MD08G1093800.v1.1
MYB Family

bHLH-MYC and R2R3-MYB transcription factors N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014672
Physical Location & Seq
Forward (+)
7791325 .. 7793550
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1093800.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGGAAAGTGGACTTCCCATGCTTAATTGTCTCTTGCAGCACACGCTGAGGAGCCTCTGTTCATGTTCAGATTCTTCTTCCAATTCCTCCAAGTGGGTTTATGCTGTCTTCTGGAGAATCTTGCCTCGGAACTATCCTCCTCCAAAGTGGGATTATGGAGGGAGTGCTCTTGATCGATCCAAAGGAAACAGAAGGAACTGGATTCTTGTTTGGGAAGATGGGTTCTGTGATTTCTATGAATGTGAGCAAGCAGGAAGTGGGTACATTAAGGGGAGGTTTGGAGCTGACATCTTCTTCAAAATGTCACATGAAGTATATAATTATGGAGAAGGATTGCTTGGGAAAGTTGCAGCAGATAATAGTCACAGATGGATTTTCAGAGATACCGCAAATGAAGGTGATCCTAACTTCATTTCCTCTTGGAATGCATCCACTGAACCGCAACCAAGGGCATGGGAGTTTCAATTCCAATCAGGCATACAGACAATTGCCATCATTTCTGTTAGAGAAGGCATAATTCAACTCGGTTCATTCGACAAGGTATTGGAGGATCTCAATTTGGTGATCAGTATACAGAGGAAATTCAGCTACCTTCAGAGCATACCAGGGGTATTTGCCATGCAAAGACCGTACCTACCAATCCAACATCCCTACATCTTCAAACCAAATCCTCAGATGATTGGAACTCAAGATCAAACAGCTTTATCAGTCTACGAAAAGCACCAACTGACCGGTGTGAAAAGATTGTTCAGTGAAGTTCCTGATGATTCCCAAATCAAGTCCATAAACTTAGGCTGGAACACACCACAAAATGGATTAGCAGGATCACCACCTTGGCCAATTGCTCCTCTTCTGCCCTCTATTTCTTGCAGCCTTGGAGCTCTCCTATCGAAGCTGCCTTCGGTTAATATTCCATCGTATAATGCTGCTGAAGCCCCTGACATGCTTATGAGCAACAACAGGAACAATTGTGCAGATCAGATGCAGAAGGTTAATGGTGGCATTATGAAAATAGAGTCATCCTGTCATTTAGATGTAGCTCATGAAGAAAAGCACAGTTCCATAAACCCTAATTTGGGTTTGGAAAATGGAGGAGGGGTAGACTTAGATTCGGACCAGTACGAAAACAAGAGACTTCGGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bHLH-MYC_N

Protein Analysis

382

Amino Acids

42.7

Weight (kDa)

5.93

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 12 - 79 1.3e-07 bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH-MYC_N PF14215 98 - 195 7.4e-19 bHLH-MYC and R2R3-MYB transcription factors N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014672)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33720 FvH4_2g33720
malus_domestica MD08G1093800.v1.1
prunus_persica Prupe.1G430900_v2.0.a1 Prupe.1G430900_v2.0.a1 Prupe.1G430900_v2.0.a1
pyrus_communis pycom15g07220
rosa_chinensis RchiOBHm_Chr6g0308831
rosa_laevigata RLG00000010614
rosa_multiflora Rmu_sc0004046.1_g000008
rosa_roxburghii Rroxscaffold_7G00159880
rosa_rugosa Rorug06G0368400.1
rosa_samantha Rh6AG479900 Rh6BG489400 Rh6CG494900 Rh6DG480200
rosa_wichuraiana Rw6G041840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 812
AccI GTMKAC 3 cut(s) 571, 711, 1101
AciI CCGC 2 cut(s) 387, 440
AclWI GGATC 4 cut(s) 171, 395, 558, 832
AcoI YGGCCR 1 cut(s) 836
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 578, 951
AfaI GTAC 3 cut(s) 263, 632, 1121
AfiI CCNNNNNNNGG 4 cut(s) 93, 812, 1075, 1076
AgeI ACCGGT 1 cut(s) 731
AgsI TTSAA 4 cut(s) 298, 464, 521, 661
AjnI CCWGG 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AloI GAACNNNNNNTCC 4 cut(s) 43, 75, 206, 238
AluBI AGCT 6 cut(s) 284, 588, 701, 881, 895, 1040
AluI AGCT 6 cut(s) 284, 588, 701, 881, 895, 1040
Alw21I GWGCWC 2 cut(s) 169, 883
Alw26I GTCTC 2 cut(s) 35, 1126
AlwI GGATC 4 cut(s) 171, 395, 558, 832
AoxI GGCC 1 cut(s) 836
ApeKI GCWGC 5 cut(s) 37, 350, 870, 895, 926
ApoI RAATTY 1 cut(s) 581
AsiGI ACCGGT 1 cut(s) 731
AspS9I GGNCC 1 cut(s) 1114
AsuHPI GGTGA 3 cut(s) 410, 574, 819
AvaII GGWCC 1 cut(s) 1114
BalI TGGCCA 1 cut(s) 838
BanII GRGCYC 1 cut(s) 883
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 2 cut(s) 169, 883
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 5 cut(s) 49, 362, 882, 882, 913
BccI CCATC 4 cut(s) 212, 363, 500, 922
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 1 cut(s) 564
BcoDI GTCTC 2 cut(s) 35, 1126
BisI GCNGC 5 cut(s) 38, 351, 871, 896, 927
BlsI GCNGC 5 cut(s) 39, 352, 872, 897, 928
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 1 cut(s) 1114
BmgT120I GGNCC 1 cut(s) 1114
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 1 cut(s) 606
BmsI GCATC 2 cut(s) 437, 972
BpiI GAAGAC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 672
Bsa29I ATCGAT 1 cut(s) 175
BsaJI CCNNGG 5 cut(s) 125, 446, 605, 833, 874
BsaWI WCCGGW 1 cut(s) 731
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 1083, 1113
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 812, 1075, 1076
Bse118I RCCGGY 1 cut(s) 731
Bse1I ACTGG 2 cut(s) 203, 1117
BseBI CCWGG 1 cut(s) 606
BseCI ATCGAT 1 cut(s) 175
BseDI CCNNGG 5 cut(s) 125, 446, 605, 833, 874
BseGI GGATG 3 cut(s) 428, 646, 1019
BseLI CCNNNNNNNGG 4 cut(s) 93, 812, 1075, 1076
BseMII CTCAG 2 cut(s) 38, 686
BseNI ACTGG 2 cut(s) 203, 1117
BseRI GAGGAG 4 cut(s) 64, 129, 837, 1107
BseXI GCAGC 5 cut(s) 49, 362, 882, 882, 913
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 1 cut(s) 838
BshTI ACCGGT 1 cut(s) 731
BshVI ATCGAT 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 169, 883
BsiSI CCGG 1 cut(s) 732
BslI CCNNNNNNNGG 4 cut(s) 93, 812, 1075, 1076
BsmAI GTCTC 2 cut(s) 35, 1126
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 838
Bsp1286I GDGCHC 2 cut(s) 169, 883
Bsp143I GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
BspACI CCGC 2 cut(s) 387, 440
BspANI GGCC 1 cut(s) 838
BspCNI CTCAG 2 cut(s) 39, 685
BspDI ATCGAT 1 cut(s) 175
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 1 cut(s) 53
BspPI GGATC 4 cut(s) 171, 395, 558, 832
BsrFI RCCGGY 1 cut(s) 731
BsrI ACTGG 2 cut(s) 203, 1117
BssAI RCCGGY 1 cut(s) 731
BssECI CCNNGG 5 cut(s) 125, 446, 605, 833, 874
BssMI GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
BssNAI GTATAC 1 cut(s) 572
BssT1I CCWWGG 3 cut(s) 446, 833, 874
Bst1107I GTATAC 1 cut(s) 572
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 630, 1058
Bst6I CTCTTC 1 cut(s) 855
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 4 cut(s) 47, 672, 790, 1105
BstF5I GGATG 3 cut(s) 428, 646, 1019
BstKTI GATC 8 cut(s) 175, 179, 403, 553, 567, 694, 827, 979
BstMAI GTCTC 2 cut(s) 35, 1126
BstMBI GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
BstMWI GCNNNNNNNGC 2 cut(s) 43, 932
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 1 cut(s) 604
BstV1I GCAGC 5 cut(s) 49, 362, 882, 882, 913
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 453
BstYI RGATCY 1 cut(s) 550
BstZ17I GTATAC 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 175
BsuRI GGCC 1 cut(s) 838
BsuTUI ATCGAT 1 cut(s) 175
BtsCI GGATG 3 cut(s) 428, 646, 1019
BtsIMutI CAGTG 2 cut(s) 432, 757
Cac8I GCNNGC 1 cut(s) 249
CciI TCATGA 1 cut(s) 1042
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 1 cut(s) 1114
ClaI ATCGAT 1 cut(s) 175
Csp6I GTAC 3 cut(s) 262, 631, 1120
CspAI ACCGGT 1 cut(s) 731
CviAII CATG 7 cut(s) 19, 63, 308, 453, 619, 943, 1043
CviQI GTAC 3 cut(s) 262, 631, 1120
DdeI CTNAG 4 cut(s) 47, 672, 790, 1105
DpnI GATC 8 cut(s) 174, 178, 402, 552, 566, 693, 826, 978
DpnII GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
EaeI YGGCCR 1 cut(s) 836
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
Ecl136II GAGCTC 1 cut(s) 881
Eco130I CCWWGG 3 cut(s) 446, 833, 874
Eco24I GRGCYC 1 cut(s) 883
Eco47I GGWCC 1 cut(s) 1114
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 2 cut(s) 578, 951
EcoICRI GAGCTC 1 cut(s) 881
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 3 cut(s) 446, 833, 874
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 883
ErhI CCWWGG 3 cut(s) 446, 833, 874
FaeI CATG 7 cut(s) 22, 66, 311, 456, 622, 946, 1046
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FatI CATG 7 cut(s) 18, 62, 307, 452, 618, 942, 1042
FbaI TGATCA 1 cut(s) 564
FblI GTMKAC 3 cut(s) 571, 711, 1101
Fnu4HI GCNGC 5 cut(s) 38, 351, 871, 896, 927
FokI GGATG 3 cut(s) 415, 633, 1006
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 5 cut(s) 38, 351, 871, 896, 927
GluI GCNGC 5 cut(s) 38, 351, 871, 896, 927
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 1 cut(s) 838
HapII CCGG 1 cut(s) 732
Hin1II CATG 7 cut(s) 22, 66, 311, 456, 622, 946, 1046
HinfI GANTC 6 cut(s) 71, 117, 202, 767, 1016, 1109
HpaII CCGG 1 cut(s) 732
HphI GGTGA 3 cut(s) 410, 574, 819
Hpy166II GTNNAC 4 cut(s) 11, 572, 712, 1102
Hpy188I TCNGA 8 cut(s) 70, 129, 380, 597, 675, 981, 1114, 1140
Hpy188III TCNNGA 5 cut(s) 112, 170, 689, 761, 1043
Hpy8I GTNNAC 4 cut(s) 11, 572, 712, 1102
HpyAV CCTTC 7 cut(s) 186, 323, 389, 503, 602, 909, 982
HpyCH4III ACNGT 2 cut(s) 630, 1058
HpyCH4IV ACGT 1 cut(s) 1142
HpyCH4V TGCA 7 cut(s) 37, 350, 428, 622, 870, 974, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 932
HpyF3I CTNAG 4 cut(s) 47, 672, 790, 1105
HpySE526I ACGT 1 cut(s) 1142
Hsp92II CATG 7 cut(s) 22, 66, 311, 456, 622, 946, 1046
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
LmnI GCTCC 4 cut(s) 51, 281, 850, 878
Lsp1109I GCAGC 5 cut(s) 49, 362, 882, 882, 913
LweI GCATC 2 cut(s) 437, 972
MaeII ACGT 1 cut(s) 1142
MaeIII GTNAC 2 cut(s) 303, 362
MalI GATC 8 cut(s) 174, 178, 402, 552, 566, 693, 826, 978
MboI GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
MboII GAAGA 9 cut(s) 66, 69, 100, 227, 283, 286, 649, 842, 1058
MfeI CAATTG 3 cut(s) 486, 840, 967
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 169, 883
MlsI TGGCCA 1 cut(s) 838
MluNI TGGCCA 1 cut(s) 838
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 1 cut(s) 667
Mox20I TGGCCA 1 cut(s) 838
Mph1103I ATGCAT 1 cut(s) 430
MscI TGGCCA 1 cut(s) 838
MseI TTAA 4 cut(s) 24, 267, 906, 993
MslI CAYNNNNRTG 2 cut(s) 947, 1032
Msp20I TGGCCA 1 cut(s) 838
MspI CCGG 1 cut(s) 732
MspR9I CCNGG 1 cut(s) 606
MunI CAATTG 3 cut(s) 486, 840, 967
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 2 cut(s) 43, 932
NdeII GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
NlaIII CATG 7 cut(s) 22, 66, 311, 456, 622, 946, 1046
NlaIV GGNNCC 1 cut(s) 53
NmuCI GTSAC 2 cut(s) 303, 362
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 946
PagI TCATGA 1 cut(s) 1042
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 5 cut(s) 71, 117, 202, 767, 1109
PflMI CCANNNNNTGG 1 cut(s) 812
PinAI ACCGGT 1 cut(s) 731
PkrI GCNGC 5 cut(s) 39, 352, 872, 897, 928
PleI GAGTC 1 cut(s) 1024
PpsI GAGTC 1 cut(s) 1024
Psp124BI GAGCTC 1 cut(s) 883
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 3 cut(s) 263, 632, 1121
RsaNI GTAC 3 cut(s) 262, 631, 1120
RseI CAYNNNNRTG 2 cut(s) 947, 1032
SacI GAGCTC 1 cut(s) 883
SaqAI TTAA 4 cut(s) 24, 267, 906, 993
SatI GCNGC 5 cut(s) 38, 351, 871, 896, 927
Sau3AI GATC 8 cut(s) 172, 176, 400, 550, 564, 691, 824, 976
Sau96I GGNCC 1 cut(s) 1114
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 1 cut(s) 606
SduI GDGCHC 2 cut(s) 169, 883
SfaNI GCATC 2 cut(s) 437, 972
SinI GGWCC 1 cut(s) 1114
SmiMI CAYNNNNRTG 2 cut(s) 947, 1032
SmlI CTYRAG 1 cut(s) 687
SmoI CTYRAG 1 cut(s) 687
SsiI CCGC 2 cut(s) 387, 440
SspI AATATT 1 cut(s) 910
SstI GAGCTC 1 cut(s) 883
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 3 cut(s) 446, 833, 874
TaaI ACNGT 2 cut(s) 630, 1058
TaiI ACGT 1 cut(s) 1145
TaqI TCGA 3 cut(s) 175, 534, 890
TfiI GAWTC 5 cut(s) 71, 117, 202, 767, 1109
Tru1I TTAA 4 cut(s) 24, 267, 906, 993
Tru9I TTAA 4 cut(s) 24, 267, 906, 993
TscAI CASTG 2 cut(s) 439, 757
TseFI GTSAC 2 cut(s) 303, 362
TseI GCWGC 5 cut(s) 37, 350, 870, 895, 926
Tsp45I GTSAC 2 cut(s) 303, 362
TspDTI ATGAA 8 cut(s) 51, 252, 324, 400, 408, 519, 1022, 1059
TspRI CASTG 2 cut(s) 439, 757
Van91I CCANNNNNTGG 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 1114
XapI RAATTY 1 cut(s) 581
XceI RCATGY 1 cut(s) 946
XmiI GTMKAC 3 cut(s) 571, 711, 1101
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.