MD13G1011100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015553
Physical Location & Seq
Reverse (-)
671882 .. 672454
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1011100.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGTAAATGTAGCACTCATTTGGTGCAACAGAACTACACACAAAGCATCATCCCTCTACGACAACCAGGGTTTGGCGTGCATTGCCCTGCAGTACATAAAAGCGTACCAAGGTGACTGCGATGCTGTAGGAGGGCCCGACGCCAAGAAGCCTCCTGGTTCTGCATTTGCCGAAACATTTGCTCCCAACTGTGGTGTTCAAACCTCAACCCTCGCTCCAATCCCCTGTCGTTTAATTGGCTGCCAGTCTAGATATGTCCACGCTGACGAAGCATTTTCGAAAATCTTGATTGAAAACAGCAAGGCCTTGGACATTCTCTACGATAAGAACAACATTGAAGTGGGAACTGCTGTGAGTGGCAGTGATGGTGGAGCTCCATATTTCTGGTGCGTGTTGTTCAGCAGCGGAAAAACTAACATCACCTTTGTAACGAATGGAGGTGAGGCTAAAATAACAAGGCCCGGATGCTTTAGTGGTGCTAATGACGATTGCAGTGAATCTAATCACCCGGCCAGTGGTGCCGATGATTGGTCTAGAAGCAGTCGTCTGTGGCCATTTTCATCACGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.3

Weight (kDa)

5.88

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015553)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30950
malus_domestica MD08G1071500.v1.1 MD13G1011100.v1.1 MD15G1049700.v1.1
prunus_persica Prupe.1G404000_v2.0.a1
pyrus_communis pycom08g05940 pycom15g04840
rosa_chinensis RchiOBHm_Chr6g0311891
rosa_laevigata RLG00000010371
rosa_multiflora Rmu_sc0002888.1_g000053
rosa_roxburghii Rroxscaffold_7G00157250
rosa_rugosa Rorug06G0391200
rosa_samantha Rh6BG518700 Rh6CG524400 Rh6DG510000
rosa_wichuraiana Rw6G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 73
AciI CCGC 1 cut(s) 405
AcoI YGGCCR 2 cut(s) 510, 551
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 2 cut(s) 95, 107
AfiI CCNNNNNNNGG 4 cut(s) 73, 191, 515, 528
AgsI TTSAA 3 cut(s) 200, 293, 338
AjnI CCWGG 2 cut(s) 66, 154
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
Alw21I GWGCWC 1 cut(s) 376
AoxI GGCC 5 cut(s) 134, 303, 458, 510, 551
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 240, 402
AspS9I GGNCC 3 cut(s) 134, 135, 459
AsuC2I CCSGG 2 cut(s) 462, 509
AsuHPI GGTGA 4 cut(s) 125, 412, 452, 497
AsuII TTCGAA 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 553
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 138, 376
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 227, 414
BccI CCATC 1 cut(s) 359
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 2 cut(s) 68, 156
BcnI CCSGG 2 cut(s) 462, 509
BfaI CTAG 2 cut(s) 249, 534
BfmI CTRYAG 2 cut(s) 89, 126
BisI GCNGC 2 cut(s) 241, 403
BlsI GCNGC 2 cut(s) 242, 404
Bme1390I CCNGG 4 cut(s) 68, 156, 462, 509
BmgT120I GGNCC 3 cut(s) 134, 135, 459
BmiI GGNNCC 2 cut(s) 136, 520
BmrFI CCNGG 4 cut(s) 68, 156, 462, 509
BmsI GCATC 3 cut(s) 56, 112, 455
Bpu14I TTCGAA 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 462, 509
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 67, 109, 306
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 199, 229
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 191, 515, 528
Bse1I ACTGG 2 cut(s) 244, 513
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 68, 156
BseDI CCNNGG 3 cut(s) 67, 109, 306
BseGI GGATG 2 cut(s) 50, 470
BseLI CCNNNNNNNGG 4 cut(s) 73, 191, 515, 528
BseMI GCAATG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 244, 513
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 2 cut(s) 227, 414
BshFI GGCC 5 cut(s) 136, 305, 460, 512, 553
BshNI GGYRCC 1 cut(s) 518
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 2 cut(s) 462, 509
BslI CCNNNNNNNGG 4 cut(s) 73, 191, 515, 528
BsnI GGCC 5 cut(s) 136, 305, 460, 512, 553
Bsp119I TTCGAA 1 cut(s) 278
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 138, 376
BspACI CCGC 1 cut(s) 405
BspANI GGCC 5 cut(s) 136, 305, 460, 512, 553
BspLI GGNNCC 2 cut(s) 136, 520
BspMAI CTGCAG 1 cut(s) 93
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 2 cut(s) 244, 513
BssECI CCNNGG 3 cut(s) 67, 109, 306
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 109, 306
Bst2UI CCWGG 2 cut(s) 68, 156
Bst4CI ACNGT 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 141
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 79
BstF5I GGATG 2 cut(s) 50, 470
BstMWI GCNNNNNNNGC 3 cut(s) 83, 269, 518
BstNI CCWGG 2 cut(s) 68, 156
BstSCI CCNGG 4 cut(s) 66, 154, 460, 507
BstSFI CTRYAG 2 cut(s) 89, 126
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 227, 414
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuRI GGCC 5 cut(s) 136, 305, 460, 512, 553
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 2 cut(s) 50, 470
BtsI GCAGTG 2 cut(s) 367, 499
BtsIMutI CAGTG 3 cut(s) 367, 499, 520
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 134, 135, 459
CseI GACGC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 94, 106
CviQI GTAC 2 cut(s) 94, 106
EaeI YGGCCR 2 cut(s) 510, 551
Ecl136II GAGCTC 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 109, 306
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 138, 376
Eco53kI GAGCTC 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 66, 154
EcoT14I CCWWGG 2 cut(s) 109, 306
EcoT38I GRGCYC 2 cut(s) 138, 376
ErhI CCWWGG 2 cut(s) 109, 306
FaiI YATR 3 cut(s) 98, 255, 379
Fnu4HI GCNGC 2 cut(s) 241, 403
FokI GGATG 2 cut(s) 37, 477
FriOI GRGCYC 2 cut(s) 138, 376
Fsp4HI GCNGC 2 cut(s) 241, 403
FspBI CTAG 2 cut(s) 249, 534
GluI GCNGC 2 cut(s) 241, 403
HaeIII GGCC 5 cut(s) 136, 305, 460, 512, 553
HapII CCGG 2 cut(s) 462, 509
HgaI GACGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 141
HinfI GANTC 1 cut(s) 497
HpaII CCGG 2 cut(s) 462, 509
HphI GGTGA 4 cut(s) 125, 412, 452, 497
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 572
Hpy188III TCNNGA 3 cut(s) 249, 286, 534
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4V TGCA 5 cut(s) 27, 81, 91, 164, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 269, 518
Hsp92I GRCGYC 1 cut(s) 141
LmnI GCTCC 4 cut(s) 187, 220, 371, 379
Lsp1109I GCAGC 2 cut(s) 227, 414
LweI GCATC 3 cut(s) 56, 112, 455
MaeI CTAG 2 cut(s) 249, 534
MaeIII GTNAC 2 cut(s) 113, 427
MhlI GDGCHC 2 cut(s) 138, 376
MlsI TGGCCA 1 cut(s) 553
MluCI AATT 1 cut(s) 234
MluNI TGGCCA 1 cut(s) 553
MnlI CCTC 7 cut(s) 65, 125, 162, 214, 221, 431, 436
Mox20I TGGCCA 1 cut(s) 553
MscI TGGCCA 1 cut(s) 553
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 553
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 2 cut(s) 462, 509
MspR9I CCNGG 4 cut(s) 68, 156, 462, 509
MvaI CCWGG 2 cut(s) 68, 156
MwoI GCNNNNNNNGC 3 cut(s) 83, 269, 518
NciI CCSGG 2 cut(s) 462, 509
NlaIV GGNNCC 2 cut(s) 136, 520
NmuCI GTSAC 1 cut(s) 113
NspV TTCGAA 1 cut(s) 278
PceI AGGCCT 1 cut(s) 305
PfeI GAWTC 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 73
PkrI GCNGC 2 cut(s) 242, 404
Psp124BI GAGCTC 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 66, 154
PspGI CCWGG 2 cut(s) 66, 154
PspN4I GGNNCC 2 cut(s) 136, 520
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 3 cut(s) 134, 135, 459
PstI CTGCAG 1 cut(s) 93
RsaI GTAC 2 cut(s) 95, 107
RsaNI GTAC 2 cut(s) 94, 106
RseI CAYNNNNRTG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 376
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 2 cut(s) 241, 403
Sau96I GGNCC 3 cut(s) 134, 135, 459
ScrFI CCNGG 4 cut(s) 68, 156, 462, 509
SduI GDGCHC 2 cut(s) 138, 376
SetI ASST 5 cut(s) 115, 206, 376, 425, 442
SfaNI GCATC 3 cut(s) 56, 112, 455
SfcI CTRYAG 2 cut(s) 89, 126
SfuI TTCGAA 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 1 cut(s) 234
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 1 cut(s) 405
SspMI CTAG 2 cut(s) 249, 534
SstI GAGCTC 1 cut(s) 376
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 4 cut(s) 66, 154, 460, 507
StyI CCWWGG 2 cut(s) 109, 306
TaaI ACNGT 1 cut(s) 191
TaqI TCGA 1 cut(s) 278
TasI AATT 1 cut(s) 234
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 3 cut(s) 367, 499, 520
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 2 cut(s) 240, 402
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 549
TspRI CASTG 3 cut(s) 367, 499, 520
Van91I CCANNNNNTGG 1 cut(s) 73
XbaI TCTAGA 2 cut(s) 248, 533
XspI CTAG 2 cut(s) 249, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.