MD08G1179700.v1.1

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002612
Physical Location & Seq
Reverse (-)
22121738 .. 22127401
5664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1179700.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGGGTTCTCGAGGAAGGTTGTTATTTGATCTCAATGAACCCCCTGCCGAGGATAATGAAGGGAGTGATGGCGCCCTCAGCTTCCAGCCACAAAAGGCACTTCCTTCTTCAAACCCCCACACTTCAGAAGTGCTTGCAGTAGCAGCCGTTGCACCAAGAATTGTTAATAATCATGCTTTTTCACATGCATCGTCTGTGTCAGGTTTTCAACCTTTTGTTCGGCCAAAAAATGCTCATGGCTTTGAAGGTGATGCTGACGAGAAATCAAGAGATAGTAATCCCAAATATACATCAGTATCTAAATCAAGTAATTGTGAGGACATGAAACCTGTACCATGTTTAGCTTCTGCTTCTGCAAATGGTCCATCTGTGGAAAGAGAAGAAGGAGAGTGGTCTGATGATGCCGAGGGATCTGCTGAAGCAGGTGGAACTGGCAGTTTGCATGAACAGGGTACAAGTTTACAAGGACAACCGGGAGGGATTGTTGAATGTGCTTCTGGTGTTGCTCCAGATATTAGTTCTTGTGATATTAAAACTTCAGAAGGTCTCAAGGATAAGAACACCAGTCATACTTCTTTAGGAGTAGATGATCAGAATAGTACTAGTAGCCGCATTTCAGACAGCAATGTTAAAGGTCAGGCAGCCATGGATGGTCAGGATGAACACGGATTGGTTTTGAAACAAGAAAAAGTTAAAGGCATTGAAGCAAGCCATGCACTCAAGTGTGCAAATAATCCAATGAAGAGGAAGCTGAGCCAACAGAATGAAGCAAAGCTGGGAAAGAAACGTAATAGACAGACAATGTTCCTTAATTTGGATGATGTTAAGCAAGCTGGCACTATCAAAAGTTCAACACCAAGAAGACAGACCTTTCCCTCACCTGTTACAACTCGCACTTTGAAGGACGTTCGTACCATTACTCTGCCTGCAGATTGTGTTGGGGAAAAGCAGTCACAATCAATGATTAAGGACCAGAAACAAGTTGATGTTTTATGTAATGATGGAGGAACTGCCGCAGAGTCTAGTGATTCCAAGTCTGAAACTAACGGGGATTTTAGTTATGGATCACTGTCTAGGACCAGAAGGCAAAATGGTGACAATGATCCTTCCACAGAGGTCTTACCACCAATTCCAAGGCAGAGTTCATGGAAGCAGCCCACGGATACAAGGCAGCTGAAGAATTCACATGTTGCTAATAGGAAGCCAGCTCTGATCACTCAAAGCTCCATGGATTCCAAATCTGGAAACAAGAAACTTCTTCCTGTCAAAAAGCAAACGGCAATCAGTAACACACACCAAGACACATCAGTTGAGCGTCTTATAAGGGAGGTTACAAATGAGAAGTTTTGGCATCACCCAGGGGAGACTGACCTCCAATGTGTTCCTGAAAAGTTTGAATCTGTTGAGGAGTATGTTAGAGTATTTGAACCGCTTCTGTTTGAAGAATGCCGTGCACAACTTTACAGCACCTGGGAGGAATTAACAGAGGGTGTTTCTAGAGATGCTCATATAACTGTTCGTGTAAGAAGCATTGAAAGGAGAGAGAGAGGGTGGTATGATGTAATAGTCCTTCCAGTTAGTGAATGCAAGTGGACATTTAAAGAGGGGGATGTTGCAGTTCTTTCAACTCCTCGGCCTGGATCAGTCAGATCCAAAAGGAACAACTCTTCAGCTGAGGGCGATGAGGAGCCTGAAATCAGTGGGCGTGTGGCTGGTACTGTCCGGCGACATATTCCTATCGATACTCGTGATCCCCCAGGGGCAATCCTTCATTTTTATGTTGGGGACTCCTATGATTCTAACAGCTTGGTTGATGATGATCATGTTCTAAGAAAGCTACAGCCCAAAGGAATTTGGTATTTAACTGTGCTTGGTTCTCTAGCAACCACCCAGCGCGAGTATATTGCACTGCATGCATTTCGTCGTCTGAATATGCAGATGCAAGCAGCAATTCTACGGCCCAGTCCTGAACACTTTCCAAAATACGAGCAGCAGTCCCCTGCTATGCCAGAATGCTTCACACCGAATTTCATTGATCATTTGCATAGGACCTTTAATGGGCCCCAGCTATCAGCAATCCAATGGGCTGCAATGCATACAGCTGCAGGTACAAGTGGTGGAAAGAGGCAAGATCCATGGCCTTTTACACTTGTTCAAGGACCTCCAGGAACAGGTAAGACACATACGGTCTGGGGAATGCTAAACGTCATCCATCTGGTTCAGTATCAGCAGTACTACACTTCTTTGCTGAAGAAACTAGCACCTGAAAGCTATAAGCAAAATAGTGAGACCAACTTTGATAATGTTGCTACGGGATCAATAGATGAGGTTCTTCAGAACATGGACCAGAATCTCCTTCGTACACTACCCAAGCTCTGTCCAAAGCCTAGAATGTTGGTTTGCGCCCCTTCTAATGCTGCAACAGATGAGCTTCTTTCCCGTGTTCTTGACCGTGGATTCATTGATGGCGAGATGAAAGTTTATCGACCTGATGTAGCCCGAGTAGGTGTGGATTCACAGACACGTGCTGCCCAGGCTGTATCCGTTGAGCGGAGAACTGAACAGCTTCTGGTTAAGAACCGTGAGGAAGTTTTAGGGTGGATGCATCAGTTGAGAAATCGTGAAGCTCAGTTGTCCGTGCAGATATCTAATCTTCAAAGAGACCTTACTGTTGCAGCTGCTGCTGTTCGTTCCCAAGGATCAGTTGGTGTTGATCCTGATGTTTTGGTGGCTCGGGACCAGAATCGAGATACTTTGCTGCAGATTCTTGCAGCTGTTGTTGAAAACAGGGATAAAACTCTAGTTGAGCTATCTCGCCTGTTTATTTTAGAAAGCAAGTTTCGTGCTGGTGGCAATTTTAACTTGGAAGAAGCTCGAGCTAATCTTGAGGCAAGTTTTGCCAATGAGGCTGAGATTGTTTTCACTACTGTTTCAAGCAGTGGTCGCAAGCTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 Cytochrom_B561

Protein Analysis

986

Amino Acids

108.73

Weight (kDa)

6.36

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_11 PF13086 690 - 983 1.4e-32 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
AarI CACCTGC 1 cut(s) 413
Acc36I ACCTGC 2 cut(s) 413, 2096
AccB1I GGYRCC 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 2551
AccII CGCG 1 cut(s) 1896
AciI CCGC 4 cut(s) 610, 1014, 1430, 2551
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 3 cut(s) 1180, 1851, 2026
AcuI CTGAAG 7 cut(s) 108, 438, 522, 1196, 1653, 2270, 2318
AcvI CACGTG 1 cut(s) 2525
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 8 cut(s) 333, 454, 601, 913, 1717, 2110, 2234, 2362
AfiI CCNNNNNNNGG 6 cut(s) 49, 814, 1637, 2058, 2171, 2550
AflIII ACRYGT 2 cut(s) 1186, 2522
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 6 cut(s) 1357, 1469, 1636, 1756, 2164, 2532
AjuI GAANNNNNNNTTGG 2 cut(s) 1126, 1158
AleI CACNNNNGTG 1 cut(s) 721
Alw21I GWGCWC 1 cut(s) 1456
Alw26I GTCTC 4 cut(s) 551, 1358, 2282, 2655
Alw44I GTGCAC 1 cut(s) 1452
AlwNI CAGNNNCTG 3 cut(s) 1470, 2569, 2681
Ama87I CYCGRG 4 cut(s) 9, 2499, 2733, 2874
AoxI GGCC 5 cut(s) 221, 1634, 1958, 2060, 2138
ApaI GGGCCC 1 cut(s) 2064
ApaLI GTGCAC 1 cut(s) 1452
ApoI RAATTY 3 cut(s) 1180, 1851, 2026
Asp700I GAANNNNTTC 3 cut(s) 1431, 1974, 2565
AspLEI GCGC 3 cut(s) 74, 1896, 2405
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 4 cut(s) 260, 870, 1106, 1346
AvaI CYCGRG 4 cut(s) 9, 2499, 2733, 2874
AvaII GGWCC 7 cut(s) 362, 970, 1077, 2049, 2159, 2344, 2737
BaeGI GKGCMC 2 cut(s) 1456, 2064
BaeI ACNNNNGTAYC 4 cut(s) 279, 312, 895, 928
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 2064
BarI GAAGNNNNNNTAC 2 cut(s) 1554, 1586
BauI CACGAG 1 cut(s) 1746
BbrPI CACGTG 1 cut(s) 2525
BbsI GAAGAC 1 cut(s) 868
Bbv12I GWGCWC 1 cut(s) 1456
BbvCI CCTCAGC 2 cut(s) 77, 1674
BccI CCATC 6 cut(s) 62, 373, 644, 995, 2220, 2459
BceAI ACGGC 4 cut(s) 131, 1293, 1434, 1973
BcgI CGANNNNNNTGC 6 cut(s) 395, 429, 1886, 1901, 1920, 1935
BciT130I CCWGG 6 cut(s) 1359, 1471, 1638, 1758, 2166, 2534
BciVI GTATCC 2 cut(s) 1156, 2551
BclI TGATCA 4 cut(s) 589, 1212, 1819, 2035
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 4 cut(s) 551, 1358, 2282, 2655
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 8 cut(s) 603, 1023, 1074, 1497, 1880, 2258, 2388, 2801
BfmI CTRYAG 4 cut(s) 927, 1838, 2103, 2759
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 2 cut(s) 413, 2096
BfuI GTATCC 2 cut(s) 1156, 2551
BglI GCCNNNNNGGC 1 cut(s) 2906
BlpI GCTNAGC 1 cut(s) 752
BmcAI AGTACT 2 cut(s) 601, 2234
Bme1390I CCNGG 7 cut(s) 474, 1359, 1471, 1638, 1758, 2166, 2534
Bme18I GGWCC 7 cut(s) 362, 970, 1077, 2049, 2159, 2344, 2737
BmeT110I CYCGRG 4 cut(s) 9, 2499, 2733, 2874
BmiI GGNNCC 5 cut(s) 73, 1689, 2062, 2063, 2738
BmrFI CCNGG 7 cut(s) 474, 1359, 1471, 1638, 1758, 2166, 2534
BmrI ACTGGG 1 cut(s) 1956
BmsI GCATC 8 cut(s) 197, 241, 391, 1360, 1492, 1929, 2592, 2614
BmuI ACTGGG 1 cut(s) 1956
BpiI GAAGAC 1 cut(s) 868
BpmI CTGGAG 3 cut(s) 492, 2148, 2936
Bpu10I CCTNAGC 2 cut(s) 77, 1674
Bpu1102I GCTNAGC 1 cut(s) 752
BpuEI CTTGAG 3 cut(s) 533, 704, 2906
BpuMI CCSGG 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 1740
BsaAI YACGTR 1 cut(s) 2525
BsaBI GATNNNNATC 2 cut(s) 276, 1818
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 3 cut(s) 551, 2282, 2655
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 814, 1637, 2058, 2171, 2550
Bse1I ACTGG 5 cut(s) 436, 564, 1574, 1962, 2953
Bse3DI GCAATG 2 cut(s) 631, 2097
Bse8I GATNNNNATC 2 cut(s) 276, 1818
BseBI CCWGG 6 cut(s) 1359, 1471, 1638, 1758, 2166, 2534
BseCI ATCGAT 1 cut(s) 1740
BseGI GGATG 6 cut(s) 655, 664, 823, 1615, 2208, 2607
BseJI GATNNNNATC 2 cut(s) 276, 1818
BseLI CCNNNNNNNGG 6 cut(s) 49, 814, 1637, 2058, 2171, 2550
BseMI GCAATG 2 cut(s) 631, 2097
BseMII CTCAG 5 cut(s) 91, 743, 1665, 2642, 2901
BseNI ACTGG 5 cut(s) 436, 564, 1574, 1962, 2953
BseRI GAGGAG 3 cut(s) 1421, 1620, 1700
BseSI GKGCMC 2 cut(s) 1456, 2064
BseYI CCCAGC 3 cut(s) 775, 1890, 2064
BsgI GTGCAG 1 cut(s) 2660
Bsh1236I CGCG 1 cut(s) 1896
BshFI GGCC 5 cut(s) 223, 1636, 1960, 2062, 2140
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 1740
BsiHKAI GWGCWC 1 cut(s) 1456
BsiHKCI CYCGRG 4 cut(s) 9, 2499, 2733, 2874
BsiSI CCGG 2 cut(s) 473, 1723
BslFI GGGAC 3 cut(s) 1799, 1981, 2750
BslI CCNNNNNNNGG 6 cut(s) 49, 814, 1637, 2058, 2171, 2550
BsmAI GTCTC 4 cut(s) 551, 1358, 2282, 2655
BsmFI GGGAC 3 cut(s) 1799, 1981, 2750
BsmI GAATGC 4 cut(s) 1451, 1589, 2018, 2202
BsnI GGCC 5 cut(s) 223, 1636, 1960, 2062, 2140
Bso31I GGTCTC 3 cut(s) 551, 2282, 2655
BsoBI CYCGRG 4 cut(s) 9, 2499, 2733, 2874
Bsp120I GGGCCC 1 cut(s) 2060
Bsp1286I GDGCHC 2 cut(s) 1456, 2064
Bsp1720I GCTNAGC 1 cut(s) 752
Bsp19I CCATGG 3 cut(s) 645, 1227, 2135
BspACI CCGC 4 cut(s) 610, 1014, 1430, 2551
BspANI GGCC 5 cut(s) 223, 1636, 1960, 2062, 2140
BspCNI CTCAG 5 cut(s) 90, 744, 1666, 2641, 2902
BspDI ATCGAT 1 cut(s) 1740
BspFNI CGCG 1 cut(s) 1896
BspLI GGNNCC 5 cut(s) 73, 1689, 2062, 2063, 2738
BspMAI CTGCAG 3 cut(s) 931, 2107, 2763
BspMI ACCTGC 2 cut(s) 413, 2096
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 3 cut(s) 551, 2282, 2655
BsrBI CCGCTC 1 cut(s) 2551
BsrDI GCAATG 2 cut(s) 631, 2097
BsrI ACTGG 5 cut(s) 436, 564, 1574, 1962, 2953
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 1746
BssT1I CCWWGG 5 cut(s) 645, 1133, 1227, 2135, 2695
Bst2BI CACGAG 1 cut(s) 1746
Bst2UI CCWGG 6 cut(s) 1359, 1471, 1638, 1758, 2166, 2534
Bst4CI ACNGT 9 cut(s) 1071, 1516, 1720, 1867, 2188, 2453, 2582, 2671, 2929
Bst6I CTCTTC 2 cut(s) 737, 1672
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 5 cut(s) 149, 713, 1913, 2681, 2897
BstBAI YACGTR 1 cut(s) 2525
BstC8I GCNNGC 9 cut(s) 135, 709, 831, 835, 927, 1206, 1914, 1944, 2948
BstDEI CTNAG 6 cut(s) 77, 752, 1674, 1829, 2628, 2910
BstDSI CCRYGG 5 cut(s) 645, 1158, 1227, 2135, 2452
BstF5I GGATG 6 cut(s) 655, 664, 823, 1615, 2208, 2607
BstFNI CGCG 1 cut(s) 1896
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 3 cut(s) 74, 1896, 2405
BstMAI GTCTC 4 cut(s) 551, 1358, 2282, 2655
BstNI CCWGG 6 cut(s) 1359, 1471, 1638, 1758, 2166, 2534
BstNSI RCATGY 3 cut(s) 188, 1190, 1916
BstSCI CCNGG 7 cut(s) 472, 1357, 1469, 1636, 1756, 2164, 2532
BstSFI CTRYAG 4 cut(s) 927, 1838, 2103, 2759
BstSLI GKGCMC 2 cut(s) 1456, 2064
BstUI CGCG 1 cut(s) 1896
BstV2I GAAGAC 1 cut(s) 868
BstX2I RGATCY 3 cut(s) 410, 1649, 2131
BstYI RGATCY 3 cut(s) 410, 1649, 2131
Bsu15I ATCGAT 1 cut(s) 1740
BsuI GTATCC 2 cut(s) 1156, 2551
BsuRI GGCC 5 cut(s) 223, 1636, 1960, 2062, 2140
BsuTUI ATCGAT 1 cut(s) 1740
BtgI CCRYGG 5 cut(s) 645, 1158, 1227, 2135, 2452
BtgZI GCGATG 1 cut(s) 1695
BtsCI GGATG 6 cut(s) 655, 664, 823, 1615, 2208, 2607
BtsI GCAGTG 2 cut(s) 1907, 2944
BtsIMutI CAGTG 4 cut(s) 1067, 1705, 1907, 2944
BveI ACCTGC 2 cut(s) 413, 2096
Cac8I GCNNGC 9 cut(s) 135, 709, 831, 835, 927, 1206, 1914, 1944, 2948
CaiI CAGNNNCTG 3 cut(s) 1470, 2569, 2681
CfoI GCGC 3 cut(s) 74, 1896, 2405
ClaI ATCGAT 1 cut(s) 1740
CseI GACGC 1 cut(s) 1304
Csp6I GTAC 8 cut(s) 332, 453, 600, 912, 1716, 2109, 2233, 2361
CviQI GTAC 8 cut(s) 332, 453, 600, 912, 1716, 2109, 2233, 2361
DdeI CTNAG 6 cut(s) 77, 752, 1674, 1829, 2628, 2910
DinI GGCGCC 1 cut(s) 73
DraI TTTAAA 1 cut(s) 1600
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 737, 1672
EarI CTCTTC 2 cut(s) 737, 1672
Eco130I CCWWGG 5 cut(s) 645, 1133, 1227, 2135, 2695
Eco24I GRGCYC 1 cut(s) 2064
Eco31I GGTCTC 3 cut(s) 551, 2282, 2655
Eco32I GATATC 1 cut(s) 2646
Eco47I GGWCC 7 cut(s) 362, 970, 1077, 2049, 2159, 2344, 2737
Eco57I CTGAAG 7 cut(s) 108, 438, 522, 1196, 1653, 2270, 2318
Eco72I CACGTG 1 cut(s) 2525
Eco88I CYCGRG 4 cut(s) 9, 2499, 2733, 2874
EcoO109I RGGNCCY 3 cut(s) 2049, 2061, 2159
EcoRI GAATTC 1 cut(s) 1180
EcoRII CCWGG 6 cut(s) 1357, 1469, 1636, 1756, 2164, 2532
EcoRV GATATC 1 cut(s) 2646
EcoT14I CCWWGG 5 cut(s) 645, 1133, 1227, 2135, 2695
EcoT22I ATGCAT 4 cut(s) 190, 1918, 2097, 2607
EcoT38I GRGCYC 1 cut(s) 2064
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 5 cut(s) 645, 1133, 1227, 2135, 2695
FaqI GGGAC 3 cut(s) 1799, 1981, 2750
FbaI TGATCA 4 cut(s) 589, 1212, 1819, 2035
FokI GGATG 6 cut(s) 662, 671, 830, 1622, 2195, 2614
FriOI GRGCYC 1 cut(s) 2064
FspBI CTAG 8 cut(s) 603, 1023, 1074, 1497, 1880, 2258, 2388, 2801
GlaI GCGC 3 cut(s) 73, 1895, 2404
GsaI CCCAGC 3 cut(s) 779, 1894, 2068
GsuI CTGGAG 3 cut(s) 492, 2148, 2936
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 5 cut(s) 223, 1636, 1960, 2062, 2140
HapII CCGG 2 cut(s) 473, 1723
HgaI GACGC 1 cut(s) 1304
HhaI GCGC 3 cut(s) 74, 1896, 2405
Hin1I GRCGYC 1 cut(s) 72
Hin6I GCGC 3 cut(s) 72, 1894, 2403
HinP1I GCGC 3 cut(s) 72, 1894, 2403
HpaII CCGG 2 cut(s) 473, 1723
HphI GGTGA 4 cut(s) 260, 870, 1106, 1346
Hpy166II GTNNAC 4 cut(s) 461, 1454, 1593, 2363
Hpy8I GTNNAC 4 cut(s) 461, 1454, 1593, 2363
Hpy99I CGWCG 1 cut(s) 1926
HpyCH4III ACNGT 9 cut(s) 1071, 1516, 1720, 1867, 2188, 2453, 2582, 2671, 2929
HpyCH4IV ACGT 4 cut(s) 787, 906, 2205, 2524
HpyF3I CTNAG 6 cut(s) 77, 752, 1674, 1829, 2628, 2910
HpySE526I ACGT 4 cut(s) 787, 906, 2205, 2524
Hsp92I GRCGYC 1 cut(s) 72
HspAI GCGC 3 cut(s) 72, 1894, 2403
KasI GGCGCC 1 cut(s) 71
Ksp22I TGATCA 4 cut(s) 589, 1212, 1819, 2035
LmnI GCTCC 4 cut(s) 511, 1229, 1687, 2955
LweI GCATC 8 cut(s) 197, 241, 391, 1360, 1492, 1929, 2592, 2614
MaeI CTAG 8 cut(s) 603, 1023, 1074, 1497, 1880, 2258, 2388, 2801
MaeII ACGT 4 cut(s) 787, 906, 2205, 2524
MaeIII GTNAC 5 cut(s) 883, 951, 1094, 1286, 1330
MbiI CCGCTC 1 cut(s) 2551
MflI RGATCY 3 cut(s) 410, 1649, 2131
MhlI GDGCHC 2 cut(s) 1456, 2064
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 2 cut(s) 1028, 1781
Mph1103I ATGCAT 4 cut(s) 190, 1918, 2097, 2607
MroXI GAANNNNTTC 3 cut(s) 1431, 1974, 2565
MslI CAYNNNNRTG 2 cut(s) 721, 1776
MspA1I CMGCKG 5 cut(s) 1174, 1673, 2102, 2678, 2774
MspI CCGG 2 cut(s) 473, 1723
MspR9I CCNGG 7 cut(s) 474, 1359, 1471, 1638, 1758, 2166, 2534
Mva1269I GAATGC 4 cut(s) 1451, 1589, 2018, 2202
MvaI CCWGG 6 cut(s) 1359, 1471, 1638, 1758, 2166, 2534
MvnI CGCG 1 cut(s) 1896
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 474
NcoI CCATGG 3 cut(s) 645, 1227, 2135
NlaIV GGNNCC 5 cut(s) 73, 1689, 2062, 2063, 2738
NmeAIII GCCGAG 3 cut(s) 73, 430, 1612
NmuCI GTSAC 2 cut(s) 951, 1094
NsiI ATGCAT 4 cut(s) 190, 1918, 2097, 2607
NspI RCATGY 3 cut(s) 188, 1190, 1916
OliI CACNNNNGTG 1 cut(s) 721
PaeI GCATGC 1 cut(s) 1916
PaeR7I CTCGAG 2 cut(s) 9, 2874
PaqCI CACCTGC 1 cut(s) 413
PasI CCCWGGG 2 cut(s) 1358, 1757
PciI ACATGT 1 cut(s) 1186
PctI GAATGC 4 cut(s) 1451, 1589, 2018, 2202
PdmI GAANNNNTTC 3 cut(s) 1431, 1974, 2565
PfeI GAWTC 9 cut(s) 1028, 1232, 1397, 1796, 2350, 2457, 2513, 2743, 2764
PfoI TCCNGGA 1 cut(s) 2164
PleI GAGTC 2 cut(s) 1027, 1781
PluTI GGCGCC 1 cut(s) 75
PmaCI CACGTG 1 cut(s) 2525
PmlI CACGTG 1 cut(s) 2525
PpsI GAGTC 2 cut(s) 1027, 1781
Ppu21I YACGTR 1 cut(s) 2525
PpuMI RGGWCCY 2 cut(s) 2049, 2159
PscI ACATGT 1 cut(s) 1186
PsiI TTATAA 1 cut(s) 1322
Psp5II RGGWCCY 2 cut(s) 2049, 2159
Psp6I CCWGG 6 cut(s) 1357, 1469, 1636, 1756, 2164, 2532
PspCI CACGTG 1 cut(s) 2525
PspFI CCCAGC 3 cut(s) 775, 1890, 2064
PspGI CCWGG 6 cut(s) 1357, 1469, 1636, 1756, 2164, 2532
PspN4I GGNNCC 5 cut(s) 73, 1689, 2062, 2063, 2738
PspOMI GGGCCC 1 cut(s) 2060
PspPPI RGGWCCY 2 cut(s) 2049, 2159
PspXI VCTCGAGB 1 cut(s) 2874
PstI CTGCAG 3 cut(s) 931, 2107, 2763
PstNI CAGNNNCTG 3 cut(s) 1470, 2569, 2681
PsuI RGATCY 3 cut(s) 410, 1649, 2131
PvuII CAGCTG 5 cut(s) 1174, 1673, 2102, 2678, 2774
RsaI GTAC 8 cut(s) 333, 454, 601, 913, 1717, 2110, 2234, 2362
RsaNI GTAC 8 cut(s) 332, 453, 600, 912, 1716, 2109, 2233, 2361
RseI CAYNNNNRTG 2 cut(s) 721, 1776
ScaI AGTACT 2 cut(s) 601, 2234
SchI GAGTC 2 cut(s) 1028, 1781
ScrFI CCNGG 7 cut(s) 474, 1359, 1471, 1638, 1758, 2166, 2534
SduI GDGCHC 2 cut(s) 1456, 2064
SfaNI GCATC 8 cut(s) 197, 241, 391, 1360, 1492, 1929, 2592, 2614
SfcI CTRYAG 4 cut(s) 927, 1838, 2103, 2759
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 2 cut(s) 9, 2874
SinI GGWCC 7 cut(s) 362, 970, 1077, 2049, 2159, 2344, 2737
SlaI CTCGAG 2 cut(s) 9, 2874
SmiMI CAYNNNNRTG 2 cut(s) 721, 1776
SmlI CTYRAG 5 cut(s) 9, 548, 719, 2874, 2885
SmoI CTYRAG 5 cut(s) 9, 548, 719, 2874, 2885
SpeI ACTAGT 1 cut(s) 602
SphI GCATGC 1 cut(s) 1916
SsiI CCGC 4 cut(s) 610, 1014, 1430, 2551
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 8 cut(s) 603, 1023, 1074, 1497, 1880, 2258, 2388, 2801
StyD4I CCNGG 7 cut(s) 472, 1357, 1469, 1636, 1756, 2164, 2532
StyI CCWWGG 5 cut(s) 645, 1133, 1227, 2135, 2695
TaaI ACNGT 9 cut(s) 1071, 1516, 1720, 1867, 2188, 2453, 2582, 2671, 2929
TaiI ACGT 4 cut(s) 790, 909, 2208, 2527
TaqI TCGA 5 cut(s) 10, 1740, 2485, 2746, 2875
TatI WGTACW 2 cut(s) 599, 2232
TauI GCSGC 2 cut(s) 612, 1016
TfiI GAWTC 9 cut(s) 1028, 1232, 1397, 1796, 2350, 2457, 2513, 2743, 2764
TscAI CASTG 4 cut(s) 1074, 1705, 1914, 2944
TseFI GTSAC 2 cut(s) 951, 1094
Tsp45I GTSAC 2 cut(s) 951, 1094
TspGWI ACGGA 4 cut(s) 681, 1175, 2533, 2626
TspRI CASTG 4 cut(s) 1074, 1705, 1914, 2944
VneI GTGCAC 1 cut(s) 1452
VpaK11BI GGWCC 7 cut(s) 362, 970, 1077, 2049, 2159, 2344, 2737
XapI RAATTY 3 cut(s) 1180, 1851, 2026
XbaI TCTAGA 1 cut(s) 1496
XceI RCATGY 3 cut(s) 188, 1190, 1916
XcmI CCANNNNNNNNNTGG 1 cut(s) 2702
XhoI CTCGAG 2 cut(s) 9, 2874
XmnI GAANNNNTTC 3 cut(s) 1431, 1974, 2565
XspI CTAG 8 cut(s) 603, 1023, 1074, 1497, 1880, 2258, 2388, 2801
ZrmI AGTACT 2 cut(s) 601, 2234
Zsp2I ATGCAT 4 cut(s) 190, 1918, 2097, 2607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.