514, 821
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 641, 967
TspGWI ACGGA 3 cut(s) 572, 738, 999
TspRI CASTG 2 cut(s) 514, 821
VpaK11BI GGWCC 2 cut(s) 646, 1042
XapI RAATTY 2 cut(s) 412, 514
XhoI CTCGAG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.