MD08G1194800.v1.1

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0019276
Physical Location & Seq
Reverse (-)
25055867 .. 25057972
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1194800.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGCTGCCTACGGCATGATCACCGCCTTCCAAGCGTCTTGGCTAAGATCCTCTTACTCCGGAGCCCATCTATGCAGACTGCCGCCATCATCATCATCATCATCAGTTGGGTTTCTCTCTCTGTCTTCACCAGCATCATCAGCTGGTAAAGTTTCGCAGAGCCGGCGCGTGTTGTCTTCGGAGCAGAAGGCCGCCGGAGTAGGAGGTCTGACGTACAAGGATGCTGGTGTAGATATAGATGCTGGAACGGCGCTTGTTGATAAAATTAAAGCCATGGCTCCTGAAATTGGATCCTTTGGGGGTATTGTCCATGATGAGATACTCCGCGAAAAAGACGTATGCCTTGTTCACGGGACGGATGGCGTTGGAACTAAACTTAAGCTTGCTTTTGAGACTGGAATTCATGACACCATTGGTATTGATTTGGTTGCCATGAATGTCAATGATATCGTTACTTGTGGGGCGAAGCCATTATCTTTCCAGGATTACTTTTCTACAAGCCGCCTTGATGTTGATATCGCTGTCAAGGTTATACAAGGTATTCGCGATGGTTGCCAACAATCTGGCTGCAGCTGTAAACTTTCAGGCGGAGAGACTGCAGAGATGCCGGATTTTTACAAAGACGGCGAGTATGACCTGGGCGGCTCTGCTGTTGGAGCTGTGAAAAAAAGGATTCGATCATTGATGGGAAAAACATTGCTCCCGGAGATGTCATCATTGGCCTGCCGTCCAGTGGGGGTTCTTGCTGATACTGGGGTTTCTCTCAAGGACCAACTGCCTGGTGGAGAAGGTGTCCTTGACTTAATAAGCAAGGGAGGTGTCAACGGAGTAGCTCATATCACAGGGGGTGGTTTCACAGAAAATATACCCCCGAGTGTTCCCGAAAGGCCTAGGAGCGGTCATTTATAA

Protein Analysis

303

Amino Acids

31.29

Weight (kDa)

6.08

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIRS PF00586 111 - 221 1.1e-20 AIR synthase related protein, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 907
AccBSI CCGCTC 1 cut(s) 897
AccII CGCG 3 cut(s) 168, 327, 546
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 8 cut(s) 24, 83, 192, 325, 502, 588, 642, 897
AclWI GGATC 3 cut(s) 42, 285, 298
AcsI RAATTY 1 cut(s) 399
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 3 cut(s) 287, 733, 896
AflII CTTAAG 1 cut(s) 377
AjnI CCWGG 3 cut(s) 480, 636, 778
AluBI AGCT 5 cut(s) 143, 382, 573, 659, 833
AluI AGCT 5 cut(s) 143, 382, 573, 659, 833
Alw26I GTCTC 2 cut(s) 386, 587
AlwI GGATC 3 cut(s) 42, 285, 298
Ama87I CYCGRG 1 cut(s) 871
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 3 cut(s) 189, 720, 887
ApeKI GCWGC 3 cut(s) 5, 567, 570
ApoI RAATTY 1 cut(s) 399
AspA2I CCTAGG 1 cut(s) 890
AspLEI GCGC 2 cut(s) 168, 253
AspS9I GGNCC 1 cut(s) 769
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 2 cut(s) 13, 120
AvaI CYCGRG 1 cut(s) 871
AvaII GGWCC 1 cut(s) 769
AvrII CCTAGG 1 cut(s) 890
BaeI ACNNNNGTAYC 2 cut(s) 741, 774
BamHI GGATCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 67
BbsI GAAGAC 2 cut(s) 117, 168
BbvI GCAGC 2 cut(s) 554, 582
BccI CCATC 5 cut(s) 75, 94, 353, 542, 679
BceAI ACGGC 4 cut(s) 28, 264, 640, 711
BciT130I CCWGG 3 cut(s) 482, 638, 780
BclI TGATCA 1 cut(s) 18
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 2 cut(s) 386, 587
BfaI CTAG 1 cut(s) 891
BfmI CTRYAG 2 cut(s) 568, 597
BfoI RGCGCY 1 cut(s) 254
BfrI CTTAAG 1 cut(s) 377
BisI GCNGC 7 cut(s) 6, 83, 192, 502, 568, 571, 643
BlnI CCTAGG 1 cut(s) 890
BlsI GCNGC 7 cut(s) 7, 84, 193, 503, 569, 572, 644
Bme1390I CCNGG 4 cut(s) 482, 638, 704, 780
Bme18I GGWCC 1 cut(s) 769
BmeT110I CYCGRG 1 cut(s) 871
BmgT120I GGNCC 1 cut(s) 769
BmiI GGNNCC 3 cut(s) 64, 279, 292
BmrFI CCNGG 4 cut(s) 482, 638, 704, 780
BmrI ACTGGG 1 cut(s) 762
BmsI GCATC 4 cut(s) 143, 211, 229, 594
BmuI ACTGGG 1 cut(s) 762
BpiI GAAGAC 2 cut(s) 117, 168
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 273, 637, 890
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 733, 896
Bse118I RCCGGY 1 cut(s) 162
Bse1I ACTGG 3 cut(s) 400, 731, 757
Bse3DI GCAATG 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 59
BseBI CCWGG 3 cut(s) 482, 638, 780
BseDI CCNNGG 3 cut(s) 273, 637, 890
BseGI GGATG 2 cut(s) 226, 364
BseLI CCNNNNNNNGG 3 cut(s) 287, 733, 896
BseMI GCAATG 1 cut(s) 695
BseNI ACTGG 3 cut(s) 400, 731, 757
BseXI GCAGC 2 cut(s) 554, 582
Bsh1236I CGCG 3 cut(s) 168, 327, 546
BshFI GGCC 3 cut(s) 191, 722, 889
BsiHKCI CYCGRG 1 cut(s) 871
BsiSI CCGG 5 cut(s) 60, 163, 195, 608, 704
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 287, 733, 896
BsmAI GTCTC 2 cut(s) 386, 587
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 3 cut(s) 191, 722, 889
BsoBI CYCGRG 1 cut(s) 871
Bsp1286I GDGCHC 1 cut(s) 67
Bsp13I TCCGGA 1 cut(s) 59
Bsp143I GATC 4 cut(s) 18, 47, 290, 677
Bsp19I CCATGG 1 cut(s) 273
Bsp68I TCGCGA 1 cut(s) 546
BspACI CCGC 8 cut(s) 24, 83, 192, 325, 502, 588, 642, 897
BspANI GGCC 3 cut(s) 191, 722, 889
BspEI TCCGGA 1 cut(s) 59
BspFNI CGCG 3 cut(s) 168, 327, 546
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 64, 279, 292
BspMAI CTGCAG 2 cut(s) 572, 601
BspPI GGATC 3 cut(s) 42, 285, 298
BspTI CTTAAG 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 897
BsrDI GCAATG 1 cut(s) 695
BsrFI RCCGGY 1 cut(s) 162
BsrI ACTGG 3 cut(s) 400, 731, 757
BssAI RCCGGY 1 cut(s) 162
BssECI CCNNGG 3 cut(s) 273, 637, 890
BssMI GATC 4 cut(s) 18, 47, 290, 677
BssT1I CCWWGG 2 cut(s) 273, 890
Bst2UI CCWGG 3 cut(s) 482, 638, 780
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 164, 384, 724
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 2 cut(s) 226, 364
BstFNI CGCG 3 cut(s) 168, 327, 546
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 168, 253
BstKTI GATC 4 cut(s) 21, 50, 293, 680
BstMAI GTCTC 2 cut(s) 386, 587
BstMBI GATC 4 cut(s) 18, 47, 290, 677
BstMWI GCNNNNNNNGC 6 cut(s) 32, 140, 163, 248, 552, 656
BstNI CCWGG 3 cut(s) 482, 638, 780
BstSCI CCNGG 4 cut(s) 480, 636, 702, 778
BstSFI CTRYAG 2 cut(s) 568, 597
BstUI CGCG 3 cut(s) 168, 327, 546
BstV1I GCAGC 2 cut(s) 554, 582
BstV2I GAAGAC 2 cut(s) 117, 168
BstX2I RGATCY 2 cut(s) 47, 290
BstXI CCANNNNNNTGG 2 cut(s) 563, 779
BstYI RGATCY 2 cut(s) 47, 290
BsuRI GGCC 3 cut(s) 191, 722, 889
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 2 cut(s) 226, 364
BtsIMutI CAGTG 1 cut(s) 738
BtuMI TCGCGA 1 cut(s) 546
Cac8I GCNNGC 3 cut(s) 164, 384, 724
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 168, 253
Cfr10I RCCGGY 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 769
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 214
CviAII CATG 5 cut(s) 16, 274, 311, 404, 433
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 44
DpnI GATC 4 cut(s) 20, 49, 292, 679
DpnII GATC 4 cut(s) 18, 47, 290, 677
EciI GGCGGA 1 cut(s) 603
Eco130I CCWWGG 2 cut(s) 273, 890
Eco147I AGGCCT 1 cut(s) 889
Eco24I GRGCYC 1 cut(s) 67
Eco32I GATATC 2 cut(s) 448, 517
Eco47I GGWCC 1 cut(s) 769
Eco88I CYCGRG 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 399
EcoRII CCWGG 3 cut(s) 480, 636, 778
EcoRV GATATC 2 cut(s) 448, 517
EcoT14I CCWWGG 2 cut(s) 273, 890
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 273, 890
FaeI CATG 5 cut(s) 19, 277, 314, 407, 436
FalI AAGNNNNNCTT 4 cut(s) 37, 69, 780, 812
FaqI GGGAC 1 cut(s) 367
FatI CATG 5 cut(s) 15, 273, 310, 403, 432
FbaI TGATCA 1 cut(s) 18
Fnu4HI GCNGC 7 cut(s) 6, 83, 192, 502, 568, 571, 643
FokI GGATG 2 cut(s) 233, 371
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 7 cut(s) 6, 83, 192, 502, 568, 571, 643
FspBI CTAG 1 cut(s) 891
GlaI GCGC 2 cut(s) 167, 252
GluI GCNGC 7 cut(s) 6, 83, 192, 502, 568, 571, 643
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 3 cut(s) 191, 722, 889
HapII CCGG 5 cut(s) 60, 163, 195, 608, 704
HgaI GACGC 1 cut(s) 24
HhaI GCGC 2 cut(s) 168, 253
Hin1II CATG 5 cut(s) 19, 277, 314, 407, 436
Hin6I GCGC 2 cut(s) 166, 251
HinP1I GCGC 2 cut(s) 166, 251
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 673
HpaII CCGG 5 cut(s) 60, 163, 195, 608, 704
HphI GGTGA 2 cut(s) 13, 120
Hpy166II GTNNAC 3 cut(s) 349, 578, 823
Hpy188I TCNGA 2 cut(s) 181, 210
Hpy188III TCNNGA 5 cut(s) 60, 281, 404, 545, 881
Hpy8I GTNNAC 3 cut(s) 349, 578, 823
HpyAV CCTTC 3 cut(s) 37, 181, 782
HpyCH4IV ACGT 2 cut(s) 212, 336
HpyCH4V TGCA 3 cut(s) 75, 570, 599
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 140, 163, 248, 552, 656
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 2 cut(s) 212, 336
Hsp92II CATG 5 cut(s) 19, 277, 314, 407, 436
HspAI GCGC 2 cut(s) 166, 251
Kpn2I TCCGGA 1 cut(s) 59
KroI GCCGGC 1 cut(s) 162
KroNI GCCGGC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 4 cut(s) 18, 47, 290, 677
LmnI GCTCC 6 cut(s) 62, 181, 283, 656, 705, 894
Lsp1109I GCAGC 2 cut(s) 554, 582
LweI GCATC 4 cut(s) 143, 211, 229, 594
MaeI CTAG 1 cut(s) 891
MaeII ACGT 2 cut(s) 212, 336
MaeIII GTNAC 1 cut(s) 451
MalI GATC 4 cut(s) 20, 49, 292, 679
MbiI CCGCTC 1 cut(s) 897
MboI GATC 4 cut(s) 18, 47, 290, 677
MboII GAAGA 2 cut(s) 117, 168
MflI RGATCY 2 cut(s) 47, 290
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 264, 285, 399
MmeI TCCRAC 2 cut(s) 346, 634
MnlI CCTC 3 cut(s) 61, 197, 809
MroI TCCGGA 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 162
MseI TTAA 3 cut(s) 267, 378, 803
MspA1I CMGCKG 2 cut(s) 143, 573
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 5 cut(s) 60, 163, 195, 608, 704
MspR9I CCNGG 4 cut(s) 482, 638, 704, 780
MvaI CCWGG 3 cut(s) 482, 638, 780
MvnI CGCG 3 cut(s) 168, 327, 546
MwoI GCNNNNNNNGC 6 cut(s) 32, 140, 163, 248, 552, 656
NaeI GCCGGC 1 cut(s) 164
NciI CCSGG 1 cut(s) 704
NcoI CCATGG 1 cut(s) 273
NdeII GATC 4 cut(s) 18, 47, 290, 677
NgoMIV GCCGGC 1 cut(s) 162
NlaIII CATG 5 cut(s) 19, 277, 314, 407, 436
NlaIV GGNNCC 3 cut(s) 64, 279, 292
NruI TCGCGA 1 cut(s) 546
PagI TCATGA 1 cut(s) 403
PceI AGGCCT 1 cut(s) 889
PdiI GCCGGC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 673
PfoI TCCNGGA 2 cut(s) 480, 702
PkrI GCNGC 7 cut(s) 7, 84, 193, 503, 569, 572, 644
PsiI TTATAA 1 cut(s) 907
Psp6I CCWGG 3 cut(s) 480, 636, 778
PspGI CCWGG 3 cut(s) 480, 636, 778
PspN4I GGNNCC 3 cut(s) 64, 279, 292
PspPI GGNCC 1 cut(s) 769
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
PstI CTGCAG 2 cut(s) 572, 601
PsuI RGATCY 2 cut(s) 47, 290
PvuII CAGCTG 2 cut(s) 143, 573
RruI TCGCGA 1 cut(s) 546
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 267, 378, 803
SatI GCNGC 7 cut(s) 6, 83, 192, 502, 568, 571, 643
Sau3AI GATC 4 cut(s) 18, 47, 290, 677
Sau96I GGNCC 1 cut(s) 769
ScrFI CCNGG 4 cut(s) 482, 638, 704, 780
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 4 cut(s) 143, 211, 229, 594
SfcI CTRYAG 2 cut(s) 568, 597
SinI GGWCC 1 cut(s) 769
SmlI CTYRAG 2 cut(s) 377, 764
SmoI CTYRAG 2 cut(s) 377, 764
Sse9I AATT 3 cut(s) 264, 285, 399
SseBI AGGCCT 1 cut(s) 889
SsiI CCGC 8 cut(s) 24, 83, 192, 325, 502, 588, 642, 897
SspMI CTAG 1 cut(s) 891
StuI AGGCCT 1 cut(s) 889
StyD4I CCNGG 4 cut(s) 480, 636, 702, 778
StyI CCWWGG 2 cut(s) 273, 890
TaiI ACGT 2 cut(s) 215, 339
TaqI TCGA 1 cut(s) 676
TasI AATT 3 cut(s) 264, 285, 399
TauI GCSGC 4 cut(s) 85, 194, 504, 645
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 3 cut(s) 267, 378, 803
Tru9I TTAA 3 cut(s) 267, 378, 803
TscAI CASTG 1 cut(s) 738
TseI GCWGC 3 cut(s) 5, 567, 570
TspDTI ATGAA 2 cut(s) 392, 449
TspGWI ACGGA 2 cut(s) 371, 840
TspRI CASTG 1 cut(s) 738
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 1 cut(s) 399
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XmaJI CCTAGG 1 cut(s) 890
XspI CTAG 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.