MD08G1125000.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012910
Physical Location & Seq
Forward (+)
11702517 .. 11704400
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1125000.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGAACAATCAAATCAACGTCCACTGCACCAGCCACCCTCAAATTTCATCTCCCGGCCGCCCGAACAACAATTCAAACGAACCTAACACAGCTCCCTTACTTCTCCAATCCTCAGATGAACTCTGCAGCTTCAAAGGCAGCCTAAAATGGTGGGCACTTGACCACTCCTCGTGCTTCGGAAAATTCCTATCTTACTTCACCTTCATCTTCTTAACAGTCCTAGTCCCAATCCTCACCTCCCTCTCTCTCAAACTCCCATCTGGGGACCCCATCTCATTCAACAAGCTAGTCCAACTTCCCGAGTCCAGCTTAGCCGCCATTGGGTTCATGACTCTTTCTGGCTTCTTCCGAAAATATGGACTGAGGCAACTCCTCTTCCTCGACGGTCTTACAGATGACTCTGGCTTTGTTCGTCGTGGATACACTCGTGAGCTTGACAAGGCCTTCAAGTACCTAGCTTGCATACTACTGCCGTCCTTTTTCATTGAGCTTGCCCACAAGATCATATTCTTCTCCAATGTCCAAATCTCACTGCCTCATATAAGCTCAAGCGTCCCAGTCAACTCAATTATGTTTGTTTTGGTTTTGGCTTCTTGGGTTTATAGGACTGGGGTGTTCTTGCTGGTTTGTGTTATGTTCCGGTTGACTTGCGAGCTACAAATACTTCGATTCGAAGGGCTTTACAAGTTGTTTGAAGGGCATGAATACGAATCCAGTATCATATTCCAGGAACATGTAAGGATAAGAAAGCAATTGAAGGTCACGAGCCACAGGTACCGCTTCTTTATCATCGGATGCATGTTTACCATCACGGTGAGTCAATTCGGGGCCTTGTTGCTGGTTTTGGCATCCAAGACGGAGAAGACTTTCCTCAACTCTGGTGATCTTGTGGTTTGTTCTGCAGTCGAGCTTAGTGGCTTGTTTTGTTGCCTAATGGAGGCAGCAAGAATTACTCATAGAGCTCAAGGAGTTGTAGCAATTGCAACAAGATGGCACATGCTCTTGACTTGTCCGAATGCCGGATCAGACCAGCAGTGGAAAAGCCGGCAGATTAGCTCAGAGGCAAATGGACGCTGTAGAGAGAGTGACTGTGAACATTCATCAGACATTTCCACTTCAGTTCCTCCACAGCAGCCTTCCTCATTTCAAACCAGGCAAGCTCTGGTGGCGTATTTGAGTCACAACAACGGAGGGATCACGCTGTATGGGTTTGCGCTTGATCGGGGGTTGCTTCACACACTATTTGCATTTGAGTTCTCTCTGGTGCTGTGGGTGCTCAGCAAAGTGGTTGTTTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

434

Amino Acids

48.64

Weight (kDa)

8.75

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 39 - 417 3.3e-141 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21080
fragaria_vesca FvH4_2g36520
malus_domestica MD08G1125000.v1.1 MD15G1104300.v1.1 MD15G1104400.v1.1 MD15G1104600.v1.1
prunus_persica Prupe.1G456600_v2.0.a1
pyrus_communis pycom08g10370
rosa_chinensis RchiOBHm_Chr6g0305351
rosa_laevigata RLG00000010897
rosa_multiflora Rmu_ssc0000148.1_g000004
rosa_roxburghii Rroxscaffold_7G00162880
rosa_rugosa Rorug06G0339800
rosa_samantha Rh6AG451500 Rh6BG450000 Rh6CG465600 Rh6DG452300
rosa_wichuraiana Rw6G039340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 774
AccB1I GGYRCC 1 cut(s) 774
AciI CCGC 3 cut(s) 58, 315, 778
AclWI GGATC 2 cut(s) 1030, 1202
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 2 cut(s) 42, 182
AcuI CTGAAG 1 cut(s) 1101
AfaI GTAC 2 cut(s) 452, 776
AfiI CCNNNNNNNGG 4 cut(s) 262, 321, 937, 1019
AflIII ACRYGT 1 cut(s) 733
AgsI TTSAA 7 cut(s) 75, 133, 280, 448, 695, 757, 1148
AjnI CCWGG 2 cut(s) 726, 1151
Alw21I GWGCWC 2 cut(s) 964, 1278
AlwI GGATC 2 cut(s) 1030, 1202
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 55, 441, 828
ApeKI GCWGC 4 cut(s) 126, 138, 941, 1132
ApoI RAATTY 2 cut(s) 42, 182
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
Asp700I GAANNNNTTC 1 cut(s) 866
Asp718I GGTACC 1 cut(s) 774
AspLEI GCGC 1 cut(s) 1216
AspS9I GGNCC 2 cut(s) 265, 828
AsuC2I CCSGG 1 cut(s) 54
AsuHPI GGTGA 4 cut(s) 190, 226, 826, 893
AsuII TTCGAA 1 cut(s) 672
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 265
BaeGI GKGCMC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
BanI GGYRCC 1 cut(s) 774
BanII GRGCYC 1 cut(s) 964
BauI CACGAG 3 cut(s) 169, 426, 763
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 2 cut(s) 964, 1278
BbvI GCAGC 4 cut(s) 138, 150, 953, 1144
BccI CCATC 4 cut(s) 265, 278, 815, 984
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 2 cut(s) 728, 1153
BciVI GTATCC 1 cut(s) 413
BcnI CCSGG 1 cut(s) 54
BfaI CTAG 3 cut(s) 221, 287, 455
BfmI CTRYAG 3 cut(s) 124, 900, 1075
BfuI GTATCC 1 cut(s) 413
BisI GCNGC 6 cut(s) 58, 127, 139, 315, 942, 1133
BlpI GCTNAGC 2 cut(s) 310, 1277
BlsI GCNGC 6 cut(s) 59, 128, 140, 316, 943, 1134
Bme1390I CCNGG 3 cut(s) 54, 728, 1153
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 265, 828
BmiI GGNNCC 4 cut(s) 266, 267, 776, 829
BmrFI CCNGG 3 cut(s) 54, 728, 1153
BmrI ACTGGG 2 cut(s) 551, 618
BmsI GCATC 2 cut(s) 785, 857
BmuI ACTGGG 2 cut(s) 551, 618
BpiI GAAGAC 1 cut(s) 869
Bpu1102I GCTNAGC 2 cut(s) 310, 1277
Bpu14I TTCGAA 1 cut(s) 672
BpuEI CTTGAG 2 cut(s) 532, 948
BpuMI CCSGG 1 cut(s) 54
BsaWI WCCGGW 1 cut(s) 639
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 321, 937, 1019
Bse118I RCCGGY 1 cut(s) 1044
Bse1I ACTGG 3 cut(s) 557, 613, 714
BseBI CCWGG 2 cut(s) 728, 1153
BseGI GGATG 2 cut(s) 800, 848
BseLI CCNNNNNNNGG 4 cut(s) 262, 321, 937, 1019
BseMII CTCAG 4 cut(s) 126, 353, 1071, 1291
BseNI ACTGG 3 cut(s) 557, 613, 714
BseRI GAGGAG 2 cut(s) 157, 362
BseSI GKGCMC 1 cut(s) 157
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 4 cut(s) 138, 150, 953, 1144
BsgI GTGCAG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 3 cut(s) 57, 443, 830
BshNI GGYRCC 1 cut(s) 774
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 964, 1278
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 54, 640, 1020, 1045
BslFI GGGAC 3 cut(s) 209, 278, 539
BslI CCNNNNNNNGG 4 cut(s) 262, 321, 937, 1019
BsmFI GGGAC 3 cut(s) 209, 278, 539
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 3 cut(s) 57, 443, 830
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 3 cut(s) 157, 964, 1278
Bsp143I GATC 5 cut(s) 501, 883, 1022, 1194, 1219
Bsp1720I GCTNAGC 2 cut(s) 310, 1277
BspACI CCGC 3 cut(s) 58, 315, 778
BspANI GGCC 3 cut(s) 57, 443, 830
BspCNI CTCAG 4 cut(s) 125, 354, 1070, 1290
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 4 cut(s) 266, 267, 776, 829
BspMAI CTGCAG 2 cut(s) 128, 904
BspPI GGATC 2 cut(s) 1030, 1202
BspT104I TTCGAA 1 cut(s) 672
BspT107I GGYRCC 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 1044
BsrI ACTGG 3 cut(s) 557, 613, 714
BssAI RCCGGY 1 cut(s) 1044
BssMI GATC 5 cut(s) 501, 883, 1022, 1194, 1219
BssSI CACGAG 3 cut(s) 169, 426, 763
Bst2BI CACGAG 3 cut(s) 169, 426, 763
Bst2UI CCWGG 2 cut(s) 728, 1153
Bst4CI ACNGT 4 cut(s) 217, 386, 814, 1091
Bst6I CTCTTC 1 cut(s) 380
BstBI TTCGAA 1 cut(s) 672
BstC8I GCNNGC 5 cut(s) 460, 492, 653, 1046, 1158
BstDEI CTNAG 6 cut(s) 112, 310, 362, 911, 1057, 1277
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 2 cut(s) 800, 848
BstHHI GCGC 1 cut(s) 1216
BstKTI GATC 5 cut(s) 504, 886, 1025, 1197, 1222
BstMBI GATC 5 cut(s) 501, 883, 1022, 1194, 1219
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 135, 1166, 1273
BstNI CCWGG 2 cut(s) 728, 1153
BstNSI RCATGY 3 cut(s) 737, 802, 1000
BstSCI CCNGG 3 cut(s) 52, 726, 1151
BstSFI CTRYAG 3 cut(s) 124, 900, 1075
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 4 cut(s) 138, 150, 953, 1144
BstV2I GAAGAC 1 cut(s) 869
BstZI CGGCCG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 3 cut(s) 57, 443, 830
BtsCI GGATG 2 cut(s) 800, 848
BtsI GCAGTG 3 cut(s) 22, 530, 1040
BtsIMutI CAGTG 3 cut(s) 22, 530, 1040
Cac8I GCNNGC 5 cut(s) 460, 492, 653, 1046, 1158
CciI TCATGA 1 cut(s) 327
CfoI GCGC 1 cut(s) 1216
Cfr10I RCCGGY 1 cut(s) 1044
Cfr13I GGNCC 2 cut(s) 265, 828
CseI GACGC 2 cut(s) 541, 1080
Csp6I GTAC 2 cut(s) 451, 775
CviAII CATG 5 cut(s) 328, 701, 734, 799, 997
CviQI GTAC 2 cut(s) 451, 775
DdeI CTNAG 6 cut(s) 112, 310, 362, 911, 1057, 1277
DpnI GATC 5 cut(s) 503, 885, 1024, 1196, 1221
DpnII GATC 5 cut(s) 501, 883, 1022, 1194, 1219
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 962
EclXI CGGCCG 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 964
Eco47I GGWCC 1 cut(s) 265
Eco52I CGGCCG 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 962
Eco57I CTGAAG 1 cut(s) 1101
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 962
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoO109I RGGNCCY 2 cut(s) 265, 828
EcoRII CCWGG 2 cut(s) 726, 1151
EcoT22I ATGCAT 1 cut(s) 800
EcoT38I GRGCYC 1 cut(s) 964
FaeI CATG 5 cut(s) 331, 704, 737, 802, 1000
FaqI GGGAC 3 cut(s) 209, 278, 539
FatI CATG 5 cut(s) 327, 700, 733, 798, 996
Fnu4HI GCNGC 6 cut(s) 58, 127, 139, 315, 942, 1133
FokI GGATG 2 cut(s) 807, 835
FriOI GRGCYC 1 cut(s) 964
Fsp4HI GCNGC 6 cut(s) 58, 127, 139, 315, 942, 1133
FspBI CTAG 3 cut(s) 221, 287, 455
GlaI GCGC 1 cut(s) 1215
GluI GCNGC 6 cut(s) 58, 127, 139, 315, 942, 1133
HaeIII GGCC 3 cut(s) 57, 443, 830
HapII CCGG 4 cut(s) 54, 640, 1020, 1045
HgaI GACGC 2 cut(s) 541, 1080
HhaI GCGC 1 cut(s) 1216
Hin1II CATG 5 cut(s) 331, 704, 737, 802, 1000
Hin6I GCGC 1 cut(s) 1214
HinP1I GCGC 1 cut(s) 1214
HincII GTYRAC 2 cut(s) 562, 645
HindII GTYRAC 2 cut(s) 562, 645
HinfI GANTC 7 cut(s) 302, 331, 398, 669, 710, 817, 1177
HpaII CCGG 4 cut(s) 54, 640, 1020, 1045
HphI GGTGA 4 cut(s) 190, 226, 826, 893
Hpy166II GTNNAC 5 cut(s) 22, 562, 645, 804, 1094
Hpy188I TCNGA 8 cut(s) 115, 179, 350, 794, 1014, 1027, 1060, 1105
Hpy188III TCNNGA 5 cut(s) 299, 328, 428, 763, 1003
Hpy8I GTNNAC 5 cut(s) 22, 562, 645, 804, 1094
Hpy99I CGWCG 2 cut(s) 386, 417
HpyAV CCTTC 6 cut(s) 211, 454, 668, 689, 751, 1146
HpyCH4III ACNGT 4 cut(s) 217, 386, 814, 1091
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 7 cut(s) 27, 126, 462, 798, 902, 983, 1247
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 1166, 1273
HpyF3I CTNAG 6 cut(s) 112, 310, 362, 911, 1057, 1277
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 5 cut(s) 331, 704, 737, 802, 1000
HspAI GCGC 1 cut(s) 1214
KflI GGGWCCC 1 cut(s) 265
KpnI GGTACC 1 cut(s) 778
KroI GCCGGC 1 cut(s) 1044
KroNI GCCGGC 1 cut(s) 1046
Kzo9I GATC 5 cut(s) 501, 883, 1022, 1194, 1219
LmnI GCTCC 1 cut(s) 97
Lsp1109I GCAGC 4 cut(s) 138, 150, 953, 1144
LweI GCATC 2 cut(s) 785, 857
MaeI CTAG 3 cut(s) 221, 287, 455
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 3 cut(s) 760, 1085, 1178
MalI GATC 5 cut(s) 503, 885, 1024, 1196, 1221
MboI GATC 5 cut(s) 501, 883, 1022, 1194, 1219
MboII GAAGA 5 cut(s) 199, 337, 367, 502, 874
MfeI CAATTG 2 cut(s) 752, 978
MhlI GDGCHC 3 cut(s) 157, 964, 1278
MluCI AATT 8 cut(s) 42, 70, 182, 567, 752, 821, 948, 978
MlyI GAGTC 5 cut(s) 311, 325, 392, 826, 1186
MmeI TCCRAC 1 cut(s) 316
Mph1103I ATGCAT 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 1044
MroXI GAANNNNTTC 1 cut(s) 866
MseI TTAA 2 cut(s) 212, 1300
MslI CAYNNNNRTG 1 cut(s) 812
MspI CCGG 4 cut(s) 54, 640, 1020, 1045
MspR9I CCNGG 3 cut(s) 54, 728, 1153
MunI CAATTG 2 cut(s) 752, 978
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 2 cut(s) 728, 1153
MwoI GCNNNNNNNGC 3 cut(s) 135, 1166, 1273
NaeI GCCGGC 1 cut(s) 1046
NciI CCSGG 1 cut(s) 54
NdeII GATC 5 cut(s) 501, 883, 1022, 1194, 1219
NgoMIV GCCGGC 1 cut(s) 1044
NlaIII CATG 5 cut(s) 331, 704, 737, 802, 1000
NlaIV GGNNCC 4 cut(s) 266, 267, 776, 829
NmuCI GTSAC 3 cut(s) 760, 1085, 1178
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 3 cut(s) 737, 802, 1000
NspV TTCGAA 1 cut(s) 672
PagI TCATGA 1 cut(s) 327
PceI AGGCCT 1 cut(s) 443
PciI ACATGT 1 cut(s) 733
PctI GAATGC 1 cut(s) 1021
PdiI GCCGGC 1 cut(s) 1046
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 2 cut(s) 669, 710
PfoI TCCNGGA 1 cut(s) 726
PkrI GCNGC 6 cut(s) 59, 128, 140, 316, 943, 1134
PleI GAGTC 5 cut(s) 310, 325, 392, 825, 1185
PpsI GAGTC 5 cut(s) 310, 325, 392, 825, 1185
PpuMI RGGWCCY 1 cut(s) 265
PscI ACATGT 1 cut(s) 733
Psp124BI GAGCTC 1 cut(s) 964
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 726, 1151
PspGI CCWGG 2 cut(s) 726, 1151
PspN4I GGNNCC 4 cut(s) 266, 267, 776, 829
PspPI GGNCC 2 cut(s) 265, 828
PspPPI RGGWCCY 1 cut(s) 265
PstI CTGCAG 2 cut(s) 128, 904
RsaI GTAC 2 cut(s) 452, 776
RsaNI GTAC 2 cut(s) 451, 775
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 964
SaqAI TTAA 2 cut(s) 212, 1300
SatI GCNGC 6 cut(s) 58, 127, 139, 315, 942, 1133
Sau3AI GATC 5 cut(s) 501, 883, 1022, 1194, 1219
Sau96I GGNCC 2 cut(s) 265, 828
SchI GAGTC 5 cut(s) 311, 325, 392, 826, 1186
ScrFI CCNGG 3 cut(s) 54, 728, 1153
SduI GDGCHC 3 cut(s) 157, 964, 1278
SfaNI GCATC 2 cut(s) 785, 857
SfcI CTRYAG 3 cut(s) 124, 900, 1075
SfuI TTCGAA 1 cut(s) 672
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 547, 963
SmoI CTYRAG 2 cut(s) 547, 963
Sse9I AATT 8 cut(s) 42, 70, 182, 567, 752, 821, 948, 978
SseBI AGGCCT 1 cut(s) 443
SsiI CCGC 3 cut(s) 58, 315, 778
SspMI CTAG 3 cut(s) 221, 287, 455
SstI GAGCTC 1 cut(s) 964
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 52, 726, 1151
TaaI ACNGT 4 cut(s) 217, 386, 814, 1091
TaiI ACGT 1 cut(s) 21
TaqI TCGA 4 cut(s) 381, 667, 672, 906
TasI AATT 8 cut(s) 42, 70, 182, 567, 752, 821, 948, 978
TauI GCSGC 2 cut(s) 60, 317
TfiI GAWTC 2 cut(s) 669, 710
Tru1I TTAA 2 cut(s) 212, 1300
Tru9I TTAA 2 cut(s) 212, 1300
TscAI CASTG 3 cut(s) 29, 537, 1040
TseFI GTSAC 3 cut(s) 760, 1085, 1178
TseI GCWGC 4 cut(s) 126, 138, 941, 1132
Tsp45I GTSAC 3 cut(s) 760, 1085, 1178
TspDTI ATGAA 8 cut(s) 17, 36, 132, 193, 316, 472, 717, 1089
TspGWI ACGGA 2 cut(s) 872, 1203
TspRI CASTG 3 cut(s) 29, 537, 1040
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 2 cut(s) 42, 182
XceI RCATGY 3 cut(s) 737, 802, 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XmnI GAANNNNTTC 1 cut(s) 866
XspI CTAG 3 cut(s) 221, 287, 455
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.