Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0016353

Physical Location & Seq

Genomic Coordinates Forward (+)

45030195 .. 45033156

Gene Structure 2962 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1370400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGCCATGCTTCAGCATCACCCAGTCCAAAAATTCATGCCACCGGTTCACCTTCGCCCGAGCCGGCCTCCGGTCTGCAATCACCGACCTCAAAGACGGCGCCACCACCATGCACTGCTGGGTCCCGCAATCTCCGAACCCTTCCAAGCCCAACCTCCTCCTCATCCACGGCTTCGGCGTCAACGCAATGTGGCAGTTCGTTGACCTCCTCCGCCATGTCACCCCTCACTACAACGTCTACGTGCCGGATCTCGTCTTCTTCGGCGACTCCTACACGACCCGGCCCGACCGGTCCGAGTGGTTCCAGGCCGAGTGCGTGATGCAGGCGATGGAGGCGCACTCGGTGCGGAGGCTGAGCCTGGTGGGGCTGAGCTACGGCGGGTTTGTGGGCTACAGTTTGGCTGCCATGTACAAGGAGGCGGTGGAGAGGGTTGTGATATGCGGCGCCGCCGTGTGCCTGGAGGAGAAGGACCTCTGGGAAGGTGCGTTTCGGATCTCGGATTTGGATGAGGCGGCCGGGATTTTGACGCCGCAGACGCCTGAGAAGCTGAAGGAGTTGGTCAGGTACACGTTTTTCAAGCCACCTCCAGTTGGGTTGCTGCCCTCTTGCTTGCTCATGGATTTCATTCAGGCAATGTACTCCGAATTTGTGCAAGAGAAGAAAGAGTTGATCCAGGCCATCCCCAAAAACCGAAAACTTTCAGACCTCCCCAAGATTCCTCAGCCAACCCTTATAGTTTGGGGAGAACATGATCAAGTATTTCCAGTTGAATTTGCTCACAAATTAAAAAGGCACTTGGGAGAAAATGCTCAGCTTGTAGTAATCAAGGATGCAGGGCATGCATTGAACGCAGAAAAGACCAAGGAGTACCACAAGCACATCAAGTCCTTCTTACTCCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0006629 GO:0006638 GO:0006639 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0016020 GO:0016021 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0031224 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044425 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_4

Protein Analysis

301

Amino Acids

34.05

Weight (kDa)

8.11

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	49 - 286	1.9e-09	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	50 - 158	5.7e-14	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	52 - 285	4e-19	Alpha/beta hydrolase family
Abhydrolase_1	PF00561	222 - 286	1.9e-06	alpha/beta hydrolase fold
Abhydrolase_4	PF08386	238 - 299	8.7e-06	TAP-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016353)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g37170
malus_domestica	MD08G1183900.v1.1 MD15G1370400.v1.1
prunus_persica	Prupe.1G518000_v2.0.a1
pyrus_communis	pycom08g15770 pycom15g33210
rosa_chinensis	RchiOBHm_Chr7g0238751
rosa_laevigata	RLG00000000815
rosa_multiflora	Rmu_sc0010914.1_g000009
rosa_roxburghii	Rroxscaffold_3G00221580
rosa_rugosa	Rorug07G0323400
rosa_samantha	Rh7AG477700 Rh7CG495700 Rh7DG462700
rosa_wichuraiana	Rw7G039870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	98, 443
AccI	GTMKAC	1 cut(s)	237
AciI	CCGC	9 cut(s)	125, 211, 346, 378, 419, 441, 447, 512, 530
AclWI	GGATC	3 cut(s)	255, 500, 664
AcoI	YGGCCR	1 cut(s)	513
AcsI	RAATTY	3 cut(s)	31, 644, 770
AcuI	CTGAAG	1 cut(s)	569
AcyI	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
AdeI	CACNNNGTG	1 cut(s)	343
AfaI	GTAC	4 cut(s)	410, 566, 638, 869
AfiI	CCNNNNNNNGG	2 cut(s)	69, 590
AflIII	ACRYGT	1 cut(s)	567
AgeI	ACCGGT	2 cut(s)	42, 288
AgsI	TTSAA	3 cut(s)	577, 770, 847
AjnI	CCWGG	4 cut(s)	303, 357, 456, 672
AluBI	AGCT	3 cut(s)	372, 547, 814
AluI	AGCT	3 cut(s)	372, 547, 814
AlwI	GGATC	3 cut(s)	255, 500, 664
Ama87I	CYCGRG	1 cut(s)	57
AoxI	GGCC	5 cut(s)	64, 281, 306, 513, 675
ApeKI	GCWGC	2 cut(s)	401, 598
ApoI	RAATTY	3 cut(s)	31, 644, 770
AsiGI	ACCGGT	2 cut(s)	42, 288
Asp700I	GAANNNNTTC	1 cut(s)	697
AspLEI	GCGC	3 cut(s)	101, 337, 446
AspS9I	GGNCC	4 cut(s)	121, 282, 291, 469
AsuC2I	CCSGG	2 cut(s)	280, 517
AsuHPI	GGTGA	4 cut(s)	10, 40, 73, 211
AvaI	CYCGRG	1 cut(s)	57
AvaII	GGWCC	3 cut(s)	121, 291, 469
BanI	GGYRCC	2 cut(s)	98, 443
BbsI	GAAGAC	1 cut(s)	247
BbvCI	CCTCAGC	1 cut(s)	720
BbvI	GCAGC	2 cut(s)	388, 585
BccI	CCATC	2 cut(s)	322, 686
BceAI	ACGGC	4 cut(s)	112, 184, 391, 434
BciT130I	CCWGG	4 cut(s)	305, 359, 458, 674
BclI	TGATCA	1 cut(s)	751
BcnI	CCSGG	2 cut(s)	280, 517
BfmI	CTRYAG	1 cut(s)	391
BfoI	RGCGCY	2 cut(s)	102, 447
BisI	GCNGC	6 cut(s)	402, 442, 447, 513, 530, 599
BlpI	GCTNAGC	3 cut(s)	353, 368, 810
BlsI	GCNGC	6 cut(s)	403, 443, 448, 514, 531, 600
Bme1390I	CCNGG	6 cut(s)	280, 305, 359, 458, 517, 674
Bme18I	GGWCC	3 cut(s)	121, 291, 469
BmeT110I	CYCGRG	1 cut(s)	57
BmgT120I	GGNCC	4 cut(s)	121, 282, 291, 469
BmiI	GGNNCC	5 cut(s)	100, 122, 123, 302, 445
BmrFI	CCNGG	6 cut(s)	280, 305, 359, 458, 517, 674
BmrI	ACTGGG	1 cut(s)	16
BmsI	GCATC	3 cut(s)	24, 309, 820
BmuI	ACTGGG	1 cut(s)	16
BpiI	GAAGAC	1 cut(s)	247
BplI	GAGNNNNNCTC	4 cut(s)	51, 83, 323, 355
BpmI	CTGGAG	2 cut(s)	479, 570
Bpu10I	CCTNAGC	1 cut(s)	720
Bpu1102I	GCTNAGC	3 cut(s)	353, 368, 810
BpuMI	CCSGG	2 cut(s)	280, 517
BsaAI	YACGTR	1 cut(s)	241
BsaHI	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
BsaJI	CCNNGG	2 cut(s)	166, 861
BsaWI	WCCGGW	3 cut(s)	42, 69, 288
Bsc4I	CCNNNNNNNGG	2 cut(s)	69, 590
Bse118I	RCCGGY	3 cut(s)	42, 62, 288
Bse1I	ACTGG	3 cut(s)	22, 587, 764
Bse3DI	GCAATG	2 cut(s)	192, 639
BseBI	CCWGG	4 cut(s)	305, 359, 458, 674
BseDI	CCNNGG	2 cut(s)	166, 861
BseGI	GGATG	4 cut(s)	162, 511, 678, 835
BseLI	CCNNNNNNNGG	2 cut(s)	69, 590
BseMI	GCAATG	2 cut(s)	192, 639
BseMII	CTCAG	5 cut(s)	344, 359, 531, 734, 824
BseNI	ACTGG	3 cut(s)	22, 587, 764
BseRI	GAGGAG	4 cut(s)	146, 149, 197, 476
BseX3I	CGGCCG	1 cut(s)	513
BseXI	GCAGC	2 cut(s)	388, 585
BseYI	CCCAGC	1 cut(s)	117
Bsh1285I	CGRYCG	2 cut(s)	289, 516
BshFI	GGCC	5 cut(s)	66, 283, 308, 515, 677
BshNI	GGYRCC	2 cut(s)	98, 443
BshTI	ACCGGT	2 cut(s)	42, 288
BsiEI	CGRYCG	2 cut(s)	289, 516
BsiHKCI	CYCGRG	1 cut(s)	57
BsiSI	CCGG	7 cut(s)	43, 63, 70, 245, 280, 289, 516
BslFI	GGGAC	1 cut(s)	107
BslI	CCNNNNNNNGG	2 cut(s)	69, 590
BsmFI	GGGAC	1 cut(s)	107
BsnI	GGCC	5 cut(s)	66, 283, 308, 515, 677
BsoBI	CYCGRG	1 cut(s)	57
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	4 cut(s)	247, 492, 669, 751
Bsp1720I	GCTNAGC	3 cut(s)	353, 368, 810
BspACI	CCGC	9 cut(s)	125, 211, 346, 378, 419, 441, 447, 512, 530
BspANI	GGCC	5 cut(s)	66, 283, 308, 515, 677
BspCNI	CTCAG	5 cut(s)	345, 360, 532, 733, 823
BspLI	GGNNCC	5 cut(s)	100, 122, 123, 302, 445
BspPI	GGATC	3 cut(s)	255, 500, 664
BspT107I	GGYRCC	2 cut(s)	98, 443
BsrDI	GCAATG	2 cut(s)	192, 639
BsrFI	RCCGGY	3 cut(s)	42, 62, 288
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	3 cut(s)	22, 587, 764
BssAI	RCCGGY	3 cut(s)	42, 62, 288
BssECI	CCNNGG	2 cut(s)	166, 861
BssMI	GATC	4 cut(s)	247, 492, 669, 751
BssNI	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
BssT1I	CCWWGG	1 cut(s)	861
Bst2UI	CCWGG	4 cut(s)	305, 359, 458, 674
Bst4CI	ACNGT	1 cut(s)	395
BstACI	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
BstAPI	GCANNNNNTGC	2 cut(s)	343, 839
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	1 cut(s)	241
BstC8I	GCNNGC	4 cut(s)	64, 324, 611, 840
BstDEI	CTNAG	5 cut(s)	353, 368, 540, 720, 810
BstDSI	CCRYGG	1 cut(s)	166
BstF5I	GGATG	4 cut(s)	162, 511, 678, 835
BstH2I	RGCGCY	2 cut(s)	102, 447
BstHHI	GCGC	3 cut(s)	101, 337, 446
BstKTI	GATC	4 cut(s)	250, 495, 672, 754
BstMBI	GATC	4 cut(s)	247, 492, 669, 751
BstMCI	CGRYCG	2 cut(s)	289, 516
BstMWI	GCNNNNNNNGC	6 cut(s)	332, 343, 535, 544, 839, 848
BstNI	CCWGG	4 cut(s)	305, 359, 458, 674
BstNSI	RCATGY	1 cut(s)	842
BstSCI	CCNGG	6 cut(s)	278, 303, 357, 456, 515, 672
BstSFI	CTRYAG	1 cut(s)	391
BstV1I	GCAGC	2 cut(s)	388, 585
BstV2I	GAAGAC	1 cut(s)	247
BstX2I	RGATCY	2 cut(s)	247, 492
BstYI	RGATCY	2 cut(s)	247, 492
BstZI	CGGCCG	1 cut(s)	513
BsuRI	GGCC	5 cut(s)	66, 283, 308, 515, 677
BtgI	CCRYGG	1 cut(s)	166
BtgZI	GCGATG	1 cut(s)	341
BtsCI	GGATG	4 cut(s)	162, 511, 678, 835
BtsI	GCAGTG	1 cut(s)	112
BtsIMutI	CAGTG	1 cut(s)	112
Cac8I	GCNNGC	4 cut(s)	64, 324, 611, 840
CfoI	GCGC	3 cut(s)	101, 337, 446
Cfr10I	RCCGGY	3 cut(s)	42, 62, 288
Cfr13I	GGNCC	4 cut(s)	121, 282, 291, 469
CpoI	CGGWCCG	1 cut(s)	291
CseI	GACGC	3 cut(s)	166, 535, 544
Csp6I	GTAC	4 cut(s)	409, 565, 637, 868
CspAI	ACCGGT	2 cut(s)	42, 288
CspI	CGGWCCG	1 cut(s)	291
CviAII	CATG	8 cut(s)	6, 36, 109, 215, 406, 616, 749, 839
CviQI	GTAC	4 cut(s)	409, 565, 637, 868
DdeI	CTNAG	5 cut(s)	353, 368, 540, 720, 810
DinI	GGCGCC	2 cut(s)	100, 445
DpnI	GATC	4 cut(s)	249, 494, 671, 753
DpnII	GATC	4 cut(s)	247, 492, 669, 751
DraIII	CACNNNGTG	1 cut(s)	343
EaeI	YGGCCR	1 cut(s)	513
EagI	CGGCCG	1 cut(s)	513
EciI	GGCGGA	1 cut(s)	200
EclXI	CGGCCG	1 cut(s)	513
Eco130I	CCWWGG	1 cut(s)	861
Eco47I	GGWCC	3 cut(s)	121, 291, 469
Eco52I	CGGCCG	1 cut(s)	513
Eco57I	CTGAAG	1 cut(s)	569
Eco88I	CYCGRG	1 cut(s)	57
EcoO109I	RGGNCCY	2 cut(s)	121, 469
EcoRII	CCWGG	4 cut(s)	303, 357, 456, 672
EcoT14I	CCWWGG	1 cut(s)	861
EcoT22I	ATGCAT	1 cut(s)	844
EgeI	GGCGCC	2 cut(s)	100, 445
EheI	GGCGCC	2 cut(s)	100, 445
ErhI	CCWWGG	1 cut(s)	861
FaeI	CATG	8 cut(s)	9, 39, 112, 218, 409, 619, 752, 842
FalI	AAGNNNNNCTT	1 cut(s)	875
FaqI	GGGAC	1 cut(s)	107
FatI	CATG	8 cut(s)	5, 35, 108, 214, 405, 615, 748, 838
FauI	CCCGC	2 cut(s)	132, 371
FbaI	TGATCA	1 cut(s)	751
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	6 cut(s)	402, 442, 447, 513, 530, 599
FokI	GGATG	4 cut(s)	149, 518, 665, 842
Fsp4HI	GCNGC	6 cut(s)	402, 442, 447, 513, 530, 599
GlaI	GCGC	3 cut(s)	100, 336, 445
GluI	GCNGC	6 cut(s)	402, 442, 447, 513, 530, 599
GsaI	CCCAGC	1 cut(s)	121
GsuI	CTGGAG	2 cut(s)	479, 570
HaeII	RGCGCY	2 cut(s)	102, 447
HaeIII	GGCC	5 cut(s)	66, 283, 308, 515, 677
HapII	CCGG	7 cut(s)	43, 63, 70, 245, 280, 289, 516
HgaI	GACGC	3 cut(s)	166, 535, 544
HhaI	GCGC	3 cut(s)	101, 337, 446
Hin1I	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
Hin1II	CATG	8 cut(s)	9, 39, 112, 218, 409, 619, 752, 842
Hin6I	GCGC	3 cut(s)	99, 335, 444
HinP1I	GCGC	3 cut(s)	99, 335, 444
HincII	GTYRAC	2 cut(s)	181, 202
HindII	GTYRAC	2 cut(s)	181, 202
HinfI	GANTC	2 cut(s)	266, 715
HpaII	CCGG	7 cut(s)	43, 63, 70, 245, 280, 289, 516
HphI	GGTGA	4 cut(s)	10, 40, 73, 211
Hpy166II	GTNNAC	5 cut(s)	48, 181, 202, 238, 567
Hpy188I	TCNGA	7 cut(s)	135, 295, 492, 499, 643, 703, 899
Hpy8I	GTNNAC	5 cut(s)	48, 181, 202, 238, 567
HpyAV	CCTTC	6 cut(s)	61, 150, 460, 473, 544, 898
HpyCH4III	ACNGT	1 cut(s)	395
HpyCH4IV	ACGT	3 cut(s)	234, 240, 569
HpyCH4V	TGCA	6 cut(s)	77, 112, 322, 652, 833, 842
HpyF10VI	GCNNNNNNNGC	6 cut(s)	332, 343, 535, 544, 839, 848
HpyF3I	CTNAG	5 cut(s)	353, 368, 540, 720, 810
HpySE526I	ACGT	3 cut(s)	234, 240, 569
Hsp92I	GRCGYC	5 cut(s)	99, 177, 444, 527, 536
Hsp92II	CATG	8 cut(s)	9, 39, 112, 218, 409, 619, 752, 842
HspAI	GCGC	3 cut(s)	99, 335, 444
KasI	GGCGCC	2 cut(s)	98, 443
KflI	GGGWCCC	1 cut(s)	121
KroI	GCCGGC	1 cut(s)	62
KroNI	GCCGGC	1 cut(s)	64
Ksp22I	TGATCA	1 cut(s)	751
Kzo9I	GATC	4 cut(s)	247, 492, 669, 751
Lsp1109I	GCAGC	2 cut(s)	388, 585
LweI	GCATC	3 cut(s)	24, 309, 820
MaeII	ACGT	3 cut(s)	234, 240, 569
MaeIII	GTNAC	1 cut(s)	217
MalI	GATC	4 cut(s)	249, 494, 671, 753
MboI	GATC	4 cut(s)	247, 492, 669, 751
MboII	GAAGA	3 cut(s)	247, 250, 670
MflI	RGATCY	2 cut(s)	247, 492
MluCI	AATT	4 cut(s)	31, 644, 770, 782
Mly113I	GGCGCC	2 cut(s)	99, 444
MlyI	GAGTC	1 cut(s)	260
Mph1103I	ATGCAT	1 cut(s)	844
MroNI	GCCGGC	1 cut(s)	62
MroXI	GAANNNNTTC	1 cut(s)	697
MseI	TTAA	1 cut(s)	785
MslI	CAYNNNNRTG	1 cut(s)	107
MspI	CCGG	7 cut(s)	43, 63, 70, 245, 280, 289, 516
MspR9I	CCNGG	6 cut(s)	280, 305, 359, 458, 517, 674
MvaI	CCWGG	4 cut(s)	305, 359, 458, 674
MwoI	GCNNNNNNNGC	6 cut(s)	332, 343, 535, 544, 839, 848
NaeI	GCCGGC	1 cut(s)	64
NarI	GGCGCC	2 cut(s)	99, 444
NciI	CCSGG	2 cut(s)	280, 517
NdeII	GATC	4 cut(s)	247, 492, 669, 751
NgoMIV	GCCGGC	1 cut(s)	62
NlaIII	CATG	8 cut(s)	9, 39, 112, 218, 409, 619, 752, 842
NlaIV	GGNNCC	5 cut(s)	100, 122, 123, 302, 445
NmeAIII	GCCGAG	1 cut(s)	334
NmuCI	GTSAC	1 cut(s)	217
NsiI	ATGCAT	1 cut(s)	844
NspI	RCATGY	1 cut(s)	842
PaeI	GCATGC	1 cut(s)	842
PcsI	WCGNNNNNNNCGW	1 cut(s)	174
PdiI	GCCGGC	1 cut(s)	64
PdmI	GAANNNNTTC	1 cut(s)	697
PfeI	GAWTC	1 cut(s)	715
PinAI	ACCGGT	2 cut(s)	42, 288
PkrI	GCNGC	6 cut(s)	403, 443, 448, 514, 531, 600
PleI	GAGTC	1 cut(s)	260
PluTI	GGCGCC	2 cut(s)	102, 447
PpsI	GAGTC	1 cut(s)	260
Ppu21I	YACGTR	1 cut(s)	241
PpuMI	RGGWCCY	2 cut(s)	121, 469
Psp5II	RGGWCCY	2 cut(s)	121, 469
Psp6I	CCWGG	4 cut(s)	303, 357, 456, 672
PspFI	CCCAGC	1 cut(s)	117
PspGI	CCWGG	4 cut(s)	303, 357, 456, 672
PspN4I	GGNNCC	5 cut(s)	100, 122, 123, 302, 445
PspPI	GGNCC	4 cut(s)	121, 282, 291, 469
PspPPI	RGGWCCY	2 cut(s)	121, 469
PsuI	RGATCY	2 cut(s)	247, 492
RsaI	GTAC	4 cut(s)	410, 566, 638, 869
RsaNI	GTAC	4 cut(s)	409, 565, 637, 868
RseI	CAYNNNNRTG	1 cut(s)	107
Rsr2I	CGGWCCG	1 cut(s)	291
RsrII	CGGWCCG	1 cut(s)	291
SaqAI	TTAA	1 cut(s)	785
SatI	GCNGC	6 cut(s)	402, 442, 447, 513, 530, 599
Sau3AI	GATC	4 cut(s)	247, 492, 669, 751
Sau96I	GGNCC	4 cut(s)	121, 282, 291, 469
SchI	GAGTC	1 cut(s)	260
ScrFI	CCNGG	6 cut(s)	280, 305, 359, 458, 517, 674
SfaNI	GCATC	3 cut(s)	24, 309, 820
SfcI	CTRYAG	1 cut(s)	391
SfoI	GGCGCC	2 cut(s)	100, 445
SinI	GGWCC	3 cut(s)	121, 291, 469
SmiMI	CAYNNNNRTG	1 cut(s)	107
SphI	GCATGC	1 cut(s)	842
Sse9I	AATT	4 cut(s)	31, 644, 770, 782
SsiI	CCGC	9 cut(s)	125, 211, 346, 378, 419, 441, 447, 512, 530
SspDI	GGCGCC	2 cut(s)	98, 443
StyD4I	CCNGG	6 cut(s)	278, 303, 357, 456, 515, 672
StyI	CCWWGG	1 cut(s)	861
TaaI	ACNGT	1 cut(s)	395
TaiI	ACGT	3 cut(s)	237, 243, 572
TasI	AATT	4 cut(s)	31, 644, 770, 782
TatI	WGTACW	2 cut(s)	408, 636
TauI	GCSGC	4 cut(s)	444, 449, 515, 532
TfiI	GAWTC	1 cut(s)	715
Tru1I	TTAA	1 cut(s)	785
Tru9I	TTAA	1 cut(s)	785
TscAI	CASTG	1 cut(s)	119
TseFI	GTSAC	1 cut(s)	217
TseI	GCWGC	2 cut(s)	401, 598
Tsp45I	GTSAC	1 cut(s)	217
TspDTI	ATGAA	2 cut(s)	24, 613
TspRI	CASTG	1 cut(s)	119
VpaK11BI	GGWCC	3 cut(s)	121, 291, 469
XapI	RAATTY	3 cut(s)	31, 644, 770
XceI	RCATGY	1 cut(s)	842
XmiI	GTMKAC	1 cut(s)	237
XmnI	GAANNNNTTC	1 cut(s)	697
Zsp2I	ATGCAT	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.