MD08G1046900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015342
Physical Location & Seq
Reverse (-)
3538861 .. 3541877
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1046900.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGAGGCAGCTGAGTCGATCCTCAAACCATAAAACCAGACAATTAATGGAATTAGATAAACTCAGTAAGCTCAAAGAGGAGCCTCATCATCTCTCTGGTGCTTACATCCGTAGCCTAGTGAAACAACTCACCTCTTCAAGATCCACCTTGAACCCCAATAAGGACGTCGTCCTGAACTGTGACGGTATCCCAAGTCAAAACACTGTCAAGTTTGGCGATGATAATTTTAGCGAAACCCAGCAACACACCCAACAACCCCACCAGCCTCAACAACACAAAAAACAAGTGAGGAGGAGACTCCACACCAGCAGGCCCTACCAAGAGAGGCTTTTGAACATGGCTGAGGCTAGGAGAGAGATTGTCACTGCCCTCAAGTTTCATAGGGCAGCTATGAAACAAGCCACTGAACAACAGAACCAACAAGACCAACAACCTCAGGAGCAACAACCACTTGAGCCACAAATCCCTCGACCTCGCTTAGAACAAGAAGCGAGGATCAAATCGAGGAGAAACCCTCGAATCTACCTGTCAAGCGCGTCGAATTACCAGGAAACTCCTCCCTTCTCTTCTGATTTTTCTTACCAATATCCAAATCCATATCAATATTCAAATCCTTATTCTTGGACTCCTTCCACAATTGCACCTCCACCACCTTATGAAAATTTTGATTTCACACTTCCGAACCAAACCCTTGGCCTCAACCTCAACTTCCAAGATTTCAACAACATAAACACAACCCTTTACCACCACACCAAAAGCCCGTCAATTCACTCCGCCTCCACCTCATCTCCCTCGTCATCTTCTTCCCCCACTCTCTCTGCTACTACTACCGATCCTGAAATTCTGACCTCAGCTGCAGGAGCTTCTTTCACTTCCCATATTGAAGCGGAGGATGCTTCTGCGGTGGCTGATGCTGTTGACTCCGCCGGGGTTATCACAATCAGTGGCGGAGATGGCATGCATGCAGCAATGGACGACAAAGAGATGGCGGAGATCAGATCGATAGGAGAGCAGCACCAGATGGAGTGGAACGACACCATGAACTTGGTCACGTCAGCATGGTGGTTTAAATTCTTGAAGGCCATGGAGCTCGGTGGGGGCCCGCAAGTGAAGGATGAGGACGATGATGATGGATACCACCATCCGTTTGATGAAGTCATGGAATTTCCAGCCTGGTTGAACGCAAGTGAAAGCGGATTTCAGAATGATCATTTGAATGATTGCTACTCAGACGATTATTTTCAAGATCCTGCGTTGCCTTGCATGGACATTGGAGAAATTGAAGGTATTGATGCGGAGTGGCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

437

Amino Acids

49.38

Weight (kDa)

5.14

Isoelectric Point (pI)

58.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21280 AT5G21280
fragaria_vesca FvH4_2g37040
malus_domestica MD08G1046900.v1.1 MD15G1047100.v1.1
prunus_persica Prupe.1G401300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304741
rosa_laevigata RLG00000010954
rosa_multiflora Rmu_ssc0000139.1_g000010
rosa_roxburghii Rroxscaffold_7G00163540
rosa_rugosa Rorug06G0334100
rosa_samantha Rh6AG446000 Rh6BG455600 Rh6CG459400 Rh6DG446300
rosa_wichuraiana Rw6G038800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccII CGCG 1 cut(s) 536
AciI CCGC 9 cut(s) 774, 887, 902, 924, 948, 989, 1103, 1194, 1295
AclWI GGATC 5 cut(s) 12, 135, 503, 827, 1241
AcsI RAATTY 4 cut(s) 661, 840, 1070, 1163
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 160
AjiI CACGTC 1 cut(s) 1053
AjnI CCWGG 2 cut(s) 546, 1172
AluBI AGCT 6 cut(s) 10, 70, 389, 854, 863, 1090
AluI AGCT 6 cut(s) 10, 70, 389, 854, 863, 1090
Alw21I GWGCWC 1 cut(s) 1092
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 5 cut(s) 12, 135, 503, 827, 1241
AoxI GGCC 4 cut(s) 311, 694, 1080, 1099
ApaI GGGCCC 1 cut(s) 1103
ApeKI GCWGC 5 cut(s) 7, 386, 854, 965, 1012
ApoI RAATTY 4 cut(s) 661, 840, 1070, 1163
AseI ATTAAT 1 cut(s) 44
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 3 cut(s) 312, 1099, 1100
AsuC2I CCSGG 1 cut(s) 928
AsuHPI GGTGA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 1303
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 1103
BanII GRGCYC 2 cut(s) 1092, 1103
Bbv12I GWGCWC 1 cut(s) 1092
BbvCI CCTCAGC 2 cut(s) 342, 850
BbvI GCAGC 5 cut(s) 19, 398, 841, 977, 1024
BccI CCATC 5 cut(s) 947, 979, 1015, 1124, 1149
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 548, 1174
BciVI GTATCC 2 cut(s) 197, 1127
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 928
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 3 cut(s) 116, 348, 1304
BfmI CTRYAG 1 cut(s) 855
BfuI GTATCC 2 cut(s) 197, 1127
BisI GCNGC 5 cut(s) 8, 387, 855, 966, 1013
BlsI GCNGC 5 cut(s) 9, 388, 856, 967, 1014
Bme1390I CCNGG 3 cut(s) 548, 928, 1174
BmgBI CACGTC 1 cut(s) 1053
BmgT120I GGNCC 3 cut(s) 312, 1099, 1100
BmiI GGNNCC 3 cut(s) 81, 1100, 1101
BmrFI CCNGG 3 cut(s) 548, 928, 1174
BmsI GCATC 3 cut(s) 883, 901, 1282
BmtI GCTAGC 1 cut(s) 1307
BplI GAGNNNNNCTC 2 cut(s) 499, 531
Bpu10I CCTNAGC 2 cut(s) 342, 850
BpuEI CTTGAG 2 cut(s) 356, 473
BpuMI CCSGG 1 cut(s) 928
Bsa29I ATCGAT 1 cut(s) 1001
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 691, 927, 1083
BsaXI ACNNNNNCTCC 4 cut(s) 761, 772, 791, 802
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse21I CCTNAGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 2 cut(s) 548, 1174
BseCI ATCGAT 1 cut(s) 1001
BseDI CCNNGG 3 cut(s) 691, 927, 1083
BseGI GGATG 4 cut(s) 105, 898, 1120, 1141
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 5 cut(s) 76, 333, 449, 864, 1242
BseRI GAGGAG 5 cut(s) 92, 304, 307, 520, 546
BseSI GKGCMC 1 cut(s) 1103
BseXI GCAGC 5 cut(s) 19, 398, 841, 977, 1024
BseYI CCCAGC 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 4 cut(s) 313, 696, 1082, 1101
BshVI ATCGAT 1 cut(s) 1001
BsiHKAI GWGCWC 1 cut(s) 1092
BsiSI CCGG 1 cut(s) 927
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 289
BsnI GGCC 4 cut(s) 313, 696, 1082, 1101
Bsp120I GGGCCC 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 1092, 1103
Bsp143I GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
Bsp19I CCATGG 1 cut(s) 1083
BspACI CCGC 9 cut(s) 774, 887, 902, 924, 948, 989, 1103, 1194, 1295
BspANI GGCC 4 cut(s) 313, 696, 1082, 1101
BspCNI CTCAG 5 cut(s) 75, 334, 448, 863, 1241
BspDI ATCGAT 1 cut(s) 1001
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 3 cut(s) 81, 1100, 1101
BspMAI CTGCAG 1 cut(s) 859
BspOI GCTAGC 1 cut(s) 1307
BspPI GGATC 5 cut(s) 12, 135, 503, 827, 1241
BsrDI GCAATG 1 cut(s) 975
BssECI CCNNGG 3 cut(s) 691, 927, 1083
BssMI GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 2 cut(s) 691, 1083
Bst2UI CCWGG 2 cut(s) 548, 1174
Bst4CI ACNGT 3 cut(s) 179, 185, 205
Bst6I CTCTTC 2 cut(s) 139, 571
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 5 cut(s) 311, 959, 963, 1103, 1305
BstDEI CTNAG 7 cut(s) 11, 62, 342, 435, 478, 850, 1228
BstDSI CCRYGG 1 cut(s) 1083
BstF5I GGATG 4 cut(s) 105, 898, 1120, 1141
BstFNI CGCG 1 cut(s) 536
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 8 cut(s) 20, 143, 498, 835, 996, 1001, 1210, 1249
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
BstMWI GCNNNNNNNGC 3 cut(s) 860, 893, 954
BstNI CCWGG 2 cut(s) 548, 1174
BstNSI RCATGY 2 cut(s) 961, 965
BstSCI CCNGG 3 cut(s) 546, 926, 1172
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 1103
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 5 cut(s) 19, 398, 841, 977, 1024
BstX2I RGATCY 2 cut(s) 140, 1246
BstXI CCANNNNNNTGG 2 cut(s) 692, 1045
BstYI RGATCY 2 cut(s) 140, 1246
Bsu15I ATCGAT 1 cut(s) 1001
Bsu36I CCTNAGG 1 cut(s) 435
BsuI GTATCC 2 cut(s) 197, 1127
BsuRI GGCC 4 cut(s) 313, 696, 1082, 1101
BsuTUI ATCGAT 1 cut(s) 1001
BtgI CCRYGG 1 cut(s) 1083
BtgZI GCGATG 1 cut(s) 231
BtrI CACGTC 1 cut(s) 1053
BtsCI GGATG 4 cut(s) 105, 898, 1120, 1141
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 4 cut(s) 201, 363, 402, 949
Cac8I GCNNGC 5 cut(s) 311, 959, 963, 1103, 1305
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 3 cut(s) 312, 1099, 1100
ClaI ATCGAT 1 cut(s) 1001
CseI GACGC 1 cut(s) 525
CviAII CATG 8 cut(s) 337, 958, 962, 1039, 1059, 1084, 1159, 1264
DdeI CTNAG 7 cut(s) 11, 62, 342, 435, 478, 850, 1228
DpnI GATC 8 cut(s) 19, 142, 497, 834, 995, 1000, 1209, 1248
DpnII GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
DraI TTTAAA 1 cut(s) 1069
Eam1104I CTCTTC 2 cut(s) 139, 571
EarI CTCTTC 2 cut(s) 139, 571
EciI GGCGGA 4 cut(s) 763, 913, 963, 1004
Ecl136II GAGCTC 1 cut(s) 1090
Eco130I CCWWGG 2 cut(s) 691, 1083
Eco24I GRGCYC 2 cut(s) 1092, 1103
Eco53kI GAGCTC 1 cut(s) 1090
Eco81I CCTNAGG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 1090
EcoO109I RGGNCCY 2 cut(s) 312, 1099
EcoRII CCWGG 2 cut(s) 546, 1172
EcoT14I CCWWGG 2 cut(s) 691, 1083
EcoT22I ATGCAT 1 cut(s) 963
EcoT38I GRGCYC 2 cut(s) 1092, 1103
ErhI CCWWGG 2 cut(s) 691, 1083
FaeI CATG 8 cut(s) 340, 961, 965, 1042, 1062, 1087, 1162, 1267
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 8 cut(s) 336, 957, 961, 1038, 1058, 1083, 1158, 1263
FauI CCCGC 1 cut(s) 1110
FbaI TGATCA 1 cut(s) 1207
Fnu4HI GCNGC 5 cut(s) 8, 387, 855, 966, 1013
FokI GGATG 4 cut(s) 92, 905, 1127, 1128
FriOI GRGCYC 2 cut(s) 1092, 1103
Fsp4HI GCNGC 5 cut(s) 8, 387, 855, 966, 1013
FspBI CTAG 3 cut(s) 116, 348, 1304
GlaI GCGC 1 cut(s) 535
GluI GCNGC 5 cut(s) 8, 387, 855, 966, 1013
GsaI CCCAGC 1 cut(s) 241
HaeIII GGCC 4 cut(s) 313, 696, 1082, 1101
HapII CCGG 1 cut(s) 927
HgaI GACGC 1 cut(s) 525
HhaI GCGC 1 cut(s) 536
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 8 cut(s) 340, 961, 965, 1042, 1062, 1087, 1162, 1267
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HinfI GANTC 5 cut(s) 13, 297, 519, 625, 920
HpaII CCGG 1 cut(s) 927
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 919
Hpy188I TCNGA 6 cut(s) 571, 681, 846, 998, 1203, 1231
Hpy188III TCNNGA 6 cut(s) 138, 172, 437, 836, 1075, 1244
Hpy8I GTNNAC 1 cut(s) 919
Hpy99I CGWCG 2 cut(s) 170, 541
HpyAV CCTTC 5 cut(s) 571, 639, 1072, 1105, 1277
HpyCH4III ACNGT 3 cut(s) 179, 185, 205
HpyCH4IV ACGT 2 cut(s) 165, 1052
HpyCH4V TGCA 5 cut(s) 641, 857, 961, 965, 1263
HpyF10VI GCNNNNNNNGC 3 cut(s) 860, 893, 954
HpyF3I CTNAG 7 cut(s) 11, 62, 342, 435, 478, 850, 1228
HpySE526I ACGT 2 cut(s) 165, 1052
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 8 cut(s) 340, 961, 965, 1042, 1062, 1087, 1162, 1267
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 1207
Kzo9I GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
LmnI GCTCC 4 cut(s) 79, 439, 860, 1087
Lsp1109I GCAGC 5 cut(s) 19, 398, 841, 977, 1024
LweI GCATC 3 cut(s) 883, 901, 1282
MaeI CTAG 3 cut(s) 116, 348, 1304
MaeII ACGT 2 cut(s) 165, 1052
MaeIII GTNAC 3 cut(s) 179, 361, 1048
MalI GATC 8 cut(s) 19, 142, 497, 834, 995, 1000, 1209, 1248
MboI GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
MboII GAAGA 4 cut(s) 126, 558, 792, 795
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 2 cut(s) 140, 1246
MhlI GDGCHC 2 cut(s) 1092, 1103
MlyI GAGTC 4 cut(s) 22, 291, 619, 914
Mph1103I ATGCAT 1 cut(s) 963
MseI TTAA 2 cut(s) 44, 1068
MslI CAYNNNNRTG 1 cut(s) 1215
MspA1I CMGCKG 2 cut(s) 10, 854
MspI CCGG 1 cut(s) 927
MspR9I CCNGG 3 cut(s) 548, 928, 1174
MunI CAATTG 1 cut(s) 636
MvaI CCWGG 2 cut(s) 548, 1174
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 3 cut(s) 860, 893, 954
NciI CCSGG 1 cut(s) 928
NcoI CCATGG 1 cut(s) 1083
NdeII GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
NheI GCTAGC 1 cut(s) 1303
NlaIII CATG 8 cut(s) 340, 961, 965, 1042, 1062, 1087, 1162, 1267
NlaIV GGNNCC 3 cut(s) 81, 1100, 1101
NmuCI GTSAC 3 cut(s) 179, 361, 1048
NsiI ATGCAT 1 cut(s) 963
NspI RCATGY 2 cut(s) 961, 965
PaeI GCATGC 2 cut(s) 961, 965
PfeI GAWTC 1 cut(s) 519
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 5 cut(s) 9, 388, 856, 967, 1014
PleI GAGTC 4 cut(s) 21, 291, 619, 914
PpsI GAGTC 4 cut(s) 21, 291, 619, 914
PshBI ATTAAT 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 1092
Psp6I CCWGG 2 cut(s) 546, 1172
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 2 cut(s) 546, 1172
PspN4I GGNNCC 3 cut(s) 81, 1100, 1101
PspOMI GGGCCC 1 cut(s) 1099
PspPI GGNCC 3 cut(s) 312, 1099, 1100
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 2 cut(s) 140, 1246
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 2 cut(s) 10, 854
RseI CAYNNNNRTG 1 cut(s) 1215
SacI GAGCTC 1 cut(s) 1092
SaqAI TTAA 2 cut(s) 44, 1068
SatI GCNGC 5 cut(s) 8, 387, 855, 966, 1013
Sau3AI GATC 8 cut(s) 17, 140, 495, 832, 993, 998, 1207, 1246
Sau96I GGNCC 3 cut(s) 312, 1099, 1100
SchI GAGTC 4 cut(s) 22, 291, 619, 914
ScrFI CCNGG 3 cut(s) 548, 928, 1174
SduI GDGCHC 2 cut(s) 1092, 1103
SfaNI GCATC 3 cut(s) 883, 901, 1282
SfcI CTRYAG 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 1215
SmlI CTYRAG 2 cut(s) 371, 452
SmoI CTYRAG 2 cut(s) 371, 452
SphI GCATGC 2 cut(s) 961, 965
SsiI CCGC 9 cut(s) 774, 887, 902, 924, 948, 989, 1103, 1194, 1295
SspI AATATT 1 cut(s) 605
SspMI CTAG 3 cut(s) 116, 348, 1304
SstI GAGCTC 1 cut(s) 1092
StyD4I CCNGG 3 cut(s) 546, 926, 1172
StyI CCWWGG 2 cut(s) 691, 1083
TaaI ACNGT 3 cut(s) 179, 185, 205
TaiI ACGT 2 cut(s) 168, 1055
TaqI TCGA 6 cut(s) 16, 469, 503, 517, 539, 1001
TfiI GAWTC 1 cut(s) 519
Tru1I TTAA 2 cut(s) 44, 1068
Tru9I TTAA 2 cut(s) 44, 1068
TscAI CASTG 4 cut(s) 208, 370, 409, 949
TseFI GTSAC 3 cut(s) 179, 361, 1048
TseI GCWGC 5 cut(s) 7, 386, 854, 965, 1012
Tsp45I GTSAC 3 cut(s) 179, 361, 1048
TspDTI ATGAA 5 cut(s) 368, 407, 672, 1055, 1167
TspGWI ACGGA 2 cut(s) 98, 1134
TspRI CASTG 4 cut(s) 208, 370, 409, 949
Tth111I GACNNNGTC 1 cut(s) 167
VspI ATTAAT 1 cut(s) 44
XapI RAATTY 4 cut(s) 661, 840, 1070, 1163
XceI RCATGY 2 cut(s) 961, 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XspI CTAG 3 cut(s) 116, 348, 1304
ZraI GACGTC 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.