MD08G1168300.v1.1

Penicillin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0018901
Physical Location & Seq
Forward (+)
20006746 .. 20013303
6558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1168300.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAATACGACCTTGAATCCTGCTCATCGTCTTCTCCTCTCACCCAAACCATTCCAATTCCTGAAAACCAATCACCCAAATCCACAAGCCAACACAAACCCACCTCCATTTCCCAGTACTCCACTCCCCCACCTCACTCTTCGACCTCCAACTCACTCAAACTCACTGTTCGGCCGAGTGAACTCGCTCCTCCCGCGTTCCCACAGCTCCTCTTCGAGCCCCTCACAACCCCAATTCGTTCTCGAAATTCTTGTCTTATTTGCCTTCTCTCTGGCCCTTCTCTGCCTGCGCCTCTTCTCCGCCGTCCTATTGCCGGAGTTTCCTCGCCGGTGGCAGGGCCTCGTGTCGTTTGCTCCCGAAGCTGAAGCCAGAACCAGTGCCTACCCTCCGCACGTGTGGCAAGCCGTTGTTGCTTACGAGGACAGGAGGTTCTTTAGGCATTTTGGGATCGACCCAGTTGGGATTGCTCGTGCAGTGGTGTCATTTTCGGCCTTAGGCGGCGGCAGCACGATTACCCAGCAGCTGGTGAAAAATACTTTCCTTAAAAATGAACGCACATTCTCAAGAAAATTGGTTGAGATGGTGTTGTCACTAGCATTGGAGAGGACAATGTCAAAGGGGGAAATATTGAGCTGCTATACGAGTAAGATATACTGGGGACATGGAATTTATGGCGTTGAATCAGCATCTGCATTTTATTTTGGTAAGCATCCATCTTGTCTAAGTTTGGGGGAGTCCTCAATGCTCGCTGGATTAATACCTGCCCCAGAACTTCGATCGCCACTCAGAGATCCTTCCAGAGGAAAAACTTTCCAAGCCAGAGTTTTGAAGAGGATGGTGGACGTAGGCTATCTTGACATTGAAACAGCACTTTCGGTCATGAAACAATATCTTCCTCTGCAAGTTAATGAGTCAGAAGCCCCTATTGTCCTTCCCCCGGGAGGAGGTAGAAACTCCAAATTCAAGGACATATGGGACTGGGAAACAGAAAGTAAAATATTGGAAGCATGTGAGGATATGGACAGATGGGCTATCAATGTCCTACATACTACTTCACCAAATTCTTTGGTGCATCCAATTACTGGTTGTGACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008144 GO:0008658 GO:0031406 GO:0033218 GO:0033293 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0097159 GO:1901363 GO:1901681
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transgly Transpeptidase

Protein Analysis

366

Amino Acids

40.62

Weight (kDa)

9.19

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transgly PF00912 124 - 279 2.1e-52 Transglycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018901)

Species Orthologous Gene IDs
malus_domestica MD08G1168300.v1.1
pyrus_communis pycom08g14380
rosa_multiflora Rmu_sc0007232.1_g000006
rosa_roxburghii Rroxscaffold_171G00441450 Rroxscaffold_7G00172600 Rroxscaffold_7G00172620
rosa_samantha Rh6AG362900 Rh6CG377400 Rh6CG377600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 769
AccB7I CCANNNNNTGG 2 cut(s) 523, 1082
AccII CGCG 1 cut(s) 196
AciI CCGC 5 cut(s) 194, 300, 389, 498, 501
AclWI GGATC 2 cut(s) 455, 785
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 4 cut(s) 246, 666, 959, 1060
AcuI CTGAAG 1 cut(s) 384
AcvI CACGTG 1 cut(s) 394
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 8 cut(s) 313, 334, 523, 800, 937, 941, 944, 1082
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 5 cut(s) 16, 680, 829, 863, 964
AloI GAACNNNNNNTCC 2 cut(s) 413, 445
AluBI AGCT 4 cut(s) 207, 362, 523, 633
AluI AGCT 4 cut(s) 207, 362, 523, 633
AlwI GGATC 2 cut(s) 455, 785
AlwNI CAGNNNCTG 2 cut(s) 523, 689
Ama87I CYCGRG 1 cut(s) 937
AoxI GGCC 4 cut(s) 172, 273, 337, 489
ApeKI GCWGC 3 cut(s) 504, 520, 633
ApoI RAATTY 4 cut(s) 246, 666, 959, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 755
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 274, 337
AsuC2I CCSGG 2 cut(s) 938, 939
AsuHPI GGTGA 4 cut(s) 33, 65, 538, 1047
AvaI CYCGRG 1 cut(s) 937
AxyI CCTNAGG 1 cut(s) 493
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 2 cut(s) 341, 468
BbrPI CACGTG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 3 cut(s) 516, 532, 620
BccI CCATC 4 cut(s) 574, 721, 829, 1020
BceAI ACGGC 2 cut(s) 287, 389
BcnI CCSGG 2 cut(s) 938, 939
BfaI CTAG 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 769
BisI GCNGC 5 cut(s) 499, 502, 505, 521, 634
BlsI GCNGC 5 cut(s) 500, 503, 506, 522, 635
BmcAI AGTACT 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 938, 939
BmeT110I CYCGRG 1 cut(s) 937
BmgT120I GGNCC 2 cut(s) 274, 337
BmrFI CCNGG 2 cut(s) 938, 939
BmrI ACTGGG 4 cut(s) 108, 449, 664, 988
BmsI GCATC 3 cut(s) 695, 718, 1081
BmuI ACTGGG 4 cut(s) 108, 449, 664, 988
BpiI GAAGAC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 547
BpuMI CCSGG 2 cut(s) 938, 939
BsaAI YACGTR 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 936, 937
Bsc4I CCNNNNNNNGG 8 cut(s) 313, 334, 523, 800, 937, 941, 944, 1082
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 6 cut(s) 114, 375, 455, 659, 983, 1087
Bse21I CCTNAGG 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 936, 937
BseGI GGATG 3 cut(s) 709, 840, 1072
BseLI CCNNNNNNNGG 8 cut(s) 313, 334, 523, 800, 937, 941, 944, 1082
BseMII CTCAG 1 cut(s) 799
BseNI ACTGG 6 cut(s) 114, 375, 455, 659, 983, 1087
BseRI GAGGAG 4 cut(s) 26, 179, 199, 957
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 516, 532, 620
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 196
Bsh1285I CGRYCG 2 cut(s) 175, 779
BshFI GGCC 4 cut(s) 174, 275, 339, 491
BsiEI CGRYCG 2 cut(s) 175, 779
BsiHKCI CYCGRG 1 cut(s) 937
BsiSI CCGG 3 cut(s) 314, 328, 938
BslFI GGGAC 2 cut(s) 672, 989
BslI CCNNNNNNNGG 8 cut(s) 313, 334, 523, 800, 937, 941, 944, 1082
BsmFI GGGAC 2 cut(s) 672, 989
BsnI GGCC 4 cut(s) 174, 275, 339, 491
BsoBI CYCGRG 1 cut(s) 937
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 3 cut(s) 447, 776, 790
BspACI CCGC 5 cut(s) 194, 300, 389, 498, 501
BspANI GGCC 4 cut(s) 174, 275, 339, 491
BspCNI CTCAG 1 cut(s) 798
BspFNI CGCG 1 cut(s) 196
BspHI TCATGA 1 cut(s) 879
BspMI ACCTGC 1 cut(s) 769
BspPI GGATC 2 cut(s) 455, 785
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 6 cut(s) 114, 375, 455, 659, 983, 1087
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 936, 937
BssMI GATC 3 cut(s) 447, 776, 790
BssSI CACGAG 2 cut(s) 341, 468
Bst2BI CACGAG 2 cut(s) 341, 468
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 4 cut(s) 144, 217, 299, 824
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 287, 402, 747
BstDEI CTNAG 3 cut(s) 493, 722, 785
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 3 cut(s) 709, 840, 1072
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 3 cut(s) 450, 779, 793
BstMBI GATC 3 cut(s) 447, 776, 790
BstMCI CGRYCG 2 cut(s) 175, 779
BstMWI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
BstNSI RCATGY 1 cut(s) 1011
BstSCI CCNGG 2 cut(s) 936, 937
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 3 cut(s) 516, 532, 620
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BstZI CGGCCG 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 493
BsuRI GGCC 4 cut(s) 174, 275, 339, 491
BtsCI GGATG 3 cut(s) 709, 840, 1072
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 164, 382, 480
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 287, 402, 747
CaiI CAGNNNCTG 2 cut(s) 523, 689
CciI TCATGA 1 cut(s) 879
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 274, 337
Cfr9I CCCGGG 1 cut(s) 937
Csp6I GTAC 1 cut(s) 117
CviAII CATG 3 cut(s) 662, 880, 1008
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 3 cut(s) 493, 722, 785
DpnI GATC 3 cut(s) 449, 778, 792
DpnII GATC 3 cut(s) 447, 776, 790
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 4 cut(s) 144, 217, 299, 824
EarI CTCTTC 4 cut(s) 144, 217, 299, 824
EciI GGCGGA 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 384
Eco72I CACGTG 1 cut(s) 394
Eco81I CCTNAGG 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 937
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 221
FaeI CATG 3 cut(s) 665, 883, 1011
FaqI GGGAC 2 cut(s) 672, 989
FatI CATG 3 cut(s) 661, 879, 1007
FauI CCCGC 1 cut(s) 201
FauNDI CATATG 1 cut(s) 971
Fnu4HI GCNGC 5 cut(s) 499, 502, 505, 521, 634
FokI GGATG 3 cut(s) 696, 847, 1059
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 5 cut(s) 499, 502, 505, 521, 634
FspBI CTAG 1 cut(s) 593
GlaI GCGC 1 cut(s) 290
GluI GCNGC 5 cut(s) 499, 502, 505, 521, 634
GsaI CCCAGC 1 cut(s) 520
HaeIII GGCC 4 cut(s) 174, 275, 339, 491
HapII CCGG 3 cut(s) 314, 328, 938
HhaI GCGC 1 cut(s) 291
Hin1II CATG 3 cut(s) 665, 883, 1011
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 4 cut(s) 16, 680, 734, 911
HpaII CCGG 3 cut(s) 314, 328, 938
HphI GGTGA 4 cut(s) 33, 65, 538, 1047
Hpy166II GTNNAC 2 cut(s) 181, 841
Hpy188I TCNGA 2 cut(s) 788, 916
Hpy188III TCNNGA 7 cut(s) 61, 242, 356, 564, 798, 854, 880
Hpy8I GTNNAC 2 cut(s) 181, 841
HpyAV CCTTC 4 cut(s) 274, 287, 804, 941
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4IV ACGT 2 cut(s) 393, 843
HpyCH4V TGCA 4 cut(s) 473, 692, 901, 1072
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
HpyF3I CTNAG 3 cut(s) 493, 722, 785
HpySE526I ACGT 2 cut(s) 393, 843
Hsp92II CATG 3 cut(s) 665, 883, 1011
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 3 cut(s) 447, 776, 790
LmnI GCTCC 3 cut(s) 192, 212, 358
Lsp1109I GCAGC 3 cut(s) 516, 532, 620
LweI GCATC 3 cut(s) 695, 718, 1081
MaeI CTAG 1 cut(s) 593
MaeII ACGT 2 cut(s) 393, 843
MaeIII GTNAC 2 cut(s) 588, 1088
MalI GATC 3 cut(s) 449, 778, 792
MboI GATC 3 cut(s) 447, 776, 790
MboII GAAGA 6 cut(s) 23, 131, 204, 286, 841, 884
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 8 cut(s) 56, 233, 246, 569, 666, 959, 1060, 1077
MlyI GAGTC 2 cut(s) 743, 920
MmeI TCCRAC 1 cut(s) 173
MseI TTAA 3 cut(s) 543, 755, 906
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 3 cut(s) 314, 328, 938
MspR9I CCNGG 2 cut(s) 938, 939
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
NciI CCSGG 2 cut(s) 938, 939
NdeI CATATG 1 cut(s) 971
NdeII GATC 3 cut(s) 447, 776, 790
NlaIII CATG 3 cut(s) 665, 883, 1011
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 588, 1088
NspI RCATGY 1 cut(s) 1011
PagI TCATGA 1 cut(s) 879
PfeI GAWTC 2 cut(s) 16, 680
PflFI GACNNNGTC 1 cut(s) 610
PflMI CCANNNNNTGG 2 cut(s) 523, 1082
PkrI GCNGC 5 cut(s) 500, 503, 506, 522, 635
Ple19I CGATCG 1 cut(s) 779
PleI GAGTC 2 cut(s) 742, 919
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 2 cut(s) 742, 919
Ppu21I YACGTR 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 755
PspCI CACGTG 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 516
PspPI GGNCC 2 cut(s) 274, 337
PstNI CAGNNNCTG 2 cut(s) 523, 689
PsuI RGATCY 1 cut(s) 790
PsyI GACNNNGTC 1 cut(s) 610
PvuI CGATCG 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 543, 755, 906
SatI GCNGC 5 cut(s) 499, 502, 505, 521, 634
Sau3AI GATC 3 cut(s) 447, 776, 790
Sau96I GGNCC 2 cut(s) 274, 337
ScaI AGTACT 1 cut(s) 118
SchI GAGTC 2 cut(s) 743, 920
ScrFI CCNGG 2 cut(s) 938, 939
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 3 cut(s) 695, 718, 1081
SgrAI CRCCGGYG 1 cut(s) 327
SmaI CCCGGG 1 cut(s) 939
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 8 cut(s) 56, 233, 246, 569, 666, 959, 1060, 1077
SsiI CCGC 5 cut(s) 194, 300, 389, 498, 501
SspI AATATT 2 cut(s) 627, 999
SspMI CTAG 1 cut(s) 593
StyD4I CCNGG 2 cut(s) 936, 937
TaaI ACNGT 1 cut(s) 168
TaiI ACGT 2 cut(s) 396, 846
TaqI TCGA 5 cut(s) 142, 215, 243, 450, 775
TaqII GACCGA 1 cut(s) 865
TasI AATT 8 cut(s) 56, 233, 246, 569, 666, 959, 1060, 1077
TatI WGTACW 1 cut(s) 116
TauI GCSGC 2 cut(s) 501, 504
TfiI GAWTC 2 cut(s) 16, 680
Tru1I TTAA 3 cut(s) 543, 755, 906
Tru9I TTAA 3 cut(s) 543, 755, 906
TscAI CASTG 3 cut(s) 171, 382, 480
TseFI GTSAC 2 cut(s) 588, 1088
TseI GCWGC 3 cut(s) 504, 520, 633
Tsp45I GTSAC 2 cut(s) 588, 1088
TspDTI ATGAA 3 cut(s) 17, 564, 896
TspMI CCCGGG 1 cut(s) 937
TspRI CASTG 3 cut(s) 171, 382, 480
Tth111I GACNNNGTC 1 cut(s) 610
Van91I CCANNNNNTGG 2 cut(s) 523, 1082
VspI ATTAAT 1 cut(s) 755
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 4 cut(s) 246, 666, 959, 1060
XceI RCATGY 1 cut(s) 1011
XmaI CCCGGG 1 cut(s) 937
XspI CTAG 1 cut(s) 593
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.