Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015293

Physical Location & Seq

Genomic Coordinates Reverse (-)

28197142 .. 28205361

Gene Structure 8220 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1219300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1896 bp

ATGGAGGACATAGAGGATTTGTTGATCGGGAGCGGCGGCGGAGCTCCTCCCGGTTTTCGTCTGCCGATAAACACCGTGGGATTGAACCCAAAGAAGAAACCCAATCCCAATGGCCGCAATACCAACGCCAAGCTATCTCAAATCCAAGACCCACTCGCTCCTCTCTCTCCCAAAGTCCCCGGCACTCAGACTATATATATCAAAACATTTGGATGCTCACACAACCAGAGTGACAGTGAATATATGGCTGGTCAACTTTCAGCTTTCGGGTATCCATTAAGTGACAATCCTGAGGAGGCAGACCTGTGGCTCATAAATACATGCACGGTCAAATCTCCAAGTCAGTCTGCCATGGACACTCTGATAGCAAAAGGTAAAGGTGCAAGCAAGCCCCTGGTGGTGGCTGGGTGTGTGCCACAAGGAAGTCGAGATCTAAAGGAGCTAGAAGGTGTCAGTATTGTAGGAGTCCAGCAAATTGATCGTGTGGTTGAAATCGTTGAAGAGACTTTGAAAGGTCATGAGGTGCGCCTTTTGAGCCGTAAGACATTGCCTGCACTTGACCTCCCTAAGGTGAGGAAAAACAAGTTCGTTGAGATTCTTCCCATAAATGTTGGTTGTTTAGGTGCTTGTACTTACTGCAAGACAAAGCATGCTCGTGGTCATTTAGGAAGTTATACTGTTGATAGCCTTGTGGGACGTGTAAAATCTGTGGTAGCTGATGGAGTTAAGGAGATATGGTTGAGCAGTGAAGATACTGGTGCTTATGGTCGTGACATTGGGGTCAATCTACCAATTTTATTGAATTCAATTGTCGCAGAACTCCCTCCTGGTGCAAGGACAATGTTACGGATAGGGATGACGAATCCTCCATTCATTCTGGAGCACTTGAAGGAAATAGCAGAGGTTTTGCGTCATCCATGTGTATACTCATTTCTACATGTACCAGTCCAGTCTGGAAGTGATACAGTCTTGACTGCAATGAATCGGGAATATACTGTAAGTGAGTTCAAGACTGTGGTTGATACCCTAACTGAGCTTGTGCCAGGGATGCAGATTGCGACTGATATAATATGTGGATTTCCTGGTGAAACGGATGAAGATTTTACTCAAACTCTTAGCCTTATTAAAGAGTACAAGTTCTCTCAAGTTCATATTTCGCAGTTTTATCCTAGACCAGGAACACCTGCTGCAAGGATGAAAAAGGTCCCTAGTACTGTAGTGAAGAAAAGGAGTCGTGAATTGACTTCTGCTTTTGAAGCTTTCGCACCATATGTTGGGATGGAGGGCAGGGTGGAAAGAATATGGATAACTGATATTGCGACTGATGGAACTCACTTGGTTGGCCATACAAAGGGATACGTGCAGGTTCTTGTAGCCGCTCCAGAAAGTATGTTGGGGACTTCAGCAATTGTGAAGATAACATCGGTGGGAAGGTGGTCAGTTTTTGGAGAAGTGATTGAGACCATCCCTCACATAAATGATAAACCCACCTCAACAAATGAAAACCGTTATCAGGAGAAATGCTTCCCTGGTGCCAACAGTTGCGAGACTTGCGCGTGTTCAACTTCACCAGAAACTCGTGCCTGTGGACCAGAAAGCTGTGGAGGACAGGCTACCTCGGAAGAATGTGCTGTAACAAAGAATGCCGTTCTGCTGGAATGCTGGAACAGTAGAAATCCGATTGGATGGTTGCTGAGGAAGCGAAAAAACCATGTTGAGAAACAGGTGGAGGAGGAAATTGGCTTGCGATCCCAGAACAAGCAAGAGCAAGGCCAAGGACGTTTGAGTGATTGGGGTTTCGTCGATAGGGCTCTTCTAGGCGGGATGCTCGTGAGCTTCGTGACAATTGTAGCGCTACTAGTACATCTTGGCTTCAGGATTATGTCGTCTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0012505 GO:0016070 GO:0016740 GO:0016782 GO:0034470 GO:0034641 GO:0034660 GO:0035596 GO:0035598 GO:0035600 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050497 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Radical_SAM TRAM UPF0004

Protein Analysis

632

Amino Acids

68.76

Weight (kDa)

6.89

Isoelectric Point (pI)

45.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UPF0004	PF00919	65 - 143	2.7e-20	Uncharacterized protein family UPF0004
Radical_SAM	PF04055	202 - 375	2.3e-20	Radical SAM superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72090
fragaria_vesca	FvH4_5g32030
malus_domestica	MD08G1219300.v1.1 MD15G1407900.v1.1
prunus_persica	Prupe.1G553700_v2.0.a1
pyrus_communis	pycom08g19030 pycom15g36400
rosa_chinensis	RchiOBHm_Chr7g0228031
rosa_laevigata	RLG00000001594
rosa_multiflora	Rmu_sc0004858.1_g000015
rosa_roxburghii	Rroxscaffold_3G00231640
rosa_rugosa	Rorug07G0245400
rosa_samantha	Rh7AG399200 Rh7CG417300 Rh7DG394700
rosa_wichuraiana	Rw7G033180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1192
AasI	GACNNNNNNGTC	1 cut(s)	779
Acc36I	ACCTGC	2 cut(s)	1192, 1354
AccB1I	GGYRCC	1 cut(s)	1532
AccB7I	CCANNNNNTGG	1 cut(s)	1274
AccBSI	CCGCTC	2 cut(s)	33, 1379
AccI	GTMKAC	1 cut(s)	924
AccII	CGCG	1 cut(s)	1556
AciI	CCGC	6 cut(s)	33, 36, 39, 115, 1377, 1821
AclWI	GGATC	1 cut(s)	1743
AcoI	YGGCCR	2 cut(s)	112, 1342
AcsI	RAATTY	1 cut(s)	802
AcuI	CTGAAG	2 cut(s)	1386, 1858
AfaI	GTAC	5 cut(s)	631, 942, 1133, 1213, 1863
AfeI	AGCGCT	1 cut(s)	1854
AfiI	CCNNNNNNNGG	6 cut(s)	400, 568, 1175, 1274, 1351, 1513
AflIII	ACRYGT	2 cut(s)	697, 937
AhlI	ACTAGT	1 cut(s)	1858
AjiI	CACGTC	1 cut(s)	698
AjnI	CCWGG	6 cut(s)	393, 826, 1042, 1081, 1174, 1528
AluBI	AGCT	9 cut(s)	44, 133, 263, 442, 716, 1036, 1259, 1599, 1836
AluI	AGCT	9 cut(s)	44, 133, 263, 442, 716, 1036, 1259, 1599, 1836
Alw21I	GWGCWC	2 cut(s)	46, 885
Alw26I	GTCTC	3 cut(s)	497, 1454, 1541
AlwI	GGATC	1 cut(s)	1743
Aor51HI	AGCGCT	1 cut(s)	1854
AoxI	GGCC	3 cut(s)	112, 1342, 1771
ApeKI	GCWGC	1 cut(s)	1187
ApoI	RAATTY	1 cut(s)	802
Asp700I	GAANNNNTTC	1 cut(s)	1523
AspLEI	GCGC	3 cut(s)	528, 1556, 1855
AspS9I	GGNCC	2 cut(s)	1204, 1589
AsuC2I	CCSGG	2 cut(s)	51, 180
AsuHPI	GGTGA	3 cut(s)	583, 1097, 1560
AvaII	GGWCC	2 cut(s)	1204, 1589
AxyI	CCTNAGG	2 cut(s)	291, 567
BalI	TGGCCA	1 cut(s)	1344
BanI	GGYRCC	1 cut(s)	1532
BanII	GRGCYC	2 cut(s)	46, 1813
BauI	CACGAG	3 cut(s)	654, 1578, 1829
Bbv12I	GWGCWC	2 cut(s)	46, 885
BbvCI	CCTCAGC	1 cut(s)	1694
BbvI	GCAGC	1 cut(s)	1174
BccI	CCATC	5 cut(s)	713, 1273, 1319, 1472, 1680
BceAI	ACGGC	2 cut(s)	522, 1631
BcgI	CGANNNNNNTGC	2 cut(s)	461, 495
BciT130I	CCWGG	6 cut(s)	395, 828, 1044, 1083, 1176, 1530
BciVI	GTATCC	2 cut(s)	282, 1349
BcnI	CCSGG	2 cut(s)	51, 180
BcoDI	GTCTC	3 cut(s)	497, 1454, 1541
BcuI	ACTAGT	1 cut(s)	1858
BfaI	CTAG	5 cut(s)	443, 1170, 1209, 1817, 1859
BfmI	CTRYAG	1 cut(s)	1215
BfoI	RGCGCY	1 cut(s)	1856
BfuAI	ACCTGC	2 cut(s)	1192, 1354
BfuI	GTATCC	2 cut(s)	282, 1349
BglII	AGATCT	1 cut(s)	430
BisI	GCNGC	5 cut(s)	34, 37, 115, 1188, 1377
BlsI	GCNGC	5 cut(s)	35, 38, 116, 1189, 1378
BmcAI	AGTACT	1 cut(s)	1213
Bme1390I	CCNGG	8 cut(s)	51, 180, 395, 828, 1044, 1083, 1176, 1530
Bme18I	GGWCC	2 cut(s)	1204, 1589
BmgBI	CACGTC	1 cut(s)	698
BmgT120I	GGNCC	2 cut(s)	1204, 1589
BmiI	GGNNCC	2 cut(s)	1206, 1534
BmrFI	CCNGG	8 cut(s)	51, 180, 395, 828, 1044, 1083, 1176, 1530
BmsI	GCATC	3 cut(s)	203, 1038, 1815
BpmI	CTGGAG	2 cut(s)	899, 1365
Bpu10I	CCTNAGC	1 cut(s)	1694
BpuEI	CTTGAG	1 cut(s)	1128
BpuMI	CCSGG	2 cut(s)	51, 180
BsaAI	YACGTR	1 cut(s)	1360
BsaI	GGTCTC	1 cut(s)	1454
BsaJI	CCNNGG	8 cut(s)	75, 178, 351, 393, 1043, 1528, 1617, 1774
BsaXI	ACNNNNNCTCC	6 cut(s)	546, 576, 722, 752, 850, 880
Bsc4I	CCNNNNNNNGG	6 cut(s)	400, 568, 1175, 1274, 1351, 1513
Bse1I	ACTGG	3 cut(s)	760, 944, 949
Bse21I	CCTNAGG	2 cut(s)	291, 567
Bse3DI	GCAATG	2 cut(s)	545, 984
BseBI	CCWGG	6 cut(s)	395, 828, 1044, 1083, 1176, 1530
BseDI	CCNNGG	8 cut(s)	75, 178, 351, 393, 1043, 1528, 1617, 1774
BseLI	CCNNNNNNNGG	6 cut(s)	400, 568, 1175, 1274, 1351, 1513
BseMI	GCAATG	2 cut(s)	545, 984
BseMII	CTCAG	4 cut(s)	200, 282, 1023, 1685
BseNI	ACTGG	3 cut(s)	760, 944, 949
BseRI	GAGGAG	4 cut(s)	36, 150, 308, 1745
BseXI	GCAGC	1 cut(s)	1174
BseYI	CCCAGC	1 cut(s)	404
BsgI	GTGCAG	2 cut(s)	537, 1382
Bsh1236I	CGCG	1 cut(s)	1556
BshFI	GGCC	3 cut(s)	114, 1344, 1773
BshNI	GGYRCC	1 cut(s)	1532
BsiHKAI	GWGCWC	2 cut(s)	46, 885
BsiSI	CCGG	2 cut(s)	51, 180
BslFI	GGGAC	4 cut(s)	161, 708, 1190, 1411
BslI	CCNNNNNNNGG	6 cut(s)	400, 568, 1175, 1274, 1351, 1513
BsmAI	GTCTC	3 cut(s)	497, 1454, 1541
BsmFI	GGGAC	4 cut(s)	161, 708, 1190, 1411
BsmI	GAATGC	2 cut(s)	1648, 1664
BsnI	GGCC	3 cut(s)	114, 1344, 1773
Bso31I	GGTCTC	1 cut(s)	1454
Bsp1286I	GDGCHC	3 cut(s)	46, 885, 1813
Bsp143I	GATC	4 cut(s)	24, 430, 478, 1748
Bsp19I	CCATGG	1 cut(s)	351
BspACI	CCGC	6 cut(s)	33, 36, 39, 115, 1377, 1821
BspANI	GGCC	3 cut(s)	114, 1344, 1773
BspCNI	CTCAG	4 cut(s)	199, 283, 1024, 1686
BspFNI	CGCG	1 cut(s)	1556
BspHI	TCATGA	1 cut(s)	517
BspLI	GGNNCC	2 cut(s)	1206, 1534
BspMI	ACCTGC	2 cut(s)	1192, 1354
BspPI	GGATC	1 cut(s)	1743
BspQI	GCTCTTC	1 cut(s)	1818
BspT107I	GGYRCC	1 cut(s)	1532
BspTNI	GGTCTC	1 cut(s)	1454
BsrBI	CCGCTC	2 cut(s)	33, 1379
BsrDI	GCAATG	2 cut(s)	545, 984
BsrI	ACTGG	3 cut(s)	760, 944, 949
BssECI	CCNNGG	8 cut(s)	75, 178, 351, 393, 1043, 1528, 1617, 1774
BssMI	GATC	4 cut(s)	24, 430, 478, 1748
BssNAI	GTATAC	1 cut(s)	925
BssSI	CACGAG	3 cut(s)	654, 1578, 1829
BssT1I	CCWWGG	2 cut(s)	351, 1774
Bst1107I	GTATAC	1 cut(s)	925
Bst2BI	CACGAG	3 cut(s)	654, 1578, 1829
Bst2UI	CCWGG	6 cut(s)	395, 828, 1044, 1083, 1176, 1530
Bst6I	CTCTTC	2 cut(s)	495, 1818
BstBAI	YACGTR	1 cut(s)	1360
BstC8I	GCNNGC	5 cut(s)	385, 389, 552, 651, 1745
BstDEI	CTNAG	6 cut(s)	186, 291, 567, 1032, 1115, 1694
BstDSI	CCRYGG	2 cut(s)	75, 351
BstENI	CCTNNNNNAGG	2 cut(s)	566, 1173
BstFNI	CGCG	1 cut(s)	1556
BstH2I	RGCGCY	1 cut(s)	1856
BstHHI	GCGC	3 cut(s)	528, 1556, 1855
BstKTI	GATC	4 cut(s)	27, 433, 481, 1751
BstMAI	GTCTC	3 cut(s)	497, 1454, 1541
BstMBI	GATC	4 cut(s)	24, 430, 478, 1748
BstMWI	GCNNNNNNNGC	5 cut(s)	534, 1048, 1256, 1551, 1699
BstNI	CCWGG	6 cut(s)	395, 828, 1044, 1083, 1176, 1530
BstNSI	RCATGY	3 cut(s)	324, 653, 941
BstSCI	CCNGG	8 cut(s)	49, 178, 393, 826, 1042, 1081, 1174, 1528
BstSFI	CTRYAG	1 cut(s)	1215
BstUI	CGCG	1 cut(s)	1556
BstV1I	GCAGC	1 cut(s)	1174
BstX2I	RGATCY	1 cut(s)	430
BstYI	RGATCY	1 cut(s)	430
BstZ17I	GTATAC	1 cut(s)	925
Bsu36I	CCTNAGG	2 cut(s)	291, 567
BsuI	GTATCC	2 cut(s)	282, 1349
BsuRI	GGCC	3 cut(s)	114, 1344, 1773
BtgI	CCRYGG	2 cut(s)	75, 351
BtrI	CACGTC	1 cut(s)	698
BtsI	GCAGTG	1 cut(s)	751
BtsIMutI	CAGTG	2 cut(s)	241, 751
BveI	ACCTGC	2 cut(s)	1192, 1354
Cac8I	GCNNGC	5 cut(s)	385, 389, 552, 651, 1745
CciI	TCATGA	1 cut(s)	517
CfoI	GCGC	3 cut(s)	528, 1556, 1855
Cfr13I	GGNCC	2 cut(s)	1204, 1589
CseI	GACGC	1 cut(s)	899
Csp6I	GTAC	5 cut(s)	630, 941, 1132, 1212, 1862
CviAII	CATG	7 cut(s)	321, 352, 518, 650, 918, 938, 1712
CviQI	GTAC	5 cut(s)	630, 941, 1132, 1212, 1862
DdeI	CTNAG	6 cut(s)	186, 291, 567, 1032, 1115, 1694
DpnI	GATC	4 cut(s)	26, 432, 480, 1750
DpnII	GATC	4 cut(s)	24, 430, 478, 1748
DrdI	GACNNNNNNGTC	1 cut(s)	779
DseDI	GACNNNNNNGTC	1 cut(s)	779
EaeI	YGGCCR	2 cut(s)	112, 1342
Eam1104I	CTCTTC	2 cut(s)	495, 1818
EarI	CTCTTC	2 cut(s)	495, 1818
EciI	GGCGGA	1 cut(s)	54
Ecl136II	GAGCTC	1 cut(s)	44
Eco130I	CCWWGG	2 cut(s)	351, 1774
Eco24I	GRGCYC	2 cut(s)	46, 1813
Eco31I	GGTCTC	1 cut(s)	1454
Eco47I	GGWCC	2 cut(s)	1204, 1589
Eco47III	AGCGCT	1 cut(s)	1854
Eco53kI	GAGCTC	1 cut(s)	44
Eco57I	CTGAAG	2 cut(s)	1386, 1858
Eco81I	CCTNAGG	2 cut(s)	291, 567
EcoICRI	GAGCTC	1 cut(s)	44
EcoNI	CCTNNNNNAGG	2 cut(s)	566, 1173
EcoO109I	RGGNCCY	1 cut(s)	1204
EcoRI	GAATTC	1 cut(s)	802
EcoRII	CCWGG	6 cut(s)	393, 826, 1042, 1081, 1174, 1528
EcoT14I	CCWWGG	2 cut(s)	351, 1774
EcoT38I	GRGCYC	2 cut(s)	46, 1813
ErhI	CCWWGG	2 cut(s)	351, 1774
FaeI	CATG	7 cut(s)	324, 355, 521, 653, 921, 941, 1715
FaqI	GGGAC	4 cut(s)	161, 708, 1190, 1411
FatI	CATG	7 cut(s)	320, 351, 517, 649, 917, 937, 1711
FauI	CCCGC	1 cut(s)	1814
FauNDI	CATATG	1 cut(s)	1270
FblI	GTMKAC	1 cut(s)	924
Fnu4HI	GCNGC	5 cut(s)	34, 37, 115, 1188, 1377
FriOI	GRGCYC	2 cut(s)	46, 1813
Fsp4HI	GCNGC	5 cut(s)	34, 37, 115, 1188, 1377
FspBI	CTAG	5 cut(s)	443, 1170, 1209, 1817, 1859
GlaI	GCGC	3 cut(s)	527, 1555, 1854
GluI	GCNGC	5 cut(s)	34, 37, 115, 1188, 1377
GsaI	CCCAGC	1 cut(s)	408
GsuI	CTGGAG	2 cut(s)	899, 1365
HaeII	RGCGCY	1 cut(s)	1856
HaeIII	GGCC	3 cut(s)	114, 1344, 1773
HapII	CCGG	2 cut(s)	51, 180
HgaI	GACGC	1 cut(s)	899
HhaI	GCGC	3 cut(s)	528, 1556, 1855
Hin1II	CATG	7 cut(s)	324, 355, 521, 653, 921, 941, 1715
Hin6I	GCGC	3 cut(s)	526, 1554, 1853
HinP1I	GCGC	3 cut(s)	526, 1554, 1853
HincII	GTYRAC	1 cut(s)	254
HindII	GTYRAC	1 cut(s)	254
HindIII	AAGCTT	1 cut(s)	1257
HinfI	GANTC	5 cut(s)	465, 595, 862, 982, 1231
HpaII	CCGG	2 cut(s)	51, 180
HphI	GGTGA	3 cut(s)	583, 1097, 1560
Hpy166II	GTNNAC	3 cut(s)	254, 925, 1589
Hpy188I	TCNGA	4 cut(s)	189, 363, 1621, 1680
Hpy8I	GTNNAC	3 cut(s)	254, 925, 1589
Hpy99I	CGWCG	1 cut(s)	1805
HpyAV	CCTTC	3 cut(s)	440, 883, 1425
HpyCH4IV	ACGT	3 cut(s)	697, 1359, 1780
HpyCH4V	TGCA	9 cut(s)	324, 383, 554, 639, 833, 977, 1051, 1190, 1363
HpyF10VI	GCNNNNNNNGC	5 cut(s)	534, 1048, 1256, 1551, 1699
HpyF3I	CTNAG	6 cut(s)	186, 291, 567, 1032, 1115, 1694
HpySE526I	ACGT	3 cut(s)	697, 1359, 1780
Hsp92II	CATG	7 cut(s)	324, 355, 521, 653, 921, 941, 1715
HspAI	GCGC	3 cut(s)	526, 1554, 1853
Kzo9I	GATC	4 cut(s)	24, 430, 478, 1748
LguI	GCTCTTC	1 cut(s)	1818
LmnI	GCTCC	7 cut(s)	30, 41, 49, 163, 439, 880, 1384
Lsp1109I	GCAGC	1 cut(s)	1174
LweI	GCATC	3 cut(s)	203, 1038, 1815
MaeI	CTAG	5 cut(s)	443, 1170, 1209, 1817, 1859
MaeII	ACGT	3 cut(s)	697, 1359, 1780
MaeIII	GTNAC	6 cut(s)	230, 281, 770, 843, 1633, 1840
MalI	GATC	4 cut(s)	26, 432, 480, 1750
MbiI	CCGCTC	2 cut(s)	33, 1379
MboI	GATC	4 cut(s)	24, 430, 478, 1748
MboII	GAAGA	9 cut(s)	106, 512, 590, 761, 1109, 1234, 1426, 1634, 1805
MfeI	CAATTG	3 cut(s)	807, 1407, 1845
MflI	RGATCY	1 cut(s)	430
MhlI	GDGCHC	3 cut(s)	46, 885, 1813
MlsI	TGGCCA	1 cut(s)	1344
MluCI	AATT	9 cut(s)	474, 792, 802, 807, 1238, 1407, 1737, 1845, 1891
MluNI	TGGCCA	1 cut(s)	1344
MlyI	GAGTC	2 cut(s)	474, 1240
Mox20I	TGGCCA	1 cut(s)	1344
MroXI	GAANNNNTTC	1 cut(s)	1523
MscI	TGGCCA	1 cut(s)	1344
MseI	TTAA	3 cut(s)	278, 726, 1125
MslI	CAYNNNNRTG	4 cut(s)	211, 654, 918, 1476
Msp20I	TGGCCA	1 cut(s)	1344
MspI	CCGG	2 cut(s)	51, 180
MspR9I	CCNGG	8 cut(s)	51, 180, 395, 828, 1044, 1083, 1176, 1530
MunI	CAATTG	3 cut(s)	807, 1407, 1845
Mva1269I	GAATGC	2 cut(s)	1648, 1664
MvaI	CCWGG	6 cut(s)	395, 828, 1044, 1083, 1176, 1530
MvnI	CGCG	1 cut(s)	1556
MwoI	GCNNNNNNNGC	5 cut(s)	534, 1048, 1256, 1551, 1699
NciI	CCSGG	2 cut(s)	51, 180
NcoI	CCATGG	1 cut(s)	351
NdeI	CATATG	1 cut(s)	1270
NdeII	GATC	4 cut(s)	24, 430, 478, 1748
NlaIII	CATG	7 cut(s)	324, 355, 521, 653, 921, 941, 1715
NlaIV	GGNNCC	2 cut(s)	1206, 1534
NmuCI	GTSAC	4 cut(s)	230, 281, 770, 1840
NspI	RCATGY	3 cut(s)	324, 653, 941
PaeI	GCATGC	1 cut(s)	653
PagI	TCATGA	1 cut(s)	517
PaqCI	CACCTGC	1 cut(s)	1192
PciI	ACATGT	1 cut(s)	937
PciSI	GCTCTTC	1 cut(s)	1818
PcsI	WCGNNNNNNNCGW	1 cut(s)	1836
PctI	GAATGC	2 cut(s)	1648, 1664
PdmI	GAANNNNTTC	1 cut(s)	1523
PfeI	GAWTC	3 cut(s)	595, 862, 982
PflMI	CCANNNNNTGG	1 cut(s)	1274
PkrI	GCNGC	5 cut(s)	35, 38, 116, 1189, 1378
PleI	GAGTC	2 cut(s)	473, 1239
PpsI	GAGTC	2 cut(s)	473, 1239
Ppu21I	YACGTR	1 cut(s)	1360
PpuMI	RGGWCCY	1 cut(s)	1204
PscI	ACATGT	1 cut(s)	937
Psp124BI	GAGCTC	1 cut(s)	46
Psp5II	RGGWCCY	1 cut(s)	1204
Psp6I	CCWGG	6 cut(s)	393, 826, 1042, 1081, 1174, 1528
PspFI	CCCAGC	1 cut(s)	404
PspGI	CCWGG	6 cut(s)	393, 826, 1042, 1081, 1174, 1528
PspN4I	GGNNCC	2 cut(s)	1206, 1534
PspPI	GGNCC	2 cut(s)	1204, 1589
PspPPI	RGGWCCY	1 cut(s)	1204
PsuI	RGATCY	1 cut(s)	430
RsaI	GTAC	5 cut(s)	631, 942, 1133, 1213, 1863
RsaNI	GTAC	5 cut(s)	630, 941, 1132, 1212, 1862
RseI	CAYNNNNRTG	4 cut(s)	211, 654, 918, 1476
SacI	GAGCTC	1 cut(s)	46
SapI	GCTCTTC	1 cut(s)	1818
SaqAI	TTAA	3 cut(s)	278, 726, 1125
SatI	GCNGC	5 cut(s)	34, 37, 115, 1188, 1377
Sau3AI	GATC	4 cut(s)	24, 430, 478, 1748
Sau96I	GGNCC	2 cut(s)	1204, 1589
ScaI	AGTACT	1 cut(s)	1213
SchI	GAGTC	2 cut(s)	474, 1240
ScrFI	CCNGG	8 cut(s)	51, 180, 395, 828, 1044, 1083, 1176, 1530
SduI	GDGCHC	3 cut(s)	46, 885, 1813
SfaNI	GCATC	3 cut(s)	203, 1038, 1815
SfcI	CTRYAG	1 cut(s)	1215
SinI	GGWCC	2 cut(s)	1204, 1589
SmiMI	CAYNNNNRTG	4 cut(s)	211, 654, 918, 1476
SmlI	CTYRAG	1 cut(s)	1143
SmoI	CTYRAG	1 cut(s)	1143
SpeI	ACTAGT	1 cut(s)	1858
SphI	GCATGC	1 cut(s)	653
Sse9I	AATT	9 cut(s)	474, 792, 802, 807, 1238, 1407, 1737, 1845, 1891
SsiI	CCGC	6 cut(s)	33, 36, 39, 115, 1377, 1821
SspMI	CTAG	5 cut(s)	443, 1170, 1209, 1817, 1859
SstI	GAGCTC	1 cut(s)	46
StyD4I	CCNGG	8 cut(s)	49, 178, 393, 826, 1042, 1081, 1174, 1528
StyI	CCWWGG	2 cut(s)	351, 1774
TaiI	ACGT	3 cut(s)	700, 1362, 1783
TaqI	TCGA	2 cut(s)	427, 1803
TasI	AATT	9 cut(s)	474, 792, 802, 807, 1238, 1407, 1737, 1845, 1891
TatI	WGTACW	4 cut(s)	629, 1131, 1211, 1861
TauI	GCSGC	4 cut(s)	36, 39, 117, 1379
TfiI	GAWTC	3 cut(s)	595, 862, 982
Tru1I	TTAA	3 cut(s)	278, 726, 1125
Tru9I	TTAA	3 cut(s)	278, 726, 1125
TscAI	CASTG	2 cut(s)	241, 751
TseFI	GTSAC	4 cut(s)	230, 281, 770, 1840
TseI	GCWGC	1 cut(s)	1187
Tsp45I	GTSAC	4 cut(s)	230, 281, 770, 1840
TspDTI	ATGAA	6 cut(s)	862, 995, 1110, 1139, 1211, 1515
TspGWI	ACGGA	2 cut(s)	862, 1106
TspRI	CASTG	2 cut(s)	241, 751
Van91I	CCANNNNNTGG	1 cut(s)	1274
VpaK11BI	GGWCC	2 cut(s)	1204, 1589
XagI	CCTNNNNNAGG	2 cut(s)	566, 1173
XapI	RAATTY	1 cut(s)	802
XceI	RCATGY	3 cut(s)	324, 653, 941
XmiI	GTMKAC	1 cut(s)	924
XmnI	GAANNNNTTC	1 cut(s)	1523
XspI	CTAG	5 cut(s)	443, 1170, 1209, 1817, 1859
ZrmI	AGTACT	1 cut(s)	1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.