pycom08g14380

Penicillin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0018901
Physical Location & Seq
Forward (+)
13581761 .. 13586394
4634 bp
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UTR
Exon/CDS
Intron
pycom08g14380.1

Sequence Viewer

Length: 555 bp
ATGAATACGACCTTGAATCCTGCTCATCGTCTTCTCCTCTCAACCAAACCATTCCAATTCCTGAAAACCAATCACCCAAATCCACAAGCCAAGACAAACCCACCTCCATTTCCCAGTACTCCACTCCCCCACCTCACTCTTCGACCTCCAACTCACTCAAACTCACTGTTCGGCCGAGTGAACTCGCTCCTCCCGCGTTCCCACAGCTCCTCTTCGAGCCCCTCACAACCCCAATTCGTTCTCGAAATTCTTGTCCTATTTGCCTTCTCTCTTGCCCTTCTCTGCCTGCGCCTCTTCTCCGCCGTCCTATTGCCGGAGTTTCCTCGCCGGTGGCAGGGCCTCGTGGCGTTTGCTCCCAAAGCTGAAGCCAGAACCAGTGCCTACCCTCCGCATGTGTGGCAAGCCGTTGTTGCTTACGAGGACAGGAGGTTCTTTAGGCATTTTGGGATCGACCCAGTTGGGATTGCTCGTGCAGTGGTGTCATTTTCGGCCTTAGGCGGCGGCAGCACGATTACCCAGCAGGTACAACTTTCTGGTGAAAAATACTTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008144 GO:0008658 GO:0031406 GO:0033218 GO:0033293 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0097159 GO:1901363 GO:1901681
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transgly Transpeptidase

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

10.91

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transgly PF00912 124 - 175 4.4e-14 Transglycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018901)

Species Orthologous Gene IDs
malus_domestica MD08G1168300.v1.1
pyrus_communis pycom08g14380
rosa_multiflora Rmu_sc0007232.1_g000006
rosa_roxburghii Rroxscaffold_171G00441450 Rroxscaffold_7G00172600 Rroxscaffold_7G00172620
rosa_samantha Rh6AG362900 Rh6CG377400 Rh6CG377600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AccII CGCG 1 cut(s) 196
AciI CCGC 5 cut(s) 194, 300, 389, 498, 501
AclWI GGATC 1 cut(s) 455
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 2 cut(s) 118, 525
AfiI CCNNNNNNNGG 2 cut(s) 313, 334
AgsI TTSAA 1 cut(s) 16
AloI GAACNNNNNNTCC 2 cut(s) 413, 445
AluBI AGCT 2 cut(s) 207, 362
AluI AGCT 2 cut(s) 207, 362
AlwI GGATC 1 cut(s) 455
AoxI GGCC 3 cut(s) 172, 337, 489
ApeKI GCWGC 1 cut(s) 504
ApoI RAATTY 1 cut(s) 246
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 65, 548
AxyI CCTNAGG 1 cut(s) 493
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 2 cut(s) 341, 468
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 516
BceAI ACGGC 2 cut(s) 287, 389
BfuAI ACCTGC 1 cut(s) 511
BisI GCNGC 3 cut(s) 499, 502, 505
BlsI GCNGC 3 cut(s) 500, 503, 506
BmcAI AGTACT 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 337
BmrI ACTGGG 2 cut(s) 108, 449
BmuI ACTGGG 2 cut(s) 108, 449
BpiI GAAGAC 1 cut(s) 23
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 334
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 3 cut(s) 114, 375, 455
Bse21I CCTNAGG 1 cut(s) 493
BseLI CCNNNNNNNGG 2 cut(s) 313, 334
BseNI ACTGG 3 cut(s) 114, 375, 455
BseRI GAGGAG 3 cut(s) 26, 179, 199
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 1 cut(s) 516
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 196
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 3 cut(s) 174, 339, 491
BsiEI CGRYCG 1 cut(s) 175
BsiSI CCGG 2 cut(s) 314, 328
BslI CCNNNNNNNGG 2 cut(s) 313, 334
BsnI GGCC 3 cut(s) 174, 339, 491
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 1 cut(s) 447
BspACI CCGC 5 cut(s) 194, 300, 389, 498, 501
BspANI GGCC 3 cut(s) 174, 339, 491
BspFNI CGCG 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 3 cut(s) 114, 375, 455
BssAI RCCGGY 1 cut(s) 327
BssMI GATC 1 cut(s) 447
BssSI CACGAG 2 cut(s) 341, 468
Bst2BI CACGAG 2 cut(s) 341, 468
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 3 cut(s) 144, 217, 299
BstC8I GCNNGC 2 cut(s) 287, 402
BstDEI CTNAG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 1 cut(s) 450
BstMBI GATC 1 cut(s) 447
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
BstNSI RCATGY 1 cut(s) 395
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 516
BstV2I GAAGAC 1 cut(s) 23
BstZI CGGCCG 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 493
BsuRI GGCC 3 cut(s) 174, 339, 491
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 164, 382, 480
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 287, 402
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 117, 524
CviAII CATG 1 cut(s) 392
CviJI RGCY 9 cut(s) 89, 174, 207, 219, 339, 362, 368, 404, 491
CviKI_1 RGCY 9 cut(s) 89, 174, 207, 219, 339, 362, 368, 404, 491
CviQI GTAC 2 cut(s) 117, 524
DdeI CTNAG 1 cut(s) 493
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 144, 217, 299
EarI CTCTTC 3 cut(s) 144, 217, 299
EciI GGCGGA 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 384
Eco81I CCTNAGG 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 221
FaeI CATG 1 cut(s) 395
FaiI YATR 1 cut(s) 393
FatI CATG 1 cut(s) 391
FauI CCCGC 1 cut(s) 201
Fnu4HI GCNGC 3 cut(s) 499, 502, 505
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 499, 502, 505
GlaI GCGC 1 cut(s) 290
GluI GCNGC 3 cut(s) 499, 502, 505
GsaI CCCAGC 1 cut(s) 520
HaeIII GGCC 3 cut(s) 174, 339, 491
HapII CCGG 2 cut(s) 314, 328
HhaI GCGC 1 cut(s) 291
Hin1II CATG 1 cut(s) 395
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 1 cut(s) 16
HpaII CCGG 2 cut(s) 314, 328
HphI GGTGA 2 cut(s) 65, 548
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 2 cut(s) 61, 242
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 2 cut(s) 274, 287
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
HpyF3I CTNAG 1 cut(s) 493
Hsp92II CATG 1 cut(s) 395
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 1 cut(s) 447
LmnI GCTCC 3 cut(s) 192, 212, 358
Lsp1109I GCAGC 1 cut(s) 516
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 4 cut(s) 23, 131, 204, 286
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 3 cut(s) 56, 233, 246
MmeI TCCRAC 1 cut(s) 173
MseI TTAA 1 cut(s) 553
MspI CCGG 2 cut(s) 314, 328
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 5 cut(s) 193, 359, 397, 410, 504
NdeII GATC 1 cut(s) 447
NlaIII CATG 1 cut(s) 395
NmeAIII GCCGAG 1 cut(s) 200
NspI RCATGY 1 cut(s) 395
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 500, 503, 506
PspFI CCCAGC 1 cut(s) 516
PspPI GGNCC 1 cut(s) 337
RsaI GTAC 2 cut(s) 118, 525
RsaNI GTAC 2 cut(s) 117, 524
SaqAI TTAA 1 cut(s) 553
SatI GCNGC 3 cut(s) 499, 502, 505
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 1 cut(s) 337
ScaI AGTACT 1 cut(s) 118
SduI GDGCHC 1 cut(s) 221
SetI ASST 8 cut(s) 14, 106, 135, 148, 209, 364, 431, 525
SgrAI CRCCGGYG 1 cut(s) 327
Sse9I AATT 3 cut(s) 56, 233, 246
SsiI CCGC 5 cut(s) 194, 300, 389, 498, 501
TaaI ACNGT 1 cut(s) 168
TaqI TCGA 4 cut(s) 142, 215, 243, 450
TasI AATT 3 cut(s) 56, 233, 246
TatI WGTACW 1 cut(s) 116
TauI GCSGC 2 cut(s) 501, 504
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 553
Tru9I TTAA 1 cut(s) 553
TscAI CASTG 3 cut(s) 171, 382, 480
TseI GCWGC 1 cut(s) 504
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 171, 382, 480
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 395
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.