Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007151

Physical Location & Seq

Genomic Coordinates Reverse (-)

9369190 .. 9372155

Gene Structure 2966 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1129400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGTCCCGCTTACGATCCCTAATTCGATCCACACCGTCCATCCACAAACGCATCCCAGTCCGCCAACTGTCCGCCTCACCACAACCGTCGTCGCCTCTCTCTCTGATCGCCGCGTCGAACTTCGAGTCAAACCCTCCCCAAAACCCCTTCCATGGCTCGTTCCTCAAATGGATCTCGGGCTTCGCGGTGGGCTCGGGAGTAGGTCTTCTGTACTGGTCTTCCCCCGATTCCGATTTATTCAAAACGTCATCGTTCGCTGACTGGTCAACGGCGGAGTCGGGACTGGCCGTGGAGGATCGGCCGTCACGGTCCTTTTTACGGAAGTTGTCTTTGCCCGAAATTGGCCCTGGGTTTCTCTTCGGAGATGCTTATAGGAGAAAGATTTTCTTCAACTACGAGAAGCGCATAAGGTTGCGGAGTCCGCCGGAGAAGGTTTTTGAGTACTTTGCATCCTCTAGTACTGCAGATGGAGAGTTACTTATGACACCGGGAGATCTTATGAGGGCAATTGTGCCCGTCTTCCCTCCATCTGAGTCCAACCTTGTTAGAGATGGATATTTGAGAGGGGAAAGGAATCCTGGTGATTTGCGCTGTTCTCCTTCTGAATTTTTTATGCTTTTTGATGTAAACAATGATGGGCATATTTCTTTTAAAGAGTATATTTTCTTTGTAACACTACTCAGCATCCCAGAATCAAGTTTCTGTATCGCTTTTAAAATGTTTGACCTCGATGATAGTGGACAAATTGATAAGGAAGAGTTCAAGAAAGTGATGGGTTTGATGCGAGCCTGCAATAGACAGGGCGCTTACCACAGGGCGGGACTTCGGCCTGGGCTAAAAGTCCATGGTTCCGTAGAAGATGGAGGGCTTGTGGAGTACTTTTTTGGCAAAGATGGGAAGGATTCCCTCCAACTTGATAAATTTGTCAAATTTTTGAGAGATTTACAGGATGAAATGGTGAGGTTGGAGTTTTCCCACTATGACTATAAACTACGAGGAACCATATTAGCAAGGGACTTTGCTTTGTCCATGGTTTCTTCTGCCGATATGAGTCACCTAAGCAATTTGCTTGATCGGGTTGAGGAATTGAGCAATAAACCACATCTTAGAGACGTGCGCATCACATTTGAGGAATTCAAAAGTTTTGCCGAGCTCCGTAAAAAATTGCAGCCTTTTTCTTTGGCTCTTTTCAGCTTTGGCAAAGTCAATGGGATGCTAACGCGAGAGGATTTCCAACGTGCTGCTTCTCATGTTTGTGGCGTATCTCTCACTGATAACGTGGTGGAAATCGTCTTCCAATTGTTTGATTTCAACCAAGATGGAAATTTAAGCTTGGATGAGTTCGTTAGAGTTTTACAGCAGCGAGGAAGGGACATTGCTCAACATGTGGAGACGGGAATCATGATTCATGACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005758 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006839 GO:0006851 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0009987 GO:0016020 GO:0016021 GO:0019725 GO:0019866 GO:0030001 GO:0030003 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0032592 GO:0032991 GO:0034220 GO:0034702 GO:0034703 GO:0034704 GO:0036444 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046872 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051560 GO:0051561 GO:0051562 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060401 GO:0065007 GO:0065008 GO:0070013 GO:0070509 GO:0070588 GO:0070838 GO:0072503 GO:0072507 GO:0072511 GO:0098573 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098798 GO:0098800 GO:1902495 GO:1990246 GO:1990351 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

473

Amino Acids

53.51

Weight (kDa)

6.44

Isoelectric Point (pI)

49.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_5	PF13202	237 - 258	2.3e-06	EF hand
EF-hand_7	PF13499	403 - 452	1.6e-07	EF-hand domain pair
EF-hand_8	PF13833	404 - 454	1.6e-09	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32060 AT4G32060
fragaria_vesca	FvH4_2g28590 FvH4_2g28590
malus_domestica	MD08G1152800.v1.1 MD15G1129400.v1.1
prunus_persica	Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1 Prupe.1G485000_v2.0.a1
pyrus_communis	pycom08g13170 pycom15g11660
rosa_chinensis	RchiOBHm_Chr6g0297361
rosa_laevigata	RLG00000011608
rosa_multiflora	Rmu_ssc0000109.1_g000001
rosa_roxburghii	Rroxscaffold_7G00170610
rosa_rugosa	Rorug06G0270900 Rorug06G0271000
rosa_samantha	Rh6AG382400 Rh6BG390500 Rh6CG395600 Rh6DG382600
rosa_wichuraiana	Rw6G033310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1118
AccII	CGCG	3 cut(s)	113, 185, 1222
AciI	CCGC	9 cut(s)	7, 61, 72, 111, 185, 272, 415, 422, 818
AclWI	GGATC	4 cut(s)	9, 21, 179, 303
AcoI	YGGCCR	2 cut(s)	285, 299
AcsI	RAATTY	5 cut(s)	605, 920, 929, 1133, 1324
AfaI	GTAC	4 cut(s)	212, 443, 460, 878
AfiI	CCNNNNNNNGG	4 cut(s)	152, 318, 341, 817
AflIII	ACRYGT	1 cut(s)	1384
AgsI	TTSAA	5 cut(s)	241, 391, 763, 1138, 1312
AjiI	CACGTC	1 cut(s)	1114
AjnI	CCWGG	3 cut(s)	346, 577, 829
AluBI	AGCT	3 cut(s)	1153, 1194, 1332
AluI	AGCT	3 cut(s)	1153, 1194, 1332
Alw21I	GWGCWC	1 cut(s)	1155
Alw26I	GTCTC	2 cut(s)	1104, 1385
AlwI	GGATC	4 cut(s)	9, 21, 179, 303
Ama87I	CYCGRG	2 cut(s)	175, 193
AoxI	GGCC	4 cut(s)	285, 299, 343, 827
ApeKI	GCWGC	3 cut(s)	1168, 1241, 1360
ApoI	RAATTY	5 cut(s)	605, 920, 929, 1133, 1324
ArsI	GACNNNNNNTTYG	2 cut(s)	110, 142
AspLEI	GCGC	4 cut(s)	405, 591, 806, 1119
AspS9I	GGNCC	2 cut(s)	309, 344
AsuC2I	CCSGG	1 cut(s)	489
AsuHPI	GGTGA	4 cut(s)	69, 593, 970, 1046
AvaI	CYCGRG	2 cut(s)	175, 193
AvaII	GGWCC	1 cut(s)	309
BaeGI	GKGCMC	1 cut(s)	516
BanII	GRGCYC	2 cut(s)	194, 1155
BbsI	GAAGAC	4 cut(s)	197, 210, 511, 1285
Bbv12I	GWGCWC	1 cut(s)	1155
BbvI	GCAGC	3 cut(s)	1180, 1228, 1372
BccI	CCATC	9 cut(s)	47, 461, 535, 545, 629, 766, 854, 887, 1313
BceAI	ACGGC	3 cut(s)	272, 285, 286
BciT130I	CCWGG	3 cut(s)	348, 579, 831
BcnI	CCSGG	1 cut(s)	489
BcoDI	GTCTC	2 cut(s)	1104, 1385
BfaI	CTAG	1 cut(s)	456
BfmI	CTRYAG	1 cut(s)	462
BfoI	RGCGCY	1 cut(s)	807
BglII	AGATCT	1 cut(s)	493
BisI	GCNGC	4 cut(s)	111, 1169, 1242, 1361
BlsI	GCNGC	4 cut(s)	112, 1170, 1243, 1362
BmcAI	AGTACT	3 cut(s)	443, 460, 878
Bme1390I	CCNGG	4 cut(s)	348, 489, 579, 831
Bme18I	GGWCC	1 cut(s)	309
BmeT110I	CYCGRG	2 cut(s)	175, 193
BmgBI	CACGTC	1 cut(s)	1114
BmgT120I	GGNCC	2 cut(s)	309, 344
BmiI	GGNNCC	2 cut(s)	850, 1000
BmrFI	CCNGG	4 cut(s)	348, 489, 579, 831
BmrI	ACTGGG	1 cut(s)	50
BmsI	GCATC	7 cut(s)	60, 355, 458, 693, 771, 1128, 1203
BmuI	ACTGGG	1 cut(s)	50
BpiI	GAAGAC	4 cut(s)	197, 210, 511, 1285
Bpu10I	CCTNAGC	1 cut(s)	1058
BpuMI	CCSGG	1 cut(s)	489
BsaJI	CCNNGG	7 cut(s)	151, 288, 346, 347, 830, 844, 1029
BsaXI	ACNNNNNCTCC	2 cut(s)	855, 885
Bsc4I	CCNNNNNNNGG	4 cut(s)	152, 318, 341, 817
Bse1I	ACTGG	4 cut(s)	56, 218, 266, 288
Bse3DI	GCAATG	1 cut(s)	1374
BseBI	CCWGG	3 cut(s)	348, 579, 831
BseDI	CCNNGG	7 cut(s)	151, 288, 346, 347, 830, 844, 1029
BseGI	GGATG	7 cut(s)	39, 51, 449, 684, 955, 1218, 1342
BseLI	CCNNNNNNNGG	4 cut(s)	152, 318, 341, 817
BseMI	GCAATG	1 cut(s)	1374
BseMII	CTCAG	2 cut(s)	522, 694
BseNI	ACTGG	4 cut(s)	56, 218, 266, 288
BseSI	GKGCMC	1 cut(s)	516
BseX3I	CGGCCG	1 cut(s)	299
BseXI	GCAGC	3 cut(s)	1180, 1228, 1372
Bsh1236I	CGCG	3 cut(s)	113, 185, 1222
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	4 cut(s)	287, 301, 345, 829
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	1 cut(s)	1155
BsiHKCI	CYCGRG	2 cut(s)	175, 193
BsiSI	CCGG	2 cut(s)	425, 488
BslFI	GGGAC	4 cut(s)	294, 834, 1028, 1385
BslI	CCNNNNNNNGG	4 cut(s)	152, 318, 341, 817
BsmAI	GTCTC	2 cut(s)	1104, 1385
BsmBI	CGTCTC	2 cut(s)	1104, 1385
BsmFI	GGGAC	4 cut(s)	294, 834, 1028, 1385
BsnI	GGCC	4 cut(s)	287, 301, 345, 829
BsoBI	CYCGRG	2 cut(s)	175, 193
Bsp1286I	GDGCHC	3 cut(s)	194, 516, 1155
Bsp143I	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
Bsp19I	CCATGG	3 cut(s)	151, 844, 1029
BspACI	CCGC	9 cut(s)	7, 61, 72, 111, 185, 272, 415, 422, 818
BspANI	GGCC	4 cut(s)	287, 301, 345, 829
BspCNI	CTCAG	2 cut(s)	523, 693
BspFNI	CGCG	3 cut(s)	113, 185, 1222
BspHI	TCATGA	2 cut(s)	1401, 1408
BspLI	GGNNCC	2 cut(s)	850, 1000
BspMAI	CTGCAG	1 cut(s)	466
BspPI	GGATC	4 cut(s)	9, 21, 179, 303
BsrDI	GCAATG	1 cut(s)	1374
BsrI	ACTGG	4 cut(s)	56, 218, 266, 288
BssECI	CCNNGG	7 cut(s)	151, 288, 346, 347, 830, 844, 1029
BssMI	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
BssT1I	CCWWGG	3 cut(s)	151, 844, 1029
Bst2UI	CCWGG	3 cut(s)	348, 579, 831
Bst4CI	ACNGT	4 cut(s)	36, 69, 87, 309
Bst6I	CTCTTC	2 cut(s)	362, 750
BstC8I	GCNNGC	2 cut(s)	786, 790
BstDEI	CTNAG	4 cut(s)	531, 680, 1058, 1106
BstDSI	CCRYGG	4 cut(s)	151, 288, 844, 1029
BstF5I	GGATG	7 cut(s)	39, 51, 449, 684, 955, 1218, 1342
BstFNI	CGCG	3 cut(s)	113, 185, 1222
BstH2I	RGCGCY	1 cut(s)	807
BstHHI	GCGC	4 cut(s)	405, 591, 806, 1119
BstKTI	GATC	7 cut(s)	17, 29, 108, 174, 298, 496, 1075
BstMAI	GTCTC	2 cut(s)	1104, 1385
BstMBI	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
BstMCI	CGRYCG	1 cut(s)	302
BstMWI	GCNNNNNNNGC	1 cut(s)	421
BstNI	CCWGG	3 cut(s)	348, 579, 831
BstNSI	RCATGY	1 cut(s)	1388
BstSCI	CCNGG	4 cut(s)	346, 487, 577, 829
BstSFI	CTRYAG	1 cut(s)	462
BstSLI	GKGCMC	1 cut(s)	516
BstUI	CGCG	3 cut(s)	113, 185, 1222
BstV1I	GCAGC	3 cut(s)	1180, 1228, 1372
BstV2I	GAAGAC	4 cut(s)	197, 210, 511, 1285
BstX2I	RGATCY	2 cut(s)	171, 493
BstYI	RGATCY	2 cut(s)	171, 493
BstZI	CGGCCG	1 cut(s)	299
BsuRI	GGCC	4 cut(s)	287, 301, 345, 829
BtgI	CCRYGG	4 cut(s)	151, 288, 844, 1029
BtrI	CACGTC	1 cut(s)	1114
BtsCI	GGATG	7 cut(s)	39, 51, 449, 684, 955, 1218, 1342
BtsIMutI	CAGTG	1 cut(s)	1269
Cac8I	GCNNGC	2 cut(s)	786, 790
CciI	TCATGA	2 cut(s)	1401, 1408
CfoI	GCGC	4 cut(s)	405, 591, 806, 1119
Cfr13I	GGNCC	2 cut(s)	309, 344
CseI	GACGC	1 cut(s)	102
Csp6I	GTAC	4 cut(s)	211, 442, 459, 877
CviAII	CATG	7 cut(s)	152, 845, 1030, 1250, 1385, 1402, 1409
CviQI	GTAC	4 cut(s)	211, 442, 459, 877
DdeI	CTNAG	4 cut(s)	531, 680, 1058, 1106
DpnI	GATC	7 cut(s)	16, 28, 107, 173, 297, 495, 1074
DpnII	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
DraI	TTTAAA	2 cut(s)	652, 715
EaeI	YGGCCR	2 cut(s)	285, 299
EagI	CGGCCG	1 cut(s)	299
Eam1104I	CTCTTC	2 cut(s)	362, 750
EarI	CTCTTC	2 cut(s)	362, 750
EciI	GGCGGA	4 cut(s)	50, 61, 287, 411
Ecl136II	GAGCTC	1 cut(s)	1153
EclXI	CGGCCG	1 cut(s)	299
Eco130I	CCWWGG	3 cut(s)	151, 844, 1029
Eco24I	GRGCYC	2 cut(s)	194, 1155
Eco47I	GGWCC	1 cut(s)	309
Eco52I	CGGCCG	1 cut(s)	299
Eco53kI	GAGCTC	1 cut(s)	1153
Eco88I	CYCGRG	2 cut(s)	175, 193
EcoICRI	GAGCTC	1 cut(s)	1153
EcoRI	GAATTC	1 cut(s)	1133
EcoRII	CCWGG	3 cut(s)	346, 577, 829
EcoT14I	CCWWGG	3 cut(s)	151, 844, 1029
EcoT38I	GRGCYC	2 cut(s)	194, 1155
ErhI	CCWWGG	3 cut(s)	151, 844, 1029
Esp3I	CGTCTC	2 cut(s)	1104, 1385
FaeI	CATG	7 cut(s)	155, 848, 1033, 1253, 1388, 1405, 1412
FalI	AAGNNNNNCTT	2 cut(s)	371, 403
FaqI	GGGAC	4 cut(s)	294, 834, 1028, 1385
FatI	CATG	7 cut(s)	151, 844, 1029, 1249, 1384, 1401, 1408
FauI	CCCGC	2 cut(s)	14, 811
Fnu4HI	GCNGC	4 cut(s)	111, 1169, 1242, 1361
FokI	GGATG	7 cut(s)	26, 38, 436, 671, 962, 1225, 1349
FriOI	GRGCYC	2 cut(s)	194, 1155
Fsp4HI	GCNGC	4 cut(s)	111, 1169, 1242, 1361
FspAI	RTGCGCAY	1 cut(s)	1118
FspBI	CTAG	1 cut(s)	456
FspI	TGCGCA	1 cut(s)	1118
GlaI	GCGC	4 cut(s)	404, 590, 805, 1118
GluI	GCNGC	4 cut(s)	111, 1169, 1242, 1361
HaeII	RGCGCY	1 cut(s)	807
HaeIII	GGCC	4 cut(s)	287, 301, 345, 829
HapII	CCGG	2 cut(s)	425, 488
HgaI	GACGC	1 cut(s)	102
HhaI	GCGC	4 cut(s)	405, 591, 806, 1119
Hin1II	CATG	7 cut(s)	155, 848, 1033, 1253, 1388, 1405, 1412
Hin6I	GCGC	4 cut(s)	403, 589, 804, 1117
HinP1I	GCGC	4 cut(s)	403, 589, 804, 1117
HincII	GTYRAC	1 cut(s)	267
HindII	GTYRAC	1 cut(s)	267
HindIII	AAGCTT	1 cut(s)	1330
HpaII	CCGG	2 cut(s)	425, 488
HphI	GGTGA	4 cut(s)	69, 593, 970, 1046
Hpy166II	GTNNAC	3 cut(s)	267, 628, 740
Hpy188I	TCNGA	5 cut(s)	105, 232, 362, 532, 604
Hpy188III	TCNNGA	5 cut(s)	195, 279, 763, 1402, 1409
Hpy8I	GTNNAC	3 cut(s)	267, 628, 740
Hpy99I	CGWCG	3 cut(s)	91, 94, 118
HpyAV	CCTTC	5 cut(s)	157, 424, 609, 892, 1362
HpyCH4III	ACNGT	4 cut(s)	36, 69, 87, 309
HpyCH4IV	ACGT	4 cut(s)	245, 1113, 1237, 1278
HpyCH4V	TGCA	4 cut(s)	449, 464, 792, 1168
HpyF10VI	GCNNNNNNNGC	1 cut(s)	421
HpyF3I	CTNAG	4 cut(s)	531, 680, 1058, 1106
HpySE526I	ACGT	4 cut(s)	245, 1113, 1237, 1278
Hsp92II	CATG	7 cut(s)	155, 848, 1033, 1253, 1388, 1405, 1412
HspAI	GCGC	4 cut(s)	403, 589, 804, 1117
Kzo9I	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
LmnI	GCTCC	1 cut(s)	1158
Lsp1109I	GCAGC	3 cut(s)	1180, 1228, 1372
LweI	GCATC	7 cut(s)	60, 355, 458, 693, 771, 1128, 1203
MaeI	CTAG	1 cut(s)	456
MaeII	ACGT	4 cut(s)	245, 1113, 1237, 1278
MaeIII	GTNAC	4 cut(s)	303, 474, 670, 1052
MalI	GATC	7 cut(s)	16, 28, 107, 173, 297, 495, 1074
MboI	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
MboII	GAAGA	9 cut(s)	197, 210, 349, 379, 511, 767, 869, 1029, 1285
MfeI	CAATTG	2 cut(s)	507, 1298
MflI	RGATCY	2 cut(s)	171, 493
MhlI	GDGCHC	3 cut(s)	194, 516, 1155
MlyI	GAGTC	6 cut(s)	134, 284, 427, 542, 1060, 1406
MmeI	TCCRAC	4 cut(s)	561, 934, 945, 1258
MseI	TTAA	4 cut(s)	651, 714, 1328, 1417
MslI	CAYNNNNRTG	1 cut(s)	1254
MspI	CCGG	2 cut(s)	425, 488
MspR9I	CCNGG	4 cut(s)	348, 489, 579, 831
MunI	CAATTG	2 cut(s)	507, 1298
MvaI	CCWGG	3 cut(s)	348, 579, 831
MvnI	CGCG	3 cut(s)	113, 185, 1222
MwoI	GCNNNNNNNGC	1 cut(s)	421
NciI	CCSGG	1 cut(s)	489
NcoI	CCATGG	3 cut(s)	151, 844, 1029
NdeII	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
NlaIII	CATG	7 cut(s)	155, 848, 1033, 1253, 1388, 1405, 1412
NlaIV	GGNNCC	2 cut(s)	850, 1000
NmeAIII	GCCGAG	1 cut(s)	1174
NmuCI	GTSAC	2 cut(s)	303, 1052
NsbI	TGCGCA	1 cut(s)	1118
NspI	RCATGY	1 cut(s)	1388
PagI	TCATGA	2 cut(s)	1401, 1408
PasI	CCCWGGG	1 cut(s)	347
PciI	ACATGT	1 cut(s)	1384
PfeI	GAWTC	6 cut(s)	227, 574, 692, 902, 1398, 1405
PkrI	GCNGC	4 cut(s)	112, 1170, 1243, 1362
PleI	GAGTC	6 cut(s)	133, 283, 426, 541, 1059, 1406
PpsI	GAGTC	6 cut(s)	133, 283, 426, 541, 1059, 1406
PscI	ACATGT	1 cut(s)	1384
Psp124BI	GAGCTC	1 cut(s)	1155
Psp6I	CCWGG	3 cut(s)	346, 577, 829
PspGI	CCWGG	3 cut(s)	346, 577, 829
PspN4I	GGNNCC	2 cut(s)	850, 1000
PspPI	GGNCC	2 cut(s)	309, 344
PstI	CTGCAG	1 cut(s)	466
PsuI	RGATCY	2 cut(s)	171, 493
RsaI	GTAC	4 cut(s)	212, 443, 460, 878
RsaNI	GTAC	4 cut(s)	211, 442, 459, 877
RseI	CAYNNNNRTG	1 cut(s)	1254
SacI	GAGCTC	1 cut(s)	1155
SaqAI	TTAA	4 cut(s)	651, 714, 1328, 1417
SatI	GCNGC	4 cut(s)	111, 1169, 1242, 1361
Sau3AI	GATC	7 cut(s)	14, 26, 105, 171, 295, 493, 1072
Sau96I	GGNCC	2 cut(s)	309, 344
ScaI	AGTACT	3 cut(s)	443, 460, 878
SchI	GAGTC	6 cut(s)	134, 284, 427, 542, 1060, 1406
ScrFI	CCNGG	4 cut(s)	348, 489, 579, 831
SduI	GDGCHC	3 cut(s)	194, 516, 1155
SfaNI	GCATC	7 cut(s)	60, 355, 458, 693, 771, 1128, 1203
SfcI	CTRYAG	1 cut(s)	462
SinI	GGWCC	1 cut(s)	309
SmiMI	CAYNNNNRTG	1 cut(s)	1254
SsiI	CCGC	9 cut(s)	7, 61, 72, 111, 185, 272, 415, 422, 818
SspMI	CTAG	1 cut(s)	456
SstI	GAGCTC	1 cut(s)	1155
StyD4I	CCNGG	4 cut(s)	346, 487, 577, 829
StyI	CCWWGG	3 cut(s)	151, 844, 1029
TaaI	ACNGT	4 cut(s)	36, 69, 87, 309
TaiI	ACGT	4 cut(s)	248, 1116, 1240, 1281
TaqI	TCGA	4 cut(s)	25, 116, 123, 729
TatI	WGTACW	4 cut(s)	210, 441, 458, 876
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	6 cut(s)	227, 574, 692, 902, 1398, 1405
Tru1I	TTAA	4 cut(s)	651, 714, 1328, 1417
Tru9I	TTAA	4 cut(s)	651, 714, 1328, 1417
TscAI	CASTG	1 cut(s)	1276
TseFI	GTSAC	2 cut(s)	303, 1052
TseI	GCWGC	3 cut(s)	1168, 1241, 1360
Tsp45I	GTSAC	2 cut(s)	303, 1052
TspDTI	ATGAA	2 cut(s)	966, 1397
TspGWI	ACGGA	3 cut(s)	334, 841, 1145
TspRI	CASTG	1 cut(s)	1276
VpaK11BI	GGWCC	1 cut(s)	309
XapI	RAATTY	5 cut(s)	605, 920, 929, 1133, 1324
XceI	RCATGY	1 cut(s)	1388
XspI	CTAG	1 cut(s)	456
ZrmI	AGTACT	3 cut(s)	443, 460, 878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.