Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010285

Physical Location & Seq

Genomic Coordinates Forward (+)

7733545 .. 7740719

Gene Structure 7175 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1110500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGAGCCGACCAAGTACACGCAGTAAAAATAAAAGAAACAGACAAGGAGATAATGTTGACAACACTTCCGAAATATTGAGGAAAATACATGCAACAGGTGAAATTACAAATGAGGATATATATATGCTGTATAAAATTTCAAAGCCAGTTTGTCAGGGCTGCCGTGTGAATACTAAAGATAACCCAAATTGCTTTTGTGGGCTAATTCCACCTCCTAATGGAAGTAGGAAATCAGGCTTATGGCAGAAGACATCAGAGATTCTGCAAACTCTTGGCCCGGACCCTTCTCAGGACCTTCGTCCTTCTGCCGATTCTCCTGCAGGCCTCACAAATCTTGGTGCAACATGCTATGCTAATAGCATACTCCAATGTTTGTACATGAACAAGTCCTTCCGGGAAGGTATCTTCTTGGTTGAACCTGAGGTTTTGGAACAGCAGCCAGTGTTGAATCAACTTTCTCGGCTTTTTGCACAGTTGCATGCCAGTAAATTGGCTTTTATAGACTCCTCTCCATTTGTAAAAACACTGGAGTTAGATAATGGAGTCCAGCAGGACAGTCATGAGTTTCTAACCTTACTTCTTTCTTTGCTTGAACGGTGTCTGAGCAACTCTAAAGTTTCAAAGGCAAAATCTATTGTTCAAGATCTTTTTCGTGGAAGTGTATCACATGTTACTAGGTGCTCACAATGTGGGAAAGACTCTGAAGCTTCTTCAAACATGGAAGACTTCTATGAGCTGGAGTTGAATGTGAAGGGTTTGAAAAGTTTGGATGAGAGTTTAGATGATTACCTTAGTGTTGAAGAGCTTCACGGAGAGAATCAATATTTTTGTGAGTCGTGTAAAGCAAGAGTCGATGCTACTCGAAGCATCAAGTTGCACACGCTGCCTGATGTTCTGAACTTTCAACTTAAGCGATGTGTTTTTCTTCCAAAGACGACAACCAAGAAGAAAATTACTTCCGCATTTAGTTTTCCTGGAGTACTTGATATGAGGAAGAGGCTATCTGAACCTTCTCAGGGTGAATCAATGTATGACTTGTCAGCTGTGCTGATTCACAAGGGAACTGCAGTTAACAGTGGCCACTATGTAGCGCATGTCAAGGATGAGAAGACAGGGCAATGGTGGGAATTCGACGATGAGCAAGTCTCAAACTTGGGTTCTCATCCGTTTGGAGAAGGGACTTCAAGTTCCAATACCAAGCCTGTTAAACCCGAACCTGTTAATCCATCTTGCACAGAACAAAAGAATGCTATTTCGAATGGTGATAATGTGGATGTCATTCACCAACAACTTTCAAAATCTATTACCAGTCATGTGGATACATTTTCATCTGGTGATGCATATATGCTGATGTATAATCTCAGGCGTACCCCGGAGGATGAAGAGAAAGGGTATGTGGAATGTAATGGCAATGATAGGAAGACAGAAGGTGATACTGTTTGTAGTTCTCTTCCATCTCATCTTTGTGATGAGATTGAAAGCTTTAATGCATCATATCTTGATGCTTGTGAGCAATACAAATTTAAGAAAGAAGAAGAGATGAATAATATCACAGAACGAAGACAGGAAGTGCGCTCAATTTTGTCTGAAGCTCCTGTTCAACAACTTGAGGAAACCTTTTTTTGGATATCGACAGACTGGCTTCGTCAATGGGCTGATAGCATGATTTCACCTGCTCTTGACAACACATCCATCCAATGTTCGCATGGAAAAGTTCCACTTTCAAAGATTGGCTACATGAAACGGTTGTCAGTTAAAGCATGGACCAAGCTGTTCTTTAAGTATAAAGGGGGGCCAGAACTGGCCAATGATGACTACTGCATGGTTTGCCTTATTGATGGTGCTCGAAATGTAGTTTGTGCTGACAGCTATAGGGATAGAAGAATAGTAATGAAGCAAGTTGGCGAGGATGCACTTGCAGGGAAGTGTTCAGATGGAATATATTATGTTTCTAAAGCATGGTTACAACAGTGGCTTAAAAGAAAAATTCTTGATGCTCCAGCTGAAGCTGATGCGGGACCAACAACTTCAATTAGGTGTCCTCATGGGCAACTTATGCCTGAGCAAGCCACTGGTGCTAAGAGATTGCTTGTTCCCGAGAATCTCTGGCTCTTCCTTTATAAGGATGCTTTAACAGTAAAGCCTGATGATCATTTGGGTTGTTTGCCTTTTCCTTCAGACTCTGCACAGTGTTCCCAATGCAATGATGAACTCTCTGAAGTTGCATGTATGGAGGATTCCTTAAGATTGGTGAGGCTCAAACAGCGCCAGAATCATGAAAAACTACTCACGGGAAAAAGTGTGCCACTTTCTCTGCATTGCAAATATTACTTAATACCCAGTTCTTGGCTTTCAAAGTGGAAAAACTACATTACTGCAAGTGGCAAGAATGTTTCCTCAGTGGAGAAACCTGAAACATTAGAGGGCATCATGGATTTGCTCAAGTGTGAAAAGCATTCACAACTTCTAGAAAGGCCTGTTGATCTGGTCCAGAAACGTGGTCTGATTTCCCAGAAGTCTCCTTCCGTGGATGGGTTAATATTCATTACTGAGAATGACTGGAAGTCCTTCTGTGAGGATTGGGGTGGTATTGAAGAGAAAGGCATATCTGCTGAAATAGAGTTAAGTAGTAATGAAGGAAATGATTTGGCAGGGTCCTGCGAAGGGATGCCAATGTGTGAGGAGGACCTCAGTACTCCAAATCACATAAACGGTGAAGTTGAGTCTAGACAACTTGTGATTAAGACTTGTCCTGAGATATGTGAGGACTGCATTGGAGAAAGAGAAAGCAGGGAATTAATGCGGAAGCTCGATTATTGCAATGAGGACATATATGTATACTTTGTACATGGAAAGGAAGCTCCAAAGTCAATTCTAAAACCATCTGAAACAAATTTTGATCCAGATCGCCGAGTTTCCAAGCGCTCTCGGAAGACAAGGACTGGGGATCAAATAAGTTTGAAAGTCTCTGGTTCCACAACCATATACCAGTTGAAAATGATGATATGGGAATCTTTTGGCATTGTTAAGGAAAACCAGGGACTTCACAAAGGTACTCGGGTCATTGAGGATGAATCTGCTACTCTTGCAGACATGAATATATTCCCCGGGGATAGACTTTGGGTGAATGATTCAGAAATCCATGAGAACCGAGATATTGCTGATGAGCTATCTGACCAGAAAATGGATGTGCAACACACCGAAGAGGGTTTTCGTGGGACGCTGTTGACAGCAAATGTTTCATCCCAAGTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0007275 GO:0008150 GO:0009791 GO:0010154 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUSP UCH

Protein Analysis

1086

Amino Acids

122.13

Weight (kDa)

5.45

Isoelectric Point (pI)

50.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	107 - 452	3.7e-53	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	107 - 382	9.7e-18	Ubiquitin carboxyl-terminal hydrolase
DUSP	PF06337	645 - 708	8.8e-06	DUSP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49600 AT3G49600 AT3G49600
fragaria_vesca	FvH4_2g30650 FvH4_2g30650 FvH4_2g30650 FvH4_2g30650
malus_domestica	MD08G1133600.v1.1 MD15G1110500.v1.1
prunus_persica	Prupe.1G462800_v2.0.a1
pyrus_communis	pycom08g11240 pycom15g10080
rosa_chinensis	RchiOBHm_Chr6g0312261
rosa_laevigata	RLG00000010338
rosa_roxburghii	Rroxscaffold_7G00156900
rosa_rugosa	Rorug06G0394400
rosa_samantha	Rh6AG511600 Rh6BG522700 Rh6CG528400 Rh6DG513800
rosa_wichuraiana	Rw6G044500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2121
AarI	CACCTGC	1 cut(s)	1681
Acc36I	ACCTGC	1 cut(s)	1681
AccB7I	CCANNNNNTGG	2 cut(s)	2346, 3184
AccI	GTMKAC	1 cut(s)	2838
AciI	CCGC	3 cut(s)	960, 2015, 2803
AclWI	GGATC	2 cut(s)	2894, 2955
AcoI	YGGCCR	2 cut(s)	1078, 1803
AcsI	RAATTY	5 cut(s)	135, 1127, 1520, 1986, 2893
AcuI	CTGAAG	5 cut(s)	723, 1608, 2025, 2160, 2238
AdeI	CACNNNGTG	1 cut(s)	689
AfaI	GTAC	7 cut(s)	16, 377, 981, 1369, 2695, 2847, 3055
AfeI	AGCGCT	1 cut(s)	2924
AfiI	CCNNNNNNNGG	9 cut(s)	218, 289, 1016, 1623, 2122, 2346, 2532, 2664, 3184
AflII	CTTAAG	2 cut(s)	908, 2242
AflIII	ACRYGT	1 cut(s)	667
AhdI	GACNNNNNGTC	1 cut(s)	2563
AjnI	CCWGG	2 cut(s)	973, 3036
AloI	GAACNNNNNNTCC	2 cut(s)	1171, 1203
Alw21I	GWGCWC	2 cut(s)	683, 1846
Alw26I	GTCTC	3 cut(s)	1150, 2523, 2971
AlwI	GGATC	2 cut(s)	2894, 2955
AlwNI	CAGNNNCTG	1 cut(s)	2183
Ama87I	CYCGRG	3 cut(s)	2096, 3057, 3107
Aor51HI	AGCGCT	1 cut(s)	2924
AoxI	GGCC	6 cut(s)	274, 322, 1078, 1793, 1803, 2474
ApeKI	GCWGC	3 cut(s)	159, 436, 883
ApoI	RAATTY	5 cut(s)	135, 1127, 1520, 1986, 2893
AseI	ATTAAT	1 cut(s)	2798
Asp700I	GAANNNNTTC	3 cut(s)	804, 2567, 3101
AspLEI	GCGC	4 cut(s)	1093, 1575, 2268, 2925
AspS9I	GGNCC	9 cut(s)	275, 280, 292, 1764, 1793, 2018, 2488, 2655, 2686
AsuC2I	CCSGG	5 cut(s)	278, 395, 1373, 3108, 3109
AsuII	TTCGAA	1 cut(s)	1256
AvaI	CYCGRG	3 cut(s)	2096, 3057, 3107
AvaII	GGWCC	7 cut(s)	280, 292, 1764, 2018, 2488, 2655, 2686
AxyI	CCTNAGG	1 cut(s)	420
BaeI	ACNNNNGTAYC	2 cut(s)	3045, 3078
BalI	TGGCCA	2 cut(s)	1080, 1805
BbsI	GAAGAC	6 cut(s)	254, 729, 1115, 1427, 1567, 2939
Bbv12I	GWGCWC	2 cut(s)	683, 1846
BbvI	GCAGC	3 cut(s)	146, 448, 870
BccI	CCATC	7 cut(s)	1234, 1462, 1700, 1832, 1928, 2525, 2890
BceAI	ACGGC	1 cut(s)	147
BcgI	CGANNNNNNTGC	2 cut(s)	299, 333
BciT130I	CCWGG	2 cut(s)	975, 3038
BciVI	GTATCC	1 cut(s)	1312
BclI	TGATCA	1 cut(s)	2149
BcnI	CCSGG	5 cut(s)	278, 395, 1373, 3108, 3109
BcoDI	GTCTC	3 cut(s)	1150, 2523, 2971
BfaI	CTAG	3 cut(s)	675, 2468, 2727
BfmI	CTRYAG	3 cut(s)	318, 1065, 1870
BfoI	RGCGCY	2 cut(s)	2269, 2926
BfrI	CTTAAG	2 cut(s)	908, 2242
BfuAI	ACCTGC	1 cut(s)	1681
BfuI	GTATCC	1 cut(s)	1312
BglII	AGATCT	1 cut(s)	643
BisI	GCNGC	3 cut(s)	160, 437, 884
BlsI	GCNGC	3 cut(s)	161, 438, 885
BmcAI	AGTACT	2 cut(s)	981, 2695
Bme1390I	CCNGG	7 cut(s)	278, 395, 975, 1373, 3038, 3108, 3109
Bme18I	GGWCC	7 cut(s)	280, 292, 1764, 2018, 2488, 2655, 2686
BmeRI	GACNNNNNGTC	1 cut(s)	2563
BmeT110I	CYCGRG	3 cut(s)	2096, 3057, 3107
BmgT120I	GGNCC	9 cut(s)	275, 280, 292, 1764, 1793, 2018, 2488, 2655, 2686
BmiI	GGNNCC	5 cut(s)	282, 1794, 2019, 2656, 2974
BmrFI	CCNGG	7 cut(s)	278, 395, 975, 1373, 3038, 3108, 3109
BmrI	ACTGGG	2 cut(s)	2334, 2952
BmuI	ACTGGG	2 cut(s)	2334, 2952
BoxI	GACNNNNGTC	1 cut(s)	297
BpiI	GAAGAC	6 cut(s)	254, 729, 1115, 1427, 1567, 2939
BplI	GAGNNNNNCTC	2 cut(s)	1130, 1162
BpmI	CTGGAG	4 cut(s)	548, 758, 996, 1983
Bpu10I	CCTNAGC	1 cut(s)	2061
Bpu14I	TTCGAA	1 cut(s)	1256
BpuEI	CTTGAG	2 cut(s)	1628, 2426
BpuMI	CCSGG	5 cut(s)	278, 395, 1373, 3108, 3109
BsaBI	GATNNNNATC	1 cut(s)	2904
BsaJI	CCNNGG	6 cut(s)	1371, 2526, 3037, 3106, 3107, 3108
BsaXI	ACNNNNNCTCC	2 cut(s)	39, 69
Bsc4I	CCNNNNNNNGG	9 cut(s)	218, 289, 1016, 1623, 2122, 2346, 2532, 2664, 3184
Bse21I	CCTNAGG	1 cut(s)	420
Bse3DI	GCAATG	5 cut(s)	1124, 1417, 2209, 2317, 2827
Bse8I	GATNNNNATC	1 cut(s)	2904
BseBI	CCWGG	2 cut(s)	975, 3038
BseDI	CCNNGG	6 cut(s)	1371, 2526, 3037, 3106, 3107, 3108
BseJI	GATNNNNATC	1 cut(s)	2904
BseLI	CCNNNNNNNGG	9 cut(s)	218, 289, 1016, 1623, 2122, 2346, 2532, 2664, 3184
BseMI	GCAATG	5 cut(s)	1124, 1417, 2209, 2317, 2827
BseRI	GAGGAG	2 cut(s)	496, 2696
BseXI	GCAGC	3 cut(s)	146, 448, 870
BsgI	GTGCAG	1 cut(s)	2169
BshFI	GGCC	6 cut(s)	276, 324, 1080, 1795, 1805, 2476
BsiHKAI	GWGCWC	2 cut(s)	683, 1846
BsiHKCI	CYCGRG	3 cut(s)	2096, 3057, 3107
BsiSI	CCGG	4 cut(s)	278, 394, 1373, 3108
BslFI	GGGAC	4 cut(s)	1192, 2031, 3054, 3232
BslI	CCNNNNNNNGG	9 cut(s)	218, 289, 1016, 1623, 2122, 2346, 2532, 2664, 3184
BsmAI	GTCTC	3 cut(s)	1150, 2523, 2971
BsmFI	GGGAC	4 cut(s)	1192, 2031, 3054, 3232
BsmI	GAATGC	2 cut(s)	1252, 2455
BsnI	GGCC	6 cut(s)	276, 324, 1080, 1795, 1805, 2476
BsoBI	CYCGRG	3 cut(s)	2096, 3057, 3107
Bsp119I	TTCGAA	1 cut(s)	1256
Bsp1286I	GDGCHC	2 cut(s)	683, 1846
Bsp1407I	TGTACA	2 cut(s)	375, 2845
Bsp143I	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
BspACI	CCGC	3 cut(s)	960, 2015, 2803
BspANI	GGCC	6 cut(s)	276, 324, 1080, 1795, 1805, 2476
BspHI	TCATGA	2 cut(s)	559, 2275
BspLI	GGNNCC	5 cut(s)	282, 1794, 2019, 2656, 2974
BspMAI	CTGCAG	2 cut(s)	322, 1069
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	2 cut(s)	2894, 2955
BspQI	GCTCTTC	2 cut(s)	795, 2117
BspT104I	TTCGAA	1 cut(s)	1256
BspTI	CTTAAG	2 cut(s)	908, 2242
BsrDI	GCAATG	5 cut(s)	1124, 1417, 2209, 2317, 2827
BsrGI	TGTACA	2 cut(s)	375, 2845
BssECI	CCNNGG	6 cut(s)	1371, 2526, 3037, 3106, 3107, 3108
BssMI	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
BssNAI	GTATAC	1 cut(s)	2839
Bst1107I	GTATAC	1 cut(s)	2839
Bst2UI	CCWGG	2 cut(s)	975, 3038
Bst6I	CTCTTC	8 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2589, 3198
BstAFI	CTTAAG	2 cut(s)	908, 2242
BstAPI	GCANNNNNTGC	3 cut(s)	883, 1827, 2056
BstAUI	TGTACA	2 cut(s)	375, 2845
BstBI	TTCGAA	1 cut(s)	1256
BstC8I	GCNNGC	3 cut(s)	322, 480, 2067
BstDSI	CCRYGG	1 cut(s)	2526
BstENI	CCTNNNNNAGG	1 cut(s)	2120
BstH2I	RGCGCY	2 cut(s)	2269, 2926
BstHHI	GCGC	4 cut(s)	1093, 1575, 2268, 2925
BstKTI	GATC	6 cut(s)	646, 2152, 2485, 2902, 2908, 2950
BstMAI	GTCTC	3 cut(s)	1150, 2523, 2971
BstMBI	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
BstMWI	GCNNNNNNNGC	6 cut(s)	883, 1827, 2056, 2075, 2263, 3086
BstNI	CCWGG	2 cut(s)	975, 3038
BstNSI	RCATGY	6 cut(s)	92, 348, 482, 671, 1097, 2229
BstPAI	GACNNNNGTC	1 cut(s)	297
BstSCI	CCNGG	7 cut(s)	276, 393, 973, 1371, 3036, 3106, 3107
BstSFI	CTRYAG	3 cut(s)	318, 1065, 1870
BstV1I	GCAGC	3 cut(s)	146, 448, 870
BstV2I	GAAGAC	6 cut(s)	254, 729, 1115, 1427, 1567, 2939
BstX2I	RGATCY	1 cut(s)	643
BstXI	CCANNNNNNTGG	3 cut(s)	490, 1315, 2498
BstYI	RGATCY	1 cut(s)	643
BstZ17I	GTATAC	1 cut(s)	2839
Bsu36I	CCTNAGG	1 cut(s)	420
BsuI	GTATCC	1 cut(s)	1312
BsuRI	GGCC	6 cut(s)	276, 324, 1080, 1795, 1805, 2476
BtgI	CCRYGG	1 cut(s)	2526
BtgZI	GCGATG	1 cut(s)	928
BtsIMutI	CAGTG	7 cut(s)	447, 524, 1081, 1976, 2070, 2195, 2406
BveI	ACCTGC	1 cut(s)	1681
Cac8I	GCNNGC	3 cut(s)	322, 480, 2067
CaiI	CAGNNNCTG	1 cut(s)	2183
CciI	TCATGA	2 cut(s)	559, 2275
CfoI	GCGC	4 cut(s)	1093, 1575, 2268, 2925
Cfr13I	GGNCC	9 cut(s)	275, 280, 292, 1764, 1793, 2018, 2488, 2655, 2686
Cfr9I	CCCGGG	1 cut(s)	3107
CseI	GACGC	1 cut(s)	3229
Csp6I	GTAC	7 cut(s)	15, 376, 980, 1368, 2694, 2846, 3054
CviQI	GTAC	7 cut(s)	15, 376, 980, 1368, 2694, 2846, 3054
DpnI	GATC	6 cut(s)	645, 2151, 2484, 2901, 2907, 2949
DpnII	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
DraIII	CACNNNGTG	1 cut(s)	689
DriI	GACNNNNNGTC	1 cut(s)	2563
EaeI	YGGCCR	2 cut(s)	1078, 1803
Eam1104I	CTCTTC	8 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2589, 3198
Eam1105I	GACNNNNNGTC	1 cut(s)	2563
EarI	CTCTTC	8 cut(s)	795, 989, 1377, 1455, 1530, 2117, 2589, 3198
Eco147I	AGGCCT	2 cut(s)	324, 2476
Eco32I	GATATC	1 cut(s)	1629
Eco47I	GGWCC	7 cut(s)	280, 292, 1764, 2018, 2488, 2655, 2686
Eco47III	AGCGCT	1 cut(s)	2924
Eco57I	CTGAAG	5 cut(s)	723, 1608, 2025, 2160, 2238
Eco81I	CCTNAGG	1 cut(s)	420
Eco88I	CYCGRG	3 cut(s)	2096, 3057, 3107
EcoNI	CCTNNNNNAGG	1 cut(s)	2120
EcoO109I	RGGNCCY	3 cut(s)	292, 2655, 2686
EcoRI	GAATTC	1 cut(s)	1127
EcoRII	CCWGG	2 cut(s)	973, 3036
EcoRV	GATATC	1 cut(s)	1629
EcoT22I	ATGCAT	2 cut(s)	1342, 1492
FalI	AAGNNNNNCTT	4 cut(s)	1704, 1736, 1760, 1792
FaqI	GGGAC	4 cut(s)	1192, 2031, 3054, 3232
FauI	CCCGC	1 cut(s)	2008
FbaI	TGATCA	1 cut(s)	2149
FblI	GTMKAC	1 cut(s)	2838
Fnu4HI	GCNGC	3 cut(s)	160, 437, 884
Fsp4HI	GCNGC	3 cut(s)	160, 437, 884
FspBI	CTAG	3 cut(s)	675, 2468, 2727
GlaI	GCGC	4 cut(s)	1092, 1574, 2267, 2924
GluI	GCNGC	3 cut(s)	160, 437, 884
GsuI	CTGGAG	4 cut(s)	548, 758, 996, 1983
HaeII	RGCGCY	2 cut(s)	2269, 2926
HaeIII	GGCC	6 cut(s)	276, 324, 1080, 1795, 1805, 2476
HapII	CCGG	4 cut(s)	278, 394, 1373, 3108
HgaI	GACGC	1 cut(s)	3229
HhaI	GCGC	4 cut(s)	1093, 1575, 2268, 2925
Hin6I	GCGC	4 cut(s)	1091, 1573, 2266, 2923
HinP1I	GCGC	4 cut(s)	1091, 1573, 2266, 2923
HincII	GTYRAC	3 cut(s)	58, 1072, 3228
HindII	GTYRAC	3 cut(s)	58, 1072, 3228
HindIII	AAGCTT	2 cut(s)	705, 1480
HpaI	GTTAAC	1 cut(s)	1072
HpaII	CCGG	4 cut(s)	278, 394, 1373, 3108
Hpy166II	GTNNAC	5 cut(s)	17, 58, 1072, 2839, 3228
Hpy8I	GTNNAC	5 cut(s)	17, 58, 1072, 2839, 3228
Hpy99I	CGWCG	1 cut(s)	1136
HpyCH4IV	ACGT	1 cut(s)	2497
HpyF10VI	GCNNNNNNNGC	6 cut(s)	883, 1827, 2056, 2075, 2263, 3086
HpySE526I	ACGT	1 cut(s)	2497
HspAI	GCGC	4 cut(s)	1091, 1573, 2266, 2923
Ksp22I	TGATCA	1 cut(s)	2149
KspAI	GTTAAC	1 cut(s)	1072
Kzo9I	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
LguI	GCTCTTC	2 cut(s)	795, 2117
LmnI	GCTCC	3 cut(s)	1597, 2002, 2866
Lsp1109I	GCAGC	3 cut(s)	146, 448, 870
MaeI	CTAG	3 cut(s)	675, 2468, 2727
MaeII	ACGT	1 cut(s)	2497
MaeIII	GTNAC	2 cut(s)	670, 1962
MalI	GATC	6 cut(s)	645, 2151, 2484, 2901, 2907, 2949
MboI	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
MflI	RGATCY	1 cut(s)	643
MhlI	GDGCHC	2 cut(s)	683, 1846
MlsI	TGGCCA	2 cut(s)	1080, 1805
MluNI	TGGCCA	2 cut(s)	1080, 1805
MlyI	GAGTC	7 cut(s)	497, 552, 692, 842, 858, 2174, 2732
Mox20I	TGGCCA	2 cut(s)	1080, 1805
Mph1103I	ATGCAT	2 cut(s)	1342, 1492
MroXI	GAANNNNTTC	3 cut(s)	804, 2567, 3101
MscI	TGGCCA	2 cut(s)	1080, 1805
MslI	CAYNNNNRTG	1 cut(s)	1464
Msp20I	TGGCCA	2 cut(s)	1080, 1805
MspA1I	CMGCKG	2 cut(s)	1043, 2003
MspCI	CTTAAG	2 cut(s)	908, 2242
MspI	CCGG	4 cut(s)	278, 394, 1373, 3108
MspR9I	CCNGG	7 cut(s)	278, 395, 975, 1373, 3038, 3108, 3109
Mva1269I	GAATGC	2 cut(s)	1252, 2455
MvaI	CCWGG	2 cut(s)	975, 3038
MwoI	GCNNNNNNNGC	6 cut(s)	883, 1827, 2056, 2075, 2263, 3086
NciI	CCSGG	5 cut(s)	278, 395, 1373, 3108, 3109
NdeII	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
NlaIV	GGNNCC	5 cut(s)	282, 1794, 2019, 2656, 2974
NmeAIII	GCCGAG	2 cut(s)	439, 2936
NsiI	ATGCAT	2 cut(s)	1342, 1492
NspI	RCATGY	6 cut(s)	92, 348, 482, 671, 1097, 2229
NspV	TTCGAA	1 cut(s)	1256
PaeI	GCATGC	1 cut(s)	482
PagI	TCATGA	2 cut(s)	559, 2275
PaqCI	CACCTGC	1 cut(s)	1681
PceI	AGGCCT	2 cut(s)	324, 2476
PciI	ACATGT	1 cut(s)	667
PciSI	GCTCTTC	2 cut(s)	795, 2117
PctI	GAATGC	2 cut(s)	1252, 2455
PdmI	GAANNNNTTC	3 cut(s)	804, 2567, 3101
PflMI	CCANNNNNTGG	2 cut(s)	2346, 3184
PfoI	TCCNGGA	2 cut(s)	393, 973
PkrI	GCNGC	3 cut(s)	161, 438, 885
PleI	GAGTC	7 cut(s)	497, 551, 692, 841, 857, 2174, 2731
PpsI	GAGTC	7 cut(s)	497, 551, 692, 841, 857, 2174, 2731
PpuMI	RGGWCCY	3 cut(s)	292, 2655, 2686
PscI	ACATGT	1 cut(s)	667
PshAI	GACNNNNGTC	1 cut(s)	297
PshBI	ATTAAT	1 cut(s)	2798
PsiI	TTATAA	1 cut(s)	2121
Psp5II	RGGWCCY	3 cut(s)	292, 2655, 2686
Psp6I	CCWGG	2 cut(s)	973, 3036
PspGI	CCWGG	2 cut(s)	973, 3036
PspN4I	GGNNCC	5 cut(s)	282, 1794, 2019, 2656, 2974
PspPI	GGNCC	9 cut(s)	275, 280, 292, 1764, 1793, 2018, 2488, 2655, 2686
PspPPI	RGGWCCY	3 cut(s)	292, 2655, 2686
PstI	CTGCAG	2 cut(s)	322, 1069
PstNI	CAGNNNCTG	1 cut(s)	2183
PsuI	RGATCY	1 cut(s)	643
PvuII	CAGCTG	2 cut(s)	1043, 2003
RsaI	GTAC	7 cut(s)	16, 377, 981, 1369, 2695, 2847, 3055
RsaNI	GTAC	7 cut(s)	15, 376, 980, 1368, 2694, 2846, 3054
RseI	CAYNNNNRTG	1 cut(s)	1464
SapI	GCTCTTC	2 cut(s)	795, 2117
SatI	GCNGC	3 cut(s)	160, 437, 884
Sau3AI	GATC	6 cut(s)	643, 2149, 2482, 2899, 2905, 2947
Sau96I	GGNCC	9 cut(s)	275, 280, 292, 1764, 1793, 2018, 2488, 2655, 2686
SbfI	CCTGCAGG	1 cut(s)	322
ScaI	AGTACT	2 cut(s)	981, 2695
SchI	GAGTC	7 cut(s)	497, 552, 692, 842, 858, 2174, 2732
ScrFI	CCNGG	7 cut(s)	278, 395, 975, 1373, 3038, 3108, 3109
SdaI	CCTGCAGG	1 cut(s)	322
SduI	GDGCHC	2 cut(s)	683, 1846
SfcI	CTRYAG	3 cut(s)	318, 1065, 1870
SfuI	TTCGAA	1 cut(s)	1256
SinI	GGWCC	7 cut(s)	280, 292, 1764, 2018, 2488, 2655, 2686
SmaI	CCCGGG	1 cut(s)	3109
SmiMI	CAYNNNNRTG	1 cut(s)	1464
SmlI	CTYRAG	4 cut(s)	908, 1607, 2242, 2441
SmoI	CTYRAG	4 cut(s)	908, 1607, 2242, 2441
SphI	GCATGC	1 cut(s)	482
Sse8387I	CCTGCAGG	1 cut(s)	322
SseBI	AGGCCT	2 cut(s)	324, 2476
SsiI	CCGC	3 cut(s)	960, 2015, 2803
SspI	AATATT	4 cut(s)	75, 824, 2327, 2541
SspMI	CTAG	3 cut(s)	675, 2468, 2727
StuI	AGGCCT	2 cut(s)	324, 2476
StyD4I	CCNGG	7 cut(s)	276, 393, 973, 1371, 3036, 3106, 3107
TaiI	ACGT	1 cut(s)	2500
TaqI	TCGA	7 cut(s)	852, 862, 1131, 1256, 1631, 1846, 2811
TatI	WGTACW	5 cut(s)	14, 375, 979, 2693, 2845
TscAI	CASTG	7 cut(s)	447, 531, 1081, 1976, 2077, 2195, 2406
TseI	GCWGC	3 cut(s)	159, 436, 883
TspGWI	ACGGA	3 cut(s)	825, 1155, 2515
TspMI	CCCGGG	1 cut(s)	3107
TspRI	CASTG	7 cut(s)	447, 531, 1081, 1976, 2077, 2195, 2406
Van91I	CCANNNNNTGG	2 cut(s)	2346, 3184
Vha464I	CTTAAG	2 cut(s)	908, 2242
VpaK11BI	GGWCC	7 cut(s)	280, 292, 1764, 2018, 2488, 2655, 2686
VspI	ATTAAT	1 cut(s)	2798
XagI	CCTNNNNNAGG	1 cut(s)	2120
XapI	RAATTY	5 cut(s)	135, 1127, 1520, 1986, 2893
XbaI	TCTAGA	2 cut(s)	2467, 2726
XceI	RCATGY	6 cut(s)	92, 348, 482, 671, 1097, 2229
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1703
XmaI	CCCGGG	1 cut(s)	3107
XmiI	GTMKAC	1 cut(s)	2838
XmnI	GAANNNNTTC	3 cut(s)	804, 2567, 3101
XspI	CTAG	3 cut(s)	675, 2468, 2727
ZrmI	AGTACT	2 cut(s)	981, 2695
Zsp2I	ATGCAT	2 cut(s)	1342, 1492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.