MD08G1052200.v1.1
ERF Family

DnaJ Hsp40 cysteine-rich domain superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015264
Physical Location & Seq
Reverse (-)
4095649 .. 4097466
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1052200.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGCGAGCTCTGTGTCCTGCAGGCCGTCGATTGTGGCCAGACCGATTCCAAACGACGGTTTTCTATCGTCATCGGAAGCTCTAAATCGTGCCGGGGCTGTTGTACCTTACGGTACAAGCACGAGGGCCCTCCGAAGGGTTTGCGGCGTGCGAGCTTCAGCTGTGGATTCGTACGAAGGTTCATCTAATTTCGCTAATCGCATGGAAAAAGCATGGTTAATCTCTAAGCAACCAAGGCCTATTGCTTGTTCTTCTTGCAACTCAAATGGTCATATCGAATGCAAATGGTGTGCCGGAACAGGCTTCTTTATTCTCGGCGATAACATGCTCTGCCAGGTCTCTTCGAAAAGTACAAGTTGTGTTATTTGTACCGGTAAGGTGAGTAAAATCTTCACGGAGCCTGTTGGTGATTCTTGCTATCGTCTTCAATTTCCGATCTGCATTTGCATTTTCTGCTATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.6

Weight (kDa)

8.95

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24860
fragaria_vesca FvH4_2g41340 FvH4_2g41340
malus_domestica MD08G1052200.v1.1 MD15G1043300.v1.1
prunus_persica Prupe.1G396700_v2.0.a1
pyrus_communis pycom15g04020
rosa_chinensis RchiOBHm_Chr6g0299201
rosa_laevigata RLG00000011454
rosa_roxburghii Rroxscaffold_7G00168920
rosa_rugosa Rorug06G0286200
rosa_samantha Rh6AG397100 Rh6BG405800 Rh6CG411900 Rh6DG398600
rosa_wichuraiana Rw6G034780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 5 cut(s) 105, 115, 173, 352, 370
AfiI CCNNNNNNNGG 3 cut(s) 56, 135, 136
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 1 cut(s) 428
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 4 cut(s) 9, 80, 155, 161
AluI AGCT 4 cut(s) 9, 80, 155, 161
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 343
AoxI GGCC 4 cut(s) 23, 36, 126, 236
ApaI GGGCCC 1 cut(s) 130
AsiGI ACCGGT 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 126, 127
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 391, 419
AsuII TTCGAA 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 130
BalI TGGCCA 1 cut(s) 38
BanII GRGCYC 2 cut(s) 11, 130
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 10
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 343
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 145
BlsI GCNGC 1 cut(s) 146
Bme1390I CCNGG 2 cut(s) 94, 335
BmgT120I GGNCC 2 cut(s) 126, 127
BmiI GGNNCC 2 cut(s) 128, 399
BmrFI CCNGG 2 cut(s) 94, 335
BpiI GAAGAC 1 cut(s) 416
Bpu14I TTCGAA 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 93, 233
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 135, 136
Bse118I RCCGGY 1 cut(s) 371
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 93, 233
BseLI CCNNNNNNNGG 3 cut(s) 56, 135, 136
BseSI GKGCMC 1 cut(s) 130
BshFI GGCC 4 cut(s) 25, 38, 128, 238
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 3 cut(s) 93, 294, 372
BsiWI CGTACG 1 cut(s) 171
BslI CCNNNNNNNGG 3 cut(s) 56, 135, 136
BsmAI GTCTC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 4 cut(s) 25, 38, 128, 238
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 344
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 11, 130
Bsp143I GATC 1 cut(s) 435
BspACI CCGC 1 cut(s) 144
BspANI GGCC 4 cut(s) 25, 38, 128, 238
BspLI GGNNCC 2 cut(s) 128, 399
BspMAI CTGCAG 1 cut(s) 23
BspT104I TTCGAA 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 371
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 93, 233
BssMI GATC 1 cut(s) 435
BssSI CACGAG 1 cut(s) 121
BssT1I CCWWGG 1 cut(s) 233
Bst2BI CACGAG 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 59, 113
Bst6I CTCTTC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 453
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 7, 23, 149, 153
BstDEI CTNAG 1 cut(s) 225
BstKTI GATC 1 cut(s) 438
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 1 cut(s) 435
BstMWI GCNNNNNNNGC 2 cut(s) 235, 453
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 92, 333
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 416
BsuRI GGCC 4 cut(s) 25, 38, 128, 238
Cac8I GCNNGC 4 cut(s) 7, 23, 149, 153
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 2 cut(s) 126, 127
Csp6I GTAC 5 cut(s) 104, 114, 172, 351, 369
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 3 cut(s) 202, 213, 325
CviQI GTAC 5 cut(s) 104, 114, 172, 351, 369
DdeI CTNAG 1 cut(s) 225
DpnI GATC 1 cut(s) 437
DpnII GATC 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 233
Eco147I AGGCCT 1 cut(s) 238
Eco24I GRGCYC 2 cut(s) 11, 130
Eco31I GGTCTC 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 126, 127
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 233
EcoT38I GRGCYC 2 cut(s) 11, 130
ErhI CCWWGG 1 cut(s) 233
FaeI CATG 3 cut(s) 205, 216, 328
FaiI YATR 5 cut(s) 203, 214, 273, 326, 459
FatI CATG 3 cut(s) 201, 212, 324
Fnu4HI GCNGC 1 cut(s) 145
FriOI GRGCYC 2 cut(s) 11, 130
Fsp4HI GCNGC 1 cut(s) 145
GluI GCNGC 1 cut(s) 145
HaeIII GGCC 4 cut(s) 25, 38, 128, 238
HapII CCGG 3 cut(s) 93, 294, 372
Hin1II CATG 3 cut(s) 205, 216, 328
HinfI GANTC 3 cut(s) 46, 167, 410
HpaII CCGG 3 cut(s) 93, 294, 372
HphI GGTGA 2 cut(s) 391, 419
Hpy188I TCNGA 3 cut(s) 76, 134, 435
Hpy99I CGWCG 2 cut(s) 31, 59
HpyAV CCTTC 2 cut(s) 129, 170
HpyCH4III ACNGT 2 cut(s) 59, 113
HpyCH4V TGCA 5 cut(s) 21, 258, 282, 441, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 453
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 3 cut(s) 205, 216, 328
Kzo9I GATC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 397
MalI GATC 1 cut(s) 437
MboI GATC 1 cut(s) 435
MboII GAAGA 4 cut(s) 243, 333, 382, 416
MhlI GDGCHC 2 cut(s) 11, 130
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 2 cut(s) 187, 428
MluNI TGGCCA 1 cut(s) 38
MnlI CCTC 2 cut(s) 117, 140
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 3 cut(s) 93, 294, 372
MspR9I CCNGG 2 cut(s) 94, 335
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 2 cut(s) 235, 453
NciI CCSGG 1 cut(s) 94
NdeII GATC 1 cut(s) 435
NlaIII CATG 3 cut(s) 205, 216, 328
NlaIV GGNNCC 2 cut(s) 128, 399
NmeAIII GCCGAG 1 cut(s) 294
NspI RCATGY 1 cut(s) 328
NspV TTCGAA 1 cut(s) 344
PceI AGGCCT 1 cut(s) 238
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 46, 167, 410
Pfl23II CGTACG 1 cut(s) 171
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 128, 399
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 2 cut(s) 126, 127
PstI CTGCAG 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 5 cut(s) 105, 115, 173, 352, 370
RsaNI GTAC 5 cut(s) 104, 114, 172, 351, 369
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 218
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 1 cut(s) 435
Sau96I GGNCC 2 cut(s) 126, 127
SbfI CCTGCAGG 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 94, 335
SdaI CCTGCAGG 1 cut(s) 23
SduI GDGCHC 2 cut(s) 11, 130
SetI ASST 8 cut(s) 11, 82, 109, 157, 163, 181, 339, 381
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 344
Sse8387I CCTGCAGG 1 cut(s) 23
Sse9I AATT 2 cut(s) 187, 428
SseBI AGGCCT 1 cut(s) 238
SsiI CCGC 1 cut(s) 144
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 92, 333
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 2 cut(s) 59, 113
TaqI TCGA 3 cut(s) 29, 276, 344
TaqII GACCGA 1 cut(s) 58
TasI AATT 2 cut(s) 187, 428
TatI WGTACW 1 cut(s) 350
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 3 cut(s) 46, 167, 410
Tru1I TTAA 1 cut(s) 218
Tru9I TTAA 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 171
TspGWI ACGGA 1 cut(s) 410
XceI RCATGY 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.