Rh7CG495700

Monoacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016353
Physical Location & Seq
Reverse (-)
66590507 .. 66592852
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG495700.1

Sequence Viewer

Length: 939 bp
ATGCCATGCTTCAGCTTCACCCAGTCAAAGAACTCCTGCTTCCGGTCCAACTTCATCCGGGCCGGCCTCCGGTCCACCGTCACGGACCTCAGAGACGGCGGCACCACCATGCAGTGCTGGGTCCCCAAATCCCCAAACCCCTCCAAACCCAACCTCCTCCTCCTCCACGGCCTCGGCGTCAACGCAATGTGGCAGTTCGCTGACCTCATCCGCCACGTCACCCCTCACTACAACGTCTACGTCCCCGACCTCCTCTTCTTCGGCGACTCCTCCACCACCCGACCCGACCGCTCCGAGACGTTCCAGGCCGAGTGCGTCATGCGGGTCATGGAGGCCCACTCCGTCCGGGCCTTCAGCCTCGTGGGCCTCAGCTACGGCGGCTTCGTCGGGTACAGGCTGGCGGCCCAGTACAAGGAGGCGGTTGAGAGGGTGGTGATTTGCTGCGCCGCGGTGTGTATGGAGGAGAAGGACCTGCAGGAAAGAGTGTTTAGGGTTTCGGATTTGGACGAGGCCGCCAGCATTTTGGTGCCGCAGACGCCGGACAAGCTCCGGGAGCTTGTCAAGTACACTTTTTTCAGGCCGCCGCCGTTGGGGTTTATGCCGTCGTGCTTGCTCAGAGATTTCATCGAGACAATGTTCACAGATTTTGTTCAAGAGAAGAAAGAGCTGATCATGGCCATCCCTAAAAACCGAAAACTTTCCGACCTTCCCAAGATTCCTCAGCCAACTCTTATAATCTGGGGAGAGCATGATAATGTATTTCCCTTGGAATATGCCCACAAACTAAAAATGCACTTGGGGGAGAATGCTCAGCTTGCAGTGATCAAGGATGCAGGGCACGCGATAAACGCTGAAAAGTGCAAGGAGTTCAACAAGGTCATCAAGTCGTTCCTAATCAATTTCAAGCGCTCTCAAATCGGTCCATCTGCTAATCCATAA

Protein Analysis

312

Amino Acids

35.38

Weight (kDa)

8.66

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 49 - 286 9.3e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 50 - 160 3.2e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 291 2.3e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016353)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37170
malus_domestica MD08G1183900.v1.1 MD15G1370400.v1.1
prunus_persica Prupe.1G518000_v2.0.a1
pyrus_communis pycom08g15770 pycom15g33210
rosa_chinensis RchiOBHm_Chr7g0238751
rosa_laevigata RLG00000000815
rosa_multiflora Rmu_sc0010914.1_g000009
rosa_roxburghii Rroxscaffold_3G00221580
rosa_rugosa Rorug07G0323400
rosa_samantha Rh7AG477700 Rh7CG495700 Rh7DG462700
rosa_wichuraiana Rw7G039870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 101, 526
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 449, 842
AcoI YGGCCR 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 337
AcyI GRCGYC 2 cut(s) 177, 536
AfaI GTAC 3 cut(s) 392, 410, 566
AfeI AGCGCT 1 cut(s) 908
AfiI CCNNNNNNNGG 4 cut(s) 42, 69, 412, 590
AgsI TTSAA 3 cut(s) 653, 871, 904
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 6 cut(s) 15, 372, 547, 556, 667, 814
AluI AGCT 6 cut(s) 15, 372, 547, 556, 667, 814
Alw26I GTCTC 3 cut(s) 87, 290, 623
Aor51HI AGCGCT 1 cut(s) 908
ApeKI GCWGC 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 697
AspLEI GCGC 2 cut(s) 446, 909
AsuC2I CCSGG 3 cut(s) 59, 347, 551
AsuHPI GGTGA 3 cut(s) 10, 211, 445
AvaII GGWCC 6 cut(s) 45, 72, 85, 121, 469, 920
BaeGI GKGCMC 1 cut(s) 840
BalI TGGCCA 1 cut(s) 677
BanI GGYRCC 2 cut(s) 101, 526
BauI CACGAG 1 cut(s) 359
BbvCI CCTCAGC 2 cut(s) 368, 720
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 686, 931
BceAI ACGGC 5 cut(s) 112, 184, 391, 571, 586
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 2 cut(s) 669, 822
BcnI CCSGG 3 cut(s) 59, 347, 551
BcoDI GTCTC 3 cut(s) 87, 290, 623
BfmI CTRYAG 1 cut(s) 473
BfoI RGCGCY 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 363
BisI GCNGC 9 cut(s) 100, 379, 402, 442, 447, 513, 530, 581, 584
BlpI GCTNAGC 1 cut(s) 810
BlsI GCNGC 9 cut(s) 101, 380, 403, 443, 448, 514, 531, 582, 585
Bme1390I CCNGG 4 cut(s) 59, 305, 347, 551
Bme18I GGWCC 6 cut(s) 45, 72, 85, 121, 469, 920
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 103, 122, 123, 528
BmrFI CCNGG 4 cut(s) 59, 305, 347, 551
BmrI ACTGGG 2 cut(s) 16, 400
BmsI GCATC 1 cut(s) 820
BmuI ACTGGG 2 cut(s) 16, 400
BplI GAGNNNNNCTC 2 cut(s) 323, 355
Bpu10I CCTNAGC 2 cut(s) 368, 720
Bpu1102I GCTNAGC 1 cut(s) 810
BpuMI CCSGG 3 cut(s) 59, 347, 551
BsaHI GRCGYC 2 cut(s) 177, 536
BsaJI CCNNGG 4 cut(s) 166, 172, 447, 765
BsaWI WCCGGW 2 cut(s) 42, 69
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 69, 412, 590
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 22, 406
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 4 cut(s) 166, 172, 447, 765
BseGI GGATG 4 cut(s) 54, 207, 678, 835
BseLI CCNNNNNNNGG 4 cut(s) 42, 69, 412, 590
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 5 cut(s) 103, 382, 628, 734, 824
BseNI ACTGG 2 cut(s) 22, 406
BseRI GAGGAG 6 cut(s) 146, 149, 152, 242, 259, 476
BseSI GKGCMC 1 cut(s) 840
BseXI GCAGC 1 cut(s) 428
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 449, 842
Bsh1285I CGRYCG 1 cut(s) 289
BshNI GGYRCC 2 cut(s) 101, 526
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 7 cut(s) 43, 58, 63, 70, 346, 539, 550
BslFI GGGAC 2 cut(s) 107, 227
BslI CCNNNNNNNGG 4 cut(s) 42, 69, 412, 590
BsmAI GTCTC 3 cut(s) 87, 290, 623
BsmBI CGTCTC 2 cut(s) 87, 290
BsmFI GGGAC 2 cut(s) 107, 227
BsmI GAATGC 1 cut(s) 811
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 2 cut(s) 669, 822
Bsp1720I GCTNAGC 1 cut(s) 810
BspCNI CTCAG 5 cut(s) 102, 381, 627, 733, 823
BspFNI CGCG 2 cut(s) 449, 842
BspLI GGNNCC 4 cut(s) 103, 122, 123, 528
BspMAI CTGCAG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 480
BspT107I GGYRCC 2 cut(s) 101, 526
BsrBI CCGCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 22, 406
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 4 cut(s) 166, 172, 447, 765
BssMI GATC 2 cut(s) 669, 822
BssNI GRCGYC 2 cut(s) 177, 536
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 2 cut(s) 177, 536
BstC8I GCNNGC 6 cut(s) 64, 399, 517, 611, 816, 840
BstDEI CTNAG 5 cut(s) 89, 368, 614, 720, 810
BstDSI CCRYGG 2 cut(s) 166, 447
BstF5I GGATG 4 cut(s) 54, 207, 678, 835
BstFNI CGCG 2 cut(s) 449, 842
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 2 cut(s) 446, 909
BstKTI GATC 2 cut(s) 672, 825
BstMAI GTCTC 3 cut(s) 87, 290, 623
BstMBI GATC 2 cut(s) 669, 822
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 8 cut(s) 363, 378, 535, 544, 553, 815, 839, 848
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 4 cut(s) 57, 303, 345, 549
BstSFI CTRYAG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 840
BstUI CGCG 2 cut(s) 449, 842
BstV1I GCAGC 1 cut(s) 428
BstXI CCANNNNNNTGG 1 cut(s) 523
BtgI CCRYGG 2 cut(s) 166, 447
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 4 cut(s) 54, 207, 678, 835
BtsI GCAGTG 2 cut(s) 119, 825
BtsIMutI CAGTG 2 cut(s) 119, 825
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 6 cut(s) 64, 399, 517, 611, 816, 840
CfoI GCGC 2 cut(s) 446, 909
Cfr10I RCCGGY 1 cut(s) 62
Cfr42I CCGCGG 1 cut(s) 450
CseI GACGC 3 cut(s) 166, 304, 544
Csp6I GTAC 3 cut(s) 391, 409, 565
CviAII CATG 6 cut(s) 6, 109, 319, 328, 673, 749
CviQI GTAC 3 cut(s) 391, 409, 565
DdeI CTNAG 5 cut(s) 89, 368, 614, 720, 810
DpnI GATC 2 cut(s) 671, 824
DpnII GATC 2 cut(s) 669, 822
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 765
Eco47I GGWCC 6 cut(s) 45, 72, 85, 121, 469, 920
Eco47III AGCGCT 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 337
EcoO109I RGGNCCY 2 cut(s) 121, 469
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 765
Esp3I CGTCTC 2 cut(s) 87, 290
FaeI CATG 6 cut(s) 9, 112, 322, 331, 676, 752
FaqI GGGAC 2 cut(s) 107, 227
FatI CATG 6 cut(s) 5, 108, 318, 327, 672, 748
FauI CCCGC 1 cut(s) 315
FbaI TGATCA 2 cut(s) 669, 822
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 9 cut(s) 100, 379, 402, 442, 447, 513, 530, 581, 584
FokI GGATG 4 cut(s) 41, 194, 665, 842
FseI GGCCGGCC 1 cut(s) 66
Fsp4HI GCNGC 9 cut(s) 100, 379, 402, 442, 447, 513, 530, 581, 584
GlaI GCGC 2 cut(s) 445, 908
GluI GCNGC 9 cut(s) 100, 379, 402, 442, 447, 513, 530, 581, 584
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 910
HapII CCGG 7 cut(s) 43, 58, 63, 70, 346, 539, 550
HgaI GACGC 3 cut(s) 166, 304, 544
HhaI GCGC 2 cut(s) 446, 909
Hin1I GRCGYC 2 cut(s) 177, 536
Hin1II CATG 6 cut(s) 9, 112, 322, 331, 676, 752
Hin6I GCGC 2 cut(s) 444, 907
HinP1I GCGC 2 cut(s) 444, 907
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 2 cut(s) 266, 715
HpaII CCGG 7 cut(s) 43, 58, 63, 70, 346, 539, 550
HphI GGTGA 3 cut(s) 10, 211, 445
Hpy166II GTNNAC 5 cut(s) 75, 181, 238, 567, 639
Hpy188I TCNGA 5 cut(s) 92, 295, 499, 617, 703
Hpy188III TCNNGA 2 cut(s) 628, 653
Hpy8I GTNNAC 5 cut(s) 75, 181, 238, 567, 639
Hpy99I CGWCG 2 cut(s) 389, 607
HpyAV CCTTC 3 cut(s) 361, 460, 716
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 4 cut(s) 216, 234, 240, 299
HpyCH4V TGCA 6 cut(s) 112, 475, 793, 818, 833, 861
HpyF10VI GCNNNNNNNGC 8 cut(s) 363, 378, 535, 544, 553, 815, 839, 848
HpyF3I CTNAG 5 cut(s) 89, 368, 614, 720, 810
HpySE526I ACGT 4 cut(s) 216, 234, 240, 299
Hsp92I GRCGYC 2 cut(s) 177, 536
Hsp92II CATG 6 cut(s) 9, 112, 322, 331, 676, 752
HspAI GCGC 2 cut(s) 444, 907
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 2 cut(s) 669, 822
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 2 cut(s) 669, 822
LmnI GCTCC 3 cut(s) 296, 552, 553
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 1 cut(s) 820
MaeII ACGT 4 cut(s) 216, 234, 240, 299
MaeIII GTNAC 2 cut(s) 79, 217
MalI GATC 2 cut(s) 671, 824
MbiI CCGCTC 1 cut(s) 291
MboI GATC 2 cut(s) 669, 822
MboII GAAGA 3 cut(s) 247, 250, 670
MhlI GDGCHC 1 cut(s) 840
MlsI TGGCCA 1 cut(s) 677
MluCI AATT 1 cut(s) 898
MluNI TGGCCA 1 cut(s) 677
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 72, 726
Mox20I TGGCCA 1 cut(s) 677
MroNI GCCGGC 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 697
MscI TGGCCA 1 cut(s) 677
MslI CAYNNNNRTG 3 cut(s) 107, 524, 753
Msp20I TGGCCA 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 7 cut(s) 43, 58, 63, 70, 346, 539, 550
MspR9I CCNGG 4 cut(s) 59, 305, 347, 551
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 2 cut(s) 449, 842
MwoI GCNNNNNNNGC 8 cut(s) 363, 378, 535, 544, 553, 815, 839, 848
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 3 cut(s) 59, 347, 551
NdeII GATC 2 cut(s) 669, 822
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 6 cut(s) 9, 112, 322, 331, 676, 752
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 528
NmeAIII GCCGAG 2 cut(s) 153, 334
NmuCI GTSAC 2 cut(s) 79, 217
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 381
PctI GAATGC 1 cut(s) 811
PdiI GCCGGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 697
PfeI GAWTC 1 cut(s) 715
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 9 cut(s) 101, 380, 403, 443, 448, 514, 531, 582, 585
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 2 cut(s) 121, 469
PsiI TTATAA 1 cut(s) 734
Psp5II RGGWCCY 2 cut(s) 121, 469
Psp6I CCWGG 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 528
PspPPI RGGWCCY 2 cut(s) 121, 469
PstI CTGCAG 1 cut(s) 477
RigI GGCCGGCC 1 cut(s) 66
RsaI GTAC 3 cut(s) 392, 410, 566
RsaNI GTAC 3 cut(s) 391, 409, 565
RseI CAYNNNNRTG 3 cut(s) 107, 524, 753
SacII CCGCGG 1 cut(s) 450
SatI GCNGC 9 cut(s) 100, 379, 402, 442, 447, 513, 530, 581, 584
Sau3AI GATC 2 cut(s) 669, 822
SbfI CCTGCAGG 1 cut(s) 477
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 4 cut(s) 59, 305, 347, 551
SdaI CCTGCAGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 1 cut(s) 820
SfcI CTRYAG 1 cut(s) 473
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 6 cut(s) 45, 72, 85, 121, 469, 920
SmiMI CAYNNNNRTG 3 cut(s) 107, 524, 753
Sse8387I CCTGCAGG 1 cut(s) 477
Sse9I AATT 1 cut(s) 898
StyD4I CCNGG 4 cut(s) 57, 303, 345, 549
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 4 cut(s) 219, 237, 243, 302
TaqI TCGA 1 cut(s) 627
TaqII GACCGA 1 cut(s) 908
TasI AATT 1 cut(s) 898
TatI WGTACW 2 cut(s) 408, 564
TauI GCSGC 8 cut(s) 102, 381, 404, 449, 515, 532, 583, 586
TfiI GAWTC 1 cut(s) 715
TscAI CASTG 2 cut(s) 119, 825
TseFI GTSAC 2 cut(s) 79, 217
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 79, 217
TspDTI ATGAA 2 cut(s) 43, 613
TspGWI ACGGA 2 cut(s) 98, 331
TspRI CASTG 2 cut(s) 119, 825
VpaK11BI GGWCC 6 cut(s) 45, 72, 85, 121, 469, 920
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.