Rroxscaffold_7G00172600

Penicillin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018901
Physical Location & Seq
Forward (+)
12809009 .. 12811134
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00172600.1

Sequence Viewer

Length: 699 bp
ATGCTGCAATCATACCTGGAGATTCCAATGCTTTTTCCAAAATCAAATGGAATCGCAACAACTTATCTTATTGTTAACCGATTGCTCTTACAATGGGAAATCAACGAGATCAAATGGCTGCAGCTCATGCCTAAAACCTGTGAGGATGTTACTTCCCTAACCGATGAACCAGGGCTCCCATTTGAAACTAGCATGAACTTGATCACCACCCATCGTCTTGTAATCTCAACCAAACCATCCCAATTGATCAATACCTGTCACCCAATTTTCCAATCAAACGAAAACTCACTTCAATTCCCCAATCCCTCACTCCCTTTTCTCACATTTCAATCTCCAACTCGCTCCAACTCACTATTCCTCCGAGTCCACTCACTTTTCCCGCCTTCCCACAACTCCTCTCCAAACCCTCCAAAACCCAACACATTCCCGAACTCCTCCTCCTCTTCGCATTCTCTTCACTCCTCCTCTGCCTCCGCCTCTTCTCCGGCGTCTTACTTCCCGATTGCCCTCGCCGGTGGCAAACTCTCGTCGCTTTTGCTCCAAAAGCCCAAGCCGAAACCAGCACCTATCCCCCACACGTGTGGCGAGCCATTGTTGCTTATGAGGGCAGAAGGTTCTTTAGCCATTGTGGGATTGACCCAGTTGGGCTTGGCCGCGCAGTGGTCTCCTTTTCCGCCCCTGCTGGTGGCAGCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008144 GO:0008658 GO:0031406 GO:0033218 GO:0033293 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0097159 GO:1901363 GO:1901681
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transgly Transpeptidase

Protein Analysis

232

Amino Acids

25.33

Weight (kDa)

8.85

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018901)

Species Orthologous Gene IDs
malus_domestica MD08G1168300.v1.1
pyrus_communis pycom08g14380
rosa_multiflora Rmu_sc0007232.1_g000006
rosa_roxburghii Rroxscaffold_171G00441450 Rroxscaffold_7G00172600 Rroxscaffold_7G00172620
rosa_samantha Rh6AG362900 Rh6CG377400 Rh6CG377600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 656
AciI CCGC 4 cut(s) 380, 474, 654, 674
AcoI YGGCCR 1 cut(s) 651
AcvI CACGTG 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 488
AfiI CCNNNNNNNGG 2 cut(s) 660, 685
AflIII ACRYGT 2 cut(s) 576, 578
AgsI TTSAA 3 cut(s) 185, 293, 329
AjnI CCWGG 2 cut(s) 15, 169
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AleI CACNNNNGTG 1 cut(s) 579
AloI GAACNNNNNNTCC 4 cut(s) 159, 191, 422, 454
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw26I GTCTC 1 cut(s) 669
AoxI GGCC 1 cut(s) 651
ApeKI GCWGC 4 cut(s) 4, 118, 121, 689
AspLEI GCGC 1 cut(s) 658
AsuHPI GGTGA 2 cut(s) 196, 251
BanII GRGCYC 1 cut(s) 177
BbrPI CACGTG 1 cut(s) 579
BbvI GCAGC 2 cut(s) 105, 133
BccI CCATC 2 cut(s) 219, 244
BciT130I CCWGG 2 cut(s) 17, 171
BclI TGATCA 2 cut(s) 201, 246
BcoDI GTCTC 1 cut(s) 669
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 119
BisI GCNGC 5 cut(s) 5, 119, 122, 654, 690
BlsI GCNGC 5 cut(s) 6, 120, 123, 655, 691
Bme1390I CCNGG 2 cut(s) 17, 171
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 2 cut(s) 17, 171
BmrI ACTGGG 1 cut(s) 634
BmuI ACTGGG 1 cut(s) 634
BpmI CTGGAG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 579
BsaHI GRCGYC 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 170
BsaXI ACNNNNNCTCC 6 cut(s) 159, 189, 342, 372, 422, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 685
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 640
BseBI CCWGG 2 cut(s) 17, 171
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 2 cut(s) 151, 236
BseLI CCNNNNNNNGG 2 cut(s) 660, 685
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 6 cut(s) 385, 424, 427, 430, 451, 454
BseXI GCAGC 2 cut(s) 105, 133
Bsh1236I CGCG 1 cut(s) 656
BshFI GGCC 1 cut(s) 653
BsiSI CCGG 2 cut(s) 485, 513
BslI CCNNNNNNNGG 2 cut(s) 660, 685
BsmAI GTCTC 1 cut(s) 669
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 1 cut(s) 653
Bso31I GGTCTC 1 cut(s) 669
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 3 cut(s) 108, 201, 246
BspACI CCGC 4 cut(s) 380, 474, 654, 674
BspANI GGCC 1 cut(s) 653
BspFNI CGCG 1 cut(s) 656
BspLI GGNNCC 1 cut(s) 176
BspMAI CTGCAG 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 512
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 3 cut(s) 108, 201, 246
BssNI GRCGYC 1 cut(s) 488
Bst2UI CCWGG 2 cut(s) 17, 171
Bst6I CTCTTC 3 cut(s) 448, 459, 484
BstACI GRCGYC 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 587
BstF5I GGATG 2 cut(s) 151, 236
BstFNI CGCG 1 cut(s) 656
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 3 cut(s) 111, 204, 249
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 3 cut(s) 108, 201, 246
BstMWI GCNNNNNNNGC 3 cut(s) 127, 544, 595
BstNI CCWGG 2 cut(s) 17, 171
BstSCI CCNGG 2 cut(s) 15, 169
BstSFI CTRYAG 1 cut(s) 119
BstUI CGCG 1 cut(s) 656
BstV1I GCAGC 2 cut(s) 105, 133
BstXI CCANNNNNNTGG 1 cut(s) 581
BsuRI GGCC 1 cut(s) 653
BtsCI GGATG 2 cut(s) 151, 236
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 1 cut(s) 665
Cac8I GCNNGC 1 cut(s) 587
CfoI GCGC 1 cut(s) 658
Cfr10I RCCGGY 1 cut(s) 512
CseI GACGC 1 cut(s) 477
CviAII CATG 2 cut(s) 127, 193
CviJI RGCY 9 cut(s) 118, 124, 175, 547, 553, 589, 623, 648, 653
CviKI_1 RGCY 9 cut(s) 118, 124, 175, 547, 553, 589, 623, 648, 653
DpnI GATC 3 cut(s) 110, 203, 248
DpnII GATC 3 cut(s) 108, 201, 246
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 3 cut(s) 448, 459, 484
EarI CTCTTC 3 cut(s) 448, 459, 484
EciI GGCGGA 2 cut(s) 463, 663
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 669
Eco72I CACGTG 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 15, 169
EcoT38I GRGCYC 1 cut(s) 177
FaeI CATG 2 cut(s) 130, 196
FaiI YATR 4 cut(s) 13, 128, 194, 602
FatI CATG 2 cut(s) 126, 192
FauI CCCGC 1 cut(s) 387
FbaI TGATCA 2 cut(s) 201, 246
Fnu4HI GCNGC 5 cut(s) 5, 119, 122, 654, 690
FokI GGATG 2 cut(s) 158, 223
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 5 cut(s) 5, 119, 122, 654, 690
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 657
GluI GCNGC 5 cut(s) 5, 119, 122, 654, 690
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 1 cut(s) 653
HapII CCGG 2 cut(s) 485, 513
HgaI GACGC 1 cut(s) 477
HhaI GCGC 1 cut(s) 658
Hin1I GRCGYC 1 cut(s) 488
Hin1II CATG 2 cut(s) 130, 196
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 3 cut(s) 22, 51, 363
HpaI GTTAAC 1 cut(s) 76
HpaII CCGG 2 cut(s) 485, 513
HphI GGTGA 2 cut(s) 196, 251
Hpy166II GTNNAC 2 cut(s) 76, 367
Hpy188I TCNGA 1 cut(s) 362
Hpy188III TCNNGA 2 cut(s) 427, 499
Hpy8I GTNNAC 2 cut(s) 76, 367
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 2 cut(s) 393, 605
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 2 cut(s) 7, 121
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 544, 595
HpySE526I ACGT 1 cut(s) 578
Hsp92I GRCGYC 1 cut(s) 488
Hsp92II CATG 2 cut(s) 130, 196
HspAI GCGC 1 cut(s) 656
Ksp22I TGATCA 2 cut(s) 201, 246
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 108, 201, 246
LmnI GCTCC 3 cut(s) 180, 347, 543
Lsp1109I GCAGC 2 cut(s) 105, 133
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 2 cut(s) 148, 257
MalI GATC 3 cut(s) 110, 203, 248
MboI GATC 3 cut(s) 108, 201, 246
MboII GAAGA 3 cut(s) 435, 446, 471
MfeI CAATTG 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 3 cut(s) 242, 264, 293
MlyI GAGTC 1 cut(s) 372
MmeI TCCRAC 2 cut(s) 359, 369
MseI TTAA 1 cut(s) 75
MslI CAYNNNNRTG 1 cut(s) 579
MspI CCGG 2 cut(s) 485, 513
MspR9I CCNGG 2 cut(s) 17, 171
MunI CAATTG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 448
MvaI CCWGG 2 cut(s) 17, 171
MvnI CGCG 1 cut(s) 656
MwoI GCNNNNNNNGC 3 cut(s) 127, 544, 595
NdeII GATC 3 cut(s) 108, 201, 246
NlaIII CATG 2 cut(s) 130, 196
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 579
PctI GAATGC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 22, 51
PkrI GCNGC 5 cut(s) 6, 120, 123, 655, 691
PleI GAGTC 1 cut(s) 371
PmaCI CACGTG 1 cut(s) 579
PmlI CACGTG 1 cut(s) 579
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 15, 169
PspCI CACGTG 1 cut(s) 579
PspGI CCWGG 2 cut(s) 15, 169
PspN4I GGNNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 123
RseI CAYNNNNRTG 1 cut(s) 579
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 5 cut(s) 5, 119, 122, 654, 690
Sau3AI GATC 3 cut(s) 108, 201, 246
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 2 cut(s) 17, 171
SduI GDGCHC 1 cut(s) 177
SetI ASST 7 cut(s) 18, 126, 140, 257, 568, 581, 616
SfcI CTRYAG 1 cut(s) 119
SgrAI CRCCGGYG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 579
Sse9I AATT 3 cut(s) 242, 264, 293
SsiI CCGC 4 cut(s) 380, 474, 654, 674
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 15, 169
TaiI ACGT 1 cut(s) 581
TasI AATT 3 cut(s) 242, 264, 293
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 2 cut(s) 22, 51
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 665
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 4 cut(s) 4, 118, 121, 689
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 2 cut(s) 180, 209
TspRI CASTG 1 cut(s) 665
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.