pycom08g19030

Threonylcarbamoyladenosine tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015293
Physical Location & Seq
Reverse (-)
19085608 .. 19089634
4027 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19030.2

Sequence Viewer

Length: 1056 bp
ATGTTACGGATAGGGATGACGAATCCTCCATTCATTCTGGAGCACTTGAAGGAAATAGCAGAGGTTTTGCGTCATCCATGTGTATACTCATTTCTACATGTACCAGTCCAATCTGGAAGTGATACAGTCTTGACTGCAATGAATCGGGAATATACCGTAAGTGAGTTCAAGACTGTGGTAGATACCCTAACTGAGCTTGTGCCAGGGATGCAGATTGCGACTGATATAATATGTGGATTTCCTGGTGAAACGGATGAAGACTTTACTCAAACTCTTAGCCTTATTAAAGAGTACAAGTTCTCTCAAGTTCATATTTCGCAGTTTTATCCTAGACCAGGAACACCTGCTGCAAGGATGAAAAAGGTCCCCAGTACTGTAGTGAAGAAAAGGAGTCGTGAATTGACTTCTGCTTTTGAAGCTTTCGCACCATATGTTGGGATGGAGGGCAGGGTGGAAAGAATATGGATAACTGATATTGCGACTGATGGAACTCACTTGGTTGGCCATACAAAGGGATACGTGCAGGTTCTTGTAGCCGCTCCAGAAAGTATGTTGGGGACTTCAGCAATTGTGAAGATAACATCGGTGGGAAGGTGGTCGGTTTTTGGAGAAGTGATTGAGACCATCCCTCACATAAACGATAAACCCGCCTCAACAAATGAAAACCGTAGTCAGGAGAAATGTTTCCCTGGTGCCAACAGTTGCGAGACTTGCGCGTGTTCAACTTCGCCAGAAACTTGTGCCTGTGGACCAGAAAGCTGTGGAGGACAGGCTACCTCGGAAGAATGTGCTGTAACAAAGAATGCCGTTCTGCTGGAACACTGGAACAGTAGAAATCCGATTGGATGGTTACTGAGGAAGCGAAAGAACCATGTTGAGAAACAGGTGGAGGATGAAATTGGCTTGCGATCCCAGAACAAGCAAGAGCAAGCCCAAGGATGTTTGAGTGATTGGGGTTTTGTCGATAGGGCTCTTCTAGGCGGGATGCTCATGAGCTTCGTGACAATTGTAGCGCTACTAGTACATCTTGGCTTTAGGATTATGTCGTCTAATTAG

Protein Analysis

352

Amino Acids

38.75

Weight (kDa)

6.09

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015293)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72090
fragaria_vesca FvH4_5g32030
malus_domestica MD08G1219300.v1.1 MD15G1407900.v1.1
prunus_persica Prupe.1G553700_v2.0.a1
pyrus_communis pycom08g19030 pycom15g36400
rosa_chinensis RchiOBHm_Chr7g0228031
rosa_laevigata RLG00000001594
rosa_multiflora Rmu_sc0004858.1_g000015
rosa_roxburghii Rroxscaffold_3G00231640
rosa_rugosa Rorug07G0245400
rosa_samantha Rh7AG399200 Rh7CG417300 Rh7DG394700
rosa_wichuraiana Rw7G033180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 2 cut(s) 352, 514
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 539
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 716
AciI CCGC 3 cut(s) 537, 648, 981
AclWI GGATC 1 cut(s) 903
AcoI YGGCCR 1 cut(s) 502
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 4 cut(s) 102, 293, 373, 1023
AfeI AGCGCT 1 cut(s) 1014
AfiI CCNNNNNNNGG 4 cut(s) 335, 434, 511, 673
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 4 cut(s) 49, 169, 416, 723
AhlI ACTAGT 1 cut(s) 1018
AjnI CCWGG 4 cut(s) 202, 241, 334, 688
AluBI AGCT 4 cut(s) 196, 419, 759, 996
AluI AGCT 4 cut(s) 196, 419, 759, 996
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 2 cut(s) 614, 701
AlwI GGATC 1 cut(s) 903
Aor51HI AGCGCT 1 cut(s) 1014
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 683
AspLEI GCGC 2 cut(s) 716, 1015
AspS9I GGNCC 2 cut(s) 364, 749
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 2 cut(s) 364, 749
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 1 cut(s) 973
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 334
BccI CCATC 4 cut(s) 433, 479, 632, 840
BceAI ACGGC 1 cut(s) 791
BciT130I CCWGG 4 cut(s) 204, 243, 336, 690
BciVI GTATCC 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 614, 701
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 3 cut(s) 330, 977, 1019
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 1016
BfuAI ACCTGC 2 cut(s) 352, 514
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 2 cut(s) 348, 537
BlsI GCNGC 2 cut(s) 349, 538
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 4 cut(s) 204, 243, 336, 690
Bme18I GGWCC 2 cut(s) 364, 749
BmgT120I GGNCC 2 cut(s) 364, 749
BmiI GGNNCC 2 cut(s) 366, 694
BmrFI CCNGG 4 cut(s) 204, 243, 336, 690
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 198, 975
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 2 cut(s) 59, 525
BpuEI CTTGAG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 520
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 4 cut(s) 203, 688, 777, 934
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 434, 511, 673
Bse1I ACTGG 3 cut(s) 104, 369, 827
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 4 cut(s) 204, 243, 336, 690
BseDI CCNNGG 4 cut(s) 203, 688, 777, 934
BseLI CCNNNNNNNGG 4 cut(s) 335, 434, 511, 673
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 183, 845
BseNI ACTGG 3 cut(s) 104, 369, 827
BseXI GCAGC 1 cut(s) 334
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 1 cut(s) 504
BshNI GGYRCC 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 45
BslFI GGGAC 2 cut(s) 350, 571
BslI CCNNNNNNNGG 4 cut(s) 335, 434, 511, 673
BsmAI GTCTC 2 cut(s) 614, 701
BsmFI GGGAC 2 cut(s) 350, 571
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 1 cut(s) 504
Bso31I GGTCTC 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 45, 973
Bsp143I GATC 1 cut(s) 908
BspACI CCGC 3 cut(s) 537, 648, 981
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 2 cut(s) 184, 846
BspFNI CGCG 1 cut(s) 716
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 366, 694
BspMI ACCTGC 2 cut(s) 352, 514
BspPI GGATC 1 cut(s) 903
BspQI GCTCTTC 1 cut(s) 978
BspT107I GGYRCC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 3 cut(s) 104, 369, 827
BssECI CCNNGG 4 cut(s) 203, 688, 777, 934
BssMI GATC 1 cut(s) 908
BssNAI GTATAC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 934
Bst1107I GTATAC 1 cut(s) 85
Bst2UI CCWGG 4 cut(s) 204, 243, 336, 690
Bst4CI ACNGT 7 cut(s) 127, 157, 175, 376, 668, 701, 830
Bst6I CTCTTC 1 cut(s) 978
BstBAI YACGTR 1 cut(s) 520
BstC8I GCNNGC 2 cut(s) 905, 930
BstDEI CTNAG 3 cut(s) 192, 275, 854
BstENI CCTNNNNNAGG 1 cut(s) 333
BstFNI CGCG 1 cut(s) 716
BstH2I RGCGCY 1 cut(s) 1016
BstHHI GCGC 2 cut(s) 716, 1015
BstKTI GATC 1 cut(s) 911
BstMAI GTCTC 2 cut(s) 614, 701
BstMBI GATC 1 cut(s) 908
BstMWI GCNNNNNNNGC 3 cut(s) 208, 416, 711
BstNI CCWGG 4 cut(s) 204, 243, 336, 690
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 4 cut(s) 202, 241, 334, 688
BstSFI CTRYAG 1 cut(s) 375
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 1 cut(s) 334
BstV2I GAAGAC 1 cut(s) 264
BstZ17I GTATAC 1 cut(s) 85
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 820
BveI ACCTGC 2 cut(s) 352, 514
Cac8I GCNNGC 2 cut(s) 905, 930
CciI TCATGA 1 cut(s) 990
CfoI GCGC 2 cut(s) 716, 1015
Cfr13I GGNCC 2 cut(s) 364, 749
CseI GACGC 1 cut(s) 59
Csp6I GTAC 4 cut(s) 101, 292, 372, 1022
CviAII CATG 4 cut(s) 78, 98, 872, 991
CviQI GTAC 4 cut(s) 101, 292, 372, 1022
DdeI CTNAG 3 cut(s) 192, 275, 854
DpnI GATC 1 cut(s) 910
DpnII GATC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Eco130I CCWWGG 1 cut(s) 934
Eco24I GRGCYC 1 cut(s) 973
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 2 cut(s) 364, 749
Eco47III AGCGCT 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 546
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 364
EcoRII CCWGG 4 cut(s) 202, 241, 334, 688
EcoT14I CCWWGG 1 cut(s) 934
EcoT38I GRGCYC 1 cut(s) 973
ErhI CCWWGG 1 cut(s) 934
FaeI CATG 4 cut(s) 81, 101, 875, 994
FaqI GGGAC 2 cut(s) 350, 571
FatI CATG 4 cut(s) 77, 97, 871, 990
FauI CCCGC 2 cut(s) 655, 974
FauNDI CATATG 1 cut(s) 430
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 348, 537
FriOI GRGCYC 1 cut(s) 973
Fsp4HI GCNGC 2 cut(s) 348, 537
FspBI CTAG 3 cut(s) 330, 977, 1019
GlaI GCGC 2 cut(s) 715, 1014
GluI GCNGC 2 cut(s) 348, 537
GsuI CTGGAG 2 cut(s) 59, 525
HaeII RGCGCY 1 cut(s) 1016
HaeIII GGCC 1 cut(s) 504
HgaI GACGC 1 cut(s) 59
HhaI GCGC 2 cut(s) 716, 1015
Hin1II CATG 4 cut(s) 81, 101, 875, 994
Hin6I GCGC 2 cut(s) 714, 1013
HinP1I GCGC 2 cut(s) 714, 1013
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 3 cut(s) 22, 142, 391
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 85, 749
Hpy188I TCNGA 2 cut(s) 781, 840
Hpy8I GTNNAC 2 cut(s) 85, 749
HpyAV CCTTC 2 cut(s) 43, 585
HpyCH4III ACNGT 7 cut(s) 127, 157, 175, 376, 668, 701, 830
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 137, 211, 350, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 416, 711
HpyF3I CTNAG 3 cut(s) 192, 275, 854
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 4 cut(s) 81, 101, 875, 994
HspAI GCGC 2 cut(s) 714, 1013
Kzo9I GATC 1 cut(s) 908
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 2 cut(s) 40, 544
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 2 cut(s) 198, 975
MaeI CTAG 3 cut(s) 330, 977, 1019
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 4 cut(s) 3, 793, 849, 1000
MalI GATC 1 cut(s) 910
MbiI CCGCTC 1 cut(s) 539
MboI GATC 1 cut(s) 908
MboII GAAGA 5 cut(s) 269, 394, 586, 794, 965
MfeI CAATTG 2 cut(s) 567, 1005
MhlI GDGCHC 2 cut(s) 45, 973
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 5 cut(s) 398, 567, 897, 1005, 1051
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 1 cut(s) 400
MnlI CCTC 9 cut(s) 36, 55, 436, 639, 661, 758, 787, 849, 883
Mox20I TGGCCA 1 cut(s) 504
MroXI GAANNNNTTC 1 cut(s) 683
MscI TGGCCA 1 cut(s) 504
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 504
MspR9I CCNGG 4 cut(s) 204, 243, 336, 690
MunI CAATTG 2 cut(s) 567, 1005
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 4 cut(s) 204, 243, 336, 690
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 3 cut(s) 208, 416, 711
NdeI CATATG 1 cut(s) 430
NdeII GATC 1 cut(s) 908
NlaIII CATG 4 cut(s) 81, 101, 875, 994
NlaIV GGNNCC 2 cut(s) 366, 694
NmuCI GTSAC 1 cut(s) 1000
NspI RCATGY 1 cut(s) 101
PagI TCATGA 1 cut(s) 990
PaqCI CACCTGC 1 cut(s) 352
PciI ACATGT 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 2 cut(s) 22, 142
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 2 cut(s) 349, 538
PleI GAGTC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 399
Ppu21I YACGTR 1 cut(s) 520
PpuMI RGGWCCY 1 cut(s) 364
PscI ACATGT 1 cut(s) 97
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 4 cut(s) 202, 241, 334, 688
PspGI CCWGG 4 cut(s) 202, 241, 334, 688
PspN4I GGNNCC 2 cut(s) 366, 694
PspPI GGNCC 2 cut(s) 364, 749
PspPPI RGGWCCY 1 cut(s) 364
RsaI GTAC 4 cut(s) 102, 293, 373, 1023
RsaNI GTAC 4 cut(s) 101, 292, 372, 1022
RseI CAYNNNNRTG 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 348, 537
Sau3AI GATC 1 cut(s) 908
Sau96I GGNCC 2 cut(s) 364, 749
ScaI AGTACT 1 cut(s) 373
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 4 cut(s) 204, 243, 336, 690
SduI GDGCHC 2 cut(s) 45, 973
SfaNI GCATC 2 cut(s) 198, 975
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 2 cut(s) 364, 749
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SpeI ACTAGT 1 cut(s) 1018
Sse9I AATT 5 cut(s) 398, 567, 897, 1005, 1051
SsiI CCGC 3 cut(s) 537, 648, 981
SspMI CTAG 3 cut(s) 330, 977, 1019
StyD4I CCNGG 4 cut(s) 202, 241, 334, 688
StyI CCWWGG 1 cut(s) 934
TaaI ACNGT 7 cut(s) 127, 157, 175, 376, 668, 701, 830
TaiI ACGT 1 cut(s) 522
TaqI TCGA 1 cut(s) 963
TasI AATT 5 cut(s) 398, 567, 897, 1005, 1051
TatI WGTACW 3 cut(s) 291, 371, 1021
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 22, 142
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 827
TseFI GTSAC 1 cut(s) 1000
TseI GCWGC 1 cut(s) 347
Tsp45I GTSAC 1 cut(s) 1000
TspDTI ATGAA 7 cut(s) 22, 155, 270, 299, 371, 675, 909
TspGWI ACGGA 2 cut(s) 22, 266
TspRI CASTG 1 cut(s) 827
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 364, 749
XagI CCTNNNNNAGG 1 cut(s) 333
XceI RCATGY 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 3 cut(s) 330, 977, 1019
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.