Prupe.1G518000_v2.0.a1

Monoacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016353
Physical Location & Seq
Forward (+)
42565491 .. 42567875
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G518000.1

Sequence Viewer

Length: 963 bp
ATGCCATGCTTCAGCTTCACCGAGTCAAAAAATTCGTGCCACCGGTCCACCTTCGCCCGAGCCGGTCTCCGGTCCGCAATCACAGACATCAAAGATGGCGGTACCACCATGCACTGCTGGGTCCCGCAGTCCCCAAACCCGTCCAAGCGCAACCTCCTCCTCATCCACGGCCTCGGAGTCAACGCGATGTGGCAATTCGTTGACCTCCTCCGCCACGTCACCCCCCACTTCAACGTCTACGTCCCCGACCTCCTCTTCTTCGGCGAGTCGTCCACTACCCGGCCGGACCGGTCCGAGTCGTTCCAGGCCGAGTGCGTGATGCGGGTCATGGAGGCCCACTCGGTGCGGAGGCTGAGCCTGGTGGGGCTGAGCTATGGCGGGTTTGTGGGGTACAGCCTGGCGGCCAATTACAGGGAGTCGGTGGAGAGGGTTGTGATATGCTGCGCCGCGGTGTGCTTGGAGGAGAAAGACATCCGGGAAGGCGTGTTTCCGATATCGGATTTGGACGAGGCTGCGAGCATTTTGGTGCCGCAGACGCCGCAGAAGCTTAGGGAGTTGATCAAGTACACTTTTTTCAAGCCGCCTCCTCTTGGGTTGATCCCCTCTTGCTTGCTTATGGATTTCATTGAGGCAATGTACACTGAACATGTGAAAGAGCAGAAAGAGTTGATTATGGCCGTCCCCAAAAACCGAAAACTTTCAGACCTTCCCAAGATTCCACAGCCAACCCTTATAATATGGGGAGAGCATGATCAAGTATTTCCGCTAGAATTTGCATACAAATTAAAAAGGCACTTAGGGGAGAACGCTGAGCTTGTGGTGATAAAGGATGCAGGACATGCAATTAACGTACAAAAGCCCAAGGAGTACTACAACCACATCAAGTCCTTCTTACTCGACATGCAGCAGCCATCTGCTAATAAACATCAACAACATTCAAAACCACTTCAATCACTGACATGA

Protein Analysis

321

Amino Acids

36.31

Weight (kDa)

8.07

Isoelectric Point (pI)

50.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016353)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37170
malus_domestica MD08G1183900.v1.1 MD15G1370400.v1.1
prunus_persica Prupe.1G518000_v2.0.a1
pyrus_communis pycom08g15770 pycom15g33210
rosa_chinensis RchiOBHm_Chr7g0238751
rosa_laevigata RLG00000000815
rosa_multiflora Rmu_sc0010914.1_g000009
rosa_roxburghii Rroxscaffold_3G00221580
rosa_rugosa Rorug07G0323400
rosa_samantha Rh7AG477700 Rh7CG495700 Rh7DG462700
rosa_wichuraiana Rw7G039870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 101, 526
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 185, 449
AclWI GGATC 1 cut(s) 592
AcoI YGGCCR 3 cut(s) 281, 402, 675
AcsI RAATTY 2 cut(s) 31, 770
AcyI GRCGYC 1 cut(s) 536
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 6 cut(s) 103, 392, 566, 638, 852, 869
AfiI CCNNNNNNNGG 4 cut(s) 69, 279, 411, 590
AflIII ACRYGT 1 cut(s) 646
AgeI ACCGGT 2 cut(s) 42, 288
AgsI TTSAA 4 cut(s) 232, 577, 939, 950
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 3 cut(s) 303, 357, 396
AluBI AGCT 4 cut(s) 15, 372, 547, 814
AluI AGCT 4 cut(s) 15, 372, 547, 814
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 1 cut(s) 592
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 6 cut(s) 169, 281, 306, 333, 402, 675
ApeKI GCWGC 4 cut(s) 441, 512, 904, 907
ApoI RAATTY 2 cut(s) 31, 770
AsiGI ACCGGT 2 cut(s) 42, 288
Asp700I GAANNNNTTC 1 cut(s) 697
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 150, 446
AspS9I GGNCC 6 cut(s) 45, 72, 121, 286, 291, 334
AsuC2I CCSGG 2 cut(s) 280, 476
AsuHPI GGTGA 3 cut(s) 10, 211, 832
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 5 cut(s) 45, 72, 121, 286, 291
BanI GGYRCC 2 cut(s) 101, 526
BbvI GCAGC 4 cut(s) 428, 499, 916, 919
BccI CCATC 2 cut(s) 89, 919
BceAI ACGGC 2 cut(s) 184, 662
BciT130I CCWGG 3 cut(s) 305, 359, 398
BclI TGATCA 2 cut(s) 558, 751
BcnI CCSGG 2 cut(s) 280, 476
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 767
BisI GCNGC 9 cut(s) 402, 442, 447, 513, 530, 539, 581, 905, 908
BlpI GCTNAGC 3 cut(s) 353, 368, 810
BlsI GCNGC 9 cut(s) 403, 443, 448, 514, 531, 540, 582, 906, 909
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 5 cut(s) 280, 305, 359, 398, 476
Bme18I GGWCC 5 cut(s) 45, 72, 121, 286, 291
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 6 cut(s) 45, 72, 121, 286, 291, 334
BmiI GGNNCC 4 cut(s) 103, 122, 123, 528
BmrFI CCNGG 5 cut(s) 280, 305, 359, 398, 476
BmsI GCATC 2 cut(s) 309, 820
BplI GAGNNNNNCTC 4 cut(s) 51, 83, 323, 355
Bpu10I CCTNAGC 1 cut(s) 548
Bpu1102I GCTNAGC 3 cut(s) 353, 368, 810
BpuMI CCSGG 2 cut(s) 280, 476
BsaHI GRCGYC 1 cut(s) 536
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 4 cut(s) 166, 172, 447, 861
BsaWI WCCGGW 3 cut(s) 42, 69, 288
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 279, 411, 590
Bse118I RCCGGY 3 cut(s) 42, 62, 288
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 3 cut(s) 305, 359, 398
BseDI CCNNGG 4 cut(s) 166, 172, 447, 861
BseGI GGATG 3 cut(s) 162, 471, 835
BseLI CCNNNNNNNGG 4 cut(s) 69, 279, 411, 590
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 344, 359, 801
BseRI GAGGAG 6 cut(s) 146, 149, 197, 242, 476, 576
BseX3I CGGCCG 1 cut(s) 281
BseXI GCAGC 4 cut(s) 428, 499, 916, 919
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 185, 449
Bsh1285I CGRYCG 1 cut(s) 284
BshFI GGCC 6 cut(s) 171, 283, 308, 335, 404, 677
BshNI GGYRCC 2 cut(s) 101, 526
BshTI ACCGGT 2 cut(s) 42, 288
BsiEI CGRYCG 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 7 cut(s) 43, 63, 70, 280, 284, 289, 475
BslFI GGGAC 4 cut(s) 107, 115, 227, 665
BslI CCNNNNNNNGG 4 cut(s) 69, 279, 411, 590
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 4 cut(s) 107, 115, 227, 665
BsnI GGCC 6 cut(s) 171, 283, 308, 335, 404, 677
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 3 cut(s) 558, 597, 751
Bsp1720I GCTNAGC 3 cut(s) 353, 368, 810
BspANI GGCC 6 cut(s) 171, 283, 308, 335, 404, 677
BspCNI CTCAG 3 cut(s) 345, 360, 802
BspFNI CGCG 2 cut(s) 185, 449
BspLI GGNNCC 4 cut(s) 103, 122, 123, 528
BspPI GGATC 1 cut(s) 592
BspT107I GGYRCC 2 cut(s) 101, 526
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 639
BsrFI RCCGGY 3 cut(s) 42, 62, 288
BsrGI TGTACA 1 cut(s) 636
BssAI RCCGGY 3 cut(s) 42, 62, 288
BssECI CCNNGG 4 cut(s) 166, 172, 447, 861
BssMI GATC 3 cut(s) 558, 597, 751
BssNI GRCGYC 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 3 cut(s) 305, 359, 398
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 517, 611
BstDEI CTNAG 5 cut(s) 353, 368, 548, 796, 810
BstDSI CCRYGG 2 cut(s) 166, 447
BstF5I GGATG 3 cut(s) 162, 471, 835
BstFNI CGCG 2 cut(s) 185, 449
BstHHI GCGC 2 cut(s) 150, 446
BstKTI GATC 3 cut(s) 561, 600, 754
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 3 cut(s) 558, 597, 751
BstMCI CGRYCG 1 cut(s) 284
BstMWI GCNNNNNNNGC 4 cut(s) 535, 538, 544, 839
BstNI CCWGG 3 cut(s) 305, 359, 398
BstNSI RCATGY 3 cut(s) 650, 842, 904
BstSCI CCNGG 5 cut(s) 278, 303, 357, 396, 474
BstUI CGCG 2 cut(s) 185, 449
BstV1I GCAGC 4 cut(s) 428, 499, 916, 919
BstZI CGGCCG 1 cut(s) 281
BsuRI GGCC 6 cut(s) 171, 283, 308, 335, 404, 677
BtgI CCRYGG 2 cut(s) 166, 447
BtgZI GCGATG 1 cut(s) 200
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 162, 471, 835
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 3 cut(s) 112, 639, 953
Cac8I GCNNGC 2 cut(s) 517, 611
CfoI GCGC 2 cut(s) 150, 446
Cfr10I RCCGGY 3 cut(s) 42, 62, 288
Cfr13I GGNCC 6 cut(s) 45, 72, 121, 286, 291, 334
Cfr42I CCGCGG 1 cut(s) 450
CpoI CGGWCCG 3 cut(s) 72, 286, 291
CseI GACGC 1 cut(s) 544
Csp6I GTAC 6 cut(s) 102, 391, 565, 637, 851, 868
CspAI ACCGGT 2 cut(s) 42, 288
CspI CGGWCCG 3 cut(s) 72, 286, 291
CviAII CATG 8 cut(s) 6, 109, 328, 647, 749, 839, 901, 960
CviQI GTAC 6 cut(s) 102, 391, 565, 637, 851, 868
DdeI CTNAG 5 cut(s) 353, 368, 548, 796, 810
DpnI GATC 3 cut(s) 560, 599, 753
DpnII GATC 3 cut(s) 558, 597, 751
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 3 cut(s) 281, 402, 675
EagI CGGCCG 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 200
EclXI CGGCCG 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 5 cut(s) 45, 72, 121, 286, 291
Eco52I CGGCCG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 303, 357, 396
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 8 cut(s) 9, 112, 331, 650, 752, 842, 904, 963
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 4 cut(s) 107, 115, 227, 665
FatI CATG 8 cut(s) 5, 108, 327, 646, 748, 838, 900, 959
FauI CCCGC 3 cut(s) 132, 315, 371
FbaI TGATCA 2 cut(s) 558, 751
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 9 cut(s) 402, 442, 447, 513, 530, 539, 581, 905, 908
FokI GGATG 3 cut(s) 149, 458, 842
Fsp4HI GCNGC 9 cut(s) 402, 442, 447, 513, 530, 539, 581, 905, 908
FspBI CTAG 1 cut(s) 767
GlaI GCGC 2 cut(s) 149, 445
GluI GCNGC 9 cut(s) 402, 442, 447, 513, 530, 539, 581, 905, 908
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 6 cut(s) 171, 283, 308, 335, 404, 677
HapII CCGG 7 cut(s) 43, 63, 70, 280, 284, 289, 475
HgaI GACGC 1 cut(s) 544
HhaI GCGC 2 cut(s) 150, 446
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 8 cut(s) 9, 112, 331, 650, 752, 842, 904, 963
Hin6I GCGC 2 cut(s) 148, 444
HinP1I GCGC 2 cut(s) 148, 444
HincII GTYRAC 2 cut(s) 181, 202
HindII GTYRAC 2 cut(s) 181, 202
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 6 cut(s) 23, 177, 266, 296, 416, 715
HpaII CCGG 7 cut(s) 43, 63, 70, 280, 284, 289, 475
HphI GGTGA 3 cut(s) 10, 211, 832
Hpy166II GTNNAC 7 cut(s) 48, 181, 202, 238, 273, 567, 639
Hpy188I TCNGA 5 cut(s) 176, 295, 492, 499, 703
Hpy8I GTNNAC 7 cut(s) 48, 181, 202, 238, 273, 567, 639
HpyAV CCTTC 4 cut(s) 61, 473, 716, 898
HpyCH4IV ACGT 4 cut(s) 216, 234, 240, 849
HpyCH4V TGCA 5 cut(s) 112, 776, 833, 842, 904
HpyF10VI GCNNNNNNNGC 4 cut(s) 535, 538, 544, 839
HpyF3I CTNAG 5 cut(s) 353, 368, 548, 796, 810
HpySE526I ACGT 4 cut(s) 216, 234, 240, 849
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 8 cut(s) 9, 112, 331, 650, 752, 842, 904, 963
HspAI GCGC 2 cut(s) 148, 444
KflI GGGWCCC 1 cut(s) 121
KpnI GGTACC 1 cut(s) 105
Ksp22I TGATCA 2 cut(s) 558, 751
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 3 cut(s) 558, 597, 751
Lsp1109I GCAGC 4 cut(s) 428, 499, 916, 919
LweI GCATC 2 cut(s) 309, 820
MaeI CTAG 1 cut(s) 767
MaeII ACGT 4 cut(s) 216, 234, 240, 849
MaeIII GTNAC 1 cut(s) 217
MalI GATC 3 cut(s) 560, 599, 753
MboI GATC 3 cut(s) 558, 597, 751
MboII GAAGA 2 cut(s) 247, 250
MluCI AATT 6 cut(s) 31, 194, 406, 770, 782, 843
MlyI GAGTC 5 cut(s) 32, 186, 275, 305, 425
MroXI GAANNNNTTC 1 cut(s) 697
MseI TTAA 2 cut(s) 785, 846
MslI CAYNNNNRTG 2 cut(s) 524, 958
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 7 cut(s) 43, 63, 70, 280, 284, 289, 475
MspR9I CCNGG 5 cut(s) 280, 305, 359, 398, 476
MvaI CCWGG 3 cut(s) 305, 359, 398
MvnI CGCG 2 cut(s) 185, 449
MwoI GCNNNNNNNGC 4 cut(s) 535, 538, 544, 839
NciI CCSGG 2 cut(s) 280, 476
NdeII GATC 3 cut(s) 558, 597, 751
NlaIII CATG 8 cut(s) 9, 112, 331, 650, 752, 842, 904, 963
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 528
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 3 cut(s) 650, 842, 904
PciI ACATGT 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 697
PfeI GAWTC 1 cut(s) 715
PfoI TCCNGGA 1 cut(s) 474
PinAI ACCGGT 2 cut(s) 42, 288
PkrI GCNGC 9 cut(s) 403, 443, 448, 514, 531, 540, 582, 906, 909
PleI GAGTC 5 cut(s) 31, 185, 274, 304, 424
PpsI GAGTC 5 cut(s) 31, 185, 274, 304, 424
PpuMI RGGWCCY 1 cut(s) 121
PscI ACATGT 1 cut(s) 646
PsiI TTATAA 1 cut(s) 734
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 3 cut(s) 303, 357, 396
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 3 cut(s) 303, 357, 396
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 528
PspPI GGNCC 6 cut(s) 45, 72, 121, 286, 291, 334
PspPPI RGGWCCY 1 cut(s) 121
RsaI GTAC 6 cut(s) 103, 392, 566, 638, 852, 869
RsaNI GTAC 6 cut(s) 102, 391, 565, 637, 851, 868
RseI CAYNNNNRTG 2 cut(s) 524, 958
Rsr2I CGGWCCG 3 cut(s) 72, 286, 291
RsrII CGGWCCG 3 cut(s) 72, 286, 291
SacII CCGCGG 1 cut(s) 450
SaqAI TTAA 2 cut(s) 785, 846
SatI GCNGC 9 cut(s) 402, 442, 447, 513, 530, 539, 581, 905, 908
Sau3AI GATC 3 cut(s) 558, 597, 751
Sau96I GGNCC 6 cut(s) 45, 72, 121, 286, 291, 334
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 5 cut(s) 32, 186, 275, 305, 425
ScrFI CCNGG 5 cut(s) 280, 305, 359, 398, 476
SfaNI GCATC 2 cut(s) 309, 820
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 5 cut(s) 45, 72, 121, 286, 291
SmiMI CAYNNNNRTG 2 cut(s) 524, 958
Sse9I AATT 6 cut(s) 31, 194, 406, 770, 782, 843
SspMI CTAG 1 cut(s) 767
StyD4I CCNGG 5 cut(s) 278, 303, 357, 396, 474
StyI CCWWGG 1 cut(s) 861
TaiI ACGT 4 cut(s) 219, 237, 243, 852
TaqI TCGA 1 cut(s) 897
TasI AATT 6 cut(s) 31, 194, 406, 770, 782, 843
TatI WGTACW 3 cut(s) 564, 636, 867
TauI GCSGC 5 cut(s) 404, 449, 532, 541, 583
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 2 cut(s) 785, 846
Tru9I TTAA 2 cut(s) 785, 846
TscAI CASTG 3 cut(s) 119, 646, 960
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 4 cut(s) 441, 512, 904, 907
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 613
TspRI CASTG 3 cut(s) 119, 646, 960
VpaK11BI GGWCC 5 cut(s) 45, 72, 121, 286, 291
XapI RAATTY 2 cut(s) 31, 770
XceI RCATGY 3 cut(s) 650, 842, 904
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 697
XspI CTAG 1 cut(s) 767
ZrmI AGTACT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.