Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007151

Physical Location & Seq

Genomic Coordinates Reverse (-)

16443923 .. 16447515

Gene Structure 3593 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1152800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGGCTCGTTCCTCAAATGGATCTCCGGCTTCGCGGCCTCCCCCGATTCCGATTTCTTCAAAACGTCGTCGTTCGCTGACTGGTCAACGGCGGAGTTGGGATTGGCGGTTGAGGATCGGCCGTCGGGGTCATTGTTTCGGAAGTTGTCGTTGCCCGAAGTTGGCCCTGGGTTCATCTTCGGAGGTAAATGCTTATAGGAGAAAGATTTTCTTCAACTATGAGAAACGCATAAGGTTGCGGAGTCCGCCGGAGAAGGTTTTTGAGTACTTTGCGTCTTCTAGTACGCCAGATGGAGAGTTACTTATGAGACCGGGAGATCTTATGAGGGCAATTGTTCCCGTCTTCCCTCCATCTGAGTCCAACCTTCTAAGAGATGGATATTTGAGAGGGGAAAGAATCCTAGTGATTTGTATATTTTTCTTTGTAACACTACTAAGCATCCCAGAATCAAGTTTCTCTGTTGCTTTTAAAATGTTTGACCTCGATGATAGTGGACAAATTGACAAGGAAGAGTTCAAGAAAGTGATGGCTTTGATGCGAGCCTGCAATAGACAGGGAGCTTACCACAGGGATGGACTTCGTACTGGGCTAAAAGTCAATGGTTCTGTAGAAGATGGAGGACTTGTGGAGTACTTTTTCGGTAATGATGGGAAGGATTCCCTTGAAATTGATAAATTTGTCAAATTTTTGAGAGATTTACAGGATGAAATGGTGAGGTTGGAGTTTTCCCACTATGATTATAAATTACGGGGAACCATATTGGCGAAGGACTTTGCTTTGTCCATGGTTGCTTCTGCTGACATGAGTCATCTAAGCAATTTGCTTGATCGGGTTGAGGAATTGAGCAATAAACCACATCTTAGAGATGTGCGCATCACATTTGAGGAGTTCAAAAACTTGGCCGAGCTTCGTAAAAAACTGCAGCCTTTTTCTTTGGCCCTTTTCAGCAATGGCAAAGCCAATGGCATGCTAACACGAGAAGATTTCCAATGTGCTGCTGCTCATGTATGCGGTGTATCTCTCACTGATAATGTAGTGGAAATCGTATTCCAATTGTTCGATTTTAACCAAGATGGAAATTTAAGCTTAGATGAGTTTGTTAGAGTTTTACAGCGATGGGGAATGGACGTTGCTCAAGAGGTGGAGACAGGAATCATGATTCATGACTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005758 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006839 GO:0006851 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0019866 GO:0030001 GO:0030003 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0032592 GO:0032991 GO:0034220 GO:0034702 GO:0034703 GO:0034704 GO:0036444 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046872 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051560 GO:0051561 GO:0051562 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060401 GO:0065007 GO:0065008 GO:0070013 GO:0070509 GO:0070588 GO:0070838 GO:0072503 GO:0072507 GO:0072511 GO:0098573 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098798 GO:0098800 GO:1902495 GO:1990246 GO:1990351 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

391

Amino Acids

44.5

Weight (kDa)

8.59

Isoelectric Point (pI)

52.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	155 - 178	4.8e-06	EF hand domain
EF-hand_5	PF13202	155 - 176	1.4e-06	EF hand
EF-hand_8	PF13833	321 - 372	8.5e-10	EF-hand domain pair
EF-hand_7	PF13499	321 - 370	2.1e-07	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32060 AT4G32060
fragaria_vesca	FvH4_2g28590 FvH4_2g28590
malus_domestica	MD08G1152800.v1.1 MD15G1129400.v1.1
prunus_persica	Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1
pyrus_communis	pycom08g13170 pycom15g11660
rosa_chinensis	RchiOBHm_Chr6g0297361
rosa_laevigata	RLG00000011608
rosa_multiflora	Rmu_ssc0000109.1_g000001
rosa_roxburghii	Rroxscaffold_7G00170610
rosa_rugosa	Rorug06G0270900 Rorug06G0271000
rosa_samantha	Rh6AG382400 Rh6BG390500 Rh6CG395600 Rh6DG382600
rosa_wichuraiana	Rw6G033310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	741
Acc16I	TGCGCA	1 cut(s)	872
AccII	CGCG	1 cut(s)	34
AciI	CCGC	6 cut(s)	34, 91, 106, 238, 245, 1011
AclWI	GGATC	2 cut(s)	28, 122
AcoI	YGGCCR	2 cut(s)	118, 900
AcsI	RAATTY	3 cut(s)	674, 683, 1078
AfaI	GTAC	4 cut(s)	266, 283, 583, 632
AfiI	CCNNNNNNNGG	1 cut(s)	160
AgsI	TTSAA	5 cut(s)	60, 214, 517, 665, 892
AjnI	CCWGG	1 cut(s)	165
AluBI	AGCT	3 cut(s)	560, 907, 1086
AluI	AGCT	3 cut(s)	560, 907, 1086
Alw26I	GTCTC	2 cut(s)	301, 1139
AlwI	GGATC	2 cut(s)	28, 122
AoxI	GGCC	5 cut(s)	35, 118, 162, 900, 936
ApeKI	GCWGC	3 cut(s)	922, 995, 998
ApoI	RAATTY	3 cut(s)	674, 683, 1078
AspLEI	GCGC	1 cut(s)	873
AspS9I	GGNCC	2 cut(s)	163, 937
AsuC2I	CCSGG	1 cut(s)	312
AsuHPI	GGTGA	1 cut(s)	724
BauI	CACGAG	1 cut(s)	975
BbsI	GAAGAC	2 cut(s)	267, 334
BbvI	GCAGC	3 cut(s)	934, 982, 985
BccI	CCATC	9 cut(s)	284, 358, 368, 520, 566, 608, 641, 1067, 1110
BceAI	ACGGC	2 cut(s)	104, 105
BciT130I	CCWGG	1 cut(s)	167
BcnI	CCSGG	1 cut(s)	312
BcoDI	GTCTC	2 cut(s)	301, 1139
BfaI	CTAG	2 cut(s)	279, 401
BfmI	CTRYAG	2 cut(s)	606, 920
BglII	AGATCT	1 cut(s)	316
BisI	GCNGC	4 cut(s)	35, 923, 996, 999
BlsI	GCNGC	4 cut(s)	36, 924, 997, 1000
BmcAI	AGTACT	2 cut(s)	266, 632
Bme1390I	CCNGG	2 cut(s)	167, 312
BmgT120I	GGNCC	2 cut(s)	163, 937
BmiI	GGNNCC	1 cut(s)	754
BmrFI	CCNGG	2 cut(s)	167, 312
BmrI	ACTGGG	1 cut(s)	594
BmsI	GCATC	3 cut(s)	447, 525, 882
BmuI	ACTGGG	1 cut(s)	594
BoxI	GACNNNNGTC	1 cut(s)	804
BpiI	GAAGAC	2 cut(s)	267, 334
BpuEI	CTTGAG	1 cut(s)	1119
BpuMI	CCSGG	1 cut(s)	312
BsaI	GGTCTC	1 cut(s)	301
BsaJI	CCNNGG	3 cut(s)	165, 166, 783
BsaXI	ACNNNNNCTCC	2 cut(s)	609, 639
Bsc4I	CCNNNNNNNGG	1 cut(s)	160
Bse1I	ACTGG	2 cut(s)	85, 589
Bse3DI	GCAATG	1 cut(s)	955
BseBI	CCWGG	1 cut(s)	167
BseDI	CCNNGG	3 cut(s)	165, 166, 783
BseGI	GGATG	3 cut(s)	438, 577, 709
BseLI	CCNNNNNNNGG	1 cut(s)	160
BseMI	GCAATG	1 cut(s)	955
BseMII	CTCAG	1 cut(s)	345
BseNI	ACTGG	2 cut(s)	85, 589
BseRI	GAGGAG	1 cut(s)	899
BseX3I	CGGCCG	1 cut(s)	118
BseXI	GCAGC	3 cut(s)	934, 982, 985
Bsh1236I	CGCG	1 cut(s)	34
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	5 cut(s)	37, 120, 164, 902, 938
BsiEI	CGRYCG	1 cut(s)	121
BsiSI	CCGG	3 cut(s)	26, 248, 311
BslI	CCNNNNNNNGG	1 cut(s)	160
BsmAI	GTCTC	2 cut(s)	301, 1139
BsnI	GGCC	5 cut(s)	37, 120, 164, 902, 938
Bso31I	GGTCTC	1 cut(s)	301
Bsp143I	GATC	4 cut(s)	20, 114, 316, 826
Bsp19I	CCATGG	1 cut(s)	783
BspACI	CCGC	6 cut(s)	34, 91, 106, 238, 245, 1011
BspANI	GGCC	5 cut(s)	37, 120, 164, 902, 938
BspCNI	CTCAG	1 cut(s)	346
BspFNI	CGCG	1 cut(s)	34
BspHI	TCATGA	2 cut(s)	1155, 1162
BspLI	GGNNCC	1 cut(s)	754
BspMAI	CTGCAG	1 cut(s)	924
BspPI	GGATC	2 cut(s)	28, 122
BspTNI	GGTCTC	1 cut(s)	301
BsrDI	GCAATG	1 cut(s)	955
BsrI	ACTGG	2 cut(s)	85, 589
BssECI	CCNNGG	3 cut(s)	165, 166, 783
BssMI	GATC	4 cut(s)	20, 114, 316, 826
BssSI	CACGAG	1 cut(s)	975
BssT1I	CCWWGG	1 cut(s)	783
Bst2BI	CACGAG	1 cut(s)	975
Bst2UI	CCWGG	1 cut(s)	167
Bst6I	CTCTTC	1 cut(s)	504
BstC8I	GCNNGC	3 cut(s)	540, 544, 968
BstDEI	CTNAG	6 cut(s)	354, 368, 434, 812, 860, 1087
BstDSI	CCRYGG	1 cut(s)	783
BstF5I	GGATG	3 cut(s)	438, 577, 709
BstFNI	CGCG	1 cut(s)	34
BstHHI	GCGC	1 cut(s)	873
BstKTI	GATC	4 cut(s)	23, 117, 319, 829
BstMAI	GTCTC	2 cut(s)	301, 1139
BstMBI	GATC	4 cut(s)	20, 114, 316, 826
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	1 cut(s)	244
BstNI	CCWGG	1 cut(s)	167
BstNSI	RCATGY	1 cut(s)	970
BstPAI	GACNNNNGTC	1 cut(s)	804
BstSCI	CCNGG	2 cut(s)	165, 310
BstSFI	CTRYAG	2 cut(s)	606, 920
BstUI	CGCG	1 cut(s)	34
BstV1I	GCAGC	3 cut(s)	934, 982, 985
BstV2I	GAAGAC	2 cut(s)	267, 334
BstX2I	RGATCY	2 cut(s)	20, 316
BstXI	CCANNNNNNTGG	1 cut(s)	572
BstYI	RGATCY	2 cut(s)	20, 316
BstZI	CGGCCG	1 cut(s)	118
BsuRI	GGCC	5 cut(s)	37, 120, 164, 902, 938
BtgI	CCRYGG	1 cut(s)	783
BtgZI	GCGATG	1 cut(s)	1129
BtsCI	GGATG	3 cut(s)	438, 577, 709
BtsIMutI	CAGTG	1 cut(s)	1023
Cac8I	GCNNGC	3 cut(s)	540, 544, 968
CciI	TCATGA	2 cut(s)	1155, 1162
CfoI	GCGC	1 cut(s)	873
Cfr13I	GGNCC	2 cut(s)	163, 937
CseI	GACGC	1 cut(s)	261
Csp6I	GTAC	4 cut(s)	265, 282, 582, 631
CviAII	CATG	6 cut(s)	784, 802, 967, 1004, 1156, 1163
CviQI	GTAC	4 cut(s)	265, 282, 582, 631
DdeI	CTNAG	6 cut(s)	354, 368, 434, 812, 860, 1087
DpnI	GATC	4 cut(s)	22, 116, 318, 828
DpnII	GATC	4 cut(s)	20, 114, 316, 826
DraI	TTTAAA	1 cut(s)	469
EaeI	YGGCCR	2 cut(s)	118, 900
EagI	CGGCCG	1 cut(s)	118
Eam1104I	CTCTTC	1 cut(s)	504
EarI	CTCTTC	1 cut(s)	504
EciI	GGCGGA	2 cut(s)	106, 234
EclXI	CGGCCG	1 cut(s)	118
Eco130I	CCWWGG	1 cut(s)	783
Eco31I	GGTCTC	1 cut(s)	301
Eco52I	CGGCCG	1 cut(s)	118
EcoRII	CCWGG	1 cut(s)	165
EcoT14I	CCWWGG	1 cut(s)	783
ErhI	CCWWGG	1 cut(s)	783
FaeI	CATG	6 cut(s)	787, 805, 970, 1007, 1159, 1166
FalI	AAGNNNNNCTT	2 cut(s)	194, 226
FatI	CATG	6 cut(s)	783, 801, 966, 1003, 1155, 1162
Fnu4HI	GCNGC	4 cut(s)	35, 923, 996, 999
FokI	GGATG	3 cut(s)	425, 584, 716
Fsp4HI	GCNGC	4 cut(s)	35, 923, 996, 999
FspAI	RTGCGCAY	1 cut(s)	872
FspBI	CTAG	2 cut(s)	279, 401
FspI	TGCGCA	1 cut(s)	872
GlaI	GCGC	1 cut(s)	872
GluI	GCNGC	4 cut(s)	35, 923, 996, 999
HaeIII	GGCC	5 cut(s)	37, 120, 164, 902, 938
HapII	CCGG	3 cut(s)	26, 248, 311
HgaI	GACGC	1 cut(s)	261
HhaI	GCGC	1 cut(s)	873
Hin1II	CATG	6 cut(s)	787, 805, 970, 1007, 1159, 1166
Hin6I	GCGC	1 cut(s)	871
HinP1I	GCGC	1 cut(s)	871
HincII	GTYRAC	1 cut(s)	86
HindII	GTYRAC	1 cut(s)	86
HindIII	AAGCTT	1 cut(s)	1084
HpaII	CCGG	3 cut(s)	26, 248, 311
HphI	GGTGA	1 cut(s)	724
Hpy166II	GTNNAC	2 cut(s)	86, 494
Hpy188I	TCNGA	4 cut(s)	51, 140, 181, 355
Hpy188III	TCNNGA	4 cut(s)	517, 1136, 1156, 1163
Hpy8I	GTNNAC	2 cut(s)	86, 494
Hpy99I	CGWCG	3 cut(s)	69, 72, 126
HpyAV	CCTTC	4 cut(s)	247, 374, 646, 760
HpyCH4IV	ACGT	2 cut(s)	64, 1128
HpyCH4V	TGCA	2 cut(s)	546, 922
HpyF10VI	GCNNNNNNNGC	1 cut(s)	244
HpyF3I	CTNAG	6 cut(s)	354, 368, 434, 812, 860, 1087
HpySE526I	ACGT	2 cut(s)	64, 1128
Hsp92II	CATG	6 cut(s)	787, 805, 970, 1007, 1159, 1166
HspAI	GCGC	1 cut(s)	871
Kzo9I	GATC	4 cut(s)	20, 114, 316, 826
LmnI	GCTCC	1 cut(s)	557
Lsp1109I	GCAGC	3 cut(s)	934, 982, 985
LweI	GCATC	3 cut(s)	447, 525, 882
MaeI	CTAG	2 cut(s)	279, 401
MaeII	ACGT	2 cut(s)	64, 1128
MaeIII	GTNAC	2 cut(s)	297, 424
MalI	GATC	4 cut(s)	22, 116, 318, 828
MboI	GATC	4 cut(s)	20, 114, 316, 826
MboII	GAAGA	8 cut(s)	48, 168, 202, 267, 334, 521, 623, 992
MfeI	CAATTG	2 cut(s)	330, 1052
MflI	RGATCY	2 cut(s)	20, 316
MlyI	GAGTC	4 cut(s)	250, 365, 814, 1160
MmeI	TCCRAC	2 cut(s)	384, 699
MseI	TTAA	3 cut(s)	468, 1065, 1082
MslI	CAYNNNNRTG	1 cut(s)	570
MspI	CCGG	3 cut(s)	26, 248, 311
MspR9I	CCNGG	2 cut(s)	167, 312
MunI	CAATTG	2 cut(s)	330, 1052
MvaI	CCWGG	1 cut(s)	167
MvnI	CGCG	1 cut(s)	34
MwoI	GCNNNNNNNGC	1 cut(s)	244
NciI	CCSGG	1 cut(s)	312
NcoI	CCATGG	1 cut(s)	783
NdeII	GATC	4 cut(s)	20, 114, 316, 826
NlaIII	CATG	6 cut(s)	787, 805, 970, 1007, 1159, 1166
NlaIV	GGNNCC	1 cut(s)	754
NmeAIII	GCCGAG	1 cut(s)	928
NsbI	TGCGCA	1 cut(s)	872
NspI	RCATGY	1 cut(s)	970
PaeI	GCATGC	1 cut(s)	970
PagI	TCATGA	2 cut(s)	1155, 1162
PasI	CCCWGGG	1 cut(s)	166
PfeI	GAWTC	6 cut(s)	46, 396, 446, 656, 1152, 1159
PkrI	GCNGC	4 cut(s)	36, 924, 997, 1000
PleI	GAGTC	4 cut(s)	249, 364, 813, 1160
PpsI	GAGTC	4 cut(s)	249, 364, 813, 1160
PshAI	GACNNNNGTC	1 cut(s)	804
PsiI	TTATAA	1 cut(s)	741
Psp6I	CCWGG	1 cut(s)	165
PspGI	CCWGG	1 cut(s)	165
PspN4I	GGNNCC	1 cut(s)	754
PspPI	GGNCC	2 cut(s)	163, 937
PstI	CTGCAG	1 cut(s)	924
PsuI	RGATCY	2 cut(s)	20, 316
RsaI	GTAC	4 cut(s)	266, 283, 583, 632
RsaNI	GTAC	4 cut(s)	265, 282, 582, 631
RseI	CAYNNNNRTG	1 cut(s)	570
SaqAI	TTAA	3 cut(s)	468, 1065, 1082
SatI	GCNGC	4 cut(s)	35, 923, 996, 999
Sau3AI	GATC	4 cut(s)	20, 114, 316, 826
Sau96I	GGNCC	2 cut(s)	163, 937
ScaI	AGTACT	2 cut(s)	266, 632
SchI	GAGTC	4 cut(s)	250, 365, 814, 1160
ScrFI	CCNGG	2 cut(s)	167, 312
SfaNI	GCATC	3 cut(s)	447, 525, 882
SfcI	CTRYAG	2 cut(s)	606, 920
SmiMI	CAYNNNNRTG	1 cut(s)	570
SmlI	CTYRAG	1 cut(s)	1134
SmoI	CTYRAG	1 cut(s)	1134
SphI	GCATGC	1 cut(s)	970
SsiI	CCGC	6 cut(s)	34, 91, 106, 238, 245, 1011
SspMI	CTAG	2 cut(s)	279, 401
StyD4I	CCNGG	2 cut(s)	165, 310
StyI	CCWWGG	1 cut(s)	783
TaiI	ACGT	2 cut(s)	67, 1131
TaqI	TCGA	2 cut(s)	483, 1059
TatI	WGTACW	2 cut(s)	264, 630
TauI	GCSGC	1 cut(s)	37
TfiI	GAWTC	6 cut(s)	46, 396, 446, 656, 1152, 1159
Tru1I	TTAA	3 cut(s)	468, 1065, 1082
Tru9I	TTAA	3 cut(s)	468, 1065, 1082
TscAI	CASTG	1 cut(s)	1030
TseI	GCWGC	3 cut(s)	922, 995, 998
TspDTI	ATGAA	3 cut(s)	162, 720, 1151
TspRI	CASTG	1 cut(s)	1030
XapI	RAATTY	3 cut(s)	674, 683, 1078
XceI	RCATGY	1 cut(s)	970
XspI	CTAG	2 cut(s)	279, 401
ZrmI	AGTACT	2 cut(s)	266, 632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.