MD15G1104400.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012910
Physical Location & Seq
Forward (+)
7338302 .. 7339063
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1104400.v1.1.491

Sequence Viewer

Length: 762 bp
ATGAACAACCAAATCAATGTCCACTGCACCAGCCACCCACAAATTTCATCTCCCTGCGGCCAGAATGACGATGCCAACCAACCTAACATAGCTCCCTTACTTCTCCAATCCTCCGATGAACTCTGCAGCTTCAGAGTCAGCCTAAAATGGTGGGCACTCGACCACTCCTCATGCTTCGGAAAATTCCTATCTTACTTCACCTTCATCTTCTTAACAGTCCTAGTCCCAATCCTCACCTCCCTCTTTCTCAAACTCCCATCTGGGGACCCCATCTCATTCAACAAGCTAGTCCAACTTCCGGAGTCCGGCTTAGCTGCCATTGGCTTCATTACTCTTTCTTGCTTCTTCCGAAAATATGGCCTGAGGCAACTCCTCTTCCTTGACAGTCTTAGAGATGACTCTGGCTTTGTACATCACGGATACACTCGTGAGCTCAATAAGGTCTTCAAGTACCTAGCTTGCATACTACTGCCTTCATTTTTCATTGAGCTTACCCAGAAGATCATATTCTTCTCAACTATCCAACTCTCACTGCCTCATATAAGCTCAAGCGTCCCAGTCAATTCAATTATGTTTGTTTTGGTTTTAGCTTCTTGGGTTTATAGGACTGGGGTGTTCTTGCTGGTCTGTGTTATGTTCCGGTTGACTTGCGAGCTCCAAATTCTTCGATTCAAAGGGTTGAACAAGTTGTTAGAGGGGCATGAATCTGAATCCTGTTGTGCTAGGATAGCACCAAACCTTTATGGAACCAACTCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

254

Amino Acids

28.53

Weight (kDa)

8.33

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 39 - 234 6.1e-66 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21080
fragaria_vesca FvH4_2g36520
malus_domestica MD08G1125000.v1.1 MD15G1104300.v1.1 MD15G1104400.v1.1 MD15G1104600.v1.1
prunus_persica Prupe.1G456600_v2.0.a1
pyrus_communis pycom08g10370
rosa_chinensis RchiOBHm_Chr6g0305351
rosa_laevigata RLG00000010897
rosa_multiflora Rmu_ssc0000148.1_g000004
rosa_roxburghii Rroxscaffold_7G00162880
rosa_rugosa Rorug06G0339800
rosa_samantha Rh6AG451500 Rh6BG450000 Rh6CG465600 Rh6DG452300
rosa_wichuraiana Rw6G039340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 3 cut(s) 42, 182, 660
AcuI CTGAAG 1 cut(s) 115
AfaI GTAC 2 cut(s) 411, 452
AfiI CCNNNNNNNGG 3 cut(s) 262, 298, 305
AgsI TTSAA 5 cut(s) 280, 448, 567, 673, 682
Alw21I GWGCWC 2 cut(s) 435, 657
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 2 cut(s) 58, 358
ApeKI GCWGC 2 cut(s) 126, 314
ApoI RAATTY 3 cut(s) 42, 182, 660
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 2 cut(s) 190, 226
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 412, 445
BanII GRGCYC 2 cut(s) 435, 657
BauI CACGAG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 436
Bbv12I GWGCWC 2 cut(s) 435, 657
BbvI GCAGC 2 cut(s) 138, 301
BccI CCATC 2 cut(s) 265, 278
BciVI GTATCC 1 cut(s) 413
BfaI CTAG 4 cut(s) 221, 287, 455, 723
BfmI CTRYAG 1 cut(s) 124
BfuI GTATCC 1 cut(s) 413
BisI GCNGC 3 cut(s) 58, 127, 315
BlpI GCTNAGC 1 cut(s) 310
BlsI GCNGC 3 cut(s) 59, 128, 316
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 3 cut(s) 266, 267, 748
BmrI ACTGGG 2 cut(s) 551, 618
BmsI GCATC 1 cut(s) 61
BmuI ACTGGG 2 cut(s) 551, 618
BpiI GAAGAC 1 cut(s) 436
Bpu1102I GCTNAGC 1 cut(s) 310
BpuEI CTTGAG 2 cut(s) 532, 739
BsaWI WCCGGW 2 cut(s) 298, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 298, 305
Bse1I ACTGG 2 cut(s) 557, 613
Bse21I CCTNAGG 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 298
BseLI CCNNNNNNNGG 3 cut(s) 262, 298, 305
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 2 cut(s) 557, 613
BseRI GAGGAG 2 cut(s) 157, 362
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 2 cut(s) 138, 301
BsgI GTGCAG 1 cut(s) 10
BshFI GGCC 2 cut(s) 60, 360
BsiHKAI GWGCWC 2 cut(s) 435, 657
BsiSI CCGG 3 cut(s) 299, 306, 640
BslFI GGGAC 3 cut(s) 209, 278, 539
BslI CCNNNNNNNGG 3 cut(s) 262, 298, 305
BsmFI GGGAC 3 cut(s) 209, 278, 539
BsnI GGCC 2 cut(s) 60, 360
Bsp1286I GDGCHC 3 cut(s) 157, 435, 657
Bsp13I TCCGGA 1 cut(s) 298
Bsp1407I TGTACA 1 cut(s) 409
Bsp143I GATC 1 cut(s) 501
Bsp1720I GCTNAGC 1 cut(s) 310
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 60, 360
BspCNI CTCAG 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 266, 267, 748
BspMAI CTGCAG 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 409
BsrI ACTGG 2 cut(s) 557, 613
BssMI GATC 1 cut(s) 501
BssSI CACGAG 1 cut(s) 426
Bst2BI CACGAG 1 cut(s) 426
Bst4CI ACNGT 2 cut(s) 217, 386
Bst6I CTCTTC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 460, 653
BstDEI CTNAG 3 cut(s) 310, 362, 389
BstKTI GATC 1 cut(s) 504
BstMBI GATC 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstSFI CTRYAG 1 cut(s) 124
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 138, 301
BstV2I GAAGAC 1 cut(s) 436
Bsu36I CCTNAGG 1 cut(s) 362
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 2 cut(s) 60, 360
BtsI GCAGTG 2 cut(s) 22, 530
BtsIMutI CAGTG 2 cut(s) 22, 530
Cac8I GCNNGC 2 cut(s) 460, 653
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 410, 451
CviAII CATG 2 cut(s) 171, 701
CviQI GTAC 2 cut(s) 410, 451
DdeI CTNAG 3 cut(s) 310, 362, 389
DpnI GATC 1 cut(s) 503
DpnII GATC 1 cut(s) 501
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Ecl136II GAGCTC 2 cut(s) 433, 655
Eco24I GRGCYC 2 cut(s) 435, 657
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 2 cut(s) 433, 655
Eco57I CTGAAG 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 362
EcoICRI GAGCTC 2 cut(s) 433, 655
EcoO109I RGGNCCY 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 435, 657
FaeI CATG 2 cut(s) 174, 704
FaqI GGGAC 3 cut(s) 209, 278, 539
FatI CATG 2 cut(s) 170, 700
Fnu4HI GCNGC 3 cut(s) 58, 127, 315
FriOI GRGCYC 2 cut(s) 435, 657
Fsp4HI GCNGC 3 cut(s) 58, 127, 315
FspBI CTAG 4 cut(s) 221, 287, 455, 723
GluI GCNGC 3 cut(s) 58, 127, 315
HaeIII GGCC 2 cut(s) 60, 360
HapII CCGG 3 cut(s) 299, 306, 640
HgaI GACGC 1 cut(s) 541
Hin1II CATG 2 cut(s) 174, 704
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HinfI GANTC 6 cut(s) 135, 302, 398, 669, 704, 710
HpaII CCGG 3 cut(s) 299, 306, 640
HphI GGTGA 2 cut(s) 190, 226
Hpy166II GTNNAC 2 cut(s) 22, 645
Hpy188I TCNGA 5 cut(s) 115, 134, 179, 350, 709
Hpy188III TCNNGA 2 cut(s) 299, 428
Hpy8I GTNNAC 2 cut(s) 22, 645
HpyAV CCTTC 2 cut(s) 211, 483
HpyCH4III ACNGT 2 cut(s) 217, 386
HpyCH4V TGCA 3 cut(s) 27, 126, 462
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 3 cut(s) 310, 362, 389
Hsp92II CATG 2 cut(s) 174, 704
KflI GGGWCCC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 1 cut(s) 501
LmnI GCTCC 2 cut(s) 97, 660
Lsp1109I GCAGC 2 cut(s) 138, 301
LweI GCATC 1 cut(s) 61
MaeI CTAG 4 cut(s) 221, 287, 455, 723
MalI GATC 1 cut(s) 503
MboI GATC 1 cut(s) 501
MboII GAAGA 7 cut(s) 199, 337, 367, 436, 502, 511, 656
MhlI GDGCHC 3 cut(s) 157, 435, 657
MluCI AATT 5 cut(s) 42, 182, 562, 567, 660
MlyI GAGTC 3 cut(s) 144, 311, 392
MmeI TCCRAC 2 cut(s) 316, 547
MnlI CCTC 9 cut(s) 121, 178, 242, 247, 251, 357, 383, 546, 688
MroI TCCGGA 1 cut(s) 298
MseI TTAA 1 cut(s) 212
MspI CCGG 3 cut(s) 299, 306, 640
MwoI GCNNNNNNNGC 1 cut(s) 728
NdeII GATC 1 cut(s) 501
NlaIII CATG 2 cut(s) 174, 704
NlaIV GGNNCC 3 cut(s) 266, 267, 748
PfeI GAWTC 3 cut(s) 669, 704, 710
PkrI GCNGC 3 cut(s) 59, 128, 316
PleI GAGTC 3 cut(s) 143, 310, 392
PpsI GAGTC 3 cut(s) 143, 310, 392
PpuMI RGGWCCY 1 cut(s) 265
Psp124BI GAGCTC 2 cut(s) 435, 657
Psp5II RGGWCCY 1 cut(s) 265
PspN4I GGNNCC 3 cut(s) 266, 267, 748
PspPI GGNCC 1 cut(s) 265
PspPPI RGGWCCY 1 cut(s) 265
PstI CTGCAG 1 cut(s) 128
RsaI GTAC 2 cut(s) 411, 452
RsaNI GTAC 2 cut(s) 410, 451
SacI GAGCTC 2 cut(s) 435, 657
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 3 cut(s) 58, 127, 315
Sau3AI GATC 1 cut(s) 501
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 3 cut(s) 144, 311, 392
SduI GDGCHC 3 cut(s) 157, 435, 657
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 547, 754
SmoI CTYRAG 2 cut(s) 547, 754
Sse9I AATT 5 cut(s) 42, 182, 562, 567, 660
SsiI CCGC 1 cut(s) 57
SspMI CTAG 4 cut(s) 221, 287, 455, 723
SstI GAGCTC 2 cut(s) 435, 657
TaaI ACNGT 2 cut(s) 217, 386
TaqI TCGA 2 cut(s) 159, 667
TasI AATT 5 cut(s) 42, 182, 562, 567, 660
TatI WGTACW 1 cut(s) 409
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 3 cut(s) 669, 704, 710
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 2 cut(s) 29, 537
TseI GCWGC 2 cut(s) 126, 314
TspDTI ATGAA 8 cut(s) 17, 36, 132, 193, 316, 465, 472, 717
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 2 cut(s) 29, 537
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 3 cut(s) 42, 182, 660
XspI CTAG 4 cut(s) 221, 287, 455, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.