MD08G1195000.v1.1

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0019276
Physical Location & Seq
Forward (+)
25090421 .. 25092360
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1195000.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCTCCTGAAATTGGATCCTTTGGGGGTATTGTCCATGATGAGATACTCCGCGAAAAAGACGTATGCCTTGTTCACGGGACGGATGGCGTTGGAACTAAACTTAAGCTTGCTTTTGAGACTGGAATTCATGACACCATTGGTATTGATTTGGTAATCTACCACCTCCGCTTCTTGTATACATATTATTTTCTATTGATAATTGCTTTTCCAATGCCCCGACTATATTCGTTCTATCGTTCGTTCAACTCTCAGGTTGCCATGAATGTCAATGATATCGTTACTTGTGGGGCGAAGCCATTATCTTTCCAGGATTACTTTTCTACAAGCCGCCTTGATGTTGATATCGCTGTCAAGACTGCAGAGATGCCGGATTTTTACAAAGACGGCGAGTATGACCTGGGCGGCTCTGCTGTTGGAGCTGTGAAAAAGGATTCGATCATTGATGGGAAAAACATTGCTCCCGGAGATGTCATCATTGGCCTGCCGTCCAGTGGGGTTCATTCCAATGGTTTTTCTCTTGTGAGAAGGGTTCTTGCTGATACTGGGGTTTCTCTCAAGGACCAACTGCCTGGTGGAGAAGGTGTTACATTTGGCGAAGCTTTGATGGTCCCGACTGTGATATATGTTAATCAGCAAGGGAGGGGTGTCAACGGAGTAGCTCATATCACAGGGGGTGGTTTCACAGAAAATATACCCCGAGTGTTCCCGAAAGGCCTAGGAGCGGTCATTTATAAAGACTCATGGGAAGTCCCACCAGTTTTCAAATGGATCCAGAAGGCCGGAAGAGTAGAAGAGGCTGAGATGAGACGGACTTTTAACATGGGAATTGGCATGGTTCTTGTTGTGAGTCGGAGGCATCCCACAGGATACTTGAAGAGGATGGGAATGGAGCTGCTTATAAAGTATACCGCATCGGTGAGGTTGTAA

Protein Analysis

310

Amino Acids

33.89

Weight (kDa)

7.06

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIRS_C PF02769 155 - 284 1.8e-28 AIR synthase related protein, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 735, 902
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 2 cut(s) 179, 908
AccII CGCG 1 cut(s) 54
AciI CCGC 6 cut(s) 52, 169, 331, 405, 725, 912
AclWI GGATC 4 cut(s) 12, 25, 766, 779
AcsI RAATTY 1 cut(s) 126
AfiI CCNNNNNNNGG 3 cut(s) 14, 494, 724
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 3 cut(s) 247, 766, 877
AjnI CCWGG 3 cut(s) 309, 399, 571
AluBI AGCT 5 cut(s) 109, 422, 602, 662, 895
AluI AGCT 5 cut(s) 109, 422, 602, 662, 895
Alw26I GTCTC 2 cut(s) 113, 802
AlwI GGATC 4 cut(s) 12, 25, 766, 779
Ama87I CYCGRG 1 cut(s) 699
AoxI GGCC 3 cut(s) 481, 715, 780
ApeKI GCWGC 1 cut(s) 895
ApoI RAATTY 1 cut(s) 126
AspA2I CCTAGG 1 cut(s) 718
AspS9I GGNCC 2 cut(s) 562, 610
AsuC2I CCSGG 1 cut(s) 465
AvaI CYCGRG 1 cut(s) 699
AvaII GGWCC 2 cut(s) 562, 610
AvrII CCTAGG 1 cut(s) 718
BaeI ACNNNNGTAYC 2 cut(s) 534, 567
BamHI GGATCC 2 cut(s) 17, 771
BbvI GCAGC 1 cut(s) 882
BccI CCATC 4 cut(s) 80, 440, 601, 877
BceAI ACGGC 2 cut(s) 403, 472
BciT130I CCWGG 3 cut(s) 311, 401, 573
BciVI GTATCC 1 cut(s) 863
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 2 cut(s) 113, 802
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 104
BfuI GTATCC 1 cut(s) 863
BisI GCNGC 3 cut(s) 331, 406, 896
BlnI CCTAGG 1 cut(s) 718
BlsI GCNGC 3 cut(s) 332, 407, 897
Bme1390I CCNGG 4 cut(s) 311, 401, 465, 573
Bme18I GGWCC 2 cut(s) 562, 610
BmeT110I CYCGRG 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 562, 610
BmiI GGNNCC 4 cut(s) 6, 19, 612, 773
BmrFI CCNGG 4 cut(s) 311, 401, 465, 573
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 3 cut(s) 357, 868, 923
BmuI ACTGGG 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 400, 718
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 494, 724
Bse1I ACTGG 4 cut(s) 127, 492, 550, 758
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 3 cut(s) 311, 401, 573
BseDI CCNNGG 2 cut(s) 400, 718
BseGI GGATG 3 cut(s) 91, 859, 888
BseLI CCNNNNNNNGG 3 cut(s) 14, 494, 724
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 266, 792
BseNI ACTGG 4 cut(s) 127, 492, 550, 758
BseXI GCAGC 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 483, 717, 782
BsiHKCI CYCGRG 1 cut(s) 699
BsiSI CCGG 3 cut(s) 371, 465, 783
BslFI GGGAC 3 cut(s) 94, 596, 737
BslI CCNNNNNNNGG 3 cut(s) 14, 494, 724
BsmAI GTCTC 2 cut(s) 113, 802
BsmBI CGTCTC 1 cut(s) 802
BsmFI GGGAC 3 cut(s) 94, 596, 737
BsnI GGCC 3 cut(s) 483, 717, 782
BsoBI CYCGRG 1 cut(s) 699
Bsp143I GATC 3 cut(s) 17, 438, 771
BspACI CCGC 6 cut(s) 52, 169, 331, 405, 725, 912
BspANI GGCC 3 cut(s) 483, 717, 782
BspCNI CTCAG 2 cut(s) 265, 793
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 4 cut(s) 6, 19, 612, 773
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 4 cut(s) 12, 25, 766, 779
BspTI CTTAAG 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 4 cut(s) 127, 492, 550, 758
BssECI CCNNGG 2 cut(s) 400, 718
BssMI GATC 3 cut(s) 17, 438, 771
BssNAI GTATAC 2 cut(s) 180, 909
BssT1I CCWWGG 1 cut(s) 718
Bst1107I GTATAC 2 cut(s) 180, 909
Bst2UI CCWGG 3 cut(s) 311, 401, 573
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 3 cut(s) 781, 789, 872
BstAFI CTTAAG 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 111, 485
BstDEI CTNAG 2 cut(s) 252, 801
BstF5I GGATG 3 cut(s) 91, 859, 888
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 3 cut(s) 20, 441, 774
BstMAI GTCTC 2 cut(s) 113, 802
BstMBI GATC 3 cut(s) 17, 438, 771
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 3 cut(s) 311, 401, 573
BstSCI CCNGG 4 cut(s) 309, 399, 463, 571
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 882
BstX2I RGATCY 2 cut(s) 17, 771
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 2 cut(s) 17, 771
BstZ17I GTATAC 2 cut(s) 180, 909
BsuI GTATCC 1 cut(s) 863
BsuRI GGCC 3 cut(s) 483, 717, 782
BtsCI GGATG 3 cut(s) 91, 859, 888
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 111, 485
CciI TCATGA 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 562, 610
CviAII CATG 6 cut(s) 38, 131, 262, 744, 823, 835
DdeI CTNAG 2 cut(s) 252, 801
DpnI GATC 3 cut(s) 19, 440, 773
DpnII GATC 3 cut(s) 17, 438, 771
Eam1104I CTCTTC 3 cut(s) 781, 789, 872
EarI CTCTTC 3 cut(s) 781, 789, 872
Eco130I CCWWGG 1 cut(s) 718
Eco147I AGGCCT 1 cut(s) 717
Eco32I GATATC 2 cut(s) 277, 346
Eco47I GGWCC 2 cut(s) 562, 610
Eco88I CYCGRG 1 cut(s) 699
EcoRI GAATTC 1 cut(s) 126
EcoRII CCWGG 3 cut(s) 309, 399, 571
EcoRV GATATC 2 cut(s) 277, 346
EcoT14I CCWWGG 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 718
Esp3I CGTCTC 1 cut(s) 802
FaeI CATG 6 cut(s) 41, 134, 265, 747, 826, 838
FaqI GGGAC 3 cut(s) 94, 596, 737
FatI CATG 6 cut(s) 37, 130, 261, 743, 822, 834
FblI GTMKAC 2 cut(s) 179, 908
Fnu4HI GCNGC 3 cut(s) 331, 406, 896
FokI GGATG 3 cut(s) 98, 846, 895
Fsp4HI GCNGC 3 cut(s) 331, 406, 896
FspBI CTAG 1 cut(s) 719
GluI GCNGC 3 cut(s) 331, 406, 896
HaeIII GGCC 3 cut(s) 483, 717, 782
HapII CCGG 3 cut(s) 371, 465, 783
Hin1II CATG 6 cut(s) 41, 134, 265, 747, 826, 838
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 2 cut(s) 107, 600
HinfI GANTC 3 cut(s) 434, 740, 850
HpaII CCGG 3 cut(s) 371, 465, 783
Hpy166II GTNNAC 4 cut(s) 76, 180, 652, 909
Hpy188I TCNGA 1 cut(s) 855
Hpy188III TCNNGA 6 cut(s) 8, 131, 355, 613, 709, 775
Hpy8I GTNNAC 4 cut(s) 76, 180, 652, 909
HpyAV CCTTC 3 cut(s) 522, 575, 772
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 2 cut(s) 252, 801
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 41, 134, 265, 747, 826, 838
Kzo9I GATC 3 cut(s) 17, 438, 771
LmnI GCTCC 5 cut(s) 10, 419, 466, 722, 892
Lsp1109I GCAGC 1 cut(s) 882
LweI GCATC 3 cut(s) 357, 868, 923
MaeI CTAG 1 cut(s) 719
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 2 cut(s) 280, 586
MalI GATC 3 cut(s) 19, 440, 773
MbiI CCGCTC 1 cut(s) 725
MboI GATC 3 cut(s) 17, 438, 771
MboII GAAGA 3 cut(s) 798, 806, 889
MflI RGATCY 2 cut(s) 17, 771
MluCI AATT 4 cut(s) 12, 126, 201, 828
MlyI GAGTC 2 cut(s) 734, 859
MmeI TCCRAC 3 cut(s) 73, 397, 833
MnlI CCTC 6 cut(s) 176, 636, 790, 849, 873, 915
MseI TTAA 3 cut(s) 105, 630, 819
MslI CAYNNNNRTG 1 cut(s) 507
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 3 cut(s) 371, 465, 783
MspR9I CCNGG 4 cut(s) 311, 401, 465, 573
MvaI CCWGG 3 cut(s) 311, 401, 573
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 419
NciI CCSGG 1 cut(s) 465
NdeII GATC 3 cut(s) 17, 438, 771
NlaIII CATG 6 cut(s) 41, 134, 265, 747, 826, 838
NlaIV GGNNCC 4 cut(s) 6, 19, 612, 773
PagI TCATGA 1 cut(s) 130
PceI AGGCCT 1 cut(s) 717
PfeI GAWTC 1 cut(s) 434
PfoI TCCNGGA 2 cut(s) 309, 463
PkrI GCNGC 3 cut(s) 332, 407, 897
PleI GAGTC 2 cut(s) 734, 858
PpsI GAGTC 2 cut(s) 734, 858
PsiI TTATAA 2 cut(s) 735, 902
Psp6I CCWGG 3 cut(s) 309, 399, 571
PspGI CCWGG 3 cut(s) 309, 399, 571
PspN4I GGNNCC 4 cut(s) 6, 19, 612, 773
PspPI GGNCC 2 cut(s) 562, 610
PsrI GAACNNNNNNTAC 2 cut(s) 57, 89
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 2 cut(s) 17, 771
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 3 cut(s) 105, 630, 819
SatI GCNGC 3 cut(s) 331, 406, 896
Sau3AI GATC 3 cut(s) 17, 438, 771
Sau96I GGNCC 2 cut(s) 562, 610
SchI GAGTC 2 cut(s) 734, 859
ScrFI CCNGG 4 cut(s) 311, 401, 465, 573
SfaNI GCATC 3 cut(s) 357, 868, 923
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 2 cut(s) 562, 610
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 104, 557
SmoI CTYRAG 2 cut(s) 104, 557
Sse9I AATT 4 cut(s) 12, 126, 201, 828
SseBI AGGCCT 1 cut(s) 717
SsiI CCGC 6 cut(s) 52, 169, 331, 405, 725, 912
SspMI CTAG 1 cut(s) 719
StuI AGGCCT 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 309, 399, 463, 571
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 437
TasI AATT 4 cut(s) 12, 126, 201, 828
TauI GCSGC 2 cut(s) 333, 408
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 3 cut(s) 105, 630, 819
Tru9I TTAA 3 cut(s) 105, 630, 819
TscAI CASTG 1 cut(s) 499
TseI GCWGC 1 cut(s) 895
TspDTI ATGAA 3 cut(s) 119, 278, 491
TspGWI ACGGA 3 cut(s) 98, 669, 826
TspRI CASTG 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 2 cut(s) 562, 610
XapI RAATTY 1 cut(s) 126
XcmI CCANNNNNNNNNTGG 2 cut(s) 572, 765
XmaJI CCTAGG 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 179, 908
XspI CTAG 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.