Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010285

Physical Location & Seq

Genomic Coordinates Reverse (-)

12673025 .. 12680445

Gene Structure 7421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1133600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3255 bp

ATGAGCCGACCAAGTACGCGCAGTAAAAATAAACGAAACAGACAAGGAGATAATGTTGACAACACTTCCGAAATATTGAGGAAAATACATGCAACAGGTGAAATTACAAATGAGGATATATATACGCTATACCAAATTTCAAAGCCAGTTTGTCAAGGCTGCCGTGTGAATTCTAAAGATAACCCAAACTGCTTTTGTGGGCTAATTCCACCTCCGAATGGAAGTAGGAAATCAGGCCTATGGCAGAAGACATCAGAGATTCTGCAAACTCTGGGCCCAGATCCTTCTCAGGACCTTCGTCCTTCTGCCGATTCTCCTGCAGGCCTCACAAATCTGGGTGCAACATGCTATGCTAATAGCATACTCCAATGTTTGTACATGAACAAGTCCTTCCGTGAAGGTATCTTATTGGTTGAACCTGAGGTTTTGGAACAGCAGCCAGTGTTGAATCAGCTTTCTCGGCTTTTTGCTCAGTTGCATGCCAGTAAATTGGCTTTTATAGACTCATCTCCATTTGTAAAAACACTGGAGTTAGATAATGGAGTTCAACAGGACAGTCATGAGTTTCTGACCTTACTTCTTTCTTTGCTTGAACGTTGTCTGAGCAACTCTAAAGTTTCAAGGGCAAAATCTATTGTTCAAGATCTTTTCCGTGGAAGTGTATCACATGTTACTAGGTGCTCACAATGTGGAAAAGACTCTGAAGCTTCTTCAAACATGGAAGACTTCTATGAGTTGGAGTTGAATGTGAAGGGTTTGAAAAGTTTGGGTGAGAGTTTAGATGATTACCTTAGTGTTGAAGAGCTCCATGGAGAGAATCAATATTTTTGTGAGTCTTGTAAATCAAGAGTCGATGCTACTCGAAGCATCAAGTTGCGCACATTGCCTGATGTTCTGAACTTTCAGCTTAAGCGATATGTTTTTCTTCCAAAGACTACAACCAAGAAGAAAATTACTTCTGCATTTAGTTTTCCTGGAGTACTTGATATGAGGAAGAGGCTATCTGACCCGTCTCAGGGTGAATCAGTATATGACTTGTCAGCTGTGCTGATTCACAAGGGAACTGCAGTTAACAGTGGCCACTATGTAGCGCATGTCAAGGATGAGAAGACAGGGCAATGGTGGGAATTTGACGATGAGCAGGTCTCAAACTTGGGTTCTCATCCGTTTGGAGAAGGGACTTCAAATTCCAATACCAAGCCTGTTAAACCGGAACCTGTTGATCGATCTTGCATGGAACAAAAGAATGCTATTTCAAATGGTGATAATGTGGATGTCATTAACCAACAACCTTCAGAATCTATTACCAGTCATGTAGAGACATTTTCATGTGCTGATGCATATATGCTGATGTATAGTCTCAGTCGTTCCCGGAAGGGTGAAGAGAAAGTGCATGTGGAATGTAATGCCAATAATAGGAAGATACAAGGTGATACTGTTTGTAGTTCTCTTCCATCTCATCTTTGTGATGAGATTGAAAGTTTTAATGCATCATATCTTGATGCTTGTGAGCAATACAAATTTAAGAAAGAAGAAGAGATGAATAATATCACAGAACGAAGACAGGAAGTGCGCTCAATTCTGTCGGAAGCTCCTGTTCAACAACTTGAGGAAACCTTTTTTTGGATATCAACAGACTGGCTTCGTCAGTGGGCTGATAACATGATTTCACCTGCTCTTGACAACACATCCATCCAATGTTCGCATGGAAAAGTTCCAGTTTCAAAGGTTGGCCACATGAAAAGGTTGTCAGTCAAAGCATGGACCAAACTGTTCTCTAAGTATAAAGGGGGGCCAGAACTGGCCAATGATGACTACTGCATGGTTTGCCTTATTGATGGTGCTCGAAATGTAGTCTGTGCTGACAGTTATAGGGATAGAAGAATAATAATGAAGCAAGTTGCAGAGGATGCACTTGCAGCGAAGTGTTCAGGTGGAATATATTATGTTTCTAAATCATGGTTACAACAGTGGCTTAAAAGAAAAATTCTTGATGCTCCAGCTGAAGCCGATGCCGGACCTACAGCTTCAATTAGGTGTCCTCATGGGGAACTTATGCCTGAACACGCCACTGGTGCTAAGAGATTGCTTGTTCCTGAGAATCTCTGGCTCTTCCTTTATGAAGATGCTTTAACAGTGAAGCCTGATGATCATTTGGGTTGTTTTCCATTTCCTTCTGACTCTGCACAGTGTTCCCAATGCAGTGATGAGCTATCTGAAGTTGCATGTATGGAGGATTCTTTAAGATTGGTGAGGCTCAAACAGCGCCAGACTCATGAGAAACTACTTACGGGAAAAAGTGTGCCACTTTCTCTGCATTGCAAGTATTACTTGATACCCAATTCTTGGCTTTCAAAGTGGAAAAACTACATTACTGCAAATGGAAAGAATATTTCCTCAGTGGAGAAACCCGAAACATTAGACGGCATCATGGATTTGCTAAAGTGTGAAAAGCATTCACAGCTTCTAGAAAGGCCTGTTGATCTGGTCCAGAAACGTGGTCTGATTTCCCAGAAGTCTCCTGTGGATGGGTTAATACTCATTACTGAGAATGACTGGAAGTCCTTCTGTGAGGAATGGGGCGGTATTGAAGAGAAAGGAATATCTGCTGAAATAGAGTTAAGTAGTACTGAAGAAAATAATTTGGCAGGGTCTTGCGAAGAGATGCCAATGGGCGAGGAGGACCTCAGTACTCCAAATCACGTAAATGGTGAAGTTGAGTGTAGACGACTTATGATTAAGACTTGTCCTGAGATATGTGAGGACTGCATTGGAGAAAGAGAAAGCAGGGAATTAATGCGGAAGCTTGATTATTGCAACGAGGACATATATGTATACTTTGTACATGGCAAGGAAGCTCCAAAGTCGATTCTAAAACCATCTCAAACAAATTTCGATCCAGATCGCCGAGTTTCCAAGCGCTCTCGGAGGACAAAAACTGGGGATCAAATAAGCTTAAAAGTCTCTGGTTCCACAACCATATACCAGTTGAAAATGATGATATGGGAATCTTTTGGGATTGTTAAGGAAAACCAGGGACTTCACAAAGGTACTCGGATAATTGAGGACGAATCTGCTACTCTTGCGGACATGAATATCTTCCCTGGTGATAGACTTTGGGTGAATGATTCAGAAATCCATGAGAACCGAGATATTGCTGATGAGCTATCTGACCAGAAAATGGATGCGCAGCACACTGAAGAGGGGTTTCGCGGGACGCTGTTGACAACAAATGTTTCGTCCCAAGTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0007275 GO:0008150 GO:0009791 GO:0010154 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUSP UCH

Protein Analysis

1085

Amino Acids

122.12

Weight (kDa)

5.58

Isoelectric Point (pI)

51.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	107 - 452	5.4e-53	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	107 - 382	2.3e-18	Ubiquitin carboxyl-terminal hydrolase
DUSP	PF06337	538 - 605	1.2e-06	DUSP domain
DUSP	PF06337	775 - 863	7.5e-07	DUSP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49600 AT3G49600 AT3G49600
fragaria_vesca	FvH4_2g30650 FvH4_2g30650 FvH4_2g30650 FvH4_2g30650
malus_domestica	MD08G1133600.v1.1 MD15G1110500.v1.1
prunus_persica	Prupe.1G462800_v2.0.a1
pyrus_communis	pycom08g11240 pycom15g10080
rosa_chinensis	RchiOBHm_Chr6g0312261
rosa_laevigata	RLG00000010338
rosa_roxburghii	Rroxscaffold_7G00156900
rosa_rugosa	Rorug06G0394400
rosa_samantha	Rh6AG511600 Rh6BG522700 Rh6CG528400 Rh6DG513800
rosa_wichuraiana	Rw6G044500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1681
Acc16I	TGCGCA	2 cut(s)	878, 3189
Acc36I	ACCTGC	2 cut(s)	1132, 1681
AccB7I	CCANNNNNTGG	2 cut(s)	2346, 3181
AccI	GTMKAC	2 cut(s)	2725, 2835
AccII	CGCG	2 cut(s)	19, 3213
AciI	CCGC	4 cut(s)	2583, 2800, 3086, 3213
AclI	AACGTT	1 cut(s)	595
AclWI	GGATC	3 cut(s)	275, 2891, 2952
AcoI	YGGCCR	3 cut(s)	1078, 1732, 1803
AcsI	RAATTY	7 cut(s)	135, 169, 1127, 1186, 1520, 1986, 2890
AcuI	CTGAAG	6 cut(s)	723, 1278, 2025, 2238, 2652, 3219
AdeI	CACNNNGTG	1 cut(s)	689
AfaI	GTAC	7 cut(s)	16, 377, 981, 2629, 2692, 2844, 3052
AfeI	AGCGCT	1 cut(s)	2921
AfiI	CCNNNNNNNGG	8 cut(s)	218, 1015, 1016, 1416, 1623, 2346, 2528, 3181
AflII	CTTAAG	1 cut(s)	908
AflIII	ACRYGT	1 cut(s)	667
AhdI	GACNNNNNGTC	1 cut(s)	2560
AjnI	CCWGG	3 cut(s)	973, 3033, 3103
Alw21I	GWGCWC	3 cut(s)	683, 807, 1846
Alw26I	GTCTC	6 cut(s)	1017, 1150, 1313, 1364, 2523, 2968
AlwI	GGATC	3 cut(s)	275, 2891, 2952
Aor51HI	AGCGCT	1 cut(s)	2921
AoxI	GGCC	8 cut(s)	235, 274, 322, 1078, 1732, 1793, 1803, 2474
ApaI	GGGCCC	1 cut(s)	278
ApeKI	GCWGC	4 cut(s)	159, 436, 1919, 3190
ApoI	RAATTY	7 cut(s)	135, 169, 1127, 1186, 1520, 1986, 2890
ArsI	GACNNNNNNTTYG	1 cut(s)	3224
AseI	ATTAAT	1 cut(s)	2795
Asp700I	GAANNNNTTC	2 cut(s)	2564, 3098
AspLEI	GCGC	7 cut(s)	21, 879, 1093, 1575, 2268, 2922, 3190
AspS9I	GGNCC	8 cut(s)	274, 275, 292, 1764, 1793, 2018, 2488, 2683
AsuC2I	CCSGG	1 cut(s)	1372
AvaII	GGWCC	5 cut(s)	292, 1764, 2018, 2488, 2683
AxyI	CCTNAGG	1 cut(s)	420
BaeGI	GKGCMC	1 cut(s)	278
BalI	TGGCCA	3 cut(s)	1080, 1734, 1805
BanII	GRGCYC	2 cut(s)	278, 807
BbsI	GAAGAC	4 cut(s)	254, 729, 1115, 1567
Bbv12I	GWGCWC	3 cut(s)	683, 807, 1846
BbvI	GCAGC	4 cut(s)	146, 448, 1931, 3202
BccI	CCATC	5 cut(s)	1462, 1700, 1832, 2522, 2887
BceAI	ACGGC	2 cut(s)	147, 2440
BcgI	CGANNNNNNTGC	2 cut(s)	299, 333
BciT130I	CCWGG	3 cut(s)	975, 3035, 3105
BclI	TGATCA	1 cut(s)	2149
BcnI	CCSGG	1 cut(s)	1372
BcoDI	GTCTC	6 cut(s)	1017, 1150, 1313, 1364, 2523, 2968
BfaI	CTAG	2 cut(s)	675, 2468
BfmI	CTRYAG	3 cut(s)	318, 1065, 2022
BfoI	RGCGCY	2 cut(s)	2269, 2923
BfrI	CTTAAG	1 cut(s)	908
BfuAI	ACCTGC	2 cut(s)	1132, 1681
BglII	AGATCT	1 cut(s)	643
BisI	GCNGC	4 cut(s)	160, 437, 1920, 3191
BlsI	GCNGC	4 cut(s)	161, 438, 1921, 3192
BmcAI	AGTACT	3 cut(s)	981, 2629, 2692
Bme1390I	CCNGG	4 cut(s)	975, 1372, 3035, 3105
Bme18I	GGWCC	5 cut(s)	292, 1764, 2018, 2488, 2683
BmeRI	GACNNNNNGTC	1 cut(s)	2560
BmgT120I	GGNCC	8 cut(s)	274, 275, 292, 1764, 1793, 2018, 2488, 2683
BmiI	GGNNCC	4 cut(s)	276, 1215, 1794, 2971
BmrFI	CCNGG	4 cut(s)	975, 1372, 3035, 3105
BmrI	ACTGGG	1 cut(s)	2949
BmuI	ACTGGG	1 cut(s)	2949
BoxI	GACNNNNGTC	1 cut(s)	297
BpiI	GAAGAC	4 cut(s)	254, 729, 1115, 1567
BplI	GAGNNNNNCTC	2 cut(s)	1130, 1162
BpmI	CTGGAG	3 cut(s)	548, 996, 1983
BpuEI	CTTGAG	1 cut(s)	1628
BpuMI	CCSGG	1 cut(s)	1372
Bsa29I	ATCGAT	1 cut(s)	1225
BsaAI	YACGTR	1 cut(s)	2704
BsaBI	GATNNNNATC	1 cut(s)	2901
BsaI	GGTCTC	1 cut(s)	1150
BsaJI	CCNNGG	4 cut(s)	652, 808, 3034, 3103
BsaWI	WCCGGW	1 cut(s)	1210
BsaXI	ACNNNNNCTCC	2 cut(s)	39, 69
Bsc4I	CCNNNNNNNGG	8 cut(s)	218, 1015, 1016, 1416, 1623, 2346, 2528, 3181
Bse21I	CCTNAGG	1 cut(s)	420
Bse3DI	GCAATG	3 cut(s)	881, 1124, 2317
Bse8I	GATNNNNATC	1 cut(s)	2901
BseBI	CCWGG	3 cut(s)	975, 3035, 3105
BseCI	ATCGAT	1 cut(s)	1225
BseDI	CCNNGG	4 cut(s)	652, 808, 3034, 3103
BseGI	GGATG	8 cut(s)	1108, 1162, 1279, 1688, 1692, 1915, 2533, 3190
BseJI	GATNNNNATC	1 cut(s)	2901
BseLI	CCNNNNNNNGG	8 cut(s)	218, 1015, 1016, 1416, 1623, 2346, 2528, 3181
BseMI	GCAATG	3 cut(s)	881, 1124, 2317
BseRI	GAGGAG	1 cut(s)	2693
BseSI	GKGCMC	1 cut(s)	278
BseXI	GCAGC	4 cut(s)	146, 448, 1931, 3202
BsgI	GTGCAG	1 cut(s)	2169
Bsh1236I	CGCG	2 cut(s)	19, 3213
BshFI	GGCC	8 cut(s)	237, 276, 324, 1080, 1734, 1795, 1805, 2476
BshVI	ATCGAT	1 cut(s)	1225
BsiHKAI	GWGCWC	3 cut(s)	683, 807, 1846
BsiSI	CCGG	3 cut(s)	1211, 1372, 2016
BslFI	GGGAC	4 cut(s)	1192, 3051, 3226, 3229
BslI	CCNNNNNNNGG	8 cut(s)	218, 1015, 1016, 1416, 1623, 2346, 2528, 3181
BsmAI	GTCTC	6 cut(s)	1017, 1150, 1313, 1364, 2523, 2968
BsmBI	CGTCTC	1 cut(s)	1017
BsmFI	GGGAC	4 cut(s)	1192, 3051, 3226, 3229
BsmI	GAATGC	2 cut(s)	1252, 2455
BsnI	GGCC	8 cut(s)	237, 276, 324, 1080, 1734, 1795, 1805, 2476
Bso31I	GGTCTC	1 cut(s)	1150
Bsp120I	GGGCCC	1 cut(s)	274
Bsp1286I	GDGCHC	4 cut(s)	278, 683, 807, 1846
Bsp1407I	TGTACA	2 cut(s)	375, 2842
Bsp143I	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
Bsp19I	CCATGG	1 cut(s)	808
BspACI	CCGC	4 cut(s)	2583, 2800, 3086, 3213
BspANI	GGCC	8 cut(s)	237, 276, 324, 1080, 1734, 1795, 1805, 2476
BspDI	ATCGAT	1 cut(s)	1225
BspFNI	CGCG	2 cut(s)	19, 3213
BspHI	TCATGA	2 cut(s)	559, 2275
BspLI	GGNNCC	4 cut(s)	276, 1215, 1794, 2971
BspMAI	CTGCAG	2 cut(s)	322, 1069
BspMI	ACCTGC	2 cut(s)	1132, 1681
BspPI	GGATC	3 cut(s)	275, 2891, 2952
BspQI	GCTCTTC	2 cut(s)	795, 2117
BspTI	CTTAAG	1 cut(s)	908
BspTNI	GGTCTC	1 cut(s)	1150
BsrDI	GCAATG	3 cut(s)	881, 1124, 2317
BsrGI	TGTACA	2 cut(s)	375, 2842
BssECI	CCNNGG	4 cut(s)	652, 808, 3034, 3103
BssMI	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
BssNAI	GTATAC	1 cut(s)	2836
BssT1I	CCWWGG	1 cut(s)	808
Bst1107I	GTATAC	1 cut(s)	2836
Bst2UI	CCWGG	3 cut(s)	975, 3035, 3105
Bst4CI	ACNGT	8 cut(s)	557, 1076, 1438, 1773, 1868, 1971, 2137, 2190
Bst6I	CTCTTC	9 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2586, 2655, 3195
BstAFI	CTTAAG	1 cut(s)	908
BstAPI	GCANNNNNTGC	2 cut(s)	1827, 1910
BstAUI	TGTACA	2 cut(s)	375, 2842
BstBAI	YACGTR	1 cut(s)	2704
BstC8I	GCNNGC	2 cut(s)	322, 480
BstDSI	CCRYGG	2 cut(s)	652, 808
BstF5I	GGATG	8 cut(s)	1108, 1162, 1279, 1688, 1692, 1915, 2533, 3190
BstFNI	CGCG	2 cut(s)	19, 3213
BstH2I	RGCGCY	2 cut(s)	2269, 2923
BstHHI	GCGC	7 cut(s)	21, 879, 1093, 1575, 2268, 2922, 3190
BstKTI	GATC	9 cut(s)	283, 646, 1225, 1229, 2152, 2485, 2899, 2905, 2947
BstMAI	GTCTC	6 cut(s)	1017, 1150, 1313, 1364, 2523, 2968
BstMBI	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
BstMWI	GCNNNNNNNGC	9 cut(s)	460, 883, 1827, 1910, 1919, 2075, 2263, 2461, 3083
BstNI	CCWGG	3 cut(s)	975, 3035, 3105
BstNSI	RCATGY	7 cut(s)	92, 348, 482, 671, 1097, 1397, 2229
BstPAI	GACNNNNGTC	1 cut(s)	297
BstSCI	CCNGG	4 cut(s)	973, 1370, 3033, 3103
BstSFI	CTRYAG	3 cut(s)	318, 1065, 2022
BstSLI	GKGCMC	1 cut(s)	278
BstUI	CGCG	2 cut(s)	19, 3213
BstV1I	GCAGC	4 cut(s)	146, 448, 1931, 3202
BstV2I	GAAGAC	4 cut(s)	254, 729, 1115, 1567
BstX2I	RGATCY	2 cut(s)	280, 643
BstXI	CCANNNNNNTGG	2 cut(s)	490, 2498
BstYI	RGATCY	2 cut(s)	280, 643
BstZ17I	GTATAC	1 cut(s)	2836
Bsu15I	ATCGAT	1 cut(s)	1225
Bsu36I	CCTNAGG	1 cut(s)	420
BsuRI	GGCC	8 cut(s)	237, 276, 324, 1080, 1734, 1795, 1805, 2476
BsuTUI	ATCGAT	1 cut(s)	1225
BtgI	CCRYGG	2 cut(s)	652, 808
BtsCI	GGATG	8 cut(s)	1108, 1162, 1279, 1688, 1692, 1915, 2533, 3190
BtsI	GCAGTG	1 cut(s)	2209
BveI	ACCTGC	2 cut(s)	1132, 1681
Cac8I	GCNNGC	2 cut(s)	322, 480
CciI	TCATGA	2 cut(s)	559, 2275
CfoI	GCGC	7 cut(s)	21, 879, 1093, 1575, 2268, 2922, 3190
Cfr13I	GGNCC	8 cut(s)	274, 275, 292, 1764, 1793, 2018, 2488, 2683
ClaI	ATCGAT	1 cut(s)	1225
CseI	GACGC	1 cut(s)	3226
Csp6I	GTAC	7 cut(s)	15, 376, 980, 2628, 2691, 2843, 3051
CviQI	GTAC	7 cut(s)	15, 376, 980, 2628, 2691, 2843, 3051
DpnI	GATC	9 cut(s)	282, 645, 1224, 1228, 2151, 2484, 2898, 2904, 2946
DpnII	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
DraIII	CACNNNGTG	1 cut(s)	689
DriI	GACNNNNNGTC	1 cut(s)	2560
EaeI	YGGCCR	3 cut(s)	1078, 1732, 1803
Eam1104I	CTCTTC	9 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2586, 2655, 3195
Eam1105I	GACNNNNNGTC	1 cut(s)	2560
EarI	CTCTTC	9 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2586, 2655, 3195
Ecl136II	GAGCTC	1 cut(s)	805
Eco130I	CCWWGG	1 cut(s)	808
Eco147I	AGGCCT	3 cut(s)	237, 324, 2476
Eco24I	GRGCYC	2 cut(s)	278, 807
Eco31I	GGTCTC	1 cut(s)	1150
Eco32I	GATATC	1 cut(s)	1629
Eco47I	GGWCC	5 cut(s)	292, 1764, 2018, 2488, 2683
Eco47III	AGCGCT	1 cut(s)	2921
Eco53kI	GAGCTC	1 cut(s)	805
Eco57I	CTGAAG	6 cut(s)	723, 1278, 2025, 2238, 2652, 3219
Eco81I	CCTNAGG	1 cut(s)	420
EcoICRI	GAGCTC	1 cut(s)	805
EcoO109I	RGGNCCY	2 cut(s)	292, 2683
EcoRI	GAATTC	1 cut(s)	169
EcoRII	CCWGG	3 cut(s)	973, 3033, 3103
EcoRV	GATATC	1 cut(s)	1629
EcoT14I	CCWWGG	1 cut(s)	808
EcoT22I	ATGCAT	2 cut(s)	1342, 1492
EcoT38I	GRGCYC	2 cut(s)	278, 807
ErhI	CCWWGG	1 cut(s)	808
Esp3I	CGTCTC	1 cut(s)	1017
FalI	AAGNNNNNCTT	2 cut(s)	2315, 2347
FaqI	GGGAC	4 cut(s)	1192, 3051, 3226, 3229
FauI	CCCGC	1 cut(s)	3206
FbaI	TGATCA	1 cut(s)	2149
FblI	GTMKAC	2 cut(s)	2725, 2835
Fnu4HI	GCNGC	4 cut(s)	160, 437, 1920, 3191
FokI	GGATG	8 cut(s)	1115, 1149, 1286, 1675, 1679, 1922, 2540, 3197
FriOI	GRGCYC	2 cut(s)	278, 807
Fsp4HI	GCNGC	4 cut(s)	160, 437, 1920, 3191
FspBI	CTAG	2 cut(s)	675, 2468
FspI	TGCGCA	2 cut(s)	878, 3189
GlaI	GCGC	7 cut(s)	20, 878, 1092, 1574, 2267, 2921, 3189
GluI	GCNGC	4 cut(s)	160, 437, 1920, 3191
GsuI	CTGGAG	3 cut(s)	548, 996, 1983
HaeII	RGCGCY	2 cut(s)	2269, 2923
HaeIII	GGCC	8 cut(s)	237, 276, 324, 1080, 1734, 1795, 1805, 2476
HapII	CCGG	3 cut(s)	1211, 1372, 2016
HgaI	GACGC	1 cut(s)	3226
HhaI	GCGC	7 cut(s)	21, 879, 1093, 1575, 2268, 2922, 3190
Hin6I	GCGC	7 cut(s)	19, 877, 1091, 1573, 2266, 2920, 3188
HinP1I	GCGC	7 cut(s)	19, 877, 1091, 1573, 2266, 2920, 3188
HincII	GTYRAC	3 cut(s)	58, 1072, 3225
HindII	GTYRAC	3 cut(s)	58, 1072, 3225
HindIII	AAGCTT	3 cut(s)	705, 2804, 2953
HpaI	GTTAAC	1 cut(s)	1072
HpaII	CCGG	3 cut(s)	1211, 1372, 2016
Hpy166II	GTNNAC	5 cut(s)	58, 1072, 2726, 2836, 3225
Hpy8I	GTNNAC	5 cut(s)	58, 1072, 2726, 2836, 3225
HpyCH4III	ACNGT	8 cut(s)	557, 1076, 1438, 1773, 1868, 1971, 2137, 2190
HpyCH4IV	ACGT	3 cut(s)	595, 2497, 2703
HpyF10VI	GCNNNNNNNGC	9 cut(s)	460, 883, 1827, 1910, 1919, 2075, 2263, 2461, 3083
HpySE526I	ACGT	3 cut(s)	595, 2497, 2703
HspAI	GCGC	7 cut(s)	19, 877, 1091, 1573, 2266, 2920, 3188
Ksp22I	TGATCA	1 cut(s)	2149
KspAI	GTTAAC	1 cut(s)	1072
Kzo9I	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
LguI	GCTCTTC	2 cut(s)	795, 2117
LmnI	GCTCC	4 cut(s)	810, 1597, 2002, 2863
Lsp1109I	GCAGC	4 cut(s)	146, 448, 1931, 3202
MaeI	CTAG	2 cut(s)	675, 2468
MaeII	ACGT	3 cut(s)	595, 2497, 2703
MaeIII	GTNAC	2 cut(s)	670, 1962
MalI	GATC	9 cut(s)	282, 645, 1224, 1228, 2151, 2484, 2898, 2904, 2946
MboI	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
MflI	RGATCY	2 cut(s)	280, 643
MhlI	GDGCHC	4 cut(s)	278, 683, 807, 1846
MlsI	TGGCCA	3 cut(s)	1080, 1734, 1805
MluNI	TGGCCA	3 cut(s)	1080, 1734, 1805
MlyI	GAGTC	6 cut(s)	497, 692, 842, 858, 2174, 2266
MmeI	TCCRAC	2 cut(s)	717, 1566
Mox20I	TGGCCA	3 cut(s)	1080, 1734, 1805
Mph1103I	ATGCAT	2 cut(s)	1342, 1492
MroXI	GAANNNNTTC	2 cut(s)	2564, 3098
MscI	TGGCCA	3 cut(s)	1080, 1734, 1805
MslI	CAYNNNNRTG	3 cut(s)	1327, 1464, 2706
Msp20I	TGGCCA	3 cut(s)	1080, 1734, 1805
MspA1I	CMGCKG	2 cut(s)	1043, 2003
MspCI	CTTAAG	1 cut(s)	908
MspI	CCGG	3 cut(s)	1211, 1372, 2016
MspR9I	CCNGG	4 cut(s)	975, 1372, 3035, 3105
Mva1269I	GAATGC	2 cut(s)	1252, 2455
MvaI	CCWGG	3 cut(s)	975, 3035, 3105
MvnI	CGCG	2 cut(s)	19, 3213
MwoI	GCNNNNNNNGC	9 cut(s)	460, 883, 1827, 1910, 1919, 2075, 2263, 2461, 3083
NciI	CCSGG	1 cut(s)	1372
NcoI	CCATGG	1 cut(s)	808
NdeII	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
NlaIV	GGNNCC	4 cut(s)	276, 1215, 1794, 2971
NmeAIII	GCCGAG	2 cut(s)	439, 2933
NsbI	TGCGCA	2 cut(s)	878, 3189
NsiI	ATGCAT	2 cut(s)	1342, 1492
NspI	RCATGY	7 cut(s)	92, 348, 482, 671, 1097, 1397, 2229
PaeI	GCATGC	1 cut(s)	482
PagI	TCATGA	2 cut(s)	559, 2275
PaqCI	CACCTGC	1 cut(s)	1681
PceI	AGGCCT	3 cut(s)	237, 324, 2476
PciI	ACATGT	1 cut(s)	667
PciSI	GCTCTTC	2 cut(s)	795, 2117
PctI	GAATGC	2 cut(s)	1252, 2455
PdmI	GAANNNNTTC	2 cut(s)	2564, 3098
PflMI	CCANNNNNTGG	2 cut(s)	2346, 3181
PfoI	TCCNGGA	2 cut(s)	973, 1370
PkrI	GCNGC	4 cut(s)	161, 438, 1921, 3192
PleI	GAGTC	6 cut(s)	497, 692, 841, 857, 2174, 2266
PpsI	GAGTC	6 cut(s)	497, 692, 841, 857, 2174, 2266
Ppu21I	YACGTR	1 cut(s)	2704
PpuMI	RGGWCCY	2 cut(s)	292, 2683
PscI	ACATGT	1 cut(s)	667
PshAI	GACNNNNGTC	1 cut(s)	297
PshBI	ATTAAT	1 cut(s)	2795
Psp124BI	GAGCTC	1 cut(s)	807
Psp1406I	AACGTT	1 cut(s)	595
Psp5II	RGGWCCY	2 cut(s)	292, 2683
Psp6I	CCWGG	3 cut(s)	973, 3033, 3103
PspGI	CCWGG	3 cut(s)	973, 3033, 3103
PspN4I	GGNNCC	4 cut(s)	276, 1215, 1794, 2971
PspOMI	GGGCCC	1 cut(s)	274
PspPI	GGNCC	8 cut(s)	274, 275, 292, 1764, 1793, 2018, 2488, 2683
PspPPI	RGGWCCY	2 cut(s)	292, 2683
PstI	CTGCAG	2 cut(s)	322, 1069
PsuI	RGATCY	2 cut(s)	280, 643
PvuII	CAGCTG	2 cut(s)	1043, 2003
RsaI	GTAC	7 cut(s)	16, 377, 981, 2629, 2692, 2844, 3052
RsaNI	GTAC	7 cut(s)	15, 376, 980, 2628, 2691, 2843, 3051
RseI	CAYNNNNRTG	3 cut(s)	1327, 1464, 2706
SacI	GAGCTC	1 cut(s)	807
SapI	GCTCTTC	2 cut(s)	795, 2117
SatI	GCNGC	4 cut(s)	160, 437, 1920, 3191
Sau3AI	GATC	9 cut(s)	280, 643, 1222, 1226, 2149, 2482, 2896, 2902, 2944
Sau96I	GGNCC	8 cut(s)	274, 275, 292, 1764, 1793, 2018, 2488, 2683
SbfI	CCTGCAGG	1 cut(s)	322
ScaI	AGTACT	3 cut(s)	981, 2629, 2692
SchI	GAGTC	6 cut(s)	497, 692, 842, 858, 2174, 2266
ScrFI	CCNGG	4 cut(s)	975, 1372, 3035, 3105
SdaI	CCTGCAGG	1 cut(s)	322
SduI	GDGCHC	4 cut(s)	278, 683, 807, 1846
SfcI	CTRYAG	3 cut(s)	318, 1065, 2022
SinI	GGWCC	5 cut(s)	292, 1764, 2018, 2488, 2683
SmiMI	CAYNNNNRTG	3 cut(s)	1327, 1464, 2706
SmlI	CTYRAG	2 cut(s)	908, 1607
SmoI	CTYRAG	2 cut(s)	908, 1607
SphI	GCATGC	1 cut(s)	482
Sse8387I	CCTGCAGG	1 cut(s)	322
SseBI	AGGCCT	3 cut(s)	237, 324, 2476
SsiI	CCGC	4 cut(s)	2583, 2800, 3086, 3213
SspI	AATATT	3 cut(s)	75, 824, 2392
SspMI	CTAG	2 cut(s)	675, 2468
SstI	GAGCTC	1 cut(s)	807
StuI	AGGCCT	3 cut(s)	237, 324, 2476
StyD4I	CCNGG	4 cut(s)	973, 1370, 3033, 3103
StyI	CCWWGG	1 cut(s)	808
TaaI	ACNGT	8 cut(s)	557, 1076, 1438, 1773, 1868, 1971, 2137, 2190
TaiI	ACGT	3 cut(s)	598, 2500, 2706
TaqI	TCGA	6 cut(s)	852, 862, 1225, 1846, 2867, 2895
TatI	WGTACW	5 cut(s)	375, 979, 2627, 2690, 2842
TseI	GCWGC	4 cut(s)	159, 436, 1919, 3190
TspDTI	ATGAA	7 cut(s)	395, 1317, 1556, 1754, 1907, 2136, 3107
TspGWI	ACGGA	3 cut(s)	383, 641, 1155
Van91I	CCANNNNNTGG	2 cut(s)	2346, 3181
Vha464I	CTTAAG	1 cut(s)	908
VpaK11BI	GGWCC	5 cut(s)	292, 1764, 2018, 2488, 2683
VspI	ATTAAT	1 cut(s)	2795
XapI	RAATTY	7 cut(s)	135, 169, 1127, 1186, 1520, 1986, 2890
XbaI	TCTAGA	1 cut(s)	2467
XceI	RCATGY	7 cut(s)	92, 348, 482, 671, 1097, 1397, 2229
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1703
XmiI	GTMKAC	2 cut(s)	2725, 2835
XmnI	GAANNNNTTC	2 cut(s)	2564, 3098
XspI	CTAG	2 cut(s)	675, 2468
ZrmI	AGTACT	3 cut(s)	981, 2629, 2692
Zsp2I	ATGCAT	2 cut(s)	1342, 1492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.