MD08G1071500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015553
Physical Location & Seq
Forward (+)
5672224 .. 5674385
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1071500.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGCGGCAAACAGTACATGGTTTCTCTTTCTTGTAGCTTCTGTACTCATCGTTTCAGCTTCAGCTGGCACCCAAATCAAAGTTACTAATAACCCGGCGGATAAGCTAGTTGATATAATTAACAGCAACAGAACTGCACACAAAGCATCATCCCTCTACGACAACCAGGGTTTGGCGTGCATTGCCCTGCAGTACATAAAAGCGTACCAAGGTGACTGCGATGCTGTAGGAGGGTCCGACGCCAAGAAGCCTCCTGATTCTGCATTTGCCGAAACATTTGCTCCCAACTGTGGTGTTCAAACCTCAACCCTCGCTCCAATCACTGGTCGTTTAATTGGCTGCCAGTCCAAATATGTCCACGCTGACGAAGCATTTTCGAAAATCTTGATTGAAAACAGCAAGGCCTTGGACATTCTCTACGATAAGAACCACACTGAAGTGGGAGCTGCTGCGAGTGGCAGTGATGGTGGAGCTCCGTATTTCTGGTGCGTGTTGTTCAGCAGCGGAAAAACTAACAGCACCTTTGTAACGGAGGGAGGTGAGGCTAAAATAACAAGGCCCGGATGCTTTAGTGGTGCTAATGACGATTGCAGTGAATCTAATCACCCAGCCAGTGGTGCCGATGATTGGTCTAGAAGCAGTCGTCTGTGGCCATTTTTCACCACGGCTCTGATTGCAATGTCGTATGCCTTTGGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

24.61

Weight (kDa)

5.29

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015553)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30950
malus_domestica MD08G1071500.v1.1 MD13G1011100.v1.1 MD15G1049700.v1.1
prunus_persica Prupe.1G404000_v2.0.a1
pyrus_communis pycom08g05940 pycom15g04840
rosa_chinensis RchiOBHm_Chr6g0311891
rosa_laevigata RLG00000010371
rosa_multiflora Rmu_sc0002888.1_g000053
rosa_roxburghii Rroxscaffold_7G00157250
rosa_rugosa Rorug06G0391200
rosa_samantha Rh6BG518700 Rh6CG524400 Rh6DG510000
rosa_wichuraiana Rw6G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 617
AccB7I CCANNNNNTGG 3 cut(s) 172, 323, 614
AciI CCGC 3 cut(s) 5, 98, 504
AcoI YGGCCR 1 cut(s) 650
AcuI CTGAAG 2 cut(s) 45, 456
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 4 cut(s) 16, 45, 194, 206
AfiI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
AgsI TTSAA 2 cut(s) 299, 392
AjnI CCWGG 1 cut(s) 165
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 6 cut(s) 38, 59, 65, 106, 446, 473
AluI AGCT 6 cut(s) 38, 59, 65, 106, 446, 473
Alw21I GWGCWC 1 cut(s) 475
AoxI GGCC 3 cut(s) 402, 557, 650
ApeKI GCWGC 4 cut(s) 339, 446, 449, 501
AspS9I GGNCC 2 cut(s) 234, 558
AsuC2I CCSGG 2 cut(s) 95, 561
AsuHPI GGTGA 4 cut(s) 224, 551, 596, 652
AsuII TTCGAA 1 cut(s) 377
AvaII GGWCC 1 cut(s) 234
BalI TGGCCA 1 cut(s) 652
BanI GGYRCC 2 cut(s) 68, 617
BanII GRGCYC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 4 cut(s) 326, 433, 436, 513
BccI CCATC 1 cut(s) 458
BceAI ACGGC 1 cut(s) 681
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 2 cut(s) 95, 561
BfaI CTAG 2 cut(s) 107, 633
BfmI CTRYAG 2 cut(s) 188, 225
BisI GCNGC 5 cut(s) 6, 340, 447, 450, 502
BlsI GCNGC 5 cut(s) 7, 341, 448, 451, 503
Bme1390I CCNGG 3 cut(s) 95, 167, 561
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 234, 558
BmiI GGNNCC 3 cut(s) 70, 235, 619
BmrFI CCNGG 3 cut(s) 95, 167, 561
BmsI GCATC 3 cut(s) 155, 211, 554
Bpu14I TTCGAA 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 95, 561
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 4 cut(s) 166, 208, 405, 663
BsaXI ACNNNNNCTCC 4 cut(s) 265, 295, 298, 328
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
Bse1I ACTGG 3 cut(s) 328, 343, 612
Bse3DI GCAATG 2 cut(s) 180, 684
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 4 cut(s) 166, 208, 405, 663
BseGI GGATG 2 cut(s) 149, 569
BseLI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
BseMI GCAATG 2 cut(s) 180, 684
BseNI ACTGG 3 cut(s) 328, 343, 612
BseXI GCAGC 4 cut(s) 326, 433, 436, 513
BseYI CCCAGC 1 cut(s) 607
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 3 cut(s) 404, 559, 652
BshNI GGYRCC 2 cut(s) 68, 617
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 2 cut(s) 95, 561
BslI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
BsnI GGCC 3 cut(s) 404, 559, 652
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 475
BspACI CCGC 3 cut(s) 5, 98, 504
BspANI GGCC 3 cut(s) 404, 559, 652
BspLI GGNNCC 3 cut(s) 70, 235, 619
BspMAI CTGCAG 1 cut(s) 192
BspT104I TTCGAA 1 cut(s) 377
BspT107I GGYRCC 2 cut(s) 68, 617
BsrDI GCAATG 2 cut(s) 180, 684
BsrI ACTGG 3 cut(s) 328, 343, 612
BssECI CCNNGG 4 cut(s) 166, 208, 405, 663
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 208, 405
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 14, 290
BstACI GRCGYC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 67, 178
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 149, 569
BstMWI GCNNNNNNNGC 5 cut(s) 143, 182, 368, 617, 674
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 93, 165, 559
BstSFI CTRYAG 2 cut(s) 188, 225
BstV1I GCAGC 4 cut(s) 326, 433, 436, 513
BsuRI GGCC 3 cut(s) 404, 559, 652
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 149, 569
BtsI GCAGTG 2 cut(s) 466, 598
BtsIMutI CAGTG 5 cut(s) 321, 432, 466, 598, 619
Cac8I GCNNGC 2 cut(s) 67, 178
Cfr13I GGNCC 2 cut(s) 234, 558
CseI GACGC 1 cut(s) 248
Csp6I GTAC 4 cut(s) 15, 44, 193, 205
CviAII CATG 1 cut(s) 18
CviQI GTAC 4 cut(s) 15, 44, 193, 205
EaeI YGGCCR 1 cut(s) 650
EciI GGCGGA 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 473
Eco130I CCWWGG 2 cut(s) 208, 405
Eco147I AGGCCT 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 473
Eco57I CTGAAG 2 cut(s) 45, 456
EcoICRI GAGCTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 208, 405
EcoT38I GRGCYC 1 cut(s) 475
ErhI CCWWGG 2 cut(s) 208, 405
FaeI CATG 1 cut(s) 21
FaiI YATR 5 cut(s) 19, 116, 197, 354, 687
FatI CATG 1 cut(s) 17
Fnu4HI GCNGC 5 cut(s) 6, 340, 447, 450, 502
FokI GGATG 2 cut(s) 136, 576
FriOI GRGCYC 1 cut(s) 475
Fsp4HI GCNGC 5 cut(s) 6, 340, 447, 450, 502
FspBI CTAG 2 cut(s) 107, 633
GluI GCNGC 5 cut(s) 6, 340, 447, 450, 502
GsaI CCCAGC 1 cut(s) 611
HaeIII GGCC 3 cut(s) 404, 559, 652
HapII CCGG 2 cut(s) 95, 561
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 1 cut(s) 21
HinfI GANTC 2 cut(s) 257, 596
HpaII CCGG 2 cut(s) 95, 561
HphI GGTGA 4 cut(s) 224, 551, 596, 652
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 2 cut(s) 238, 672
Hpy188III TCNNGA 3 cut(s) 254, 385, 633
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 242
HpyCH4III ACNGT 2 cut(s) 14, 290
HpyCH4V TGCA 6 cut(s) 137, 180, 190, 263, 591, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 182, 368, 617, 674
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 1 cut(s) 21
LmnI GCTCC 5 cut(s) 286, 319, 443, 470, 478
Lsp1109I GCAGC 4 cut(s) 326, 433, 436, 513
LweI GCATC 3 cut(s) 155, 211, 554
MaeI CTAG 2 cut(s) 107, 633
MaeIII GTNAC 3 cut(s) 82, 212, 526
MhlI GDGCHC 1 cut(s) 475
MlsI TGGCCA 1 cut(s) 652
MluCI AATT 2 cut(s) 117, 333
MluNI TGGCCA 1 cut(s) 652
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 164, 224, 261, 313, 320, 526, 530, 535
Mox20I TGGCCA 1 cut(s) 652
MscI TGGCCA 1 cut(s) 652
MseI TTAA 2 cut(s) 120, 332
MslI CAYNNNNRTG 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 652
MspA1I CMGCKG 2 cut(s) 65, 504
MspI CCGG 2 cut(s) 95, 561
MspR9I CCNGG 3 cut(s) 95, 167, 561
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 5 cut(s) 143, 182, 368, 617, 674
NciI CCSGG 2 cut(s) 95, 561
NlaIII CATG 1 cut(s) 21
NlaIV GGNNCC 3 cut(s) 70, 235, 619
NmuCI GTSAC 1 cut(s) 212
NspV TTCGAA 1 cut(s) 377
OliI CACNNNNGTG 1 cut(s) 437
PceI AGGCCT 1 cut(s) 404
PfeI GAWTC 2 cut(s) 257, 596
PflMI CCANNNNNTGG 3 cut(s) 172, 323, 614
PkrI GCNGC 5 cut(s) 7, 341, 448, 451, 503
Psp124BI GAGCTC 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 607
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 70, 235, 619
PspPI GGNCC 2 cut(s) 234, 558
PstI CTGCAG 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 4 cut(s) 16, 45, 194, 206
RsaNI GTAC 4 cut(s) 15, 44, 193, 205
RseI CAYNNNNRTG 1 cut(s) 437
SacI GAGCTC 1 cut(s) 475
SaqAI TTAA 2 cut(s) 120, 332
SatI GCNGC 5 cut(s) 6, 340, 447, 450, 502
Sau96I GGNCC 2 cut(s) 234, 558
ScrFI CCNGG 3 cut(s) 95, 167, 561
SduI GDGCHC 1 cut(s) 475
SfaNI GCATC 3 cut(s) 155, 211, 554
SfcI CTRYAG 2 cut(s) 188, 225
SfuI TTCGAA 1 cut(s) 377
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 437
Sse9I AATT 2 cut(s) 117, 333
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 3 cut(s) 5, 98, 504
SspMI CTAG 2 cut(s) 107, 633
SstI GAGCTC 1 cut(s) 475
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 93, 165, 559
StyI CCWWGG 2 cut(s) 208, 405
TaaI ACNGT 2 cut(s) 14, 290
TaqI TCGA 1 cut(s) 377
TasI AATT 2 cut(s) 117, 333
TatI WGTACW 3 cut(s) 14, 43, 192
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 257, 596
Tru1I TTAA 2 cut(s) 120, 332
Tru9I TTAA 2 cut(s) 120, 332
TscAI CASTG 5 cut(s) 328, 439, 466, 598, 619
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 4 cut(s) 339, 446, 449, 501
Tsp45I GTSAC 1 cut(s) 212
TspGWI ACGGA 2 cut(s) 465, 545
TspRI CASTG 5 cut(s) 328, 439, 466, 598, 619
Van91I CCANNNNNTGG 3 cut(s) 172, 323, 614
VpaK11BI GGWCC 1 cut(s) 234
XbaI TCTAGA 1 cut(s) 632
XspI CTAG 2 cut(s) 107, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.