Rmu_sc0010914.1_g000009

Monoacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010914.1
OG0016353
Physical Location & Seq
Forward (+)
26896 .. 29123
2228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010914.1_g000009.1.cds

Sequence Viewer

Length: 939 bp
atgccatgcttcagcttaacccagtcaaagaactcctgcttgcggtccaacttcatccgggccggcctccggcccaccgtcacggacctcaaagacggcggcaccaccatgcactgctgggtccccaaatccctcaacccctccaaacccaacctcctcctcctccacggcttcggcgtcaacgcaatgtggcaattcgctgacctcatccgccacgtcacccctcactacaacgtctacgtccccgacctcgtctttttcggcgactcctccaccacccgacccgaccgctccgagactttccaggccgagtgcgtcatgcgggtcatggaggcccactccgtccgggccttcagcctcgtgggcctcagctacggcgggttcgtcgggtacaggctggcggcccagtacaaggaggccgtcgagagggtggtgatttgctgcgccgcggtgtgtatggaggagaaggacctgcaggaaggagtgtttagggtttcggatttggacgaggccgccagcattttgatgccgcagacgccgggcaagctccgggagcttgtcaagtacacttttttcaggccgccgccgttggggtttatgccgtcgtgcttgctcagagatttcatcgagacaatgttcacagattttgttcaagaaaagaaagagctgatcatggccatccctaaaaaccgaaacctttcagaccttcccaagattcctcagccgactcttataatctggggagagcatgataatgtatttcccttggaatatgcccacaaactaaaaatgcacttgggggagaatgctcagcttgcagtaatcaaggatgcagggcacgcgataaacgctgaaaagtgcaaggagttcaacaaggtcatcaagtcgttcctaatcaatttcaagcgctcacaaatcggtccatctgctaatccataa

Protein Analysis

312

Amino Acids

35.2

Weight (kDa)

8.51

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016353)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37170
malus_domestica MD08G1183900.v1.1 MD15G1370400.v1.1
prunus_persica Prupe.1G518000_v2.0.a1
pyrus_communis pycom08g15770 pycom15g33210
rosa_chinensis RchiOBHm_Chr7g0238751
rosa_laevigata RLG00000000815
rosa_multiflora Rmu_sc0010914.1_g000009
rosa_roxburghii Rroxscaffold_3G00221580
rosa_rugosa Rorug07G0323400
rosa_samantha Rh7AG477700 Rh7CG495700 Rh7DG462700
rosa_wichuraiana Rw7G039870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 101
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 449, 842
AcoI YGGCCR 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 337
AcyI GRCGYC 2 cut(s) 177, 536
AfaI GTAC 3 cut(s) 392, 410, 566
AfeI AGCGCT 1 cut(s) 908
AfiI CCNNNNNNNGG 5 cut(s) 42, 69, 412, 426, 590
AgsI TTSAA 3 cut(s) 653, 871, 904
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 6 cut(s) 15, 372, 547, 556, 667, 814
AluI AGCT 6 cut(s) 15, 372, 547, 556, 667, 814
Alw26I GTCTC 2 cut(s) 290, 623
Aor51HI AGCGCT 1 cut(s) 908
ApeKI GCWGC 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 697
AspLEI GCGC 2 cut(s) 446, 909
AsuC2I CCSGG 4 cut(s) 59, 347, 540, 551
AsuHPI GGTGA 2 cut(s) 211, 445
AvaII GGWCC 5 cut(s) 45, 85, 121, 469, 920
BaeGI GKGCMC 1 cut(s) 840
BalI TGGCCA 1 cut(s) 677
BanI GGYRCC 1 cut(s) 101
BauI CACGAG 1 cut(s) 359
BbvCI CCTCAGC 2 cut(s) 368, 720
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 686, 931
BceAI ACGGC 6 cut(s) 112, 184, 391, 404, 571, 586
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 4 cut(s) 59, 347, 540, 551
BcoDI GTCTC 2 cut(s) 290, 623
BfmI CTRYAG 1 cut(s) 473
BfoI RGCGCY 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 363
BisI GCNGC 8 cut(s) 100, 402, 442, 447, 513, 530, 581, 584
BlpI GCTNAGC 1 cut(s) 810
BlsI GCNGC 8 cut(s) 101, 403, 443, 448, 514, 531, 582, 585
Bme1390I CCNGG 5 cut(s) 59, 305, 347, 540, 551
Bme18I GGWCC 5 cut(s) 45, 85, 121, 469, 920
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 3 cut(s) 103, 122, 123
BmrFI CCNGG 5 cut(s) 59, 305, 347, 540, 551
BmrI ACTGGG 2 cut(s) 16, 400
BmsI GCATC 2 cut(s) 516, 820
BmuI ACTGGG 2 cut(s) 16, 400
BplI GAGNNNNNCTC 2 cut(s) 323, 355
Bpu10I CCTNAGC 2 cut(s) 368, 720
Bpu1102I GCTNAGC 1 cut(s) 810
BpuMI CCSGG 4 cut(s) 59, 347, 540, 551
BsaHI GRCGYC 2 cut(s) 177, 536
BsaJI CCNNGG 3 cut(s) 166, 447, 765
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 69, 412, 426, 590
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 22, 406
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 166, 447, 765
BseGI GGATG 4 cut(s) 54, 207, 678, 835
BseLI CCNNNNNNNGG 5 cut(s) 42, 69, 412, 426, 590
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 4 cut(s) 382, 628, 734, 824
BseNI ACTGG 2 cut(s) 22, 406
BseRI GAGGAG 5 cut(s) 146, 149, 152, 259, 476
BseSI GKGCMC 1 cut(s) 840
BseXI GCAGC 1 cut(s) 428
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 449, 842
Bsh1285I CGRYCG 1 cut(s) 289
BshNI GGYRCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 6 cut(s) 58, 63, 70, 346, 539, 550
BslFI GGGAC 2 cut(s) 107, 227
BslI CCNNNNNNNGG 5 cut(s) 42, 69, 412, 426, 590
BsmAI GTCTC 2 cut(s) 290, 623
BsmFI GGGAC 2 cut(s) 107, 227
BsmI GAATGC 1 cut(s) 811
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 1 cut(s) 669
Bsp1720I GCTNAGC 1 cut(s) 810
BspCNI CTCAG 4 cut(s) 381, 627, 733, 823
BspFNI CGCG 2 cut(s) 449, 842
BspLI GGNNCC 3 cut(s) 103, 122, 123
BspMAI CTGCAG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 22, 406
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 166, 447, 765
BssMI GATC 1 cut(s) 669
BssNI GRCGYC 2 cut(s) 177, 536
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 2 cut(s) 177, 536
BstC8I GCNNGC 8 cut(s) 41, 64, 399, 517, 545, 611, 816, 840
BstDEI CTNAG 4 cut(s) 368, 614, 720, 810
BstDSI CCRYGG 2 cut(s) 166, 447
BstF5I GGATG 4 cut(s) 54, 207, 678, 835
BstFNI CGCG 2 cut(s) 449, 842
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 2 cut(s) 446, 909
BstKTI GATC 1 cut(s) 672
BstMAI GTCTC 2 cut(s) 290, 623
BstMBI GATC 1 cut(s) 669
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 7 cut(s) 363, 535, 544, 553, 815, 839, 848
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 5 cut(s) 57, 303, 345, 538, 549
BstSFI CTRYAG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 840
BstUI CGCG 2 cut(s) 449, 842
BstV1I GCAGC 1 cut(s) 428
BtgI CCRYGG 2 cut(s) 166, 447
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 4 cut(s) 54, 207, 678, 835
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 112
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 8 cut(s) 41, 64, 399, 517, 545, 611, 816, 840
CfoI GCGC 2 cut(s) 446, 909
Cfr10I RCCGGY 1 cut(s) 62
Cfr42I CCGCGG 1 cut(s) 450
CseI GACGC 3 cut(s) 166, 304, 544
Csp6I GTAC 3 cut(s) 391, 409, 565
CviAII CATG 6 cut(s) 6, 109, 319, 328, 673, 749
CviQI GTAC 3 cut(s) 391, 409, 565
DdeI CTNAG 4 cut(s) 368, 614, 720, 810
DpnI GATC 1 cut(s) 671
DpnII GATC 1 cut(s) 669
EaeI YGGCCR 1 cut(s) 675
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 765
Eco47I GGWCC 5 cut(s) 45, 85, 121, 469, 920
Eco47III AGCGCT 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 337
EcoO109I RGGNCCY 2 cut(s) 121, 469
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 6 cut(s) 9, 112, 322, 331, 676, 752
FaqI GGGAC 2 cut(s) 107, 227
FatI CATG 6 cut(s) 5, 108, 318, 327, 672, 748
FauI CCCGC 2 cut(s) 315, 371
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 8 cut(s) 100, 402, 442, 447, 513, 530, 581, 584
FokI GGATG 4 cut(s) 41, 194, 665, 842
FseI GGCCGGCC 1 cut(s) 66
Fsp4HI GCNGC 8 cut(s) 100, 402, 442, 447, 513, 530, 581, 584
GlaI GCGC 2 cut(s) 445, 908
GluI GCNGC 8 cut(s) 100, 402, 442, 447, 513, 530, 581, 584
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 910
HapII CCGG 6 cut(s) 58, 63, 70, 346, 539, 550
HgaI GACGC 3 cut(s) 166, 304, 544
HhaI GCGC 2 cut(s) 446, 909
Hin1I GRCGYC 2 cut(s) 177, 536
Hin1II CATG 6 cut(s) 9, 112, 322, 331, 676, 752
Hin6I GCGC 2 cut(s) 444, 907
HinP1I GCGC 2 cut(s) 444, 907
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 3 cut(s) 266, 715, 727
HpaII CCGG 6 cut(s) 58, 63, 70, 346, 539, 550
HphI GGTGA 2 cut(s) 211, 445
Hpy166II GTNNAC 4 cut(s) 181, 238, 567, 639
Hpy188I TCNGA 4 cut(s) 295, 499, 617, 703
Hpy188III TCNNGA 3 cut(s) 424, 628, 653
Hpy8I GTNNAC 4 cut(s) 181, 238, 567, 639
Hpy99I CGWCG 3 cut(s) 389, 425, 607
HpyAV CCTTC 4 cut(s) 361, 460, 473, 716
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 3 cut(s) 216, 234, 240
HpyCH4V TGCA 6 cut(s) 112, 475, 793, 818, 833, 861
HpyF10VI GCNNNNNNNGC 7 cut(s) 363, 535, 544, 553, 815, 839, 848
HpyF3I CTNAG 4 cut(s) 368, 614, 720, 810
HpySE526I ACGT 3 cut(s) 216, 234, 240
Hsp92I GRCGYC 2 cut(s) 177, 536
Hsp92II CATG 6 cut(s) 9, 112, 322, 331, 676, 752
HspAI GCGC 2 cut(s) 444, 907
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 669
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 1 cut(s) 669
LmnI GCTCC 3 cut(s) 296, 552, 553
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 2 cut(s) 516, 820
MaeII ACGT 3 cut(s) 216, 234, 240
MaeIII GTNAC 2 cut(s) 79, 217
MalI GATC 1 cut(s) 671
MbiI CCGCTC 1 cut(s) 291
MboI GATC 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 840
MlsI TGGCCA 1 cut(s) 677
MluCI AATT 2 cut(s) 194, 898
MluNI TGGCCA 1 cut(s) 677
MlyI GAGTC 2 cut(s) 260, 721
MmeI TCCRAC 1 cut(s) 72
Mox20I TGGCCA 1 cut(s) 677
MroNI GCCGGC 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 697
MscI TGGCCA 1 cut(s) 677
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 3 cut(s) 107, 524, 753
Msp20I TGGCCA 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 6 cut(s) 58, 63, 70, 346, 539, 550
MspR9I CCNGG 5 cut(s) 59, 305, 347, 540, 551
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 2 cut(s) 449, 842
MwoI GCNNNNNNNGC 7 cut(s) 363, 535, 544, 553, 815, 839, 848
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 4 cut(s) 59, 347, 540, 551
NdeII GATC 1 cut(s) 669
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 6 cut(s) 9, 112, 322, 331, 676, 752
NlaIV GGNNCC 3 cut(s) 103, 122, 123
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 2 cut(s) 79, 217
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 381
PctI GAATGC 1 cut(s) 811
PdiI GCCGGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 697
PfeI GAWTC 1 cut(s) 715
PflFI GACNNNGTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 8 cut(s) 101, 403, 443, 448, 514, 531, 582, 585
PleI GAGTC 2 cut(s) 260, 721
PpsI GAGTC 2 cut(s) 260, 721
PpuMI RGGWCCY 2 cut(s) 121, 469
PsiI TTATAA 1 cut(s) 734
Psp5II RGGWCCY 2 cut(s) 121, 469
Psp6I CCWGG 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 3 cut(s) 103, 122, 123
PspPPI RGGWCCY 2 cut(s) 121, 469
PstI CTGCAG 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 251
RigI GGCCGGCC 1 cut(s) 66
RsaI GTAC 3 cut(s) 392, 410, 566
RsaNI GTAC 3 cut(s) 391, 409, 565
RseI CAYNNNNRTG 3 cut(s) 107, 524, 753
SacII CCGCGG 1 cut(s) 450
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 8 cut(s) 100, 402, 442, 447, 513, 530, 581, 584
Sau3AI GATC 1 cut(s) 669
SbfI CCTGCAGG 1 cut(s) 477
SchI GAGTC 2 cut(s) 260, 721
ScrFI CCNGG 5 cut(s) 59, 305, 347, 540, 551
SdaI CCTGCAGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 2 cut(s) 516, 820
SfcI CTRYAG 1 cut(s) 473
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 5 cut(s) 45, 85, 121, 469, 920
SmiMI CAYNNNNRTG 3 cut(s) 107, 524, 753
Sse8387I CCTGCAGG 1 cut(s) 477
Sse9I AATT 2 cut(s) 194, 898
StyD4I CCNGG 5 cut(s) 57, 303, 345, 538, 549
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 3 cut(s) 219, 237, 243
TaqI TCGA 2 cut(s) 423, 627
TaqII GACCGA 1 cut(s) 908
TasI AATT 2 cut(s) 194, 898
TatI WGTACW 2 cut(s) 408, 564
TauI GCSGC 7 cut(s) 102, 404, 449, 515, 532, 583, 586
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 2 cut(s) 79, 217
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 79, 217
TspDTI ATGAA 2 cut(s) 43, 613
TspGWI ACGGA 2 cut(s) 98, 331
TspRI CASTG 1 cut(s) 119
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 5 cut(s) 45, 85, 121, 469, 920
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.