MD15G1049700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015553
Physical Location & Seq
Reverse (-)
3389543 .. 3391623
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1049700.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGCGGCAAGCAGCACATTCTTTCTCTTTCTTGTAGTTTCTGTTCTTGTCGTCTCTGCTTCAGCTGGTACCCAAATCAAAGTTACTAACAACCCGGCAGATAAACTAGTCGATATAATTAACAGCAACAGAACTGCACACAAAGCATCATCCCTCTACAGCAACCAGGGTTTGGCGTGCATTGCCCTGCAGTACATAAAGGCATATGAGGGTGACTGCGATGCTGTAGGAGGAACTGACGCCAAGAAGCCTGCTGATTCTGCATTTGCCGAAACATTTGCTCCCAACTGTGGCGTTCAACCCGCAACCCTCACTCCAATCACCGGTCGTTTAATTGGCTGCCAGTCCAAATATGTCCACGCTGATGAAGCATTTTCAACAATCTTGATTGAAAACAGCAAGGGCTTGGACATTCTATACAATAAGAATCACACTGAAGTGGGAGCTGCTGCGAGTGGCAGTGATGGCGGAGCTCCATATTTCTGGTGTGTGCTGTTTAGTGGTGGAAAAACTAACAGCAGCTTCGTAACGGAGGGAGGTGAGGCTAAAATAACAAGGCCCGGATGCTTTAGTGGTGCTAATGATGACTGCAGCGAATCAAATCACCCGGCCAGTGGTGCCGATGATTGGTCTAGAAGCAGTCATCTGTGGCCATTTTTTGCCACGGCTCTGATTGCAATGTGGTATGCCTTTGGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

24.49

Weight (kDa)

5.27

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015553)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30950
malus_domestica MD08G1071500.v1.1 MD13G1011100.v1.1 MD15G1049700.v1.1
prunus_persica Prupe.1G404000_v2.0.a1
pyrus_communis pycom08g05940 pycom15g04840
rosa_chinensis RchiOBHm_Chr6g0311891
rosa_laevigata RLG00000010371
rosa_multiflora Rmu_sc0002888.1_g000053
rosa_roxburghii Rroxscaffold_7G00157250
rosa_rugosa Rorug06G0391200
rosa_samantha Rh6BG518700 Rh6CG524400 Rh6DG510000
rosa_wichuraiana Rw6G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 68
AccB1I GGYRCC 2 cut(s) 68, 617
AccB7I CCANNNNNTGG 1 cut(s) 172
AciI CCGC 3 cut(s) 5, 303, 468
AcoI YGGCCR 2 cut(s) 609, 650
AcuI CTGAAG 2 cut(s) 45, 456
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 70, 194
AfiI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
AgeI ACCGGT 1 cut(s) 323
AgsI TTSAA 3 cut(s) 299, 378, 392
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 1 cut(s) 165
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 4 cut(s) 65, 446, 473, 522
AluI AGCT 4 cut(s) 65, 446, 473, 522
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 1 cut(s) 58
AoxI GGCC 3 cut(s) 557, 609, 650
ApeKI GCWGC 6 cut(s) 12, 339, 446, 449, 519, 591
AsiGI ACCGGT 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 558
AsuC2I CCSGG 3 cut(s) 95, 561, 608
AsuHPI GGTGA 4 cut(s) 224, 313, 551, 596
BalI TGGCCA 1 cut(s) 652
BanI GGYRCC 2 cut(s) 68, 617
BanII GRGCYC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 6 cut(s) 24, 326, 433, 436, 531, 603
BccI CCATC 1 cut(s) 458
BceAI ACGGC 1 cut(s) 681
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 3 cut(s) 95, 561, 608
BcoDI GTCTC 1 cut(s) 58
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 2 cut(s) 107, 633
BfmI CTRYAG 4 cut(s) 157, 188, 225, 589
BisI GCNGC 7 cut(s) 6, 13, 340, 447, 450, 520, 592
BlsI GCNGC 7 cut(s) 7, 14, 341, 448, 451, 521, 593
Bme1390I CCNGG 4 cut(s) 95, 167, 561, 608
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 2 cut(s) 70, 619
BmrFI CCNGG 4 cut(s) 95, 167, 561, 608
BmsI GCATC 3 cut(s) 155, 211, 554
BpuMI CCSGG 3 cut(s) 95, 561, 608
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 166, 663
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 4 cut(s) 265, 295, 298, 328
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 343, 612
Bse3DI GCAATG 2 cut(s) 180, 684
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 166, 663
BseGI GGATG 2 cut(s) 149, 569
BseLI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
BseMI GCAATG 2 cut(s) 180, 684
BseNI ACTGG 2 cut(s) 343, 612
BseXI GCAGC 6 cut(s) 24, 326, 433, 436, 531, 603
BsgI GTGCAG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 559, 611, 652
BshNI GGYRCC 2 cut(s) 68, 617
BshTI ACCGGT 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 4 cut(s) 95, 324, 561, 608
BslI CCNNNNNNNGG 5 cut(s) 172, 290, 323, 614, 627
BsmAI GTCTC 1 cut(s) 58
BsmBI CGTCTC 1 cut(s) 58
BsnI GGCC 3 cut(s) 559, 611, 652
Bsp1286I GDGCHC 1 cut(s) 475
BspACI CCGC 3 cut(s) 5, 303, 468
BspANI GGCC 3 cut(s) 559, 611, 652
BspLI GGNNCC 2 cut(s) 70, 619
BspMAI CTGCAG 2 cut(s) 192, 593
BspT107I GGYRCC 2 cut(s) 68, 617
BsrDI GCAATG 2 cut(s) 180, 684
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 2 cut(s) 343, 612
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 166, 663
BssNI GRCGYC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 240
BstC8I GCNNGC 3 cut(s) 10, 178, 252
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 149, 569
BstMAI GTCTC 1 cut(s) 58
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 7 cut(s) 143, 182, 260, 368, 465, 617, 674
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 4 cut(s) 93, 165, 559, 606
BstSFI CTRYAG 4 cut(s) 157, 188, 225, 589
BstV1I GCAGC 6 cut(s) 24, 326, 433, 436, 531, 603
BstXI CCANNNNNNTGG 1 cut(s) 483
BsuRI GGCC 3 cut(s) 559, 611, 652
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 149, 569
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 3 cut(s) 432, 466, 619
Cac8I GCNNGC 3 cut(s) 10, 178, 252
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 558
CseI GACGC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 69, 193
CspAI ACCGGT 1 cut(s) 323
CviQI GTAC 2 cut(s) 69, 193
EaeI YGGCCR 2 cut(s) 609, 650
EciI GGCGGA 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 473
Eco24I GRGCYC 1 cut(s) 475
Eco53kI GAGCTC 1 cut(s) 473
Eco57I CTGAAG 2 cut(s) 45, 456
EcoICRI GAGCTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 58
FaiI YATR 8 cut(s) 116, 197, 205, 207, 354, 418, 478, 687
FauI CCCGC 1 cut(s) 310
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 7 cut(s) 6, 13, 340, 447, 450, 520, 592
FokI GGATG 2 cut(s) 136, 576
FriOI GRGCYC 1 cut(s) 475
Fsp4HI GCNGC 7 cut(s) 6, 13, 340, 447, 450, 520, 592
FspBI CTAG 2 cut(s) 107, 633
GluI GCNGC 7 cut(s) 6, 13, 340, 447, 450, 520, 592
HaeIII GGCC 3 cut(s) 559, 611, 652
HapII CCGG 4 cut(s) 95, 324, 561, 608
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 240
HinfI GANTC 3 cut(s) 257, 427, 596
HpaII CCGG 4 cut(s) 95, 324, 561, 608
HphI GGTGA 4 cut(s) 224, 313, 551, 596
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 672
Hpy188III TCNNGA 2 cut(s) 385, 633
Hpy8I GTNNAC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 6 cut(s) 137, 180, 190, 263, 591, 677
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 182, 260, 368, 465, 617, 674
Hsp92I GRCGYC 1 cut(s) 240
KpnI GGTACC 1 cut(s) 72
LmnI GCTCC 4 cut(s) 286, 443, 470, 478
Lsp1109I GCAGC 6 cut(s) 24, 326, 433, 436, 531, 603
LweI GCATC 3 cut(s) 155, 211, 554
MaeI CTAG 2 cut(s) 107, 633
MaeIII GTNAC 3 cut(s) 82, 212, 526
MhlI GDGCHC 1 cut(s) 475
MlsI TGGCCA 1 cut(s) 652
MluCI AATT 2 cut(s) 117, 333
MluNI TGGCCA 1 cut(s) 652
MnlI CCTC 7 cut(s) 164, 202, 224, 320, 526, 530, 535
Mox20I TGGCCA 1 cut(s) 652
MscI TGGCCA 1 cut(s) 652
MseI TTAA 2 cut(s) 120, 332
MslI CAYNNNNRTG 2 cut(s) 363, 437
Msp20I TGGCCA 1 cut(s) 652
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 4 cut(s) 95, 324, 561, 608
MspR9I CCNGG 4 cut(s) 95, 167, 561, 608
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 7 cut(s) 143, 182, 260, 368, 465, 617, 674
NciI CCSGG 3 cut(s) 95, 561, 608
NdeI CATATG 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 70, 619
NmuCI GTSAC 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 437
PfeI GAWTC 3 cut(s) 257, 427, 596
PflMI CCANNNNNTGG 1 cut(s) 172
PinAI ACCGGT 1 cut(s) 323
PkrI GCNGC 7 cut(s) 7, 14, 341, 448, 451, 521, 593
Psp124BI GAGCTC 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 70, 619
PspPI GGNCC 1 cut(s) 558
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 2 cut(s) 192, 593
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 70, 194
RsaNI GTAC 2 cut(s) 69, 193
RseI CAYNNNNRTG 2 cut(s) 363, 437
SacI GAGCTC 1 cut(s) 475
SaqAI TTAA 2 cut(s) 120, 332
SatI GCNGC 7 cut(s) 6, 13, 340, 447, 450, 520, 592
Sau96I GGNCC 1 cut(s) 558
ScrFI CCNGG 4 cut(s) 95, 167, 561, 608
SduI GDGCHC 1 cut(s) 475
SetI ASST 5 cut(s) 67, 448, 475, 524, 541
SfaNI GCATC 3 cut(s) 155, 211, 554
SfcI CTRYAG 4 cut(s) 157, 188, 225, 589
SmiMI CAYNNNNRTG 2 cut(s) 363, 437
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 2 cut(s) 117, 333
SsiI CCGC 3 cut(s) 5, 303, 468
SspMI CTAG 2 cut(s) 107, 633
SstI GAGCTC 1 cut(s) 475
StyD4I CCNGG 4 cut(s) 93, 165, 559, 606
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 1 cut(s) 111
TasI AATT 2 cut(s) 117, 333
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 257, 427, 596
Tru1I TTAA 2 cut(s) 120, 332
Tru9I TTAA 2 cut(s) 120, 332
TscAI CASTG 3 cut(s) 439, 466, 619
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 6 cut(s) 12, 339, 446, 449, 519, 591
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 381
TspGWI ACGGA 1 cut(s) 545
TspRI CASTG 3 cut(s) 439, 466, 619
Van91I CCANNNNNTGG 1 cut(s) 172
XbaI TCTAGA 1 cut(s) 632
XspI CTAG 2 cut(s) 107, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.