MD08G1050200.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008646
Physical Location & Seq
Reverse (-)
3891038 .. 3897068
6031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1050200.v1.1.491

Sequence Viewer

Length: 2217 bp
ATGAGGAATGTCTTTGCCGCGCTCAAGCGCCACCGCCATGTCACTCTGCTAACCCAAACTTTACGCCATGACAGAAACGTGGAGACCGTAATACGAAAATGCATACCATTGGGTTACAATAACTATGTTGAAATTAGATGGAAGCAAGAAAAATTGTTTCCGCATCAATTGTTACAACGGAATTCTATTTCCACCAGTGTGCATGGTGAAATGGCATCTGCAGAGTATGCAAAGTTGAGGAAGGAATCACTAGAGAGTGAATTCGGGCACGCTCTTGATGCAGGCTCCAAAAGTATTTCTGTTGTCTACCGTTTTGGTCCGTTTTTGGCATTGTATAGAGCTGCAATTATTTCATTTCATGTGGTGAAGCTCACAATCTGGCAATTCTTTGTCCATGACATAAAGAAACGCGCCGTCAAGTTTCGTGAAACTCTAATTCGCTTGGGACCTTTCTACATCAAGCTCGGGCAGGCTTTGAGCACCAGACCAGACATACTGCCAACTATATACTGCCAAGAGCTTGTTAAGTTACAGGATCAAATACCACCATTTCCAACACGTGTTGCAATTAAATCTATTGAATCTCAGCTAGGTGCCCCCATTTCAAAACTTTTTGCCGACATCAGCCCAGAGCCTATTGCAGCAGCATCCCTAGGGCAGGTCTATAAAGCTCACTTGCACTCTGGGGAGCTGGTAGCAGTTAAAGTACAAAGGCCGGGTATGTCACTTGTATTGACCCTTGACGCCTTGTTGTTCAGTATGATTGGGGGACAGTTAAAGCGTTTTGCCAATGCCCGCAAAGATCTACTAGTGGCCGTGAATGAGATGGTGAGGCATATGTTTGATGAAATTGATTACATCCAAGAGGCAAAGAATGCTGACCGTTTTGCCTCTCTCTATGGTTCAGACCCAAGTGACGGGAAAAAGACCGGTCCAAAGCCTACAGCTGGAAAAAGTCTTAAACATAATGAGGCGAACTGTATAAAAGTTCCAAAAATATATTGGGATTTTACCCGTAGGGGTGTGCTTACAATGGAGTGGATTGATGGAATTAAGCTTACAGATGAAATTGGCCTAGAGAGGGCTGGTTTGAACAGAAAAGGATTAATTGACCAGGGATTATACTGCTCTTTGAGACAATTGCTTGAGGTGGGATTTTTCCATGCTGACCCACATCCGGGTAACCTTGTCGCTACTGATACTGGTGCTCTTGCATATTTTGATTTTGGAATGATGGGTGAAATTCCTCGACATTATCGTGTGGGACTTATTCAAGTGCTTGTGCATTTTGTTAATCGAGACTCCCTGGGTTTGGCAAATGACTTTCTTTCATTGGGATTCCTTCCTGAAGTGGTTGATATACAATTAGTTTCGGATGCATTGCAAGCATCCTTTAGCGACCGGACTAGACAGTCTCAAGATTTCCAGGGAGTAATGGACCAACTCTACGATGTTATGTACGAATTTAACTTCTCTCTTCCTCCGGACTATGCCCTGGTAATAAGAGCACTGGGATCACTAGAAGGCACAGCCAAGGTCCTTGATCCTAATTTTAAAGTTATTGAGAGCTCATATCCTTTTGTGATTGGAAGACTTCTGGCTGACCCAAATCCTGATATGAGAAGAATTTTAAGACAACTTCTTATACGCAACGATGGATCGATAAGGTGGAATAGGCTAGAACGCCTGATTGCAGCAATATCTGAACAGGCTTCTGAGAGTGCTGAGGAGTCCCCAAATTCTGCGGGCGGTACTCCAAATCCGTTGAGAAGTAAATCATTTGACATGCATTCAGTTGTTGCCGCCACTGAAGATCTTTTCCACTTCATTCTCTCTAAGAAGGGTGAGAGGGTGCGTGTATTCCTTGTTCGGGATATACTTGCTGCAGCTGATGCTTTTCTTGAGGATGAAGTTGTTGGTCGTATGTTCAATGAGAAGCCCGAATCCAGAGGCTCAGTTGAGTCAGAGGCACACACCATGGTCATGAGGGTTGTAAATGGGTTTCAGTATCTCCGTCAAGCTATAAAGTTGGCCCCGGAGGTGTGGACGGAAATGCTTATACGCATGGCAGTTACGCCCGAGGTTCATAAGTTTACATTAGACATCGTTTCGTCGCTAATCATTCATTTTAACGGCAAAATTCCAGAAACTACGTTTGTTTGTATGTCTAGACTTATGCACAAGATGGAAAAAAACCGCAGCTCTTCCGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

739

Amino Acids

83.36

Weight (kDa)

8.85

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 176 - 439 1.6e-70 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1411
Acc36I ACCTGC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 593
AccI GTMKAC 1 cut(s) 306
AccII CGCG 2 cut(s) 20, 411
AccIII TCCGGA 1 cut(s) 1483
AciI CCGC 8 cut(s) 18, 34, 161, 796, 1745, 1749, 1803, 2197
AclWI GGATC 4 cut(s) 543, 1522, 1538, 1666
AcoI YGGCCR 1 cut(s) 813
AcsI RAATTY 7 cut(s) 181, 260, 1242, 1463, 1626, 1738, 2139
AcuI CTGAAG 2 cut(s) 1368, 1830
AcvI CACGTG 1 cut(s) 560
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 3 cut(s) 708, 1460, 1753
AfiI CCNNNNNNNGG 8 cut(s) 658, 917, 947, 1081, 1177, 1178, 1312, 1870
AflIII ACRYGT 2 cut(s) 557, 559
AgeI ACCGGT 1 cut(s) 929
AgsI TTSAA 6 cut(s) 131, 581, 606, 1093, 1274, 1930
AhlI ACTAGT 1 cut(s) 808
AjnI CCWGG 4 cut(s) 1113, 1305, 1425, 1494
AleI CACNNNNGTG 1 cut(s) 197
Alw21I GWGCWC 4 cut(s) 482, 1210, 1510, 1571
Alw26I GTCTC 4 cut(s) 77, 1129, 1293, 1419
AlwI GGATC 4 cut(s) 543, 1522, 1538, 1666
Ama87I CYCGRG 2 cut(s) 464, 2078
Aor13HI TCCGGA 1 cut(s) 1483
AoxI GGCC 4 cut(s) 713, 813, 1072, 2031
ApeKI GCWGC 7 cut(s) 341, 641, 644, 1694, 1883, 1886, 2199
ApoI RAATTY 7 cut(s) 181, 260, 1242, 1463, 1626, 1738, 2139
AseI ATTAAT 1 cut(s) 1106
AsiGI ACCGGT 1 cut(s) 929
AspA2I CCTAGG 1 cut(s) 652
AspLEI GCGC 3 cut(s) 22, 30, 413
AspS9I GGNCC 6 cut(s) 317, 446, 932, 1438, 1537, 2032
AsuC2I CCSGG 3 cut(s) 717, 1179, 2036
AsuHPI GGTGA 5 cut(s) 218, 376, 841, 1250, 1856
AvaI CYCGRG 2 cut(s) 464, 2078
AvaII GGWCC 5 cut(s) 317, 446, 932, 1438, 1537
AvrII CCTAGG 1 cut(s) 652
BaeGI GKGCMC 2 cut(s) 270, 598
BanI GGYRCC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 1571
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 1597
Bbv12I GWGCWC 4 cut(s) 482, 1210, 1510, 1571
BbvCI CCTCAGC 1 cut(s) 1725
BbvI GCAGC 7 cut(s) 328, 653, 656, 1706, 1870, 1898, 2211
BccI CCATC 6 cut(s) 132, 820, 1040, 1228, 1649, 2179
BceAI ACGGC 3 cut(s) 398, 800, 2149
BciT130I CCWGG 4 cut(s) 1115, 1307, 1427, 1496
BcnI CCSGG 3 cut(s) 717, 1179, 2036
BcoDI GTCTC 4 cut(s) 77, 1129, 1293, 1419
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 9 cut(s) 251, 590, 653, 809, 1076, 1407, 1520, 1679, 2169
BfmI CTRYAG 3 cut(s) 219, 942, 1884
BfoI RGCGCY 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 649
BglII AGATCT 2 cut(s) 802, 1813
BisI GCNGC 9 cut(s) 18, 342, 642, 645, 1695, 1803, 1884, 1887, 2200
BlnI CCTAGG 1 cut(s) 652
BlsI GCNGC 9 cut(s) 19, 343, 643, 646, 1696, 1804, 1885, 1888, 2201
Bme1390I CCNGG 7 cut(s) 717, 1115, 1179, 1307, 1427, 1496, 2036
Bme18I GGWCC 5 cut(s) 317, 446, 932, 1438, 1537
BmeT110I CYCGRG 2 cut(s) 464, 2078
BmgT120I GGNCC 6 cut(s) 317, 446, 932, 1438, 1537, 2032
BmiI GGNNCC 4 cut(s) 286, 447, 595, 2034
BmrFI CCNGG 7 cut(s) 717, 1115, 1179, 1307, 1427, 1496, 2036
BmrI ACTGGG 1 cut(s) 1520
BmsI GCATC 7 cut(s) 172, 224, 268, 656, 1366, 1397, 1882
BmuI ACTGGG 1 cut(s) 1520
BpiI GAAGAC 1 cut(s) 1597
Bpu10I CCTNAGC 1 cut(s) 1725
BpuEI CTTGAG 4 cut(s) 8, 1166, 1401, 1922
BpuMI CCSGG 3 cut(s) 717, 1179, 2036
Bsa29I ATCGAT 1 cut(s) 1661
BsaAI YACGTR 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 77
BsaWI WCCGGW 3 cut(s) 929, 1401, 1483
Bsc4I CCNNNNNNNGG 8 cut(s) 658, 917, 947, 1081, 1177, 1178, 1312, 1870
Bse118I RCCGGY 1 cut(s) 929
Bse1I ACTGG 3 cut(s) 195, 1207, 1515
Bse3DI GCAATG 1 cut(s) 1379
BseAI TCCGGA 1 cut(s) 1483
BseBI CCWGG 4 cut(s) 1115, 1307, 1427, 1496
BseCI ATCGAT 1 cut(s) 1661
BseGI GGATG 6 cut(s) 647, 858, 1174, 1381, 1388, 1912
BseLI CCNNNNNNNGG 8 cut(s) 658, 917, 947, 1081, 1177, 1178, 1312, 1870
BseMI GCAATG 1 cut(s) 1379
BseMII CTCAG 4 cut(s) 599, 1707, 1716, 1968
BseNI ACTGG 3 cut(s) 195, 1207, 1515
BseRI GAGGAG 1 cut(s) 1742
BseSI GKGCMC 2 cut(s) 270, 598
BseXI GCAGC 7 cut(s) 328, 653, 656, 1706, 1870, 1898, 2211
Bsh1236I CGCG 2 cut(s) 20, 411
Bsh1285I CGRYCG 1 cut(s) 1402
BshFI GGCC 4 cut(s) 715, 815, 1074, 2033
BshNI GGYRCC 1 cut(s) 593
BshTI ACCGGT 1 cut(s) 929
BshVI ATCGAT 1 cut(s) 1661
BsiEI CGRYCG 1 cut(s) 1402
BsiHKAI GWGCWC 4 cut(s) 482, 1210, 1510, 1571
BsiHKCI CYCGRG 2 cut(s) 464, 2078
BsiSI CCGG 6 cut(s) 716, 930, 1178, 1402, 1484, 2036
BslFI GGGAC 4 cut(s) 459, 783, 1278, 1717
BslI CCNNNNNNNGG 8 cut(s) 658, 917, 947, 1081, 1177, 1178, 1312, 1870
BsmAI GTCTC 4 cut(s) 77, 1129, 1293, 1419
BsmFI GGGAC 4 cut(s) 459, 783, 1278, 1717
BsmI GAATGC 2 cut(s) 880, 1789
BsnI GGCC 4 cut(s) 715, 815, 1074, 2033
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 2 cut(s) 464, 2078
Bsp1286I GDGCHC 6 cut(s) 270, 482, 598, 1210, 1510, 1571
Bsp13I TCCGGA 1 cut(s) 1483
Bsp143I GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
Bsp19I CCATGG 1 cut(s) 1977
BspACI CCGC 8 cut(s) 18, 34, 161, 796, 1745, 1749, 1803, 2197
BspANI GGCC 4 cut(s) 715, 815, 1074, 2033
BspCNI CTCAG 4 cut(s) 598, 1708, 1717, 1967
BspDI ATCGAT 1 cut(s) 1661
BspEI TCCGGA 1 cut(s) 1483
BspFNI CGCG 2 cut(s) 20, 411
BspHI TCATGA 1 cut(s) 1983
BspLI GGNNCC 4 cut(s) 286, 447, 595, 2034
BspMAI CTGCAG 2 cut(s) 223, 1888
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 4 cut(s) 543, 1522, 1538, 1666
BspQI GCTCTTC 1 cut(s) 2209
BspT107I GGYRCC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 1379
BsrFI RCCGGY 1 cut(s) 929
BsrI ACTGG 3 cut(s) 195, 1207, 1515
BssAI RCCGGY 1 cut(s) 929
BssMI GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
BssNI GRCGYC 1 cut(s) 744
BssT1I CCWWGG 3 cut(s) 652, 1533, 1977
Bst2UI CCWGG 4 cut(s) 1115, 1307, 1427, 1496
Bst4CI ACNGT 6 cut(s) 88, 311, 774, 884, 980, 1413
Bst6I CTCTTC 2 cut(s) 1482, 2209
BstACI GRCGYC 1 cut(s) 744
BstAPI GCANNNNNTGC 3 cut(s) 227, 875, 1892
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 6 cut(s) 270, 283, 471, 796, 1386, 1747
BstDEI CTNAG 5 cut(s) 585, 1716, 1725, 1836, 1954
BstDSI CCRYGG 1 cut(s) 1977
BstEII GGTNACC 1 cut(s) 1181
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 6 cut(s) 647, 858, 1174, 1381, 1388, 1912
BstFNI CGCG 2 cut(s) 20, 411
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 3 cut(s) 22, 30, 413
BstKTI GATC 6 cut(s) 538, 805, 1517, 1546, 1661, 1816
BstMAI GTCTC 4 cut(s) 77, 1129, 1293, 1419
BstMBI GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
BstMCI CGRYCG 1 cut(s) 1402
BstMWI GCNNNNNNNGC 5 cut(s) 227, 278, 875, 1385, 1892
BstNI CCWGG 4 cut(s) 1115, 1307, 1427, 1496
BstNSI RCATGY 1 cut(s) 1789
BstPI GGTNACC 1 cut(s) 1181
BstSCI CCNGG 7 cut(s) 715, 1113, 1177, 1305, 1425, 1494, 2034
BstSFI CTRYAG 3 cut(s) 219, 942, 1884
BstSLI GKGCMC 2 cut(s) 270, 598
BstUI CGCG 2 cut(s) 20, 411
BstV1I GCAGC 7 cut(s) 328, 653, 656, 1706, 1870, 1898, 2211
BstV2I GAAGAC 1 cut(s) 1597
BstX2I RGATCY 2 cut(s) 802, 1813
BstYI RGATCY 2 cut(s) 802, 1813
Bsu15I ATCGAT 1 cut(s) 1661
BsuRI GGCC 4 cut(s) 715, 815, 1074, 2033
BsuTUI ATCGAT 1 cut(s) 1661
BtgI CCRYGG 1 cut(s) 1977
BtsCI GGATG 6 cut(s) 647, 858, 1174, 1381, 1388, 1912
BtsIMutI CAGTG 3 cut(s) 202, 1508, 1806
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 6 cut(s) 270, 283, 471, 796, 1386, 1747
CciI TCATGA 1 cut(s) 1983
CfoI GCGC 3 cut(s) 22, 30, 413
Cfr10I RCCGGY 1 cut(s) 929
Cfr13I GGNCC 6 cut(s) 317, 446, 932, 1438, 1537, 2032
ClaI ATCGAT 1 cut(s) 1661
CseI GACGC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 707, 1459, 1752
CspAI ACCGGT 1 cut(s) 929
CviQI GTAC 3 cut(s) 707, 1459, 1752
DdeI CTNAG 5 cut(s) 585, 1716, 1725, 1836, 1954
DpnI GATC 6 cut(s) 537, 804, 1516, 1545, 1660, 1815
DpnII GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
DraI TTTAAA 1 cut(s) 1555
DrdI GACNNNNNNGTC 1 cut(s) 1411
DseDI GACNNNNNNGTC 1 cut(s) 1411
EaeI YGGCCR 1 cut(s) 813
Eam1104I CTCTTC 2 cut(s) 1482, 2209
EarI CTCTTC 2 cut(s) 1482, 2209
Ecl136II GAGCTC 1 cut(s) 1569
Eco130I CCWWGG 3 cut(s) 652, 1533, 1977
Eco24I GRGCYC 1 cut(s) 1571
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 5 cut(s) 317, 446, 932, 1438, 1537
Eco53kI GAGCTC 1 cut(s) 1569
Eco57I CTGAAG 2 cut(s) 1368, 1830
Eco72I CACGTG 1 cut(s) 560
Eco88I CYCGRG 2 cut(s) 464, 2078
Eco91I GGTNACC 1 cut(s) 1181
EcoICRI GAGCTC 1 cut(s) 1569
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 446, 1537
EcoO65I GGTNACC 1 cut(s) 1181
EcoRI GAATTC 2 cut(s) 181, 260
EcoRII CCWGG 4 cut(s) 1113, 1305, 1425, 1494
EcoT14I CCWWGG 3 cut(s) 652, 1533, 1977
EcoT22I ATGCAT 3 cut(s) 104, 1381, 1791
EcoT38I GRGCYC 1 cut(s) 1571
ErhI CCWWGG 3 cut(s) 652, 1533, 1977
FaqI GGGAC 4 cut(s) 459, 783, 1278, 1717
FauI CCCGC 2 cut(s) 803, 1738
FauNDI CATATG 1 cut(s) 837
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 9 cut(s) 18, 342, 642, 645, 1695, 1803, 1884, 1887, 2200
FokI GGATG 6 cut(s) 634, 845, 1161, 1375, 1388, 1919
FriOI GRGCYC 1 cut(s) 1571
Fsp4HI GCNGC 9 cut(s) 18, 342, 642, 645, 1695, 1803, 1884, 1887, 2200
FspBI CTAG 9 cut(s) 251, 590, 653, 809, 1076, 1407, 1520, 1679, 2169
GlaI GCGC 3 cut(s) 21, 29, 412
GluI GCNGC 9 cut(s) 18, 342, 642, 645, 1695, 1803, 1884, 1887, 2200
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 4 cut(s) 715, 815, 1074, 2033
HapII CCGG 6 cut(s) 716, 930, 1178, 1402, 1484, 2036
HgaI GACGC 1 cut(s) 752
HhaI GCGC 3 cut(s) 22, 30, 413
Hin1I GRCGYC 1 cut(s) 744
Hin6I GCGC 3 cut(s) 20, 28, 411
HinP1I GCGC 3 cut(s) 20, 28, 411
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 7 cut(s) 245, 581, 1301, 1338, 1730, 1943, 1961
HpaII CCGG 6 cut(s) 716, 930, 1178, 1402, 1484, 2036
HphI GGTGA 5 cut(s) 218, 376, 841, 1250, 1856
Hpy166II GTNNAC 3 cut(s) 307, 2046, 2094
Hpy188I TCNGA 6 cut(s) 907, 1375, 1705, 1717, 1966, 2209
Hpy8I GTNNAC 3 cut(s) 307, 2046, 2094
Hpy99I CGWCG 1 cut(s) 2116
HpyAV CCTTC 4 cut(s) 235, 1352, 1517, 1834
HpyCH4III ACNGT 6 cut(s) 88, 311, 774, 884, 980, 1413
HpyCH4IV ACGT 3 cut(s) 78, 559, 2153
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 278, 875, 1385, 1892
HpyF3I CTNAG 5 cut(s) 585, 1716, 1725, 1836, 1954
HpySE526I ACGT 3 cut(s) 78, 559, 2153
Hsp92I GRCGYC 1 cut(s) 744
HspAI GCGC 3 cut(s) 20, 28, 411
Kpn2I TCCGGA 1 cut(s) 1483
Kzo9I GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
LguI GCTCTTC 1 cut(s) 2209
LmnI GCTCC 2 cut(s) 290, 688
Lsp1109I GCAGC 7 cut(s) 328, 653, 656, 1706, 1870, 1898, 2211
LweI GCATC 7 cut(s) 172, 224, 268, 656, 1366, 1397, 1882
MaeI CTAG 9 cut(s) 251, 590, 653, 809, 1076, 1407, 1520, 1679, 2169
MaeII ACGT 3 cut(s) 78, 559, 2153
MaeIII GTNAC 8 cut(s) 40, 113, 171, 528, 723, 914, 1181, 2071
MalI GATC 6 cut(s) 537, 804, 1516, 1545, 1660, 1815
MboI GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
MboII GAAGA 5 cut(s) 1469, 1602, 1635, 1823, 2196
MfeI CAATTG 2 cut(s) 167, 1139
MflI RGATCY 2 cut(s) 802, 1813
MhlI GDGCHC 6 cut(s) 270, 482, 598, 1210, 1510, 1571
MlyI GAGTC 3 cut(s) 1295, 1739, 1970
MmeI TCCRAC 1 cut(s) 578
Mph1103I ATGCAT 3 cut(s) 104, 1381, 1791
MroI TCCGGA 1 cut(s) 1483
MslI CAYNNNNRTG 4 cut(s) 36, 197, 1257, 1982
MspA1I CMGCKG 2 cut(s) 947, 1889
MspI CCGG 6 cut(s) 716, 930, 1178, 1402, 1484, 2036
MspR9I CCNGG 7 cut(s) 717, 1115, 1179, 1307, 1427, 1496, 2036
MunI CAATTG 2 cut(s) 167, 1139
Mva1269I GAATGC 2 cut(s) 880, 1789
MvaI CCWGG 4 cut(s) 1115, 1307, 1427, 1496
MvnI CGCG 2 cut(s) 20, 411
MwoI GCNNNNNNNGC 5 cut(s) 227, 278, 875, 1385, 1892
NciI CCSGG 3 cut(s) 717, 1179, 2036
NcoI CCATGG 1 cut(s) 1977
NdeI CATATG 1 cut(s) 837
NdeII GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
NlaIV GGNNCC 4 cut(s) 286, 447, 595, 2034
NmuCI GTSAC 3 cut(s) 40, 723, 914
NsiI ATGCAT 3 cut(s) 104, 1381, 1791
NspI RCATGY 1 cut(s) 1789
OliI CACNNNNGTG 1 cut(s) 197
PagI TCATGA 1 cut(s) 1983
PasI CCCWGGG 1 cut(s) 1306
PciSI GCTCTTC 1 cut(s) 2209
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 1255
PctI GAATGC 2 cut(s) 880, 1789
PfeI GAWTC 4 cut(s) 245, 581, 1338, 1943
PinAI ACCGGT 1 cut(s) 929
PkrI GCNGC 9 cut(s) 19, 343, 643, 646, 1696, 1804, 1885, 1888, 2201
PleI GAGTC 3 cut(s) 1295, 1738, 1969
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 3 cut(s) 1295, 1738, 1969
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 2 cut(s) 446, 1537
PshBI ATTAAT 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 1571
Psp5II RGGWCCY 2 cut(s) 446, 1537
Psp6I CCWGG 4 cut(s) 1113, 1305, 1425, 1494
PspCI CACGTG 1 cut(s) 560
PspEI GGTNACC 1 cut(s) 1181
PspGI CCWGG 4 cut(s) 1113, 1305, 1425, 1494
PspN4I GGNNCC 4 cut(s) 286, 447, 595, 2034
PspPI GGNCC 6 cut(s) 317, 446, 932, 1438, 1537, 2032
PspPPI RGGWCCY 2 cut(s) 446, 1537
PsrI GAACNNNNNNTAC 2 cut(s) 1851, 1883
PstI CTGCAG 2 cut(s) 223, 1888
PsuI RGATCY 2 cut(s) 802, 1813
PvuII CAGCTG 2 cut(s) 947, 1889
RsaI GTAC 3 cut(s) 708, 1460, 1753
RsaNI GTAC 3 cut(s) 707, 1459, 1752
RseI CAYNNNNRTG 4 cut(s) 36, 197, 1257, 1982
SacI GAGCTC 1 cut(s) 1571
SapI GCTCTTC 1 cut(s) 2209
SatI GCNGC 9 cut(s) 18, 342, 642, 645, 1695, 1803, 1884, 1887, 2200
Sau3AI GATC 6 cut(s) 535, 802, 1514, 1543, 1658, 1813
Sau96I GGNCC 6 cut(s) 317, 446, 932, 1438, 1537, 2032
SchI GAGTC 3 cut(s) 1295, 1739, 1970
ScrFI CCNGG 7 cut(s) 717, 1115, 1179, 1307, 1427, 1496, 2036
SduI GDGCHC 6 cut(s) 270, 482, 598, 1210, 1510, 1571
SfaNI GCATC 7 cut(s) 172, 224, 268, 656, 1366, 1397, 1882
SfcI CTRYAG 3 cut(s) 219, 942, 1884
SinI GGWCC 5 cut(s) 317, 446, 932, 1438, 1537
SmiMI CAYNNNNRTG 4 cut(s) 36, 197, 1257, 1982
SmlI CTYRAG 4 cut(s) 23, 1145, 1416, 1901
SmoI CTYRAG 4 cut(s) 23, 1145, 1416, 1901
SpeI ACTAGT 1 cut(s) 808
SsiI CCGC 8 cut(s) 18, 34, 161, 796, 1745, 1749, 1803, 2197
SspMI CTAG 9 cut(s) 251, 590, 653, 809, 1076, 1407, 1520, 1679, 2169
SstI GAGCTC 1 cut(s) 1571
StyD4I CCNGG 7 cut(s) 715, 1113, 1177, 1305, 1425, 1494, 2034
StyI CCWWGG 3 cut(s) 652, 1533, 1977
TaaI ACNGT 6 cut(s) 88, 311, 774, 884, 980, 1413
TaiI ACGT 3 cut(s) 81, 562, 2156
TaqI TCGA 3 cut(s) 1249, 1297, 1661
TatI WGTACW 1 cut(s) 706
TauI GCSGC 2 cut(s) 20, 1805
TfiI GAWTC 4 cut(s) 245, 581, 1338, 1943
TscAI CASTG 3 cut(s) 202, 1515, 1813
TseFI GTSAC 3 cut(s) 40, 723, 914
TseI GCWGC 7 cut(s) 341, 641, 644, 1694, 1883, 1886, 2199
Tsp45I GTSAC 3 cut(s) 40, 723, 914
TspDTI ATGAA 9 cut(s) 342, 347, 861, 1080, 1320, 1816, 1923, 2075, 2114
TspGWI ACGGA 5 cut(s) 193, 309, 1752, 2003, 2063
TspRI CASTG 3 cut(s) 202, 1515, 1813
VpaK11BI GGWCC 5 cut(s) 317, 446, 932, 1438, 1537
VspI ATTAAT 1 cut(s) 1106
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 7 cut(s) 181, 260, 1242, 1463, 1626, 1738, 2139
XbaI TCTAGA 1 cut(s) 2168
XceI RCATGY 1 cut(s) 1789
XcmI CCANNNNNNNNNTGG 1 cut(s) 999
XmaJI CCTAGG 1 cut(s) 652
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 9 cut(s) 251, 590, 653, 809, 1076, 1407, 1520, 1679, 2169
Zsp2I ATGCAT 3 cut(s) 104, 1381, 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.