MD15G1104300.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012910
Physical Location & Seq
Forward (+)
7333833 .. 7335802
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1104300.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGAACAACCAAATCAATGTCAACTTCACCAGCCAGAGCCACTCCGAAATTATATCTCGCCACCCCCTCAATGACGACTCAAACCAACCCGACATTCCCTTACTTCTCCAATCCTCATATGAGCTATGCAGCTTCAGAGTCAGCCTCAAATGGTGGGCACTCGACCACTCCTCGTGCCTTGGAAAATTCGTATCTTATTTCACCTTCATCTTCTTAACAATCCTAGTCCCTATCGTCACCTCCCTCTCTCTAAATACCTCATCTGACCCTATCTCGTTCAACAAGCTAGTCCAACTTCCTGAGTCCGGCTTGGCTGCCATTGGCTTCATAACTCTGTCCGGCTTCTTCAGGAAATATGGCCTAAGACAACTTCTCTTCCTTGACGGTCTTGGAGACGACTCTGGCTTTGTTCGTCGCGAATACACTCGCGAGCTCAATAAGGCCTTCAAGTCCCTTGCTTGCATACTACTGCCTTCATTCTTCATTGAGCTTGCCCACAAGATCATATTCTTCTGCACTGTCAAGATCTCACTGCCTCATATTAGTTCAAGCGTCCCACTCAATTCCATTATGTTTGTTTTCGTTCTGGCTTCTTGGGTTTATAGAACTGGCGTGTTCTTGCTGGTTTGTGTTTTGTTTCGCTTAACTTGTGAGCTCCAAATACTTAGGTTCCAAGAACTACACAAGTTGTTCGAGGGGTGTGAATTCGATTCCAGCATCATATTTAAGGAACATGTAAGGATAAGGAAGCAATTGTGGGTTACCAGCCATAGGTACCGGTTCTTTATCATCGGATGCTTGTTTACAATCACCGTGAGCCAATTCGGGGCCTTGTTGCTGGTTTTGGCATCCAAGACGGAGAAGACTTTCCTCAACTCTGGTGATCTTGTGGTTTGCTCTGCCGTTGAGCTCAGCGGGTTGTTCTTGTGTCTAATGGAGGCAGCAAGAATAACTCATAGAGCTCAAGGAATTGTGGCAATTGCAACGAGATGGCACATGTTCTTGACTTGTCCATCTGCTTTATCAGATCAGCAGTGCAAAAAATGGCTGCGCTCCGAGGCTAGCGGACATCATGGAGAGAGTGACTCCGAACATTTATCCGACGTTTTCTCTTCAGTTCCTCAAGAGGAGCCTTCCTCATTCCAAACCAGGCAAGCTCTAGTGGTGTATTTGCGACACAACAATGGAGGGATCACGCTGTATGGATTTATGCTTGATCGGGGGTCGCTTCGCACACTGTTTGCATTTCAGTTCTCTTTGGTGCTGTGGATACTCAGCAGAGTGGTTGTTTTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

433

Amino Acids

49.09

Weight (kDa)

8.57

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 41 - 416 2.2e-140 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21080
fragaria_vesca FvH4_2g36520
malus_domestica MD08G1125000.v1.1 MD15G1104300.v1.1 MD15G1104400.v1.1 MD15G1104600.v1.1
prunus_persica Prupe.1G456600_v2.0.a1
pyrus_communis pycom08g10370
rosa_chinensis RchiOBHm_Chr6g0305351
rosa_laevigata RLG00000010897
rosa_multiflora Rmu_ssc0000148.1_g000004
rosa_roxburghii Rroxscaffold_7G00162880
rosa_rugosa Rorug06G0339800
rosa_samantha Rh6AG451500 Rh6BG450000 Rh6CG465600 Rh6DG452300
rosa_wichuraiana Rw6G039340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 774
AccB1I GGYRCC 1 cut(s) 774
AccII CGCG 2 cut(s) 417, 429
AciI CCGC 2 cut(s) 915, 1065
AclWI GGATC 1 cut(s) 1199
AcsI RAATTY 2 cut(s) 185, 704
AcuI CTGAAG 3 cut(s) 118, 331, 1098
AfaI GTAC 1 cut(s) 776
AfiI CCNNNNNNNGG 3 cut(s) 305, 771, 826
AflIII ACRYGT 2 cut(s) 733, 996
AgeI ACCGGT 1 cut(s) 777
AgsI TTSAA 3 cut(s) 280, 448, 549
AjnI CCWGG 1 cut(s) 1148
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 9 cut(s) 124, 132, 286, 433, 490, 655, 910, 962, 1157
AluI AGCT 9 cut(s) 124, 132, 286, 433, 490, 655, 910, 962, 1157
Alw21I GWGCWC 4 cut(s) 435, 657, 912, 964
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 1 cut(s) 1199
AoxI GGCC 3 cut(s) 358, 441, 828
ApeKI GCWGC 4 cut(s) 129, 314, 941, 1048
ApoI RAATTY 2 cut(s) 185, 704
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AsiGI ACCGGT 1 cut(s) 777
Asp700I GAANNNNTTC 1 cut(s) 866
Asp718I GGTACC 1 cut(s) 774
AspLEI GCGC 1 cut(s) 1053
AspS9I GGNCC 1 cut(s) 828
AsuHPI GGTGA 5 cut(s) 19, 193, 229, 802, 893
AsuNHI GCTAGC 1 cut(s) 1061
BaeGI GKGCMC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 774
BanII GRGCYC 4 cut(s) 435, 657, 912, 964
BauI CACGAG 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 4 cut(s) 435, 657, 912, 964
BbvI GCAGC 4 cut(s) 141, 301, 953, 1035
BccI CCATC 2 cut(s) 984, 1021
BceAI ACGGC 1 cut(s) 887
BciT130I CCWGG 1 cut(s) 1150
BciVI GTATCC 1 cut(s) 1263
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 4 cut(s) 224, 287, 1062, 1160
BfuI GTATCC 1 cut(s) 1263
BglII AGATCT 1 cut(s) 525
BisI GCNGC 4 cut(s) 130, 315, 942, 1049
BlpI GCTNAGC 1 cut(s) 911
BlsI GCNGC 4 cut(s) 131, 316, 943, 1050
Bme1390I CCNGG 1 cut(s) 1150
BmgT120I GGNCC 1 cut(s) 828
BmiI GGNNCC 4 cut(s) 671, 776, 829, 1131
BmrFI CCNGG 1 cut(s) 1150
BmsI GCATC 3 cut(s) 726, 785, 857
BmtI GCTAGC 1 cut(s) 1065
BpiI GAAGAC 1 cut(s) 869
BplI GAGNNNNNCTC 6 cut(s) 129, 161, 1070, 1102, 1121, 1153
Bpu1102I GCTNAGC 1 cut(s) 911
BpuEI CTTGAG 2 cut(s) 948, 1107
BsaJI CCNNGG 2 cut(s) 178, 1056
BsaWI WCCGGW 1 cut(s) 777
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 771, 826
Bse118I RCCGGY 1 cut(s) 777
Bse1I ACTGG 1 cut(s) 613
BseBI CCWGG 1 cut(s) 1150
BseDI CCNNGG 2 cut(s) 178, 1056
BseGI GGATG 2 cut(s) 800, 848
BseLI CCNNNNNNNGG 3 cut(s) 305, 771, 826
BseMII CTCAG 3 cut(s) 291, 925, 1288
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 160, 1142
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 4 cut(s) 141, 301, 953, 1035
BsgI GTGCAG 1 cut(s) 499
Bsh1236I CGCG 2 cut(s) 417, 429
BshFI GGCC 3 cut(s) 360, 443, 830
BshNI GGYRCC 1 cut(s) 774
BshTI ACCGGT 1 cut(s) 777
BsiHKAI GWGCWC 4 cut(s) 435, 657, 912, 964
BsiSI CCGG 3 cut(s) 306, 339, 778
BslFI GGGAC 3 cut(s) 212, 436, 539
BslI CCNNNNNNNGG 3 cut(s) 305, 771, 826
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 3 cut(s) 212, 436, 539
BsnI GGCC 3 cut(s) 360, 443, 830
Bsp1286I GDGCHC 5 cut(s) 160, 435, 657, 912, 964
Bsp143I GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
Bsp1720I GCTNAGC 1 cut(s) 911
Bsp68I TCGCGA 2 cut(s) 417, 429
BspACI CCGC 2 cut(s) 915, 1065
BspANI GGCC 3 cut(s) 360, 443, 830
BspCNI CTCAG 3 cut(s) 292, 924, 1287
BspFNI CGCG 2 cut(s) 417, 429
BspLI GGNNCC 4 cut(s) 671, 776, 829, 1131
BspOI GCTAGC 1 cut(s) 1065
BspPI GGATC 1 cut(s) 1199
BspT107I GGYRCC 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 777
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 777
BssECI CCNNGG 2 cut(s) 178, 1056
BssMI GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
BssSI CACGAG 1 cut(s) 172
BssT1I CCWWGG 1 cut(s) 178
Bst2BI CACGAG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 1150
Bst4CI ACNGT 4 cut(s) 386, 520, 814, 1239
Bst6I CTCTTC 2 cut(s) 380, 1117
BstC8I GCNNGC 5 cut(s) 431, 460, 492, 1063, 1155
BstDEI CTNAG 5 cut(s) 300, 362, 665, 911, 1274
BstEII GGTNACC 1 cut(s) 760
BstF5I GGATG 2 cut(s) 800, 848
BstFNI CGCG 2 cut(s) 417, 429
BstHHI GCGC 1 cut(s) 1053
BstKTI GATC 6 cut(s) 504, 528, 886, 1030, 1194, 1219
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
BstNI CCWGG 1 cut(s) 1150
BstNSI RCATGY 2 cut(s) 737, 1000
BstPI GGTNACC 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 1148
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 2 cut(s) 417, 429
BstV1I GCAGC 4 cut(s) 141, 301, 953, 1035
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BsuI GTATCC 1 cut(s) 1263
BsuRI GGCC 3 cut(s) 360, 443, 830
BtsCI GGATG 2 cut(s) 800, 848
BtsI GCAGTG 2 cut(s) 530, 1040
BtsIMutI CAGTG 4 cut(s) 516, 530, 1040, 1235
BtuMI TCGCGA 2 cut(s) 417, 429
Cac8I GCNNGC 5 cut(s) 431, 460, 492, 1063, 1155
CfoI GCGC 1 cut(s) 1053
Cfr10I RCCGGY 1 cut(s) 777
Cfr13I GGNCC 1 cut(s) 828
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 775
CspAI ACCGGT 1 cut(s) 777
CviAII CATG 3 cut(s) 734, 997, 1073
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 5 cut(s) 300, 362, 665, 911, 1274
DpnI GATC 6 cut(s) 503, 527, 885, 1029, 1193, 1218
DpnII GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
Eam1104I CTCTTC 2 cut(s) 380, 1117
EarI CTCTTC 2 cut(s) 380, 1117
Ecl136II GAGCTC 4 cut(s) 433, 655, 910, 962
Eco130I CCWWGG 1 cut(s) 178
Eco147I AGGCCT 1 cut(s) 443
Eco24I GRGCYC 4 cut(s) 435, 657, 912, 964
Eco53kI GAGCTC 4 cut(s) 433, 655, 910, 962
Eco57I CTGAAG 3 cut(s) 118, 331, 1098
Eco91I GGTNACC 1 cut(s) 760
EcoICRI GAGCTC 4 cut(s) 433, 655, 910, 962
EcoO109I RGGNCCY 1 cut(s) 828
EcoO65I GGTNACC 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 1148
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 4 cut(s) 435, 657, 912, 964
ErhI CCWWGG 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 3 cut(s) 737, 1000, 1076
FaqI GGGAC 3 cut(s) 212, 436, 539
FatI CATG 3 cut(s) 733, 996, 1072
FauI CCCGC 1 cut(s) 908
FauNDI CATATG 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 130, 315, 942, 1049
FokI GGATG 2 cut(s) 807, 835
FriOI GRGCYC 4 cut(s) 435, 657, 912, 964
Fsp4HI GCNGC 4 cut(s) 130, 315, 942, 1049
FspBI CTAG 4 cut(s) 224, 287, 1062, 1160
GlaI GCGC 1 cut(s) 1052
GluI GCNGC 4 cut(s) 130, 315, 942, 1049
HaeIII GGCC 3 cut(s) 360, 443, 830
HapII CCGG 3 cut(s) 306, 339, 778
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 1053
Hin1II CATG 3 cut(s) 737, 1000, 1076
Hin6I GCGC 1 cut(s) 1051
HinP1I GCGC 1 cut(s) 1051
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 6 cut(s) 77, 138, 302, 398, 710, 1085
HpaII CCGG 3 cut(s) 306, 339, 778
HphI GGTGA 5 cut(s) 19, 193, 229, 802, 893
Hpy166II GTNNAC 2 cut(s) 22, 804
Hpy188I TCNGA 8 cut(s) 46, 137, 265, 794, 1027, 1057, 1090, 1102
Hpy188III TCNNGA 7 cut(s) 299, 349, 416, 428, 523, 1003, 1124
Hpy8I GTNNAC 2 cut(s) 22, 804
Hpy99I CGWCG 2 cut(s) 417, 1106
HpyAV CCTTC 4 cut(s) 214, 454, 483, 1143
HpyCH4III ACNGT 4 cut(s) 386, 520, 814, 1239
HpyCH4IV ACGT 1 cut(s) 1104
HpyCH4V TGCA 7 cut(s) 129, 462, 516, 983, 1038, 1244, 1294
HpyF3I CTNAG 5 cut(s) 300, 362, 665, 911, 1274
HpySE526I ACGT 1 cut(s) 1104
Hsp92II CATG 3 cut(s) 737, 1000, 1076
HspAI GCGC 1 cut(s) 1051
KpnI GGTACC 1 cut(s) 778
Kzo9I GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
LmnI GCTCC 3 cut(s) 660, 1058, 1129
Lsp1109I GCAGC 4 cut(s) 141, 301, 953, 1035
LweI GCATC 3 cut(s) 726, 785, 857
MaeI CTAG 4 cut(s) 224, 287, 1062, 1160
MaeII ACGT 1 cut(s) 1104
MaeIII GTNAC 3 cut(s) 235, 760, 1082
MalI GATC 6 cut(s) 503, 527, 885, 1029, 1193, 1218
MboI GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
MboII GAAGA 7 cut(s) 202, 337, 367, 472, 502, 874, 1104
MfeI CAATTG 2 cut(s) 752, 978
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 5 cut(s) 160, 435, 657, 912, 964
MluCI AATT 8 cut(s) 48, 185, 562, 704, 752, 821, 969, 978
MlyI GAGTC 5 cut(s) 71, 147, 311, 392, 1079
MmeI TCCRAC 2 cut(s) 316, 1125
MroXI GAANNNNTTC 1 cut(s) 866
MseI TTAA 4 cut(s) 215, 644, 726, 1297
MslI CAYNNNNRTG 1 cut(s) 1182
MspA1I CMGCKG 1 cut(s) 915
MspI CCGG 3 cut(s) 306, 339, 778
MspR9I CCNGG 1 cut(s) 1150
MunI CAATTG 2 cut(s) 752, 978
MvaI CCWGG 1 cut(s) 1150
MvnI CGCG 2 cut(s) 417, 429
NdeI CATATG 1 cut(s) 118
NdeII GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
NheI GCTAGC 1 cut(s) 1061
NlaIII CATG 3 cut(s) 737, 1000, 1076
NlaIV GGNNCC 4 cut(s) 671, 776, 829, 1131
NmuCI GTSAC 2 cut(s) 235, 1082
NruI TCGCGA 2 cut(s) 417, 429
NspI RCATGY 2 cut(s) 737, 1000
PceI AGGCCT 1 cut(s) 443
PciI ACATGT 2 cut(s) 733, 996
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 1 cut(s) 710
PinAI ACCGGT 1 cut(s) 777
PkrI GCNGC 4 cut(s) 131, 316, 943, 1050
PleI GAGTC 5 cut(s) 71, 146, 310, 392, 1079
PpsI GAGTC 5 cut(s) 71, 146, 310, 392, 1079
PscI ACATGT 2 cut(s) 733, 996
Psp124BI GAGCTC 4 cut(s) 435, 657, 912, 964
Psp6I CCWGG 1 cut(s) 1148
PspEI GGTNACC 1 cut(s) 760
PspGI CCWGG 1 cut(s) 1148
PspN4I GGNNCC 4 cut(s) 671, 776, 829, 1131
PspPI GGNCC 1 cut(s) 828
PsuI RGATCY 1 cut(s) 525
RruI TCGCGA 2 cut(s) 417, 429
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
RseI CAYNNNNRTG 1 cut(s) 1182
SacI GAGCTC 4 cut(s) 435, 657, 912, 964
SaqAI TTAA 4 cut(s) 215, 644, 726, 1297
SatI GCNGC 4 cut(s) 130, 315, 942, 1049
Sau3AI GATC 6 cut(s) 501, 525, 883, 1027, 1191, 1216
Sau96I GGNCC 1 cut(s) 828
SchI GAGTC 5 cut(s) 71, 147, 311, 392, 1079
ScrFI CCNGG 1 cut(s) 1150
SduI GDGCHC 5 cut(s) 160, 435, 657, 912, 964
SfaNI GCATC 3 cut(s) 726, 785, 857
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 2 cut(s) 963, 1122
SmoI CTYRAG 2 cut(s) 963, 1122
Sse9I AATT 8 cut(s) 48, 185, 562, 704, 752, 821, 969, 978
SseBI AGGCCT 1 cut(s) 443
SsiI CCGC 2 cut(s) 915, 1065
SspMI CTAG 4 cut(s) 224, 287, 1062, 1160
SstI GAGCTC 4 cut(s) 435, 657, 912, 964
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 1 cut(s) 1148
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 4 cut(s) 386, 520, 814, 1239
TaiI ACGT 1 cut(s) 1107
TaqI TCGA 3 cut(s) 162, 693, 708
TasI AATT 8 cut(s) 48, 185, 562, 704, 752, 821, 969, 978
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 4 cut(s) 215, 644, 726, 1297
Tru9I TTAA 4 cut(s) 215, 644, 726, 1297
TscAI CASTG 4 cut(s) 523, 537, 1040, 1242
TseFI GTSAC 2 cut(s) 235, 1082
TseI GCWGC 4 cut(s) 129, 314, 941, 1048
Tsp45I GTSAC 2 cut(s) 235, 1082
TspDTI ATGAA 5 cut(s) 17, 196, 316, 465, 472
TspGWI ACGGA 1 cut(s) 872
TspRI CASTG 4 cut(s) 523, 537, 1040, 1242
XapI RAATTY 2 cut(s) 185, 704
XceI RCATGY 2 cut(s) 737, 1000
XmnI GAANNNNTTC 1 cut(s) 866
XspI CTAG 4 cut(s) 224, 287, 1062, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.