MD15G1047100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015342
Physical Location & Seq
Forward (+)
3240794 .. 3243540
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1047100.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGAATTAGATAAACGCAGTAAGCTCAAAGAGGAGCCTCATAATCTCTCTGGTGCTTACATCCGTAGCCTAATGAAACAACTCACCTCTTCAAGAACCAAAGAGCCCATGAATCCCAACCAGGACATCGCACTGGGCTGCGATGGTATCTCAAGCCACAACACTGCCAAATTTGGCAACAAAAAATTTGGAGAAACCCAGAAACCCCACCAGCCTCAACAACACAAAAAACAAGTGAGGCGGAGACTCCACACCAGCAGGCCCTACCAAGAGAGGCTTTTGAACATGGCTGAGGCTAGGAGAGAGATTGTTACTGCTCTCAAGTTTCATAGGGCAGCTATGAAACAAGCCAGTGAACAACAGCAACAACAAGACCAACAAGCACTTGAGCCACAATCCCGACCTCGCTCCGAACAAGAAGCAAGGATCGAATTGAGGAGAAACCCTGGGATTTACCCGTCAAGCACGTCAAATTATCAGGAAACTCCGCCCTTCTCATCTGATTTTTCATTCCAATATCCAAACCCAAGTCAATATTCAAATCCATATTCTTGGACTCATTCCACAATTGCACCTCCACTACCTTACGAAAATTTTGATTTCACACTTCCAAACCAAACCCTTGGCCTCAACCTCAACTTCCAAGATTTCAACAACATAAACACAACCCTTTACCACAACACCAACAGCCCGTCAATTTTCTCCACCTCCGCCTCGTCTCCCTCGTCTTCTTCTTCCCCCACTCTCTCTGCTGCCACTACCGATCCTGAAATTCTCTCAGCTGCAGGAGCCTCTTCCTCTTTCCATATTGAGGCGGAGGATGCTCCTGCGGTGGCTGATGCTGTTGACTCCGCTGGGGTTATCACAATCAGTGGCGGAGATGGCATGCATGCAGCAATCGACGACGAGGAGATGGCGGAGATCAGATCGATAGGGGAGCAGCACCAGATGGAGTGGAACGACACCATGAACTTGGTCACGTCAGCATGGTGGTTTAAGTTTTTGAAGGCTGTGGAGCTCCGTGGGGGCCCACAAGTGAAGGATGAGGACGATGGCGATGGATACCACCATCCGTTTGATGAAGTCATGGAACTTCCAGCCTGGTTGAATGCAAATGAGAGCTGTTTTCAGAATGATCATTTGAATGCTTGCTACTCAGGCGACTATTTTCAAGATCCTGCCTTGCCTTGCATGGACATTGGAGAAATTGAAGGTGTTGACGGGGAGTGGTTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

413

Amino Acids

46.15

Weight (kDa)

5.0

Isoelectric Point (pI)

66.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21280 AT5G21280
fragaria_vesca FvH4_2g37040
malus_domestica MD08G1046900.v1.1 MD15G1047100.v1.1
prunus_persica Prupe.1G401300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304741
rosa_laevigata RLG00000010954
rosa_multiflora Rmu_ssc0000139.1_g000010
rosa_roxburghii Rroxscaffold_7G00163540
rosa_rugosa Rorug06G0334100
rosa_samantha Rh6AG446000 Rh6BG455600 Rh6CG459400 Rh6DG446300
rosa_wichuraiana Rw6G038800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 241, 488, 711, 815, 830, 852, 876, 917
AclWI GGATC 3 cut(s) 434, 758, 1169
AcsI RAATTY 4 cut(s) 170, 185, 592, 771
AfiI CCNNNNNNNGG 1 cut(s) 811
AgsI TTSAA 9 cut(s) 93, 283, 540, 652, 1006, 1108, 1144, 1172, 1211
AjiI CACGTC 2 cut(s) 468, 981
AjnI CCWGG 3 cut(s) 120, 445, 1100
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 5 cut(s) 25, 338, 782, 1018, 1122
AluI AGCT 5 cut(s) 25, 338, 782, 1018, 1122
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 2 cut(s) 238, 723
AlwI GGATC 3 cut(s) 434, 758, 1169
AoxI GGCC 3 cut(s) 260, 625, 1027
ApaI GGGCCC 1 cut(s) 1031
ApeKI GCWGC 6 cut(s) 138, 335, 752, 782, 893, 940
ApoI RAATTY 4 cut(s) 170, 185, 592, 771
AspS9I GGNCC 3 cut(s) 261, 1027, 1028
AsuHPI GGTGA 1 cut(s) 76
BaeGI GKGCMC 1 cut(s) 1031
BanII GRGCYC 3 cut(s) 108, 1020, 1031
BbsI GAAGAC 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 1020
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 6 cut(s) 125, 347, 739, 769, 905, 952
BccI CCATC 7 cut(s) 137, 875, 907, 943, 1046, 1052, 1077
BciT130I CCWGG 3 cut(s) 122, 447, 1102
BciVI GTATCC 1 cut(s) 1055
BclI TGATCA 1 cut(s) 1135
BcoDI GTCTC 2 cut(s) 238, 723
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 783
BfuI GTATCC 1 cut(s) 1055
BisI GCNGC 6 cut(s) 139, 336, 753, 783, 894, 941
BlsI GCNGC 6 cut(s) 140, 337, 754, 784, 895, 942
Bme1390I CCNGG 3 cut(s) 122, 447, 1102
BmgBI CACGTC 2 cut(s) 468, 981
BmgT120I GGNCC 3 cut(s) 261, 1027, 1028
BmiI GGNNCC 4 cut(s) 36, 790, 1028, 1029
BmrFI CCNGG 3 cut(s) 122, 447, 1102
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 811, 829
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 3 cut(s) 136, 305, 407
Bsa29I ATCGAT 1 cut(s) 929
BsaJI CCNNGG 4 cut(s) 445, 446, 622, 1021
Bsc4I CCNNNNNNNGG 1 cut(s) 811
Bse1I ACTGG 2 cut(s) 138, 351
BseBI CCWGG 3 cut(s) 122, 447, 1102
BseCI ATCGAT 1 cut(s) 929
BseDI CCNNGG 4 cut(s) 445, 446, 622, 1021
BseGI GGATG 4 cut(s) 60, 826, 1048, 1069
BseLI CCNNNNNNNGG 1 cut(s) 811
BseMII CTCAG 3 cut(s) 282, 792, 1170
BseNI ACTGG 2 cut(s) 138, 351
BseRI GAGGAG 3 cut(s) 47, 451, 923
BseSI GKGCMC 1 cut(s) 1031
BseXI GCAGC 6 cut(s) 125, 347, 739, 769, 905, 952
BseYI CCCAGC 1 cut(s) 854
BshFI GGCC 3 cut(s) 262, 627, 1029
BshVI ATCGAT 1 cut(s) 929
BsiHKAI GWGCWC 1 cut(s) 1020
BslI CCNNNNNNNGG 1 cut(s) 811
BsmAI GTCTC 2 cut(s) 238, 723
BsmBI CGTCTC 1 cut(s) 723
BsmI GAATGC 2 cut(s) 1114, 1150
BsnI GGCC 3 cut(s) 262, 627, 1029
Bsp120I GGGCCC 1 cut(s) 1027
Bsp1286I GDGCHC 3 cut(s) 108, 1020, 1031
Bsp143I GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
BspACI CCGC 8 cut(s) 241, 488, 711, 815, 830, 852, 876, 917
BspANI GGCC 3 cut(s) 262, 627, 1029
BspCNI CTCAG 3 cut(s) 283, 791, 1169
BspDI ATCGAT 1 cut(s) 929
BspLI GGNNCC 4 cut(s) 36, 790, 1028, 1029
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 3 cut(s) 434, 758, 1169
BsrI ACTGG 2 cut(s) 138, 351
BssECI CCNNGG 4 cut(s) 445, 446, 622, 1021
BssMI GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 3 cut(s) 122, 447, 1102
Bst6I CTCTTC 2 cut(s) 94, 799
BstC8I GCNNGC 4 cut(s) 260, 887, 891, 1150
BstDEI CTNAG 3 cut(s) 291, 778, 1156
BstDSI CCRYGG 1 cut(s) 1021
BstF5I GGATG 4 cut(s) 60, 826, 1048, 1069
BstKTI GATC 6 cut(s) 429, 766, 924, 929, 1138, 1177
BstMAI GTCTC 2 cut(s) 238, 723
BstMBI GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
BstMWI GCNNNNNNNGC 4 cut(s) 788, 821, 882, 1158
BstNI CCWGG 3 cut(s) 122, 447, 1102
BstNSI RCATGY 2 cut(s) 889, 893
BstSCI CCNGG 3 cut(s) 120, 445, 1100
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 1031
BstV1I GCAGC 6 cut(s) 125, 347, 739, 769, 905, 952
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 1174
BstXI CCANNNNNNTGG 3 cut(s) 552, 623, 973
BstYI RGATCY 1 cut(s) 1174
Bsu15I ATCGAT 1 cut(s) 929
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 3 cut(s) 262, 627, 1029
BsuTUI ATCGAT 1 cut(s) 929
BtgI CCRYGG 1 cut(s) 1021
BtgZI GCGATG 3 cut(s) 112, 156, 1071
BtrI CACGTC 2 cut(s) 468, 981
BtsCI GGATG 4 cut(s) 60, 826, 1048, 1069
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 4 cut(s) 131, 162, 358, 877
Cac8I GCNNGC 4 cut(s) 260, 887, 891, 1150
Cfr13I GGNCC 3 cut(s) 261, 1027, 1028
ClaI ATCGAT 1 cut(s) 929
CviAII CATG 8 cut(s) 109, 286, 886, 890, 967, 987, 1087, 1192
DdeI CTNAG 3 cut(s) 291, 778, 1156
DpnI GATC 6 cut(s) 428, 765, 923, 928, 1137, 1176
DpnII GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
Eam1104I CTCTTC 2 cut(s) 94, 799
EarI CTCTTC 2 cut(s) 94, 799
EciI GGCGGA 6 cut(s) 256, 477, 700, 830, 891, 932
Ecl136II GAGCTC 1 cut(s) 1018
Eco130I CCWWGG 1 cut(s) 622
Eco24I GRGCYC 3 cut(s) 108, 1020, 1031
Eco53kI GAGCTC 1 cut(s) 1018
EcoICRI GAGCTC 1 cut(s) 1018
EcoO109I RGGNCCY 2 cut(s) 261, 1027
EcoRII CCWGG 3 cut(s) 120, 445, 1100
EcoT14I CCWWGG 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 891
EcoT38I GRGCYC 3 cut(s) 108, 1020, 1031
ErhI CCWWGG 1 cut(s) 622
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 8 cut(s) 112, 289, 889, 893, 970, 990, 1090, 1195
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FatI CATG 8 cut(s) 108, 285, 885, 889, 966, 986, 1086, 1191
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 6 cut(s) 139, 336, 753, 783, 894, 941
FokI GGATG 4 cut(s) 47, 833, 1055, 1056
FriOI GRGCYC 3 cut(s) 108, 1020, 1031
Fsp4HI GCNGC 6 cut(s) 139, 336, 753, 783, 894, 941
FspBI CTAG 1 cut(s) 297
GluI GCNGC 6 cut(s) 139, 336, 753, 783, 894, 941
GsaI CCCAGC 1 cut(s) 858
HaeIII GGCC 3 cut(s) 262, 627, 1029
Hin1II CATG 8 cut(s) 112, 289, 889, 893, 970, 990, 1090, 1195
HincII GTYRAC 2 cut(s) 847, 1219
HindII GTYRAC 2 cut(s) 847, 1219
HinfI GANTC 4 cut(s) 112, 246, 556, 848
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 356, 847, 1219
Hpy188I TCNGA 4 cut(s) 412, 502, 926, 1131
Hpy188III TCNNGA 5 cut(s) 93, 399, 479, 767, 1172
Hpy8I GTNNAC 3 cut(s) 356, 847, 1219
Hpy99I CGWCG 2 cut(s) 905, 908
HpyAV CCTTC 4 cut(s) 502, 1000, 1033, 1205
HpyCH4IV ACGT 2 cut(s) 467, 980
HpyCH4V TGCA 6 cut(s) 572, 785, 889, 893, 1112, 1191
HpyF10VI GCNNNNNNNGC 4 cut(s) 788, 821, 882, 1158
HpyF3I CTNAG 3 cut(s) 291, 778, 1156
HpySE526I ACGT 2 cut(s) 467, 980
Hsp92II CATG 8 cut(s) 112, 289, 889, 893, 970, 990, 1090, 1195
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
LmnI GCTCC 7 cut(s) 34, 413, 788, 829, 937, 1015, 1023
Lsp1109I GCAGC 6 cut(s) 125, 347, 739, 769, 905, 952
LweI GCATC 2 cut(s) 811, 829
MaeI CTAG 1 cut(s) 297
MaeII ACGT 2 cut(s) 467, 980
MaeIII GTNAC 2 cut(s) 310, 976
MalI GATC 6 cut(s) 428, 765, 923, 928, 1137, 1176
MboI GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
MboII GAAGA 5 cut(s) 81, 720, 723, 726, 786
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 1 cut(s) 1174
MhlI GDGCHC 3 cut(s) 108, 1020, 1031
MlyI GAGTC 3 cut(s) 240, 550, 842
Mph1103I ATGCAT 1 cut(s) 891
MseI TTAA 1 cut(s) 996
MslI CAYNNNNRTG 1 cut(s) 1143
MspA1I CMGCKG 2 cut(s) 782, 854
MspR9I CCNGG 3 cut(s) 122, 447, 1102
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 2 cut(s) 1114, 1150
MvaI CCWGG 3 cut(s) 122, 447, 1102
MwoI GCNNNNNNNGC 4 cut(s) 788, 821, 882, 1158
NdeII GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
NlaIII CATG 8 cut(s) 112, 289, 889, 893, 970, 990, 1090, 1195
NlaIV GGNNCC 4 cut(s) 36, 790, 1028, 1029
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 891
NspI RCATGY 2 cut(s) 889, 893
PaeI GCATGC 2 cut(s) 889, 893
PasI CCCWGGG 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 722
PctI GAATGC 2 cut(s) 1114, 1150
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 6 cut(s) 140, 337, 754, 784, 895, 942
PleI GAGTC 3 cut(s) 240, 550, 842
PpsI GAGTC 3 cut(s) 240, 550, 842
Psp124BI GAGCTC 1 cut(s) 1020
Psp6I CCWGG 3 cut(s) 120, 445, 1100
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 3 cut(s) 120, 445, 1100
PspN4I GGNNCC 4 cut(s) 36, 790, 1028, 1029
PspOMI GGGCCC 1 cut(s) 1027
PspPI GGNCC 3 cut(s) 261, 1027, 1028
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 782
RseI CAYNNNNRTG 1 cut(s) 1143
SacI GAGCTC 1 cut(s) 1020
SaqAI TTAA 1 cut(s) 996
SatI GCNGC 6 cut(s) 139, 336, 753, 783, 894, 941
Sau3AI GATC 6 cut(s) 426, 763, 921, 926, 1135, 1174
Sau96I GGNCC 3 cut(s) 261, 1027, 1028
SchI GAGTC 3 cut(s) 240, 550, 842
ScrFI CCNGG 3 cut(s) 122, 447, 1102
SduI GDGCHC 3 cut(s) 108, 1020, 1031
SfaNI GCATC 2 cut(s) 811, 829
SfcI CTRYAG 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 1143
SmlI CTYRAG 3 cut(s) 151, 320, 386
SmoI CTYRAG 3 cut(s) 151, 320, 386
SphI GCATGC 2 cut(s) 889, 893
SsiI CCGC 8 cut(s) 241, 488, 711, 815, 830, 852, 876, 917
SspI AATATT 1 cut(s) 536
SspMI CTAG 1 cut(s) 297
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 3 cut(s) 120, 445, 1100
StyI CCWWGG 1 cut(s) 622
TaiI ACGT 2 cut(s) 470, 983
TaqI TCGA 3 cut(s) 429, 900, 929
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TscAI CASTG 4 cut(s) 138, 169, 358, 877
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 6 cut(s) 138, 335, 752, 782, 893, 940
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 7 cut(s) 89, 125, 317, 356, 498, 983, 1095
TspGWI ACGGA 3 cut(s) 53, 1010, 1062
TspRI CASTG 4 cut(s) 138, 169, 358, 877
XapI RAATTY 4 cut(s) 170, 185, 592, 771
XceI RCATGY 2 cut(s) 889, 893
XspI CTAG 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.