MD08G1183900.v1.1

Monoacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016353
Physical Location & Seq
Forward (+)
22917700 .. 22921068
3369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1183900.v1.1.491

Sequence Viewer

Length: 954 bp
ATGCCATGCTTCAGCCTCACCCAGTCCAAAAACTCATGCCACCGGTCCACCTTCGCCCGAGCCGGCCTCCGGTCCGCAATCACCGACCTCAAAGACGGTGCCACCACCATGCACTGCTGGGTCCCACAGTCCCCAAACCCTTCCAAACCCAACCTCCTCCTCATACACGGCCTCGGCCTCAACGCAATGTGGCAGTTCGTAGACCTTCTCCGCCATGTCACCCCTCACTACAACGTCTACGTGCCGGATCTCGTCTTCTTCGGCGATTCCTACACGACCCGGCCAGACCGGTCCGAGTCGTTCCAGGCGGAGTGTGTGATGCAGGCGATGGAGGCGCACTCGGTGCGGCGGCTGAGCCTGGTGGGACTGAGCTACGGCGGGTTTGTTGCGTACAGCTTGGCTGCCAGGTACAAGGAGGCGGTGGAGAGGGTTGTGATATGCTGCGCCGCCGTGTGCCTGGAGGAGAAGGACCTCCAGGAAGGTGCGTTTCGGATCTCGGATTTGGATGAGGCGGCGGGGATTTTGACGCCGCAGACGCCGCAGAAGCTGAGGGAGTTGGTTAGGTACACGTTTTTCAGGCCACCTCCAGTTGGGTTGTTGCCCTCTTGCTTGCTCATGGATTTCATTGAGGCGATGTTCACCGAATTTGTGCAAGAGAAGAAAGAGTTGATCCGGGCCGTCCCCCAAAACCGTAAACTTTCAGAACTCCCCAAGATTCCTCAGCCAACCTTCATAATTTGGGGAGAACATGATCAAGTGTTTCCAGTTGAGTATGCTCACAAATTAAAAAGGCACGTCGGAGAAAACGCTCAGCTTGTAGTAATCAAGGATGCAGGGCATGCACTAAACGCAGAGAAGCCCAAGGAGTACCACAAGCACATCAAGTCCTTCTTAGTCGATTCGCTGCCCCGTTCCCCTCCGATAACTCCATCTGCTATACCAGTCACTCACTAA

Protein Analysis

318

Amino Acids

35.72

Weight (kDa)

8.09

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 50 - 156 1.1e-14 alpha/beta hydrolase fold
Hydrolase_4 PF12146 50 - 286 1.4e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 52 - 290 3.3e-20 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 226 - 287 4e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016353)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37170
malus_domestica MD08G1183900.v1.1 MD15G1370400.v1.1
prunus_persica Prupe.1G518000_v2.0.a1
pyrus_communis pycom08g15770 pycom15g33210
rosa_chinensis RchiOBHm_Chr7g0238751
rosa_laevigata RLG00000000815
rosa_multiflora Rmu_sc0010914.1_g000009
rosa_roxburghii Rroxscaffold_3G00221580
rosa_rugosa Rorug07G0323400
rosa_samantha Rh7AG477700 Rh7CG495700 Rh7DG462700
rosa_wichuraiana Rw7G039870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccI GTMKAC 2 cut(s) 201, 237
AclWI GGATC 3 cut(s) 255, 500, 664
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 644
AcyI GRCGYC 2 cut(s) 527, 536
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 4 cut(s) 392, 410, 566, 869
AfiI CCNNNNNNNGG 2 cut(s) 69, 590
AflIII ACRYGT 1 cut(s) 567
AgeI ACCGGT 2 cut(s) 42, 288
AjiI CACGTC 1 cut(s) 796
AjnI CCWGG 5 cut(s) 303, 357, 404, 456, 474
AluBI AGCT 4 cut(s) 372, 396, 547, 814
AluI AGCT 4 cut(s) 372, 396, 547, 814
AlwI GGATC 3 cut(s) 255, 500, 664
AlwNI CAGNNNCTG 1 cut(s) 547
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 6 cut(s) 64, 169, 175, 281, 578, 675
ApeKI GCWGC 3 cut(s) 401, 441, 904
ApoI RAATTY 1 cut(s) 644
AsiGI ACCGGT 2 cut(s) 42, 288
AspLEI GCGC 2 cut(s) 337, 446
AspS9I GGNCC 6 cut(s) 45, 72, 121, 291, 469, 675
AsuC2I CCSGG 2 cut(s) 280, 674
AsuHPI GGTGA 4 cut(s) 10, 73, 211, 631
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 5 cut(s) 45, 72, 121, 291, 469
BanI GGYRCC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 247
BbvCI CCTCAGC 2 cut(s) 548, 720
BbvI GCAGC 3 cut(s) 388, 428, 891
BccI CCATC 2 cut(s) 322, 937
BceAI ACGGC 4 cut(s) 184, 391, 434, 662
BciT130I CCWGG 5 cut(s) 305, 359, 406, 458, 476
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 2 cut(s) 280, 674
BisI GCNGC 9 cut(s) 347, 350, 402, 442, 447, 513, 530, 539, 905
BlpI GCTNAGC 2 cut(s) 353, 810
BlsI GCNGC 9 cut(s) 348, 351, 403, 443, 448, 514, 531, 540, 906
Bme1390I CCNGG 7 cut(s) 280, 305, 359, 406, 458, 476, 674
Bme18I GGWCC 5 cut(s) 45, 72, 121, 291, 469
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 796
BmgT120I GGNCC 6 cut(s) 45, 72, 121, 291, 469, 675
BmiI GGNNCC 3 cut(s) 100, 122, 123
BmrFI CCNGG 7 cut(s) 280, 305, 359, 406, 458, 476, 674
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 2 cut(s) 309, 820
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 247
BplI GAGNNNNNCTC 4 cut(s) 51, 83, 323, 355
BpmI CTGGAG 3 cut(s) 458, 479, 570
Bpu10I CCTNAGC 2 cut(s) 548, 720
Bpu1102I GCTNAGC 2 cut(s) 353, 810
BpuMI CCSGG 2 cut(s) 280, 674
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 527, 536
BsaJI CCNNGG 2 cut(s) 172, 861
BsaWI WCCGGW 3 cut(s) 42, 69, 288
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 590
Bse118I RCCGGY 3 cut(s) 42, 62, 288
Bse1I ACTGG 4 cut(s) 22, 587, 764, 941
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 5 cut(s) 305, 359, 406, 458, 476
BseDI CCNNGG 2 cut(s) 172, 861
BseGI GGATG 2 cut(s) 511, 835
BseLI CCNNNNNNNGG 2 cut(s) 69, 590
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 5 cut(s) 344, 359, 539, 734, 824
BseNI ACTGG 4 cut(s) 22, 587, 764, 941
BseRI GAGGAG 3 cut(s) 146, 149, 476
BseXI GCAGC 3 cut(s) 388, 428, 891
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 6 cut(s) 66, 171, 177, 283, 580, 677
BshNI GGYRCC 1 cut(s) 98
BshTI ACCGGT 2 cut(s) 42, 288
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 7 cut(s) 43, 63, 70, 245, 280, 289, 673
BslFI GGGAC 4 cut(s) 107, 115, 378, 665
BslI CCNNNNNNNGG 2 cut(s) 69, 590
BsmFI GGGAC 4 cut(s) 107, 115, 378, 665
BsnI GGCC 6 cut(s) 66, 171, 177, 283, 580, 677
BsoBI CYCGRG 1 cut(s) 57
Bsp143I GATC 4 cut(s) 247, 492, 669, 751
Bsp1720I GCTNAGC 2 cut(s) 353, 810
BspANI GGCC 6 cut(s) 66, 171, 177, 283, 580, 677
BspCNI CTCAG 5 cut(s) 345, 360, 540, 733, 823
BspLI GGNNCC 3 cut(s) 100, 122, 123
BspPI GGATC 3 cut(s) 255, 500, 664
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 3 cut(s) 42, 62, 288
BsrI ACTGG 4 cut(s) 22, 587, 764, 941
BssAI RCCGGY 3 cut(s) 42, 62, 288
BssECI CCNNGG 2 cut(s) 172, 861
BssMI GATC 4 cut(s) 247, 492, 669, 751
BssNI GRCGYC 2 cut(s) 527, 536
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 5 cut(s) 305, 359, 406, 458, 476
Bst4CI ACNGT 3 cut(s) 98, 129, 692
BstACI GRCGYC 2 cut(s) 527, 536
BstAPI GCANNNNNTGC 2 cut(s) 343, 839
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 4 cut(s) 64, 324, 611, 840
BstDEI CTNAG 6 cut(s) 353, 368, 548, 720, 810, 892
BstF5I GGATG 2 cut(s) 511, 835
BstHHI GCGC 2 cut(s) 337, 446
BstKTI GATC 4 cut(s) 250, 495, 672, 754
BstMBI GATC 4 cut(s) 247, 492, 669, 751
BstMWI GCNNNNNNNGC 7 cut(s) 332, 343, 535, 538, 544, 839, 848
BstNI CCWGG 5 cut(s) 305, 359, 406, 458, 476
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 7 cut(s) 278, 303, 357, 404, 456, 474, 672
BstV1I GCAGC 3 cut(s) 388, 428, 891
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 247, 492
BstYI RGATCY 2 cut(s) 247, 492
BsuRI GGCC 6 cut(s) 66, 171, 177, 283, 580, 677
BtgZI GCGATG 2 cut(s) 341, 647
BtrI CACGTC 1 cut(s) 796
BtsCI GGATG 2 cut(s) 511, 835
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 4 cut(s) 64, 324, 611, 840
CaiI CAGNNNCTG 1 cut(s) 547
CfoI GCGC 2 cut(s) 337, 446
Cfr10I RCCGGY 3 cut(s) 42, 62, 288
Cfr13I GGNCC 6 cut(s) 45, 72, 121, 291, 469, 675
CpoI CGGWCCG 2 cut(s) 72, 291
CseI GACGC 2 cut(s) 535, 544
Csp6I GTAC 4 cut(s) 391, 409, 565, 868
CspAI ACCGGT 2 cut(s) 42, 288
CspI CGGWCCG 2 cut(s) 72, 291
CviAII CATG 7 cut(s) 6, 36, 109, 215, 616, 749, 839
CviQI GTAC 4 cut(s) 391, 409, 565, 868
DdeI CTNAG 6 cut(s) 353, 368, 548, 720, 810, 892
DpnI GATC 4 cut(s) 249, 494, 671, 753
DpnII GATC 4 cut(s) 247, 492, 669, 751
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 281
EciI GGCGGA 2 cut(s) 200, 323
Eco130I CCWWGG 1 cut(s) 861
Eco47I GGWCC 5 cut(s) 45, 72, 121, 291, 469
Eco88I CYCGRG 1 cut(s) 57
EcoO109I RGGNCCY 2 cut(s) 121, 469
EcoRII CCWGG 5 cut(s) 303, 357, 404, 456, 474
EcoT14I CCWWGG 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 7 cut(s) 9, 39, 112, 218, 619, 752, 842
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 4 cut(s) 107, 115, 378, 665
FatI CATG 7 cut(s) 5, 35, 108, 214, 615, 748, 838
FauI CCCGC 2 cut(s) 371, 508
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 2 cut(s) 201, 237
Fnu4HI GCNGC 9 cut(s) 347, 350, 402, 442, 447, 513, 530, 539, 905
FokI GGATG 2 cut(s) 518, 842
Fsp4HI GCNGC 9 cut(s) 347, 350, 402, 442, 447, 513, 530, 539, 905
GlaI GCGC 2 cut(s) 336, 445
GluI GCNGC 9 cut(s) 347, 350, 402, 442, 447, 513, 530, 539, 905
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 3 cut(s) 458, 479, 570
HaeIII GGCC 6 cut(s) 66, 171, 177, 283, 580, 677
HapII CCGG 7 cut(s) 43, 63, 70, 245, 280, 289, 673
HgaI GACGC 2 cut(s) 535, 544
HhaI GCGC 2 cut(s) 337, 446
Hin1I GRCGYC 2 cut(s) 527, 536
Hin1II CATG 7 cut(s) 9, 39, 112, 218, 619, 752, 842
Hin6I GCGC 2 cut(s) 335, 444
HinP1I GCGC 2 cut(s) 335, 444
HinfI GANTC 4 cut(s) 266, 296, 715, 899
HpaII CCGG 7 cut(s) 43, 63, 70, 245, 280, 289, 673
HphI GGTGA 4 cut(s) 10, 73, 211, 631
Hpy166II GTNNAC 6 cut(s) 48, 202, 238, 567, 639, 695
Hpy188I TCNGA 6 cut(s) 295, 492, 499, 703, 800, 921
Hpy8I GTNNAC 6 cut(s) 48, 202, 238, 567, 639, 695
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 7 cut(s) 61, 150, 215, 460, 473, 739, 898
HpyCH4III ACNGT 3 cut(s) 98, 129, 692
HpyCH4IV ACGT 4 cut(s) 234, 240, 569, 795
HpyCH4V TGCA 5 cut(s) 112, 322, 652, 833, 842
HpyF10VI GCNNNNNNNGC 7 cut(s) 332, 343, 535, 538, 544, 839, 848
HpyF3I CTNAG 6 cut(s) 353, 368, 548, 720, 810, 892
HpySE526I ACGT 4 cut(s) 234, 240, 569, 795
Hsp92I GRCGYC 2 cut(s) 527, 536
Hsp92II CATG 7 cut(s) 9, 39, 112, 218, 619, 752, 842
HspAI GCGC 2 cut(s) 335, 444
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 4 cut(s) 247, 492, 669, 751
Lsp1109I GCAGC 3 cut(s) 388, 428, 891
LweI GCATC 2 cut(s) 309, 820
MaeII ACGT 4 cut(s) 234, 240, 569, 795
MaeIII GTNAC 2 cut(s) 217, 943
MalI GATC 4 cut(s) 249, 494, 671, 753
MboI GATC 4 cut(s) 247, 492, 669, 751
MboII GAAGA 3 cut(s) 247, 250, 670
MflI RGATCY 2 cut(s) 247, 492
MluCI AATT 3 cut(s) 644, 735, 782
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 778
MroNI GCCGGC 1 cut(s) 62
MseI TTAA 1 cut(s) 785
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 7 cut(s) 43, 63, 70, 245, 280, 289, 673
MspR9I CCNGG 7 cut(s) 280, 305, 359, 406, 458, 476, 674
MvaI CCWGG 5 cut(s) 305, 359, 406, 458, 476
MwoI GCNNNNNNNGC 7 cut(s) 332, 343, 535, 538, 544, 839, 848
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 2 cut(s) 280, 674
NdeII GATC 4 cut(s) 247, 492, 669, 751
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 7 cut(s) 9, 39, 112, 218, 619, 752, 842
NlaIV GGNNCC 3 cut(s) 100, 122, 123
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 217, 943
NspI RCATGY 1 cut(s) 842
PaeI GCATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 64
PfeI GAWTC 3 cut(s) 266, 715, 899
PfoI TCCNGGA 1 cut(s) 474
PinAI ACCGGT 2 cut(s) 42, 288
PkrI GCNGC 9 cut(s) 348, 351, 403, 443, 448, 514, 531, 540, 906
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Ppu21I YACGTR 1 cut(s) 241
PpuMI RGGWCCY 2 cut(s) 121, 469
Psp5II RGGWCCY 2 cut(s) 121, 469
Psp6I CCWGG 5 cut(s) 303, 357, 404, 456, 474
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 5 cut(s) 303, 357, 404, 456, 474
PspN4I GGNNCC 3 cut(s) 100, 122, 123
PspPI GGNCC 6 cut(s) 45, 72, 121, 291, 469, 675
PspPPI RGGWCCY 2 cut(s) 121, 469
PstNI CAGNNNCTG 1 cut(s) 547
PsuI RGATCY 2 cut(s) 247, 492
RsaI GTAC 4 cut(s) 392, 410, 566, 869
RsaNI GTAC 4 cut(s) 391, 409, 565, 868
RseI CAYNNNNRTG 1 cut(s) 107
Rsr2I CGGWCCG 2 cut(s) 72, 291
RsrII CGGWCCG 2 cut(s) 72, 291
SaqAI TTAA 1 cut(s) 785
SatI GCNGC 9 cut(s) 347, 350, 402, 442, 447, 513, 530, 539, 905
Sau3AI GATC 4 cut(s) 247, 492, 669, 751
Sau96I GGNCC 6 cut(s) 45, 72, 121, 291, 469, 675
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 7 cut(s) 280, 305, 359, 406, 458, 476, 674
SfaNI GCATC 2 cut(s) 309, 820
SinI GGWCC 5 cut(s) 45, 72, 121, 291, 469
SmiMI CAYNNNNRTG 1 cut(s) 107
SphI GCATGC 1 cut(s) 842
Sse9I AATT 3 cut(s) 644, 735, 782
StyD4I CCNGG 7 cut(s) 278, 303, 357, 404, 456, 474, 672
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 98, 129, 692
TaiI ACGT 4 cut(s) 237, 243, 572, 798
TaqI TCGA 1 cut(s) 897
TasI AATT 3 cut(s) 644, 735, 782
TauI GCSGC 6 cut(s) 349, 352, 449, 515, 532, 541
TfiI GAWTC 3 cut(s) 266, 715, 899
Tru1I TTAA 1 cut(s) 785
Tru9I TTAA 1 cut(s) 785
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 2 cut(s) 217, 943
TseI GCWGC 3 cut(s) 401, 441, 904
Tsp45I GTSAC 2 cut(s) 217, 943
TspDTI ATGAA 2 cut(s) 613, 721
TspRI CASTG 1 cut(s) 119
VpaK11BI GGWCC 5 cut(s) 45, 72, 121, 291, 469
XapI RAATTY 1 cut(s) 644
XceI RCATGY 1 cut(s) 842
XmiI GTMKAC 2 cut(s) 201, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.